Lipid signaling protocols
By: Banafshé Larijani, editor ; Rudiger Woscholski, editor ; Colin A. Rosser, editor
Series: Methods in molecular biology -- 462; Methods in molecular biology (Clifton, N.J.) -- v. 462.
Publisher: Humana; Springer, distributor]
Published: 2009
Place of publication: New York, N.Y; [London
Language: eng
Number of pages: 429
ISBN-13: 9781588297273; 9781603271158
Description: 'Lipid Signaling Protocols' assembles in a single volume the various tools and methodologies needed by the interested investigator to unravel lipid dependent signaling and cell function.
Notes: Includes bibliographical references and index.
Table of contents: title: Eicosanoids : generation and detection in mammalian cells / Valerie B. O'Donnell, Ben Maskrey, and Graham W. Taylor; title: Lipidomic analysis of phospholipids and related structures by liquid chromatography-mass spectrometry / Trevor R. Pettitt; title: Measurement of polyphosphoinositides in cultured mammalian cells / Frank T. Cooke; title: Inositol lipid-dependent functions in Saccharomyces cerevisiae : analysis of phosphatidylinositol phosphates / Stephen K. Dove and Robert H. Michell; title: Methods for the determination of the masss of nuclear PtdIns4P, PtdIns5P, and PtdIns(4,5)P₂ / David R. Jones ... [et al.]; title: Lipid quantification and structure determination of nuclear envelope precursor membranes in the sea urchin / Marie Garnier-Lhomme ... [et al.]; title: Solution and solid-state NMR of lipids / Axelle Grelard ... [et al.]; title: Spatial localization of PtdInsP₂ in phase-separated giant unilamellar vesicles with a fluorescent PLC-delta 1 PH domain / Xavier Mulet ... [et al.]; title: Studying cell-to-cell interactions : an easy method of tethering ligands on artificial membranes / Sebastian J. Fleire and Facundo D. Batista; title: Ceramide-induced transbilayer (flip-flop) lipid movement in membranes / F.-Xabier Contreras ... [et al.]; title: Effects of sterols on the development and aging of Caenorhabditis elegans / Eun-Young Lee ... [et al.]; title: Proteomic analysis of the sterol-mediated signaling pathway in Caenorhabditis elegans / Byung-Kwon Choi ... [et al.]; title: Sudying the subcellular localization and DNA-binding activity of FoxO transcription factors, downstream effectors of P13K/Akt / Abdelkader Essafi ... [et al.]; title: Measurement of PTEN activity in vivo by imaging phosphorylated Akt / Erika Rosivatz and Rudiger Woscholski; title: Analysis of phosphatidylinositol 3,4,5 trisphosphate 5-phosphatase activity by in vitro and in vivo assays / Lisa M. Ooms ... [et al.]; title: Measuring phospholipase D activity in insulin-secreting pancreatic [beta]-cells and insulin-responsive muscle cells and adipocytes / Rosanna Cazzolli ... [et al.]; title: Protein kinase C as an effector of lipid-derived second messengers / Marie-Hélène Paclet ... [et al.]; title: Detection of myotubularin phosphatases activity on phosphoinositides in vitro and ex vivo / Holger Maria Rohde ... [et al.]; title: Quantification of multiple phosphatidylinositol 4-kinase isozyme activities in cell extracts / Mark G. Waugh, Shane Minogue, and J. Justin Hsuan; title: Phospholipid-interacting proteins by solution-state NMR spectroscopy / Keiichiro Kami, Sundaresan Rajesh, and Michael Overduin; title: Revealing signaling in single cells by single- and two-photon fluorescence lifetime imaging microscopy / Damien Alcor, Veronique Calleja, and Banafshé Larijani; title: Phosphoinositide (PI) 3-kinase assays / Michael J. Fry; title: Measurement of phosphatidylinositol and phosphatidylcholine binding and transfer activity of the lipid transport Protein PITP / Shamshad Cockcroft; title: In vitro reconstitution of activation of PLC[epsilon] by Ras and Rho GTPases / Natalia Lamuño Gandarillas ... [et al.]; title: Assaying endogenous phosphatidylinositol-4-phosphate 5-kinase (PIP5K) activities / Jonathon R. Halstead ... [et al.]; title: Preparation of membrane rafts / Mark G. Waugh and J. Justin Hsuan.