Prooxidant activity and cellular effects of indole-3-acetic acid derivative radicals.
Published: 2004
Language: eng
Number of pages: 51
Description: The prooxidant activity of radicals formed when IAA and its derivatives were metabolically oxidized by peroxidase/H2O2, were compared. It was found that the effectiveness of IAA for catalyzing the cooxidation of ascorbate, NADH or GSH increased as their one electron redox potential decreased. IAA and its derivatives cooxidized NADH, ascorbate, GSH and hepatocyte GSH with an order of effectiveness of 5MeO-2Me-IAA > 2Me-IAA > 5MeO-IAA > IAA. The rate of NADH or ascorbate cooxidation was faster at pH 7.4 than pH 6.0, whereas GSH cooxidation was faster at pH 6.0. The order of efficiency of IAA and its derivatives in catalyzing lipid peroxidation correlated with their rate of oxidation by HRP-I and their one-electron potential. Hepatocytes incubated with IAA derivatives and peroxidase/H2O2 resulted in cytotoxicity, GSH oxidation, lipid peroxidation and "ROS" formation, prooxidant activities can therefore be attributed to radicals rather than the end product methylene-2-oxindole.
Notes: Adviser: Peter J. O'Brien.

Thesis (M.Sc.)--University of Toronto, 2004.

Electronic version licensed for access by U. of T. users.

Source: Masters Abstracts International, Volume: 42-06, page: 2196.

MICR copy on microfiche (1 microfiche).