The role of the epithelial-specific ETS factor, Ese-2, in the regulation of cytokeratin 18 gene expression in pulmonary epithelial cells.
Published: 2005
Language: eng
Number of pages: 113
Description: The role of Ese-2, an Ets family transcription factor, in gene regulation is not known. In this study, the interaction between Ese-2 and cytokeratin 18 (K18) intron 1 was characterized in lung epithelial cells. Reporter gene assays showed Ese-2 was able to upregulate K18 intron 1 and reporter gene expression by approximately 2-fold. It was also established that full length Ese-2 did not bind DNA strongly, therefore truncated versions of the protein, containing the ETS domain or the Pointed domain, were created and tested in electrophoresis mobility shift assays. Multiple interactions between the ETS domain and putative DNA binding sites within K18 intron 1 were observed, which led to the determination of a possible Ese-2 DNA binding consensus sequence. These experiments suggest that Ese-2 could play a role in the regulation of K18 expression in lung epithelial cells.
Notes: Source: Masters Abstracts International, Volume: 44-01, page: 0254.

Thesis (M.Sc.)--University of Toronto, 2005.

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