Plant transposable elements
Subtitle: methods and protocols
By: edited by Thomas Peterson, Department of Agronomy, Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, USA
Series: Methods in molecular biology -- 1057; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 1057.; Springer protocols (Series)
Publisher: Humana Press
Published: 2013
Place of publication: New York
Language: eng
Number of pages: 324
ISBN-13: 9781627035675; 9781627035682
Copyright date: 2013
Subjects: Transposons; Laboratory manuals; Plant genetic transformation; DNA Transposable Elements; Laboratory Manuals; Plant Genome; Genetics
Description: "Transposable elements have played a major role in shaping plant genome structure and gene expression.  Transposons not only drive sequence expansion, induce mutations and generate chromosome rearrangements, they also help to shape the epigenetic topology of the eukaryotic genome.  In Plant Transposable Elements: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study transposons. These methods include computational approaches to study the ancient transposon remnants that comprise the bulk of plant genomes, as well as laboratory techniques to identify recent and ongoing transposition events." --
Notes: Includes bibliographical references and index.
Table of contents: title: Historical overview of transposable element research  -- P.A. Peterson; title: Distinguishing variable phenotypes from variegation caused by transposon activities  -- V. Walbot; title: Using transposons for genetic mosaic analysis of plant development  -- P.W. Becraft; title: Survey of natural and transgenic gene markers used to monitor transposon activity  -- L. Krishnaswamy and T. Peterson; title: Molecular biology of maize Ac/Ds elements: An overview  -- K. Lazarow, M.L. Doll and R. Kunze; title: Gene tagging with engineered Ds elements in maize  -- Y. Li, G. Segal, Q. Wangm H.K. Donner; title: Plant regeneration methods for rapid generation of a large scale Ds transposant population in rice  -- Y.H. Xuan [and nine others]; title: Isolation of sequences flanking Ac insertion sites by Ac casting  -- D. Wang and T. Peterson; title: Regulation of the mutator system of transposons in maize  -- D. Lisch; title: Using MuDR/Mu transposons in directed tagging strategies  -- V. Walbot and J. Questa; title: Genetic and molecular analyses of UniformMu transposon insertion lines  -- D.R. McCarty, M. Suzuki, C. Hunter, J. Collins, W.T. Avigne, K.E. Koch; title: Digestion-ligation-amplification (DLA): A simple genome walking method to amplify unknown sequences flanking mutator (Mu) transposons and thereby facilitate gene cloning  -- S. Liu, A.P. Hsia and P.S. Schnable; title: Molecular genetics and epigenetics of CACTA elements  -- N.V. Fedoroff; title: Activation tagging using the maize En-I transposon system for the identification of abiotic stress resistance genes in arabidopsis  -- A. Harb and A. Pereira; title: Reverse genetics in rice using Tos17  -- D. Mieulet, A. Dievart, G. Droc, N. Lanau, E. Guiderdoni; title: Identification and applications of the Petunia Class II Act1/dTph1 transposable element system  -- T. Gerats, J. Zethof and M. Vandenbussche; title: Transposon display: A versatile method for transposon tagging  -- M. Vandenbussche, J. Zethof and T. Gerats; title: Massive indexed parallel identification of transposon flanking sequences  -- M. Vandenbussche, J. Zethof and T. Gerats; title: Use of next generation sequencing (NGS) technologies for the genome-wide detection of transposition  -- M. Elbaidouri, C. Chaparro and O. Panaud; title: Overview of repeat annotation and de novo repeat identification  -- N. Jiang; title: Computational methods for identification of DNA transposons  -- N. Jiang; title: Tenest 2.0: Computational annotation and visualization of nested transposable elements -- B.A. Kronmiller and R.P. Wise.