Mycobacteria protocols
By: edited by Tanya Parish and David M. Roberts, TB Discovery Research, Infectious Disease Research Institute (IDRI), Seattle, WA, USA
Edition: Third edition.
Series: Methods in molecular biology -- 1285; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 1285,; Springer protocols (Series)
Publisher: Humana Press
Published: 2015
Place of publication: New York
Language: eng
Number of pages: 416
ISBN-13: 9781493924493; 9781493924509
Copyright date: 2015
Subjects: Genetics; Bacterial DNA; Mycobacterium; Laboratory Manuals; Growth & development; Mycobacteria; Laboratory manuals; Bacterial Proteins
Notes: Includes bibliographical references and index.
Table of contents: title: Whole-genome sequencing for comparative genomics and de novo genome assembly  -- Andrej Benjak, Claudia Sala, and Ruben C. Hartkoorn; title: Whole-transcriptome sequencing for high-resolution transcriptomic analysis in mycobacterium tuberculosis  -- Andrej Benjak, Claudia Sala, and Ruben C. Hartkoorn; title: RNA sequencing for transcript 5'-end mapping in mycobacteria  -- Scarlet S. Shell [and 3 otherst]; title: Fractionation and analysis of mycobacterial proteins  -- Megan C. Lucas [and 6 others]; title: Lipid and lipoarabinomannan isolation and characterization  -- Marie-Antoinette Lanéelle, Jérôme Nigou, and Mamadou Daffé; title: Metabolomics of mycobacterium tuberculosis  -- Madhumitha Nandakumar [and 3 others]; title: Electroporation of mycobacteria  -- Renan Goude, David M. Roberts, and Tanya Parish; title: Targeted gene knockout and essentiality testing by homologous recombination  -- Krishnamoorthy Gopinath, Digby F. Warner, and Valerie Mizrahi; title: Construction of conditional knockdown mutants in mycobacteria  -- Dirk Schnappinger, Kathryn M. O'Brien, and Sabine Ehrt; title: Mycobacterial recombineering  -- Kenan C. Murphy, Kadamba Papavinasasundaram, and Christopher M. Sassetti; title: In vitro models that utilize hypoxia to induce non-replicating persistence in mycobacteria  -- Charles D. Sohaskeyand Martin I. Voskuil; title: Genetic dissection of mycobacterial biofilms  -- Anil K. Ojha, William R. Jacobs Jr., and Graham F. Hatfull; title: Measuring efflux and permeability in mycobacteria  -- Liliana Rodrigues, Miguel Viveiros, and José A. Aínsa; title: Single-cell analysis of mycobacteria using microfluidics and time-lapse microscopy  -- Neeraj Dharand and Giulia Manina; title: Antimicrobial susceptibility testing for Mycobacterium sp.  -- Delia Blanco-Ruano [and 6 others]; title: Determination of compound kill kinetics against mycobacterium tuberculosis  -- Julie Early and Torey Alling; title: Microplate alamar blue assay (MABA) and low oxygen recovery assay (LORA) for mycobacterium tuberculosis -- Sanghyun Cho, Hyung Sup Lee, and Scott Franzblau; title: Multi-stress model for high throughput screening against non-replicating mycobacterium tuberculosis  -- Ben Gold, Thulasi Warrier, and Carl Nathan; title: Isolation and characterization of compound-resistant isolates of mycobacterium tuberculosis  -- Theresa O'Malley and Eduard Melief; title: Macrophage infection models for mycobacterium tuberculosis  -- Benjamin K. Johnson and Robert B. Abramovitch; title: Infection of human neutrophils to study virulence properties of mycobacterium tuberculosis  -- Tobias Dallenga, Björn Corleis, and Ulrich E. Schaible; title: Isolation of bead phagosomes to study virulence function of M. tuberculosis cell wall lipids  -- Anna C. Geffken, Emmanuel C. Patin, and Ulrich E. Schaible; title: Live imaging of mycobacterium marinum infection in dictyostelium discoideum  -- Caroline Barisch, Ana T. López-Jiménez, and Thierry Soldati; title: Testing chemical and genetic modulators in mycobacterium tuberculosis infected cells using phenotypic assays -- Vincent Delorme [and 3 others].