Synthetic biology
By: edited by Karen M. Polizzi, Cleo Kontoravdi
Series: Methods in molecular biology -- 1073; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 1073.; Springer protocols (Series)
Publisher: Humana Press; Humana
Published: 2013
Place of publication: New York
Language: eng
Number of pages: 226
ISBN-13: 9781627036245; 9781637036252
Subjects: Synthetic biology; Biotechnology; Synthetic Biology; Methods
Description: In Synthetic Biology , expert researchers in the field provide the latest developments in molecular biology techniques used in Synthetic Biology. Focusing on computational tools that will aid in systematising the design and construction of parts and systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Synthetic Biology seeks to aid scientists in the further study of developing new biological components and systems. --
Notes: Includes bibliographical references and index.
Table of contents: title: What is synthetic biology?  -- Karen M. Polizzi; title: Gene synthesis method based on overlap extension PCR and DNAWorks program  -- Gang Li ... [et al.]; title: BioBrick[TM] assembly using the in-fusion PCR cloning kit  -- Sean C. Sleight and Herbert M. Sauro; title: Overlap extension PCR cloning  -- Anton Bryksin and Ichiro Matsumura; title: One-step isothermal assembly of DNA fragments  -- Rui T.L. Rodrigues and Travis S. Bayer; title: Creation and characterization of component libraries for synthetic biology  -- Tim Weenink and Tom Ellis; title: In vivo and in vitro characterization of [sigma]⁷⁰ constitutive promoters by real-time PCR and fluorescent measurements  -- James Chappell and Paul Freemont; title: In vivo screening of artificial small RNAs for silencing endogenous genes in Escherichia coli  -- Vandana Sharma and Yohei Yokobayashi; title: Construction and engineering of large biochemical pathways via DNA assembler  -- Zengyi Shao and Huimin Zhao; title: Assembly of multi-gene pathways and combinatorial pathway libraries through ePathBrick vectors  -- Peng Xu and Mattheos A.G. Koffas; title: Tandem recombineering by SLIC cloning and Cre-LoxP fusion to generate multigene expression constructs for protein complex research  -- Matthias Haffke ... [et al.]; title: Combinatorial DNA assembly using Golden Gate cloning  -- Carola Engler and Sylvestre Marillonnet; title: Construction of synthetic gene circuits in the Escherichia coli genome  -- Bei-Wen Ying, Yuya Akeno, and Tetsuya Yomo; title: Shuffling of DNA cassettes in a synthetic integron  -- David Bikard and Didier Mazel; title: Systematic methodology for the development of mathematical models for biological processes  -- Cleo Kontoravdi; title: SOBOLHDMR : a general-purpose modeling software -- Sergei Kucherenko.