Cytochrome P450 protocols
By: edited by Ian R. Phillips, Elizabeth A. Shephard, Paul R. Ortiz de Montellano
Edition: 3rd ed.
Series: Methods in molecular biology -- 987; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 987.; Springer protocols (Series)
Publisher: Humana
Published: 2013
Place of publication: New York
Language: eng
Number of pages: 319
ISBN-13: 9781627033206; 9781627033213
Subjects: Cytochrome P-450; Laboratory manuals; Cytochrome P-450 Enzyme System
Notes: Includes bibliographical references and index.
Table of contents: title: Bioluminescent assays for cytochrome P450 enzymes / Douglas S. Auld, Henrike Veith, and James J. Cali; title: Simultaneous determination of multiple CYP inhibition constants using a cocktail-probe approach / Michael Zientek and Kuresh Youdim; title: High-throughput mass spectrometric cytochrome  P450 inhibition screening / Kheng B. Lim, Can C. Ozbal, and Daniel B. Kassel; title: Synthesis, characterization, and application of ¹³c-methyl isocyanide as an NMR probe of heme protein active sites / Christopher McCullough ... [et al.]; title: High-throughput fluorescence assay for cytochrome P450 mechanism-based Inactivators / Cesar Kenaan, Haoming Zhang, and Paul F. Hollenberg; title: Identification of endogenous substrates of orphan cytochrome P450 enzymes through the use of untargeted metabolomics approaches / Qian Cheng and F. Peter Guengerich; title: Genetic and mass spectrometric tools for elucidating  the physiological function(s) of cytochrome P450 enzymes  from Mycobacterium tuberculosis / Hugues Ouellet ... [et al.]; title: Escherichia coli expression-based approach for porphyrin substitution in heme proteins / Michael B. Winter, Joshua J. Woodward, and Michael A. Marletta; title: Expression in Escherichia coli of a cytochrome P450 enzyme with a cobalt protoporphyrin IX porosthetic group / Wesley E. Straub ... [et al.]; title: Nanodiscs in the studies of membrane-bound cytochrome  P450 enzymes / A. Luthra ... [et al.]; title: Rapid LC-MS drug metabolite profiling using bioreactor particles / Linlin Zhao, Besnik Bajrami, and James F. Rusling; title: Fluorescence-based screening of cytochrome P450 activities in intact cells / M. Teresa Donato and M. José Gómez-Lechón; title: Screening for cytochrome P450 reactivity with a reporter enzyme / Kersten S. Rabe and Christof M. Niemeyer; title: High-throughput fluorescence assay of cytochrome P450 3A4 / Qian Cheng and F. Peter Guengerich; title: Targeted protein capture for analysis of electrophile-protein adducts / Rebecca E. Connor ... [et al.]; title: DNA shuffling of cytochrome P450 enzymes / James B.Y.H. Behrendorff, Wayne A. Johnston,  and Elizabeth M.J. Gillam; title: Measurement of P450 difference spectra using intact cells / Wayne A. Johnston and Elizabeth M.J. Gillam; title: DNA shuffling of cytochromes P450 for indigoid pigment production / Nedeljka N. Rosic; title: P450 oxidoreductase : genotyping, expression, purification of recombinant  protein, and activity assessments of wild-type and mutant protein / Vishal Agrawal and Walter L. Miller; title: LICRED : a versatile drop-in vector for rapid generation of redox-self-sufficient cytochromes P450 / Federico Sabbadin, Gideon Grogan, and Neil C. Bruce; title: Update on allele nomenclature for human cytochromes P450 and the human cytochrome P450 allele (CYP-allele) nomenclature database / Sarah C. Sim and Magnus Ingelman-Sundberg; title: Simultaneous in vivo phenotyping of CYP enzymes / Sussan Ghassabian and Michael Murray; title: Detection of regulatory polymorphisms : high-throughput capillary  DNase I footprinting / Matthew Hancock and Elizabeth A. Shephard; title: Isolation of mouse hepatocytes / Mina Edwards ... [et al.]; title: Highly efficient SiRNA and gene transfer into hepatocyte-like  HepaRG cells and primary human hepatocytes : new means for drug metabolism and toxicity studies / Véronique Laurent ... [et al.].