The role of Shh-dependent Gli activator and repressor functions in epidermal development and disease.
Published: 2004
Language: eng
Number of pages: 238
Description: Tightly regulated Sonic hedgehog (Shh) signaling in the skin is required for epidermal growth during hair follicle development, but unregulated responses result in common human skin tumors. How Hh signals are mediated in target cells is determined by the combined activities of members of the Gli family of zinc finger transcription factors. The role of Gli transcription factors during skin development and disease was previously unknown. Using a genetic approach complementing loss-of-function and gain-of-function mouse mutants, we have been able to dissect temporal and spatial requirements for Shh signaling in the skin. During hair follicle morphogenesis, Shh signaling is primarily required by the epidermis to promote the proliferation of epidermal progenitors. Two important functions of Shh signaling are required for mitogenic responses to occur; (1) promoting the generation of Gli activators, especially Gli2ACT and (2) inhibiting the generation of Gli repressors, primarily Gli3REP. The summation of these Gli activities determines the transcriptional activity of target genes and ultimately, the decision to enter the cell cycle and proliferate. These studies have revealed novel Gli-dependent regulation of a transcriptional hierarchy of key cell cycle regulators, including the D-type cyclins, N-myc and  E2Fs. Furthermore, it has unveiled functional differences in how these target genes are regulated by Gli functions. In Shh;Gli3 double mutants, the expression of early cell cycle markers was derepressed, but induction of later regulators, namely activator E2Fs, could not be induced without GliACT, and no robust proliferative responses were observed. These results suggest that Shh signaling controls multiple levels of the cell cycle to ensure that proliferative responses are restricted in time and space to regions of high Hh signal. The Hh-dependent balance of Gli activator and repressor functions also plays a key role in limiting proliferative responses to adult hair follicles at the telogen-anagen transition. Disturbing this balance, either by increasing the magnitude of Hh responses transiently at anagen in K5Cre;Z/APGli2 mice or extending Hh responses independently of the hair cycle in K5Cre;Z/APDeltaNGli2  mice, is sufficient to overwhelm endogenous regulation. These changes disrupt epidermal homeostasis, triggering hyperproliferative responses and tumors in transgenic skin.
Notes: Adviser:  Chi-chung Hui.

Thesis (Ph.D.)--University of Toronto, 2004.

Electronic version licensed for access by U. of T. users.

Source: Dissertation Abstracts International, Volume: 65-10, Section: B, page: 4965.