IGF-1 transfection enhances cardiac smooth muscle cell engraftment.
Published: 2004
Language: eng
Number of pages: 103
Description: Results. IGF-I gene transfection increase IGF-1 and vascular endothelial growth factor (VEGF) in cultured smooth muscle cells (SMCs) as compared to plasmid transfected and unmodified SMCs. IGF-1 induced greater cell proliferation and more vascular structures from endothelial cells and decreased caspase-3 activity after ischemia-reperfusion. Transplantation of IGF-1-transfected SMCs into damaged myocardium increased IGF-1 and VEGF levels and vessel density. Fewer apoptotic cells and higher survival rate were in the IGF-1 group than the controls.Congestive heart failure is a major medical problem. Cell transplantation is a promising approach to repair the "broken heart" by replacing scar tissue with muscle cells. However, cell loss after implantation diminishes the functional benefit of cell transplantation.Conclusions. IGF-1 gene improved SMC engraftment in the damaged myocardium, which fulfills the promise of cardiac restoration by cell transplantation.Hypothesis. Insulin-like growth factor 1 (IGF-1) gene could enhance cell survival and improve cell engraftment after transplantation.
Notes: Adviser:  Renke Li.

Thesis (M.Sc.)--University of Toronto, 2004.

Electronic version licensed for access by U. of T. users.

Source: Masters Abstracts International, Volume: 42-06, page: 2157.

MICR copy on microfiche (2 microfiches).