Ewing sarcoma

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Note: certain regimens have been moved to dedicated pages:
*Pediatric Ewing sarcoma
Last updated on 2024-07-23:22 regimens on this page
36 variants on this page
=Guidelines=
Given the rapid change in evidence in many areas of hematology/oncology, readers are encouraged to consider any guideline published 5+ years ago to be for historical purposes, only.
==ESMO==
*2009: Ewing's sarcoma of the bone: ESMO clinical recommendations for diagnosis, treatment and follow-up EuroPMC
**2008: Paulussen et al. Ewing's sarcoma of the bone: ESMO clinical recommendations for diagnosis, treatment and follow-up EuroPMC
**2007: Saeter. Ewing's sarcoma of bone: ESMO clinical recommendations for diagnosis, treatment and follow-up EuroPMC
**2005: Saeter et al. ESMO Minimum Clinical Recommendations for diagnosis, treatment and follow-up of Ewing's sarcoma of bone EuroPMC
**2003: Saeter. ESMO Minimum Clinical Recommendations for diagnosis, treatment and follow-up of Ewing's sarcoma of bone EuroPMC
==NCCN==
*NCCN does not currently have guidelines at this granular level; please see NCCN Guidelines - Bone Cancer.
=Neoadjuvant therapy=
==EVAIA==
EVAIA: Etoposide, Vincristine, Adriamycin (Doxorubicin), Ifosfamide, DActinomycin
===Regimen===
Study 	Dates of enrollment	Evidence 	Comparator	Comparative Efficacy
Paulussen et al. 2008 (EICESS-92)	1992-1997 	Phase 3 (E-esc)	VAIA 	Did not meet primary endpoint of EFS36
Note: This regimen is intended for high-risk patients.
====Chemotherapy====
*Etoposide (Vepesid) 150 mg/m2 IV once per day on days 1 to 3
*Vincristine (Oncovin) 1.5 mg/m2 IV once on day 1
*Doxorubicin (Adriamycin) 30 mg/m2 IV once per day on days 2 & 4
*Ifosfamide (Ifex) 2000 mg/m2 IV once per day on days 1 to 3
**Note: primary reference does not comment about the use of mesna
*Dactinomycin (Cosmegen) 0.5 mg/m2 IV once per day on days 1, 3, 5
21-day cycle for 4 cycles
====Subsequent treatment====
*Surgical removal of tumors is done when possible.
*EICESS-92, patients not undergoing surgery, with incomplete surgical resection, or poor histologic response: Definitive external beam radiotherapy x 5440 cGy, then adjuvant EVAIA
*EICESS-92, patients with a good histologic response: Adjuvant External beam radiotherapy 4480 cGy, then adjuvant EVAIA
*Additional details about particular clinical scenarios can be found in the original reference
===References===
# EICESS-92: Paulussen M, Craft AW, Lewis I, Hackshaw A, Douglas C, Dunst J, Schuck A, Winkelmann W, Köhler G, Poremba C, Zoubek A, Ladenstein R, van den Berg H, Hunold A, Cassoni A, Spooner D, Grimer R, Whelan J, McTiernan A, Jürgens H; European Intergroup Cooperative Ewing's Sarcoma Study-92. Results of the EICESS-92 Study: two randomized trials of Ewing's sarcoma treatment--cyclophosphamide compared with ifosfamide in standard-risk patients and assessment of benefit of etoposide added to standard treatment in high-risk patients. J Clin Oncol. 2008 Sep 20;26(27):4385-93. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC NCT00002516
==VACA==
VACA: Vincristine, Adriamycin (Doxorubicin), Cyclophosphamide, DActinomycin
===Regimen variant #1===
Study 	Dates of enrollment	Evidence 	Comparator	Comparative Efficacy
Grier et al. 2003 (INT-0091)	1988-1992 	Phase 3 (C)	VACA/IE 	Shorter OS
Note: The survival disadvantage in Grier et al. 2003 was only noted for patients with non-metastatic disease at diagnosis.
