PCR
Subtitle: the Basics
Contributors: Moller, Simon
Edition: 2nd ed.
Series: Basics (Bio Methods); Basics (Bio Methods)
Publisher: Taylor & Francis Ltd.
Published: 2006
Place of publication: Hoboken
Language: eng
Number of pages: 305
ISBN-13: 9780203002674
Subjects: Polymerase chain reaction; DNA Replication; Gene amplification; Réaction en chaîne de la polymérase
Description: A thoroughly updated version of the successful first edition, with a new chapter on Real-Time PCR, more prokaryotic applications, and more detail in the complex mutagenesis sections.
Table of contents: title: Book Cover; Title; Copyright; Contents; Abbreviations; Preface; 1 An introduction to PCR; 2 Understanding PCR; 3 Reagents and instrumentation; 4 Optimization of PCR; 5 Analysis, sequencing and in vitro expression of PCR products; 6 Purification and cloning of PCR products; 7 PCR mutagenesis; 8 Analysis of gene expression; 9 Real-time RT-PCR; 10 Cloning genes by PCR; 11 Genome analysis; Index.