Cell fusion
Subtitle: overviews and methods
By: edited by Kurt Pfannkuche
Edition: 2nd edition.
Series: Methods in molecular biology -- 1313; Methods in molecular biology (Clifton, N.J.) -- v. 1313.
Publisher: Humana Press; Springer
Published: 2015
Place of publication: New York
Language: eng
Number of pages: 248
ISBN-13: 9781493927036; 9781493927029
Copyright date: 2015
Subjects: Cell hybridization; Molecular biology; Cell Fusion; Methods; Cellular biology (cytology); Science; Life Sciences; Biology; Molecular Biology
Description: This volume details protocols on detection and analysis of fusion events in various cell types in vitro and in vivo. Additional, protocols aim to facilitate the future highly relevant process of engineering cells for specific purposes, such as generation of transgenic embryos and development of hybrid cells, in order to tackle specific tasks in cell biology and medicine. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Cell Fusion: Overviews and Methods, Second Edition serves as an ideal reference on cell fusion and laboratory protocols.
Notes: Includes bibliographical references and index.
Table of contents: title: State of the art in cell-cell fusion  -- Lena Willkomm and Wilhelm Bloch; title: Fusion in cancer : an explanatory model for aneuploidy, metastasis formation, and drug resistance  -- Marieke Mohr, Kurt S. Zaenker, and Thomas Dittmar; title: Mouse embryos' fusion for the tetraploid complementation assay  -- Marina Gertsenstein; title: Generation of mouse chimeras with high contribution of tetraploid embryonic stem cells and embryonic stem cell-fibroblast hybrid cells  -- Natalia M. Matveeva, ElenaA. Kizilova, and Oleg L. Serov; title: Microfluidic systems for cell pairing and fusion  -- Burak Duraand Joel Voldman; title: Chromosome tracking in fused cells by single nucleotide polymorphisms  -- Azra Fatima, Jürgen Hescheler, and Tomo Šarić; title: Fusion of mesenchymal stem cells and islet cells for cell therapy  -- Shoichiro Sumi and Goichi Yanai; title: Detection of fusion events in mammalian skeletal muscle  -- Frank Suhr; title: FISH detection of X and Y chromosomes in combination with immunofluorescence to study contribution of transplanted cells to skeletal muscle fibers  -- Anna Strömberg and Monika Jansson; title: Using phosphatidylserine exposure on apoptotic cells to stimulate myoblast fusion  -- Amelia E. Hochreiter-Hufford, Sanja Arandjelovic, and Kodi S. Ravichandran; title: Tracing myoblast fusion in Drosophila embryos by fluorescent actin probes  -- Shruti Haralalka and Susan M. Abmayr; title: Analyzing cell fusion events within the central nervous system using bone marrow chimerism  -- Kevin Kemp and Kelly Hares; title: Cell fusion between dendritic cells and whole tumor cells  -- Shigeo Koidoand Jianlin Gong; title: Membrane nanotube formation in osteoclastogenesis  -- Toshio Kukita [and others]; title: Modified adherence method (MAM) for electrofusion of anchorage-dependent cells  -- Marko Ušajand Maša Kandušer; title: FRET in the analysis of in vitro cell-cell fusion by flow cytometry  -- Guillermo Gómez-Icazbalceta [and others]; title: Dual split protein (DSP) assay to monitor cell-cell membrane fusion  -- Shuhei Nakane and Zene Matsuda; title: Photoconvertible fluorescent protein-based live imaging of mitochondrial fusion -- Geoffrey C. Owens and David B. Edelman.