The surprising reactivity patterns of intermediates derived from thiamin and benzoylformate
By: by Qingyan Hu.
Published: 2006
Language: eng
Number of pages: 109
ISBN-13: 9780494157503
Description: The conversion of benzoylformate to benzaldehyde and carbon dioxide is catalyzed by benzoylformate decarboxylase (BFD), an enzyme that utilizes thiamin diphosphate (TDP) as a cofactor. There is no reaction at all in the absence of the enzyme.*We synthesized alpha-mandelyl-thiamin (MT), a species whose inherent reactivity should be very close to that of the intermediate formed on the enzyme. This precisely separates the role of the protein and cofactor in catalysis. We identified several important distinctions: (1) Decarboxylation of MT produces 2-1-(hydroxybenzyl)thiamin (HBnT) in competition with fragmentation products (that destroy thiamin). The enzyme avoids the fragmentation process. (2) The decarboxylation of MT is slower (by about 106) than kcat of BFD. The protein accelerates what appears to be a unimolecular process. (3) Fragmentation of the decarboxylation product of MT is several orders of magnitude faster than k cat of BFD. The enzyme avoids the faster process. A likely mechanism would be the result of stereoelectronic effects. (4) Protonation of the pyrimidine of MT, as modeled by N1'-methylation, has no effect on the competition between fragmentation and protonation products. Therefore, charge on the pyrimidine does not control product distribution. (5) Bronsted acid catalysts in general promote formation of HBnT over fragmentation, but do not accelerate decarboxylation. However, protonated pyridines accelerate the decarboxylation. Since there is no role for an acid in breaking the C-C bond, it is likely that the acid is trapping the intermediate in competition with a reverse reaction (CO2 addition).Thus, the reactions of the intermediates are clearly controlled by the protein. Based on the results in the thesis, we conclude that primary function of TDP is to serve as a point of covalent attachment, electrostatic stabilization of transition states, and specific orientation within the protein that controls reactivity and access to protein side chain catalytic groups.The likely mechanism involves initial addition of the cofactor to enzyme-bound benzoylformate to produce alpha-mandelyl-TDP as an intermediate, from which CO2 is lost to generate 2-(1-hydroxybenzyl)-TDP, which then releases benzaldehyde and TDP.**Please refer to dissertation for diagrams.
Notes: Source: Dissertation Abstracts International, Volume: 67-06, Section: B, page: 3138.

Advisor: Ronald Kluger

Thesis (Ph.D.)--University of Toronto, 2006.

Includes bibliographic references.

Electronic version licensed for access by U. of T. users.