Transcriptional regulation
Subtitle: methods and protocols
By: edited by Ales Vancura
Series: Springer protocols; Methods in molecular biology -- 809; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- 809.
Publisher: Humana Press
Published: 2012
Place of publication: New York
Language: eng
Number of pages: 627
ISBN-13: 9781617793752; 9781617793769
Subjects: Transcriptional Activation; Genetics; Genetic transcription; Gene Expression Regulation; Genetic Transcription; Regulation; Transcription Factors; Laboratory Manuals
Notes: Includes bibliographical references and index.
Table of contents: title: Genome-wide in vivo cross-linking of sequence-specific transcription factors / Xiao-Yong li and Mark D. Biggin; title: Characterization of complex regulatory networks and identification of promoter regulatory elements in yeast : "in silico" And "wet-lab" approaches / Nuno P. Mira, Miguel C. Teixeira, and Isabel Sá-Correia; title: Electrophoretic mobility shift assay analysis of NF[kappa]B transcriptional regulation by nuclear I[kappa]B[alpha] / Ashish Juvekar ... [et al.]; title: Probing endogenous RNA polymerase II pre-initiation complexes by electrophoretic mobility shift assay / Emmanuelle Wilhelm, Christopher Takacs, and Brendan Bell; title: Elucidating protein : DNA complex by oligonucleotide DNA affinity purification / Teddy T.C. Yang and Chi-Wing Chow; title: Chromatin immunoprecipitation assay as a tool for analyzing transcription factor activity / Padmaja Gade and Dhan V. Kalvakolanu; title: Two-step cross-linking for analysis of protein-chromatin interactions / Bing Tian, Jun Yan, and Allan R. Brasier; title: Chromatin immunoprecipitation analysis of NF[kappa]B transcriptional regulation by nuclear I[kappa]B[alpha] in human macrophages / Sitharam Ramaswami ... [et al.]; title: In vivo ChIP for the analysis of microdissected tissue samples / Chris Murgatroyd, Anke Hoffmann, and Dietmar Spengler; title: Quantification of protein-DNA interactions by in vivo chromatin immunoprecipitation in yeast / Amparo Pascual-Ahuir and Markus Proft; title: Mapping protein-DNA interactions using ChIP-sequencing / Charles E. Massie and Ian G. Mils; title: ChIP and re-ChIP assays : investigating interactions between regulatory proteins, histone modifications, and the DNA sequences to which they bind / Agnieszka D. Truax and Susanna F. Greer; title: Transcriptional regulation of genes via hypoxia-inducible factor / Olga Roche and Michael Ohh; title: Exchange protein directly activated by cyclic AMP-1-regulated recruitment of CCAAT/enhancer-binding proteins to the suppressor of cytokine signaling-3 promoter / William A. Sands ... [et al.]; title: Computational analysis of promoter elements and chromatin features in yeast / John J. Wyrick; title: Chromatin affinity purification / Ryoko Harada and Alain Nepveu; title: Determination of histone acetylation status by chromatin immunoprecipitation / Luciano Galdieri, John Moon, and Ales Vancura; title: Immunostaining of Drosophila polytene chromosomes to investigate recruitment of chromatin-binding proteins / Magdalena Murawska and Alexander Brehm; title: Detection of transcriptional activators, co-activators, and chromatin remodeling by chromatin immunoprecipitation coupled with real-time PCR / Tamara Y. Erkina and Alexandre M. Erkine; title: Chromatin endogenous cleavage and psoralen crosslinking assays to analyze rRNA gene chromatin in vivo / Joachim Griesenbeck ... [et al.]; title: UV-induced DNA damage and DNA repair in ribosomal genes chromatin / Julie Pelloux ... [et al.]; title: Analysis of SUC2 promoter structure by nucleosome scanning / Jennifer Chang and Ales Vancura; title: Chromatin immunoprecipitation of mouse embryos / Anne K. Voss ... [et al.]; title: Chromatin immunoprecipitation in mouse hippocampal cells and tissues / Badi Sri Sailaja, Takumi Takizawa, and Eran Meshorer; title: Approaches for studying nucleosome movement by ATP-dependent chromatin remodeling complexes / Swetansu K. Hota and Blaine Bartholomew; title: Mapping protein-DNA and protein-protein interactions of ATP-dependent chromatin remodelers / Swetansu K. Hota ... [et al.]; title: Evaluation of histone-modifying enzymes in stem cell populations / Leanne Stalker and Christopher Wynder; title: Purification of multiprotein histone acetyltransferase complexes / Yuan-Liang Wang, Francesco Faiola, and Ernest Martinez; title: Reconstitution of active and stoichiometric multisubunit lysine acetyltransferase complexes in insect cells / Kezhi Yan ... [et al.]; title: Affinity purification of MLL3/MLL4 histone H3K4 methyltransferase complex / Young-Wook Cho, SunHwa Hong, and Kai Ge; title: Methods for analyzing histone citrullination in chromatin structure and gene regulation / Pingxin Li, Jing Hu, and Yanming Wang; title: Analysis of mRNA abundance and stability by ribonuclease protection assay / Cristina Romero-López ... [et al.]; title: Array-based nuclear run-on analysis / Jinshui Fan ... [et al.]; title: In vivo run-on assays to monitor nascent precursor RNA transcripts / Piergiorgio Percipalle and Emilie Louvet; title: Genome wide full-length transcript analysis using 5' and 3' paired-end-tag next generation sequencing (RNA-PET) / Xiaoan Ruan and Yijun Ruan; title: Analysis of co-transcriptional RNA processing by RNA- ChIP assay / Danielle Bittencourt and Didier Auboeuf; title: Quantitative analysis of transcription elongation by RNA polymerase I in vitro / David Alan Schneider; title: Detection and characterization of transcription termination / Ghada Ghazal, Jules Gagnon, and Sherif Abou Elela; title: Promoter-associated noncoding RNA from the CCND1 promoter / Xiaoyuan Song ... [et al.]