The effect of synergistic ablation and heat shock on the rat plantaris muscle.
Published: 2004
Language: eng
Number of pages: 164
Description: The effect of synergistic ablation (SA+) and heat shock (HS+) on the rat plantaris muscle was investigated by measuring changes in HSP72, HSP25, proliferating cell nuclear antigen (PCNA), MyoD, myosin heavy chain type I (MHC I) content (mug), muscle mass (g) and protein concentration (mug/mg) over a 7-day period. Morphological changes and satellite cell expression patterns were determined histo- and immunohistochemically. The results demonstrated that HS+ prior to SA+ lead to elevated HSP72, decreased protein concentration and attenuated hypertrophy by preventing MHC I expression, whereas SA+ without HS+ results in a more gradual increase in HSP72, a similar HSP25 expression pattern as HS+ animals, reduced edema and increased MHC I protein accumulation. Satellite cell cycling was similar between HS+ and HS- plantaris muscles following SA+. It is suggested that the mechanism by which HS+ attenuates hypertrophy in the rat plantaris muscle is independent of satellite cell activation and replication.
Notes: Adviser: Marius Locke.

Thesis (M.Sc.)--University of Toronto, 2004.

Electronic version licensed for access by U. of T. users.

Source: Masters Abstracts International, Volume: 42-06, page: 2192.

MICR copy on microfiche (2 microfiches).