CellProfiler/CellProfiler — Issue #142: JIRA [IMG-1077] Normalize multi-channel images equally
Author: thouis
Created: 2011-11-23 20:50:21+00:00
State: open
Labels: current

Images viewed in CP default to the contrast setting = Normalized. However multi-channel images are apparently not scaled equally between the channels. For example:
/Volumes/imaging_analysis/2007_05_14_Mitochondria_AnneCataldo/McPhie/XYZ16040910/X1\ P6\ 040910\ 1.6\ 1_c1.TIF
This image is really almost all blue (nuclei), however this is only apparent when viewed as Raw. The red background in the Normalized view is all just noise. (I realize that the collaborators really should have saved it as grayscale, but they saved it as color manually :/). Regardless, it seems to me that we would always want to scale the channels equally. If there's a good reason not to, maybe a checkbox for sync'ing them?

Original reporter: dlogan
Jira link: https://jira.broadinstitute.org/browse/IMG-1077
Fix version: Current
Assignee: afraser