Microtubule dynamics
Subtitle: methods and protocols
By: edited by Anne Straube
Series: Methods in molecular biology -- 777; Springer protocols
Publisher: Humana; Springer
Published: 2011
Place of publication: New York
Language: eng
Number of pages: 319
ISBN-13: 9781617792519; 9781617792526
Subjects: Methodology; Laboratory Manuals; Microtubules; Research
Notes: Includes bibliographical references and index.
Table of contents: title: How to measure microtubule dynamics  -- Anne Straube; title: Purification of tubulin from porcine brain  -- Christopher Gell ... [et al.]; title: Purification of tubulin from the fission yeast Schizosaccharomyces pombe  -- Douglas R. Drummond ... [et al.]; title: Generation of differentially polyglutamylated microtubules  -- Benjamin Lacroix and Carsten Janke; title: Preparation of pure tyrosinated or detyrosinated tubulin isoforms  -- Laurence Lafanechere and Didier Job; title: ab-tubulin and microtubule-binding assays  -- Jaime N. Campbell and Kevin C. Slep; title: Analysis of microtubule assembly kinetics using turbidimetry  -- Felicia Gaskin; title: Isolation of centrosomes from cultured cells  -- Simone Reber; title: Preparation of dual-color polarity-marked fluorescent microtubule seeds  -- Miho Katsuki, Etsuko Muto, and Robert A. Cross; title: Reconstitution and quantification of dynamic microtubule end tracking in vitro using TIRF microscopy -- Ivo A. Telley, Peter Bieling, and Thomas Surrey; title: ^; title: Force generation by dynamic microtubules in vitro  -- Svenja-Marei Kalisch, Liedewij Laan, and Marileen Dogterom; title: Variations on the single-molecule assay for microtubule-associated proteins and kinesins  -- Susanne Bechstedt ... [et al.]; title: Analysing the ATP turnover cycle of microtubule motors  -- Claire T. Friel, Clive R. Bagshaw, and Jonathon Howard; title: Cryo-electron tomography of microtubules assembled in vitro from purified components  -- Frederic M. Coquelle ... [et al.]; title: Kinetochore microtubules, analyzed by correlated light and immunoelectron microscopy  -- Andreas Merdes and Jan De Mey; title: Photoactivatable-GFP-a-tubulin as a tool to study microtubule plus-end turnover in living human cells  -- Catarina P. Samora and Andrew D. McAinsh; title: Quantification of asymmetric microtubule nucleation at subcellular structures  -- Xiaodong Zhu and Irina Kaverina; title: Investigating microtubule dynamic instability using microtubule-targeting agents ; title: ^ -- Stephane Honore and Diane Braguer; title: Laser ablation of the microtubule cytoskeleton : setting up and working with an ablation system  -- Nicola Maghelli and Iva M. Tolic-Norrelykke; title: Isolation, identification, and validation of microtubule-associated proteins from Drosophila embryos  -- Robin Antrobus and James G. Wakefield; title: Isolation of novel +TIPs and their binding partners using affinity purification techniques -- Ka Lou Yu ... [et al.].