Neisseria meningitidis
Subtitle: advanced methods and protocols
By: edited by Myron Christodoulides
Series: Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 799; Methods in molecular biology (Clifton, N.J.) -- v. 799.; Springer protocols
Publisher: Humana Press; Springer
Published: 2012
Place of publication: New York
Language: eng
Number of pages: 427
ISBN-13: 9781617793455; 9781617793462
Subjects: Neisseria meningitidis; Laboratory Manuals; Bacteriological Techniques
Notes: Includes bibliographical references and index.
Table of contents: title: Neisseria meningitidis: biology, microbiology, and epidemiology; title: Classification and pathogenesis of meningococcal infections; title: Detection of Neisseria meningitidis in cerebrospinal fluid using a multiplex PCR and the luminex detection technology; title: Generating knock-out and complementation strains of Neisseria meningitidis; title: Identification and functional characterization of sRNAs in Neisseria meningitidis; title: Expression, purification, and crystallization of neisserial outer membrane proteins; title: Genome-scale metabolic models: reconstruction and analysis; title: TMT labelling for the quantitative analysis of adaptive responses in the meningococcal proteome; title: Meningococcal ligands and molecular targets of the host; title: In vivo imaging of meningococcal disease dynamics; title: Methods for studying Neisseria meningitidis biofilms; title: Laminar-flow chamber assay for measuring bacterial adhesion under shear stress; title: Techniques to measure pilus retraction forces; title: Human dendritic cell culture and bacterial infection; title: Hydrogen-deuterium exchange coupled to mass spectrometry to investigate ligand-receptor interactions; title: Visualising PAMP-PRR interactions using nanoscale imaging; title: Transcriptome analyses in the interaction of Neisseria meningitidis with mammalian host cells; title: Use of the pan-Neisseria microarray and experimental design for transcriptomics studies of Neisseria; title: Analysis of parameters associated with prevention of cellular apoptosis by pathogenic Neisseriae and purified porins; title: Analysis of the immune response to Nia meningitidis using a proteomics approach; title: Antigen identification starting from the genome:  a "reverse vaccinology" approach applied to MenB; title: DNA vaccine strategy for effective antibody induction to pathogen-derived antigens.