Poly (ADP-ribose) polymerase
Subtitle: methods and protocols
By: edited by Alexei V. Tulin
Series: Methods in molecular biology -- 780; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 780.; Springer protocols
Publisher: Humana Press
Published: 2011
Place of publication: New York
Language: eng
Number of pages: 521
ISBN-13: 9781617792694; 9781617792700
Subjects: Life sciences; ADP-ribosylation; Cytology; Proteomics; Laboratory Manuals; Poly(ADP-ribose) Polymerases
Notes: Includes bibliographical references and index.
Table of contents: title: Approaches to detect PARP-1 activation in vivo, in situ, and in vitro  -- Girish M. Shah ... [et al.]; title: Quantification of poly(ADP-ribose) in vitro : determination of the ADP-ribose chain length and branching pattern  -- Rafael Alvarez-Gonzalez and Myron K. Jacobson; title: Assay for protein modification by poly-ADP-ribose in vitro  -- Opeyemi A. Olabisi and Chi-Wing Chow; title: Identification of distinct amino acids as ADP-ribose acceptor sites by mass spectrometry  -- Florian Rosenthal ... [et al.]; title: Noncovalent protein interaction with poly(ADP-ribose)  -- Maria Malanga and Felix R. Althaus; title: Noncovalent pADPr interaction with proteins and competition with RNA for binding to proteins  -- Yingbiao Ji; title: Affinity-based assays for the identification and quantitative evaluation of noncovalent poly(ADP-ribose)-binding proteins  -- Jean-Philippe Gagné ... [et al.]; title: Detecting and quantifying pADPr in vivo  -- Yi-Chen Lai ... [et al.]; title: Purification of recombinant poly(ADP-ribose) polymerases  -- Jean-Christophe Amé ... [et al.]; title: Methods for purification of proteins associated with cellular poly(ADP-ribose) and PARP-specific poly(ADP-ribose)  -- Jennifer E. Rood, Anthony K.L. Leung, and Paul Chang; title: Purification and characterization of poly(ADP-ribosyl)ated DNA replication/repair complexes  -- Cynthia M. Simbulan-Rosenthal, Dean S. Rosenthal, and Mark E. Smulson; title: PARP1 genomics : chromatin immunoprecipitation approach using anti-PARP1 antibody (ChIP and ChIP-seq)  -- Niraj Lodhi and Alexei V. Tulin; title: Purification of human PARP-1 and PARP-1 domains from Escherichia coli for structural and biochemical analysis  -- Marie-France Langelier ... [et al.]; title: Regulation of chromatin structure by PARP-1  -- Mi Young Kim; title: Use of PARP inhibitors in cancer therapy : use as adjuvant with chemotherapy or radiotherapy ; use as a single agent in susceptible patients ; techniques used to identify susceptible patients  -- Sydney Shall ... [et al.]; title: Quantification of PARP activity in human tissues : ex vivo assays in blood cells and immunohistochemistry in human biopsies  -- Eszter M. Horvath, Zsuzsanna K. Zsengellér, and Csaba Szabo; title: Assays for hypermethylation of the BRCA1 gene promoter in tumor cells to predict sensitivity to PARP-inhibitor therapy  -- Ilsiya Ibragimova and Paul Cairns; title: Generation of poly(ADP-ribosyl)ation deficient mutants of the transcription factor, CTCF  -- Dawn Farrar, Igor Chernukhin, and Elena Klenova; title: Phenotypic characterization of Parp-1 and Parp-2 deficient mice and cells  -- Christian Boehler ... [et al.]; title: Poly(ADP-ribosyl)ation pathways in mammals : the advantage of murine PARG null mutation  -- Yiran Zhou and David W. Koh; title: Genetic approaches to targeting multiple PARP genes in a mammalian genome  -- Mirella L. Meyer-Ficca and Ralph G. Meyer; title: Approaches to separate and identify polyADP-ribosylated proteins using poly(ADP-ribose) glycohydrolase-knockout Drosophila  -- Hiroto Nodono ... [et al.]; title: Immunolocalization of poly ADP-ribose on Drosophila polytene chromosomes  -- Anna Sala and Davide F.V. Corona; title: The immunodetection of pADPr in Drosophila tissues : immunostaining of thick sections ; immuno-EM  -- Michal Jarnik; title: Poly(ADP-ribose) metabolism analysis in the nematode Caenorhabditis elegans  -- Jean-Franc̦ois St-Laurent and Serge Desnoyers; title: Genetic dissection of PARylation in the filamentous fungus Neurospora crassa  -- Gregory O. Kothe; title: Purification of the poly-ADP-ribose polymerase-like thermozyme from the archaeon Sulfolobus solfataricus  -- Anna De Maio ... [et al.]; title: Strategies employed for the development of PARP inhibitors  -- Stacie Canan, Karen Maegley, and Nicola Curtin; title: Small-molecule collection and high-throughput colorimetric assay to identify PARP1 inhibitors -- Elena Kotova, Aaron D. Pinnola, and Alexei V. Tulin.