Mapping the distribution of cell surface molecules by chromophore localization in the transmission electron microscope via low electron energy loss imaging.
Published: 2004
Language: eng
Number of pages: 70
Description: From cell surface studies involving ruthenium red, trypan blue and two lipophilic PKH dyes, the importance of chromophore preservation during specimen preparation was realized. The stability of the binding of fluorescein conjugated cholera toxin to the sphingolipid, GM1 permitted energy filtered imaging and lipid raft localization in T cells with a spatial resolution that was over 100-fold better than light microscopy.We are using a technique developed in our lab to map the distribution of chromophore-tagged cell surface molecules by electron microscopy. The technique employs the use of our previously modified electron energy filter that permits imaging with an energy resolution of 1 eV. Such an energy filter makes it possible to select electrons that in traversing the specimen, have lost energies of only a few electron volts, corresponding to absorption energies of visible colours. Our approach is aimed at imaging cells that have been stained with conventional dyes that are commonly used in light microscopy but delivering such images with the spatial resolution of the electron microscope.
Notes: Adviser:  F. Peter Ottensmeyer.

Thesis (M.Sc.)--University of Toronto, 2004.

Electronic version licensed for access by U. of T. users.

Source: Masters Abstracts International, Volume: 43-03, page: 0796.

MICR copy on microfiche (1 microfiche).