Stress induced modulation of mitochondrial protein import in differentiated PC12 cells: The role of Tom20.
Published: 2006
Language: eng
Number of pages: 141
ISBN-13: 9780494213797
Description: Although mitochondria contain their own DNA, 99% of mitochondrial proteins are nuclear-encoded and imported into mitochondria. Consequently even relatively minor defects in the import process can be expected to affect multiple mitochondrial functions. Mitochondrial protein import was not altered after a 5hr GD insult, but significantly decreased by 22 +/- 4% and 32% +/- 5% at 24 and 48hrs post-GD respectively. Mitochondrial membrane potential was not affected by the GD insult, but ROS generation was increased significantly at 24 and 48hrs post-GD. Western blots showed that by 24hrs post-GD, levels of Tom 20, an essential translocase of the import machinery, were significantly decreased. Transfection with Tom 20 significantly increased the import of mtGFP to mitochondria by 29 +/- 3% by 24hrs and 38 + 4% by 48hrs as assessed by flow cytometry (n=3). Over-expression of Tom20 prior to GD prevented the GD mediated decline in mtGFP/Tom20 expression and import.
Notes: Source: Masters Abstracts International, Volume: 45-03, page: 1389.

Thesis (M.Sc.)--University of Toronto, 2006.

Electronic version licensed for access by U. of T. users.

ROBARTS MICROTEXT copy on microfiche.