Metalloproteins
Subtitle: methods and protocols
By: edited by Juan C. Fontecilla- Camps, Yvain Nicolet
Series: Methods in molecular biology -- 1122; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 1122.; Springer protocols (Series)
Publisher: Humana Press
Published: 2014
Place of publication: New York
Language: eng
Number of pages: 299
ISBN-13: 9781627037938; 9781627037945
Subjects: Metalloproteins; Laboratory Manuals
Work titles: Metalloproteins (Fontecilla- Camps)
Description: Metalloproteins are involved in many key biological processes such as gas transport and metabolism, photosynthesis, cell respiration, the Krebs cycle and many other vital redox reactions. Metalloproteins: Methods and Protocols addresses multiple aspects of metalloenzyme research from the production and purification of metalloproteins using both standard recombinant techniques and a cell-free system, to the electrochemical analysis of metalloproteins, as well as IR techniques, Mössbauer spectroscopy and the theoretical interpretation of metalloenzyme catalytic and redox processes, to name a few topics. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Metalloproteins: Methods and Protocols seeks to serve both professionals and novices interested in the metalloprotein field.- -
Notes: Includes bibliographical references and index.
Table of contents: title: Advances in Research on Metalloproteins --; title: Purification of O2-Sensitive Metalloproteins --; title: Expression and Purification of NifB Protein from Aerobic and Anaerobic Sources --; title: Techniques for the Production, Isolation and Analysis of Iron-Sulfur Proteins --; title: Cell-Free Synthesis of the H-cluster: a Model for the In Vitro Assembly of Metalloprotein Metal Centers --; title: Electrochemistry of Metalloproteins Protein Film Electrochemistry for the Study of E. coli [NiFe]-Hydrogenase-1 --; title: FTIR Spectroscopy of Metalloproteins --; title: Characterizing Millisecond Intermediates in Hemoproteins Using Rapid-Freeze-Quench Resonance Raman Spectroscopy --; title: A Practical Guide for Nuclear Resonance Vibrational Spectroscopy (NRVS) of Biochemical Samples and Model Compounds --; title: Study of Metalloproteins Using Continuous Wave Electron Paramagnetic Resonance (EPR) --; title: Mssbauer Spectroscopy --; title: X-Ray Absorption Spectroscopy of Metalloproteins --; title: X-Ray Crystallographic Studies of Metalloproteins --; title: Quantum Mechanical Methods for the Investigation of Metalloproteins and Related Bioinorganic Compounds --; title: Density Functional Theory Broken Symmetry (DFT-BS) Methodology Applied to Electronic and Magnetic Properties of Bioinorganic Prosthetic Groups.