Characterizing anti-tumor activity mediated by double negative T cells.
Published: 2006
Language: eng
Number of pages: 100
ISBN-13: 9780494161517
Description: Recently our lab has demonstrated that infusion of allogeneic alphabetaTCR +CD3+CD4-CD8-NK1.1 - double negative (DN) T cell clones can eliminate tumor cells without causing graft versus host disease. Here we investigated whether primary DN T cells can prevent tumor progression and the mechanisms involved. We demonstrated that injection of single MHC-mismatched primary DN T cells can prevent tumor (A20) progression. Although DN T cells produced high amounts of IFNgamma, their cytotoxicity to A20 tumor is IFNgamma independent and contact dependent  in vitro. Blocking the interactions between the T cell receptors (TCR) on DN T cells and alloantigen on tumors impaired their cytotoxicity, whereas transducing a single MHC class I alloantigen on syngeneic tumor cells is sufficient for DN T cells to recognize and kill tumor cells. These findings enhance our understanding of the anti-tumor activity of DN T cells, and suggesting DN T cells as a potential candidate for cancer immunotherapy.
Notes: Source: Masters Abstracts International, Volume: 44-06, page: 2757.

Thesis (M.Sc.)--University of Toronto, 2006.

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