Distribution of bacteria in a PCE-contaminated pilot aquifer during bioremediation.
Published: 2006
Language: eng
Number of pages: 126
ISBN-13: 9780494211663
Description: This research characterized bacterial distribution in a side-by-side pilot aquifer comparison of natural attenuation (NA), biostimulation with lactate and methanol (ST), and bioaugmentation with a dechlorinating culture (AU) for the remediation of tetrachloroethene-contaminated groundwater. Quantitative PCR methods were used to evaluate the fate of a dechlorinating species,  Dehalococcoides, more than two years post-inoculation, once a starvation period finished through tetrachloroethene-sources replenishment. Greater and more widespread growth of Dehalococcoides in AU than ST emphasized its persistence and quick recovery through fluctuating environmental conditions.  Geobacter, another dechlorinating species, presented a more limited distribution in ST than AU. Neither species were present in NA. PCR-DGGE patterns were used to evaluate the impact of the treatments on the planktonic/sessile microbial communities. Biostimulation affected the planktonic community composition nearest the feeding site, whereas bioaugmentation affected both sessile and planktonic community compositions nearest the injection/feeding site. Finally, NA did not affect either microbial community significantly.
Notes: Source: Masters Abstracts International, Volume: 45-03, page: 1380.

Thesis (M.Sc.)--University of Toronto, 2006.

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