Plasmodesmata
Subtitle: methods and protocols
By: edited by Manfred Heinlein, Institut de Biologie Moléculaire des Plantes (IBMP-CNRS), Strasbourg, France
Series: Methods in molecular biology -- 1217; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 1217.; Springer protocols (Series)
Publisher: Humana Press
Published: 2015
Place of publication: New York
Language: eng
Number of pages: 346
ISBN-13: 9781493915224; 9781493915231
Copyright date: 2015
Subjects: Laboratory manuals; Plasmodesmata; Laboratory Manuals; Plant Viruses; Pathogenicity
Notes: "Additional material to this book can be downloaded from http://extras.springer.com" -- Title page verso.

Includes  bibliographic references and index.
Table of contents: title: Plasmodesmata : channels for intercellular signaling during plant growth and development  -- Iris Sevilem, Shri Ram Yadav, and Ykä Helariutta; title: Plasmodesmata : channels for viruses on the move  -- Manfred Heinlein; title: Imaging plasmodesmata with high-resolution scanning electron microscopy  -- Deborah A. Barton and Robyn L. Overall; title: Preparative methods for imaging plasmodesmata at super-resolution  -- Karen Bell and Karl Oparka; title: Isolation of plasmodesmata from Arabidopsis suspension culture cells  -- Magali S. Grison, Lourdes Fernandez-Calvino, Sébastien Mongrand, and Emmanuelle M.F. Bayer; title: Immunofluorescence detection of callose deposition around plasmodesmata sites  -- Ali Pendle and Yoselin Benitez-Alfonso; title: Imaging callose at plasmodesmata using aniline blue : quantitative confocal microscopy  -- Raul Zavaliev and Bernard L. Epel; title: Localization of fluorescently tagged protein to plasmodesmata by correlative light and electron microscopy  -- Shannon Modla, Jeffrey L. Caplan, Kirk J. Czymmek, and Jung-Youn Lee; title: Quantification of plant cell coupling with live-cell microscopy  -- Johannes Liesche and Alexander Schulz; title: Drop-ANd-See : a simple, real-time, and noninvasive technique for assaying plasmodesmal permeability  -- Weier Cui, Xu Wang, and Jung-Youn Lee; title: Mapping symplasmic fields at the shoot apical meristem using iontophoresis and membrane potential measurements  -- Christiaan van der Schoot and Päivi L. H. Rinne; title: Analysis of the conductivity of plasmodesmata by microinjection  -- Friedrich Kragler; title: Investigating plasmodesmata genetics with virus-induced gene silencing and an agrobacterium-mediated GFP movement assay  -- Jacob O. Brunkard, Tessa M. Burch-Smith, Anne M. Runkel, and Patricia Zambryski; title: Probing plasmodesmata function with biochemical inhibitors  -- Rosemary G. White; title: GAL4 transactivation-based assay for the detection of selective intercellular protein Movement  -- Dhinesh Kumar, Huan Chen, Yeonggil Rim, and Jae-Yean Kim; title: Techniques for assessing the effects of pharmacological inhibitors on intercellular protein movement  -- Shuang Wu and Kimberly L. Gallagher; title: Probing protein targeting to plasmodesmata using fluorescence recovery after photo-bleaching  -- Kathryn M. Wright and Katrin M. MacKenzie; title: Tracking of intercellular small RNA movement  -- Christophe Himber and Patrice Dunoyer; title: Analysis of the role of myosins in targeting proteins to plasmodesmata  -- Martin Di Donato and Khalid Amari; title: Pumilio-based RNA in vivo imaging  -- Jens Tilsner; title: In vivo RNA labeling using MS2 -- Eduardo Peña, Manfred Heinlein, and Adrian Sambade.