Investigation of force, kinetochores, and tension in the Saccharomyces cerevisiae mitotic spindle
Contributors: Murray, Andrew W.; Amon, Angelika; Schier, Alex; Denic, Vlad; Losick, Rich; O'Shea, Erin; Michael, Matt
Published: 2013
Language: eng
Description: Cells must faithfully segregate their chromosomes at division; errors in this process causes cells to inherit an incorrect number of chromosomes, a hallmark of birth defects and cancer. The machinery required to segregate chromosomes is called the spindle, a bipolar array of microtubules that attach to chromosomes through the kinetochore. Replicated chromosomes contain two sister chromatids whose kinetochores must attach to microtubules from opposite poles to ensure correct inheritance of chromosomes. The spindle checkpoint monitors the attachment to the spindle and prevents cell division until all chromatids are attached to opposite poles. Both the spindle and the checkpoint are critical for correct segregation, and we sought to understand the regulation of these two components.
Notes: Keywords: Chromosomes, Chromosome segregation, Kinetochores, Mitotic spindle, Spindle checkpoint.

Thesis Ph.D. Harvard University 2013