Argonaute proteins
Subtitle: methods and protocols
By: edited by Tom C. Hobman, Thomas F. Duchaine
Series: Methods in molecular biology -- 725; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 725.; Springer protocols
Publisher: Humana Press/Springer
Published: 2011
Place of publication: New York
Language: eng
Number of pages: 344
ISBN-13: 9781617790454; 9781617790461
Subjects: RNA
Notes: Includes bibliographical references and index.
Table of contents: title: Purification of native argonaute complexes from the fission yeast Schizosaccharomyces pombe  -- Shane M. Buker and Mohammad R. Motamedi; title: Chromatin immunoprecipitation in fission yeast  -- Thomas A. Volpe and Jessica DeMaio; title: Biochemical analyzes of endogenous argonaute complexes immunopurified with anti-argonaute monoclonal antibodies  -- Keita Miyoshi ... [et al.]; title: Mapping of Ago2-GW182 functional interactions   -- Bing Yao ... [et al.]; title: Continuous density gradients to study argonaute and GW182 complexes associated with the endocytic pathway  -- Derrick Gibbings; title: In vitro RISC cleavage assay  -- Julia Stoehr and Gunter Meister; title: Native gel analysis for RISC assembly  -- Tomoko Kawamata and Yukihide Tomari; title: Purification and assembly of human argonaute, dicer, and TRBP complexes  -- Nabanita De and Ian J. MacRae; title: Detection of human dicer and argonaute 2 catalytic activity  -- Marjorie P. Perron ... [et al.]; title: Imaging the cellular dynamics of drosophila argonaute proteins  -- Jing Li ... [et al.]; title: Live Cell imaging of argonaute proteins in mammalian cells  -- Justin M. Pare, Joaquin Lopez-Orozco, and Tom C. Hobman; title: Reporter-based assays for analyzing RNA interference in mammalian cells  -- Lydia V. McClure, Gil Ju Seo, and Christopher S. Sullivan; title: Artificial tethering of argonaute proteins for studying their role in translational repression of target mRNAs  -- Stephanie Eckhardt ... [et al.]; title: Efficient system for Let-7 microRNA and GW182 protein-mediated deadenylation in vitro  -- Marc R. Fabian, Yuri V. Svitkin, and Nahum Sonenberg; title: Cell-free microRNA-mediated translation repression in Caenorhabditis elegans  -- Edlyn Wu and Thomas F. Duchaine; title: Argonaute pull-down and RISC analysis using 2'-O -methylated oligonucleotides affinity matrices  -- Guillaume Jannot, Alejandro Vasquez-Rifo, and Martin J. Simard; title: Cloning argonaute-associated small RNAs from Caenorhabditis elegans  -- Weifeng Gu ... [et al.]; title: Immunoprecipitation of piRNPs and directional, next generation sequencing of piRNAs  -- Yohei Kirino ... [et al.]; title: Generation of an inducible mouse ES cell lines deficient for argonaute proteins  -- Hong Su and Xiaozhong Wang; title: Whole cell proteome regulation by microRNAs captured in a pulsed SILAC mass spectrometry approach -- Olivia A. Ebner and Matthias Selbach.