Genomic location analysis of Myc and Mnt.
Published: 2007
Language: eng
Number of pages: 120
ISBN-13: 9780494274828
Description: Myc is an oncogenic transcription factor that controls diverse cellular functions, through the activation and repression of target genes. Mxd family members have been shown to repress transcription at Myc activated genes. The current dogma is that this antagonistic relationship between Myc and Mxd is the key to the regulation of Myc function. However, knockout studies have shown that one Mxd family member, Mnt, may be more important in inhibiting transformation than other family members.This data has led to our hypothesis that the identification of Mnt target genes will help us uncover the subset of Myc target genes that are important for transformation. We have identified 481 genomic loci bound by Myc and 328 genomic loci bound by Mnt by combining chromatin immunoprecipitation (ChIP) with promoter enriched microarrays (chip) in HL60 cells. The ChIP has been optimized to examine Myc and Mnt binding in a model of Myc-dependent transformation.
Notes: Source: Masters Abstracts International, Volume: 45-06, page: 3060.

Thesis (M.Sc.)--University of Toronto, 2007.

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