Cilia
Subtitle: model organisms and intraflagellar transport
By: edited by Stephen M. King and Gregory J. Pazour
Series: Methods in cell biology -- v. 93; Methods in cell biology -- v. 93.
Publisher: Elsevier Academic Press
Published: 2009
Place of publication: Burlington, MA
Language: eng
Format: [electronic resource] :
Number of pages: 390
ISBN-13: 9780123813787; 9780123813770
Subjects: Life Sciences; Cilia; Biological Transport; Flagella (Microbiology); Science; Cell membranes; Cilia and ciliary motion; SCIENCE; Natural history; Cytology; Cell Movement
Description: "Cilia are highly conserved organelles that serve motile and/or sensory functions. These organelles power cell movement, generate fluid flow in various organs, act as sensors of the extracellular environment and have been modified for various specialized tasks such as light reception and smell. Defects in these ubiquitous organelles lead to a broad array of human genetic disorders that range from polycystic kidney disease, retinal degeneration, epilepsy and infertility to developmental defects such as situs inversus and polydactyly. This volume is the third in a three-part series on cilia that focuses on the use of model organisms to gain insight into ciliary function and on the process of intraflagellar transport (IFT) that is essential for the assembly and maintenance of ciliary structures."--Publisher's description.
Notes: Includes bibliographical references and index.

Description based on print version record.
Table of contents: title: Manipulating ciliary protein-encoding genes in Tetrahymena thermophila / Drashti Dave, Dorota Wloga, Jacek Gaertig.; title: Approaches for functional analysis of flagellar proteins in African trypanosomes / Michael Oberholzer, Miquel A. Lopez, Katherine S. Ralston, Kent L. Hill.; title: Tools for analyzing intraflagellar transport in trypanosomes / Daria Julkowska, Philippe Bastin.; title: Schmidtea mediterranea: a model system for analysis of motile cilia / Panteleimon Rompolas, Ramila S. Patel-King, Stephen M. King.; title: Targeted gene silencing by RNA interference in Chlamydomonas / Eun-Jeong Kim, Heriberto Cerutti.; title: Analysis of cargo transport by IFT and GFP imaging of IFT in Chlamydomonas / Dennis Diener.; title: Genetic and phenotypic analysis of flagellar assembly mutants in Chlamydomonas reinhardtii / Carlo Iomini, Jacob E. Till, Susan K. Dutcher.; title: Recording and analyzing IFT in Chlamydomonas flagella / William Dentler, Kristyn VaderWaal, Mary E. Porter.; title: Total internal reflection fluorescence (TIRF) microscopy of Chlamydomonas flagella / Benjamin D. Engel, Karl-Ferdinand Lechtreck, Tsuyoshi Sakai, Mitsuo Ikebe, George B. Witman, Wallace F. Marshall.; title: Purification of IFT particle proteins and preparation of recombinant proteins for structural and functional analysis / Robert H. Behal, Ewelina Betleja, Douglas G. Cole.; title: Studying cilia in Zebrafish / Iain Drummond.; title: Analysis if IFT kinesins in developing Zebrafish cone photoreceptor sensory cilia / Christine Insinna, Katherine Luby-Phelps, Brian A. Link, Joseph C. Besharse.; title: Analysis of intraflagellar transport in C. elegans sensory cilia / Limin Hao, Seyda Acar, James Evans, Guangshuo Ou, Jonathan M. Scholey.; title: Functional genomics of intraflagellar transport-associated proteins in C. Elgans.; title: Generating conditional mutants to analyze ciliary functions: the use of cre-lox technology to disrupt cilia in specific organs / Amber K. O'Connor, Robert A. Kesterson, Bradley K. Yoder.; title: Imaging intraflagellar transport in mammalian primary cilia / Tatiana Y. Besschetnova, Barnali Roy, Jagesh V. Shah.; title: Analysis of hedgehog signaling in mouse intraflagellar transport mutants / Hyuk W. Ko, Aimin Liu, Jonathan T. Eggenschwiler.