Fluorescence In Situ Hybridization (FISH) — Application Guide
By: edited by Thomas Liehr
Contributors: SpringerLink (Online service)
Publisher: Springer Berlin Heidelberg
Published: 2009
Place of publication: Berlin, Heidelberg
Language: eng
Format: [electronic resource] /
ISBN-13: 9783540705819; 9783540705802
Subjects: Life sciences; Developmental biology; Human genetics; Medicine; Cytology; Reproductive Medicine; Research_xMethodology
Other titles: Springer protocols.
Description: This FISH application guide provides an overview of the principles and the basic techniques of fluorescence in situ hybridization (FISH) and primed in situ hybridization (PRINS), which are successfully used to study many aspects of genomic behavior and alterations. In 36 chapters, contributed by international experts in their particular field, the nowadays multiple approaches and applications of the powerful techniques are presented and detailed protocols are given. Described here are methods using various cell types and tissues as well as different organisms, such as mammalians, insects, plants and microorganisms. Multicolor FISH procedures and special applications such as the characterization marker chromosomes, breakpoints, cryptic aberrations, nuclear architecture and epigenetic changes, as well as (array-based) comparative genomic hybridization studies are presented. Overall, the technique of choice is introduced for single cell analysis in human genetics, microbiology, animal and plant sciences.