For culturing in liquid media, airline-mire flasks are prepared. The flasks are stoppered with cotton plugs. To keep condensed water away, the plugs are covered with aluminum foil before autoclaving. Color change of the autoclave indicator tape indicates upheating of the autoclave. This also marks the flasks as sterile. For small-culture volume sterile tubes are used. A sterile pipette may be used for transferring sterile medium. Sterile flasks are filled with a medium. For anoculation of a single colony in liquid media, colony material is taken and directly transferred to a sterile tube or an airline-mire flask. Be sure to suspend the colony material properly. Press the loop gently on the glass surface, then move the loop slightly forward and backward in order to get a homogeneous suspension. Samples are then incubated in a laboratory shaker. Most laboratory organisms like E. Coli or Bicillus strains will grow within some hours or overnight.