Gene expression profiling of microsatellite unstable and microsatellite stable colon cancers
Subtitle: the use of microarrays to study colon cancer.
Published: 2004
Language: eng
Number of pages: 135
Description: The global gene expression profiles of 26 colon cancer cell lines was examined by cDNA microarrays spotted with 19,200 genes and expressed sequenced tags (ESTs). Hierarchical clustering and principal component analysis (PCA) revealed two major subgroupings, which corresponded to MSI status. Statistical analysis of microarrays (SAM) analysis identified a list of 123 unique genes and ESTs showing significant differential expression between microsatellite stable (MSS) and microsatellite unstable (MSI) colon cancer cell lines, with a false discovery rate (FDR) of 7.66%. Four of these genes, RAB21, JAK1, CC5 and smgGDS were validated at the RNA level with real time PCR. Of the 123 unique genes and ESTs differentially expressed between the two groups, many genes have been previously implicated in cancer, including some specifically implicated in colon cancer. The microarray analysis also identified genes that have not been previously associated with cancer. Some of the genes may be candidate targets for new therapies and diagnostic/prognostic markers.
Notes: Adviser: S. Gallinger.

Thesis (M.Sc.)--University of Toronto, 2004.

Electronic version licensed for access by U. of T. users.

Source: Masters Abstracts International, Volume: 42-06, page: 2105.

MICR copy on microfiche (2 microfiches).