Imagine that this glove is a molecule and this one is another molecule identical to the previous one when reflected in a mirror. This mirror image relationship is called chirality. In chemistry, these molecules are called enantiomers and are often mixed with each other in a solution like a huge sea full of left and right gloves. Imagine now that you are only interested in separating one type. For example, the gloves that fit your left hand. How would you go about it? Self-assembled mono-layers using chiral molecules can provide surfaces designed specifically to attract one of the enantiomers in a mixture or in your imagination one of the two types of gloves. This method separates the two forms of the molecule, fixing one of them to one that interests you on this surface. Our project aim to use self-assembled mono-layers to act as templates for crystal growth of molecular combinations called complexes. We created nano-scale spaces in mono-layers for recognition. We observed the segregation of enantiomers at molecular level and we achieved template growth at these nano-structured surfaces. Our results led us to develop rules for the specific attachment of molecules to surfaces.