====Chemotherapy====
*Vincristine (Oncovin) 2 mg/m2 (maximum dose of 2 mg) IV once on day 1
*Doxorubicin (Adriamycin) 75 mg/m2 IV bolus once on day 1
**Stop once cumulative dose received by the patient exceeds 375 mg/m2
*Cyclophosphamide (Cytoxan) 1200 mg/m2 IV once on day 1
*Dactinomycin (Cosmegen) 1.25 mg/m2 IV once on day 1, once cumulative doxorubicin dose received by the patient exceeds 375 mg/m2
====Supportive therapy====
*Mesna (Mesnex) after cyclophosphamide for prevention of hemorrhagic cystitis; primary reference did not list dosage/schedule
21-day cycle for 17 cycles
====Subsequent treatment====
*Definitive local therapy is planned to take place on week 12
*Surgery can be performed for resectable tumors. No radiation therapy is given for completely resected primary tumors with negative margins.
**INT-0091, residual tumor after surgery: 4500 cGy radiation is administered to the original tumor volume plus a 1 cm margin 
*If only radiation therapy is used, 4500 cGy of radiation is administered to the tumor volume plus a 3 cm margin, followed by 1080 cGy to only the preradiation tumor volume, for a total dose of 5580 cGy
===Regimen variant #2===
Study 	Dates of enrollment	Evidence
Paulussen et al. 2001 (CESS 86)	1986-01 to 1991-07 	Non-randomized
Note: This regimen is intended for standard risk patients.
====Chemotherapy====
*Vincristine (Oncovin) 1.5 mg/m2 IV once per day on days 1, 8, 15, 22
*Doxorubicin (Adriamycin) 30 mg/m2 IV once per day on days 1, 2, 43, 44
*Cyclophosphamide (Cytoxan) 1200 mg/m2 IV once on day 1, then 400 mg/m2 IV once per day on days 22, 23, 24, then 1200 mg/m2 IV once on day 43
*Dactinomycin (Cosmegen) 0.5 mg/m2 IV once per day on days 22, 23, 24
====Supportive therapy====
*Mesna (Mesnex) "as appropriate"
9-week "block", then proceed to local therapy:
====Local therapy====
*Complete surgical removal of tumors is done when possible.
*CESS 86, patients not undergoing surgery: External beam radiotherapy 6000 cGy to the tumor bulk, with the tumor-bearing compartment receiving at least 4480 cGy
*CESS 86, patients with incomplete surgical resection or poor histologic response: External beam radiotherapy 4480 cGy
====Subsequent treatment====
*Adjuvant VACA
===References===
# CESS 86: Paulussen M, Ahrens S, Dunst J, Winkelmann W, Exner GU, Kotz R, Amann G, Dockhorn-Dworniczak B, Harms D, Müller-Weihrich S, Welte K, Kornhuber B, Janka-Schaub G, Göbel U, Treuner J, Voûte PA, Zoubek A, Gadner H, Jürgens H. Localized Ewing tumor of bone: final results of the cooperative Ewing's Sarcoma Study CESS 86. J Clin Oncol. 2001 Mar 15;19(6):1818-29. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC
# INT-0091: Grier HE, Krailo MD, Tarbell NJ, Link MP, Fryer CJ, Pritchard DJ, Gebhardt MC, Dickman PS, Perlman EJ, Meyers PA, Donaldson SS, Moore S, Rausen AR, Vietti TJ, Miser JS; CCG; POG. Addition of ifosfamide and etoposide to standard chemotherapy for Ewing's sarcoma and primitive neuroectodermal tumor of bone. N Engl J Med. 2003 Feb 20;348(8):694-701. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC
==VACA/IE==
VACA/IE: Vincristine, Adriamycin (Doxorubicin), Cyclophosphamide, DActinomycin alternating with Ifosfamide, Etoposide
Study 	Dates of enrollment	Evidence 	Comparator	Comparative Efficacy
Grier et al. 2003 (INT-0091)	1988-1992 	Phase 3 (E-esc)	VACA 	Longer OS1
1The survival advantage in Grier et al. 2003 was only noted for patients with non-metastatic disease at diagnosis. The dosing schedule below assumes that the patient is doxorubicin-naive.
