Immune homeostasis
Subtitle: methods and protocols
By: edited by Andrew L. Snow, Michael J. Lenardo
Series: Methods in molecular biology -- 979; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 979.; Springer protocols
Publisher: Humana Press; Springer
Published: 2013
Place of publication: New York
Language: eng
Number of pages: 215
ISBN-13: 9781627032896; 9781627032902
Subjects: Immunology; Laboratory manuals; Immune System; Laboratory Manuals; Homeostasis; Laboratory Manuals..
Notes: Includes bibliographical references and index.
Table of contents: title: Quantitating lymphocyte programmed cell death in vitro using simple kill assays  -- Lixin Zheng; title: Fluorescence-activated cell sorting-based quantitation of T cell receptor restimulation-induced cell death in activated, primary human T cells  -- Gil Katz and Andrew L. Snow; title: Evaluation of IL-2-withdrawal-induced apoptosis in human T lymphocytes  -- Joao Bosco Oliveira; title: Determination of apoptosis sensitivity in specific T cell subsets from human peripheral blood by utilizing a multiparameter fluorescence-activated cell sorting-based technique  -- Bernice Lo and Madhu Ramaswamy; title: Visualization of fas-mediated death-inducing signaling complex formation by immunoprecipitation  -- Nelia Cordeiro and Nicolas Bidère; title: Analyses of programmed cell death in dendritic cells  -- Min Chen, Lily Huang, and Jin Wang -- Detection of necrosis by release of lactate dehydrogenase activity / -- Francis Ka-Ming Chan, Kenta Moriwaki, and María José De Rosa; title: Assessment of CD4+ and CD8+ T cell responses using MHC class I and II tetramers  -- Sema Kurtulus and David Hildeman; title: Homeostatic proliferation of mature T cells  -- Christopher E. Martin ... [et al.]; title: Quantitating lymphocyte homeostasis in vivo in humans using stable isotope tracers  -- Liset Westera ... [et al.]; title: Real-time quantitative (RQ-)PCR approach to quantify the contribution of proliferation to B lymphocyte homeostasis  -- Menno C. van Zelm ... [et al.]; title: Molecular measurement of T cell receptor excision circles  -- Heather E. Lynch and Gregory D. Sempowski; title: Isolation of RNA and the synthesis and amplification of cDNA from antigen-specific T cells for genome-wide expression analysis  -- R. Anthony Barnitz ... [et al.]; title: Designs for massively parallel sequencing approaches to identify causal mutations in human immune disorders  -- Yu Zhang and Helen C. Su; title: Flow cytometric measurement of SLAM-associated protein and X-linked inhibitor of apoptosis  -- Rebecca A. Marsh, Jack J. Bleesing, and Alexandra H. Filipovich; title: In vitro suppression assay for functional assessment of human regulatory T cells -- Dat Q. Tran.