Methods in yeast genetics
Subtitle: a Cold Spring Harbor Laboratory course manual
By: David C. Amberg, Daniel J. Burke, Jeffrey N. Strathern
Contributors: Amberg, David C; Burke, Dan, 1954-; Strathern, Jeffrey N; Burke, Dan, 1954-; Cold Spring Harbor Laboratory
Edition: 2005 ed
Publisher: Cold Spring Harbor Laboratory Press
Published: 2005
Place of publication: Cold Spring Harbor, N.Y
Language: eng
Number of pages: 230
Subjects: Yeast fungi -- Genetics -- Laboratory manuals
Genres: Laboratory manuals
Other titles: Yeast genetics
Notes: Rev. ed. of: Methods in yeast genetics / Dan Burke, Dean Dawson, Tim Stearns, 2000 ed. 2000

Includes bibliographical references
Table of contents: title: Looking at yeast cells; title: Isolation and characterization of auxotrophic, temperature-sensitive, and osmotic-sensitive mutants; title: Meiotic mapping; title: Mitotic recombination and random spore analysis; title: Transformation of yeast; title: Synthetic lethal mutants; title: Gene replacement; title: Isolation of ras2 suppressors; title: Manipulating cell types; title: Isolating mutants by insertional mutagenesis; title: Isolation of separation of function mutants by two-hybrid differential interaction screening; title: High-efficiency transformation of yeast; title: "Quick and dirty" plasmid transformation of yeast colonies; title: Yeast DNA isolations; title: Yeast protein extracts; title: TAP purification; title: Yeast RNA isolations; title: Hydroxylamine mutagenesis of plasmid DNA; title: Assay of beta-galactosidase in yeast; title: Plate assay for carboxypeptidase Y; title: Random spore analysis; title: Yeast vital stains; title: Yeast immunofluorescence; title: Actin staining in fixed cells; title: PCR-mediated gene disruption; title: Yeast colony PCR; title: Measuring yeast cell density by spectrophotometry; title: Cell synchrony; title: Chromatin immunoprecipitation; title: Flow cytometry of yeast DNA; title: Logarithmic growth; title: EMS mutagenesis; title: Tetrad dissection; title: Making a tetrad dissection needle; title: Picking zygotes; title: Determining plating efficiency; title: DNA miniprep from e. coli; title: Preparing and transforming competent e. coli; title: Storing and handling the systematic deletion collection; title: Media; title: Stock preservation; title: Yeast genetic and physical maps; title: Templates for making streak plates; title: Electrophoretic karyotypes of strains for southern blot mapping; title: Strains; title: Counting yeast cells with a standard hemocytometerchamber; title: Tetrad scoring sheet