Okay, so we're going to use Portable Water Cove today just to sterilize this sample of water. Before you starting to make sure it's labelled with your name and what it is and has put my initials on and the star is still water. Then pop it into the Portable Water Cove. The water Cove itself needs water in the base to just above the pan inside. So to put some water in there like that. That should be okay. Alright, so for each process you need to ensure sterilization. It's not necessarily every single run but occasionally you need to use a sterility test indicator like this. Just pop it inside a beaker and pop it in with the items you want to sterilize. Now you're ready to put the lid on. Which is how you use... The two fixed wing nuts on the lid go on the two without wing nuts on the base, the old pipe. Just firmly clip it one down. You can sure that tight. Then go round the others. Opposite. Using the two opposite each other rather than going round. Like so. And you've done all those. Just make sure you've got them all tight and you're ready to light the gas. Now light the gas. Once you've started the gas you must make sure that this water valve is closed and this valve is open. This is to vent the air out as the waterclaves heat up. The air will be venting out this valve ensure that there's steam to sterilize whatever you got in your waterclave. Okay, now I'll switch the gas on. The atmosphere inside the waterclave will heat up and the water will heat up. And once it gets to 100 on this dial it will vent out of this open valve. The reason for this is you need to purge the air inside the waterclave so that the atmosphere becomes steam rather than hot air. This will ensure proper sterilization of the items you've got inside. Once you've had a couple of minutes of 100 degrees you can then close this valve then this pressure will gauge will go up as the pressure inside the waterclave goes up to 121. At this point the steam will be released out of this gauge which will control the pressure inside the waterclave. Once you hear the steam coming out of this gauge you can commence timing of your 15 minutes 121 cycle. Okay, so now the waterclave has been venting for a couple of minutes so we can now close this valve taking care not to touch any other part of the waterclave as it will burn you. After three or four minutes the steam is now venting out of the regulator and the needle has reached the red zone which indicates the waterclaves reached its sterilization temperature. You can now commence timing and you can also turn down the gas to a simmer setting rather than the pool setting which we've had. When the 15 minutes timing is you simply turn off the gas and wait for the waterclave to cool down. The waterclave has cooled to below 100 and the pressure gauge is at least down to zero. It can be emptied. It's preferable to get at least below 80 but you can open it once it's below 100 but you need to. First of all open this valve as you get some negative pressure in the waterclave. This point you need to wear some heat proof drops as well. You undo the wing nuts in the opposite method to doing them up so the two captive nuts which are these two here are done last. These are the last two. The lid will come away as you undo these two. Carefully lift off once you've done the nuts. Remove the contents bearing in mind. They're still very hot and you need time to cool down.