===Induction===
====Chemotherapy, VACA portion (cycles 1 & 3)====
*Vincristine (Oncovin) 2 mg/m2 (maximum dose of 2 mg) IV once on day 1
*Doxorubicin (Adriamycin) 75 mg/m2 IV bolus once on day 1
*Cyclophosphamide (Cytoxan) 1200 mg/m2 IV once on day 1
====Supportive therapy, VACA portion (cycles 1 & 3)====
*Mesna (Mesnex) after cyclophosphamide for prevention of hemorrhagic cystitis; primary reference did not list dosage/schedule
====Chemotherapy, IE portion (cycles 2 & 4)====
*Ifosfamide (Ifex) 1800 mg/m2 IV once per day on days 1 to 5, with mesa
*Etoposide (Vepesid) 100 mg/m2 IV once per day on days 1 to 5
====Supportive therapy, IE portion (cycles 2 & 4)====
*Mesna (Mesnex) with ifosfamide; primary reference did not list dosage/schedule
21-day cycle for 4 cycles, followed by:
===Definitive===
Local therapy is planned to take place on week 12, as follows:
*Surgery can be performed for resectable tumors. No radiation therapy is given for completely resected primary tumors with negative margins.
**INT-0091, residual tumor after surgery: 4500 cGy radiation is administered to the original tumor volume plus a 1 cm margin 
*If only radiation therapy is used, 4500 cGy of radiation is administered to the tumor volume plus a 3 cm margin, followed by 1080 cGy to only the preradiation tumor volume, for a total dose of 5580 cGy
===Consolidation===
====Chemotherapy, VACA portion====
*Vincristine (Oncovin) as follows:
**Cycles 5, 7, 9, 11, 13: 2 mg/m2 (maximum dose of 2 mg) IV once on day 1
*Doxorubicin (Adriamycin) as follows:
**Cycle 5: 75 mg/m2 IV bolus once on day 1
**Stop once cumulative dose received by the patient exceeds 375 mg/m2
*Cyclophosphamide (Cytoxan) as follows:
**Cycles 5, 7, 9, 11, 13: 1200 mg/m2 IV once on day 1
*Dactinomycin (Cosmegen) as follows:
**Cycles 7, 9, 11, 13: 1.25 mg/m2 IV once on day 1, once cumulative doxorubicin dose received by the patient exceeds 375 mg/m2
====Supportive therapy, VACA portion (cycles 5, 7, 9, 11, 13)====
*Mesna (Mesnex) after cyclophosphamide for prevention of hemorrhagic cystitis; primary reference did not list dosage/schedule
====Chemotherapy, IE portion (cycles 6, 8, 10, 12)====
*Ifosfamide (Ifex) 1800 mg/m2 IV once per day on days 1 to 5, with mesna
*Etoposide (Vepesid) 100 mg/m2 IV once per day on days 1 to 5
====Supportive therapy, IE portion (cycles 6, 8, 10, 12)====
*Mesna (Mesnex) with ifosfamide; primary reference did not list dosage/schedule
21-day cycle for 13 cycles (17 total cycles of chemotherapy)
===References===
# INT-0091: Grier HE, Krailo MD, Tarbell NJ, Link MP, Fryer CJ, Pritchard DJ, Gebhardt MC, Dickman PS, Perlman EJ, Meyers PA, Donaldson SS, Moore S, Rausen AR, Vietti TJ, Miser JS. Addition of ifosfamide and etoposide to standard chemotherapy for Ewing's sarcoma and primitive neuroectodermal tumor of bone. N Engl J Med. 2003 Feb 20;348(8):694-701. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC
==VAIA==
VAIA: Vincristine, Adriamycin (Doxorubicin), Ifosfamide, Actinomycin-D (Dactinomycin)
===Regimen variant #1===
Study 	Dates of enrollment	Evidence 	Comparator 	Comparative Efficacy
Paulussen et al. 2008 (EICESS-92)	1992-1997 	Phase 3 (C)	EVAIA (high-risk)	Did not meet primary endpoint of EFS36
Note: high-risk patients were randomized to this regimen versus EVAIA. Standard-risk patients were not randomized at this point of the protocol.
