Rna Protein Interaction Protocols
Subtitle: Preliminary Entry 2139 (Methods in Molecular Biology)
Edition: 2 Revised edition
Publisher: Humana Press
Published: November 2008
Language: eng
Format: Hardcover
Number of pages: 400
ISBN-13: 9781588294197
Subjects: Genetics (non-medical); Molecular biology; Science; Science/Mathematics; Life Sciences - Biology - Molecular Biology
First sentence: The problems of isolating sufficient quantities of rare RNAs for detailed biochemical analysis can be circumvented by synthesis of the desired RNA in vitro (1-3).
Description: Due to the vital biological importance of RNA and proteins functioning together within a cell, a protocol volume describing experimental procedures to study their interactions should find a home in many laboratories. This second edition complements and expands upon the popular first edition by providing a collection of cutting-edge techniques developed or refined in the past few years along with tried-and-true methods. The contributors explore the isolation and characterization of RNA-protein complexes, the analysis and measurement of RNA-protein interaction, and related novel techniques and strategies. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.