PCR primer design
By: edited by Chhandak Basu, Department of Biology, California State University, Northridge, Los Angeles, CA, USA
Edition: Second edition.
Series: Methods in molecular biology -- 1275; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- 1275,; Springer protocols (Series)
Publisher: Humana Press; Humana
Published: 2015
Place of publication: New York
Language: eng
Number of pages: 216
ISBN-13: 9781493923649; 9781493923656
Copyright date: 2015
Subjects: Polymerase chain reaction; Gene amplification
Description: "This volume provides an overview on design PCR primers for successful DNA amplification. Chapters focus on primer design strategies for quantitative PCR, in silico PCR primer design , and primer design using software. Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls." --
Notes: Includes bibliographical references and index.
Table of contents: title: Fast masking of repeated primer binding sites in eukaryotic genomes / Reidar Andreson, Lauris Kaplinksi, and Maido Remm; title: Primer design for PCR reactions in forensic biology / Kelly M. Elkins; title: Design of primers and probes for quantitative real-time PCR methods / Alicia Rodríguez, Mar Rodríguez, Juan J. Córdoba, and María J. Andrade; title: Large-scale neucleotide sequence alignment and sequence variability assessment to identify the evolutionarily highly conserved regions for universal screening PCR assay design: an example of influenza A virus / Alexander Nagy, Tomáš Jiřinec, Lenka Černíková, Helena Jiřincová, and Martina Havlíčková; title: Low-concentration initiator primers improve the amplification of gene targets with high sequence variability / Kenneth E. Pierce and Lawrence J. Wangh; title: Multiplex PCR primer design for simultaneousu detection of multiple pathogens / Wenchao Yan; title: Degenerate primer design for highly variable genomes / Kelvin Li, Susmita Shrivastava, and Timothy B. Stockwell; title: Allele-specific real-time polymerase chain reaction as a tool for urate transporter 1 mutation detection / Juliet O. Makanga, Antonius Christianto, and Tetsuya Inazu; title: MultiPLX: automatic grouping and evaluation of PCR primers / Lauris Kaplinski and Maido Remm; title: In silico PCR primer designing and validation / Anil Kumar and Nikita Chrodia; title: Primer design using Primer Express® for SYBR green-based quantitative PCR / Amarjeet Singh and Girdhar K. Pandey; title: Designing primers for SNaPshot technique / Greciane Gaburro Paneto and Francisco de Paula Careta; title: Rapid and simple method of qPCR primer design / Brenda Thornton and Chhandak Basu; title: PRIMEGENSw3: a web-based tool for high-throughput primer and probe design / Garima Kushwaha, Gyan Prakash Srivastava, and Dong Xu; title: Selecting specific PCR primers with MFEprimer / Wubin Qu and Chenggang Zhang.