Design and implementation of a cross-species genetic communication circuit.
Published: 2006
Language: eng
Number of pages: 136
ISBN-13: 9780494163139
Description: A cross-species genetic communication circuit passes signals between a Gram-positive bacterium, Streptococcus mutans, and a Gram-negative bacterium, Vibrio harveyi. The signaling molecule is Autoinducer-2 (AI-2).Mathematical models were developed for the circuit. A deterministic approach was used to study the transmitter cell, whereas a stochastic approach was used for the receiver cell. Two previously hypothesized mechanisms for the AI-2 pathway of the receiver cell were modeled and compared.The transmitter cell has an inducible promoter that controls AI-2 synthesis under different sugar inputs. Light is produced once AI-2 binds to the membrane receptors of the receiver cell, V. harveyi. The functionality of the circuit was determined by the gene expression of the transmitter cell and the luminescence level of the receiver cell when exposed to the supernatants of the transmitter cell. A five-fold signal increase was observed when the receiver cell was grown in the transmitter cell supernatant containing AI-2.
Notes: Source: Masters Abstracts International, Volume: 44-06, page: 2879.

Thesis (M.A.Sc.)--University of Toronto, 2006.

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