Gene targeting and embryonic stem cells
By: [edited by] Alison Thomson, Jim McWhir
Contributors: Thomson, Alison; McWhir, Jim
Publisher: Garland Science/BIOS Scientific; Distributed in the USA by Taylor & Francis
Published: 2004
Place of publication: London; New York; Independence, KY
Language: eng
Number of pages: 205
Subjects: Gene targeting; Embryonic stem cells
Other titles: Gene targeting & embryonic stem cells
Description: Embryonic stem (ES) cells are increasingly providing their value, not only in research aimed at understanding gene gunction and human disease, but also as a potential means to treat these diseases. This volume is designed to be a practical guide for the rapidly growing researchers in this field. Details are provided on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for anumber of cell types, and include protocols for gene-targeting vectors, DNA transfection and RNA interference.
Notes: Includes bibliographical references and index