Welcome to the chemistry lab. Today we will show you how to prepare your BRF Brite titration. My name is Brian Ries-Wattis and I'm a biochem major from the class of 2018. We will outline the procedure from obtaining your BRF to washing and measuring the initial volume inside it. We hope that this tutorial will help you out in lab. Now sit back relax and let's get started. If your RF is a laboratory apparatus that is used to quantitatively measure the volume of a liquid or a gas. The BRF consists of a long graduated glass tube that dispenses liquid. The amount of liquid that is dispensed from it is controlled by a stopcock which is located at the bottom of your BRF. Your rats are typically used in titration experiments. This is where a solution with a known concentration is used to determine the concentration of another compound. In order to properly use a BRF, proper rinsing and washing must be done in order to ensure there are no molecules or particles that could potentially interfere with what is being measured. Washing is essential. Never assume your glassware is clean. To do so, rinse your BRF out one to two times with distilled water. This will cause any particles on the BRF to go into solution and will be washed away. Once you have filled the BRF, turn the stopcock once and allow some of the water to run through the dispensing tip. This allows any air bubbles that are trapped to be dispelled. Once you have done this, turn the stopcock to the closed position and drain the rest of the water from the top of the BRF into the sink or a beaker. Once you have finished rinsing it with DI water, rinse out your BRF once with your titrant and repeat the same procedure as with water. Let's now coat the inside of the BRF with the titrant. Make sure the stopcock is in the closed position with the stopcock perpendicular to the glassware or about 5ml of titrant using a long stem glass funnel into the BRF and hold the BRF on its side. You do not need to fill your BRF to the top just yet. Using your hands, rotate the glassware providing maximum coverage for the titrant to coat the inside surface of the glassware. In this case, the titrant being used is sodium hydroxide. The last step of washing is essential if you need to be very precise when calculating the concentration of the titrant. Now, dispense the last washing into a waste beaker. Once you are finished with washing, you are now ready to set up your BRF using a long stem glass funnel and sliding the BRF under eye level carefully pour your titrant into the BRF to the desired volume. If you cannot get the BRF to eye level before filling it, you may remove it from the clamp and hold the BRF to eye level instead. When doing this, make sure you are very cautious about spilling any titrant on yourself or others. When reading the initial volume of the BRF, make sure you take the reading at the bottom of the meniscus. For opaque liquids, like potassium permanganate, use a flashlight to get a better view of the meniscus. If you have overfilled your BRF past the graduations, you may turn your stopcock just a little bit to dispel some liquid. Upon reading the initial BRF volume, adjust the BRF height so that there is ample room underneath the BRF to place an early Meyer flask with the strip pad underneath it. You may have to adjust the height again to avoid any splashing when dispensing the titrant. Your BRF is now ready for your experiment. Thank you for listening and I hope that this video will strengthen your BRF preparation skills.