Peptide nucleic acids
Subtitle: methods and protocols
By: edited by Peter E. Nielsen, Department of Cellular and Molecular Medicine, University of Copenhagen, Copenhagen, Denmark, Daniel H. Appella, Section on Synthetic Bioactive Molecules, Laboratory of Bioorganic Chemistry, NIDDK, NIH, DHHS, Bethesda, MD, USA .
Contributors: Springer Science+Business Media
Edition: Second edition.
Series: Methods in molecular biology -- 1050; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 1050,; Springer protocols (Series)
Publisher: Humana Press
Published: 2014
Place of publication: New York
Language: eng
Number of pages: 239
ISBN-13: 9781627035521; 9781627035538
Copyright date: 2014
Subjects: Peptides; Nucleic acids; Peptide Nucleic Acids; Laboratory Manuals; ADN; Dérivés; Nucleïnezuren; Peptiden; Wetenschappelijke technieken; Labortechnik; Peptid-Nucleinsäuren
Description: Exemplifying and illustrating recent exciting advances in PNA chemistry, the second edition of Peptide Nucleic Acids: Methods and Protocols serves as a vital complement to the first edition of the book. Since the discovery of peptide nucleic acids, many interesting new derivatives and analogues in terms of nucleic acid recognition specificity and affinity have emerged. Also, as this detailed volume presents, great ingenuity in exploiting the unique properties of PNAs for a wide variety of applications within drug discovery, medical diagnostics, chemical biology and nanotechnology has unfolded. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Peptide Nucleic Acids: Methods and Protocols, Second Edition serves as a source of useful specific methods and protocols as well as a source of inspiration for future developments.
Notes: Includes bibliographical references and index.
Table of contents: title: 1. MiniPEG-[gamma]PNA / Arunava Manna, Srinivas Rapireddy, Raman Babal and Danith H. Ly; title: 2. Cyclopentane peptide nucleic acids / Ethan A. Englund, Ning Zhang, and Daniel H. Appella; title: 3. Chiral PNAs with constrained open-chain backbones / Roberto Corradini, Tullia Tedeschi, Stefano Sforza and Rosangela Marchelli; title: 4. Synthesis, characterization, and evaluation of radiometal-containing peptide nucleic acids / Holger Stephan, Christian Foerster, and Gilles Gasser; title: 5. Preparation of metal-containing peptide nucleic acid bioconjugates on the solid phase / Gilles Gasser; title: 6. Formation and characterization of PNA-containing heteroquadruplexes / Bruce A. Armitage; title: 7. Sequence selective recognition of double-stranded RNA using triple helix-forming peptide nucleic acids / Thomas Zengeya, Pankaj Gupta, and Eriks Rozners; title: 8. Assembly of PNA-tagged small molecules, peptides, and carbohydrates onto DNA templates : programming the combinatorial pairing and inter-ligand distance / Nicolas Winssinger, Katarzyna Gorska, Mihai Ciobanu, Jean Pierre Daguer and Sofia Barluenga; title: 9. Site-selective scission of human genome using PNA-based artificial restriction DNA cutter / Kenichiro Ito and Makoto Komiyama; title: 10. PNA openers and their applications for bacterial DNA diagnostics / Irina V. Smolina and Maxim D. Frank-Kamenetskii; title: 11. DNA-templated native chemical ligation of functionalized peptide nucleic acids : a versatile tool for single base-specific detection of nucleic acids / Alexander Roloff, Simon Ficht, Christian Dose and Oliver Seitz; title: 12. Use of peptide nucleic acids (PNAs) for genotyping by solution and surface methods / Stefano Sforza, Tullia Tedeschi, Mariangela Bencivenni, Alessandro Tonelli, Roberto Corradini and Rosangela Marchelli; title: 13. Analysis of PNA hybridization by surface plasmon resonance / Bruce A. Armitage; title: 14. PNA fluorescent in situ hybridization (FISH) for rapid microbiology and cytogenetic analysis / Henrik Stender, Brett Williams, and James Coull; title: 15. Rapid miRNA imaging in cells using fluorogenic templated Staudinger reaction between PNA-based probes / Katarzyna Gorska and Nicolas Winssinger; title: 16. Cellular delivery of peptide nucleic acids (PNAs) / Takehiko Shiraishi and Peter E. Nielsen; title: 17. Peptide nucleic acid-mediated recombination for targeted genomic repair and modification / Erica B. Schleifman and Peter M. Glazer; title: 18. Antisense effects of PNAs in bacteria / Shan Goh, Jem Stach, and Liam Good.