Plant metabolic flux analysis
Subtitle: methods and protocols
By: edited by Martine Dieuaide-Noubhani, INRA Bordeaux, Université Bordeaux, Villenave d'Ornon, France, Ana Paula Alonso, Department of Molecular Genetics, The Ohio State University, Columbus, OH, USA
Contributors: Springer Science+Business Media
Series: Methods in molecular biology -- 1090; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 1090,; Springer protocols (Series)
Publisher: Humana Press
Published: 2014
Place of publication: New York
Language: eng
Number of pages: 365
ISBN-13: 9781627036870; 9781627036887
Copyright date: 2014
Subjects: Plant metabolites; Research; Plant molecular biology; Methodology; Plants; Laboratory Manuals; Metabolism; Metabolic Flux Analysis; Methods
Notes: Includes bibliographical references and index.
Table of contents: title: Application of Metabolic Flux Analysis to Plants; title: Metabolic Network Reconstruction and Their Topological Analysis; title: 14C pulse Labeling to Estimate External Fluxes and Turnovers in Primary Metabolism; title: Optimization of Steady-state 13C-labeling Experiments for Metabolic Flux Analysis; title: Quantification of 13C Enrichments and Isotopomer Abundances for Metabolic Flux analysis using 1D NMR Spectroscopy; title: Analysis of Proteinogenic Amino Acid and Starch Labeling by 2D NMR; title: Analysis of Kinetic Labeling of Amino Acids and Organic Acids by GC-MS; title: Quantifying 13C-labeling in Free Sugars and Starch by GC-MS; title: Liquid Chromatography Tandem Mass Spectrometry for Measuring 13C-labeling in Intermediates of the Glycolysis and Pentose-phosphate Pathway; title: In Vivo NMR for 13C metabolic? Flux Analysis; title: Steady State and Instationary Modeling of Proteinogenic and Free Amino Acid Isotopomers for Flux Quantification; title: Isotopically Nonstationary MFA (INST-MFA) of Autotrophic Metabolism; title: Simulating Labeling to Estimate Kinetic Parameters for Flux Control Analysis; title: High-throughput Data Pipelines for Metabolic Flux Analysis in Plants; title: Analysis of Enzyme Activities; title: Analytical Kinetic Modeling: A Practical Procedure; title: Flux Balance Analysis as an Alternative Method to Estimate Fluxes Without Labeling; title: Flux Variability Analysis: Application to Developing Oilseed Rape Embryos using Toolboxes for Constraint-Based Modeling; title: Plant Genome-Scale Modeling and Implementation; title: 34S and 15N Labelling to Model S and N Flux in Plants and Determine the Different Components of N and S use Efficiency; title: Ecophysiological Process-based Model to Simulate Carbon Fluxes in Plants.