Viral vectors for gene therapy
Subtitle: methods and protocols
By: edited by Otto-Wilhelm Merten, Mohamed Al-Rubeai
Series: Methods in molecular biology -- v. 737; Methods in molecular biology (Clifton, N.J.) -- v. 737.
Publisher: Humana Press
Published: 2011
Place of publication: New York
Language: eng
Number of pages: 450
ISBN-13: 9781617790942; 9781617790959
Subjects: Transfection; Gene therapy; Genetic vectors; Viral genetics; Virus-vector relationships
Notes: Includes bibliographical references and index.
Table of contents: title: Introduction to viral vectors; title: Introduction to gene therapy: A clinical aftermath; title: Host cells and cell banking; title: Overview of current scalable methods for purification of viral vectors; title: Methods to construct recombinant adenovirus vectors; title: Manufacturing of adenovirus vectors: Production and purification of helper dependent adenovirus; title: Manufacturing of retroviruses; title: Lentiviral vectors; title: Adeno-associated viruses; title: Manufacturing of adeno-associated viruses, for example: AAV2; title: Vector characterization methods for quality control testing of recombinant adeno-associated viruses; title: Baculoviruses mediate efficient gene expression in a wide range of vertebrate cells; title: Herpes simplex virus type 1-derived recombinant and amplicon vectors; title: Manufacture of measles viruses; title: In vivo gene delivery into hCD34+ cells in a humanized mouse model; title: In vivo evaluation of gene transfer into mesenchymal cells (in view of cartilage repair); title: Ethical consideration; title: Clinical trials of GMP products in the gene therapy field.