Mycobacteria protocols.
Contributors: Parish, Tanya.; Brown, Amanda Claire.
Edition: 2nd ed. / edited by Tanya Parish and Amanda Claire Brown.
Series: Methods in molecular biology -- v. 465
Publisher: Humana Press
Published: 2008
Place of publication: New York, NY
Language: eng
Number of pages: 456
ISBN-13: 9781588298898
Subjects: Mycobacteria -- Laboratory manuals; Mycobacterium -- Laboratory Manuals; Genetic Techniques -- Laboratory Manuals; Molecular Biology -- methods -- Laboratory Manuals; Cytological Techniques -- Laboratory Manuals
Genres: Laboratory manuals; Laboratory Manuals
Notes: Includes bibliographical references and index.
Table of contents: title: Isolation of Mycobacterium species genomic DNA / John T. Belisle, Spencer B. Mahaffey, and Preston J. Hill; title: Isolation of mycobacterial RNA / Tige R. Rustad ... [et al.]; title: Lipoglycans of Mycobacterium tuberculosis : isolation, purification, and characterization / Libin Shi, Jordi B. Torrelles, and Delphi Chatterjee; title: Analyzing lipid metabolism : activation and [beta]-oxidation of fatty acids / Paul Robert Wheeler; title: Whole genome analysis using microarrays / S.J. Waddell, J. Hinds, and P.D. Butcher; title: Use of DNA microarrays to study global patterns of gene expression / Roberta Provvedi, Giorgio Palù, and Riccardo Manganelli; title: Two-dimensional gel electrophoresis-based proteomics of mycobacteria / Jens Mattow ... [et al.]; title: Transport assays and permeability in pathogenic mycobacteria / Marie-Antoinette Lanéelle and Mamadou Daffé; title: Continuous culture of mycobacteria / Joanna Bacon and Kim A. Hatch; title: Measuring minimum inhibitory concentrations in mycobacteria / Frederick A. Sirgel, Ian J.F. Wiid, and Paul D. van Helden; title: Rapid screening of inhibitors of Mycobacterium tuberculosis growth using tetrazolium salts / Anita G. Amin ... [et al.]; title: Electroporation of mycobacteria / Renan Goude and Tanya Parish; title: Ins and outs of mycobacterial plasmids / Farahnaz Movahedzadeh and Wilbert Bitter; title: The use of temperature-sensitive plasmids in mycobacteria / Damien Portevin, Wladimir Malaga, and Christophe Guilhot; title: Heterologous expression of genes in mycobacteria / James A. Triccas and Anthony A. Ryan; title: Inducible expression systems for mycobacteria / Christopher M. Sassetti; title: Assaying promoter activity using LacZ and GFP as reporters / Paul Carroll and Jade James; title: Construction of unmarked deletion mutants in mycobacteria / Houhui Song, Frank Wolschendorf, and Michael Niederweis; title: Construction of targeted mycobacterial mutants by homologous recombination / Sharon L. Kendall and Rosangela Frita; title: Phage transposon mutagenesis / M. Sloan Siegrist and Eric J. Rubin; title: Gene essentiality testing in Mycobacterium smegmatis using specialized transduction / Apoorva Bhatt and William R. Jacobs, Jr.; title: Gene switching and essentiality testing / Amanda Claire Brown; title: Insertion element IS6110-based restriction fragment length polymorphism genotyping of Mycobacterium tuberculosis / Robin M. Warren, Paul D. van Helden, and Nicolaas C. Gey van Pittius; title: Typing Mycobacterium tuberculosis using variable number tandem repeat analysis / T.J. Brown, V.N. Nikolayevskyy, and F.A. Drobniewski; title: Molecular detection of drug-resistant Mycobacterium tuberculosis with a scanning-frame oligonucleotide microarray / Dmitriy V. Volokhov ... [et al.]; title: MycoDB : an online database for comparative genomics of the mycobacteria and related organisms / Roy R. Chaudhuri; title: Environmental amoebae and mycobacterial pathogenesis / Melanie Harriff and Luiz E. Bermudez.