Homing endonucleases
Subtitle: methods and protocols
By: edited by David R. Edgell, Department of Biochemistry, Schulich School of Medicine and Dentistry, Western University, London, ON, Canada
Series: Methods in molecular biology -- 1123; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 1123.; Springer protocols (Series)
Publisher: Humana Press
Published: 2014
Place of publication: New York
Language: eng
Number of pages: 284
ISBN-13: 9781627039673; 9781627039680
Copyright date: 2014
Subjects: Endonucleases; Laboratory Manuals; Endonucleasen; Labortechnik
Notes: Includes bibliographical references and index.
Table of contents: title: Homing endonucleases : from genetic anomalies to programmable genomic clippers  -- Marlene Belfort and Richard P. Bonocora; title: Bioinformatic identification of homing endonucleases and their target sites  -- Eyal Privman; title: PCR-based bioprospecting for homing endonucleases in fungal mitochondrial rRNA genes  -- Mohamed Hafez [and four others.]; title: Mapping homing endonuclease cleavage sites using in vitro generated protein  -- Richard P. Bonocora and Marlene Belfort; title: Mapping free-standing homing endonuclease promoters using 5'RLM-RACE  -- Ewan A. Gibb; title: PCR analysis of chloroplast double-strand break (DSB) repair products induced by I-CreII in chlamydomonas and arabidopsis  -- Taegun Kwon [and three others ]; title: A two-plasmid bacterial selection system for characterization and engineering of homing endonucleases  -- Ning Sun and Huimin Zhao; title: Rapid screening of endonuclease target site preference using a modified bacterial two-plasmid selection  -- Jason M. Wolfs, Benjamin P. Kleinstiver, and David R. Edgell; title: A yeast-based recombination assay for homing endonuclease activity  -- Jean-Charles Epinat; title: Rapid determination of homing endonuclease DNA binding specificity profile  -- Lei Zhao and Barry L. Stoddard; title: Quantifying the information content of homing endonuclease target sites by single base pair profiling  -- Joshua I. Friedman, Hui Li, and Raymond J. Monnat, Jr.; title: Homing endonuclease target site specificity defined by sequential enrichment and next-generation sequencing of highly complex target site libraries -- Hui Li and Raymond J. Monnat Jr.; title: Homing endonuclease target determination Using SELEX adapted for yeast surface display  -- Kyle Jacoby and Andrew M. Scharenberg; title: Engineering and flow-cytometric analysis of chimeric LAGLIDADG homing endonucleases from homologous I-OnuI-family enzymes  -- Sarah K. Baxter, Andrew M. Scharenberg, and Abigail R. Lambert; title: Bioinformatics identification of coevolving residues  -- Russell J. Dickson and Gregory B. Gloor; title: Identification and analysis of genomic homing  endonuclease target sites  -- Stefan Pellenz and Raymond J. Monnat Jr.; title: Redesigning the specificity of protein-DNA interactions with Rosetta -- Summer Thyme and David Baker.