oeg/software_benchmark_v2 huggingface dataset:
Immunoblotting was performed using standard protocols with the following primary Abs: Nrf2 (Santa Cruz Biotechnology, Inc.), mouse Nrf2 (gift from D. Tuveson [Cold Spring Harbor Laboratory, Cold Spring, New York] and E. Schmidt [Montana State University, Bozeman, MT]), BRCA1 (Santa Cruz Biotechnology, Inc.), mouse BRCA1 (GH118; gift from J. Jonkers), vinculin (Abcam), -actin (Sigma-Aldrich), H3 (Genetex), Myc (Cell Signaling Technology), GFP (Novus Biologicals), and His (Invitrogen).
At the end of the treatment, cells were incubated with 1mM HE and 10 nM Sytox green nucleic acid stain for 20 min at 37 1C, centrifuged at 100 Â g, resuspended in 100 ml of PBS containing 2% FBS and analyzed immediately using a flow cytometer equipped with 488 nm laser for excitation (Accuri C6, BD Biosciences, San Diego, CA, USA).
All products were amplified by PCR using 50 ng of cDNA and the following specific primers: RIP1, forward 5 0 -GCACCAGCTGTCAGGGCCAG-3 0 , reverse 5 0 -GCCCAGCTTTCGGGCACAGT-3 0 ; RIP3, forward 5 0 -ACCTTGGCTGGCACTC CCCA-3 0 , reverse 5 0 -CCCCTGCCGAACTGTGCTGG-3 0 ; TNFa, forward 5 0 -GCC CACGTCGTAGCAAAC-3 0 , reverse 5 0 -GCAGCCTTGTCCCTTGAA-3 0 ; Iba1, forward 5 0 -CTTTTGGACTGCTGAAAGCC-3 0 , reverse 5 0 -GTTTCTCCAGCATTC GCTTC-3 0 ; and b-actin, forward 5 0 -AGACTTCGAGCAGGAGATGG-3 0 , reverse 5 0 -CCATCATGAAGTGTGACGTTG-3 0 .
To visualize native GFP or mRFP, we collected adult flies 4-6 days after eclosion and brains were dissected in ice-cold 4% paraformaldehyde solution in PBS (1.86 mM NaH 2 PO 4 , 8.41 mM Na 2 HPO 4 , and 175 mM NaCl) and fixed for an additional 60 min at room temperature.
Microscopy experiments typically used 8-well microscope chambered slides and a Nikon Eclipse TE-2000 U epifluorescence microscope equipped with blue DAPI (Exciter: D360/40x, Dichroic: 400DCLP, Emitter: 460/50m), green FITC (Exciter: D360/40X, Dichroic: 400DCLP, Emitter: HQ535/50m), and deep-red Cy5 (Exciter: HQ620/60X, Dichroic: 660LP, Emitter: HQ700/75m) filters.
The Rasch overall score was used as a continuous variable in descriptive, and non-parametric bivariate analyses to explore differences in job satisfaction and age, type of degree (PharmD/Non-PharmD), years of experience, workload (Avg. prescription volume/week, and whether they worked more than 36 hours/week), type of pharmacy chain (Grocery/non-grocery), having experience outside of community practice (yes/no), provision of patient care services at the pharmacy (yes/no) and job position (Pharmacist in charge yes/no) the selection of these variables was based on previous studies.
Appropriate probes [partial for OsSOS1(AK065608) corresponding to its N-terminal part and full length for other genes; OsSOS2 (AK102270); OsSOS3 (AK101368); OsTOC1 (AK111828)] were amplified using the primers listed in Table 1 and radiolabeled using the DecaLabel™ DNA labeling kit, (K0621; Fermentas).
Membranes were blocked with 5% nonfat milk buffer and incubated with anti-p-p38, anti-p-p-c-Jun N-terminal kinase (JNK), anti-JNK (1:1,000, rabbit polyclonal antibodies; Bioworld Technology, St Louis Park, MN, USA), anti-p-38 (1:1,000, rabbit polyclonal antibodies; Cell Signaling Technology, Inc., Danvers, MA, USA), and anti-β-actin (1:1,000, a mouse polyclonal antibody), (Santa Cruz Biotechnology, Inc., Dallas, TX, USA) at 4°C overnight, washed with Tris-buffered saline and incubated with a horseradish peroxidase-conjugated antirabbit immunoglobulin (Ig)G/antimouse IgG secondary antibody at 37°C for 1 hour.
