Multiple myeloma
Subtitle: methods and protocols
By: edited by Ross D. Brown, P. Joy Ho.
Contributors: Brown, Ross D.; Ho, P. Joy.
Series: Methods in molecular medicine -- 113
Publisher: Humana Press
Published: 2005
Place of publication: Totowa, N.J
Language: eng
Number of pages: 306
Subjects: Multiple myeloma -- Molecular aspects -- Laboratory manuals; Multiple Myeloma -- immunology -- Laboratory Manuals; Multiple Myeloma -- genetics -- Laboratory Manuals
Genres: Laboratory manuals.; Laboratory Manuals.
Description: A comprehensive collection of classic and innovative methodologies used in many laboratories for the investigation of multiple myeloma. These readily reproducible techniques range from the standard Plasma Cell Labeling Index methodology to a final chapter on making sense of microarrays, and include the full spectrum of cytogenetic and molecular diagnostic methods. These proven techniques are ideal for studying the pathogenesis of multiple myeloma and identifying new therapeutic targets.
Notes: Includes bibliographical references and index.
Table of contents: title: Multiple myeloma : challenges and opportunities / Douglas Joshua, Ross Brown, and P. Joy Ho; title: Immunophenotyping of plasma cells in multiple myeloma / Gema Mateo Manzanera, Jesus F. San Miguel Izquierdo, and Alberto Orfao de Matos; title: Plasma cell labeling index / Philip R. Griepp and Shaji Kumar; title: Conventional cytogenetics in myeloma / Lynda J. Campbell; title: Multicolour spectral karyotyping in multiple myeloma / Jeffrey R. Sawyer; title: Detection of chromosome 13 deletions by fluorescent in situ hybridization / Scott van Wier and Rafael Fonseca; title: Comparative genomic hybridization for analysis of changes in DNA copy number changes in multiple myeloma / Pulivarthi H. Rao; title: Southern blotting of IgH rearrangements in B cell disorders / Edna Yuen and Ross D. Brown; title: Identification and assembly of V gene  as idiotype-specific DNA fusion vaccines in multiple myeloma / Surinder S. Sahota, Mark Townsend, and Freda K. Stevenson; title: Identification of clonotypic IgH VDJ sequences in multiple myeloma / Brian J. Taylor ... [et al.]; title: Real-time Polymerase chain reaction of immunoglobulin rearrangements for quantitative evaluation of minimal residual disease in myeloma / Mara Compagno ... [et al.]; title: Incomplete DJH rearrangements / David Gonzalez and Ramon Garcia-Sanz ...; title: Identification of malignant plasma cells by mRNA in situ hybridization / Ross D. Brown and P. Joy Ho; title: The SCID-hu myeloma model / Joshua Epstein and Shmuel Yaccoby; title: The 5T2MM murine model of multiple myeloma : maintenance and analysis / Karin Vanderkerken ... [et al.]; title: Determination of telomerase activity and telomere length / Kai-da Wu and Malcolm A.S. Moore; title: Dendritic cell/myeloma hybrid vaccine / Dajing Xia, Tim Chan, and Jim Xiang; title: Genetically engineered myeloma cell vaccine / Siguo Hao, Tim Chan, and Jim Xiang; title: An in vitro osteoclast-forming assay to measure myeloma cell-derived osteoclast-activating factors / Andrew C.W. Zannettino ... [et al.]; title: Clonality detection of the expanded T-cell populations in patients with multiple myeloma / Daniel M.-Y. Sze; title: Detecting mismatch repair defects in myeloma / Mark Velangi ... [et al.]; title: Methylation-specific polymerase chain reaction / Oliver Galm and James G. Herman; title: Making sense of DNA microarray data / Wei-min Liu, Sunhee K. Ro, and Walter H. Koch.