Gene targeting
Subtitle: a practical approach
By: edited by Alexandra L. Joyner.
Contributors: Joyner, Alexandra L.
Edition: 2nd ed.
Series: Practical approach series -- 212
Publisher: Oxford University Press
Published: 2000
Place of publication: Oxford; New York
Language: eng
Number of pages: 293
Subjects: Gene Targeting -- laboratory manuals; Genetic Vectors -- laboratory manuals; Stem Cells -- laboratory manuals; Cell Line -- laboratory manuals; Genetic Engineering -- laboratory manuals; Mice, Transgenic -- laboratory manuals; Mutation -- genetics -- laboratory manuals; Gene targeting -- Laboratory manuals
Genres: laboratory manuals.; Laboratory manuals.
Description: "This new edition of Gene Targeting: A Practical Approach contains a remarkable collection of state-of-the-art protocols as well as thoughtful discussion of how to design a new mouse mutation and analyse the resulting phenotype to maximise the information gained. The techniques described for gene targeting in mouse ES cells, are equally applicable for gene targeting in other tissue culture cells, including human cells. The second edition describes additional genetic approaches for studying gene function in mice, including chimera analysis, gene trap (GT) approaches and powerful ways to combine gene targeting with classical genetics." "Gene Targeting: A Practical Approach (second edition) is a practical tool and information resource for scientists working on fundamental or applied aspects of gene targeting, ES cell manipulation, and analysis of mouse mutants. It will be invaluable to all researchers who wish to use the approaches for studies of gene function in cells or mice."--Jacket.
Notes: Includes bibliographical references and index.
Table of contents: title: Gene targeting, principles, and practice in mammalian cells / Paul Hasty, Alejandro Abuin, and Allan Bradley; title: Site-specific recombination in cells and mice / Susan M. Dymecki; title: Production of targeted embryonic stem cell clones / Michael P. Matise, Wojtek Auerbach and Alexandra L. Joyner; title: Production of chimeras by blastocyst and morula injection of targeted ES cells / Virginia Papaioannou and Randall Johnson; title: Production and analysis of ES cell aggregation chimeras / Andreas Nagy and Janet Rossant; title: Gene trap strategies in ES cells / Wolfgang Wurst and Achim Gossler; title: Classical genetics and gene targeting / Scott Bultman and Terry Magnuson.