Ras signaling
Subtitle: methods and protocols
By: edited by Lorenza Trabalzini, Department of Biotechnology, Chemistry, and Pharmacy, University of Siena, Siena, Italy, Saverio Francesco Retta, Department of Clinical and Biological Sciences, University of Torino, Torino, Italy
Contributors: Springer Science+Business Media
Series: Methods in molecular biology -- 1120; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 1120,; Springer protocols (Series)
Publisher: Humana Press
Published: 2014
Place of publication: New York
Language: eng
Number of pages: 425
ISBN-13: 9781627037907; 9781627037914
Copyright date: 2014
Subjects: Ras oncogenes; Laboratory manuals; Ras proteins; Cellular signal transduction; Guanosine triphosphatase; Guanosine triphosphatase genes; GTPase-activating protein; Cell transformation; Ras Proteins; Laboratory Manuals; Signal Transduction; Ras; Signaltransduktion
Notes: Includes bibliographical references and index.
Table of contents: title: The Ras superfamily of small GTPases : the unlocked secrets  -- Luca Goitre [and three others]; title: A novel method for the production of fully modified K-Ras 4B  -- Tanmay S. Chavan [and four others]; title: Metabolic labeling of Ras with tritiated palmitate to monitor palmitoylation and depalmitoylation  -- Frederick D. Tsai [and three others]; title: Ubiquitination of Rac1 by inhibitors of apoptosis (IAPs)   -- Tripat Kaur Oberoi-Khanuja and Krishnaraj Rajalingam; title: Ras GTPases are both regulators and effectors of redox agents  -- Elisa Ferro [and four others]; title: Biophysical and proteomic characterization strategies for cysteine modifications in Ras GTPases  -- G. Aaron Hobbs, Harsha P. Gunawardena, and Sharon L. Campbell; title: Use of the yeast two-hybrid technology to isolate molecular interactions of Ras GTPases  -- Elisa Ferro, Eva Baldini, and Lorenza Trabalzini; title: Screening for MAPK modulators using an In-Cell Western assay  -- Simon Schnaiter [and seven others]; title: Behavioral methods for the study of the Ras-ERK pathway in memory formation and consolidation : passive avoidance and novel object recognition tests  -- Raffaele d'Isa, Riccardo Brambilla, and Stefania Fasano; title: Functional phosphoproteomics of oncogenic KRAS signaling  -- Putty-Reddy Sudhir and Jeou-Yuan Chen; title: Pull-down assay for analysis of integrin-mediated activation of Rap proteins in adherent platelets  -- Gianni Francesco Guidetti and Mauro Torti; title: Combined pulldown and time-lapse microscopy studies for determining the role of Rap1 in the crosstalk between  integrins and cadherins   -- Luca Goitre and Saverio Francesco Retta; title: Fluorescence microscopy study of Rap1 subcellular localization  -- Luca Goitre, Valentina Cutano, and Saverio Francesco Retta; title: An in vitro system to evaluate the scaffold function of the RalA effector protein RalBP1  -- David F. Kashatus; title: Analysis of the Rit subfamily GTPase-mediated signaling and neuronal differentiation and survival  -- Geng-Xian Shi and Catherine N. Kaminski; title: Immunofluorescence methods in studies of the GTPase Ran and its effectors in interphase and in mitotic cells  -- Giulia Guarguaglini, Valeria de Turris, and Patrizia Lavia; title: High-resolution scanning electron microscopy for the imaging of nuclear pore complexes and Ran-mediated transport  -- Lihi Shaulov, Boris Fichtman, and Amnon Harel; title: Effector recruitment method to study spatially regulated activation of Ras and Rho GTPases  -- Lauren P. Huff, Molly J. DeCristo, and Adrienne D. Cox; title: Real-time visualization and quantification of native Ras activation in single living cells  -- Christoph Biskup and Ignacio Rubio; title: Nanoclustering and heterogeneous membrane diffusion of Ras studied by FRAP and RICS analysis  -- Camilo Guzmán, Maja Šolman, and Daniel Abankwa; title: Analyzing the roles of Rho GTPases in cancer cell migration with a live cell imaging 3D-morphology-based assay  -- Audrey Colomba and Anne J. Ridley; title: Analysis of Rho GTPase-induced localization of nanoscale adhesions using fluorescence nanoscopy  -- Annica K.B. Gad [and three others]; title: Yeast as a model for Ras signalling  -- Renata Tisi, Fiorella Belotti, and Enzo Martegani; title: Methods to study the Ras2 protein activation state and the subcellular localization of Ras-GTP in Saccharomyces cerevisiae  -- Sonia Colombo and Enzo Martegani; title: Ras proteins signaling in the early metazoan Dictyostelium discoideum -- Enrico Bracco and Barbara Pergolizzi.