Plant endosomes
Subtitle: methods and protocols
By: edited by Marisa S. Otegui, Departments of Botany and Genetics, University of Wisconsin-Madison, Madison, WI, USA
Series: Methods in molecular biology -- 1209; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 1209.; Springer protocols (Series)
Publisher: Humana Press
Published: 2014
Place of publication: New York
Language: eng
Number of pages: 286
ISBN-13: 9781493914197; 9781493914203
Copyright date: 2014
Subjects: Endosomes; Laboratory Manuals; Laboratory manuals; Plant organelles; Plant Proteins
Description: "This book contains a collection of protocols and techniques to analyze in vivo trafficking of endocytic/endosomal cargo, including lipids, fluids, proteins, and ligands, ultrastructural features of endosomes by high-pressure freezing/freeze-substitution and electron tomography, protein-protein interactions in the endosomal and endomembrane system, sorting defects in the transport of vacuolar storage proteins, function conservation of plant endosomal proteins, endosomal traffi cking during plant responses to pathogens, protein composition of endosomes and endocytic vesicles, ubiquitination of endosomal cargo proteins, and identifi cation of novel endosomal components by chemical genomics and proteomics."--
Notes: Includes bibliographical references and index.
Table of contents: title: Use of FM dyes to analyze plant endocytosis  -- Kateřina Malínská, Adriana Jelínková, and Jan Petrášek; title: Sterol dynamics during endocytic trafficking in Arabidopsis  -- Thomas Stanislas, Markus Grebe, and Yohann Boutté; title: Live microscopy analysis of endosomes and vesicles in tip-growing root hairs  -- Miroslav Ovečka, Irene Lichtscheidl, and Jozef Šamaj; title: Analysis of fluid-phase endocytosis in (intact) plant cells  -- Vera Bandmann, Peter Haub, and Tobias Meckel; title: Immunogold labeling and electron tomography of plant endosomes  -- Alexandra Chanoca and Marisa S. Otegui; title: Investigating protein-protein interactions in the plant endomembrane system using multiphoton-induced FRET-FLIM  -- Jennifer Schoberer and Stanley W. Botchway; title: Analysis of Rab GTPase-effector interactions by bimolecular fluorescence complementation  -- Emi Ito and Takashi Ueda; title: In vivo imaging of brassinosteroid endocytosis in Arabidopsis  -- Niloufer G. Irani, Simone Di Rubbo, and Eugenia Russinova; title: Analysis of prevacuolar compartment-mediated vacuolar proteins transport  -- Caiji Gao, Yi Cai, Xiaohong Zhuang, and Liwen Jiang; title: Evaluation of defective endosomal trafficking to the vacuole by monitoring seed storage proteins in Arabidopsis thaliana  -- Tomoo Shimada, Yasuko Koumoto, and Ikuko Hara-Nishimura; title: Trans-species complementation analysis to study function conservation of plant endosomal sorting complex required for transport (ESCRT) proteins  -- Francisca C. Reyes; title: Re-elicitation assay to correlate flg22-signaling competency with ligand-induced endocytic degradation of the FLS2 receptor  -- Michelle E. Leslie and Antje Heese; title: Preparation of enriched plant clathrin-coated vesicles by differential and density gradient centrifugation  -- Gregory D. Reynolds, Ben August, and Sebastian Y. Bednarek; title: Proteomics of endosomal compartments from plants case study : isolation of trans-golgi network vesicles  -- Eunsook Park and Georgia Drakakaki; title: Analysis of global ubiquitylation and ubiquitin-binding domains involved in endosomal trafficking  -- Kamila Kalinowska and Erika Isono; title: Analysis of endocytosis and ubiquitination of the BOR1 transporter  -- Koji Kasai, Junpei Takano, and Toru Fujiwara; title: Ubiquitination of plant immune receptors  -- Jinggeng Zhou, Ping He, and Libo Shan; title: Ubiquitylation-mediated control of polar auxin transport : analysis of Arabidopsis PIN2 auxin transport protein  -- Johannes Leitner and Christian Luschnig; title: Chemical genomics screening for biomodulators of endomembrane system trafficking  -- Carlos Rubilar-Hernández, Glenn R. Hicks, and Lorena Norambuena; title: Integrative chemical proteomics and cell biology methods to study endocytosis and vesicular trafficking in Arabidopsis -- Tomáš Takáč, Tibor Pechan, Olga Šamajová, and Jozef Šamaj.