Handbook of ELISPOT
Subtitle: methods and protocols
By: edited by Alexander E. Kalyuzhny
Edition: 2nd ed.
Series: Springer protocols; Methods in molecular biology -- 792; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 792.
Publisher: Humana Press
Published: 2012
Place of publication: New York
Language: eng
Number of pages: 261
ISBN-13: 9781617793240; 9781617793257
Subjects: Immunology; Methods; Enzyme-Linked Immunospot Assay; Enzyme-linked immunosorbent assay; T-Lymphocytes; Laboratory Manuals; Cytokines; Cellular Immunity
Other titles: Handbook of enzyme-linked immunospot assay
Notes: Includes bibliographical references and index.
Table of contents: title: Unique strengths of ELISPOT for T cell diagnostics / Paul V. Lehmann and Wenji Zhang; title: The impact of harmonization on ELISPOT assay performance / Sylvia Janetzki and Cedrik M. Britten; title: Equine ELISPOT assay to study secretion of IFN[gamma] and IL-4 from peripheral blood mononuclear cells / Jodi Hagen ... [et al.]; title: Utilization of feline ELISPOT for mapping vaccine epitopes / Jeffrey R. Abbott ... [et al.]; title: Analyzing cellular immunity to AAV in a canine model using ELISPOT assay / Zejing Wang ... [et al.]; title: Dual- and triple-color fluorospot / Niklas Ahlborg and Bernt Axelsson; title: ELISPOT assay as a tool to study oxidative stress in lymphocytes / Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny; title: ELISPOT assay for neuroscience research : studying TNF[alpha] secretion from microglial cells / Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny; title: ELISPOT assay as a tool to study the effects of stem cells on cytokine secretion / Jun-Seop Shin and Chung-Gyu Park; title: Combining ELISPOT and ELISA to measure amounts of cytokines secreted by a single cell / Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny; title: How ELISPOT morphology reflects on the productivity and kinetics of cells' secretory activity / Alexey Y. Karulin and Paul V. Lehmann; title: Mathematical algorithms for automatic search, recognition, and detection of spots in ELISPOT assay / Sergey S. Zadorozhny and Nikolai N. Martynov; title: Objective, user-independent ELISPOT data analysis based on scientifically validated principles / Wenji Zhang and Paul V. Lehmann; title: Statistical analysis of ELISPOT assays / Marcus Dittrich and Paul V. Lehmann; title: Response determination criteria for ELISPOT : toward a standard that can be applied across laboratories / Zoe Moodie ... [et al.]; title: Detection of Vaccinia virus-specific IFN[gamma] and IL-10 secretion from human PBMCs and CD8⁺ T cells by ELISPOT / Benjamin J. Umlauf ... [et al.]; title: ELISPOT assays to enumerate bovine IFN-[gamma]-secreting cells for the development of novel vaccines against bovine tuberculosis / Martin Vordermeier and Adam O. Whelan; title: IL-7 addition increases spot size and number as measured by T-SPOT.TB® / Marsha L. Feske ... [et al.]; title: Overview of membranes and membrane plates used in research and diagnostic ELISPOT assays / Alan J. Weiss.