Protein Folding Kinetics
Subtitle: Biophysical Methods
By: by Bengt Nölting
Publisher: Springer Berlin Heidelberg
Published: 1999
Place of publication: Berlin, Heidelberg
Language: eng
Format: [electronic resource] :
Number of pages: 191
ISBN-13: 9783662039687; 9783662039663
Subjects: Biochemistry; Biotechnology; Life sciences; Food science
Description: The book imparts insight into the principles and concepts of the kinetic and structural resolution of fast chemical and biophysical reactions of proteins with emphasis on protein folding reactions. The study of fast protein folding reactions and the understanding of the folding paradox have advanced upon the recent development of new biophysical methods which enable not only kinetic resolution in the sub-millisecond time scale but also higher structural resolution. The pathways and structures of early folding events and the transition state structures of fast folding proteins can now be studied in far more detail. Important techniques include biophysical, chemical, molecular biological and mathematical methods, in particular protein engineering, F-value analysis, circular dichroism, fluorescence, pulsed LASER spectroscopy, temperature jumping, ultrafast mixing, and stopped flow.
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Table of contents: title: High kinetic resolution of protein folding events; title: High structural resolution of transient protein conformations; title: The folding pathway of a protein (barstar) at the resolution of individual residues from microseconds to seconds.