Phospho-proteomics
Subtitle: methods and protocols
By: edited by Marjo de Graauw
Series: Methods in molecular biology -- 527; Springer protocols
Publisher: Humana; Springer [distributor]
Published: 2009
Place of publication: New York, NY; London
Language: eng
Number of pages: 328
ISBN-13: 9781603278331; 9781603278348
Subjects: Protein kinases; Methods; Phosphoproteins; Proteomics; Laboratory Manuals; Phosphorylation
Notes: Includes bibliographical references and index.
Table of contents: title: A high-resolution two dimensional gel- and pro-Q DPS-based proteomics workflow for phosphoprotein identification and quantitative profiling / Ganesh K. Agrawal and Jay J. Thelen; title: Differential phosphoprotein labelling (DIPPL) using ³²P and ³³P / Aviva M. Tolkovsky and Andreas Wyttenbach; title: Identification of oxidative stress-induced tyrosine phosphorylated proteins by immunoprecipitation and mass spectrometry / Ma Carmen Duran, Hong-Lin Chan, and John F. Timms; title: Enrichment and characterization of phosphopeptides by immobilized metal affinity chromatography (IMAC) and mass spectrometry / Tine E. Thingholm and Ole N. Jensen; title: The use of titanium dioxide micro-columns to selectively isolate phosphopeptides from proteolytic digests / Tine E. Thingholm and Martin R. Larsen; title: Enrichment and separation of mono- and multiply phosphorylated peptides using sequential elution from IMAC prior to mass spectrometric analysis / Tine E. Thingholm , Ole N. Jensen, and Martin R. Larsen; title: ^; title: Use of stable isotope labeling by amino acids in cell culture (SILAC) for phophotyrosine protein identification and quantitation / Guoan Zhang and Thomas A. Neubert; title: Hydrophilic interaction chromatography for fractionation and enrichment of the phosphoproteome / Dean E. McNulty and Roland S. Annan; title: SILAC for global phosphoproteomic analysis / Genaro Pimienta, Raghothama Chaerkady, and Akhilesh Pandey; title: Quantitative phospho-proteomics based on soluble nanopolymers / Anton Iliuk and W. Andy Tao; title: Profiling the tyrosine phosphorylation state using SH2 domains / Kevin Dierck ... [et al.]; title: An overview of the qualitative analysis of phosphoproteins by mass spectrometry / Philip R. Gafken; title: The analysis of phosphoproteomes by selective labelling and advanced mass spectrometric techniques / Angela Amoresano ... [et al.]; title: On-line liquid chromatography electron capture dissociation for the characterization of phosphorylation sites in proteins / Steve M.M. Sweet and Helen J. Cooper; title: ^; title: ^^; title: Quantification of protein phosphorylation by [mu]LC-ICP-MS / Ralf Krüger, Nico Zinn, and Wolf D. Lehmann; title: Reverse-phase diagonal chromatography for phosphoproteome research / Kris Gevaert and Joël Vandekerckhove; title: Chemical tagging strategies for mass spectrometry-based phospho-proteomics / Alexander Leitner and Wolfgang Lindner; title: Antibody array platform to monitor protein tyrosine phosphorylation in mammalian cells / Alicia S. Chung and Y. Eugene Chin; title: Protein tyrosine kinase characterization based on fully automated synthesis of (phospho)peptide arrays in microplates / W. Carl Saxinger; title: Kinome profiling using peptide arrays in eukaryotic cells / Kaushal Parikh, Maikel P. Peppelenbosch, and Tita Ritsema; title: ProMoST : a tool for calculating the pI and molecular mass of phosphorylated and modified proteins on two-dimensional gels / Brian D. Halligan; title: Kinase-specific preiction of protein phosphorylation sites / Martin L. Miller and Nikolaj Blom; title: ^; title: ^^; title: Reconstructing regulatory kinase pathways from phosphopeptide data : a bioinformatics approach / Lawrence G. Puente, Robin E.C. Lee, and Lynn A. Megeney.; title: ^^