Nuclear reprogramming
Subtitle: methods and protocols
By: edited by Steve Pells.
Contributors: Pells, Steve.
Series: Methods in molecular biology ;; 325; Methods in molecular biology (Clifton, N.J.) ;; v. 325.
Publisher: Humana Press
Published: 2006
Place of publication: Totowa, N.J
Language: eng
Number of pages: 333
Subjects: Cell nuclei -- Transplantation -- Laboratory methods; Cell Nucleus -- physiology; Cell Fusion -- methods; Transplantation -- methods
Genres: Laboratory methods.
Notes: Includes bibliographical references and index.
Table of contents: title: Nuclear transplantation in xenopus / J. B. Gurdon; title: Nuclear transfer in the sheep / William A. Ritchie; title: Protocols for nuclear transfer in mice / Shaorong Gao; title: Isolation of stromal stem cells from human adipose tissue / Andrew C. Boquest ... [et al.]; title: Nuclear reprogramming by cell fusion / Robert H. Broyles ... [et al.]; title: Polyethylene glycol-mediated cell fusion / Jian Yang and Ming Hong Shen; title: Epigenetic reprogramming of somatic genomes by electrofusion with embryonic stem cells / Masako Tada and Takashi Tada; title: Quantification of cell fusion by flow cytometry / Stephen Sullivan ... [et al.]; title: Modulation of cell fate using nuclear and cytoplasmic extracts / Anne-Mari Høkelien, Kristine G. Gaustad, and Philippe Collas; title: Transgenic systems in nuclear reprogramming / Megan Munsie, Peter Mountford, and Jennifer Nichols; title: Using immunofluorescence to observe methylation changes in mammalian preimplantation embryos / Føtima Santos and Wendy Dean; title: Observing nuclear structure/chromatin changes with fluorescently labeled antibodies / Rong Wu, Anna V. Terry, and David M. Gilbert; title: Quantitative analysis of telomerase activity and telomere length in domestic animal clones / Dean H. Betts ... [et al.]; title: Pluripotency: capacity for in vitro differentiation of undifferentiated embryonic stem cells / Cornelia Wiese ... [et al.]; title: Staining embryonic stem cells using monoclonal antibodies to stage-specific embryonic antigens / Bruce A. Fenderson ... [et al.]; title: Analysis of the nucleolar compartment of the nucleus as an indicator of nuclear reprogramming after nuclear transfer / Jacques-E. Fløchon; title: Methylation-sensitive polymerase chain reaction / Hannah R. Moore, Richard R. Meehan, and Lorraine E. Young; title: Analysis of DNA methylation profiles in preimplantation embryos using bisulfite mutagenesis / Yong-Mahn Han, Seok-Ho Kim, and Yong-Kook Kang; title: Chromatin immunoprecipitation assay for mammalian tissues / Fiona B. Turner, Wang L. Cheung, and Peter Cheung; title: Histone modifications and transcription factor binding on chromatin: CHIP/PCR assays / Jaejoon Won and Tae Kook Kim; title: In vivo genomic footprinting using LM/PCR methods / Hiromi Tagoh, Peter N. Cockerill, and Constanze Bonifer; title: Analyzing histone modification using crosslinked chromatin treated with micrococcal nuclease / Pascal Lefevre and Constanze Bonifer.