Lipases.
By: edited by Byron Rubin, Edward A. Dennis.
Contributors: Rubin, Byron.; Dennis, Edward A.
Series: Methods in enzymology -- v. 286.
Publisher: Academic Press
Published: 1997
Place of publication: San Diego
Language: eng
Number of pages: 563
Subjects: Lipase.; Enzymes.; Lipase -- chemistry.; Lipase -- pharmacokinetics.; Lipase -- metabolism.
Notes: Includes bibliographical references and indexes.
Table of contents: title: Sect.1. Regulation and characterization; title: Inhibition of lipid absorption as an approach to the treatment of obesity; title: Regulation of hormone-sensitive lipase activity in adipose tissue; title: Use of gene knockout mice to establish lipase function; title: Properties of pancreatic carboxyl ester lipase in mRNA-injected Xenopus oocytes and transfected mammalian hepatic and intestinal cells; title: Noncatalytic functions of lipoprotein lipase; title: Radiation inactivation studies of hepatic cholesteryl ester hydrolases; title: Immunological techniques for the characterization of digestive lipases; title: Sect.2. Substrate and inhibitor characterization; title: Physical behavior of lipase substrates; title: Phospholipase A₂ activity and physical properties of lipid-bilayer substances; title: Covalent inactivation of lipases; title: Mechanism-based inhibitors of mammalian cholesterol esterase; title: On the inhibition of microbial lipases by tetrahydrolipstatin; title: Monolayer techniques for studying lipase kinetics; title: Recovery of monomolecular films in studies of lipolysis; title: Oil-drop tensiometer: applications for studying the kinetics of lipase action; title: A critical reevaluation of the phenomenon of interfacial activation; title: Sect.3. Biocatalytic utility; title: Screening techniques for lipase catalyst selection; title: Kinetics, molecular modeling, and synthetic applications with microbial lipases; title: Ester synthesis via acyl transfer (transesterification); title: Cross-linked enzyme crystals of lipases as catalysts for kinetic resolution of acids and alcohols; title: Measurement and control of hydration in non-aqueous biocatalysis; title: Solvent effect in lipase-catalyzed racemate resolution; title: Lipases in supercritical fluids; title: Stabilization of lipases against deactivation of acetaldehyde formed in acyl transfer reactions.