荧光素

荧光素（fluorescein）又称荧光黄，化学名二羟基荧烷，是一种合成有机化合物，外观为暗橙色/红色粉末，可溶于乙醇，微溶于水。在蓝光或紫外线照射下會发出绿色荧光。

在多种技術应用（如荧光抗体技术）中被广泛用作为荧光示踪物。目前常用於眼科的檢查中，如在眼表疾病的临床工作中，也用荧光素钠（fluorescein sodium）进行染色Eom Y, Lee JS, Keun Lee H, Myung Kim H, Suk Song J. Comparison of conjunctival staining between lissamine green and yellow filtered fluorescein sodium. Can J Ophthalmol. 2015 Aug;50(4):273-7. doi: 10.1016/j.jcjo.2015.05.007. PMID 26257220.Wang S, He J, He X, Liu Y, Lin X, Xu C, Zhu L, Kang J, Wang Y, Li Y, Guo S, Zhang Y, Luo Z, Liu Z. AES-CSFS: an automatic evaluation system for corneal sodium fluorescein staining based on deep learning. Ther Adv Chronic Dis. 2023 Feb 12;14:20406223221148266. doi: 10.1177/20406223221148266. PMID 36798527; PMCID: PMC9926379.，后在藍光下观察，以评估角膜屏障功能是否受损及受损程度。

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参考文献

外部链接
Absorption and Emission Spectra of Fluorescein in Ethanol  and Basic Ethanol  at OGI School of Science and Engineering
Fluorescein Ionization Equilibria at Invitrogen
MSDS at Oxford University 
Absorption spectra and fluorescence emission spectra 

Category:荧光酮染料
Category:染色着色剂
Category:荧光染料
Category:激光增益介质
Category:酚
Category:苯甲酸衍生物