Electron microscopy
Subtitle: methods and protocols
Contributors: Kuo, John
Edition: 2nd ed. / edited by John Kuo
Series: Methods in molecular biology -- 369; Methods in molecular biology (Clifton, N.J.) --  369
Publisher: Humana Press
Published: 2007
Place of publication: Totowa, N.J
Language: eng
Number of pages: 608
ISBN-13: 9781588295736
Subjects: Electron microscopy -- Laboratory manuals; Histology -- Technique
Genres: Laboratory manuals
Other titles: Electron microscopy methods and protocols
Description: Comprises two major areas of electron microscopy-transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The TEM area covers several key techniques, including: conventional specimen preparation methods for cultured cells and biomedical and plant tissues; cryospecimen preparation by high-pressure freezing and cryoultramicrotomy negative staining and immunogold labeling techniques; and TEM crystallography and cryo-TEM tomography. The SEM area similarly attends to conventional-, variable pressure-, environmental-, and cryoscanning microscopy techniques, as well as the application of X-ray microanalysis. Protocols for the application of X-ray microanalysis to SEM and mass spectrometry conclude the volume.
Notes: Rev. ed. of: Electron microscopy methods and protocols / edited by M.A. Nasser Hajibagheri. c1999

Includes bibliographical references and index
Table of contents: title: Conventional specimen preparation techniques for transmission electron microscopy of cultured cells / John J. Bozzola; title: Processing biological tissues for ultrastructural study / José A. Mascorro and John J. Bozzola; title: Processing plant tissues for ultrastructural study / John Kuo; title: Microwave-assisted processing and embedding for transmission electron microscopy / Paul Webster; title: Ultramicrotomy for biological electron microscopy / Herbert K. Hagler; title: Poststaining grids for transmission electron microscopy : conventional and alternative protocols / E. Ann Ellis; title: Negative staining of thinly spread biological samples / J. Robin Harris; title: Recent advances in high-pressure freezing : equipment- and specimen-loading methods / Kent L. McDonald ... [et al.]; title: Cryoultramicrotomy : cryoelectron microscopy of vitreous sections / Dimitri Vanhecke, Luca Studer, and Daniel Studer; title: Cell-free extract systems and the cytoskeleton : preparation of biochemical experiments for transmission electron microscopy / Margaret Coughlin, William M. Brieher, and Ryoma Ohi; title: Electron microscopy in situ hybridization : tracking of DNA and RNA sequences at high resolution / Dušan Cmarko and Karel Koberna; title: Correlative light and electron microscopy using immunolabeled resin sections / Heinz Schwarz and Bruno M. Humbel; title: Cryosectioning fixed and cryoprotected biological material for immunocytochemistry / Paul Webster and Alexandre Webster; title: Fluorescence-integrated transmission electron microscopy images : integrating fluorescence microscopy with transmission electron microscopy / Paul A. Sims and Jeff D. Hardin; title: Quantitative immunoelectron microscopy : alternative ways of assessing subcellular patterns of gold labelling / Terry M. Mayhew; title: Electron crystallography of membrane proteins / Hui-Ting Chou, James E. Evans, and Henning Stahlberg; title: Cryoelectron microscopy of icosahedral virus particles / Wen Jiang and Wah Chiu; title: Three-dimensional reconstruction of chromosomes using electron tomography / Peter Engelhardt; title: Whole-mount immunoelectron tomography of chromosomes and cells / Peter Engelhardt ... [et al.]; title: Three-dimensional cryotransmission electron microscopy of cells and organelles / Michael Marko and Chyong-Ere Hsieh; title: Electron energy-loss spectroscopy as a tool for elemental analysis in biological specimens / Nadine Kapp ... [et al.]; title: Conventional specimen preparation techniques for scanning electron microscopy of biological specimens / John J. Bozzola; title: Variable pressure and environmental scanning electron microscopy : imaging of biological samples / Brendan J. Griffin; title: Cryoplaning technique for visualizing the distribution of water in woody tissues by cryoscanning electron microscopy / Yasuhiro Utsumi and Yuzou Sano; title: X-ray microanalysis in the scanning electron microscope / Godfrield M. Roomans and Anca Dragomir; title: Botanical X-ray microanalysis in cryoscanning electron microscopy / Beat Frey; title: Static secondary ion mass spectrometry for biological and biomedical research / Nicholas P. Lockyer; title: Using SIMS and MIMS in biological materials : application to higher plants / Nicole Grignon