Nucleic acid and peptide aptamers
Subtitle: methods and protocols
By: edited by Günter Mayer.
Contributors: Mayer, Günter, 1972-
Series: Methods in molecular biology -- 535; Springer protocols
Publisher: Humana
Published: 2009
Place of publication: New York, NY
Language: eng
Number of pages: 408
ISBN-13: 9781934115893; 9781597455572
Subjects: Aptamers, Nucleotide; Aptamers, Peptide; Oligonucleotides; Nucleotide sequence; Amino acid sequence
Description: "After the deciphering of the human genome and the genomes of many other organisms, the investigation of the function of gene products and their orchestral interplay is now one of the most important challenges in the life sciences. In Nucleic Acid and Peptide Aptamers: Methods and Protocols, expert researchers contribute state-of-the-art methods focused on these two vital molecule types which are so often employed for in vitro selection procedures. Divided conveniently into two distinct parts beginning with nucleic acid aptamers and ending with peptide aptamers, the volume provides methodologies for the isolation, characterization, and application of both types. Written in the highly successful Methods in Molecular Biology series format, all chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known

Pitfalls." "Cutting-edge and easy to use, Nucleic Acid and Peptide Aptamers: Methods and Protocols will provide researchers with an inspiring and helpful guide to the application of these compounds to their own distinct research issues."--Jacket
Notes: Includes bibliographical references and index.
Table of contents: title: Nucleic acid pool preparation and characterization / Shawn K. Piasecki, Bradley Hall, and Andrew D. Ellington; title: In vitro selection of ssDNA aptamers using biotinylated target proteins / Günter  Mayer and Thomas Höver; title: Isolating aptamers using capillary electrophoresis-SELEX (CE-SELEX) / Renee K. Mosing and Michael T. Bowser; title: In vitro selection of allosteric ribozymes / Nicolas Piganeau; title: Cell-specific aptamers for targeted therapies / Laura Cerchia ... [et al.]; title: Aptamers targeting RNA molecules / Marguerite Watrin ... [et al.]; title: Fluorescence correlation spectroscopy (FCS)-based characterisation of aptamer ligand interaction / Arne Werner and Ulrich Hahn; title: Structural probing techniques on natural aptamers / Catherine A. Wakeman and Wade C. Winkler; title: Determining structures of RNA aptamers and riboswitches by X-ray crystallography / Andrea L. Edwards, Andrew D. Garst, and Robert T. Batey; title: Locked nucleic acid aptamers / Jan Barciszewski ... [et al.]; title: Screening of novel inhibitors of HIV-1 reverse transcriptase with a reporter ribozyme assay / Satoko Yamazaki and Michael Famulok; title: Aptamers as artificial gene regulation elements / Beatrix Suess and Julia E. Weigand; title: Aptamers and biosensors / Thomas M. Gronewold; title: Nanoparticles/dip stick / Yi Lu, Juewen. Liu, and Debapriya  Mazumdar; title: In vivo imaging of oligonucleotidic aptamers / Bertrand Tavitian ... [et al.]; title: Selection of peptides interfering with protein-protein interaction / Annette Gaida ... [et al.]; title: In vitro selection of protein and peptide libraries using mRNA display / Terry T. Takahashi and Richard W. Roberts; title: Ligand-regulated peptide aptamers / Russell A. Miller; title: Isolation of peptide aptamers to target protein function / Luisa Lopez-Ochoa ... [et al.]; title: Microbodies / Hans-Ulrich. Schmoldt ... [et al.]; title: Peptide aptamers for small molecule drug discovery / Carine Bardou ... [et al.]; title: Synthesis and application of peptides as drug carriers / Robert Rennert, Ines Neundorf, and Annette G. Beck-Sickinger.