Prion protein protocols
By: Andrew F. Hill, editor.
Contributors: Hill, A. F.
Series: Methods in molecular biology -- 459
Publisher: Humana
Published: 2008
Place of publication: Totowa, N.J
Language: eng
Number of pages: 280
ISBN-13: 9781588298973; 9781597452342
Subjects: Prions; Prions -- pathogenicity -- Laboratory Manuals; Prions -- isolation & purification -- Laboratory Manuals
Genres: Laboratory Manuals.
Notes: Includes bibliographical references and index.
Table of contents: title: Cell culture models to unravel prion protein function and aberrancies in prion diseases / Katarina Bedecs; title: Investigation of PrPc metabolism and function in live cells : methods for studying individual cells and cell populations / Cathryn L. Haigh and David R. Brown; title: Immunodetection of PrPsc using western and slot blotting techniques / Hanny Gyllberg and Kajsa Löfgren; title: Assaying prions in cell culture : the standard scrapie cell assay (SSCA) and the scrapie cell assay in end point format (SCEPA) / Sukhvir P. Mahal ... [et al.]; title: Generation of cell lines propagating infectious prions and the isolation and characterization of cell-derived exosomes / Laura J. Vella and Andrew F. Hill; title: Neurotoxicity of prion peptides on cultured cerebellar neurons / Giuseppe D. Ciccotosto, Roberto Cappai, and Anthony R. White; title: Understanding the nature of prion diseases using cell-free assays / Victoria A. Lawson; title: Methods for conversion of prion protein into amyloid fibrils / Leonid Breydo, Natallia Makarava, and Ilia V. Baskakov; title: Amplification of purified prions in vitro / Surachai Supattapone, Nathan R. Deleault, and Judy R. Rees; title: Expression and purification of full-length recombinant PrP of high purity / Natallia Makarava and Ilia V. Baskakov; title: Analysis of PrP conformation using circular dichroism / Sen Han and Andrew F. Hill; title: Effect of copper on the de novo generation of prion protein expressed in Pichia pastoris / Carina Treiber; title: Biophysical investigations of the prion protein using electron paramagnetic resonance / Simon C. Drew and Kevin J. Barnham; title: Molecular diagnosis of human prion disease / Jonathan D.F. Wadsworth ... [et al.]; title: Analysis of endogenous PrPc processing in neuronal and non-neuronal cell lines / Victoria Lewis and Steven J. Collins; title: Molecular typing of PrPres in human sporadic CJD brain tissue / Victoria Lewis ... [et al.]; title: Transgenic mouse models of prion diseases / Glenn C. Telling; title: Quantitiative bioassay of surface-bound prion infectivity / Victoria A. Lawson.