Human papillomaviruses
Subtitle: methods and protocols
By: edited by Clare Davy, John Doorbar.
Contributors: Davy, Clare.; Doorbar, John.
Series: Methods in molecular medicine -- 119
Publisher: Humana Press
Published: 2005
Place of publication: Totowa, N.J
Language: eng
Number of pages: 496
Subjects: Papillomavirus diseases -- Laboratory manuals.; Papillomaviruses -- Laboratory manuals.; Papillomavirus, Human -- genetics -- Laboratory Manuals.; Papillomavirus, Human -- pathogenicity -- Laboratory Manuals.; Cervix Neoplasms -- virology -- Laboratory Manuals.
Genres: Laboratory manuals.; Laboratory Manuals.
Notes: Includes bibliographical references and index.
Table of contents: value: Identification of new papillomavirus types / Ethel-Michele de Villiers, Corinna Whitley, and Karin Gunst; value: Identification of HPV variants / John Cason, Jon Bible, and Christine Mant; value: Histochemical analysis of cutaneous HPV-associated lesions / Kiyofumi Egawa; value: Histochemical analysis of cervical intraepithelial neoplasia / Michael Babawale ... [et al.]; value: Detection of papillomavirus proteins and DNA in paraffin-embedded tissue sections / Woei Ling Peh and John Doorbar; value: Detection and quantitation of HPV gene expression using real-time PCR / Rashmi Seth ... [et al.]; value: Analysis of P16INK4A and integrated genomes as progression markers / Svetlana Vinokurova, Nicolas Wentzensen, and Magnus von Knebel Doeberitz; value: Use of biomarkers in the evaluation of CIN grade and progression of early CIN / Jan P.A. Baak and Arnold-Jan Kruse; value: HPV DNA detection and typing in cervical scrapes / Peter J. F. Snijders ... [et al.]; value: HPV DNA detection and typing in inapparent cutaneous infections and premalignant lesions / Maurits de Koning ... [et al.]; value: Establishing HPV-containing keratinocyte cell lines from tissue biopsies / Margaret Anne Stanley; value: Using an immortalized cell line to study the HPV lifecycle in organotypic "raft" cultures / Paul F. Lambert ... [et al.]; value: Differentiation of HPV-containing cells using organotypic "raft" culture or methylcellulose / Regina Wilson and Laimonis A. Laimins; value: Propagation of infectious, high-risk HPV in organotypic "raft" culture / Margaret E. McLaughlin-Drubin and Craig Meyers; value: Retrovirus-mediated gene transfer to analyze HPV gene regulation and protein functions in organotypic "raft" cultures / N. Sanjib Banerjee, Louise T. Chow, and Thomas R. Broker; value: The HPV xenograft severe combined immunodeficiency (scid) mouse model / William Bonner; value: The cottontail rabbit papillomavirus model of high-risk HPV-induced disease / Janet L. Brandsma; value: Studying the HPV lifecycle in 3A trophoblasts and resulting pathophysiology / Yong Liu, Hong You, and Paul L. Hermonat; value: Replication and encapsidation of papillomaviruses in saccharomyces cerevisiae / Peter C. Angeletti; value: Analysis of the regulation of viral transcription / Bernd Gloss, Mina Kalantari, and Hans-Ulrich Bernard; value: Analysis of HPV transcripts by RPA / Jason M. Bodily and Craig Meyers; value: Analysis of regulatory motifs within HPV transcripts / Sarah A. Cumming and Sheila V. Graham; value: Detection of HPV transcripts by nested RT-PCR / Christine Mant, Barbara Kell, and John Cason; value: Analysis of HPV DNA replication using transient transfection and cell-free assays / Bing Yuan Lin, Thomas R. Broker, and Louise T. Chow; value: Detection and quantitation of HPV DNA replication by southern blotting and real-time PCR / Iain M. Morgan and Ewan R. Taylor; value: Analysis of E7/RB associations / Sandra Caldeira, Wen Dong, and Massimo Tommasino; value: Transformation assays for HPV oncoproteins / Paola Massimi and Lawrence Banks; value: Analysis of adeno-associated virus and hpv interaction / Paul L. Hermonat ... [et al.]; value: In vitro assays of substrate degradation induced by high-risk HPV E6 oncoproteins / Miranda Thomas and Lawrence Banks; value: Measuring the induction or inhibition of apoptosis by HPV proteins / Anna M. Kowalczyk ... [et al.]; value: Codon optimization of papillomavirus genes / Martin Muller; value: Generation of HPV pseudovirions using transfection and their use in neutralization assays / Christopher B. Buck ... [et al.]; value: Generation and application of HPV pseudovirions using vaccinia virus / Martin Sapp and Hans-Christoph Selinka.