Mitosis
Subtitle: methods and protocols
By: edited by Andrew D. McAinsh
Series: Methods in molecular biology -- 545; Methods in molecular biology (Clifton, N.J.) -- v. 545.
Publisher: Humana Press
Published: 2009
Place of publication: Dordrecht; New York
Language: eng
Number of pages: 345
ISBN-13: 9781603279925; 9781603279932
Notes: Includes bibliographical references and index.
Table of contents: title: Knock-in and knock-out: the use of reverse genetics in somatic cells to dissect mitotic pathways / A.R. Barr, D. Zyss and F. Gergely; title: Functional dissection of mitotic regulators through gene targeting in human somatic cells / E. Berdougo, M.E. Terret and P.V. Jallepalli; title: RNAi in Drosophila S2 cells as a tool for studying cell cycle progression / M. Bettencourt-Dias and G. Goshima; title: Production of mitotic regulators using an autoselection system for protein expression in budding yeast / M. Geymonat, A. Spanos and S. Sedgwick; title: Hydrodynamic analysis of human kinetochore complexes during mitosis / S.E. McClelland and A.D. McAinsh; title: Isolation of protein complexes involved in mitosis and cytokinesis from Drosophila cultured cells / P.P. D'Avino ... [et al.]; title: Automated live microscopy to study mitotic gene function in fluorescent reporter cell lines / M.H. Schmitz and D.W. Gerlich; title: Electron tomography of microtubule end-morphologies in C. elegans embryos / E. O'Toole and T. Muller-Reichert; title: Dissecting mitosis with laser microsurgery and RNAi in Drosophila cells / A.J. Pereira ... [et al.]; title: Fluorescence imaging of the centrosome cycle in mammalian cells / S.L. Prosser and A.M. Fry; title: Visualization of fluorescence-tagged proteins in fission yeast: the analysis of mitotic spindle dynamics using GFP-tubulin under the native promoter / M. Sato, M. Toya and T. Toda; title: Analysing kinetochore function in human cells: spindle checkpoint and chromosome congression / C. Klebig ... [et al.]; title: Probing kinetochore structure and function using Xenopus laevis frog egg extracts / M.J. Emanuele and P.T. Stukenberg; title: Live cell imaging of kinetochore capture by microtubules in budding yeast / K. Tanaka and T.U. Tanaka; title: The spindle checkpoint: assays for the analysis of spindle checkpoint arrest and recovery / J. Fernius and K.G. Hardwick; title: Measuring proteolysis in mitosis / C. Lindon and B.D. Fiore; title: An in vitro assay for Cdc20-dependent mitotic anaphase-promoting complex activity from budding yeast / S.C. Schuyler and A.W. Murray; title: In vitro assays for the anaphase-promoting complex/cyclosome (APC/C) in Xenopus egg extracts / H. Yamano ... [et al.]; title: Preparation of synchronized human cell extracts to study ubiquitination and degradation / A. Williamson, L. Jin and M. Rape; title: Biochemical analysis of the anaphase promoting complex: activities of E2 enzymes and substrate competitive (pseudosubstrate) inhibitors / M.K. Summers and P.K. Jackson.