Cardiovascular development
Subtitle: methods and protocols
By: edited by Xu Peng, Marc Antonyak
Series: Methods in molecular biology -- 843; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 843.; Springer protocols
Publisher: Humana Press/Springer
Published: 2012
Place of publication: New York
Language: eng
Number of pages: 341
ISBN-13: 9781617795220; 9781617795237
Subjects: Heart; Embryology; Laboratory Manuals; Research; Cardiovascular System; Growth and development; Laboratory manuals; Cardiovascular system
Description: Congenital heart disease is the leading cause of infant death and affects approximately one in every 100 babies born in the United States. The study of cardiovascular development has acquired new momentum in last twenty years due to the advancement of modern molecular biology and new available equipments and techniques. In Cardiovascular Development: Methods and Protocols expert researchers in the field in the field detail many of the methods which are now commonly used in the field of cardiovascular development research. These include methods and techniques for using different organisms for cardiovascular developmental research, using cell and molecular biology methods to study cardiovascular development, as well as other available techniques for cardiovascular development research. Written in the highly successful Methods in Molecular Biology"!series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cardiovascular Development: Methods and Protocols seeks to aid scientists in understanding new state-of-the-art techniques in the field of cardiovascular development research including in vivo imaging and Bioinformatics
Notes: Includes bibliographical reference and index.
Table of contents: title: Use of whole embryo culture for studying heart development / Calvin T. Hang and Ching-Pin Chang; title: Quantifying cardiac functions in embryonic and adult zebrafish / Tiffany Hoage, Yonghe Ding, and Xiaolei Xu; title: Analysis of the patterning of cardiac outflow tract and great arteries with angiography and vascular casting / Ching-Pin Chang; title: Morpholino injection in Xenopus / Panna Tandon ... [et al.]; title: Chicken chorioallantoic membrane angiogenesis model / Domenico Ribatti; title: Visualizing vascular networks in zebrafish : an introduction to microangiography / Christopher E. Schmitt, Melinda B. Holland, and Suk-Won Jin; title: Whole-mount confocal microscopy for vascular branching morphogenesis / Yoh-suke Mukouyama ... [et al.]; title: Visualization of mouse embryo angiogenesis by fluorescence-based staining / Yang Liu, Marc Antonyak, and Xu Peng; title: Miniaturized assays of angiogenesis in vitro / May J. Reed and Robert B. Vernon; title: Analysis of the endocardial-to-mesenchymal transformation of heart valve development by collagen gel culture assay / Yiqin Xiong, Bin Zhou, and Ching-Pin Chang; title: Quantification of myocyte chemotaxis : a role for FAK in regulating directional motility / Britni Zajac ... [et al.]; title: Analysis of neural crest cell fate during cardiovascular development using Cre-activated lacZ/[beta]-galactosidase staining / Yanping Zhang and L. Bruno Ruest; title: Indirect immunostaining on mouse embryonic heart for the detection of proliferated cardiomyocyte / Jieli Li, Marc Antonyak, and Xu Peng; title: Isolation and characterization of vascular endothelial cells from murine heart and lung / Yixin Jin ... [et al.]; title: Isolation and characterization of embryonic and adult epicardium and epicardium-derived cells / Bin Zhou and William T. Pu; title: Vascular smooth muscle cells : isolation, culture, and characterization / Richard P. Metz, Jan L. Patterson, and Emily Wilson; title: C-kit expression identifies cardiac precursor cells in neonatal mice / Michael Craven, Michael I. Kotlikoff, and Alyson S. Nadworny; title: Cardiomyocyte apoptosis in heart development : methods and protocols / Dongfei Qi and Mingui Fu; title: Adenovirus-mediated gene transfection in the isolated lymphatic vessels / Anatoliy A. Gashev ... [et al.]; title: Isolation of cardiac myocytes and fibroblasts from neonatal rat pups / Honey B. Golden ... [et al.]; title: The application of genome-wide RNAi screens in exploring varieties of signaling transduction pathways / Shenyuan Zhang and Hongying Zheng; title: Application of atomic force microscopy measurements on cardiovascular cells / Xin Wu ... [et al.]; title: In utero assessment of cardiovascular function in the embryonic mouse heart using high-resolution ultrasound biomicroscopy / Honey B. Golden ... [et al.]; title: Isolation and preparation of RNA from rat blood and lymphatic microvessels for use in microarray analysis / Eric A. Bridenbaugh; title: Visual data mining of coexpression data to set research priorities in cardiac development research / Vincent VanBuren; title: High-speed confocal imaging of zebrafish heart development / Jay R. Hove and Michael P. Craig; title: Measurement of electrical conduction properties of intact embryonic murine hearts by extracellular microelectrode arrays / David G. Taylor and Anupama Natarajan.