The regulation of glucagon-like peptide-2 receptor signaling and cell surface expression.
Published: 2005
Language: eng
Number of pages: 218
Description: Glucagon-like peptide-2 (GLP-2) is a peptide hormone released from a subset of endocrine cells within the gastrointestinal tract and neurons of the central nervous system. GLP-2 elicits its cytoprotective and proliferative effects through activation of its cognate receptor, a member of the Family B subgroup of G protein coupled receptors (GPCR). The mechanisms controlling GPCR desensitization, endocytosis, and trafficking have been largely elucidated using receptor models from the Family A Rhodopsin-like GPCRs. Little is known about the mechanisms regulating signaling and expression of the structurally distinct receptors for glucagon and the glucagon-like peptides. Using an  in vitro cell model, we investigated the effects of acute GLP-2 receptor (GLP-2R) activation on cell surface receptor expression and down-stream signaling events. A combination of cell-based assays and site-directed mutagenesis identified receptor interacting proteins and revealed mechanisms regulating GLP-2 receptor desensitization, endocytosis, and intracellular trafficking.As long-acting analogs of GLP-2 are currently in clinical trials for the treatment of gastrointestinal disease, the potential consequences of persistent GLP-2R signaling are of significant clinical relevance. Given the diversity of physiological actions regulated by the Family B GPCRs, delineation of the mechanisms regulating their signaling may facilitate understanding of how peptide hormone action is tightly regulated.We show that the GLP-2R undergoes rapid and persistent agonist-induced desensitization of its cAMP response. Furthermore, the GLP-2R internalizes in a lipid-raft-dependent and dynamin-independent manner, but is quickly trafficked into the canonical endosomal-recycling pathway. We demonstrate that serine residues within the distal GLP-2R C-terminus facilitate a stable interaction of beta-arrestin-2 with the receptor. However, neither the C-terminal domain nor the stable beta-arrestin-2/GLP-2R association are needed to mediate G protein-dependent effector coupling, homologous desensitization, or internalization. In contrast, the GLP-2R C-terminus is necessary for PKA-dependent heterologous desensitization of receptor signaling, while PKC activity failed to modulate GLP-2R signaling in vitro. To further investigate the potential consequences of down-regulated GLP-2R signaling, we conducted a series of studies to identify potential GLP-2R antagonists. We show that the N-terminally truncated GLP-2 analogs, GLP-2 (3-33) and GLP-2 (5-33), competitively inhibited G protein-dependent signaling of the GLP-2R.
Notes: Source: Dissertation Abstracts International, Volume: 66-10, Section: B, page: 5233.

Thesis (Ph.D.)--University of Toronto, 2005.

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