High throughput protein expression and purification
Subtitle: methods and protocols
By: edited by Sharon A. Doyle.
Contributors: Doyle, Sharon A.
Series: Methods in molecular biology -- 498; Methods in molecular biology (Clifton, N.J.) -- 498.
Publisher: Humana Press; Springer, distributor]
Published: 2009
Place of publication: Totowa, N.J; [London
Language: eng
Number of pages: 322
ISBN-13: 9781588298799; 9781597451963
Subjects: Proteins -- Separation -- Methodology; Proteins -- genetics; Proteins -- isolation & purification; Gene Expression; Proteomics -- methods
Notes: Includes bibliographical references and index.
Table of contents: title: High-throughput protein production (HTTP) : a review of enabling technologies to expedite protein production / Jim Koehn and Ian Hunt; title: Designing experiments for high-throughput protein expression / Stephen P. Chambers and Susanne E. Swalley; title: Gateway cloning for protein expression / Dominic Esposito, Leslie A. Garvey, and Chacko S. Chakiath; title: Flexi vector cloning / Paul G. Blommel ... [et al.]; title: The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning / Nick S. Berrow, David Alderton, and Raymond J. Owens; title: The polymerase incomplete primer extension (PIPE) method applied to high-throughput cloning and site-directed mutagenesis / Heath E. Klock and Scott A. Lesley; title: A family of LIC vectors for high-throughput cloning and purification of proteins / William H. Eschenfeldt ... [et al.]; title: "System 48" high-throughput cloning and protein expression analysis / James M. Abdullah, Andrzej Joachimiak, and Frank R. Collart; title: Automated 96-well purification of hexahistidine-tagged recombinant proteins on MagneHis Ni²⁺-particles / Chiann-Tso Lin, Priscilla A. Moore, and Vladimir Kery; title: E. coli and insect cell expression, automated purification and quantitative analysis / StephenP. Chambers ... [et al.].; title: Hexahistidine-tagged maltose-binding protein as a fusion partner for the production of soluble recombinant proteins in Escherichia coli / Brian P. Austin, Sreedevi Nallamsetty, and David S. Waugh; title: PHB-intein-mediated protein purification strategy / Alison R. Gillies, Mahmoud Reza Banki, and David W. Wood; title: High-throughput biotinylation of proteins / Brian K. Kay, Sang Thai, and Veronica V. Volgina; title: High-throughput insect cell protein expression applications / Mirjam Buchs ... [et al.]; title: High-throughput protein expression using cell-free system / Kalavathy Sitaraman and Deb K. Chatterjee; title: The production of glycoproteins by transient expression in mammalian cells / Joanne E. Nettleship, Nahid Rahman-Huq, and Raymond J. Owens; title: High-throughput expression and detergent screening of integral membrane proteins / Said Eshaghi; title: Cell-free expression for nanolipoprotein particles : building a high-throughput membrane protein solubility platform / Jenny A. Cappuccio ... [et al.]; title: Expression and purification of soluble His₆-tagged TEV protease / Joseph E. Tropea, Scott Cherry, and David S. Waugh; title: High-throughput protein concentration and buffer exchange : comparison of ultrafiltration and ammonium sulfate precipitation / Priscilla A. Moore and Vladimir Kery.