RNAi and microRNA-mediated gene regulation in stem cells
Subtitle: methods, protocols, and applications
By: edited by Baohong Zhang and Edmund J. Stellwag
Series: Methods in molecular biology -- 650; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 650.; Springer protocols
Publisher: Humana Press
Published: 2010
Place of publication: New York
Language: eng
Number of pages: 261
ISBN-13: 9781607617686; 9781607617693
Subjects: RNA Interference; Stem Cells; RNA; Analysis; Gene Expression Regulation; Genetic regulation; Stem cells; Laboratory Manuals; MicroRNAs
Notes: Includes bibliographical references and index.
Table of contents: title: RNAi in stem cells : current status and future perspectives / Gang-Ming Zou; title: A brief introduction to RNAi and microRNAs in stem cells / Alexander K. Murashov; title: Forward RNAi screens in human stem cells / Christine Karlsson, Jonas Larsson, and Aurélie Baudet; title: A recessive genetic screen for components of the RNA interference pathway in mouse embryonic stem cells / Melanie I Trombly and Xiaozhong Wang; title: Construction and application of random dsRNA interference library for functional genetic screens in embryonic stem cells / Xiaoxing Cheng and Rui Jian; title: Establishing efficient siRNA knockdown in stem cells using fluorescent oligonucleotides / Stephen W. Chen and Steve K.W. Oh; title: Loss-of-function studies in mouse embryonic stem cells using the pHYPER shRNA plasmic vector / Soizik Berlivet, Martin Houlard, and Matthieu Gérard; title: Regulation and/or repression of cholinergic differentiation of murine embryonic stem cells using RNAi directed against transcription factor L3/Lhx8 / Takayuki Manabe and Akio Wanaka; title: Silencing of rho-GDI[gamma] by RNAi promotes the differentiation of neural stem cells / Jiao Wang, Wei Lu, and Tieqiao Wen; title: RNAi knockdown of redox signaling protein Ape1 in the differentiation of mouse embryonic stem cells / Gang-Ming Zou, Cynthia LeBron, and Yumei Fu; title: Proteins involved in cell migration from glioblastoma neurospheres analyzed by overexpression and siRNA-mediated knock-down / Carsten Hagemann ... [et al.]; title: An efficient transfection method for mouse embryonic stem cells / Jun-Yang Liou, Bor-Sheng Ko, and Tzu-Ching Chang; title: Semi-quantitative analysis of transient single-cell gene expression in embryonic stem cells by femtoinjection / Mikako Saito and Hideaki Matsuoka; title: Preparation and analysis of microRNA libraries using the Illumiina massively parallel sequencing technology / Ryan D. Morin ... [et al.]; title: Assessing in vivo microRNA function in the germline stem cells of the Drosophila ovary / Kin Chan and Hannele Ruohola-Baker; title: Monitoring microRNA expression during embryonic stem-cell differentiation using quantitative real-time PCR (qRT-PCR) / Xiaoping Pan ... [et al.]; title: Engineering human mesenchymal stem cells to release adenosine using miRNA technology / Gaoying Ren and Detlev Boison; title: Efficient gene knockdowns in mouse embryonic stem cells using microRNA-based shRNAs / Jianlong Wang.