Viability and differentiation of smooth muscle progenitor cells on altered forms of collagen matrix.
Published: 2005
Language: eng
Number of pages: 128
Description: Cells isolated from murine bone marrow (BM) can be driven to become smooth muscle progenitor cells (SPC) that express smooth muscle-specific (SM) markers at levels equivalent to those found in primary aortic SM cell cultures. SM alpha-actin, SM22-alpha and SM-MHC mRNA expression was 2386, 312 and 1.2-fold greater than BM at 10d, and 5143, 573, and 13.8-fold greater than BM at 21d, respectively. The presence of 50ng/mL PDGF-BB reversibly lowered SM marker mRNA and protein expression. Compared to fibrillar collagen (fCol), SPCs cultured on non-fibrillar collagen (mCol) exhibited 2.8-fold decreased SM alpha-actin expression by 72h, 5.3-fold increased apoptosis, and 4.3-fold decreased proliferation by 72h. SPCs on mCol were spindle-shaped, with disorganized cytoskeletal elements, whereas SPCs on fCol were flattened and polygonal with a mature cytoskeleton in a stress-fibre orientation. These results suggest that accumulation of PDGF-BB and non-fibrillar collagen in diseased vasculature may negatively affect the regenerative potential of SPCs.
Notes: Source: Masters Abstracts International, Volume: 44-02, page: 0959.

Thesis (M.A.Sc.)--University of Toronto, 2005.

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