CellProfiler/CellProfiler — Issue #111: JIRA [IMG-1196] adapt idprim to use watershed on gradient img
Author: thouis
Created: 2011-11-23 20:50:11+00:00
State: open
Labels: current

see Carolina's thesis

Original reporter: klmadden
Jira link: https://jira.broadinstitute.org/browse/IMG-1196
Fix version: Current
Assignee: leek

**Comment from leek on Fri, 19 Nov 2010 14:13:45 -0500:**
We agreed to use this method as an initial thresholding and not as a segmentation:
Find the local minima and maxima using the criteria used to find local maxima in declumping.
Calculate the gradient on the smoothing of the original image or on an image provided by the user.
Perform the watershed and classify pixels depending on whether the watershed puts them in an area associated with a local maximum (foreground) or local minimum (background).
The thresholding could then be used in IdentifySecondary and ApplyThreshold.