Chloroplast research in arabidopsis
Subtitle: methods and protocols
By: edited by R. Paul Jarvis
Series: Methods in molecular biology -- 774; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 774.; Springer protocols
Publisher: Humana Press
Published: 2011
Place of publication: New York
Language: eng
Number of pages: 374
ISBN-13: 9781617792366; 9781617792335
Subjects: Methodology; Chloroplasts; Arabidopsis; Research
Notes: Includes bibliographical references and index.
Table of contents: title: Screening or selection for chloroplast biogenesis mutants of Arabidopsis, following chemical or insertional mutagenesis  -- Enrique Lopez-Juez and Alison Hills; title: Analysis of plastid number, size, and distribution in Arabidopsis plants by light and fluorescence microscopy  -- Kevin Pyke; title: Immunofluorescence microscopy for localization of Arabidopsis chloroplast proteins  -- Stanislav Vitha and Katherine W. Osteryoung; title: Transient expression and analysis of chloroplast proteins in Arabidopsis protoplasts  -- Dong Wook Lee and Inhwan Hwang; title: Visualisation of stromules on Arabidopsis plastids  -- John C. Gray, James A. Sullivan, and Christine A. Newell; title: Analysis of chloroplast movement and relocation in Arabidopsis  -- Masamitsu Wada and Sam-Geun Kong; title: Studying starch content and sedimentation of amyloplast statoliths in Arabidopsis roots  -- John Stanga, Allison Strohm, and Patrick H. Masson; title: Studying Arabidopsis chloroplast structural organisation using transmission electron microscopy  -- Stefan Hyman and R. Paul Jarvis; title: Transplastomics in Arabidopsis : progress toward developing an efficient method  -- Kerry Ann Lutz, Arun Azhagiri, and Pal Maliga; title: Isolation, quantification, and analysis of chloroplast DNA  -- Beth A. Rowan and Arnold J. Bendich; title: Measurement of transcription rates in Arabidopsis chloroplasts  -- Yan O. Zubo, Thomas Borner, and Karsten Liere; title: Studying the structure and processing of chloroplast transcripts  -- Alice Barkan; title: In vitro RNA-binding assay for studying trans-factors for RNA editing in chloroplasts  -- Toshiharu Shikanai and Kenji Okuda; title: Studying translation in Arabidopsis chloroplasts  -- Paolo Pesaresi; title: Studying proteases and protein turnover in Arabidopsis chloroplasts  -- Lars L.E. Sjogren and Adrian K. Clarke; title: In silico methods for identifying organellar and suborganellar targeting peptides in Arabidopsis chloroplast proteins and for predicting the topology of membrane proteins  -- Sandra K. Tanz and Ian Small; title: Rapid isolation of Arabidopsis chloroplasts and their use for in vitro protein import assays  -- Henrik Aronsson and R. Paul Jarvis; title: Energetic manipulation of chloroplast protein import and the use of chemical cross-linkers to map protein-protein interactions  -- Hitoshi Inoue ... [et al.]; title: Isolation of Arabidopsis thylakoid membranes and their use for in vitro protein insertion or transport assays  -- Thomas Bals and Danja Schunemann; title: Determining the location of an Arabidopsis chloroplast protein using in vitro import followed by fractionation and alkaline extraction  -- Chiung-Chih Chu and Hsou-min Li; title: Studying Arabidopsis envelope protein localization and topology using thermolysin and trypsin proteases -- John Froehlich.