Protein affinity tags
Subtitle: methods and protocols
By: edited by Richard J. Giannone, Organic & Biological Mass Spectrometry, Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA, Andrew B. Dykstra, Analytical Sciences, Amgen, West Greenwich, RI, USA
Series: Methods in molecular medicine -- 1177; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 1177.; Springer protocols (Series)
Publisher: Humana Press
Published: 2014
Place of publication: New York
Language: eng
Number of pages: 213
ISBN-13: 9781493910335; 9781493910342
Copyright date: 2014
Subjects: Recombinant Proteins; Isolation & purification; Affinity labeling; Methods; Laboratory manuals; Proteins; Purification; Affinity Chromatography; Laboratory Manuals
Notes: Includes bibliographical references and index.
Table of contents: title: 1D4 : a versatile epitope tag for the purification and characterization of expressed membrane and soluble proteins  -- Laurie L. Molday and Robert S. Molday; title: Affinity purification of heme-tagged proteins  -- Wesley B. Asher and Kara L. Bren; title: Purification of a recombinant protein with cellulose-binding module 3 as the affinity tag  -- Dongmei Wang and Jiong Hong; title: Purification of E. coli proteins using a self-cleaving chitin-binding affinity tag  -- Michael J. Coolbaugh and David W. Wood; title: Simplified protein purification using an autoprocessing, inducible enzyme tag  -- Aimee Shen; title: SUMO as a solubility tag and in vivo cleavage of SUMO fusion proteins with UlP1  -- Dennis Kuo, Minghua Nie, and Albert J Courey; title: Rescuing aggregation-prone proteins in Escherichia coli with a dual His6-MBP tag  -- Danielle Needle and David S. Waugh; title: Expression, purification, and immobilization of recombinant tamavidin 2 fusion proteins -- Yashimitsu Takakura, Naomi Oka, and Masako Tsunashima; title: Use of tandem affinity chromatography for purification of cannabinoid receptor CB2  -- Silvia C. Locatelli-Hoops and Alexei A. Yeliseev; title: Detection of protein-protein interactions using tandem affinity purification  -- Ian Goodfellow and Dalan Bailey; title: An improved in vivo biotinylation strategy combined with FLAG and antibody based approaches for affinity purification of protein complexes in mouse embryonic stem cells  -- Francesco Faiolo [and three others]; title: Purification of recombinant proteins with a multifunctional GFP tag  -- Takashi Murayama and Takuya Kobayashi; title: Targeted purification of SnAvi-tagged proteins  -- Ursula Schäffer, Ralf Baumeister, and Ekkehard Schulze; title: An efficient fluorescent protein-based multifunctional affinity purification approach in mammalian cells  -- Hanhui Ma [and three others]; title: Bimolecular affinity purification : a variation of TAP with multiple applications -- Petro Starokadomskyy and Ezra Burstein.