The second data set consisted of a total of 487 students including 217 boys and 270 girls in pubertal stage (aged 11.5-14.5 years) and 558 students including 382 boys and 276 girls in postpuberal stage (aged 14.5-18.5 years) which were randomly sampled by a multistage random sampling procedure from guidance and high schools of the four educational districts of Shiraz in the Spring of 2011.
The percentages of bone tissue in the apical region of the root, soft tissue (connective tissue and inflammatory infiltrate, when present), bone marrow and inferior alveolar nerve were obtained in a digital image analysis system, composed of a Zeiss Axioskop II microscope, CCD-IRIS RGB -Sony camera (Sony DXC-151A RGB Video Camera-Sony Corporation, Tokyo, Japan) and Kontron KS300 ® software (Kontron Electronic GMBM, Munich, Germany).
All data were assessed using the Statistical Package for the Social Sciences (SPSS) 15.0 program (SPSS Ver. 15.0,SPSS Inc, Chicago, IL, US) and were presented as mean± S.E.M. Differences between either the behavioral test or the counting of c-Fos-positive neurons were analyzed using a one-way analysis of variance (ANOVA) followed by Dunnett's post-hoc pair wise comparisons.
The following preprocessing procedures were performed using Statistical Parametric Mapping (SPM software (Wellcome Department of Imaging Neuroscience; London, UK) implemented in MATLAB R2009b (MathWorks; Natick, MA, USA) for whole brain analysis: correction for head motion, adjustment of acquisition timing across slices, spatial normalization using the MNI template, and smoothing using a Gaussian kernel with a full width at a half-maximum of 5 mm.
Indeed, we designed four 11-mer TALE-VP16 constructs for the same SCN9A bait sequence using the TALE-NT 2.0 program (Supplementary Table 3) and observed that all fusions, as well as combinations, failed to induce the overexpression of SCN9A mRNA levels in HEK293 (Fig. 2c, Supplementary Figs. 7 and 9).
The reasons for this can only be speculated -on the one hand, the TAppV and the related range of competency requirements is diverse, and not all day-one skills can be taught in a skills lab [7], [28]; on the other, veterinary studies in the past tended to focus exclusively on imparting knowledge, while practical education and skills training was normally carried out at the initiative of the universities themselves, using their own animals and patients in their own clinics [6], [29].
Among the model fits performed, logistic 4 P models also showed high R 2 values (R 2 5 0.9972 and BIC 5 19.93 for mortality rate; R 2 5 0.9997 and BIC 5 12.31 for abnormality rate) in addition to the power function models, but the complicated equations with relatively high BIC values discouraged the use of these equations for further analysis.
One-way ANOVA was used to compare the viral load in leaves infested by viruliferous B and Q. One-way ANOVA was also used to compare the JA content, PI activity, and relative gene expression of LOX and PI II in control leaves and leaves infested by nonviruliferous and viruliferous B and Q. SPSS version 20.0 (SPSS Inc., Chicago, IL, USA) was used for all statistical analyses.
Overrepresented functional categories include immunoglobulins, extracellular matrix, chitins and aminoglycans, immune response and wound healing, drug and hormone metabolism, chorion development, chemosensory response and development, and morphogenesis (supplementary table S (Wong and Wolfner 2012) and immune response to pathogens (Lazarro and Clark 2012) are known for their rapid evolution, and therefore these genes are strong candidates to search for evidence of ongoing selective sweeps.
Primer pairs were designed using Primer Premier 5.0 software (PREMIER Biosoft International, Palo Alto, California, USA) ( Wei et al., 2012 ) with the following criteria: primer lengths of 16-22 bp, GC content of 40-65%, annealing temperature ( T a ) ranging from 40 ° C to 60 ° C, and a predicted PCR product size ranging from 100 to 500 bp.
PCR amplifi cations were performed in 20-μ L volumes that included 50-80 ng of genomic DNA, 5 μ M concentrations of each primer, and 10 μ L 2 × PCRMaster Mix consisting of 0.1 unit/ μ L Taq DNA polymerase, 4 mM MgCl 2 , and 0.4 mM dNTP (Aidlab Co. Ltd., Beijing, China).
