Glucagon-like peptide-1-induced suppression on glucagon secretion in pancreatic alpha-cells: A study using phosphoinositol 3-kinasegamma deficient mice.
Published: 2006
Language: eng
Number of pages: 94
ISBN-13: 9780494213735
Description: Glucagon-like peptide-1 (GLP-1), potentiates glucose-stimulated insulin Secretion from pancreatic beta-cells and inhibits glucagon release from pancreatic alpha-cells. In beta-cells, GLP-1-stimulated insulin secretion is partly mediated by cAMP-dependent and phosphoinositide 3-kinase (PI3K) dependent pathways. While much is known about beta-cell signaling mechanisms, how GLP-1 suppresses alpha-cell glucagon secretion remains largely unknown. Given that GLP-1 receptor is a G-protein coupled receptor (GPCR), mice lacking PI3Kgamma, a GPCR-activated isoform, were used to examine the mechanism(s) underlying GLP-1 suppression of glucagon secretion. RT-PCR and immunocytochemistry failed to detect GLP-1 receptor in glucagon-secreting alpha-TC6, InR1-G9, and murine alpha-cells. Pancreas perfusion of a GLP-1 analogue suppressed glucagon secretion in wild-type and PI3Kgamma -/- mice in a wortmannin insensitive manner. Furthermore, insulin was found to suppress glucagon secretion both  in vitro and ex vivo, mimicking the actions of GLP-1. Therefore, GLP-1-induced glucagon suppression is likely secondary to insulin's actions on alpha-cells and independent of PI3Kgamma pathway.
Notes: Source: Masters Abstracts International, Volume: 45-03, page: 1400.

Thesis (M.Sc.)--University of Toronto, 2006.

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