CellProfiler/CellProfiler — Issue #165: JIRA [IMG-918] Current functionality of Load Images/grouping may not allow for creating z-stacks
Author: thouis
Created: 2011-11-23 20:50:28+00:00
State: open
Labels: current

The way many microscopes save z-stacks may not be compatible with the way LoadImages/grouping works right now: see /Volumes/imaging_analysis/People/Kate/TestZstacking for an example. The issue is, you likely have two channels, saved in separate directories (one named DAPI and one named YFP in this example), but the contents of each directory are named identically. Thus loading each image based on matching something in the *file name itself* doesn't work- you need to be able to match based on the directory as well, and CellProfiler needs to be OK with the Filename_DAPI and Filename_YFP being the same.

Original reporter: klmadden
Jira link: https://jira.broadinstitute.org/browse/IMG-918
Fix version: Current
Assignee: leek

**Comment from anne on Fri, 16 Jul 2010 10:46:32 -0400:**
This isn't a z-stack issue, it is a filename issue - the subfolder needs to be considered as part of the name when deciding whether two files are actually identical.