====Chemotherapy====
*Vincristine (Oncovin) 1.5 mg/m2 IV once on day 1
*Doxorubicin (Adriamycin) 30 mg/m2 IV once per day on days 2 & 4
*Ifosfamide (Ifex) 2000 mg/m2 IV once per day on days 1 to 3
**Note: primary reference does not comment about the use of mesna
*Dactinomycin (Cosmegen) 0.5 mg/m2 IV once per day on days 1, 3, 5
21-day cycle for 4 cycles, then proceed to local therapy:
====Local therapy====
*Surgical removal of tumors is done when possible.
*EICESS-92, patients not undergoing surgery, with incomplete surgical resection, or poor histologic response: External beam radiotherapy 5440 cGy
*EICESS-92, patients with a good histologic response: External beam radiotherapy 4480 cGy
*Additional details about particular clinical scenarios can be found in the original reference
====Subsequent treatment====
*EICESS-92, high-risk patients: Adjuvant VAIA
*EICESS-92, standard-risk patients: Adjuvant VAIA versus VACA
===Regimen variant #2===
Study 	Dates of enrollment	Evidence
Paulussen et al. 2001 (CESS 86)	1986-01 to 1991-07 	Non-randomized
Note: This regimen is intended for high risk patients.
====Chemotherapy====
*Vincristine (Oncovin) 1.5 mg/m2 IV once per day on days 1, 8, 15, 22
*Doxorubicin (Adriamycin) 30 mg/m2 IV once per day on days 1, 2, 43, 44
*Ifosfamide (Ifex) 3000 mg/m2 IV once per day on days 1, 2, 22, 23, 43, 44
*Dactinomycin (Cosmegen) 0.5 mg/m2 IV once per day on days 22, 23, 24
====Supportive therapy====
*Mesna (Mesnex) "as appropriate"
9-week "block", then proceed to local therapy:
====Local therapy====
*Complete surgical removal of tumors is done when possible.
*CESS 86, patients not undergoing surgery: External beam radiotherapy 6000 cGy to the tumor bulk, with the tumor-bearing compartment receiving at least 4480 cGy
*CESS 86, patients with incomplete surgical resection or poor histologic response: External beam radiotherapy 4480 cGy 
====Subsequent treatment====
*Adjuvant VAIA
===References===
# CESS 86: Paulussen M, Ahrens S, Dunst J, Winkelmann W, Exner GU, Kotz R, Amann G, Dockhorn-Dworniczak B, Harms D, Müller-Weihrich S, Welte K, Kornhuber B, Janka-Schaub G, Göbel U, Treuner J, Voûte PA, Zoubek A, Gadner H, Jürgens H. Localized Ewing tumor of bone: final results of the cooperative Ewing's Sarcoma Study CESS 86. J Clin Oncol. 2001 Mar 15;19(6):1818-29. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC
# EICESS-92: Paulussen M, Craft AW, Lewis I, Hackshaw A, Douglas C, Dunst J, Schuck A, Winkelmann W, Köhler G, Poremba C, Zoubek A, Ladenstein R, van den Berg H, Hunold A, Cassoni A, Spooner D, Grimer R, Whelan J, McTiernan A, Jürgens H; European Intergroup Cooperative Ewing's Sarcoma Study-92. Results of the EICESS-92 Study: two randomized trials of Ewing's sarcoma treatment--cyclophosphamide compared with ifosfamide in standard-risk patients and assessment of benefit of etoposide added to standard treatment in high-risk patients. J Clin Oncol. 2008 Sep 20;26(27):4385-93. