DNA Sequencing Protocols (Methods in Molecular Biology)
Contributors: Colin A Graham (Editor); Alison J.M. Hill (Editor)
Edition: 2nd edition
Publisher: Humana Press
Published: January 15, 2001
Language: eng
Format: Paperback
Number of pages: 244
ISBN-13: 9780896037212
Subjects: DNA; Molecular biology; Life Sciences - Genetics & Genomics; Science; Science/Mathematics; Life Sciences - Biochemistry; Life Sciences - Biology - Molecular Biology; Science / Cytology
First sentence: DNA sequencing methods were first developed more than 20 years ago with the publication of two approaches to sequencing methodology that became known as Sanger sequencing (1), based on enzymatic synthesis from a single-stranded DNA template with chain termination using dideoxyucleotides (ddNTPs) and Maxim-Gilbert sequencing (2), which involved chemical degradation of end-radiolabeled DNA fragments.