Therapeutic peptides
Subtitle: methods and protocols
By: edited by Andrew E. Nixon, Discovery Research, Dyax Corp, Cambridge, NA, USA
Series: Methods in molecular biology -- v. 1088; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 1088.; Springer protocols (Series)
Publisher: Humana Press
Published: 2014
Place of publication: New York
Language: eng
Number of pages: 279
ISBN-13: 9781627036726; 9781627036733
Copyright date: 2014
Subjects: Peptides; Laboratory manuals; Therapeutic use; Laboratory Manuals
Description: Therapeutic Peptides: Methods and Protocols features biological methods for the preparation of peptide phage display libraries using both filamentous and lytic phage. With contributions from renowned authors in the field, the book also explores selection and screening of the prepared peptide libraries for peptides with the desired function and the subsequent characterization of the identified peptides. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and dependable, Therapeutic Peptides: Methods and Protocols is an ideal guide for researchers from all backgrounds seeking methods for the identification of therapeutic peptide candidates.
Notes: Includes bibliographical references and index.
Table of contents: title: Design of phage-displayed cystine-stabilized mini-protein libraries for proteinaceous binder engineering  -- Hung-Ju Chang and An-Suei Yang; title: Construction of a filamentous phage display peptide library  -- Annette Fagerlund, Astrid Hilde Myrset, and Mari Ann Kulseth; title: Engineering bioactive peptide-based therapeutic molecules  -- Jong Sang Ryu [and three others]; title: T7 lytic phage-displayed peptide libraries : construction and diversity characterization  -- Lauren R.H. Krumpe and Toshiyuki Mori; title: Affinity selection using filamentous phage display  -- Mari Ann Kulseth, Annette Fagerlund, and Astrid Hilde Myrset; title: Bioprospecting open reading frames for peptide effectors  -- Ling Xiong and Charles Scott; title: Identification of ideal peptides for heterovalent ligands  -- Ajay Shrivastava [and three others]; title: Substrate phage display for protease substrate sequence characterization : bovine factor Xa as a model system  -- Hung-Ju Hsu and An-Suei Yang; title: Engineering peptide therapeutics using MIMETIBODY [trademark] technology  -- Kristen Picha [and three others]; title: Evaluation of peptides as protease inhibitors and stimulators  -- Hannu Koistinen [and four others]; title: Assessment of antimicrobial (host defense) peptides as anti-cancer agents  -- Susan Douglas, David W. Hoskin, and Ashley L. Hilchie; title: Peptide labelling strategies for imaging agents  -- Tuulia Huhtal [and three others]; title: Peptide optimization and conjugation strategies in the development of molecularly targeted magnetic resonance imaging contrast agents  -- Andrew F. Kolodziej [and four others]; title: Evaluation of prenylated peptides for use in cellular imaging and biochemical analysis  -- Joshua D. Ochocki [and five others]; title: Ultraviolet absorption spectroscopy of peptides  -- Mangala R. Liyanage [and three others]; title: Fluorescence spectroscopy of peptides  -- Mangala R. Liyanage [and three others]; title: Circular dichroism of peptides  -- Kunal Bakshi [and three others]; title: Fourier transform infrared spectroscopy of peptides -- Kunal Bakshi [and three others].