Single molecule enzymology
Subtitle: methods and protocols
By: edited by Gregory I. Mashanov, Christopher Batters
Series: Methods in molecular biology -- v. 778; Springer protocols
Publisher: Humana Press; Springer
Published: 2011
Place of publication: New York
Language: eng
Number of pages: 294
ISBN-13: 9781617792601; 9781617792618
Subjects: Enzymes; Laboratory Manuals; Chemistry; Kinetics; Metabolism
Notes: Includes bibliographical references and index.
Table of contents: title: Single enzyme studies : a historical perspective  -- Alex E. Knight; title: Functional surface attachment in a sandwich geometry of GFP-labeled motor proteins  -- Volker Bormuth ... [et al.]; title: Studying kinesin's enzymatic cycle using a single-motor confocal motility assay, employing Förster resonance energy transfer  -- Zdenek Lansky and Erwin J.G. Peterman; title: Fluorescence imaging with one nanometer accuracy : in vitro and in vivo studies of molecular motors  -- Melinda Tonks Hoffman, Janet Sheung, and Paul R. Selvin; title: Snapshots of kinesin motors on microtubule tracks  -- Franck J. Fourniol and Carolyn A. Moores; title: Structural and dynamic characterization of biochemical processes by atomic force microscopy -- Frédéric Eghiaian and Iwan A. T. Schaap; title: Using optical tweezers to study the fine details of myosin ATPase mechanochemical cycle  -- Christopher Batters and Claudia Veigel; title: Quantum dot labeling strategies to characterize single-molecular motors ; title: ^ -- Shane R. Nelson, M. Yusuf Ali, and David M. Warshaw; title: Imaging individual myosin molecules within living cells  -- Tatiana A. Nenasheva ... [et al.]; title: Single-molecule measurements using microneedles  -- Toshio Yanagida, Yoshiharu Ishii, and Akihiko Ishijima; title: Fluorescent nucleoside triphosphates for single-molecule enzymology  -- Christopher P. Toseland and Martin R. Webb; title: Probing the mechanics of the complete DNA transcription cycle in real-time using optical tweezers  -- Christoph G. Baumann and Stephen J. Cross; title: Single-molecule approach to visualize the unwinding activity of DNA helicases  -- Natalia Fili ... [et al.]; title: Real-time single-molecule observation of green fluorescent protein synthesis by immobilized ribosomes  -- Ryo Iizuka, Takashi Funatsu, and Sotaro Uemura; title: Single-molecule measurements of topoisomerase activity with magnetic tweezers -- Yeonee Seol and Keir C. Neuman; title: ^; title: Assembly of recombinant nucleosomes on nanofabricated DNA curtains for single-molecule imaging  -- Ja Yil Lee and Eric C. Greene; title: Simultaneous observation of chemomechanical coupling of a molecular motor  -- Takayuki Nishizaka, Yuh Hasimoto, and Tomoko Masaike; title: Microsecond resolution of single-molecule rotation catalyzed by molecular motors -- Tassilo Hornung ... [et al.].