Ubiquitin-proteasome protocols
By: edited by Cam Patterson, Douglas M. Cyr
Contributors: Patterson, Cam; Cyr, Douglas M
Series: Methods in molecular biology -- 301; Methods in molecular biology (Clifton, N.J.) --  301
Publisher: Humana Press
Published: 2005
Place of publication: Totowa, N.J
Language: eng
Number of pages: 381
Subjects: Ubiquitin -- Laboratory manuals; Proteolytic enzymes -- Laboratory manuals
Genres: Laboratory manuals
Notes: Includes bibliographical references and index
Table of contents: title: Small-molecule inhibitors of proteasome activity / Maria Gaczynska and Pawel A. Osmulski; title: Purification of E1 and E1-like enzymes / Arthur L. Haas; title: Assays for RING family ubiquitin ligases / Manabu Furukawa, Paul S. Andrews, and Yue Xiong; title: Ubiquitin chain synthesis / Shahri Raasi and Cecile M. Pickart; title: Purification of proteasomes, proteasome subcomplexes, and proteasome-associated proteins from budding yeast / David S. Leggett, Michael H. Glickman, and Daniel Finley; title: Recognition and processing of misfolded proteins by PA700, the 19S regulatory complex of the 26S proteasome / Chang-Wei Liu ... [et al.]; title: Cell-free assay for ubiquitin-independent proteasomal protein degradation / Chaim Kahana and Yuval Reiss; title: Assays of proteasome-dependent cleavage products / Stefan Tenzer and Hansjörg Schild; title: Identification of components of protein complexes / Carol E. Parker ... [et al.]; title: Mass spectrometric determination of protein ubiqitination / Carol E. Parker ... [et al.]; title: Reconstitution of endoplasmic reticulum-associated degradation using yeast membranes and cytosol / Robert J. Lee, Ardythe A. McCracken, and Jeffrey L. Brodsky; title: Reticulocyte lysate as a model system to study endoplasmic reticulum membrane protein degradation / Eric Carlson ... [et al.]; title: Deubiquitinating enzyme purifucation, assay inhibitors, and characterization / Nathaniel S. Russell and Keith D. Wilkinson; title: Measuring ubiquitin conjugation in cells / Edward G. Mimnaugh and Leonard M. Neckers; title: Assays for proteasome assembly and maturation / R. Jürgen Dohmen ... [et al.]; title: N-terminal ubiquitination / Aaron Ciechanover; title: Quantitating defective ribosome products / Shu-Bing Qian, Jack R. Bennink, and Jonathan W. Yewdell; title: endoplasmic reticulum-associated protein quality control and degradation: screen for ERAD mutants after ethylmethane sulfonate mutagenesis / Antje Schäfer and Dieter H. Wolf; title: Endoplasmic reticulum-associated protein quality control and degradation: genome wide screen for ERAD components / Antje Schäfer and Dieter H. Wolf; title: Cystic fibrosis transmembrane conductance regulator as a model substrate to study endoplasmic reticulum protein quality control in mammalian cells / J. Michael Younger ... [et al.]; title: Aggresome formation / Michael J. Corboy, Philip J. Thomas, and W. Christian Wigley; title: Detection of sumoylated proteins / Roland S. Hilgarth and Kevin D. Sarge; title: Proteasome inhibitors in cancer therapy / Robert Z. Orlowski; title: Parkinson's disease: assays for the ubiquitin ligase activity of neural parkin / Michael G. Schlossmacher and Hideki Shimura