Matrix metalloproteinase protocols
By: edited by Ian M. Clark ; consulting editors, David A. Young, Andrew D. Rowan
Edition: 2nd ed.
Series: Methods in molecular biology -- 622; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 622.; Springer protocols
Publisher: Humana Press
Published: 2010
Place of publication: New York, N.Y
Language: eng
Number of pages: 492
ISBN-13: 9781603272988
Notes: Previous ed.: 2001.

Includes bibliographical references and index.
Table of contents: title: Metalloproteases and the degradome / Alejandro P. Ugalde ... [et al.]; title: Mouse models of MMP and TIMP function / Sean E. Gill ... [et al.]; title: Expression of recombinant MMP-28 in mammalian cells / Ursula R. Rodgers and Ian M. Clark; title: Expression of recombinant matrix metalloproteinases in Escherichia coli / L. Jack Windsor and Darin L. Steele; title: Expression of recombinant ADAMTS in insect cells / Gavin C. Jones, Mireille N. Vankemmelbeke and David J. Buttle; title: Expression and purification of membrane-type MMPs / Jing Nie and Duanqing Pei; title: Refolding of TIMP-2 from Escherichia coli inclusion bodies / Richard A. Williamson; title: Purification of MMPs and TIMPs / Ken-ichi Shimokawa and Hideaki Nagase; title: Real-time PCR expression profiling of MMPs and TIMPs / Caroline J. Pennington and Dylan R. Edwards; title: Analysis of the degradome with the CLIP-CHIP microarray / Reinhild Kappelhoff, Ulrich auf dem Keller, and Christopher M. Overall; title: In situ hybridization for metalloproteinases and their inhibitors / Tiina L. Hurskainen and Suneel S. Apte; title: Immunohistochemistry of MMPs and TIMPs / Yasunori Okada; title: Single nucleotide polymorphism genotyping in MMP genes : the 5' nuclease assay / Ross Laxton and Shu Ye; title: Methods for studying activation of matrix metalloproteinases / Vera Knäuper and Gillian Murphy; title: Assay of matrix metalloproteinases against matrix substrates / Tim E. Cawston, Rachel L. Lakey, and Andrew D. Rowan; title: Zymography and reverse zymography for detecting MMPs and TIMPs / Susan P. Hawkes, Hongxia Li, and Gary T. Taniguchi, with contributions from Marc A. Lafleur; title: In situ zymography / Sarah J. George and Jason L. Johnson; title: Near-infrared optical proteolytic beacons for in vivo imaging of matrix metalloproteinase activity / J. Oliver McIntyre, Randy L. Scherer, and Lynn M. Matrisian; title: Neoepitope antibodies against MMP-cleaved and aggrecanase-cleaved aggrecan / Amanda J. Fosang ... [et al.]; title: In vitro model of cartilage degradation / Wang Hui and Tim E. Cawston; title: Immunoassays for collagenase-mediated cleavage of type I and II collagens / R. Clark Billinghurst, Mirela Ionescu, and A. Robin Poole; title: Collagen degradation assays / Anthony P. Hollander; title: Analysis of MMP-dependent cell migration and invasion / Ralf Palmisano and Yoshifumi Itoh; title: Using fluorogenic peptide substrates to assay matrix metalloproteinases / Gregg B. Fields; title: Kinetic analysis of the inhibition of matrix metalloproteinases : lessons from the study of tissue inhibitors of metalloproteinases / Frances Willenbrock, Daniel A. Thomas, and Augustin Amour; title: Identification of cellular MMP substrates using quantitative proteomics : isotope-coded affinity tags (ICAT) and isobaric tags for relative and absolute quantification (iTRAQ) / Georgina S. Butler ... [et al.]; title: Mechanism-based profiling of MMPs / Jed F. Fisher and Shahriar Mobashery.