Molecular mechanisms of Fas mediated cell death in oligodendrocytes.
Published: 2006
Language: eng
Number of pages: 92
ISBN-13: 9780494213087
Description: Hypothesis. FasR activation in oligodendrocytes results in apoptosis, which is linked to extrinsic and intrinsic signaling pathways.Background. Apoptotic cell death contributes significantly to the secondary injury following spinal cord injury (SCI). Moreover, the Fas receptor (FasR) has been implicated to contribute to this delayed cellular loss, particularly in oligodendrocytes.Discussion. Fas activation resulted in apoptosis mediated through intrinsic and possible extrinsic events. Additionally, AIF has been implicated to be a caspase-independent executioner involved in FasR signaling.Methods. Soluble Fas ligand (sFasL) was applied to cycloheximide sensitized human oligodendroglial cells (MO3.13 cell fine). Experiments assessed caspase activation, mitochondrial dysfunction, and cell death.Results. sFasL treatment resulted in caspase-8, -9, and -3 activation. Loss of mitochondrial membrane potential (DeltaPsim) temporally coincided with caspase-9 activation and mitochondrial release of cytochrome c and AIF. Apoptotic cell death was determined using propidium iodide and was confirmed with deoxynucleotide transferase dUTP nick end-labeling (TUNEL).
Notes: Source: Masters Abstracts International, Volume: 45-03, page: 1382.

Thesis (M.Sc.)--University of Toronto, 2006.

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