Liquid liquid extraction is a very important step to reduce or to fractionate some groups of compounds. We follow the polarity. Well here we have prepared the aqua slayer. This is the aqua slayer. It is constituted from we have methanol and also water about 30% of water and 70% of ethanol. In fact we've already prepared this is a crude extract basically so the crude extract was prepared from by methanol as an extractor agent then we dissolve the methanol and 30% and 70% so the crude extract methanol extract was dissolved in 70% methanol and 30% water. So why would add the water in fact to create the aqua slayer because we will add some Hicksane Iowad the Hicksane here then I'll add the chloroform the etyl acetates and try to make some fractionation as I said so we will follow the idiotic series another to reduce the complexity of mixture because when we talk about a crude extract we have a hundred of molecules and we need to isolate them it is very hard to isolate them so we there are a lot of steps that we need to proceed to get a good separation right now I'll add Hicksane. So you will realize the creation of two different lyer here and I didn't shake yet but you will see how it works well okay yes like that here we have two lyers so the top one is the organic and the down part is the down fraction is the aqua slayer. Let's just if we didn't add the water here and the methanolic extract we took the methanolic extract and we dissolved it in methanol then we added the Hicksane. So we will be mix it together we will not have two faces okay and another to have two different phases it is very important to add the water to increase the the density of this fraction to be down then when we add any fraction they will be not together we didn't shake yet but we have to shake to shake very very well in order to have to increase the surface the surface of contact between the organic and aqua slayer. We used the stutter this is the stutter we have two clumps of stutter and funnel we have to do that we can never sense then we reverse the stutter to funnel like the hicksane without having the top to be getting because we have some guesses and we have to reduce the top and we put the stutter to the bottom and then we move the stutter so this is the surface of contact between the twerganic liars and here we go