Engineered zinc finger proteins
Subtitle: methods and protocols
By: edited by Joel P. Mackay, David J. Segal
Series: Methods in molecular biology -- v. 649; Springer Protocols; Methods in molecular biology (Clifton, N.J.) -- v.649.; Springer protocols
Publisher: Humana Press
Published: 2010
Place of publication: New York
Language: eng
Number of pages: 500
ISBN-13: 9781607617525; 9781607617532
Subjects: Zinc-finger proteins; Zinc Fingers; Laboratory Manuals; Transcription Factors
Description: Among the many types of DNA binding domains, C2H2 zinc finger proteins (ZFPs) have proven to be the most malleable for creating custom DNA-binding proteins. In Engineered Zinc Finger Proteins: Methods and Protocols, expert researchers from some of the most active laboratories in this field present detailed methods, guidance, and perspectives. The volume contains sections covering the engineering of ZFPs, methods for the creation, evaluation, and delivery of artificial transcription factors (ATFs), methods for the creation and evaluation of zinc finger nucleases (ZFNs), and a collection of the several applications and assays beyond ATFs and ZFNs, including zinc finger transposases and ChIP-seq methodology amongst other subjects. Written in the highly successful Methods in Molecular Biology' series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Engineered Zinc Finger Proteins: Methods and Protocols aims to aid both seasoned practitioners and new investigators with its vital methods and insights as they seek to create the next generation of engineered ZFPs and applications. --Book Jacket.
Notes: Includes bibliographical references and index.

Also available online.
Table of contents: title: The generation of zinc finger proteins by modular assembly / Mital S. Bhakta and David J. Segal; title: Engineering single Cys2His2 zinc finger domains using a bacterial cell-based two-hybrid selection system / Stacey Thibodeau-Beganny, Morgan L. Maeder, and J. Keith Joung; title: Bipartite selection of zinc fingers by phage display for any 9-bp DNA target site / Jia-Ching Shieh; title: Structure-based DNA-binding prediction and design / Andreu Alibés, Luis Serrano, and Alejandro D. Nadra; title: Generation of cell-permeable artificial zinc finger protein variants / Takashi Sera; title: Inhibition of viral transcription using designed zinc finger proteins / Kimberley A. Hoeksema and D. Lorne J. Tyrrell; title: Modulation of gene expression using zinc finger-based artificial transcription factors / Sabine Stolzenburg ... [et al.]; title: Construction of combinatorial libraries that encode zinc finger-based transcription factors / Seokjoong Kim, Eun Ji Kim, and Jin-Soo Kim; title: Silencing of gene expression by targeted DNA methylation : concepts and approaches / Renata Z.  Jurkowska and Albert Jeltsch; title: Remodeling genomes with artificial transcription factors (ATFs) / Adriana S. Beltran and Pilar Blancafort; title: Transgenic mice expressing an artificial zinc finger regulator targeting an endogenous gene / Claudio Passananti ... [et al.]; title: Artificial zinc finger nucleases for DNA cloning / Vardit Zeevi, Andriy Tovkach, and Tzvi Tzfira; title: In vitro assessment of zinc finger nuclease activity / Toni Cathomen and Cem Șöllü; title: Quantification of zinc finger nuclease-associated toxicity / Tatjana I. Cornu and Toni Cathomen; title: A rapid and general assay for monitoring endogenous gene modification / Dmitry Y. Guschin ... [et al.]; title: Engineered zinc finger proteins for manipulation of the human mitochondrial genome / Michal Minczuk; title: High-efficiency gene targeting in Drosophila with zinc finger nucleases / Dana Carroll, Kelly J. Beumer, and Jonathan K. Trautman; title: Using zinc finger nucleases for efficient and heritable gene disruption in zebrafish / Jasmine M. McCammon and Sharon L. Amacher; title: A transient assay for monitoring zinc finger nuclease activity at endogenous plant gene targets / Justin P. Hoshaw ... [et al.]; title: Validation and expression of zinc finger nucleases in plant cells / Andriy Tovkach, Vardit Zeevi, and Tzvi Tzfira; title: Non-FokI-based zinc finger nucleases / Miki Imanishi, Shigeru Negi, and Yukio Sugiura; title: Designing and testing chimeric zinc finger transposases / Matthew H. Wilson and Alfred L. George; title: Seeing genetic and epigenetic information without DNA denaturation using sequence-enabled reassembly (SEER) / Jason R. Porter ... [et al.]; title: Zinc finger-mediated live cell imaging in Arabidopsis roots / Beatrice I. Lindhout, Tobias Meckel, and Bert J. van der Zaal; title: Biophysical analysis of the interaction of toxic metal ions and oxidants with the zinc finger domain of XPA / Andrea Hartwig, Tanja Schwerdtle, and Wojciech Bal; title: Preparation and zinc-binding properties of multi-fingered zinc-sensing domains / John H. Laity and Linda S. Feng; title: Using ChIP-seq technology to identify targets of zinc finger transcription factors / Henriette O'Geen, Seth Frietze, and Peggy J. Farnham; title: Crystallization of zinc finger proteins bound to DNA / Nancy C. Horton and Chad K. Park; title: Beyond DNA : zinc finger domains as RNA-binding modules / Josep Font and Joel P. Mackay.