硼酸鋰緩衝液

硼酸鋰緩衝液（）、LB緩衝液（），是用於瓊脂糖電泳的緩衝溶液，常用於分離核酸，如DNA、RNA。由硼酸鋰（氫氧化鋰一水合物和硼酸）組成。

LB(R) is a registered (USPTO) trademark of Faster Better Media LLC, which owns US patent 7,163,610 covering low-conductance lithium borate polynucleotide electrophoresis. 

Lithium Borate buffer has a lower conductivity, produces crisper resolution, and can be run at higher speeds than can gels made from TBE or TAE (5-50 V/cm as compared to 5-10 V/cm). At a given voltage, the heat generation and thus the gel temperature is much lower than with TBE/TAE buffers, therefore the voltage can be increased to speed up electrophoresis so that a gel run takes only a fraction of the usual time.Brody, J.R., Kern, S.E. (2004): History and principles of conductive media for standard DNA electrophoresis. Anal Biochem. 333(1):1-13.   PDF  Downstream applications, such as isolation of DNA from a gel slice or Southern blot analysis, work as expected with lithium boric acid gels. 
Sodium boric acid: a tris-free, cooler conductive medium for DNA electrophoresis. Biotechniques 36(2):214-215.  PDF Brody, J.R., Calhoun, E.S., Gallmeier, E., Creavalle, T.D., Kern, S.E. (2004): Ultra-fast high-resolution agarose electrophoresis of DNA and RNA using low-molarity conductive media. Biotechniques 37(4):598-602.  PDF

SB buffer containing sodium borate is similar to lithium borate and has nearly all of its advantages at a somewhat lower cost, but the lithium buffer permits use of even higher voltages due to the lower conductivity of lithium ions as compared to sodium ions and has a better resolution for fragments above 4kb.

 參見 
 鋰
 硼酸鹽
 （）
 TBE緩衝液（）

 外部鏈接 
硼酸鈉作為電泳緩衝液 

 參考文獻 

Category:緩衝液