Characterization of the transient receptor potential channels mediating lysophosphatidic acid-stimulated calcium mobilization in B lymphoblasts.
Published: 2006
Language: eng
Number of pages: 109
ISBN-13: 9780494161920
Description: Altered 1-oleoyl-lysophosphatidic acid (LPA, 100 muM)-stimulated calcium responses occur in B-lymphoblast cell lines from bipolar disorder patients, but the mechanism(s) involved is uncertain. LPA shares a structurally similar fatty acid side chain with the diacylglycerol analogue, 1-oleoyl-2-acetyl-sn-glycerol (OAG), a known activator of subtypes 3, 6 and 7 of the canonical transient receptor potential (TRPC) cation channel subfamily. Accordingly, the objective of this study was to determine whether the LPA-stimulated calcium response in B-lymphoblasts is mediated, in part, through this TRPC channel subfamily. Divalent cation selectivity in response to thapsigargin, LPA and OAG was used to distinguish TRPC-like character from store-operated channels. The results suggest that 100 muM LPA stimulates calcium entry through channels with characteristics similar to TRPC3/6/7. The findings of enhanced LPA-stimulated calcium responses from bipolar disorder compared with healthy subjects may implicate dysfunction of TRPC3-like calcium entry in the pathophysiology of bipolar disorder.
Notes: Source: Masters Abstracts International, Volume: 44-06, page: 2777.

Thesis (M.Sc.)--University of Toronto, 2006.

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