Gene knockout protocols
Contributors: Wurst, Wolfgang, 1955-
Edition: 2nd ed. / edited by Ralf Kühn, Wolfgang Wurst.
Series: Methods in molecular biology -- 530; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 530.; Springer protocols
Publisher: Humana Press
Published: 2009
Place of publication: New York, NY
Language: eng
Number of pages: 516
ISBN-13: 9781934115268; 9781597454711
Notes: Includes bibliographical references and index.
Table of contents: title: Overview on mouse mutagenesis / Ralf Kühn and Wolfgang Wurst; title: Construction of gene-targeting vectors by recombineering / Song-Choon Lee, Wei Wang, and Pentao Liu; title: Gene-trap vectors and mutagenesis / Silke De-Zolt ... [et al.]; title: Chromosome engineering in ES cells / Louise vander Weyden, Charles Shaw-Smith, and Allan Bradley; title: Gene modification in embryonic stem cells by single-stranded DNA oligonucleotides / Marieke Aarts ... [et al.]; title: Generation of shRNA transgenic mice / Christiane Hitz ... [et al.]; title: Mutagenesis of mouse embryonic stem cells with ethylmethanesulfonate / Robert Munroe and John Schimenti; title: Gene targeting in mouse embryonic stem cells / Lino Tessarollo ... [et al.]; title: Manipulating mouse embryonic stem cells / Eileen Southon and Lino Tessarollo; title: ES cell line establishment / Heidrun Kern and Branko Zevnik; title: Generation of double-knockout embryonic stem cells / Eva Wielders, Marleen Dekker, and Hein te Riele; title: Differentiation analysis of pluripotent mouse embryonic stem (ES) cells in vitro / Insa S. Schroeder ... [et al.]; title: Cloning of ES cells and mice by nuclear transfer / Sayaka Wakayama, Satoshi Kishigami, and Teruhiko Wakayama; title: Isolation, microinjection, and transfer of mouse blastocysts / Susan W. Reid and Lino Tessarollo; title: Aggregation chimeras : combining ES cells, diploid, and tetraploid embryos / Mika Tanaka ... [et al.]; title: VelociMouse : fully ES cell-derived F0-generation mice obtained from the injection of ES cells into eight-cell-stage embryos / Thomas M. DeChiara ... [et al.]; title: Generation of Cre recombinase-expressing transgenic mice using bacterial artificial chromosomes / Jan Rodriguez Parkitna, David Engblom, and Günther Schütz; title: Inducible Cre mice / Susanne Feil, Nadejda Valtcheva, and Robert Feil; title: Creation and use of a Cre recombinase transgenic database / Andras Nagy, Lynn Mar, and Graham Watts; title: Transposon mutagenesis in mice / David A. Largaespada; title: Lentiviral transgenesis / Alexander Pfeifer and Andreas Hofmann; title: Sperm cryopreservation and in vitro fertilization / Susan Marschall, Auke Boersma, and Martin Hrabé de Angelis; title: Influence of genetic background on genetically engineered mouse phenotypes / Thomas Doetschman; title: Pathologic phenotyping of mutant mice / Roderick T. Bronson; title: Systemic first-line phenotyping / Valérie Gailus-Durner ... [et al.]