Ribosome display and related technologies
Subtitle: methods and protocols
By: edited by Julie A. Douthwaite and Ronald H. Jackson
Series: Methods in molecular biology -- 805; Springer protocols; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 805.
Publisher: Humana Press
Published: 2012
Place of publication: New York
Language: eng
Number of pages: 424
ISBN-13: 9781617793783; 9781617793790
Subjects: Protein Engineering; Gene Expression Profiling; Methods; Laboratory manuals; RNA-protein interactions; Ribosomal Proteins; Research; Ribosomes
Notes: Includes bibliographical references and index.
Table of contents: title: Ribosome display : a perspective / Andreas Plückthun; title: Preparation and testing of E. coli S30 in vitro transcirption translation extracts / James F. Zawada; title: Eurkaryotic ribosome display selection using rabbit reticulocyte lysate / Julie A Douthwaite; title: Stabilized ribosome display for in vitro selection / Shuta Hara ... [et al.]; title: Eukaryotic ribosome display with in situ DNA recovery / Mingyue He ... [et al.]; title: mRNA display using covalent coupling of mRNA to translated proteins / Rong Wang, Steve W. Cotten, and Rihe Liu; title: SNAP display : in vitro rotein evolution in microdroplets / Miriam Kaltenbach and Florian Hollfelder; title: cDNA display : rapid stabilization of mRNA display / Shingo Ueno and Naoto Nemoto; title: Optimisation of antibody affinity by ribosome display using error-prone or site-directed mutagenesis / Leeanne Lewis and Chris Lloyd; title: Affinity maturation of phage display antibody populations using ribosome display / Maria A. Groves and Adrian A. Nickson; title: Evolution of protein stability using ribosome display / Andrew Buchanan; title: Selection of lead antibodies from naive ribosome display antibody libraries / Peter Ravn; title: Evolution of disulfide-rich peptide aptamers using cDNA display / Yuki Mochizuki and Naoto Nemoto; title: Peptide screening using PURE ribosome display / Hiroyuki Ohashi ... [et al.]; title: Rapid selection of high-affinity binders using ribosome display / Birgit Dreier and Andreas Plückthun; title: mRNA display-based selections using synthetic peptide and natural protein libraries / Steve W. Cotten ... [et al.]; title: Identification of candidate vaccine genes using ribosome display / Liancheng Lei; title: Ribosome display for the selection of Sac7d scaffolds / Barbara Mouratou ... [et al.]; title: Charging of tRNAs using ribozymes and selection of cyclic peptides containing thioethers / Patrick C. Reid ... [et al.]; title: Update on pure translation display with unnatural amino acid incorporation / R. Edward Watts and Anthony C. Forster; title: In vitro selection of unnatural cyclic peptide libraries via mRNA display / Zhong Ma and Matthew C.T. Hartman; title: Optimization of CAT-354, a therapeutic antibody directed against interleukin-13, using ribosome display / George Thom and Ralph Minter; title: Affinity maturation and functional dissection of a humanised anti-RAGE monoclonal antibody by ribosome display / Simon E. Hufton.