Arthritis research
Subtitle: methods and protocols
By: edited by Shunichi Shiozawa, Department of Rheumatology, Kyushu University, Beppu, Japan
Edition: Second edition.
Series: Methods in molecular medicine -- 1142; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 1142,; Springer protocols (Series)
Publisher: Humana Press
Published: 2014
Place of publication: New York
Language: eng
Number of pages: 204
ISBN-13: 9781493904037; 9781493904044
Copyright date: 2014
Subjects: Arthritis; Laboratory Manuals; Research
Description: Arthritis Research: Methods and Protocols, Second Edition expands upon the first edition to present new and current techniques for the research of arthritis and related conditions. A compendium of leaders in the field contribute chapters that cover practical research methods such as the intravital multiphoton microscopy technique, techniques for evaluating exhausted CD8 T cell and for studying nucleic acid sensors and their effects, methods for in vivo tetracycline-controlled transgenic mice and T cell receptor transgenic mice, protocols to detect V(D)J recombination products and microRNA, and the technique to make bleomycin-induced dermal fibrosis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Arthritis Research: Methods and Protocols, Second Edition will serve both professionals and novices with state-of-the-art techniques pertaining to this fascinating research field.
Notes: Previous edition: 2007.

Includes bibliographical references and index.
Table of contents: title: Intravital Multiphoton Microscopy for Dissecting Cellular Dynamics in Arthritic Inflammation and Bone Destruction --; title: Monitoring Multifunctionality of Immune-Exhausted CD8 T in Cancer Patients --; title: Characterization of Innate Immune Signalings Stimulated by Ligands for Pattern Recognition Receptors --; title: Principled for the Use of in vivo Transgene Techniques: Overview and an Introductory Practical Guide for the Selection of Tetracycline-Controlled Transgenic Mice --; title: Unraveling Autoimmunity with the Adoptive Transfer of T Cells from TCR-Transgenic Mice --; title: In Vivo Cell Transfer Assay to Detect Autoreactive T Cell Subsets --; title: Characterization of microRNAs and Their Targets --; title: Studies on the T Cell Receptor (TCR) Revision of Autoantibody-Inducing CD4T (aiCD4 T) Cell --; title: Basic Techniques for Studies of iNKT Cells and MAIT Cells --; title: Induction of de novo Autoimmune Disease in Normal Mice Upon Repeated Immunization with Antigen --; title: Mouse Models of Experimental Dermal Fibrosis: The Bleomycin-Induced Dermal Fibrosis --; title: Screening for Novel Serum Biomarker for Monitoring Disease Activity in Rheumatoid Arthritis Using iTRAQ Technology Based Quantitative Proteomic Approach --; title: Genome-Wide Genetic Study in Autoimmune Disease Prone Mice --; title: Bayesian Systems-Based Genetic Association Analysis with Effect Strength Estimation and Omic Wide Interpretation: A Case Study in Rheumatoid Arthritis --; title: Sample Processing, Protocol and Statistical Analysis of the Time of Flight Secondary Ion Mass Spectrometry (ToF-SIMS) of Protein, Cell and Tissue Samples --; title: Label-Free Imaging of Adipogenesis by Coherent Anti-Stokes Raman Scattering Microscopy.