Methods in Enzymology, Volume 312
Subtitle: Sphingolipid Metabolism and Cell Signaling, Part B (Methods in Enzymology)
Contributors: John N. Abelson (Editor); Melvin I. Simon (Editor); Jr., Alfred H. Merrill (Editor); Yusuf A. Hannun (Editor)
Publisher: Academic Press
Published: October 15, 2000
Language: eng
Number of pages: 648
ISBN-13: 9780121822132
First sentence: Free sphingoid bases are relatively easy to analyze because they can be extracted in high yield using standard organic solvents and, once glycerolipids are removed by base treatment, there are relatively few contaminants that elute on reversed-phase HPLC in the vicinity of the o-phthalaldehyde derivatives of most naturally occurring sphingoid bases.