Sirtuins
Subtitle: methods and protocols
By: edited by Matthew D. Hirschey, Institute of Molecular Physiology, Duke University Medical Center, Durham, NC, USA
Contributors: Springer Science+Business Media
Series: Methods in Molecular Biology -- 1077; Springer Protocols; Methods in molecular biology (Clifton, N.J.) -- v. 1077.; Springer protocols (Series)
Publisher: Humana Press
Published: 2013
Place of publication: New York
Language: eng
Number of pages: 314
ISBN-13: 9781627036368; 9781627036375
Copyright date: 2013
Subjects: Proteins; Sirtuins
Notes: Includes bibliographical references and index.
Table of contents: title: Introduction: sirtuins in aging and diseases  -- Leonard Guarente; title: Sirtuins in yeast: phenotypes and tools  -- Scott Tsuchiyama [and four others]; title: C. elegans sirtuins  -- Mohan Viswanathan and Heidi A. Tissenbaum; title: Genetic and biochemical tools for investigating sirtuin function in Drosophila melanogaster  -- Jason G. Wood, Rachel Whitaker, and Stephen L. Helfand; title: Generating mammalian sirtuin tools for protein-interaction analysis  -- Kathleen A. Hershberger [and three others]; title: Mass spectrometry-based detection of protein acetylation  -- Yu Li [and three others]; title: SILAC-based quantification of Sirt1-responsive lysine acetylome  -- Yue Chen, Gozde Colak, and Yingming Zhao; title: Targeted quantitation of acetylated lysine peptides by selected reaction monitoring mass spectrometry  -- Matthew J. Rardin, Jason M. Held, and Bradford W. Gibson; title: Identification of deacetylase substrates with the biotin switch approach  -- J. Will Thompson, Alex Robeson, and Joshua L. Andersen; title: Assaying chromatin sirtuins  -- Lei Zhong [and four others]; title: Measurement of sirtuin enzyme activity using a substrate-agnostic fluorometric nicotinamide assay  -- Basil P. Hubbard and David A. Sinclair; title: Detecting sirtuin-catalyzed deacylation reactions using 32P-labeled NAD and thin-layer chromatography  -- Anita Zhu, Xiaoyang Su, and Hening Lin; title: Chemical acetylation and deacetylation  -- Kristofer S. Fritz; title: Accurate measurement of nicotinamide adenine dinucleotide (NAD+) with high-performance liquid chromatography  -- Jun Yoshino and Shin-ichiro Imai; title: In vivo measurement of the acetylation state of sirtuin substrates as a proxy for sirtuin activity  -- John Dominy, Pere Puigserver, and Carles Cantó; title: Oxygen flux analysis to understand the biological function of sirtuins  -- Dongning Wang [and three others]; title: The emerging links between sirtuins and autophagy  -- In Hye Lee, Jeanho Yun, and Toren Finkel; title: Methods to study the role of sirtuins in genome stability  -- Paloma Martínez-Redondo and Alejandro Vaquero; title: Circadian measurements of sirtuin biology  -- Kathryn Moynihan Ramsey [and five others]; title: Utilizing calorie restriction to evaluate the role of sirtuins in healthspan and lifespan of mice -- Jessica Curtis and Rafael de Cabo.