System-Wide Studies of Gene Expression in Escherichia coli by Fluorescence Microscopy and High Throughput Sequencing
Contributors: Xie, Xiaoliang S.; Losick, Richard; Murray, Andrew; Rubin, Eric
Published: 2012
Language: eng
Description: Gene expression is a fundamental process in the cell and is made up of two parts - the information flow from DNA to RNA, and from RNA to protein. Here, we examined specific sub-processes in Escherichia coli gene expression using newly available tools that permit genome-wide analysis. We begin our studies measuring mRNA and protein abundances in single cells by single-molecule fluorescence microscopy, and then focus our attention to studying RNA generation and degradation by high throughput sequencing.
Notes: Keywords: bacteria, live-cell imaging, protein noise, quantitative biology, RNA dynamics, single molecule microscopy.

Thesis Ph.D. Harvard University 2012