effects of surface concentrations on fibroblast response to collagen-polyacrylic acid modified surfaces.
Published: 2004
Language: eng
Number of pages: 163
Description: Bioactive surfaces are designed to elicit more favourable host responses through specific, targeted, biomolecular interactions between the implant surface and host. Surfaces with type I collagen covalently immobilized to a graft polymerized layer of polyacrylic acid (PAA) were previously shown to promote intimate implant-connective tissue integration. In this thesis, it was hypothesized that PAA graft and immobilized collagen surface concentrations significantly affect in vitro fibroblast response to the above collagen-PAA modified surfaces.Collagen and PAA surface concentrations were found to significantly affect keratocyte and 3T3 cell proliferation, morphology, F-actin cytoskeletal structure and synthesized type I collagen deposition. Concentration dependencies were also functions of the cell response and cell line considered. Two groups of surfaces were of particular interest. 3T3 cell and keratocyte proliferation were significantly lower (p &lt; 0.02) on surfaces with high PAA (&ge;230 mug/cm 2) and high collagen (&ge;4 mug/cm2) concentrations, and comparisons were made with two other collagen matrix systems. Together these systems may provide important insights into cell-matrix signalling pathways and the mechanisms responsible for cell proliferation and quiescence. Of the surfaces examined, those with low PAA (190 mug/cm2) and intermediate collagen (2 to 4 mug/cm2) concentrations appeared best able to promote implant-tissue integration, balancing optimal concentrations ranges for the different cell responses evaluated and allowing for immobilized collagen degradation. Overall, this work demonstrated the importance and potential benefits of optimizing bioactive surface concentrations for each application.
Notes: Adviser:  Yu-Ling Cheng.

Thesis (Ph.D.)--University of Toronto, 2004.

Electronic version licensed for access by U. of T. users.

Source: Dissertation Abstracts International, Volume: 65-05, Section: B, page: 2505.