Parasite genomics protocols
By: edited by Sara E. Melville
Contributors: Melville, Sara E
Series: Methods in molecular biology -- v. 270; Methods in molecular biology (Clifton, N.J.) --  270
Publisher: Humana Press
Published: 2004
Place of publication: Totowa, N.J
Language: eng
Number of pages: 452
Subjects: Parasites -- Molecular genetics -- Methodology; Genomics -- Laboratory manuals
Genres: Laboratory manuals
Notes: Includes bibliographical references and index
Table of contents: title: Sequencing strategies for parasite genomes / Daniella Bartholomeu and Najib M. El-Sayed; title: Annotation of parasite genomes / Matthew Berriman and Midori Harris; title: Parasite genome databases and web-based resources / Christiane Hertz-Fowler and Neil Hall; title: Expressed sequence tags: medium throughput protocols / Claire Whitton, Jennifer Daub, Marian Thompson, and Mark Blaxter; title: Expressed sequence tags: analysis and annotation / John Parkinson and Mark Blaxter; title: Positive selection scanning of parasite DNA sequences; title: Winston A. Hide and Raphael D. Isokpehi; title: Race and rage cloning in parasitic microbial eukaryotes / Bryony A. P. Williams and Robert P. Hirt; title: Amplified (restriction) fragment length polymorphism (AFLP) analysis / Daniel K. Masiga and C. Michael R. Turner; title: Minisatellites and MVR-PCR for the individual identification of parasite isolates / Annette MacLeod; title: Analysis of differentially expressed parasite genes and proteins using transcriptomics and proteomics / Daniel C. Gare; title: Gene expression studies using self-fabricated parasite cDNA microarrays / Karl F. Hoffmann and Jennifer M. Fitzpatrick; title: DNA content analysis on microarrays / Upinder Singh, Preetam H. Shah, and Ryan C. MacFarlane; title: Typing single nucleotide polymorphisms in toxoplasma gondii by allele-specific primer extension and microarray detection / Chunlei Su, Christian Hott, Bernard H. Brownstein, and L. David Sibley; title: Transfection of the human malaria parasite plasmodium falciparum / Brendan S. Crabb, Melanie Rug, Tim-Wolf Gilberger, Jennifer K. Thompson, Tony Triglia, Alexander G. Maier, and Alan F. Cowman; title: A PCR-based method for gene deletion and protein tagging in trypanosoma brucei / George K. Arhin, Shuiyuan Shen, Elisabetta Ullu, and Christian Tschudi; title: Analysis of gene function in trypanosoma brucei using RNA interference / Appolinaire Djikeng, Shuiyuan Shen, Christian Tschudi, and Elisabetta Ullu; title: In vitro shuttle mutagenesis using engineered mariner transposons / Kelly A. Robinson, Sophie Goyard, and Stephen M. Beverley; title: Random mutagenesis strategies for construction of large and diverse clone libraries of mutated DNA fragments / Sudsanguan Chusacultanachai and Yongyuth Yuthavong; title: Separation, digestion and cloning of intact parasite chromosomes embedded in agarose / Vanessa Leech, Michael A. Quail, and Sara E. Melville; title: Chromosome fragmentation in leishmania / Pascal Dubessay, Christine Blaineau, Patrick Bastien, and Michel PagÅs; title: FISH mapping for helminth genomes / Hirohisa Hirai and Yuriko Hirai; title: Fiber-FISH: fluorescence in situ hybridization on stretched DNA / Klaus Ersfeld; title: Yeast two-hybrid assay for studying protein-protein interactions / Ahmed Osman; title: From genomes to vaccines for leishmaniasis / Carmel B. Stober