An action potential arrives at the presynaptic terminal causing voltage gated calcium ion to open, increasing the calcium ion permeability of the presynaptic terminal cell membrane. Calcium ions enter the presynaptic terminal and cause vesicles to release their neurotransmitter acetylcholine from the synaptic vesicles into the presynaptic cleft. Diffusion of acetylcholine across the synaptic cleft and binding of acetylcholine to acetylcholine receptors on the postsynaptic muscle fiber membrane causes an increase in the permeability of ligand gated sodium ion channels. The movement of sodium ions into the muscle cell results in depolarization of the postsynaptic membrane. Once threshold has been reached, a postsynaptic action potential is generated and is propagated over the muscle cell membrane. Acetylcholine is rapidly broken down to acetic acid and choline in the synaptic cleft by the enzyme acetylcholine esterase. The choline is reabsorbed by the presynaptic terminal and combined with acetylcholine to form more acetylcholine, which enters the synaptic vesicles. Acetylcholine is a very important part of the process of creating a new solution to the process of creating a new solution. Come on Amy, get up! Show me some moves Amy! You want me to put some music on for you? Right! For severe knee pain, we do the first one so it inside the chest. For severe knee pain, we do the first one so it inside the knee and outside the knee. For more severe pain, go up the patella and then below, that should be comfortable. And then once again, you apply a tie-over to the top. The case of the knee, but it's on for a long period of time, you would use pieces of hypersyxin instead of the transpor. Here the knee placement, a lot of pain is medially. We don't go right over the condyle, we come a little bit more anteriorly so you can get to the faces of the joint. So for mild pain medially and literally, just onto the outside of the knee cap. So that's the lateral placement, that's the medial placement and then above and below the patella. So just here, issue the givicle of the tibia and then above just above the knee right over the pre-patella bursa.