Biological aging
Subtitle: methods and protocols
By: edited by Trygve O. Tollefsbol, Department of Biology, University of Alabama at Birmingham, Birmingham, AL, USA
Contributors: Springer Science+Business Media
Edition: Second edition.
Series: Methods in molecular biology -- 1048; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 1048.; Springer protocols (Series)
Publisher: Humana Press
Published: 2013
Place of publication: New York
Language: eng
Number of pages: 354
ISBN-13: 9781627035552; 9781627035569
Copyright date: 2013
Subjects: Aging; Laboratory manuals; Longevity; Laboratory Manuals; Physiology; Investigative Techniques
Description: The second edition of Biological Aging: Methods and Protocols expands upon the previous edition with current, detailed, useful and promising methods currently available to study aging. With new chapters on protocols that detail aging cell culture as well as many more contemporary approaches such as nuclear transfer, microarray and proteomics technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Biological Aging: Methods and Protocols, Second Edition details the most recent breakthroughs in technology that have served to advance the study of aging.
Notes: Includes bibliographical references and index.
Table of contents: title: Cell Senescence Culturing Methods / Huaping Chen, Yuanyuan Li, and Trygve O. Tollefsbol; title: Digital Image Analysis of Cells Stained with the Senescence-Associated b-Galactosidase Assay / Liran I. Shlush and Sara Selig; title: Analysis of Biomarkers of Caloric Restriction in Aging Cells / Yuanyuan Li and Trygve O. Tollefsbol; title: Cell Sorting of Young and Senescent Cells / Graeme Hewitt, Thomas von Zglinicki, and Joã̃o F. Passos; title: Studying the Replicative Life Span of Yeast Cells / David A. Sinclair; title: Methods for Creating Mutations in C. elegans That Extend Lifespan / Dayong Wang, Min Cao, Jessica Dinh, and Yuqing Dong; title: Aging Studies in Drosophila Melanogaster / Yaning Sun, Jason Yolitz, Cecilia Wang, Edward Spangler, Ming Zhan, and Sige Zou; title: The Use of Calorie Restriction Mimetics to Study Aging / Munehiro Kitada and Daisuke Koya; title: Using Somatic-Cell Nuclear Transfer to Study Aging / Satoshi Kishigami, Ah Reum Lee, and Teruhiko Wakayama; title: Induction of Cellular Senescence by Oncogenic RAS / Romi Gupta and Narendra Wajapeyee; title: Methods of Cellular Senescence Induction Using Oxidative Stress / Zhe Wang, Dandan Wei, and Hengyi Xiao; title: Methods of Testing Pharmacological Drugs Effects on Aging and Life Span in Mice / Vadimir N. Anisimov, Irina G. Popovich, and Mark A. Zabezhinski; title: Mitochondria-Targeted Antiaging Gene Therapy with Adeno-associated Viral Vectors / Dejia Li and Dongsheng Duan; title: Real-Time Bioluminescence Functional Imaging for Monitoring Tissue Formation and Regeneration / Nadav Bleich Kimelman, Ilan Kallai, Dmitriy Sheyn, Wafa Tawackoli, Zulma Gazit, Gadi Pelled, and Dan Gazit; title: Exometabolomic Mapping of Caenorhabditis elegans: A Tool to Noninvasively Investigate Aging / Robert J. Mishur, Jeffrey A. Butler, and Shane L. Rea; title: Detecting Polymorphisms in Human Longevity Studies: HLA Typing and SNP Genotyping by Amplicon Sequencing / Gilberto Vargas-Alarcón and Carmina Flores-Domı́nguez; title: Gel Electrophoresis-Based Proteomics of Senescent Tissues / Steven Carberry and Kay Ohlendieck; title: Single-Cell Semiconductor Sequencing / Andrea B. Kohn, Tatiana P. Moroz, Jeffrey P. Barnes, Mandy Netherton, and Leonid L. Moroz; title: Application of DNA Microarray Technology to Gerontological Studies / Kiyoshi Masuda, Yuki Kuwano, Kensei Nishida, and Kazuhito Rokutan; title: Epigenetic Biomarker to Determine Replicative Senescence of Cultured Cells / Carmen M. Koch and Wolfgang Wagner; title: Single-Neuron Transcriptome and Methylome Sequencing for Epigenomic Analysis of Aging / Leonid L. Moroz and Andrea B. Kohn