Small non-coding RNAs
Subtitle: methods and protocols
By: edited by Mathieu Rederstorff
Series: Methods in molecular biology -- 1296; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 1296.; Springer protocols (Series)
Publisher: Humana Press
Published: 2015
Place of publication: New York
Language: eng
Number of pages: 238
ISBN-13: 9781493925469; 9781493925476
Subjects: Genetics; Methods; Non-coding RNA; Laboratory Manuals; Small Untranslated RNA; RNA Sequence Analysis; Laboratory manuals; Physiology
Notes: Includes bibliographical references and index.
Table of contents: title: Small non-coding RNAs : a quick look in the rearview mirror  -- Guillaume Clerget, Yoann Abel, and Mathieu Rederstorff; title: Alcoholic precipitation of small non-coding RNAs  -- Guillaume Clerget, Valérie Bourguignon-Igel, and Mathieu Rederstorff; title: Quantification and quality control of a small non-coding RNA preparation  -- Virginie Marchand and Christiane Branlant; title: Impact of RNA isolation protocols on RNA detection by Northern blotting  -- Katrin Damm [and seven others]; title: Improved Northern blot detection of small RNAs using EDC crosslinking and DNA/LNA probes  -- Katrin Damm [and seven others]; title: Direct cloning of double-stranded RNAs  -- Manli Shen [and three others]; title: Detection and labeling of small non-coding RNAs by splinted ligation  -- Gabrielle Bourgeois [and three others]; title: Fluorescence in situ hybridization of small non-coding RNAs  -- Valentin Vautrot [and three others]; title: RT-qPCR-based quantification of small non-coding RNAs  -- Fjoralba Zeka, Pieter Mestdagh, and Jo Vandesompele; title: Stem-loop RT-PCR based quantification of small non-coding RNAs  -- Véronique Salone and Mathieu Rederstorff; title: miR-RACE : an effective approach to accurately determine the sequence of computationally identified miRNAs  -- Chen Wang and Jinggui Fang; title: Probing small non-coding RNAs structures  -- Jean-Vincent Philippe [and three others]; title: cDNA library generation for the analysis of small RNAs by high-throughput sequencing  -- Jennifer Gebetsberger, Roger Fricker, and Norbert Polacek; title: CLIP-Seq to discover transcriptome-wide imprinting of RNA binding proteins in living cells  -- Jérôme Saulière and Hervé Le Hir; title: Microarray analysis of small non-coding RNAs  -- Michael Karbiener and Marcel Scheideler; title: RML-RACE, PPM-RACE, and qRT-PCR : an integrated strategy to accurately validate miRNA target genes  -- Chen Wang and Jinggui Fang; title: Dual luciferase gene reporter assays to study miRNA function  -- Thomas Clément, Véronique Salone, and Mathieu Rederstorff; title: Gene expression knockdown by transfection of siRNAs into mammalian cells  -- Yoann Abel and Mathieu Rederstorff; title: Efficient and selective knockdown of small non-coding RNAs  -- Xue-Hai Liang, Wen Shen, and Stanley T. Crooke; title: Cell-SELEX : in vitro selection of synthetic small specific ligands  -- Helena Dickinson [and three others]; title: Small non-coding RNAs and aptamers in diagnostics and therapeutics -- Marissa Leonard, Yijuan Zhang, and Xiaoting Zhang.