Membrane transport
Subtitle: a practical approach
By: edited by Stephen A. Baldwin
Series: The practical approach series -- 230; Practical approach series -- 230.
Publisher: Oxford University Press
Published: 2000
Place of publication: Oxford; New York
Language: eng
Number of pages: 320
ISBN-13: 9780191565823
Subjects: Carrier proteins; Transporte através da membrana; Membrantransport; Biochemistry; SCIENCE; Cells; Cell Membrane; Transportverschijnselen; Eiwitten; Cell membranes; Laboratory Manuals; Metabolism; Carrier Proteins; Life Sciences; Cytological Techniques; Biological transport; Biomoleculen; Experiments; Protein Transport; Membranen; Membranas celulares; Laboratory manuals; Physiology
Description: Membrane Transport contains a collection of experimental protocols for the study of proteins responsible for the transport of small molecules across biological membranes. It includes information on the latest methods for production of transport proteins by recombinant DNA technology, and for their investigation by techniques accessible to any well-found laboratory.
Notes: Includes bibliographical references and index.
Table of contents: title: Assay of membrane transport in cells and membrane vesicles / Simon M. Jarvis; title: Reconstitution of membrane proteins : the Ca²-ATPase of sarcoplasmic reticulum / Anthony G. Lee; title: The Xenopus oocyte expression system for the cDNA cloning and characterization of plasma membrane transport proteins / Sylvia Y.M. Yao, Carol E. Cass, and James D. Young; title: Expression of foreign transport proteins in yeast / N. Sauer and J. Stolz; title: Baculovirus-mediated overexpression of transport proteins / Gary J. Litherland and Stephen A. Baldwin; title: The amplified expression, identification, purification, assay, and properties of hexahistidine-tagged bacterial membrane transport proteins / Alison Ward [and others]; title: Spectroscopic and kinetic approaches for probing the mechanisms of solute transporters / Adrian R. Walmsley; title: Detection and analysis of glucose transporters using photolabelling techniques / Alison K. Gillingham [and others]; title: Computer prediction of transporter topology and structure / Rong-I Hong and Mark S.P. Sansom; title: Two-dimensional crystallization of membrane proteins / Philippe Ringler, Bernard Heymann, and Andreas Engel; title: Crystallization of membrane proteins / Tina D. Howard, Katherine E. McAuley-Hecht, and Richard J. Cogdell.