Vitamins and coenzymes.
By: edited by Donald B. McCormick, John W. Suttie, Conrad Wagner.
Contributors: McCormick, Donald B.; Suttie, J. W. 1934-; Wagner, Conrad.
Series: Methods in enzymology -- v. 282.
Publisher: Academic Press
Published: 1997
Place of publication: San Diego
Language: eng
Number of pages: 505
Subjects: Vitamins.; Coenzymes.; Enzymology.
Notes: Includes bibliographical references and indexes.
Table of contents: value: Generation and characterization of cellular retinoic acid-binding proteins from Escherichia coli expression systems; value: Generation and characterizing retinoid receptors from Escherichia coli and insect cell expression systems; value: Expression and characterization of retinoid receptors in yeast; value: Use of in situ hybridization techniques to study embryonic expression of retinoid receptors and binding proteins; value: Use of quantitative polymerase chain reaction to study retinoid receptor expression; value: Use of quantitative polymerase chain reaction to study cellular retinoic acid-binding protein-II mRNA expression; value: Use of transgenic mice to study activation of retinoic acid-responsive promoters; value: Use of transgenic mice to eliminate retinoic acid receptor function; value: Use of reporter cells to study endogenous retinoid sources; value: Preparation of radiolabeled 9-cis- and all-trans-retinoids; value: Identification and quantification of retinoic acid and other metabolites from Ý-Carotene excentric cleavage; value: Assessing metabolism of Ý-Carotene.; value: Atmospheric pressure chemical ionization and electron capture negative chemical ionization mass spectrometry in studying Ý-Carotene conversion to retinol in humans; value: Synthesis of Vitamin D₃ and 1Ü,25-Dihydroxy Vitamin D₃; value: Assay of 1,25-dihydroxyvitamin D₃ from serum samples; value: Quantitation of 25-Hydroxyvitamin D and 1,25-Dihydroxyvitamin D; value: Assay of vitamin D derivatives and purification of vitamin D hydroxylases; value: Assay of 25-Hydroxyvitamin D 1Ü-Hydroxlase and 24-Hydroxylase; value: Molecular cloning of vitamin D₃ hydroxylases; value: Role of 1Ü,25-dihydroxyvitamin D₃ in osteoclast differentiation and function; value: Assay of direct effect of 1,25-Dihydroxyvitamin D₃ on calcium ion; value: Vitamin E status and immune function; value: Inhibition of platelet adhesion as functional test for vitamin E status; value: Inhibition of plasma cholesterol ester hydroperoxide; value: Ü-Tocopherol-binding proteins; value: Ü-Carboxyethyl 6-Hydrozychroman.; value: Purification of vitamin K-dependent carboxylase from cultured cells; value: Purification of native bovine carboxylase; value: Purification of Þ-Glutamyl carboxylase; value: Assay of vitamin K-dependent carboxylase activity; value: Expression of human anticoagulation protein C and Þ-Carboxyglutamic acid mutants; value: Determination of site-specific Þ-carboxyglutamin acid formation; value: Purification of warfarin-sensitive vitamin K epoxide reductase; value: Determination of vitamin K compounds in plasma or serum; value: Assay of phylloquinone in plasma; value: Assay of menaquinones in plasma; value: Assay of phylloquinone and menaquinones in human liver; value: Determination of phylloquinone in foods; value: Assay of menaquinones in bacterial cultures, stool samples, and intestinal contents.