nolanlab/spade — Issue #18: Feature request - saving a tree scaffold/template for future use?
Author: mleipold
Created: 2011-12-05 21:38:34+00:00
State: open

Here at the Stanford HIMC, we have a few multi-year customer studies. We would get the 2009 samples, process them, then repeat each year as the 2010, 2011, 2012, etc samples come in.

Is there currently a way to build a tree with a given panel of markers starting with, say, the 2009 samples, and as each year's samples comes in, perform SPADE analysis on them using the *same* tree scaffold/template of the 2009 samples?

Currently, the only way I know how to make everything appear on identically-framed trees is to include all the FCS files in the same tree-building exercise. This would mean that each year, we would have to rebuild our tree. While the trees built on the same markers should be *similar*, they would not necessarily be *identical* each time the analysis is rerun.

Kinda like how in FlowJo/etc, you build a template of your analysis, and can just keep dragging new FCS files in (though in the case of SPADE, I obviously wouldn't be asking to readjust gates).