The PCR reactions were performed in a Veriti Dx 96-well Thermal Cycler (Applied Biosystems, Foster City, California, USA) under the following conditions: initial denaturation at 95 ° C for 5 min followed by 35 cycles at 95 ° C for 30 s, annealing for 45 s at the optimal temperature for each primer pair , and 72 ° C for 30 s, with a fi nal extension of 10 min at 72 ° C ( Table 1 ).
The images of 239 μm ×239 μmThe MBs at a concentration of ~1.7×10 6 bubbles/mL were injected via rat carotid artery at a constant speed of ~3 mL/min, the normal blood flow velocity at the carotid artery; after 10-15 seconds, the MBs reach the microvascular bed ~100-200 μm below the pia mater, 23 and the FUS sonication was administrated for 1 minute.
Following lysis of red blood cells (RBCs) with 2 mL cold isotonic solution (154 mM NH4Cl, 10 mM KHCO3 supplemented with 0.1 mM EDTA, pH 7.2), samples were centrifuged for 5 min at 300 × g at 4°C; cells were then washed once with phosphate buffered saline (PBS) before being resuspended in 300 μL of cold PBS and subsequently analyzed.
The data was analyzed using the EVOC (Ensemble de Programmes Permettant L´Analyse des Évocations) (Group of Programs for the Analysis of Evoked Responses) 2003 software, which allows one to achieve the organization of the words based on the hierarchy expressed through the frequency and the natural order of the evocation, through the construction of the Vergès matrix, where the evoked words are distributed, considering the criteria of higher frequencies and mean order of evocation (MOE)(6-7).
Thus, we calculated the emissions of these three gases separately as E(i) 5 AD * EF(i), where i is the gas type (CO 2 , CH 4 or N 2 O), AD is activity data and EF is the emission factor, 73.7 t/TJ for CO 2 37 and 0.0039 t/TJ for CH 4 and N 2 O 24 .
Because of the high daily mobility of these species (griffon vultures: up to 70 km from breeding sites; cinereous vultures: up to 86 km from breeding sites; bearded vultures: up to 45 km from breeding sites; Egyptian vultures: up to 70 km from breeding sites) 12,41, we also calculated their richness (i.e., number of species) in a 100 km buffer around each 10 3 10 km grid cell.
Separation of anthocyanins and non-anthocyanins was conducted on a reverse-phase Capcell Pak C18 UG 120 S5 column (4.6×150 mm I.D., 4 μm; Shiseido Co., Ltd, Tokyo, Japan) using a Finnigan Surveyor high-performance liquid chromatography (HPLC) system (Thermo Quest, San Jose, CA, USA) at an ambient temperature.
The mobile phase was a binary elution of 5% formic acid solution (A) and 100% methanol (B) under the following gradient conditions: initial, 20% B; 7 min, 20% B; 15 min, 25% B; 32 min, 50% B; 36 min, 100% B; 38 min, 100% B; 40 min, 20% B and 45 min, 20% B. The flow rate was 700 μl/min, and the injection volume was 10 μl.
The membranes were immunoblotted with a primary antibody against human statherin (1:2000 dilution; STATH Polyclonal Antibody [catalog #19724-1-AP], Proteintech, IL, USA) at 4°C overnight, followed by incubation with horseradish peroxidase-labeled secondary antibody (1:10,000) (GE Healthcare Life Sciences, PA, USA).
The following pre-processing was applied: motion correction using MCFLIRT [Motion Correction: FMRIB3s Linear Image Registration Tool (Jenkinson et al., 2002)], slice-timing correction using Fourier-space time-series phase-shifting; non-brain removal using the Brain Extraction Tool (Smith, 2002), spatial smoothing using a Gaussian kernel of FWHM 6.0 mm; high-pass temporal filtering (Gaussian-weighted least-squares straight line fitting, with sigma = 50.0 s).
To account for the increased probability of Type 1 error through the use of multiple HRFs, data were corrected using AlphaSim of the AFNI analysis packagehttp://afni.nimh.nih.gov/pub/dist/doc/program_help/AlphaSim.html that performs both probability and cluster-based thresholding at a significance level of p b 0.05, as determined by Monte Carlo simulations (Ward, 2000).
Of note, when FAC sorting of ISCs is performed for the first time, the parameters for sorting and for compensation need to be set using the aforementioned controls: (1) dissociated cells from wild type (e.g.Of note, the advantage of using Sytox instead of propidium iodide to label dead cells is that there is only a low spillover into the FITC (GFP) channel, which makes it easy to compensate for the spillover.
