Cytotoxicity assessment of components of the Epiphany resin-percha obturating system by indirect and direct contact Millipore filter assays.
Published: 2006
Language: eng
Number of pages: 86
ISBN-13: 9780494211007
Description: The Epiphany System was assessed for cytotoxicity, compared to gutta-percha and AH-Plus sealer. Specimen disks (Resilon, gutta-percha), filled glass rings (sealers), or imbibed cellulose disks (primer, thinning resin) were placed over Millipore filters in direct or indirect contact with a HeLa cell monolayer, incubated for 2h and stained with tetrazolium blue. Cytotoxicity was rated by the surrounding unstained zone: none (0mm), mild (&le; 7mm), moderate (7-12mm), or marked (>12mm). Data were analyzed with 1-way ANOVA and post-hoc pairwise t-tests. Unstained zones indicating moderate cytotoxicity were significantly larger (p&lt;0.05) for Epiphany primer than for thinning resin and for freshly mixed AH-Plus than for Epiphany sealer. Set sealers (24, 48h), gutta-percha and Resilon elicited non-cytotoxic responses. In conclusion, cytotoxicity of set Epiphany sealer and Resilon was comparable to that of set AH-Plus and gutta-percha. Cytotoxicity of freshly mixed Epiphany sealer, primer, and thinning resin did not exceed that of freshly mixed AH-Plus.
Notes: Source: Masters Abstracts International, Volume: 45-03, page: 1442.

Thesis (M.Sc.)--University of Toronto, 2006.

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