Helicases
Subtitle: methods and protocols
By: edited by Mohamed M. Abdelhaleem
Series: Springer protocols; Methods in molecular biology -- 587; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 587.
Publisher: Humana Press
Published: 2010
Place of publication: New York, N.Y
Language: eng
Number of pages: 404
ISBN-13: 9781603273541; 9781603273558
Subjects: RNA Helicases; Laboratory Manuals; DNA helicases; DNA Helicases
Notes: Includes bibliographical references and index.
Table of contents: title: Helicases : an overview / Mohamed Abdelhaleem; title: Fluorescent biosensors to investigate helicase activity / Martin R. Webb; title: Single-molecule FRET analysis of helicase functions / Eli Rothenberg and Taekjip Ha; title: Kinetics of motor protein translocation on single-stranded DNA / Christopher J. Fischer, Lake Wooten, Eric J. Tomko, and Timothy M. Lohman; title: Experimental and computational analysis of DNA unwinding and polymerization kinetics / Manjula Pandey, Mikhail K. Levin, and Smita S. Patel; title: Protein displacement by helicases / Laxmi Yeruva and Kevin D. Raney; title: In vivo investigation of protein-protein interactions for helicases using tandem affinity purification / Matthew Jessulat, Terry Buist, Md Alamgir, Mohsen Hooshyar, Jianhua Xu, Hiroyuki Aoki, M. Clelia Ganoza, Gareth Butland, and Ashkan Golshani; title: Mapping genomic targets of DNA helicases by chromatin immunoprecipitation in Saccharomyces cerevisiae / Jennifer Cobb and Haico van Attikum; title: Methods to study how replication fork helicases unwind DNA / Daniel L. Kaplan and Irina Bruc; title: Simple enzymatic assays for the in vitro motor activity of transcription termination factor rho from Escherichia coli / Marc Boudvillain, Céline Walmacq, Annie Schwartz, and Frédérique Jacquinot; title: Single-molecule studies of RecBCD / Thomas T. Perkins and Hung-Wen Li; title: Mutational analysis of bloom helicase / Xu Guang Xi; title: In vivo analysis of Drosophila BLM helicase function during DNA double-strand gap repair / Mitch McVey; title: Purification and characterization of RecQ helicases of plants / Daniela Kobbe, Manfred Focke, and Holger Puchta; title: Fluorometric assay of Hepatitis C virus NS3 helicase activity / Mariusz Krawczyk, Anna Stankiewicz-Drogoń, Anne-Lise Haenni, and Anna Boguszewska-Chachulska; title: A method to simultaneously monitor hepatitis C virus NS3 helicase and protease activities / David N. Frick, Olya Ginzburg, and Angela M.L. Lam; title: Computer modeling of helicases using elastic network model / Wenjun Zheng; title: Duplex unwinding with DEAD-box proteins / Eckhard Jankowsk and, Andrea Putnam; title: Analysis of RNA helicase p68 (Ddx5) as a transcriptional regulator / Samantha M. Nicol and  Frances V. Fuller-Pace; title: A method to study the role of DDX3 RNA helicase in HIV-1 / Chia-Yen Chen, Venkat R.K. Yedavalli, and Kuan-Teh Jeang; title: Molecular characterization of nuclear DNA helicase II (RNA helicase A) / Suisheng Zhang and Frank Grosse; title: Regulation of inter- and intramolecular interaction of RNA, DNA, and proteins by MLE / Hyangyee Oh, Andrew M. Parrott, Yongkyu Park, and Chee-Gun Lee; title: Biochemical characterization of human Upf1 helicase / Zhihong Cheng, Gaku Morisawa, and Haiwei Song; title: Assays of the helicase, ATPase, and exoribonuclease activities of the yeast mitrochondrial degradosome / Michal Malecki, Piotr P. Stepien, and Pawel Golic; title: Characterization of the helicase activity and anti-telomerase properties of yeast Pif1p in vitro / Jean-Baptiste Boulé and Virginia A. Zakian; title: A method to confer salinity stress tolerance to plants by helicase overexpression / Narendra Tuteja; title: A method to inhibit the growth of Plasmodium falciparum by double-stranded RNA-mediated gene silencing of helicases / Renu Tuteja.