Dragon Descriptors-The following types of descriptors were generated using Dragon software (v.5.5, Talete SRL, Milan, Italy): 0D constitutional (atom and group counts), 1D functional groups, 1D atom-centered fragments, 2D topological descriptors, 2D walk and path counts, 2D autocorrelations, 2D connectivity indices, 2D information indices, 2D topological charge indices, 2D eigenvalue-based indices, 2D topological descriptors, 2D edge-adjacency indices, 2D Burden eigenvalues, 2D binary fingerprints, 2D frequency fingerprints, and molecular properties.
Comparison of the developed consensus model with DERMWIN software showed that our model significantly outperformed DERMWIN in predictivity (Q 2 ext = 72% and 43%, respectively) but at the expense of some lost in coverage (77% vs. 100%); when applied to the same set of compounds as used for DERMWIN (ignoring the AD for our models) the performance of our model is still higher (Q 2 ext = 71% vs. 66%).
The synovium-infiltrating cells (SIC) from arthritic Lewis rats treated with the vehicle (control) or Celastrol (test) were cultured in RPMI 1640 (Quality Biologics) supplemented with 10% fetal bovine serum (FBS), 1% L-glutamine, 1% penicillin/ streptomycin (all from Invitrogen), and 0.1% β-mercaptoethanol (Sigma).
The process is summarized as: (1) the data are standardized such that each variable has mean 0 and norm 1; (2) a grid search of two tuning parameters associated with each penalty function is applied to the model (one for the fixed effects and one for the random effects), which control the strength of the shrinkage; (3) the model that gives a minimum Bayesian Information Criteria [47] is chosen as the optimal model that gives the best balance of fit and parsimony; and (4) the model is refit with only the selected fixed and random effects, with no constraints applied to the coefficients, using PROC GLIMMIX in SAS (SAS Institute, Carey, NC).
Adult mice were deeply anesthetized and transcardially perfused with chilled (4 C) artificial cerebrospinal fluid (ACSF) (119 mM NaCl, 2.5 mM KCl, 26 mM NaHCO 3 , 1 mM NaH 2 PO 4 , 11 mM D-glucose at pH 7.4, 300 mOsm, and aerated with 95% O 2 /5% CO 2 ), including 0.5 mM CaCl 2 , 5 mM MgCl 2 , and 1% kynurenate acid.
Whole-cell currentclamp and voltage-clamp recordings were performed using glass pipettes ($6-9 MU) filled with intracellular solution (0.2 mM EGTA, 130 mM K-gluconate, 6 mM KCl, 3 mM NaCl, 10 mM HEPES, 4 mM ATP-Mg, 0.4 mM GTP-Na, and 14 mM phosphocreatine-Tris at pH 7.2 and 285 mOsm).
The fractions of isolated mitochondria obtained (mixed subsarcolemmal and intermyofibrillar) were resuspended in a small volume of MIM þ BSA and frozen at À80 for biochemical analysis.Membranes were incubated using infrared fluorophore-conjugated secondary antibodies (LiCor Biosciences, Lincoln, NE), scanned using Odyssey Clx Infrared Imaging System (Li-Cor) and analyzed by densitometry using Image J (NIH).
Samples were acquired on a FACSCalibur flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA) and the data were analyzed by FlowJo software.protein were incubated at 100°C for 5 minutes, separated by 10% (w/v) sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and electrophoretically transferred onto a polyvinylidene difluoride transfer membrane (Bio-Rad Laboratories, Hercules, CA, USA).
The membrane was incubated with antibodies against β-actin (Sigma-Aldrich), IκBα (44D4), phospho-IκBα (pIκBα) (Ser32/36, 5A5), signal transducer and activator of transcription 1 (STAT1), and pSTAT1 (Ser727; Cell Signaling Technology, Danvers, MA, USA) in TBST containing 5% bovine serum albumin overnight at 4°C, then with horseradish peroxidase-conjugated second antibodies (anti-rabbit IgG [Sigma-Aldrich] and anti-mouse immunoglobulin G [Cell Signaling Technology], at a dilution of 1/5,000 and 1/2,000, respectively).
