Pluripotent stem cells
Subtitle: methods and protocols
By: edited by Uma Lakshmipathy, Mohan C. Vemuri
Series: Methods in molecular biology -- 997; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 997.; Springer protocols (Series)
Publisher: Humana Press; Springer
Published: 2013
Place of publication: New York
Language: eng
Number of pages: 292
ISBN-13: 9781627033473; 9781627033480
Subjects: Stem cells; Research; Pluripotent Stem Cells; Laboratory Manuals; Cytological Techniques; Methods
Notes: Includes bibliographical references and index.

Sources and derivation of human embryonic stem cells / Michal Amit -- New chemical approach to the efficient generation of mouse embryonic stem cells / Hossein Baharvand and Seyedeh-Nafiseh Hassani -- Review of the methods for human iPSC derivation / Nasir Malik and Mahendra S. Rao -- Generation of human-induced pluripotent stem cells by lentiviral transduction / Jennifer C. Moore -- Generation of induced pluripotent stem cells with CytoTune, a non-integrating sendai virus / Pauline T. Lieu ... [et al.] -- Generation of human-induced pluripotent stem cells (hiPSCs) using episomal vectors on defined essential 8TM medium conditions / Andrew Fontes ... [et al.] -- Feeder-free substrates for pluripotent stem cell culture / Alexandria Sams and Mark J. Powers -- Methods for culturing human embryonic stem cells on feeders / Jasmeet Kaur and Mary Lynn Tilkins -- Methods for culturing human embryonic stem cells in a xeno-free system / Jasmeet Kaur ... [et al.] -- Directed differentiation of human pluripotent stem cells along the pancreatic endocrine lineage / Dennis Van Hoof and Muluye E. Liku -- Directed differentiation of pluripotent stem cells to functional hepatocytes / Philip Roelandt, Jolien Vanhove, and Catherine Verfaillie -- Highly efficient directed differentiation of human induced pluripotent stem cells into cardiomyocytes / Paul W. Burridge and Elias T. Zambidis -- Generation of transgene-free iPSC lines from human normal and neoplastic blood cells using episomal vectors / Kejin Hu and Igor Slukvin -- Cellular characterization of human pluripotent stem cells / Rene H. Quintanilla Jr. -- TaqMan® OpenArray® high-throughput transcriptional analysis of human embryonic and induced pluripotent stem cells / Sunali N. Patel ... [et al.] -- Epigenome analysis of pluripotent stem cells / Christopher L. Ricupero, Mavis R. Swerdel, and Ronald P. Hart -- Single cell gene expression analysis of pluripotent stem cells / Ronald V. Abruzzese and Richard A. Fekete -- Profiling stem cells using quantitative PCR protein assays / David Ruff and Pauline T. Lieu -- Labeled stem cells as disease models and in drug discovery / Catharina Ellerström, Raimund Strehl, and Johan Hyllner -- Cloning technologies / Andrew Fontes -- Stable transfection using episomal vectors to create modified human embryonic stem cells / Ying Liu, Kate Judd, and Uma Lakshmipathy -- Site-specific integration in human ESC using Jump-In (TM) TI(TM) technology  / Chad C. MacArthur.
Table of contents: title: Sources and derivation of human embryonic stem cells / Michal Amit; title: New chemical approach to the efficient generation of mouse embryonic stem cells / Hossein Baharvand and Seyedeh-Nafiseh Hassani; title: Review of the methods for human iPSC derivation / Nasir Malik and Mahendra S. Rao; title: Generation of human-induced pluripotent stem cells by lentiviral transduction / Jennifer C. Moore; title: Generation of induced pluripotent stem cells with CytoTune, a non-integrating sendai virus / Pauline T. Lieu ... [et al.]; title: Generation of human-induced pluripotent stem cells (hiPSCs) using episomal vectors on defined essential 8TM medium conditions / Andrew Fontes ... [et al.]; title: Feeder-free substrates for pluripotent stem cell culture / Alexandria Sams and Mark J. Powers; title: Methods for culturing human embryonic stem cells on feeders / Jasmeet Kaur and Mary Lynn Tilkins; title: Methods for culturing human embryonic stem cells in a xeno-free system / Jasmeet Kaur ... [et al.]; title: Directed differentiation of human pluripotent stem cells along the pancreatic endocrine lineage / Dennis Van Hoof and Muluye E. Liku; title: Directed differentiation of pluripotent stem cells to functional hepatocytes / Philip Roelandt, Jolien Vanhove, and Catherine Verfaillie; title: Highly efficient directed differentiation of human induced pluripotent stem cells into cardiomyocytes / Paul W. Burridge and Elias T. Zambidis; title: Generation of transgene-free iPSC lines from human normal and neoplastic blood cells using episomal vectors / Kejin Hu and Igor Slukvin; title: Cellular characterization of human pluripotent stem cells / Rene H. Quintanilla Jr.; title: TaqMan® OpenArray® high-throughput transcriptional analysis of human embryonic and induced pluripotent stem cells / Sunali N. Patel ... [et al.]; title: Epigenome analysis of pluripotent stem cells / Christopher L. Ricupero, Mavis R. Swerdel, and Ronald P. Hart; title: Single cell gene expression analysis of pluripotent stem cells / Ronald V. Abruzzese and Richard A. Fekete; title: Profiling stem cells using quantitative PCR protein assays / David Ruff and Pauline T. Lieu; title: Labeled stem cells as disease models and in drug discovery / Catharina Ellerström, Raimund Strehl, and Johan Hyllner; title: Cloning technologies / Andrew Fontes; title: Stable transfection using episomal vectors to create modified human embryonic stem cells / Ying Liu, Kate Judd, and Uma Lakshmipathy; title: Site-specific integration in human ESC using Jump-In (TM) TI(TM) technology  / Chad C. MacArthur.