This book trafficking is fundamental to distribute correctly lipids and proteins among cellular compartments. A complex network of interconnected pathways that form this supreme. Organals to satisfy the cellular demand. Multi-subunite, a varying complex, says MTC's former group of nine protein assemblies that are concerned from yeast to human and that are essential for intercellular trafficking. Exorcist DSL1 called Garbhoff School of Trappin Trappin II and Trapp 3. MTC recognizes a specific type of vesicle into the retune, the appropriate receptor membrane. Provide specificity and directionality through the traffic gain. My research aimed to do a comparative analysis among all MTC's. Right on the mechanism of action on these complexes, my group have developed a PITC protein interaction from imaging of complexes. A high sensitivity technique that allows us to study interactions between two proteins using live cell imaging. First important in silicaogenome, wired screening to identify those proteins that could be functionally related to MTC's. We pair and use PITC for the protein interaction screen with the protein selected in the silica analysis. Even the highest sensitivity of the assay and that he has had done directly in living cells, this method was brought to detect interactions that have been overlooked by previous analysis such as indirect as the interloew abundant interactions found at MTC's established network of interactions with P480 passes lipid for paces that has a K-phospholipid suit, a cytosolistically fluid of the membranes. P480 passes maintain the asymmetric distribution of phospholipid, sand, they also play essential roles in the vesicle trafficking. In his era 5, P480 passes DNF1, DNF2, DNF2, DRS2 and NEOL. DNF1 and DRS2 are the ones that concentrate most of the interactions with the MTC's mainly garp and the traps up to date. No study has observed that MTC's and P480 passes might function together. In his thesis, I investigated the molecular mechanism that mediates the interplay in this network of essential components for the trafficking jury. I found that Garp is responsible to transport DNF1 from late enzymes to transgoalgy network during recycling of DNF1 to the plasma membrane. Is essential to maintain the correct distribution of PE in the plasma membrane furthermore? I found that DRS2 is required for the proper functioning of cytoplasm2vaculotogating CVT pathway amodel historically used to study. So that the lungs are lazy when the CVT pathway is triggered both the traptery and transmembrane protein named ATG9 reached a phagopore assembly site P8. As the supper process, no results of my thesis show that in response to temperature decreases the cell induces the binding between the traptery and the DRS2. That this interaction is required to sustain the adequate transportation of ATG9 to the PAS. In line with this, I have seen DRS2 is fundamental for the biogenesis of vesicles loaded with the ATG9 from the endosytic compartments. All together, the starter of the thesis and you function to this flip base in the CVT pathway that I have important implications in other types of vots of edgy. I identify the short motif in the intermenal tail of the DRS2 that is required for the interaction traptery DRS2. This is a motif highly conserved among P4, ATPAS's send out the proteins that bind MTCs. What suggested it might have a more general role in regulating the trafficking of vesicles that remains to be elucidated?