Because both sulfide oxidation and dissimilatory sulfate reduction pathways are mediated by the same set of genes (aprA, aprB and dsrA) but are found in different families of bacteria, we assigned metagenomic reads to each pathway according to phylogeny, that is, sulfate reduction for Firmicutes and Deltaproteobacteria reads, and sulfide oxidation for Alphaproteobacteria, Betaproteobacteria, Chlorobiaceae and Chromatiaceae.
Available mRNA data for these COL genes indicated that they showed an expression pattern similar to COL1/2 of A. thaliana (Hecht et al. 2005, 2007; Bohlenius et al. 2006; Almada et al. 2009; Gonz alez- Schain et al. 2012; Hsu et al. 2012; Kloosterman et al. 2013).
CO Promoters from Capsella rubella, Nasturtium officinale, and Sisymbrium officinale were amplified from genomic DNA (kindly provided by Markus Berns, MPIPZ, Cologne) using degenerate primers that anchor in COL1 and CO, respectively (COL1 Fw: TGACACMGGATATG GAATTG; CO Rv: TGGCAGAGTGRACTTGAGCA).
BH-T2WI = breath-hold multi-shot T2-weighted image, RT-T2WI = respiratory-triggered multi-shot T2-weighted image, SD = standard deviationkjronline.org the subjective assignment of anatomic sharpness of upper abdominal organ margin was highest in MultiVane T2WI (4.95), followed by BH-T2WI (4.65) and RT-T2WI (4.33), with a significant difference among the sequences (p < 0.001).
Rxy(τ)=1Nt−1∑tNt−τ(x(t+τ)−x¯)(y(t)−y¯)σxσy(1)The linear trend of time series signals (x(t) and y(t)) was excluded using the “detrend” function of MATLAB (The MathWorks Inc, USA) and the signals were filtered using Hanning windows of the same size as the data length before their cross correlation was calculated (the MATLAB “detrend” function computes the least-squares fit of a straight line to the data in question and subtracts the resulting function from the data).
The following factors were investigated: PM 10 , SO 2 , NO 2 , CO, O 3 , PM 2.5 , age, PD duration, serum Cr level, white blood cell count (WBC), nPNA, serum albumin, serum total IS, serum total PCS, residual renal function test, intact parathyroid hormone level (iPTH), blood aluminum (Al) level, diabetes mellitus (DM), coronary artery disease (CAD), and hypertension.
For wide-field fluorescence microscopy, yeast cells were imaged on an agarose pad containing 50 mM potassium phosphate buffer, pH 7, and images were collected at room temperature using a 1.49 NA 100× objective on a Ti-E inverted microscope equipped with a Ti-S-E motorized stage (Nikon), piezo Z-control (Physik Instrumente), a SOLA SM II LE LED light engine (Lumencor), a motorized filter cube turret, and an iXon X3 DU897 cooled EM-CCD camera (Andor).
The migration and invasion abilities of human HCC cells transfected with miR-328/NC mimic or miR-328/NC inhibitor with or without si-PTPRJ/si-con were tested by migration and invasion assays, respectively, using the 24-well cell migration kit with 8 µm-pore polycarbonate membrane (Corning Inc, Corning, NY, USA) and the invasion assay kit (BD Bioscience, San Jose, CA, USA), according to the manufacturer's instructions.
3,17,18 The cut-offs for dichotomization were taken as 5.88 mg/dl for serum uric acid, 19 0.8 mg/dl for serum creatinine 16 and 1.3 mg/L for serum cystatin c. 16 Serum uric acid ≥ 5.88 mg/dl, serum creatinine ≥ 0.8 mg/dl and serum cystatin C ≥ 1.3 mg/L were coded as 1 and those less than the cut-offs were coded as 0.
The possible confounders were age, BMI, gestational age, parity and birth weight in the first analysis where hypertensive disorders were taken as the dependent variable (Table 2); age, BMI, gestational age, parity and hypertensive disorders in the second analysis where birth weight was taken as the dependent variable (Table 3); age, BMI, parity, birth weight and hypertensive disorders in the third analysis where the gestational age of delivery is the outcome Table 4); age, BMI, parity, birth weight, gestational age and hypertensive disorders in the fourth analysis where mode of delivery was taken as the dependent variable (Table 5).
To determine the relative radial positioning of a gene in a given tissue, 91-263 randomly selected interphase epithelial nuclei were run though a custom-made image analysis software package (P. Gudla and S. Lockett, National Cancer Institute, Bethesda, MD; Meaburn et al., 2009) in MATLAB (MathWorks, Natick, MA) using the PRTools and DIPImage toolboxes (Delft University of Technology, Delft, Netherlands).
