Single-molecule techniques
Subtitle: a laboratory manual
By: edited by Paul R. Selvin, Taekjip Ha.
Contributors: Selvin, Paul R.; Ha, Taekjip.
Publisher: Cold Spring Harbor Laboratory Press
Published: 2008
Place of publication: Cold Spring Harbor, N.Y
Language: eng
Number of pages: 507
ISBN-13: 9780879697761; 9780879697754
Subjects: Molecular biology -- Laboratory manuals; Biomolecules -- Analysis -- Laboratory manuals; Fluorescence microscopy -- Laboratory manuals; Atomic force microscopy -- Laboratory manuals; Ultrastructure (Biology) -- Laboratory manuals
Genres: Laboratory manuals.
Notes: Includes bibliographical references and index.
Table of contents: title: The new era of biology in singulo / T. Ha and P.R. Selvin; title: Single-molecule FRET with total internal reflection microscopy / C. Joo and T. Ha; title: In vitro and in vivo FIONA and other acronyms for watching molecular motors walk / P.R. Selvin, T. Lougheed, M.T. Hoffman, H. Park, H. Balci, B.H. Blehm, and E. Toprak; title: Colocalization of fluorescent probes: Accurate and precise registration with nanometer resolution / L.S. Churchman and J.A. Spudich; title: Alternating-laser excitation of single molecules / A.N. Kapanidis, M. Heilemann, E. Margeat, X. Kong, E. Nir, and S. Weiss; title: Orientation and rotational motions of single molecules by polarized total internal reflection fluorescence microscopy / J.F. Beausang, Y. Sun, M.E. Quinlan, J.N. Forkey, and Y.E. Goldman; title: Imaging gene expression in living cells at the single-molecule level / J. Xiao, J. Elf, G.-W. Li, J. Yu, and X.S. Xie; title: Single-virus tracking in live cells / M.J. Rust, M. Lakadamyali, B. Brandenburg, and X. Zhuang; title: Ultrasensitive imaging in live cells using fluorescent quantum dots / S. Courty and M. Dahan; title: Imaging real-time gene expression in living systems / A.L. Wells, J.S. Condeelis, R.H. Singer, and D. Zenklusen; title: Single-molecule imaging of stochastic signaling events in living cells / S. Matsuoka, Y. Miyanaga, T. Yanagida, and M. Ueda; title: Fluorescence correlation spectroscopy in vitro and in vivo / E. Haustein and P. Schwille; title: Optical traps to study properties of molecular motors / J.A. Spudich, S.E. Rice, R.S. Rock, T.J. Purcell, and H.M. Warrick; title: High-resolution dual-trap optical tweezers with differential detection / C. Bustamante, Y.R. Chemla, and J.R. Moffitt; title: Imaging and nanomanipulation of an actomyosin motor / S. Nishikawa, T. Komori, T. Ariga, T. Okada, M. Morimatsu, Y. Ishii, and T. Yanagida; title: Single-molecule studies using magnetic traps / T. Lionnet, J.-F. Allemand, A. Revyakin, T.R. Strick, O.A. Saleh, D. Bensimon, and V. Croquette; title: Probing polysaccharide and protein mechanics by atomic force microscopy / M. Rabbi and P.E. Marszalek; title: Single-molecule analysis of nucleic acids and DNA-protein interactions using nanopores / M. Wanunu and A. Meller; title: Single-molecule gold-nanoparticle tracking with high temporal and spatial resolution and without an applied load / A.R. Dunn and J. A. Spudich; title: Advances in surface-based assays for single molecules / P.M. Fordyce, M.T. Valentine, and S.M. Block; title: Hydrodynamic flow-stretching assay for single-molecule studies of nucleic acid-protein interactions / C.M. Schroeder, P.C. Blainey, S. Kim, and X.S. Xie.