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC NCT00002516
#CWS/RMS-96: Sparber-Sauer M, Ferrari A, Kosztyla D, Ladenstein R, Cecchetto G, Kazanowska B, Scarzello G, Ljungman G, Milano GM, Niggli F, Alaggio R, Vokuhl C, Casanova M, Klingebiel T, Zin A, Koscielniak E, Bisogno G. Long-term results from the multicentric European randomized phase 3 trial CWS/RMS-96 for localized high-risk soft tissue sarcoma in children, adolescents, and young adults. Pediatr Blood Cancer. 2022 Sep;69(9):e29691. Epub 2022 Apr 19. link to original article EuroPMC
##Update: Sparber-Sauer M, Ferrari A, Kosztyla D, Ladenstein R, Cecchetto G, Kazanowska B, Scarzello G, Ljungman G, Milano GM, Niggli F, Alaggio R, Vokuhl C, Casanova M, Klingebiel T, Zin A, Koscielniak E, Bisogno G. Long-term results from the multicentric European randomized phase 3 trial CWS/RMS-96 for localized high-risk soft tissue sarcoma in children, adolescents, and young adults. Pediatr Blood Cancer. 2022 Sep;69(9):e29691. Epub 2022 Apr 19. link to original article EuroPMC
==VDC/IE==
VDC/IE: Vincristine, Doxorubicin, Cyclophosphamide, alternating with Ifosfamide & Etoposide
VAdriaC/IE: Vincristine, Adriamycin (Doxorubicin), Cyclophosphamide, alternating with Ifosfamide & Etoposide
===Regimen variant #1, q2wk===
Study 	Dates of enrollment 	Evidence 	Comparator 	Comparative Efficacy
Womer et al. 2012 (COG AEWS0031) 	2001-05 to 2005-08 	Phase 3 (E-esc) 	VDC/IE; standard 	Seems to have longer EFS (primary endpoint)EFS60: 73% vs 65%(HR 0.74, 95% CI 0.54-0.99)
Brennan et al. 2022 (EE2012) 	2014-03-21 to 2019-05-01	Phase 3 (E-de-esc)	VIDE 	Longer EFS (primary endpoint)EFS36: 67% vs 61%(aHR 0.71, 95% CI 0.55-0.92)
DuBois et al. 2023 (COG AEWS1221)	2014-12 to 2019-03 	Phase 3 (C) 	VDC/IE & Ganitumab	Did not meet primary endpoint of EFSEFS36: 37.4% vs 39.1%(sHR 1.00, 95% CI 0.75-1.32)
====Chemotherapy, VDC portion (cycles 1, 3, 5)====
*Vincristine (Oncovin) 1.5 mg/m2 (maximum dose of 2 mg) IV once on day 1
*Doxorubicin (Adriamycin) 37.5 mg/m2 IV once per day on days 1 & 2
*Cyclophosphamide (Cytoxan) 1200 mg/m2 IV once on day 1
====Supportive therapy, VDC portion (cycles 1, 3, 5)====
*Mesna (Mesnex) with cyclophosphamide (dose/schedule not specified)
*Filgrastim (Neupogen) schedule not specified
====Chemotherapy, IE portion (cycles 2, 4, 6)====
*Ifosfamide (Ifex) 1800 mg/m2 IV once per day on days 1 to 5, with mesna
*Etoposide (Vepesid) 100 mg/m2 IV once per day on days 1 to 5
====Supportive therapy, IE portion (cycles 2, 4, 6)====
*Mesna (Mesnex) with ifosfamide; primary reference did not list dosage/schedule
*Filgrastim (Neupogen) schedule not specified
14-day cycle for 6 cycles (VDC/IE x 3)
====Subsequent treatment====
*Definitive local therapy, then adjuvant VDC/IE
===Regimen variant #2, q2wk with extra vincristine===
Study 	Dates of enrollment 	Evidence 	Comparator	Comparative Efficacy
Leavey et al. 2021 (COG AEWS1031)	2010-11-22 to 2016-01-04	Phase 3 (C)	VDC/IE/VTC	Did not meet primary endpoint of EFS
Note: the only difference between this and the variant above is the additional dose of vincristine in the second week of each VDC cycle.
