Hi, hello everyone, welcome back again and now I am Rabin with you and you are watching BySenseMania Ok, so now today I want to discuss with you about staining solution and preservatives Ok, let's go So basically first of all I am going to tell you about staining solution You are the only one who has an active partner, you are the only one who has an active partner Next is the preservative solution, the preservative solution, the formaldehyde solution and the formic acid acid is the only one who is important, so now first of all I am going to tell you about lacto phenyl cotton blue ok, so lacto phenyl cotton blue is the mixture of phenyl crystal, cotton blue, glidesyl, lactic acid and distilled water ok and one thing I want to tell you that is lacto phenyl always in blue in color ok and use of lacto phenyl always it is used in weight mount preparation for visualization of algal fungal and other microbes still, I mean in this way algal fungal by one microbe still they both but other destructur they both are the same, so they are the lacto phenyl cotton blue solution used in this way ok, so now ever tell you about chemical composition, chemical composition of lacto phenyl is in a 100 ml solution cotton blue is 0.05 gram phenyl crystal, 20 gram glysyrel, 40 ml, lactic acid, 20 ml and distilled water is 20 ml ok and noted below one thing here that is however lacto phenyl and cotton blue use separately ok, where cotton blue act as staining and lacto phenyl as mounted, so now second important staining solution is saffronin ok, so saffronin is a biological stain and crystal rating color ok, it color to mean the red but chip crystal act clear color red ok, it is using counter stain in some staining protocol, after staining the cell newly become red in color, study about histology and cytology, so histology and cytology we have already put slide at this course to tell you about both gram staining in bacteria, so gram staining, negative gram positive bacteria ok, stain could to saffronin use color and one thing this is redox indicator redox indicator is also chemistry they color ok, so now we talk about histology and cytology, so what is histology, study about structure and function of tissue this is called cytology and cytology study about structure and function of cells, this is called cytology. So now we talk about piezer weighty ok, so piezer weighty mainly, we have to say biological organism ok, piezer could act as a shit ok, we have to piezer weighty ok and this process and if putdutitaka putdutaka ballahay piezer vision ok, so you can have two piezer weighty, we have to measure the formula date solution, a bomb formula formula date solution, so formula date solution, the number of solutions is also in both the two factors just a solution, so commercial formula date is like a bomb water ok, so commercial formula date is like 12.5 and extra ml is the solution, so it's like 12.5 ml is like commercial formula date is like 87.5 ml is like water, so now we talk about formula Acetic Acid Nail, Action Mell Formal Acetic Acid Nail, Commercial Formal Date 5mm, Glacial Acetic Acid 5mm, And Ethanol Thug Nail 90 ml. And one thing noted here that is Formal Acetic Acid is used for long time preservation. So, in the next show, we will see practical lab. If you like this video, please like, share and subscribe to our channel.