characterization of a novel family C G-protein coupled receptor musGPRC6A.
Published: 2005
Language: eng
Number of pages: 71
Description: The mouse GPRC6A receptor is a Family C orphan G-protein coupled receptor that shares amino acid sequence identity with the fish 5.24 odorant receptor, the mammalian calcium-sensing receptor and the metabotropic glutamate receptors. Using anti-peptide polyclonal antibodies generated against musGPRC6A, I determined that mouse heart expressed musGPRC6A at the highest apparent level, while lower levels were present day-17 mouse embryos and possibly in tongue. Immunocytochemical analyses of transfected human embryonic kidney cells expressing musGPRC6A showed that the receptor is expressed both on the cell surface and on the endoplasmic reticulum and nuclear envelope. Biochemical studies demonstrated that musGPRC6A is glycosylated at asparagine residues and forms dimers through disulfide linkages. The tissue and subcellular distributions of musGPRC6A have suggested a possible physiological role in regulating heart function, while the ligand activation pattern could be indicative of a role in taste-sensing.
Notes: Source: Masters Abstracts International, Volume: 44-01, page: 0327.

Thesis (M.Sc.)--University of Toronto, 2005.

Electronic version licensed for access by U. of T. users.

ROBARTS MICROTEXT copy on microfiche.