The Soh1 (Med31) subunit of the Saccharomyces cerevisiae  mediator complex.
Published: 2007
Language: eng
Number of pages: 71
ISBN-13: 9780494274873
Description: Soh1(Med31), a human Mediator complex subunit, is also a component of the Saccharomyces cerevisiae Mediator complex, which serves as a transcriptional coactivator for RNA polymerase II in eukaryotes. In this thesis, I show by chromatin immunoprecipitation(ChIP) that Soh1(Med31) is recruited to the galactose-induced GAL1-10 promoter region.  SOH1(MED31) and the genes encoding Mediator head module subunits Srb2(Med20) and Srb5(Med18), in addition to Spt3 from the SAGA complex, display similar patterns of synthetic genetic interactions with other genes involved in transcription. Hierarchical clustering of non-essential Mediator subunit genes according to their degree of similarity in synthetic genetic interactions and effects on gene expression in microarray experiments predicts that Soh1(Med31) is a component of the head module of Mediator. However, affinity purification of TAP-tagged Soh1(Med31) and Srb4(Med17) with IgG antibody in conjunction with urea-mediated dissociation of Mediator subunits and western blot analysis suggests that Soh1(Med31) is not a Mediator head domain subunit.
Notes: Source: Masters Abstracts International, Volume: 45-06, page: 3061.

Thesis (M.Sc.)--University of Toronto, 2007.

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