Steroid receptors
Subtitle: methods and protocols
By: edited by Gabriella Castoria, Second University of Naples, Naples, Italy, Ferdinando Auricchio, Second University of Naples, Naples, Italy
Series: Methods in molecular biology -- 1204; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 1204.; Springer protocols (Series)
Publisher: Humana Press; Springer
Published: 2014
Place of publication: New York
Language: eng
Number of pages: 245
ISBN-13: 9781493913459; 9781493913466
Copyright date: 2014
Subjects: Steroid hormones; Cancer; Endocrine aspects; Laboratory Manuals; Steroid Receptors; Laboratory manuals; Receptors
Description: "Steroid Receptors: Methods and Protocols presents a selection of techniques that have been recently applied to the analysis of steroid receptors, powerful tools for the advancement of our understanding of both the mechanisms regulating gene transcription and the rapid signaling responses of tissues to signals. Research in this area has generated a wealth of data allowing the elucidation of steroid receptor mechanisms and improving the treatment of many endocrine disorders, above all cancers. Chapters cover methods to analyze gene transcription, chromatin and proteomic modifications, extra-nuclear signaling regulation, development of cell and animal models, and preparation of new antibodies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls."--Publisher's description.
Notes: Includes bibliographical references and index.
Table of contents: title: Progesterone receptor interaction with chromatin  -- Guillermo P. Vicent [and others]; title: Mapping the genomic binding sites of the activated retinoid X receptor in murine bone marrow-derived macrophages using chromatin immunoprecipitation sequencing  -- Bence Daniel, Balint L. Balint, Zsuzsanna S. Nagy, and Laszlo Nagy; title: Analysis of chromatin-nuclear receptor interactions by laser-chromatin immunoprecipitation  -- Rosaria Benedetti [and others]; title: Examining estrogen regulation of cancer stem cells through multicolor lineage tracing  -- Yongshu Zhang, Gabriel Eades, and Qun Zhou; title: Reporter mice for the study of long-term effects of drugs and toxic compounds  -- Nicoletta Rizzi [and others]; title: Analysis of histone posttranslational modifications in the control of chromatin plasticity observed at estrogen-responsive sites in human breast cancer cells  -- Annalisa Di Santi [and others]; title: In silico analysis of genomic data for construction of nuclear receptor network  -- Yun-Young Park and Ju-Seog Lee; title: Cofactor profiling of the glucocorticoid receptor from a cellular environment  -- Sofie J. Desme [and others]; title: Paxillin and steroid signaling : from frog to human  -- Stephen R. Hammes, Susanne U. Miedlich, and Aritro Sen; title: Analysis of the androgen receptor/filamin a complex in stromal cells  -- Pia Giovannelli [and others]; title: Multi-well plate immunoassays for measuring signaling protein activations/deactivations and membrane vs. intracellular receptor levels  -- Cheryl S. Watson [and others]; title: Proximity ligation assay to detect and localize the interactions of ER-alpha with PI3-K and Src in breast cancer cells and tumor samples  -- Coralie Poulard [and others]; title: Phosphoinositide 3-kinase assay in breast cancer cell extracts  -- Antonio Bilancio and Antimo Migliaccio; title: Rapid estrogen effects on aromatase phosphorylation in breast cancer cells  -- Stefania Catalano, Ines Barone, and Sebastiano Andò; title: Mouse monoclonal antibodies against estrogen receptor  -- Caterina De Rosa, Valentina Rossi, and Ciro Abbondanza; title: Analysis of estrogen receptor-beta interacting proteins using pull-down assay and MALDI-MS methods  -- Mahendra Kumar Thakur and Vijay Paramanik; title: Analysis of the conformation of the androgen receptor in spinal bulbar muscular atrophy by atomic force microscopy  -- Tobias Jochum and Andrew C.B. Cato; title: Imaging of corticosteroid receptors in live cells  -- Mayumi Nishi; title: Physiological techniques in the study of rapid aldosterone effects  -- Yamil R. Yusef, Warren Thomas, and Brian J. Harvey; title: Detection of the glucocorticoid receptors in brain protein extracts by SDS-PAGE -- Fernanda Marques [and others].