Development and Application of Two-photon Excitation Stimulated Emission Depletion Microscopy for Superresolution Fluorescence Imaging in Thick Tissue
Contributors: Sabatini, Bernardo L.; Regehr, Wade G.; Cohen, Adam E.; Boas, David A.
Published: 2013
Language: eng
Description: Two-photon laser scanning microscopy (2PLSM) allows fluorescence imaging in thick biological samples where absorption and scattering typically degrade resolution and signal collection of 1-photon imaging approaches. The spatial resolution of conventional 2PLSM is limited by diffraction, and the near-infrared wavelengths used for excitation in 2PLSM preclude the accurate imaging of many small subcellular features of neurons.
Notes: Keywords: microscopy, superresolution, synapse.

Thesis Ph.D. Harvard University 2013