Drosophila
Subtitle: methods and protocols
By: edited by Christian Dahmann.
Contributors: Dahmann, Christian.
Series: Methods in molecular biology -- 420; Methods in molecular biology (Clifton, N.J.) -- 420.
Publisher: Humana Press
Published: 2008
Place of publication: Totowa, N.J
Language: eng
Number of pages: 437
ISBN-13: 9781588298171; 9781597455831
Subjects: Drosophila.; Molecular biology -- Research.; Molecular biology -- Technique.; Animal models in research.; Drosophila melanogaster -- Laboratory Manuals.; Models, Animal -- Laboratory Manuals.; Molecular Biology -- methods -- Laboratory Manuals.
Genres: Laboratory Manuals.
Notes: Includes bibliographical references and index.
Table of contents: title: Drosophila melanogaster and the development of biology in the 20th century / Alfonso Martinez Arias; title: Getting started : an overview on raising and handling Drosophila / Hugo Stocker and Peter Gallant; title: FlyBase : a database for the Drosophila research community / Rachel Drysdale and the FlyBase Consortium; title: The use of P-element transposons to generate transgenic flies / André Bachmann and Elisabeth Knust; title: The GAL4 system : a verstile system for the expression of genes / David A. Elliott and Andrea H. Brand; title: P-element mutagenesis / Thomas Hummel and Christian Klämbt; title: EMS screens : from mutagenesis to screening and mapping / Christian Bökel; title: RNAi screening in cultured Drosophila cells / Sandra Steinbrink and Michael Boutros; title: Methods for homologous recombination in Drosophila / Keith A. Maggert, Wei J. Gong, and Kent G. Golic; title: Recombinases and their use in gene activation, gene inactivation, and transgenesis / Johannes Bischof and Konrad Basler; title: Cuticle preparation of Drosophila embryos and larvae / Cyrille Alexandre; title: Immunolabeling of embryos / H.-Arno J. Müller; title: Imaging cellular and molecular dynamics in live embryos using fluorescent proteins / Matthieu Cavey and Thomas Lecuit; title: Analysis of neuromuscular junctions : histology and in vivo imaging / Andreas Schmid and Stephan J. Sigrist; title: Immunolabeling of imaginal discs / Thomas Klein; title: Imaging Drosophila pupal wing morphogenesis / Anne-Kathrin Classen ... [et al.]; title: Microscopic analysis of the adult Drosophila retina using semithin plastic section / Konstantin Gaengel and Marek Mlodzik; title: Fluorescent in situ hybridization protocols in Drosophila embryos and tissues / Eric Lécuyer, Neela Parthasarathy, and Henry M. Krause; title: Linear RNA amplification for the production of microarray hybridization probes / Ansgar Klebes and Thomas B. Kornberg; title: An overview of the identification, detection, and functional analysis of Drosophila microRNAs / Nicholas S. Sokol; title: Extraction and immunoblotting of proteins from embryos / Andreas Wodarz; title: Purification of Drosophila protein complexes for mass spectrometry / Christoph Jüschke and Jürgen A. Knoblich; title: Mass production of Drosophila embryos and chromatographic purification of native protein complexes / Natascha Kunert and Alexander Brehm; title: Flow cytometric analysis of Drosophila cells / Aida Flor A. de la Cruz and Bruce A. Edgar; title: Drosophila cell lines as model systems and as an experimental tool / Buzz Baum and Lucy Cherbas; title: Appendix : phenotypic markers in Drosophila / Jennifer Childress and Georg Halder.