Immunoelectron microscopy
Subtitle: methods and protocols
By: edited by Steven D. Schwartzbach, Tetsuaki Osafune
Series: Methods in molecular biology -- 657; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- 657.; Springer protocols
Publisher: Humana Press
Published: 2010
Place of publication: New York
Language: eng
Number of pages: 352
ISBN-13: 9781607617822; 9781607617839
Subjects: Electron microscopy; Immunohistochemistry; Methods; Instrumentation; Technique; Immunoelectron Microscopy
Notes: Includes bibliographical references and index.

Also available online.
Table of contents: title: Protein antigen expression in escherichia coli for antibody production  -- D.M. Rancour, S.K. Backues and S.Y. Bednarek; title: Expression of epitope-tagged proteins in plants  -- T. Furuichi; title: Expression of epitope-tagged proteins in arabidopsis leaf mesophyll protoplasts  -- Y.H. Cho and S.D. Yoo; title: Transient expression of epitope-tagged proteins in mammalian cells  -- M.L. Styers, J. Lowery and E. Sztul; title: Production and purification of polyclonal antibodies  -- M. Nakazawa, M. Mukumoto and K. Miyatake; title: Production and purification of monoclonal antibodies  -- M. Nakazawa, M. Mukumoto and K. Miyatake; title: Production of antipeptide antibodies  -- B.S. Lee ... [et al.]; title: Preparation of colloidal gold particles and conjugation to protein A, IgG, F(ab')₂, and streptavidin  -- S. Yokota; title: Immunoelectron microscopy of chemically fixed developing plant embryos  -- T. Osafune and S.D. Schwartzbach; title: Pre-embedding immunogold localization of antigens in mammalian brain slices  -- T. Schikorski; title: Pre-embedding immunoelectron microscopy of chemically fixed mammalian tissue culture cells  -- H. Hagiwara ... [et al.]; title: Immunoelectron microscopy of cryofixed and freeze-substituted plant tissues  -- M. Takeuchi, K. Takabe and Y. Mineyuki; title: In vivo cryotechniques for preparation of animal tissues for immunoelectron microscopy  -- S. Ohno ... [et al.]; title: Immunoelectron microscopy of cryofixed freeze-substituted mammalian tissue culture cells  -- A. Sawaguchi; title: Immunoelectron microscopy of cryofixed freeze-substituted saccharomyces cerevisiae  -- J. Fiserova and M.W. Goldberg; title: High-resolution molecular localization by freeze-fracture replica labeling  -- A. Fujita and T. Fujimoto; title: Pre-embedding electron microscopy methods for glycan localization in chemically fixed mammalian tissue using horseradish peroxidase-conjugated lectin  -- Y. Akimoto and H. Kawakami; title: Pre-embedding nanogold silver and gold intensification  -- A. Yamamoto and R. Masaki; title: The post-embedding method for immunoelectron microscopy of mammalian tissues: A standardized procedure based on heat-induced antigen retrieval  -- S. Yamashita; title: Double-label immunoelectron microscopy for studying the colocalization of proteins in cultured cells  -- H. Hagiwara ... [et al.]; title: Serial section immunoelectron microscopy of algal cells  -- T. Osafune and S.D. Schwartzbach; title: Freeze-etch electron tomography for the plasma membrane interface  -- N. Morone; title: Localization of rDNA at nucleolar structural components by immunoelectron microscopy  -- S. Sato and Y. Sato; title: Immunogold labelling for scanning electron microscopy  -- M.W. Goldberg and J. Fiserova; title: Horseradish peroxidase as a reporter gene and as a cell-organelle-specific marker in correlative light-electron microscopy  -- T. Schikorski; title: Monitoring rapid endocytosis in the electron microscope via photoconversion of vesicles fluorescently labeled with FM1-43 -- T. Schikorski.