cAMP signaling
Subtitle: methods and protocols
By: edited by Manuela Zaccolo
Series: Methods in molecular biology -- 1294; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 1294,; Springer protocols (Series)
Publisher: Humana Press
Published: 2015
Place of publication: New York, NY
Language: eng
Number of pages: 222
ISBN-13: 9781493925360; 9781493925377
Subjects: Laboratory Manuals; Cyclic AMP; Fluorescence Resonance Energy Transfer; Signal Transduction; A Kinase Anchor Proteins; Cyclic AMP-Dependent Protein Kinases; Cyclic adenylic acid; Laboratory manuals
Notes: Includes bibliographical references and index.
Table of contents: title: Simultaneous assessment of cAMP signaling events in different cellular compartments using FRET-based reporters  -- Alex Burdyga and Konstantinos Lefkimmiatis; title: Recording intracellular cAMP levels with EPAC-based FRET sensors by fluorescence lifetime imaging  -- Marcel Raspe, Jeffrey Klarenbeek, and Kees Jalink; title: Novel approach combining real-time imaging and the patch-clamp technique to calibrate FRET-based reporters for cAMP in their cellular microenvironment  -- Andreas Koschinski and Manuela Zaccolo; title: Structure-based, in silico approaches for the development of novel cAMP FRET reporters  -- Matías Machado and Sergio Pantano; title: Automated image analysis of FRET signals for subcellular cAMP quantification  -- Silas J. Leavesley [and others]; title: Channel-based reporters for cAMP detection  -- Thomas C. Rich [and others]; title: Imaging sub-plasma membrane cAMP dynamics with fluorescent translocation reporters  -- Anders Tengholm and Olof Idevall-Hagren; title: Adenoviral transduction of FRET-based biosensors for cAMP in primary adult mouse cardiomyocytes  -- Oliver Lomas [and others]; title: Generation of transgenic mice expressing FRET biosensors  -- Daniela Hübscher and Viacheslav O. Nikolaev; title: Photoactivatable adenylyl cyclases (PACs) as a tool to study cAMP signaling in vivo : an overview  -- Marina Efetova and Martin Schwärzel; title: Selective disruption of the AKAP signaling complexes  -- Eileen J. Kennedy and John D. Scott; title: Screening for small molecule disruptors of AKAP-PKA interactions  -- Carolin Schächterle [and others]; title: Structure-based bacteriophage screening for AKAP- selective PKA regulatory subunit variants  -- Ryan Walker-Gray and Matthew G. Gold; title: Yeast-based high-throughput screen for modulators of phosphodiesterase activity  -- Ana Santos de Medeiros and Charles S. Hoffman; title: Separation of PKA and PKG signaling nodes by chemical proteomics  -- Eleonora Corradini, Albert J.R. Heck, and Arjen Scholten; title: Development of computational models of cAMP signaling -- Susana R. Neves-Zaph and Roy S. Song.