Basophils and mast cells
Subtitle: methods and protocols
By: edited by Bernhard F. Gibbs, Medway School of Pharmacy, The University of Kent, Chatham Maritime, Kent, UK, Franco H. Falcone, Division of Molecular and Cellular Science, The School of Pharmacy, The University of Nottingham, Nottingham, UK
Series: Methods in molecular biology -- 1192; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 1192.; Springer protocols (Series)
Publisher: Humana Press
Published: 2014
Place of publication: New York
Language: eng
Number of pages: 251
ISBN-13: 9781493911721; 9781493911738
Subjects: Immunology; Basophils; Laboratory manuals; Mast cells; Immunomodulation; Laboratory Manuals; Mast Cells
Description: "In Basophils and Mast Cells: Methods and Protocols, experts in this challenging field explore techniques to research these cells from the most practical point of view. Given the tremendous influence of mast cells and blood-borne basophils over immune system function, this volume intends to aid the reader in the development of better tools for the isolation of these cells from primary tissues, peripheral blood, bone marrow, or cord blood. Also covered are protocols for the in vitro and in vivo study of their functions."--Publisher's description.
Notes: Includes bibliographical references and index.
Table of contents: title: Paradigm shifts in mast cell and basophil biology and function : an emerging view of immune regulation in health and disease  -- Ana Olivera and Juan Rivera; title: Purification of basophils from peripheral human blood  -- Franco H. Falcone and Bernhard F. Gibbs; title: Mast cell purification protocols  -- Jasmine Farrington , Elizabeth P. Seward , and Peter T. Peachell; title: Generation of a human allergic mast cell phenotype from CD133+ stem cells  -- Hans Jürgen Hoffmann; title: Generation of mast cells from murine stem cell progenitors  -- Emily J. Swindle; title: Integration of the human dermal mast cell into the organotypic co-culture skin model  -- Jonghui Kim [and four others]; title: The absolute basophil count  -- Elena Borzova and Clemens A. Dahinden; title: Mast cell and basophil cell lines : a compendium -- Egle Passante.; title: Detection of mast cells and basophils by immunohistochemistry  -- Andrew F. Walls and Cornelia Amalinei; title: Measuring histamine and cytokine release from basophils and mast cells  -- Bettina M. Jensen, Sidsel Falkencrone, and Per S. Skov; title: Flow cytometric allergy diagnosis : basophil activation techniques  -- Chris H. Bridts [and five others]; title: Microscopy assays for evaluation of mast cell migration and chemotaxis  -- Monika Bambousková [and three others]; title: Use of humanized Rat Basophil Leukemia (RBL) reporter systems for detection of allergen-specific IgE sensitization in human serum  -- Daniel Wan [and four others]; title: Gene silencing approaches in mast cells and primary human basophils  -- Vadim V. Sumbayev and Bernhard F. Gibbs; title: Basophil stimulation and signaling pathways  -- Edward F. Knol and Bernhard F. Gibbs; title: Identification and immunophenotypic characterization of normal and pathological mast cells  -- José Mário Morgado [and three others]; title: Identification of murine basophils by flow cytometry and histology  -- Christian Schwartz and David Voehringer; title: Mast cell-mediated reactions in vivo -- Vladimir Andrey Giménez-Rivera , Martin Metz, and Frank Siebenhaar.