miRNA maturation
Subtitle: methods and protocols
By: edited by Christoph Arenz, Institute for Chemistry, Humboldt-Universität zu Berlin, Berlin, Germany
Series: Methods in molecular biology -- 1095; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 1095.; Springer protocols (Series)
Publisher: Humana Press; Springer
Published: 2014
Place of publication: New York
Language: eng
Number of pages: 192
ISBN-13: 9781627037020; 9781627037037
Copyright date: 2014
Subjects: RNA; Laboratory manuals; MicroRNAs; Laboratory Manuals; Metabolism
Other titles: Micro RNA maturation
Description: In miRNA Maturation: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study miRNA maturation. These included established methods such as fluorescent and non-fluorescent methods for homogenous assays of Dicer-mediated miRNA maturation or an in vivo assay for Drosha activity. Moreover, the volume also contains useful, but less-common methods that are hard to find elsewhere. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, miRNA Maturation: Methods and Protocols seeks to widen the view on miRNA as biological mediator and potential drug target.
Notes: Includes bibliographical references and index.
Table of contents: title: Pathway of miRNA maturation  -- Michael Sand; title: MicroRNA maturation and human disease  -- Marlen Hesse and Christoph Arenz; title: Approaches to the modulation of miRNA maturation  -- Valerie T. Tripp, Jaclyn R. McKenna, and Douglas D. Young; title: Expression profiling of components of the miRNA maturation machinery  -- Michael Sand and Marina Skrygan; title: Primary MicroRNA processing assay reconstituted using recombinant Drosha and DGCR8  -- Ian Barr and Feng Guo; title: In vivo processing assay based on a dual-luciferase reporter system to evaluate DROSHA enzymatic activity  -- Vera Bilan ... [et al.]; title: Assaying dicer-mediated miRNA maturation by means of fluorescent Substrates  -- Marlen Hesse, Brian P. Davies, and Christoph Arenz; title: Fluorescence correlation spectroscopy-based enzyme assay for human dicer  -- Eileen Magbanua and Ulrich Hahn; title: Detection of microRNA maturation using unmodified pre-microRNA and branched rolling circle amplification  -- Saskia Neubacher and Christoph Arenz; title: Quantitative RT-PCR specific for precursor and mature miRNAs  -- Hannah Zöllner, Stephan A. Hahn, and Abdelouahid Maghnouj; title: Cellular microRNA sensors based on luciferase reporters  -- Colleen M. Connelly and Alexander Deiters; title: Identification of inhibitors of microRNA function from small molecule screens  -- Colleen M. Connelly and Alexander Deiters; title: Inhibition of miRNA maturation by peptide nucleic acids  -- Concetta Avitabile ... [et al.]; title: Molecular methods for validation of the biological activity of peptide nucleic acids targeting microRNAs  -- Eleonora Brognara ... [et al.]; title: Lentiviral overexpression of miRNAs -- Hannah Zöllner, Stephan A. Hahn, and Abdelouahid Maghnouj.