Sortase-mediated labeling of M13 bacteriophage and the formation of multi-phage structures
Contributors: Belcher, Angela M.; Hu, Evelyn L.; Shih, William M.; Ploegh, Hidde L.; Hogle, James M.; Springer, Timothy A.
Published: 2012
Language: eng
Description: M13 filamentous bacteriophage has been used as a biotemplate for the nucleation of materials. Phage is an ideal and diverse scaffold with its large aspect ratio and ability to display biomolecules to bind a range of targets. To form more complex patterned materials, interactions between the phage must be specific and reliable. We develop a phage labeling method using sortase enzymes to create multi-phage nanostructures.
Notes: Keywords: M13 bacteriophage, nanostructures, sortase.

Thesis Ph.D. Harvard University 2012