Biothermodynamics
By: edited by Michael L. Johnson, Jo M. Holt and Gary K. Ackers
Contributors: ScienceDirect (Online service)
Edition: 1st ed.
Series: Methods in enzymology -- v. 488
Publisher: Elsevier/Academic Press
Published: 2011
Place of publication: Amsterdam [Netherlands]; Boston [Mass.]
Language: eng
Format: [electronic resource] /
ISBN-13: 9780123812698; 9780123812681
Subjects: Life Sciences; Biochemistry; Protein Binding; Protein-protein interactions; Ligands (Biochemistry); Thermodynamics; SCIENCE; Protein binding; Ligands; Hemoglobins
Description: The use of thermodynamics in biological research can be equated to an energy book-keeping system. While the structure and function of a molecule is important, it is equally important to know what drives the energy force. This volume presents sophisticated methods for estimating the thermodynamic parameters of specific protein-protein, protein-DNA and small molecule interactions. Elucidates the relationships between structure and energetics and their applications to molecular design, aiding researchers in the design of medically important molecules.
Notes: Includes bibliographical references and indexes.

Description based on print version record.
Table of contents: title: 1.; title: Measurement and analysis of equilibrium binding titrations : a beginner's guide  -- Dorothy Beckett -- 2.; title: Macromolecular competition titration method : accessing thermodynamics of the unmodified macromolecule-ligand interactions through spectroscopic titrations of fluorescent analogs  -- Wlodzimierz Bujalowksi and Maria J. Jezewska -- 3.; title: Analysis of PKR-RNA interactions by sedimentation velocity  -- C. Jason Wong, Katherine Launer-Felty, and James L. Cole -- 4.; title: Structural and thermodynamic analysis of PDZ-ligand interactions  -- Tyson R. Shepherd and Ernesto J. Fuentes -- 5.; title: Thermodynamic analysis of metal ion-induced protein assembly  -- Andrew B. Herr and Deborah G. Conrady -- 6.; title: Thermodynamic dissection of colicin interactions  -- Nicholas G. Housden and Colin Kleanthous -- 7.; title: Energetics of Src homology domain interactions in receptor tyrosine kinase-mediated signaling  -- John E. Ladbury and Stefan T. Arold -- 8.; title: Structural and functional energetic linkages in allosteric regulation of muscle pyruvate kinase  -- J. Ching Lee and Petr Herman -- 9.; title: Analysis of free energy versus temperature curves in protein folding and macromolecular interactions  -- Vince J. LiCata and Chin-Chi Liu -- 10.; title: Application of the sequential n-step kinetic mechanism to polypeptide translocases  -- Aaron L. Lucius, Justin M. Miller, and Burki Rajendar -- 11.; title: A coupled equilibrium approach to study nucleosome thermodynamics  -- Andrew J. Andrews and Karolin Luger -- 12.; title: Quantitative methods for measuring DNA flexibility in vitro and in vivo -- Justin P. Peters, Nicole A. Becker, Emily M. Rueter, Zeljko Bajzer, Jason D. Kahn, and L. James Maher III.