Primary and stem cells
Subtitle: gene transfer technologies and applications
By: edited by Uma Lakshmipathy and Bhaskar Thyagarajan
Edition: 1st ed.
Publisher: John Wiley and Sons
Published: 2011
Place of publication: Hoboken, N.J
Language: eng
ISBN-13: 9780470610749
Subjects: Gene Transfer Techniques; Genetic Engineering; Stem Cells; Transgenes
Description: "This book describes basic cell engineering methods, emphasizing stem cell applications, and use of the genetically modified stem cells in cell therapy and drug discovery. Together, the chapters introduce and offer insights on new techniques for engineering of stem cells and the delivery of transgenes into stem cells via various viral and non-viral systems. The book offers a guide to the types of manipulations currently available to create genetically engineered stem cells that suit any investigator's purpose, whether it's basic science investigation, creation of disease models and screens, or cells for therapeutic applications"--Provided by publisher.
Notes: Includes bibliographical references and index.
Table of contents: title: Machine generated contents note: Part 1: Cloning and Gene Delivery. Chapter 1: DNA assembly technologies based on homologus recombination / Billyana Tsvetanova ... [et al.]; title: Chapter 2: Multigene Assembly for Construction of Synthetic Operons / Takefumi Sone ... [et al.]; title: Chapter 3: Strategies for the delivery of Naked DNA / Jennifer C. Moore and Ronald P. Hart ... [et al.]; title: Part 2: Non-Integrating Technologies. Chapter 4: Episomal Vectors / Junying Yu; title: Chapter 5: Nonintegrating DNA virus / Chris Kemp and David Kuninger; title: Chapter 6: Nonintegrating RNA viruses / Noemi Fusaki; title: Chapter 7: Protein delivery / Xiao-Jian Han and Kazuhito and Tomizawa; title: Part 3: Integrating Technologies. Chapter 8: Sleeping Beauty Transposon-Mediated Stable Gene Delivery / Andrew Wilber ... [et al.]; title: Chapter 9: Integrating viral vectors for gene modifcations / Gemma L. Mendel, Brian Paszkiet, and Rafal P. Witek; title: Chapter 10: Bacteriophase integrases for site-specific integration / Bhaskar Thyagarajan; title: Chapter 11: Improving gene targeting efficiency in human pluripotent stem cells / Sangyoon Han ... [et al.]; title: Part 4: Applications. Chapter 12: Modifed stem cells as disease models and in toxicity screening / Catharina Ellerstrom ... [et al.]; title: Chapter 13: Screening and drug discovery / Elizabeth A. Davenport and Robert S. Ames.