Molecular characterization of the bovine torovirus:  The complete sequence and cloning of the Breda-1 virus genome.
Published: 2004
Language: eng
Number of pages: 123
Description: Breda virus (BRV), genus Torovirus, family  Coronaviridae, has been established as an etiological agent of diarrhea in cattle. In this study, BRV-1 RNA was amplified using long RT-PCR and the genome sequence determined by sequencing the amplicons. 20,920 nucleotides of novel sequence were obtained. Sequence analysis revealed the presence of a large viral polymerase gene composed of two overlapping ORFs, 1a and 1b. The 1a ORF contained two protease domains with homology to the arteriviruses, picornaviruses, and coronaviruses, and another domain found only in the Coronaviridae. The 1a/1b overlap region contained sequence elements involved in ribosomal frameshifting, and the putative replicase and helicase domains were identified in ORF 1b. 16 kb of the 3'  end of the genome were cloned, and re-sequencing and analysis of this region suggests a quasi-species nature of this virus. This sequence, to our knowledge, represents the first account of a complete torovirus genome sequence.
Notes: Advisers:  Martin Petric; Raymond Tellier.

Thesis (M.Sc.)--University of Toronto, 2004.

Electronic version licensed for access by U. of T. users.

Source: Masters Abstracts International, Volume: 42-06, page: 2107.

MICR copy on microfiche (2 microfiches).