====Chemotherapy, VDC portion (cycles 1, 3, 5)====
*Vincristine (Oncovin) 1.5 mg/m2 (maximum dose of 2 mg) IV once per day on days 1 & 8
*Doxorubicin (Adriamycin) 37.5 mg/m2 IV once per day on days 1 & 2
*Cyclophosphamide (Cytoxan) 1200 mg/m2 IV once on day 1
====Chemotherapy, IE portion (cycles 2, 4, 6)====
*Ifosfamide (Ifex) 1800 mg/m2 IV once per day on days 1 to 5
*Etoposide (Vepesid) 100 mg/m2 IV once per day on days 1 to 5
====Supportive therapy, both portions (cycles 1 to 6)====
*Filgrastim (Neupogen) details not specified
14-day cycle for 6 cycles (VDC/IE x 3)
====Subsequent treatment====
*Definitive local therapy, then VDC/IE continuation
===Regimen variant #3, q3wk===
Study 	Dates of enrollment	Evidence 	Comparator 	Comparative Efficacy
Womer et al. 2012 (COG AEWS0031)	2001-05 to 2005-08 	Phase 3 (C)	VDC/IE; dose-intense	Seems to have shorter EFS (primary endpoint)EFS60: 65% vs 73%(HR 1.35, 95% CI 1.01-1.85)
====Chemotherapy, VDC portion (cycles 1 & 3)====
*Vincristine (Oncovin) 1.5 mg/m2 (maximum dose of 2 mg) IV once on day 1
*Doxorubicin (Adriamycin) 37.5 mg/m2 IV once per day on days 1 & 2
*Cyclophosphamide (Cytoxan) 1200 mg/m2 IV once on day 1
====Supportive therapy, VDC portion (cycles 1 & 3)====
*Mesna (Mesnex) with cyclophosphamide; primary reference did not list dosage/schedule
*Filgrastim (Neupogen)
====Chemotherapy, IE portion (cycles 2 & 4)====
*Ifosfamide (Ifex) 1800 mg/m2 IV once per day on days 1 to 5, with mesna
*Etoposide (Vepesid) 100 mg/m2 IV once per day on days 1 to 5
====Supportive therapy, IE portion (cycles 2 & 4)====
*Mesna (Mesnex) with ifosfamide; primary reference did not list dosage/schedule
*Filgrastim (Neupogen)
21-day cycle for 4 cycles
====Subsequent treatment====
*Definitive local therapy, then adjuvant VDC/IE
===References===
# COG AEWS0031: Womer RB, West DC, Krailo MD, Dickman PS, Pawel BR, Grier HE, Marcus K, Sailer S, Healey JH, Dormans JP, Weiss AR; Children's Oncology Group. Randomized controlled trial of interval-compressed chemotherapy for the treatment of localized Ewing sarcoma: a report from the Children's Oncology Group. J Clin Oncol. 2012 Nov 20;30(33):4148-54. Epub 2012 Oct 22. Erratum in: J Clin Oncol. 2015 Mar 1;33(7):814. Dosage error in article text. link to original article link to PMC article dosing details in manuscript have been reviewed by our editors EuroPMC NCT00006734
##Update: Cash T, Krailo MD, Buxton AB, Pawel BR, Healey JH, Binitie O, Marcus KJ, Grier HE, Grohar PJ, Reed DR, Weiss AR, Gorlick R, Janeway KA, DuBois SG, Womer RB. Long-Term Outcomes in Patients With Localized Ewing Sarcoma Treated With Interval-Compressed Chemotherapy on Children's Oncology Group Study AEWS0031. J Clin Oncol. 2023 Oct 20;41(30):4724-4728. Epub 2023 Aug 31. link to original article link to PMC article EuroPMC
