Electroporation protocols
Subtitle: preclinical and clinical gene medicine
By: edited by Shulin Li, Department of Pediatrics, The University of Texas MD Anderson Cancer Center, Houston, TX, USA, Jeffry Cutrera, Department of Pediatrics, The University of Texas MD Anderson Cancer Center, Houston, TX, USA, Richard Heller, Old Dominion University, Frank Reidy Center for Biolectrics, Norfolk, VA, USA, Justin Teissie, Centre National de la Recherche Scientifique, Institut de Pharmacologie et de Biollogie structurale, Toulouse, France, Université de Toulouse, UPS, IPBS Toulouse, France
Edition: Second edition.
Series: Methods in molecular biology -- 1121; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 1121.; Springer protocols (Series)
Publisher: Humana Press
Published: 2014
Place of publication: New York
Language: eng
Number of pages: 349
ISBN-13: 9781461496311; 9781461496328
Copyright date: 2014
Subjects: Electroporation; Therapeutic use; Laboratory manuals; Gene therapy; Geneti Therapy; Laboratory Manuals; Electrochemotherapy
Description: Electroporation gene therapy, or gene electrotransfer, has evolved greatly over the last few decades as a result of the remarkable progress in genetic sequencing, gene array analysis, gene cloning, gene expression detection, DNA manufacture, and discovery and synthesis of siRNA. Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted cells, tissues, and animals for prevention and treatment of disease. It builds on the success of the first edition and on the progress made in siRNA delivery and DNA vaccines for large animals as well as discovery of electroporation applications for the fragile tissues, and for internal organs. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition aims to provide not only comprehensive coverage of the basic theory and practical application of electroporation siRNA therapy, gene therapy, and vaccine, but also elaborates on the most current views from the experts in this field, serving as an invaluable resource for investigators both in and outside of this field. --
Notes: Includes bibliographical references and index.
Table of contents: title: 1. Electroporation-based gene therapy; recent evolution in the mechanism description and technology developments / Lluis M. Mir; title: 2. Electropermeabilization of the cell membrane / Justin Teissie; title: 3. The impact of non-electrical factors on electrical gene transfer / Jiemino Hu, Jeffry Cutrera, and Shulin Li; title: 4. Electroporation formulation for cell therapy / Jiemiao Hu and Shulin Li; title: 5. Short-fragment DNA-mediated in vivo DNA electroporation delivery / Jinliang Peng, Yonggang Zhao and Yuhong Xu; title: 6. Gold nanoparticle-enhanced electroporation for leukemia cell transfection / Shuyan Huang, Yingbo Zu and Shengnian Wang; title: 7. siRNA delivery via electropulsation: a review of the basic processes / Muriel Golzio and Justin Teissie; title: 8. Continuous cell electroporation for efficient DNA and siRNA delivery based on laminar microfluidic chips / Zewen Wei and Zhihong Li; title: 9. Electroporation of siRNA into mouse bone marrow-derived macrophages and dendritic cells / Isabel Siegert, Valentin Schatz, Alexander T. Prechtel, Alexander Steinkasserer, Christian Bogdan and Jonathan Jantsch; title: 10. Directo imaging of siRNA electrotransfer at the single-cell level / Justin Teissie and Muriel Golzio; title: 11. Electroporation mediated siRNA delivery into tumors / Yashifumi Takei; title: 12. Substrate-mediated, high-efficiency siRNA electroporation / Hiroyuki Fujimato and Hiroo Iwata; title: 13. Intradermal electroporation / Maria L. Knudsen, Karl Ljungberg, Peter Liljeström and Daniel X. Johansson; title: 14. Delivery of DNA into the central nerous system via electroporation / Jochen De Vry, Tim Vanmierlo, Pilar Martínez-Martínez, Mario Losen, Yasin Temel, Harry Steinbusch, Marc De Baets and Jos Prickaerts; title: 15. DNA delivery in adult mouse eyes: an update with corneal outcomes / John M. Nickerson, Shannon E. Getz, Jana T. Sellers, Micah A. Chrenek, Penny Goodman, Christiana J. Bernal and Jeffrey H. Boatright; title: 16. Delivery of plasmid DNA into dental tissues of developing rat teeth by electroporation / Shaomian Yao, Michael L. Beckley and Dawen Liu; title: 17. Electroporation-mediated gene delivery to the lungs / Jennifer L. Young, Michael S. Barravecchia and David A. Dean; title: 18. Electroporation-mediated delivery of genes in rodent models of lung contusion / David Machado-Aranda and Krishnan Raghavendran; title: 19. In vivo electroporation-mediated gene delivery to the beating heart / Hendrik T. Tevaearai, Amiq Gazdhar, Marie-Noëlle Giraud and Martin Flück; title: 20. Managing local swelling following intratumoral electro-chemo-gene therapy / Jeffry Cutrera, Glenn King, Pamela Jones, Elias Gumpel, Xueqing Xia and Shulin Li; title: 21. Gene electrotransfer in clinical trials / Julie Gehl; title: 22. Electrochemotherapy in veterinary oncology: from rescue to first line therapy / Enrico P. Spugnini and Alfonso Baldi; title: 23. Low-energy DC current ablation in a mouse tumor model / Prejesh Philips, Yan Li and Robert C.G. Martin II; title: 24. Evolution of electroporated DNA vaccines / Andrea M. Keane-Myers and Matt Bell; title: 25. Clinical development of intramuscular electroporation: providing a "boost" for DNA vaccines / Amir S. Khan, Kate E. Broderick and Niranian Y. Sardesai; title: 26. Electroporation-mediated administration of candidate DNA vaccines against HIV -1 / Sandhya Vasan; title: 27. Electroporation-mediated intradermal delivery of DNA vaccines in nonhuman primates / Lucille Adam, Roger Le Grand and Fré́déric Martinon; title: 28. Intramuscular DNA vaccination protocols mediated by electric fields / Pieranna Chiarella and Emanuela Signori; title: 29. DNA electroporation of multi-agent vaccines conferring protection against select agent challenge: TriGrid delivery system / Andrea M. Keane-Myers, Matt Bell, Drew Hannaman and Mark Albrecht; title: 30. Gene-based vaccination and screening methods to develop monoclonal antibodies / M. Pirouz Daftarian, Aram Vosoughi and Vance Lemmon.