CellProfiler/CellProfiler — Issue #297: ReassignObjectNumbers Per_parent does not always assign child objects to the correct parent
Author: dlogan
Created: 2012-02-29 20:23:36+00:00
State: open
Labels: Bug

Here's a screenshot of the problem:
http://cl.ly/322q1M0L3A0e1N380a1E
On the left is a RelateObjects output that shows 6 parent objects (cytoplasm) and many children mitochondria. However, when I then try to reassign all children mito of a single cytoplasm (right side), the assignment is not correct, collapsing the objects to just two parent objects. In other images, this method seems to work OK, though.

Input folder: \\iodine\imaging_analysis\2007_05_14_Mitochondria_AnneCataldo\McPhie\images\2011_03_02_images\MitoRaw_ActinLines

Pipeline:
https://imageweb.broadinstitute.org/imaging/analysis/2007_05_14_Mitochondria_AnneCataldo/McPhie/Pipelines/PIPE_2012_02_20_WholeCell.cp

Choose Image Cycle that has "...L 68 19_c..." in it.