RNAi and plant gene function analysis
Subtitle: methods and protocols
By: edited by Hiroaki Kodama, Atsushi Komamine
Series: Springer protocols; Methods in molecular biology -- v. 744; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 744.
Publisher: Springer
Published: 2011
Place of publication: New York
Language: eng
Number of pages: 244
ISBN-13: 9781617791222; 9781617791239
Subjects: Plants; Small interfering RNA; Plant genetics; Genetics; Laboratory Manuals; RNA Interference; Laboratory manuals
Notes: Includes bibliographical references and index.
Table of contents: title: Overview of plant RNAi  -- Yuichiro Watanabe; title: Caveat of RNAi in plants : the off-target effect  -- Muthappa Senthil-Kumar and Kirankumar S. Mysore; title: Plant gateway vectors for RNAi as a tool for functional genomic studies  -- Toshiya Muranaka; title: Heat-inducible RNAi for gene functional analysis in plants  -- Frederic Masclaux and Jean-Philippe Galaud; title: Gene function analysis by artificial microRNAs in Physcomitrella patens  -- Basel Khraiwesh ... [et al.] -- Virus-induced gene silencing in ornamental plants / -- Cai-Zhong Jiang, Jen-Chih Chen, and Michael Reid; title: Local RNA silencing mediated by agroinfiltration  -- Jutta Maria Helm, Elena Dadami, and Kriton Kalantidis; title: Direct transfer of synthetic double-stranded RNA into protoplasts of Arabidopsis thaliana  -- Ha-il Jung, Zhiyang Zhai, and Olena K. Vatamaniuk; title: Detection of long and short double-stranded RNAs  -- Toshiyuki Fukuhara ... [ et al.]; title: Quantitative stem-loop RT-PCR for detection of microRNAs  -- Erika Varkonyi-Gasic and Roger P. Hellens; title: Large-scale sequencing of plant small RNAs  -- William P. Donovan, Yuanji Zhang, and Miya D. Howell; title: Computational prediction of plant miRNA targets  -- Ying-Hsuan Sun ... [et al.]; title: Bisulfite sequencing for cytosine-methylation analysis in plants  -- Nazmul Haque and Masamichi Nishiguchi; title: Using nuclear run-on transcription assays in RNAi studies  -- Basel Khraiwesh; title: Proteomic analysis of RNA interference induced knockdown plant  -- Sang Yeol Lee and Kyun Oh Lee; title: Comparative analysis of phosphoprotein expression using 2D-DIGE -- Tomoya Asano and Takumi Nishiuchi.