PCR/RT-PCR in situ light and electron microscopy
By: Gérard Morel, Mireille Raccurt
Contributors: Raccurt, Mireille
Series: Methods of visualization
Publisher: CRC Press
Published: 2003
Place of publication: Boca Raton, Fla
Language: eng
Number of pages: 416
Subjects: Microscopy -- Technique; Electron microscopy -- Technique; Polymerase chain reaction; Reverse transcriptase
Description: "PCR/RT-PCR in situ Light and Electron Microscopy covers methods of in situ polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR), two new approaches in visualizing very low amounts of DNA and RNA in tissues and cell cultures at the light and electron microscopic levels. Written by experts in this field, the book provides theoretical consideration, as well as practical approaches to in situ PCR.

The authors provide detailed protocols for each step, including the preparation of tissue samples, the rationale for the design of primers, and revelation. They also emphasize the need for appropriate controls to meet the requirements of in situ PCR and RT-PCR specificity. Organized in a user-friendly two-column format, this book will provide you with tools necessary to perform and optimize these sensitive and powerful techniques in your research protocols."--BOOK JACKET.