Yeast genetics
Subtitle: methods and protocols
By: edited by Jeffrey S. Smith, Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA, USA, Daniel J. Burke, Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA, USA,
Series: Methods in molecular biology -- 1205; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v.1205.; Springer protocols (Series)
Publisher: Humana Press
Published: 2014
Place of publication: New York
Language: eng
Number of pages: 378
ISBN-13: 9781493913626; 9781493913633
Subjects: Genetics; Yeast fungi; Yeasts; Laboratory Manuals; Saccharomyces cerevisiae
Work titles: Yeast genetics (Smith)
Description: "Yeast Genetics: Methods and Protocols is a collection of methods to best study and manipulate Saccharomyces cerevisiae, a truly genetic powerhouse. The simple nature of a single cell eukaryotic organism, the relative ease of manipulating its genome, and the ability to interchangeably exist in both haploid and diploid states have always made it an attractive model organism. Genes can be deleted, mutated, engineered, and tagged at will. Saccharomyces cerevisiae, has played a major role in the elucidation of multiple conserved cellular processes including MAP kinase signaling, splicing, transcription, and many others. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Yeast Genetics: Methods and Protocols will provide a balanced blend of classic and more modern genetic methods relevant to a wide range of research areas and should be widely used as a reference in yeast labs"--
Notes: "No copyright is claimed in works prepared by an officer or employee of the United States Government as partof that person's official duties"--Verso of title page.

Includes bibliographical references and index.
Table of contents: title: Yeast transformation by the LiAc/SS carrier DNA/PEG method  -- R. Daniel Gietz; title: Tetrad, random spore, and molecular analysis of meiotic segregation and recombination  -- Michael Lichten; title: PCR mutagenesis and gap repair in yeast  -- Mark Weir and Jill B. Keeney; title: PCR-mediated epitope tagging of genes in yeast  -- Radhika Mathur and Peter Kaiser; title: Manipulating the yeast genome : deletion, mutation, and tagging by PCR  -- Jennifer M. Gardner and Sue L. Jaspersen; title: Preparation of yeast cells for live-cell imaging and indirect immunofluorescence  -- Lucy F. Pemberton; title: Single yeast cell imaging  -- Heimo Wolinski and Sepp D. Kohlwein; title: Microfluidic platforms for generating dynamic environmental perturbations to study the responses of single yeast cells  -- Anjali Bisaria, Pascal Hersen, and Megan N. McClean; title: Using two-hybrid interactions to identify separation-of- function mutations  -- Brian Haarer and David C. Amberg; title: Synthetic genetic array analysis for global mapping of genetic networks in yeast  -- Elena Kuzmin [and six others]; title: Chemical genetic and chemogenomic analysis in yeast  -- Namal V. C. Coorey [and three others]; title: Phenomic assessment of genetic buffering by kinetic analysis of cell arrays  -- John Rodgers, Jingyu Guo, and John L. Hartman IV; title: Detection of short-range chromatin interactions by chromosome conformation capture (3C) in yeast  -- Badri Nath Singh and Michael Hampsey; title: Chromosome conformation capture (3C) of tandem arrays in yeast  -- Maria D. Mayán and Luis Aragón; title: Global analysis of transcription factor-binding sites in yeast using ChIP-Seq  -- Philippe Lefrançois, Jennifer E. G. Gallagher, and Michael Snyder; title: High-density tiling microarray analysis of the full transcriptional activity of yeast  -- Lior David, Sandra Clauder-Münster, and Lars M. Steinmetz; title: Analysis of silencing in Saccharomyces cerevisiae  -- Andrew Miller and Ann L. Kirchmaier; title: A user's guide to the ribosomal DNA in Saccharomyces cerevisiae  -- Joseph M. Johnson, Jeffrey S. Smith, and David A. Schneider; title: Two-dimensional agarose gel electrophoresis for analysis of DNA replication  -- Sandra K. Villwock and Oscar M. Aparicio; title: Replicative life span analysis in budding yeast  -- George L. Sutphin, Joe R. Delaney, and Matt Kaeberlein; title: Metabolomic and lipidomic analyses of chronologically aging yeast -- Vincent R. Richard, Simon D. Bourque, and Vladimir I. Titorenko.