Design of a genetic differential amplifier.
Published: 2004
Language: eng
Number of pages: 141
Description: The circuit was modeled using a statistical thermodynamic and stochastic approach. Simulations indicated that the circuit displayed the desired behaviour when two mutations were introduced into the promoter. One mutation added a bias to the circuit, and the other mutation made CI strictly an activator.High and low copy number variants of the amplifier plasmid were constructed and tested using several different input plasmids. A non-inverting response was observed, but an inverting response was not.A genetic analog to an electronic differential amplifier circuit was developed using components from bacteriophage lambda. A differential amplifier produces an output that is proportional to the difference between the two inputs. The activator protein CI was used as the non-inverting (positive) input. The repressor protein Cro was used as the inverting (negative) input. Both of the input proteins bound to the PRM promoter whose activity was monitored using enhanced green fluorescent protein.
Notes: Adviser:  Stephen Davies.

Thesis (M.A.Sc.)--University of Toronto, 2004.

Electronic version licensed for access by U. of T. users.

Source: Masters Abstracts International, Volume: 43-03, page: 0904.

MICR copy on microfiche (2 microfiches).