T follicular helper cells
Subtitle: methods and protocols
By: edited by Marion Espéli, INSERM UMR_S996, LabEx LERMIT, Université Paris-Sud, Clamart, France, Michelle Linterman, Lymphocyte Signalling and Development ISP, Babraham Institute, Babraham Research Campus, Cambridge, UK
Series: Methods in molecular biology -- 1291; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 1291.; Springer protocols (Series)
Publisher: Humana Press
Published: 2015
Place of publication: New York, NY
Language: eng
Number of pages: 228
ISBN-13: 9781493924974; 9781493924981
Copyright date: 2015
Subjects: Physiological effect; Laboratory manuals; Th2 cells; T-Lymphocytes, Helper-Inducer; Laboratory Manuals; T cells; Th1 cells
Description: This volume brings together the skills and protocols of numerous laboratories that are at the heart of investigation into the biology of Tfh cells in both mice and humans. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Concise and easy-to-use, T follicular Helper Cells: Methods and Protocols provides scientist with techniques and protocols that have facilitated breakthroughs in Tfh biology and to present them in a way that will enable both new and experienced researchers to continue to move this exciting field forward. --
Notes: Includes bibliographical references and index.
Table of contents: title: Identification of Mouse T Follicular Helper Cells by Flow Cytometry; title: Identification of Follicular T Helper Cells in Tissue Sections; title: Tracking Early T Follicular Helper Cell Differentiation In vivo; title: Tracking by Flow Cytometry Antigen-Specific Follicular Helper T-Cells In Wild-Type Animals After Protein Vaccination; title: Retroviral Vector Expression in TCR Transgenic CD4+ T Cells; title: Two-photon Microscopy For Imaging Germinal Centres and T Follicular Helper Cells; title: In vivo Induction of T-follicular Helper Cells by Modulation of Regulatory T Cell Function; title: Assessing T Follicular Helper Cell Function in vivo: Antigen-specific B Cell Response to Hapten and Affinity Maturation; title: The SWHEL System For High-resolution Analysis of in vivo Antigen-specific T-Dependent B Cell Responses; title: Triggering Positive Selection of Germinal Center B cells by Antigen Targeting to DEC-205; title: Detection of Mouse Natural Killer T Follicular Helper (NKTFH) Cells by Flow Cytometry; title: Identification of Foxp3+ T Follicular Regulatory (Tfr) Cells by Flow Cytometry; title: In vitro Assay to Sensitively Measure Tfr Suppressive Capacity and Tfh Stimulation of B Cell Responses; title: Flow Cytometric Detection and Isolation of Human Tonsil or Lymph Node T Follicular Helper Cells; title: Human Tfh and Tfr cells: Identification and Assessment of their Migration Potential; title: Analysis of Human Blood Memory T Follicular Helper Subsets; title: Flow Cytometric Analysis of Circulating Follicular Helper T (Tfh) and Follicular Regulatory T (Tfr) Populations in Human Blood; title: Quantifying Helper Cell Function of Human TFH Cells in vitro.