MAP kinase signaling protocols
By: edited by Rony Seger
Edition: 2nd ed.
Series: Springer protocols; Methods in molecular biology -- v. 661
Publisher: Humana Press
Published: 2010
Place of publication: New York, N.Y
Language: eng
Number of pages: 529
ISBN-13: 9781607617945
Subjects: Mitogen-activated protein kinases; Methodology; Microbiological Techniques; Cellular signal transduction; MAP Kinase Signaling System; Research
Notes: Includes bibliographical references and index.
Table of contents: title: The MAP kinase signaling cascades : A system of hundreds of components regulates a diverse array of physiological functions; title: Determination of ERK activity: Anti-phospho-ERK antibodies and in vitro phosphorylation; title: Activation of SAPK/JNKs in vitro; title: Activation of p38 and determination of its activity; title: Activity assays for extracellular signal-regulated kinase 5; title: Use of inhibitors in the study of MAP kinases; title: MAP kinase activation by receptor tyrosine kinases : in control of cell migration; title: Activation of ras and rho GTPases and MAP kinases by G-protein-coupled receptors; title: Regulation of MAP kinase signaling by calcium; title: Identification of novel substrates of MAP kinase cascades using bioengineered kinases that uniquely utilize analogs of ATP to phosphorylate substrates; title: ERK-MAP kinase signaling in the cytoplasm; title: Lentiviral vectors to study the differential function of ERK1 and ERK2 MAP kinases; title: Structural studies of MAP kinase cascade components; title: ^; title: Analysis of MAP kinases by hydrogen exchange mass spectrometry; title: A "Molecular evolution" approach for isolation of intrinsically active (MEK-independent) MAP kinases; title: Reconstitution of the nuclear transport of the MAP kinase ERK2; title: Localization and trafficking of fluorescently tagged ERK1 and ERK2; title: Studying the regulation of MAP kinase by MAP kinase phosphatases in vitro and in cell systems; title: Proteomic analysis of scaffold proteins in the ERK cascade; title: Analysis of ERKs' dimerization by electrophoresis; title: MAP kinase: SUMO pathway interactions; title: Computational modelling of kinase signalling cascades; title: Analysis of mitogen-activated protein kinase activity in yeast; title: Detection of RTK pathway activation in drosophila using anti-dpERK immunofluorescence staining; title: Studying MAP kinase pathways during early development of xenopus laevis; title: Deciphering signaling pathways in vivo : The ras/raf/MEK/ERK cascade; title: ^; title: ^^; title: Mutational and functional analysis in human ras/MAP kinase genetic syndromes; title: Implication of the ERK pathway on the post-transcriptional regulation of VEGF mRNA stability; title: Studies on MAP kinase signaling in the immune system; title: Methods to study MAP kinase signalling in the central nervous system; title: MAP kinase regulation of the mitotic spindle checkpoint; title: Using high-content microscopy to study gonadotrophin-releasing hormone regulation of ERK.; title: ^^