Protein chromatography
Subtitle: methods and protocols
By: edited by Dermot Walls and Sinéad T. Loughran
Series: Springer protocols; Methods in molecular biology -- v. 681
Publisher: Humana Press
Published: 2011
Place of publication: New York, N.Y
Language: eng
Number of pages: 527
ISBN-13: 9781607619123
Subjects: Methods; Chromatography; Proteomics; Proteins; Analysis; Chromatographic analysis
Notes: Includes bibliographical references and index.
Table of contents: title: A digest of protein purification; title: Gel-filtration chromatography; title: Immunoaffinity chromatography; title: Avoiding proteolysis during protein chromatography; title: Scale-up of protein purification : Downstream processing issues; title: Phage display : A powerful technology for the generation of high specificity affinity reagents from alternative immune sources; title: Engineering protein stability; title: Microfluidics in protein chromatography; title: Tagging recombinant proteins to enhance solubility and aid purification; title: Storage and lyophilisation of pure proteins; title: Differential precipitation and solubilization of proteins; title: Ion-exchange chromatography : Basic principles and application to the partial purification of soluble mammalian prolyl oligopeptidase; title: Protein quantitation and analysis of purity; title: Purification of proteins fused to glutathione S-transferase; title: Purification of proteins fused to maltose-binding protein; title: Purification of proteins from baculovirus-infected insect cells; title: ^; title: Purification of poly-histidine-tagged proteins; title: Immobilized metal affinity chromatography/reversed-phase enrichment of phosphopeptides and analysis by cid/etd tandem mass spectrometry; title: Tag removal by site-specific cleavage of recombinant fusion proteins; title: Purification of antibodies using affinity chromatography; title: Optimized generation of high-affinity, high-specificity single-chain Fv antibodies from multiantigen immunized chickens; title: Measuring protein-protein interactions using biacore; title: Ultra-performance liquid chromatography-mass spectrometry of proteins; title: Hydrophobic interaction chromatography; title: Fast protein liquid chromatography; title: Shotgun proteomics : A qualitative approach applying isoelectric focusing on immobilized pH gradient and LC-MS/MS; title: Shotgun proteomics : A relative quantitative approach using off-gel electrophoresis and LC-MS/MS; title: Clinical proteomics : Liquid chromatography-mass spectrometry purification systems; title: ^; title: ^^; title: Strategies for the purification of membrane proteins; title: A multi-step chromatographic strategy to purify three fungal endo-[beta]-glucanases.; title: ^^