lh3/wgsim — Issue #2: read simulation for reverse and forward offset is not consistent.
Author: jozerffer
Created: 2011-11-14 06:58:57+00:00
State: open

Hi,

As mention above in the title. Read simulation for reverse and forward mutation offset is not consistent. Please check.

Example:

gi|224589813|ref|NC_000021.8|_9440728_9441261_0:0:1_0:0:0_42167/2 (forward)
ATGTCAAGATAATGTCAGAAATTCTTTACAATTGCTTCCAGAAGGAGTAGCCTTTTGATCTAGTGCACAGGTGTCCAGTC (TTTTA) GGCTTCTTAGGGCCA
IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII

@gi|224589813|ref|NC_000021.8|_9440248_9440824_0:0:0_0:0:1_93e94/1 (reverse)
GCCCTAAGAAGCC (ATAAA) GACTGGACACCTGTGCACTAGATCAAAAGGCTACTCCTTCTGGAAGCAATTGTAAAGAATTTCTGACATTATCTTGACATGA
IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII

simulated insert
gi|224589813|ref|NC_000021.8| 9440811 - A +