Guide to techniques in mouse development
Subtitle: Mouse molecular genetics
By: edited by Paul M. Wassarman and Philippe M. Soriano
Contributors: ScienceDirect (Online service)
Edition: 1st ed.
Series: Methods of enzymology -- v. 477
Publisher: Elsevier /Academic Press
Published: 2010
Place of publication: Amsterdam; Boston
Language: eng
Format: [electronic resource] /
ISBN-13: 9780123848819; 9780123848826; 9780123848802
Subjects: Development; Life Sciences; Cytology; Cell Biology; SCIENCE; Medicine; Mice; Embryology
Other titles: Mouse molecular genetics
Description: This volume�comprehensively covers�new technologies�and methodologies that have appeared for the study of mouse development.�� This volume is Part B�of an update�of volume 225, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993.� Comprehensively covers�new techniques for the cryopreservation of gametes and embryos, production of transgenic and null (knockout) animals (use of ES cells), generation of conditional/inducible mutant animals, use of gene-trap
Notes: Originally published in one volume: Guide to techniques in mouse development / Paul M. Wassarman, Melvin L. DePamphilis. San Diego : Academic Press, c1993.

Includes bibliographical references and indexes.

Description based on print version record.
Table of contents: title: Part B. Lentivirus transgenesis; title: Lentivirus transgenesis; title: Germline modification using mouse spermatogonial stem cells; title: Embryonic in vivo electroporation in the mouse; title: Current applications of transposons in mouse genetics; title: Functional genomics in the mouse using the sleeping beauty transposon system; title: The use of DNA transposons for cancer gene discovery in mice; title: A practical summary of site-specific recombination, conditional mutagenesis, and tamoxifen induction of creert2; title: A recombineering pipeline to make conditional targeting constructs; title: Confirmation of recombination site functionality in gene targeting vectors using recombinase-expressing bacteria; title: Genetic fate mapping using site-specific recombinases; title: Mapping cell fate and function using recombinase-based intersectional strategies; title: Genome-wide forward genetic screens in mouse es cells; title: Gene trap mutagenesis in the mouse; title: A wider context for gene trap mutagenesis; title: Mouse mutagenesis with the chemical supermutagen enu; title: Phenotype-driven mouse enu mutagenesis screens; title: Using enu mutagenesis for phenotype-driven analysis of the mouse; title: Exploration of self-renewal and pluripotency in es cells using rnai; title: Transgenic rnai applications in the mouse; title: Gene knockdown in the mouse through rnai; title: In vivo analysis of gene knockdown in tetracycline-inducible shrna mice; title: The power of reversibility regulating gene activities via tetracycline-controlled transcription; title: Gene expression profiling of mouse oocytes and preimplantation embryos; title: Interrogating the transcriptome of oocytes and preimplantation embryos; title: Gene expression profiling of mouse embryos with microarrays; title: Technological advances in mouse development studies.