Totally, 28 covariates were employed in the multivariate model, including diabetes, previous PCI, medina classification, plaque location,reference diameter of the proximal and distal MV, reference diameter of the SB, preprocedural percent diameter stenosis of the proximal MV, preprocedural percent diameter stenosis of the distal MV, true bifurcation lesions, lesion length of the MV and SB, bifurcation angle, predilation of the SB and jailed wire in SB.
The C max and T max of 5-FU solid microspheres and powder were 2.25±0.22 mg/L and 1.00±0.15 hours, and 2.55±0.19 mg/L and 0.75±0.11 hours, respectively; the plasma drug concentration was detectable up to 12 and 8 hours, whereas theC max and T max of hollow microspheres were 1.64±0.21 mg/L and 2.00±0.22 hours, respectively.
To validate if miR-381 directly targets Twist1 3′UTR (Figure 3A), luciferase reporter assays were performed in HT29 and SW480 cells, which revealed that miR-381 mimics significantly decreased the luciferase activity of wild-type Twist1 3′UTR reporter, but had no effect on the Twist1 3′UTR reporter with mutant miR-381 binding seed sites (Figure 3B).
The amplification was carried out using a Touchdown program for all microsatellite loci, which consists of initial denaturation of 95°C for 1 min; 3 cycles of 95°C for 45 s and 60°C for 1 min, 3 cycle of 95°C for 45 s and 57°C for 1 min; 3 cycles of 95°C for 45 s and 54°C for 1 min, 3 cycles of 95°C for 45 s and 51°C for 1 min and 20 cycles of 95°C for 45 s and 48°C for 1 min.
The raw ChIP-seq data set (18), which contains three input samples (Swi6, Tup1 and RNA polymerase II phosphorylated at serine 5 (Pol2ser5p)) and a mock immunoprecipitation (IP) control sample (DMSO, Illumina), was obtained from GEO (data set GSE51251; http://www.ncbi. nlm.nih.gov/geo/query/acc.cgi?acc = GSE51251).
We filtered the sequencing reads (using the R package 'ShortRead' (29) version 1.20.0 with a custom filter) to retain only those with two or less expected errors per read: given a Phred quality score q for each base call, the probability that a base call is erroneous is P = 10 (− q / 10) , which is then summed over the bases in the read to give the expected errors per read.
After the establishment of the long-term everolimus-treated BON-1/R and QGP-1/R, both showed a statistically significant reduced growth inhibitory response to everolimus in comparison with longterm vehicle-treated BON-1 and QGP-1 at everolimus concentrations between 10 nM and 1 mM for BON-1/R and at 1 nM and 1 mM for QGP-1/R, respectively Figure 2A and B).
RNA was isolated using the Aurum Total RNA Mini Kit which incorporates an on-column genomic DNA clearance step (Cat# 7326820, Bio-Rad Laboratories, Hercules, CA), quantified with a NanoDrop ND-1000 spectrophotometer (Thermo Fischer Scientific, Waltham, MA) and stored at −80 • C. ERCC ExFold RNA Spike-in Mixes (Cat# 4456739, Thermo Fischer Scientific, Waltham, MA) were diluted and spiked into RNA samples which were then reverse transcribed using iScript Reverse Transcription Supermix as directed by the manufacturer (Cat# 1708849, Bio-Rad Laboratories, Hercules, CA) to make stock cDNA samples which were stored at −20 • C. Stock cDNA samples were pre-amplified for 10-20 cycles using SsoAdvanced PreAmp Supermix as directed by the manufacturer (Cat# 1725160, Bio-Rad Laboratories, Hercules, CA) diluted at least 5-fold and stored at −20 • C. PreAmp was performed with panels of assays which include PrimePCR PreAmp assays (Bio-Rad Laboratories, Hercules, CA), assays culled from public databases, assays designed using online software tools and assays from published manuscripts (Supplemental Fig. 1); primers were at a final concentration of 50 nM each.
Cells were incubated in triplicate with PBS (control), 20 μg/ml recombinant human PDCD5 (rhPDCD5; P20; Beijing University Human Disease Center, Beijing, China), 0.1 μg/ml taxol (T0.1), 0.5 μg/ml doxorubicin (A0.5), or P20 plus T0.1 or A0.5.