In situ hybridization protocols
By: edited by Ian A. Darby, Tim D. Hewitson.
Contributors: Darby, Ian A.; Hewitson, Tim D.
Edition: 3rd ed.
Series: Methods in molecular biology,; v. 326; Methods in molecular biology (Clifton, N.J.);; v. 326.
Publisher: Humana Press
Published: 2006
Place of publication: Totowa, N.J
Language: eng
Number of pages: 269
Subjects: In situ hybridization -- Laboratory manuals; In Situ Hybridization -- Laboratory Manuals
Genres: Laboratory Manuals.
Description: This revised and updated edition emphasizes tissue and cell in situ hybridization methods. Among the new techniques detailed are PNA probes for viral diagnostics, plant in situ hybridization, cell proliferation detection, and quantitation of in situ hybridization. There are also cutting-edge techniques for tissue microarrays, expanded embryology-developmental gene detection, and expanded cell culture. Derivative techniques presented include identification of transplanted cells, histones, nick-end labeling for apoptosis, the use of peptide nucleic acid probes, and in situ hybridization of plant specimens.
Notes: Includes bibliographical references and index.
Table of contents: title: Treatment of tissue sections for in situ hybridization / Gregory H. Tesch, Hui Y. Lan, and David J. Nikolic-Paterson; title: Probe design / Peter Roche; title: cRNA probes: comparison of isotopic and nonisotopic detection methods / Teresa Bisucci, Tim D. Hewitson, and Ian A. Darby; title: Tyramide signal amplification for DNA and mRNA in situ hybridization / Ernst J.M. Speel, Anton H.N. Hopman, and Paul Komminoth; title: Expression analysis of murine genes using in situ hybridization with radioactive and non-radioactively labeled probes / Anne Chotteau-Lelièvre, Pascal Dollé, and Françoise Gofflot; title: Non-radioactive in situ hybridization on frozen sections and whole mounts / Julia Asp, Alexandra Abramsson, and Christer Betsholtz; title: In situ hybridization of whole mount embryos / Murray Hargrave, Josephine Bowles, and Peter Koopman; title: In situ detection of Epstein-Barr virus and phenotype determination of EBV-infected cells / Gerald Niedobitek and Hermann Herbst; title: PNA- situ hybridization method for detection of HIV-1 DNA in virus-infected cells and subsequent detection of cellular and viral proteins / Tomoko Hagiwara, Junko Hattori, and Tsuguhiro Kaneda; title: Electron microscopy and in situ hybridization: expression of P2Y2 receptor mRNA in the cerebellum / Andrzej Loesch and Rainer Glass; title: Free-floating sections of brain in situ / Neil Owens; title: An in situ hybridization technique to detect low-abundance slug mRNA in adherent cultured cells / Changsun Choi ... [et al.]; title: Identification of transplanted human cells in animal tissues / Daniel Benten, Kang Cheng, and Sanjeev Gupta; title: In situ hybridisation to plant tissues and chromosomes / Andreas Houben, Sharon J. Orford, and Jeremy N. Timmis; title: Histochemical localization of cell proliferation using in situ hybridization for histone mRNA / Tim D. Hewitson, Kristen J. Kelynack, and Ian A. Darby; title: Histochemical localization of apoptosis with in situ labeling of fragmented DNA / Tim D. Hewitson, Teresa Bisucci, and Ian A. Darby; title: The use of combined immunohistochemical labeling and in situ hybridization to co-localize mRNA and protein in tissue sections / Malcolm D. Smith, Michael Ahern, and Mark Coleman; title: Semi-quantitative in situ hybridization using radioactive probes to study gene expression in motoneuron populations / Paul D. Storer and Tracey DeLucia; title: Quantitative in situ hybridization of tissue microarrays / Adrian M. Jubb ... [et al.].