PTEN suppression-mediated neuroprotection in ischemic neuronal injury.
Published: 2006
Language: eng
Number of pages: 87
ISBN-13: 9780494213704
Description: The tumor suppressor PTEN is a lipid and protein phosphatase that has been shown to play a key role in regulating diverse molecular events of mammalian cells. In the context of ischemic damage, there is evidence that suppressing PTEN activity brings about neuroprotective effects, yet the underlying mechanisms of this protection remains unclear. Recently, a novel PTEN inhibitor, bpV(pic), has been shown to preferentially inhibit PTEN at nanomolar concentrations in non-neuronal cells. As an important first step for further elucidation of PTEN signaling pathways in the CNS, I hypothesized that BpV(pic) treatment produces PTEN suppression-mediated neuroprotection in rat cortical cells. My research showed that treatment with 200 nM bpV(pic) (1) enhanced the phosphorylation of Akt in vitro and in vivo, (2) did not inhibit protein tyrosine phosphatases at nanomolar concentrations, (3) protected against oxygen-glucose deprivation-induced injury. Additionally, preliminary data showed that BpV(pic) treatment reduced the surface expression of GABA AR-gamma2 subunits.
Notes: Source: Masters Abstracts International, Volume: 45-03, page: 1395.

Thesis (M.Sc.)--University of Toronto, 2006.

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