Role of VEGF Signaling in Brown Adipocyte Survival, Proliferation and Function
Contributors: D'Amore, Patricia A.; Kahn, C Ronald; Arany, Zoltan; Dvorak, Harold; Bielenberg, Diane; Farmer, Stephen
Published: 2013
Language: eng
Description: Both white and brown adipose tissues exhibit extensive vascularity. Increased angiogenesis in brown adipose tissue (BAT) is crucial for brown fat activation and thermogenesis in animals during cold acclimation. BAT can be similarly activated by food intake to generate heat through cellular respiration, in a process known as diet induced thermogenesis. Vascular endothelial growth factor (VEGF) is a potent angiogenic factor that regulates both pathological and physiological angiogenesis and can stimulate cell proliferation, migration, survival and vessel permeability. However, VEGF has also been shown to affect an increasing number of non-vascular cells such as skeletal muscle and kidney podocytes. The expression and function of VEGF in white and brown adipocytes are not fully understood. We have previously shown that the expression of VEGF is concomitantly regulated with skeletal muscle differentiation. Here we show that VEGF is expressed in BAT and all major white adipose depots in mice. VEGF expression was increased during white and brown adipocyte differentiation and was regulated in cultured brown adipocytes by the PPARã agonist troglitazone and by PGC1á in BAT in vivo.
Notes: Keywords: apoptosis, brown fat, mitochondria, thermogenesis, Vascular Endothelial Growth Factor.

Thesis Ph.D. Harvard University 2013