DNA sequencing III
Subtitle: dealing with difficult templates
By: edited by Jan Kieleczawa.
Contributors: Kieleczawa, Jan.
Publisher: Jones and Bartlett Publishers
Published: 2009
Place of publication: Sudbury, Mass
Language: eng
Number of pages: 184
ISBN-13: 9780763742973
Subjects: DNA -- Analysis; Nucleotide sequence -- Methodology; Sequence Analysis, DNA -- methods; Base Sequence; Templates, Genetic
Other titles: DNA sequencing three; DNA sequencing 3
Notes: Includes bibliographical references and index.
Table of contents: title: Controlled heat-denaturation of DNA plasmids and PCR fragments; title: Heat denaturation is an effective step in sequencing of many difficult DNA templates; title: Solutions for sequencing difficult regions; title: Improving sequence results from difficult templates with Phi 29 DNA polymerase and nucleotide analogs: the TempliPhi sequence resolver kit; title: Sequencing through various secondary structures: detailed studies of pDEST vectors and other DNA templates with hairpins; title: Transcriptional sequencing as a tool for reading difficult-to-read templates; title: Bias-free cloning of "unclonable" DNA for simplified genomic finishing; title: Bioinformatics tools to aide sequencing of difficult templates.