Claudins
Subtitle: methods and protocols
By: edited by Kursad Turksen
Series: Methods in molecular biology -- v. 762; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 762.; Springer protocols
Publisher: Humana; Springer
Published: 2011
Place of publication: New York
Language: eng
Number of pages: 447
ISBN-13: 9781617791840; 9781617791857
Subjects: Membrane proteins; Tight junctions (Cell biology); Claudins; Tight Junctions
Description: [Publisher-supplied data] Since the initial discovery of the claudins, the field of tight junctions and cell polarity has become increasingly energized, mainly due to the fact that the claudins have provided an opportunity to understand the molecular basis of the tight junction function and their tissue specific roles. In Claudins: Methods and Protocols, experts and pioneers in the field provide an extensive collection of techniques involving these tight junction proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Claudins: Methods and Protocols serves as an ideal guide for all scientists seeking to further our understanding of this vital area of research.
Notes: Includes bibliographical references and index.
Table of contents: title: Measuring size-dependent permeability of the tight junction using PEG profiling  -- Christina M. Van Itallie and James M. Anderson; title: Biochemical analysis of claudin-binding compatibility  -- Christina Ward and Michael Koval; title: Electrophysiological Characterization of Claudin Ion Permeability Using Stably Transfected Epithelial Cell Lines  -- Alan S.L. Yu; title: Tight junction, intercellular seal as a cell signaling player : protocols for examination of its status  -- Makoto Osanai; title: Interactions between Clostridium perfringens enterotoxin and claudins  -- Susan L. Robertson and Bruce A. McClane; title: Biophysical methods to probe claudin-mediated adhesion at the cellular and molecular level  -- Sri Ram Krishna Vedula ... [et al.]; title: Detection of tight junction barrier function in vivo by biotin  -- Lei Ding ... [et al.]; title: Coculture method to examine interactions between claudin isoforms in tight junction-free HEK293 cells and tight junction-bearing MDCK II cells  -- Tetsuichiro Inai; title: Claudin-4 : functional studies beyond the tight junction  -- Holly A. Eckelhoefer ... [et al.]; title: Methods to analyze subcellular localization  and intracellular trafficking of claudin-1  -- P. Jaya Kausalya and Walter Hunziker; title: Claudin family proteins in Caenorhabditis elegans  -- Jeffrey S. Simske and Jeff Hardin; title: In vivo imaging of tight junctions using claudin-EGFP transgenic medaka  -- Tatsuo Miyamoto, Mikio Furuse, and Makoto Furutani-Seiki; title: Claudins in a primary cultured puffer fish (Tetraodon nigroviridis) gill epithelium  -- Phuong Bui and Scott P. Kelly; title: Manipulating claudin expression in avian embryos  -- Michelle M. Collins and Aimee K. Ryan; title: Identification of claudins by western blot and immunofluorescence in different cell lines and tissues  -- Lorenza Gonzalez-Mariscal, Erika Garay, and Miguel Quiros; title: Expression and function of claudins in hepatocytes  -- Takashi Kojima and Norimasa Sawada; title: Analysis of changes in the expression pattern of claudins using salivary acinar cells in primary culture  -- Junko Fujita-Yoshigaki; title: Development of biological tools to study claudins in the male reproductive tract  -- Daniel G. Cyr ... [et al.]; title: Using molecular tracers to assess the integrity of the intestinal epithelial barrier in vivo  -- Julian A. Guttman; title: Laboratory methods in the study of endometrial claudin-4  -- Paulo Serafini ... [et al.]; title: Role of claudins in oxidant-induced alveolar epithelial barrier dysfunction  -- Yu Sun, Richard D. Minshall, and Guochang Hu; title: Tracing the endocytosis of claudin-5 in brain endothelial cells  -- Svetlana M. Stamatovic, Richard F. Keep, and Anuska V. Andjelkovic; title: Quantitative in situ analysis of claudin expression at the blood-retinal barrier  -- Heping Xu and Janet Liversidge; title: MMP-mediated disruption of claudin-5 in the blood-brain barrier of rat brain after cerebral ischemia  -- Yi Yang and Gary A. Rosenberg; title: Claudin-5 expression in in vitro models of the blood-brain barrier  -- Itzik Cooper, Katayun Cohen-Kashi-Malina, and Vivian I. Teichberg; title: HIV-1-induced alterations of claudin-5 expression at the blood-brain barrier level  -- Ibolya E. Andras and Michal Toborek; title: Enhanced immunohistochemical resolution of claudin proteins in glycolmethacrylate-embedded tissue biopsies  -- Jane E. Collins ... [et al.]; title: Claudin-16/paracellin-1, cloning, expression, and its role in tight junction functions in cancer and endothelial Cells  -- Tracey A. Martin and Wen G. Jiang; title: Dynamics of claudins expression in colitis and colitis-associated cancer in rat  -- Yoshiaki Arimura, Kanna Nagaishi, and Masayo Hosokawa; title: Anti-claudin-4-conjugated highly luminescent nanoparticles as biological labels for pancreatic cancer sensing -- Ken-Tye Yong.