Antiviral
Subtitle: methods and protocols
By: edited by Edwin Yunhao Gong
Edition: 2nd ed.
Series: Methods in Molecular Biology -- 1030; Methods in molecular biology (Clifton, N.J.) -- v. 1030.
Publisher: Humana Press; Springer
Published: 2013
Place of publication: New York
Language: eng
Number of pages: 452
ISBN-13: 9781627034838; 9781627034845
Subjects: Antiviral Agents
Notes: Includes bibliographical references and index.
Table of contents: title: Part I; title: Human immunodeficiency virus type 1 (HIV-1) --; title: Fluorescence-based high-throughput screening assay to identify HIV-1 inhibitors  -- Peggy Geluykens ... [et al.]; title: Homogeneous time-resolved fluorescence assay to identify inhibitors of HIV-1 fusion  -- Liesbet Smeulders ... [et al.]; title: Identification of HIV-1 reverse transcriptase inhibitors using a scintillation proximity assay  -- Bertrand Van Schoubroeck ... [et al.]; title: Biochemical screening assays to identify HIV-1 integrase inhibitors  -- Marleen Clynhens ... [et al.]; title: HIV-1 genotyping of the protease-reverse transcriptase and integrase genes to detect mutations that confer antiretroviral resistance  -- Peter Van den Eede ... [et al.] -- Part II; title: Hepatitis viruses --; title: In vitro kinetic profiling of hepatitis C virus NS3 protease inhibitors by progress curve analysis  -- Rumin Zhang and William T. Windsor; title: Novel hepatitis C virus NS5B polymerase assay of de novo initiated RNA synthesis directed from a heteropolymeric RNA template  -- Eric Ferrari and Hsueh-Cheng Huang; title: Selecting and characterizing drug-resistant hepatitis C virus replicon  -- Inge Vliegen, Leen Delang, and Johan Neyts; title: Cellular replicon-based phenotyping assay to determine susceptibility of hepatitis C virus clinical isolates to NS3/4A protease inhibitors  -- Leen Vijgen ... [et al.]; title: Expression and purification of hepatitis C virus protease from clinical samples  -- Liesbet van der Helm; title: Surface plasmon resonance as a tool to select potent drug candidates for hepatitis C virus NS5B polymerase  -- Erna Cleiren, Benoit Devogelaere, and Katleen Fierens; title: Amplification and sequencing of the hepatitis C virus NS3/4A protease and the NS5B polymerase regions for genotypic resistance detection of clinical isolates of subtypes 1a and 1b  -- Diana Koletzki ... [et al.]; title: Southern blot assay for detection of hepatitis B virus covalently closed circular DNA from cell cultures  -- Dawei Cai ... [et al.]; title: In vitro phenotyping of recombinant hepatitis B virus containing the polymerase/reverse transcriptase gene from clinical isolates  -- Yang Liu and Kathryn M. Kitrinos -- Part III; title: Flaviviruses --; title: Cell-based antiviral assays for screening and profiling inhibitors against dengue virus  -- Edwin Yunhao Gong ... [et al.]; title: Duplex real-time RT-PCR assay for profiling inhibitors of four dengue serotypes  -- Edwin Yunhao Gong ... [et al.]; title: High-throughput screening using dengue virus reporter genomes  -- Wolfgang Fischl and Ralf Bartenschlager; title: Fluorimetric and HPLC-based dengue virus protease assays using a FRET substrate  -- Christoph Nitsche and Christian D. Klein; title: Expression and purification of dengue virus NS5 polymerase and development of a high-throughput enzymatic assay for screening inhibitors of dengue polymerase  -- Edwin Yunhao Gong ... [et al.]; title: Detection and quantification of flavivirus NS5 methyl- transferase activities  -- Siew Pheng Lim, Christophe Bodenreider, and Pei-Yong Shi; title: Testing antiviral compounds in a dengue mouse model  -- Wouter Schul, Andy Yip, and Pei-Yong Shi; title: Construction of self-replicating subgenomic west nile virus replicons for screening antiviral compounds  -- Sofia L. Alcaraz-Estrada, Erin Donohue Reichert,  and Radhakrishnan Padmanabhan -- Part IV; title: Herpes viruses --; title: Methods for screening and profiling inhibitors of herpes simplex viruses  -- Edwin Yunhao Gong; title: In vivo evaluation of antiviral efficacy against genital herpes using mouse and guinea pig models  -- Frances Valencia, Ronald L. Veselenak, and Nigel Bourne; title: Fluorescence-based high-throughput screening assay for identifying human cytomegalovirus inhibitors  -- Christel Van den Eynde ... [et al.] -- Part V; title: Respiratory viruses --; title: Fluorescence-based high-throughput antiviral compound screening assay against respiratory syncytial virus  -- Leen Kwanten, Ben De Clerck, and Dirk Roymans; title: Screening and evaluation of anti-respiratory syncytial virus compounds in cultured cells  -- Anna Lundin, Tomas Bergström, and Edward Trybala; title: Evaluation of antiviral efficacy against human respiratory syncytial virus using cotton rat and mouse models  -- Joke Van den Berg, Leen Kwanten, and Dirk Roymans; title: In vivo evaluation of antiviral compounds on respiratory syncytial virus using a juvenile vervet monkey (Chlorocebus pygerythrus) infection model  -- Lieselot Houspie ... [et al.]; title: Methods to determine mechanism of action of anti-influenza inhibitors  -- Angela Luttick, Stephanie Hamilton, and Simon P. Tucker; title: Methods for evaluation of antiviral efficacy against influenza virus infections in animal models  -- Donald F. Smee and Dale L. Barnard -- Part VI; title: Alphaviruses --; title: Development of a high-throughput antiviral assay for screening inhibitors of chikungunya virus and generation of drug-resistant mutations in cultured cell  -- Edwin Yunhao Gong ... [et al.]; title: Mouse model of chikungunya virus with utility in antiviral studies -- Ashley Dagley and Justin G. Julander.