Lipid-protein Interactions
Subtitle: methods and protocols
By: edited by Jörg H. Kleinschmidt, Institut für Biologie, Abteilung Biophysik, Universität Kassel, Kassel, Germany
Series: Methods in molecular biology -- v. 974; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 974.; Springer protocols (Series)
Publisher: Humana Press; Springer
Published: 2013
Place of publication: New York
Language: eng
Number of pages: 464
ISBN-13: 9781627032742; 9781627032759
Copyright date: 2013
Subjects: Lipids; Lipid membranes; Proteins; Membrane Proteins; Protein Binding
Description: Biological membranes are the essential structuring elements of all living cells. Many enzymatic reactions take place at the membrane-water interface. To gain detailed insight into membrane properties, it is therefore of great importance to understand the complex nature of the interactions of membrane proteins with lipids. Lipid-Protein Interactions: Methods and Protocols provides a selection of protocols to examine protein-lipid interactions, membrane and membrane protein structure, how membrane proteins affect lipids and how they are in turn affected by the lipid bilayer and lipid properties. The methods described here are all actively used, complementary, and necessary to obtain comprehensive information about membrane structure and function. They include label-free approaches, imaging techniques and spectroscopic methodologies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Lipid-Protein Interactions: Methods and Protocols seeks to serve both professional and novices with its wide range of the methods frequently used in this area of research.--
Notes: Includes bibliographical references and index.
Table of contents: title: Quartz crystal microbalances as tools for probing protein-membrane interactions  -- Søren B. Nielsen and Daniel E. Otzen; title: Surface plasmon resonance for measuring interactions of proteins with lipid membranes  -- Vesna Hodnik and Gregor Anderluh; title: Probing the thermodynamics of protein-lipid interactions by isothermal titration calorimetry  -- Musti J. Swamy and Rajeshwer S. Sankhala; title: Differential scanning calorimetry of protein-lipid interactions  -- Olga Canadas and Cristina Casals; title: Atomic force microscopy and spectroscopy to probe single membrane proteins in lipid bilayers  -- K. Tanuj Sapra; title: Optimized negative-staining protocol for examining lipid-protein interactions by electron microscopy  -- Mark Garewal, Lei Zhang, and Gang Ren; title: Examining protein-lipid complexes using neutron scattering  -- Luke A. Clifton, Cameron Neylon, and Jeremy H. Lakey; title: Circular-dichroism and synchrotron-radiation circular-dichroism spectroscopy as tools to monitor protein structure in a lipid environment  -- Koichi Matsuo and Kunihiko Gekko; title: Structural characterization of membrane proteins and peptides by FTIR and ATR-FTIR spectroscopy  -- Suren A. Tatulian; title: Forster resonance energy transfer as a tool for quantification of protein-lipid selectivity  -- Luıs M.S. Loura, Manuel Prieto, and Fabio Fernandes; title: Guide to tracking single transmembrane proteins in supported lipid bilayers  -- Kumud Raj Poudel, Jeffrey P. Jones, and James A. Brozik; title: Fluorescence correlation spectroscopy to examine protein-lipid interactions in membranes  -- Viktoria Betaneli and Petra Schwille; title: Analyzing transmembrane protein and hydrophobic helix topography by dual fluorescence quenching  -- Gregory A. Caputo and Erwin London; title: Studying lipid-protein interactions with electron paramagnetic resonance spectroscopy of spin-labeled lipids  -- Tibor Pa li and Zoltan Kota; title: EPR techniques to probe insertion and conformation of spin-labeled proteins in lipid bilayers  -- Enrica Bordignon and Yevhen Polyhach; title: Solid-state NMR approaches to study protein structure and protein-lipid interactions  -- Christopher Aisenbrey [and others]; title: Solution NMR spectroscopy for the determination of structures of membrane proteins in a lipid environment  -- Ashish Arora; title: Nanodiscs as a New Tool to Examine Lipid-Protein Interactions  -- Mary A. Schuler, Ilia G. Denisov, and Stephen G. Sligar; title: Simulation approach to lipid-protein interactions -- Teresa Paramo [and others].