Receptor signal transduction protocols
By: edited by Gary B. Willars, R.A. John Challiss
Contributors: Willars, Gary B; Challiss, R. A. J.
Edition: 2nd ed
Series: Methods in molecular biology -- 259; Methods in molecular biology (Clifton, N.J.) --  259
Publisher: Humana Press
Published: 2004
Place of publication: Totowa, N.J
Language: eng
Number of pages: 418
Subjects: Cell receptors -- Research -- Methodology; Cellular signal transduction -- Research -- Methodology
Description: A collection of state-of-the-art methods for interrogating ligand-receptor and receptor-effector interactions. Methods emphasize the regulation of G protein-coupled receptors and focus on receptor characterization, methods for investigating how receptors couple to G proteins, and approaches to studying how receptor expression and function is regulated. The easily reproducible protocols include chimeric receptor construction, antibody generation, transient and stable transfection methods, hybridization, as well as immunocytochemistry, functional assays of G protein function, and antisense methods for defining receptor-effector coupling pathways.
Notes: Includes bibliographical references and index