Neural stem cells
Subtitle: methods and protocols
By: edited by Tanja Zigova, Paul R. Sanberg, Juan R. Sanchez-Ramos.
Contributors: Zigova, Tanja.; Sanberg, Paul R.; Sanchez-Ramos, Juan Raymond, 1945-
Series: Methods in molecular biology -- 198; Methods in molecular biology (Clifton, N.J.) -- v. 198.
Publisher: Humana Press
Published: 2002
Place of publication: Totowa, NJ
Language: eng
Number of pages: 381
Subjects: Neural stem cells -- Laboratory manuals
Genres: Laboratory manuals.
Notes: Includes bibliographical references and index.
Table of contents: title: Machine generated contents note: Prefacev; title: Contributors xi; title: PART I ISOLATION AND CULTURE OF NSCs; title: 1 Neural Differentiation of Embryonic Stem Cells; title: K. Sue O'Shea; title: 2 Production and Analysis of Neurospheres from Acutely Dissociated and Postmortem CNS Specimens; title: Eric D. Laywell, Valery G. Kukekov, Oleg Suslov,; title: Tong Zheng, and Dennis A. Steindler 15; title: 3 Isolation of Stem and Precursor Cells from Fetal Tissue; title: Yuan Y. Wu, Tahmina Mujtaba, and Mahendra S. Rao29; title: 4 Olfactory Ensheathing Cells: Isolation and Culture from the Rat Olfactory Bulb; title: Susan C. Barnett and A. Jane Roskams 41; title: 5 Culturing Olfactory Ensheathing Glia from the Mouse Olfactory Epithelium; title: Edmund Au and A. Jane Roskams49; title: 6 Production of Immortalized Human Neural Crest Stem Cells; title: Seung U. Kim, Eiji Nakagawa, Kozo Hatori, Atsushi Nagai,; title: Myung A. Lee, and Jung H. Bang55; title: 7 Adult Rodent Spinal Cord Derived Neural Stem Cells: Isolation and Characterization; title: Lamya S. Shihabuddin67; title: 8 Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood; title: Shijie Song and J. Sanchez-Ramos79; title: 9 Seeding Neural Stem Cells on Scaffolds of PGA, PLA, and Their Copolymers; title: Erin Lavik, Yang D. Teng, Evan Snyder, and Robert Langer 89; title: PART II CHARACTERIZATION OF NSCs IN VITRO; title: A. CELLULAR TECHNIQUES; title: 10 Analysis of Cell Generation in the Telencephalic Neuroepithelium; title: Takao Takahashi, Verne S. Caviness, Jr.,; title: and Pradeep G. Bhide101; title: 11 Clonal Analyses and Cryopreservation; title: of Neural Stem Cell Cultures; title: Angelo L. Vescovi, Rossella Galli, and Angela Gritti115; title: 12 Assessing the Involvement of Telomerase in Stem Cell Biology; title: Mark P. Mattson, Peisu Zhang, and Weiming Fu 125; title: 13 Detection of Telomerase Activity in Neural Cells; title: Karen R. Prowse137; title: 14 In Vitro Assays for Neural Stem Cell Differentiation; title: Marcel M. Daadi 149; title: 15 Electron Microscopy and Lac-Z Labeling; title: Bela Kosaras and Evan Snyder157; title: B. ELECTROPHYSIOLOGICAL TECHNIQUES; title: 16 Techniques for Studying the Electrophysiology of Neurons Derived from Neural Stem/Progenitor Cells; title: David S. K. Magnuson and Dante J. Morassutti179; title: C. MOLECULAR TECHNIQUES; title: 17 Fluorescence In Situ Hybridization; title: Barbara A. Tate and Rachel L. Ostroff 189; title: 18 RT-PCR Analyses of Differential Gene Expression; title: in ES-Derived Neural Stem Cells; title: Theresa E. Gratsch197; title: 19 Differential Display: Isolation of Novel Genes; title: Theresa E. Gratsch213; title: 20 Cell Labeling and Gene Misexpression by Electroporation; title: Terence J. Van Raay and Michael R. Stark223; title: 21 Gene Therapy Using Neural Stem Cells; title: Luciano Conti and Elena Cattaneo 233; title: 22 Modeling Brain Pathologies Using Neural Stem Cells; title: Simonetta Siplone and Elena Cattaneo245; title: PART III UTILIZATION/CHARACTERIZATION OF NSCs IN VivO; title: A. ENDOGENOUS POOLS OF STEM/PROGENITOR CELLS; title: 23 Activation and Differentiation of Endogenous Neural Stem Cell Progeny in the Rat Parkinson Animal Model; title: Marcel M. Daadi 265; title: 24 Identification of Musashil-Positive Cells in Human Normal and Neoplastic Neuroepithelial Tissues by Immunohistochemical Methods; title: Yonehiro Kanemura, Shin-ichi Sakakibara,; title: and Hideyuki Okano273; title: 25 Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo; title: Sanjay S. P. Magavi and Jeffrey D. Macklis283; title: 26 Immunocytochemical Analysis of Neuronal Differentiation; title: Sanjay S. P. Magavi and Jeffrey D. Macklis291; title: 27 Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold; title: Lisa A. Catapano, Sanjay S. P. Magavi,; title: and Jeffrey D. Macklis299; title: B. TRANSPLANTATION; title: 28 Labeling Stem Cells In Vitro for Identification of Their Differentiated Phenotypes After Grafting into the CNS; title: Qi-lin Cao, Stephen M. Onifer, and Scott R. Whittemore307; title: 29 Optimizing Stem Cell Grafting into the CNS; title: Scott R. Whittemore, Y. Ping Zhang, Christopher B. Shields,; title: Dante J. Morassutti, and David S. K. Magnuson319; title: 30 Vision-Guided Technique for Cell Transplantation and Injection of Active Molecules into Rat and Mouse Embryos; title: Lorenzo M agrassi327; title: 31 Transplantation into Neonatal Rat Brain as a Tool to Study Properties of Stem Cells; title: Tanja Zigova and Mary B. Newman341; title: 32 Routes of Stem Cell Administration in the Adult Rodent; title: Alison E. Willing, Svitlana Garbuzova-Davis,; title: Paul R. Sanberg, and Samuel Saporta357; title: Index3 7 5.