Plant secondary metabolism engineering
Subtitle: methods and applications
By: edited by Arthur Germano Fett-Neto
Series: Springer protocols; Methods in molecular biology -- 643
Publisher: Springer/Humana Press
Published: 2010
Place of publication: New York, NY
Language: eng
Number of pages: 339
ISBN-13: 9781607617228; 9781607617235
Subjects: Plants; Secondary Metabolism; Effect of stress on; Biochemical engineering; Methods; Biotransformation (Metabolism); Research; Biotransformation; Bioengineering; Laboratory manuals; Metabolism; Laboratory Manuals
Notes: Includes bibliographical references and index.
Table of contents: title: Plant secondary metabolism and challenges in modifying its operation : an overview / Naíla Cannes do Nascimento and Arthur Germano Fett-Neto; title: Suppression subtractive hybridization as a tool to identify anthocyanin metabolism-related genes in apple skin / Yusuke Ban and Takaya Moriguchi; title: Identification of regulatory protein genes involved in alkaloid biosynthesis using a transient RNAi system / Yasuyuki Yamada ... [et al.]; title: Site-directed mutagenesis and saturation mutagenesis for the functional study of transcription factors involved in plant secondary metabolite biosynthesis / Sitakanta Pattanaik ... [et al.]; title: Isolation of proteins binding to promoter elements of alkaloid metabolism-related genes using yeast one-hybrid / Débora Vom Endt and Giancarlo Pasquali; title: Modulation of carotenoid accumulation in transgenic potato by inducing chromoplast formation with enhanced sink strength / Joyce Van Eck ... [et al.]; title: Over-expression of rate-limiting enzymes to improve alkaloid productivity / Tomoya Takemura ... [et al.]; title: Microbial expression of alkaloid biosynthetic enzymes for characterization of their properties / Hiromichi Minami, Nobuhiro Ikezawa, and Fumihiko Sato; title: Producing a recombinant flavin-containing monooxygenase from Coffea arabica in Escherichia coli for screening of potential natural substrates / Igor Cesarino and Paulo Mazzafera; title: Efficient production of active form recombinant cassava hydroxynitrile lyase using Escherichia coli in low-temperature culture / Hisashi Semba ... [et al.]; title: Introduction of the early pathway to taxol biosynthesis in yeast by means of biosynthetic gene cluster construction using SOE-PCR and homologous recombination / Pia Dahm and Stefan Jennewein; title: Biocatalytic synthesis of tritium (³H)-labelled taxa-4(5), 11(12)-diene, the pathway committing precursor of the taxoid diterpenoids / Hans Schmeer and Stefan Jennewein; title: USER cloning and USER fusion : the ideal cloning techniques for small and big laboratories / Hussam H. Nour-Eldin, Fernando Geu-Flores, and Barbara A. Halkier; title: Enrichment of carotenoids in flaxseed by introducing a bacterial phytoene synthase gene / Masaki Fujisawa and Norihiko Misawa; title: Metabolic engineering by plastid transformation as a strategy to modulate isoprenoid yield in plants / Tomohisa Hasunuma, Akihiko Kondo, and Chikahiro Miyake; title: Engineering high yields of secondary metabolites in Rubia cell cultures through transformation with rol genes / Victor P. Bulgakov, yuri N. Shkryl, and Galina N. Veremeichik; title: Flow cytometric methods to investigate culture heterogeneities for plant metabolic engineering / Vishal Gaurav, Martin E. Kolewe, and Susan C. Roberts; title: Phenylpropanoid biosynthesis in leaves and glandular trichomes of basil (Ocimum basilicum L.) / Cícero Deschamps and James E. Simon; title: Fusion with fluorescent proteins for subcellular localization of enzymes involved in plant alkaloid biosynthesis / Patrícia Duarte, Johan Memelink, and Mariana Sottomayor; title: Immunohistochemical localisation of a putative flavonoid transporter in grape berries / Elisa Petrussa ... [et al.]; title: Electrogenic bromosulfalein transport in isolated membrane vesicles : implementation of both animal and plant preparations for the study of flavonoid transporters / Sabina Passamonti ... [et al.]