Laser Capture Microdissection
Subtitle: Methods and Protocols (Methods in Molecular Biology)
Contributors: Graeme I. Murray (Editor); Stephanie Curran (Editor)
Edition: 1 edition
Publisher: Humana Press
Published: December 23, 2004
Language: eng
Format: Hardcover
Number of pages: 310
ISBN-13: 9781588292605
Subjects: Molecular biology; Life Sciences - Biology - Molecular Biology; Science; Science/Mathematics; diagnostic use; Life Sciences - Biochemistry; Science / Cytology; Lasers; Methodology; Microdissection
First sentence: Tissues, especially diseased tissues, are complex three-dimensional structures composed of heterogeneous mixtures of morphologically and phenotypically distinct cell types.
Description: Describes in step-by-step detail how to successfully carry out tissue-based laser microdissection in the laboratory using the different systems available and to apply a wide range of sophisticated molecular technologies to the microdissected tissue samples. Highlights include gene expression profiling of primary tumor cell populations using laser capture microdissection, RNA transcript amplification and GeneChip microarrays, laser capture microdissection and colorectal cancer proteomics, and proteomic analysis of human bladder tissue using SELDI approach following microdissection techniques. Applications of the microdissected tissue described encompass PCR, gene expression studies including microarray analysis, and proteomics.