Hypothalamic control of circadian rhythms in aortic smooth muscle cells.
Published: 2007
Language: eng
Number of pages: 132
ISBN-13: 9780494273937
Description: The hypothalamic master clock coordinates circadian rhythms in peripheral tissues, including the vasculature. Evidence suggests peripheral clocks act to integrate time-of-day information from neural or hormonal signals, regulating gene expression, and subsequently organ physiology. Using the vascular smooth muscle cell line Movas-1, we investigated three potential hormonal signals, and the effect of the circadian clock on vascular gene transcription. Norepinephrine and forskolin treatment synchronized bmal1 mRNA expression. Whereas the potential hormonal signals arginine-vasopressin, vasoactive-intestinal peptide and gastrin-releasing peptide did not alter or synchronize  bmal1 or per1 expression. Serum synchronization of Movas-1 induced cycling of timp1, timp3, col3a1, sm22alpha and cnn1 mRNA. Microarray and real-time RT-PCR analysis of mouse aortic tissue revealed ultradian rhythms in these genes similar to the cycling observed in Movas-1. This work provides insight into the hormonal regulation of the vascular clock, and implicates the circadian machinery as an important regulator of gene expression in this tissue.
Notes: Source: Masters Abstracts International, Volume: 45-06, page: 3028.

Thesis (M.Sc.)--University of Toronto, 2007.

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