Signal Transduction Protocols (Methods in Molecular Biology) (Methods in Molecular Biology)
Contributors: Robert C. Dickson (Editor); Michael D. Mendenhall (Editor)
Edition: 2 edition
Publisher: Humana Press
Published: June 18, 2004
Language: eng
Format: Hardcover
Number of pages: 327
ISBN-13: 9781588292452
Subjects: Clinical & Internal Medicine; Science/Mathematics; Laboratory manuals; G proteins; Science; Medical / Nursing; Life Sciences - Biochemistry; General; Life Sciences - Biology - Molecular Biology; Science / Cytology; Cellular signal transduction; Cell receptors; GTP-Binding Proteins
First sentence: A wide variety of methods used in the study of signal transduction in eukaryotes rely on the ability to precipitate proteins from whole cell extracts.
Description: Updated with many of the new approaches that have been transforming the field, including readily reproducible immunoassays, fluorescence-based assays, high-throughput methods, protein modification assays, lipid second messenger assays, and chromatin immunoprecipitation techniques. The book offers step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necesary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.