Photoswitching proteins
Subtitle: methods and protocols
By: edited by Sidney Cambridge, Institute of Anatomy and Cell Biology, University of Heidelberg, Germany
Series: Methods in molecular biology -- 1148; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- 1148.; Springer protocols (Series)
Publisher: Humana Press; Springer
Published: 2014
Place of publication: New York
Language: eng
Number of pages: 278
ISBN-13: 9781493904693; 9781493904709
Copyright date: 2014
Subjects: Fluorescence; Optical Imaging; Photochromism; Proteins; Photochemical Processes; Laboratory manuals; Laboratory Manuals; Optogenetics
Description: "... focuses on proven applications of photoswitching proteins in cell and neurobiology. Optical approaches have been mostly embraced by the neurosciences to allow fast, high-resolution characterization and manipulation of single cells in complex neuronal tissue. These tools, including photoswitching of proteins, are now successfully used in almost all fields of biological research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Photoswitching Proteins: Methods and Protocols highlights many of the possibilities for research based on light-inducible, high-resolution manipulation of biological systems."--P. [4] of cover. --
Notes: Includes bibliographical references and index.
Table of contents: title: Photoinduced damage resulting from fluorescence imaging of live cells  -- Matthew K. Daddysman, Michael A. Tycon, and Christopher J. Fecko; title: Modification of purified proteins with photochemical protection compounds for high-resolution photoactivation of protein function in vitro and in vivo  -- Sidney Cambridge; title: Optochemical activation of kinase function in live cells  -- Andrei V. Karginov, Klaus M. Hahn, and Alexander Deiters; title: Photoswitching of cell surface receptors using tethered ligands  -- Andreas Reiner and Ehud Y. Isacoff; title: Photocontrol of AMPA receptors with a photochromic ligand  -- Martin Sumser and Philipp Stawski; title: Photoconversion of CFP to study neuronal tissue with electron microscopy  -- Nina Wittenmayer; title: Light-inducible gene regulation with engineered zinc finger proteins  -- Lauren R. Polstein and Charles A. Gersbach; title: Manipulation of plasma membrane phosphoinositides using photoinduced protein-protein interactions  -- Olof Idevall-Hagren and Pietro DeCamilli; title: Roadmap to applying optogenetics in neuroscience  -- Consuelo Fois, Pierre-Hugues Prouvot, and Albrecht Stroh; title: Salvaging ruins : reverting blind retinas into functional visual sensors  -- Marion Mutter, Natalia Swietek, and Thomas A. Münch; title: Photoactivated adenylyl cyclases as optogenetic modulators of neuronal activity  -- Wagner Steuer Costa, Jana Liewald, and Alexander Gottschalk; title: Structural basis of photoswitching in fluorescent proteins  -- Chenxi Duan ... [et al.]; title: Using photoactivatable GFP to track axonal transport kinetics  -- Archan Ganguly and Subhojit Roy; title: In vivo cell tracking using PhOTO zebrafish  -- William P. Dempsey, Hanyu Qin, and Periklis Pantazis; title: In vivo optogenetics for light-induced oxidative stress in transgenic zebrafish expressing the killerred photosensitizer protein  -- Cathleen Teh and Vladimir Korzh; title: Photoactivatable fluorescent proteins for super-resolution microscopy  -- Yuji Ishitsuka, Karin Nienhaus, and G. Ulrich Nienhaus; title: pcSOFI as a smart label-based superresolution microscopy technique -- Benjamien Moeyaert and Peter Dedecker.