Effect of Src activation on NMDA receptors containing different NR2 subunits.
Published: 2007
Language: eng
Number of pages: 142
ISBN-13: 9780494274941
Description: Src is known to potentiate NMDA currents in the hippocampus. This study examined the effect of Src on different NMDAR subtypes. My hypothesis was that Src can induce up-regulation of current in NR2A and NR2B containing receptors. To characterize current conducted by different NMDAR subtypes preferential inhibitors NVP-AAM077 and Ro 25-6981 were separately applied to cultured neurons. In SFK activator peptide treated neurons the up-regulated component of NMDA current was partially sensitive to inhibition by Ro 25-6981 and NVP-AAM077. The analysis of inhibition lead me to the conclusion that Src-induced potentiation of NMDA current is mediated by NR2B-type receptors and other non-NR2B NMDAR subtypes. This implies that Src-induced up-regulation of NMDA current is not a property of a particular NMDAR subtype. Treatment of neurons with Ro 25-6981  before the activator peptide application prevented NMDAR potentiation. This implies that NR2B-type receptors are required for the initiation of Src-induced NMDARs potentiation.
Notes: Source: Masters Abstracts International, Volume: 45-06, page: 3065.

Thesis (M.Sc.)--University of Toronto, 2007.

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