Plant developmental biology
Subtitle: methods and protocols
By: edited by Lars Hennig and Claudia Köhler
Series: Methods in molecular biology -- 655; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 655.; Springer protocols
Publisher: Humana
Published: 2010
Place of publication: New York
Language: eng
Number of pages: 448
ISBN-13: 9781607617648; 9781607617655
Subjects: Plants; Developmental Biology; Methods; Genetics; Plant molecular biology; Growth & development; Research; Plant cell development; Laboratory Manuals
Description: The beauty and variety of plants has fascinated mankind for thousands of years, and this fascination continues today as plant developmental biologists discover mechanisms that control the establishment of specialized cell types, tissues, and organs from the fertilized egg during a plant's life. In Plant Developmental Biology: Methods and Protocols, expert researchers provide a collection of protocols for many of the common experimental approaches in plant developmental biology, including diverse methods that range from grafting over bimolecular fluorescence complementation to chromatin immunoprecipitation. Chapters are divided into six major parts: growth protocols, manipulation of gene activity, assaying developmental phenotypes, assaying gene activity, testing protein-protein interactions, and probing chromatin. Composed in the highly successful Methods in Molecular Biology"!series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and revolutionary, Plant Developmental Biology: Methods and Protocols is an essential guide for all plant developmental geneticists, biochemists, and researchers in stress physiology and plant nutrition
Notes: Includes bibliographical references and index.
Table of contents: title: Growth protocols for model plants in developmental biology / Lars Hennig; title: Grafting as a research tool / Colin G.N. Turnbull; title: Virus-induced gene silencing as a reverse genetics tool to study gene function / Steven Bernacki ... [et al.]; title: The CRE/lox system as a tool for developmental studies at the cell and tissue level / Guy Wachsman and Renze Heidstra; title: Inducible gene expression systems for plants / Lorenzo Borghi; title: Trichome development in Arabidopsis / Joachim F. Uhrig and Martin Hülskamp; title: Phenotyping the development of leaf area in Arabidopsis thaliana / Sarah J. Cookson ... [et al.]; title: Analyzing shoot apical meristem development / Cristel C. Carles ... [et al.]; title: Analyzing floral meristem development / Elisa Fiume ... [et al.]; title: Female gametophytic mutants : diagnosis and characterization / Ronny Völz and Rita Gross-Hardt; title: Pollen tube development / Mark A. Johnson and Benedikt Kost; title: Analysis of root meristem size development / Serena Perilli and Sabrina Sabatini; title: Phenotypic characterization of photomorphogenic responses during plant development / Thomas Kretsch; title: Kinematic analysis of cell division and expansion / Bart Rymen ... [et al.]; title: Flowering time control / Yvonne Möller-Steinbach, Cristina Alexandre, and Lars Hennig; title: mRNA detection by whole mount in situ hybridization (WISH) or sectioned tissue in situ hybridization (SISH) in Arabidopsis / Yvonne Stahl and Rüdiger Simon; title: Immunolocalization of proteins in plants / Michael Sauer and Jiří Friml; title: Detection of small non-coding RNAs / Tamas Dalmay; title: Quantitative real time PCR in plant developmental biology / Vivien Exner; title: Luciferase and green fluorescent protein reporter genes as tools to determine protein abundance and intracellular dynamics / András Viczián and Stefan Kircher; title: Fluorescence-activated cell sorting in plant developmental biology / Anjali S. Iyer-Pascuzzi and Philip N. Benfey; title: Laser microdissection of paraffin-embedded plant tissues for transcript profiling / Robert C. Day; title: Utilizing bimolecular fluorescence complementation (BiFC) to assay protein-protein interaction in plants / Nir Ohad and Shaul Yalovsky; title: The split luciferase complementation assay / Naohiro Kato and Jason Jones; title: Co-immunoprecipitation and protein blots / Erika Isono and Claus Schwechheimer; title: Probing protein-protein interactions with FRET-FLIM / Christoph Bücherl ... [et al.]; title: Plant chromatin immunoprecipitation / Corina B.R. Villar and Claudia Köhler; title: Immunocytological analysis of chromatin in isolated nuclei / Penka Pavlova ... [et al.]; title: Bisulphite sequencing of plant genomic DNA / Ernst Aichinger and Claudia Köhler.