The complement system
Subtitle: methods and protocols
By: edited by Mihaela Gadjeva, Division of Infectious Diseases, Brigham and Women's Hospital, Harvard medical School, Harvard University, Boston, MA, USA
Series: Methods in molecular biology -- 1100; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 1100.; Springer protocols (Series)
Publisher: Humana Press; Springer
Published: 2014
Place of publication: New York
Language: eng
Number of pages: 383
ISBN-13: 9781627037235; 9781627037242
Copyright date: 2014
Subjects: Complement (Immunology); Laboratory manuals; Complement System Proteins; Laboratory Manuals; Complement Activation; Complement Receptors; Komplement (Immunologie)
Notes: Includes bibliographical references and index.
Table of contents: title: Overview  -- Mihaela Gadjeva; title: Evaluation of complement function by ELISA  -- Anja Roos and Jörgen Wieslander; title: Classical complement pathway component C1q : purification of human C1q, isolation of C1q collagen-like and globular head fragments and production of recombinant C1q-derivatives. Functional characterization  -- Mihaela Kojouharova; title: Classical complement pathway components C1r and C1s : purification from human serum and in recombinant form and functional characterization  -- Véronique Rossi ... [et al.]; title: Expression and purification methods for the production of recombinant human complement component C2  -- Angela W. Norton ... [et al.]; title: Purification and characterization of human and mouse complement C3  -- Marieta M. Ruseva and Meike Heurich; title: Purification of human complement protein C5  -- Lars Sottrup-Jensen and Gregers Rom Andersen; title: Quantification of complement C5b-9 binding to cells by flow cytometry  -- Oren Moskovich and Zvi Fishelson; title: Challenges and opportunities in fractionation of recombinant human mannan-binding lectin  -- Thomas Vorup-Jensen; title: Genotyping of FCN and MBL2 polymorphisms using pyrosequencing  -- Lea Munthe-Fog, Hans Ole Madsen, and Peter Garred; title: Assay for estimation of the functional activity of the mannan-binding lectin pathway of the complement system  -- Troels R. Kjaer and Steffen Thiel; title: Purification, measurement of concentration, and functional complement assay of human ficolins  -- Misao Matsushita ... [et al.]; title: Purification and functional analysis of human properdin  -- Joseph O'Flynn ... [et al.]; title: Purification and functional characterization of C4b- binding protein (C4BP)  -- Frida C. Mohlin and Anna M. Blom; title: Purification and functional characterization of factor I  -- Sara C. Nilsson and Anna M. Blom; title: C1 inhibitor : quantification and purification  -- Lilian Varga and József Dobó; title: Purification, quantification, and functional analysis of complement factor H  -- Bing-Bin Yu ... [et al.]; title: Factor H-related proteins  -- Mihály Józsi and Seppo Meri; title: Functional evaluation of factor H genetic and acquired abnormalities : application for atypical hemolytic uremic syndrome (aHUS)  -- Lubka T. Roumenina ... [et al.]; title: Anti-factor H autoantibodies assay  -- Marie-Agnès Dragon-Durey ... [et al.]; title: Purification of native surfactant protein SP-A from pooled amniotic fluid and bronchoalveolar lavage  -- Najmunisa Karbani ... [et al.]; title: Purification of surfactant protein D (SP-D) from pooled amniotic fluid and bronchoalveolar lavage  -- Eswari Dodagatta-Marri ... [et al.]; title: Functional analysis of C5a effector responses in vitro and in vivo  -- Christian M. Karsten, Yves Laumonnier, and Jörg Köhl; title: Detection of complement receptors 1 and 2 on mouse splenic B cells using flow cytometry  -- Luke R. Donius and John H. Weis; title: Quantification of complement receptor 2 calcium signaling enhancement using flow cytometry  -- Luke R. Donius and John H. Weis; title: Purification of C1q receptors and functional analysis  -- Berhane Ghebrehiwet and Ellinor I.B. Peerschke; title: Detection of cell membrane-bound CD46 using flow cytometry  -- Martin Kolev and Claudia Kemper; title: Detection of CD59 using flow cytometry  -- Martin Kolev; title: Functional analysis of CD59 using complement-dependent cytotoxicity assay  -- Martin Kolev; title: Detection of soluble CR3 (CD11b/CD18) by time-resolved immunofluorometry  -- Gitte Krogh Nielsen and Thomas Vorup-Jensen; title: Analysis of the complement activation in mice  -- Nirmal K. Banda and Kazue Takahashi; title: Opsonophagocytic assay -- Markryan Dwyer and Mihaela Gadjeva.