Protein-ligand interactions
Subtitle: methods and applications
By: edited by G. Ulrich Nienhaus
Contributors: Nienhaus, G. Ulrich
Series: Methods in molecular biology -- 305; Methods in molecular biology (Clifton, N.J.) -- 305
Publisher: Humana Press
Published: 2005
Place of publication: Totowa, N.J
Language: eng
Number of pages: 568
Subjects: Protein binding -- Laboratory manuals; Ligands -- Laboratory manuals
Genres: Laboratory manuals
Description: A readily reproducible collection of established and emerging techniques for studying the interaction between proteins and ligands, including biochemical/bulk techniques, structure analysis, spectroscopy, single-molecule studies, and theoretical/computational tools. Among the highlights are surface plasmon resonance (SPR) and reflectometric biosensor approaches, high-throughput screening with confocal optics microscopy, single molecule fluorescence and fluorescence correlation spectroscopy (FCS), atomic force microscopy (AFM), crystallography of reaction intermediates, and time-resolved x-ray crystallography. The protocols follow the Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Notes: Includes bibliographical references and index
Table of contents: title: Isothermal titration calorimetry; title: Direct optical detection of protein-ligand interactions; title: Label-free detection of protein-ligand interactions by the quartz crystal microbalance; title: Measurement of solvent accessibility at protein-protein interfaces; title: Hydrophobic interaction chromatography: harnessing multivalent protein-surface interactions for purification procedures; title: Sedimentation velocity method in the analytical ultracentrifuge for the study of protein-protein interactions; title: Protein-ligand interaction probed by time-resolved crystallography; title: X-ray crystallography of protein-ligand interactions; title: Combined use of XAFS and crystallography for studying protein-ligand interactions; title: Probing heme protein-ligand interactions by UV/visible absorption spectroscopy; title: Ultrafast time-resolved IR studies of protein-ligand interactions; title: Monitoring protein-ligand interactions by time-resolved FTIR difference spectroscopy; title: Proteins in motion: resonance raman spectroscopy as a probe of functional intermediates; title: Fluorescence polarization/anisotropy approaches to study protein-ligand interactions: effects of errors and uncertainties; title: Ligand binding with stopped-flow rapid mixing; title: Circular dichroism spectroscopy for the study of protein-ligand interactions; title: High throughput screening of interactions between G protein-coupled receptors and ligands using confocal optics microscopy; title: Single-molecule study of protein-protein and protein-DNA interaction dynamics; title: Application of fluorescence correlation spectroscopy to hapten-antibody binding; title: Atomic force microscopy measurements of protein-ligand interactions on living cells; title: Computer simulation of protein-ligand interactions: challenges and applications; title: Force probe molecular dynamics simulations; title: Study of ligand-protein interactions by means of density functional theory and first-principles molecular dynamics