Plant fungal pathogens
Subtitle: methods and protocols
By: edited by Melvin D. Bolton, Bart P.H.J. Thomma
Series: Methods in molecular biology -- 835; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 835.; Springer protocols
Publisher: Humana Press
Published: 2012
Place of publication: New York
Language: eng
Number of pages: 648
ISBN-13: 9781617795008; 9781617795015
Subjects: Fungal diseases of plants; Plant-pathogen relationships
Notes: Includes bibliographical references and index.
Table of contents: title: Fungal ABC transporter deletion and localization analysis; title: Targeted gene replacement in fungal pathogens via agrobacterium tumefaciens- mediated transformation; title: Activity-based protein profiling of infected plants; title: The use of agroinfiltration for transient expression of plant resistance and fungal effector proteins in nicotiana benthamiana leaves; title: Proteomic techniques for plant-fungal interactions; title: Identification of HR-inducing cDNAs from plant pathogens via a Gateway((r))-compatible binary potato virus X-expression vector; title: Freeze-fracture of infected plant leaves in ethanol for scanning electron microscopic study of fungal pathogens; title: Real-time PCR for the quantification of fungi in planta; title: Using non-homologous end-joining-deficient strains for functional gene analyses in filamentous fungi; title: Atomic force microscopy: A tool for studying biophysical surface properties underpinning fungal interactions with plants and substrates; title: Use of the yeast two-hybrid system to identify targets of fungal effectors; title: Characterization of plant-fungal interactions involving necrotrophic effector-producing plant pathogens; title: Heterologous production of fungal effectors in pichia pastoris; title: The application of laser microdissection to profiling fungal pathogen gene expression in planta; title: Metabolomics protocols for filamentous fungi; title: Targeted gene replacement in fungi using a split-marker approach; title: Massively parallel sequencing technology in pathogenic microbes; title: Confocal microscopy in plant-pathogen interactions; title: The use of open source bioinformatics tools to dissect transcriptomic data; title: Population biology of fungal plant pathogens; title: Polyethylene glycol (PEG)-mediated transformation in filamentous fungal pathogens; title: In vitro induction of infection-related hyphal structures in plant pathogenic fungi; title: Fungicide resistance assays for fungal plant pathogens; title: Identification of lipid-binding effectors; title: In silico identification and characterization of effector catalogs; title: Horizontal transfer of supernumerary chromosomes in fungi; title: The induction of mycotoxins by trichothecene producing fusarium species; title: Identifying genes in fusarium verticillioides through forward and reverse genetics; title: Assessment of autophagosome formation by transmission electron microscopy; title: Fungal plant pathogen detection in plant and soil samples using DNA macroarrays; title: Random insertional mutagenesis in fungal genomes to identify virulence factors; title: A yeast secretion trap assay for identification of secreted proteins from eukaryotic phytopathogens and their plant hosts; title: Comparing fungal genomes: Insight into functional and evolutionary processes; title: Multigene phylogenetic analyses to delimit new species in fungal plant pathogens; title: MAP kinase phosphorylation and cAMP assessment in fungi; title: A one-step affinity-purification protocol to purify NB-LRR immune receptors from plants that mediate resistance to fungal pathogens; title: Karyotyping methods for fungi; title: Isolation of apoplastic fluid from leaf tissue by the vacuum infiltration-centrifugation technique; title: Gene cloning using degenerate primers and genome walking; title: Construction of hairpin RNA-expressing vectors for RNA-mediated gene silencing in fungi; title: An unbiased method for the quantitation of disease phenotypes using a custom-built macro plugin for the program imageJ.