Hi everyone. Today we're going to be looking at a lab dealing with diffusion and azmolary. The first experiment we're going to do deals with finding out the azmolary of a potato, which basically is the same as asking, what is the concentration of stuff inside the cells of these potatoes? Okay, so we're going to do this by incubating these potatoes in different solutions of sucrose. Okay, and so by looking at the changes in these potatoes over time, we will be able to figure out the concentration inside here. Okay, so we have a different set of mass solution. We have a bunch of different solutions here. We have zero molar sucrose or just pure water. We have 0.1 molar sucrose, 0.2 molar sucrose, 0.3, 0.4 molar, 0.5 molar, and 0.6. Now this one here, pure water, obviously is going to be a hypotonic solution compared to what's inside these potatoes because pure water has no solutes in it, right? So no matter what you put into this water, water is always hypotonic. So one of the potato pieces is going to go into the water. Now we have a whole range here, and so at the end of this range we have 0.1 molar sucrose solution, which is the high concentration, sorry, 0.6 molar solution. We'll edit that up, right? We want the hair. So 0.6 molar solution is a fairly high concentration, and so when you put that potato into this, the solution in here is going to be hypertonic, so it's going to be much more concentrated than is inside the potato zone, okay? So if a wide range, and so somewhere in between 0 molar and 0.6 molar, is a solution that would be isotonic to these cells, and so it is that solution that will tell us what is the concentration of stuff or solutes inside of these cells, okay? So what we have here are a bunch of these potato cores that we're going to first weigh, and so I'm going to turn on the balance, my love the assistant spoke to turn on the balance, okay? Okay, so okay, so first piece of potato, really exciting stuff here, is 15.9 grams. I'm going to write that down as my initial weight, 15.9 grams, and so I'm going to place that into the first bottle, so let's find out into ball number one, my di-night water, just drop it in there, and leave it alone, let's take that deal number two, has 17.0 grams, and we'll place that into the second bottle, so always keep track of these things, don't mix them up, don't just weigh them and just leave them on a piece of paper or something, just weigh them and put them directly into the bottles, so 15.4 grams, now you'll notice that all of these are different sizes and different weights, and so we will adjust for that with our calculations later on, okay? So 16.2 grams goes into point three molar, 14.4 grams, 13.8 grams, as it is 0.5 molar, and last one is 14.6 grams, okay? 16.6 grams, and so now we wait.