# COG AEWS1031: Leavey PJ, Laack NN, Krailo MD, Buxton A, Randall RL, DuBois SG, Reed DR, Grier HE, Hawkins DS, Pawel B, Nadel H, Womer RB, Letson GD, Bernstein M, Brown K, Maciej A, Chuba P, Ahmed AA, Indelicato DJ, Wang D, Marina N, Gorlick R, Janeway KA, Mascarenhas L. Phase III Trial Adding Vincristine-Topotecan-Cyclophosphamide to the Initial Treatment of Patients With Nonmetastatic Ewing Sarcoma: A Children's Oncology Group Report. J Clin Oncol. 2021 Dec 20;39(36):4029-4038. Epub 2021 Oct 15. link to original article link to PMC article dosing details in manuscript have been reviewed by our editors EuroPMC NCT01231906
#EE2012: Brennan B, Kirton L, Marec-Bérard P, Gaspar N, Laurence V, Martín-Broto J, Sastre A, Gelderblom H, Owens C, Fenwick N, Strauss S, Moroz V, Whelan J, Wheatley K. Comparison of two chemotherapy regimens in patients with newly diagnosed Ewing sarcoma (EE2012): an open-label, randomised, phase 3 trial. Lancet. 2022 Oct 29;400(10362):1513-1521. link to original article EuroPMC Eudra-CT 2012-002107-17
#COG AEWS1221: DuBois SG, Krailo MD, Glade-Bender J, Buxton A, Laack N, Randall RL, Chen HX, Seibel NL, Boron M, Terezakis S, Hill-Kayser C, Hayes A, Reid JM, Teot L, Rakheja D, Womer R, Arndt C, Lessnick SL, Crompton BD, Kolb EA, Daldrup-Link H, Eutsler E, Reed DR, Janeway KA, Gorlick RG. Randomized Phase III Trial of Ganitumab With Interval-Compressed Chemotherapy for Patients With Newly Diagnosed Metastatic Ewing Sarcoma: A Report From the Children's Oncology Group. J Clin Oncol. 2023 Apr 10;41(11):2098-2107. Epub 2023 Jan 20. link to original article link to PMC article EuroPMC NCT02306161
==VIDE==
VIDE: Vincristine, Ifosfamide, Doxorubicin, Etoposide 
===Regimen variant #1===
Study 	Dates of enrollment	Evidence
Juergens et al. 2006 (EURO-E.W.I.N.G. 99)	Not reported 	Phase 2
Koch et al. 2022 (Ewing 2008R3) 	2009-2018 	Non-randomized part of phase 3 RCT
====Chemotherapy====
*Vincristine (Oncovin) 1.5 mg/m2 (maximum dose of 2 mg) IV push once on day 1
*Ifosfamide (Ifex) 3000 mg/m2 IV over 1 to 3 hours once per day on days 1 to 3
*Doxorubicin (Adriamycin) 20 mg/m2 IV over 4 hours once per day on days 1 to 3
*Etoposide (Vepesid) 150 mg/m2 IV over 60 minutes once per day on days 1 to 3
====Supportive therapy====
*Mesna (Mesnex) 1000 mg/m2 IV push once on day 1; 60 minutes prior to ifosfamide, then 3000 mg/m2/day IV continuous infusion over 72 hours (total dose per cycle: 10,000 mg/m2)
*2 to 3 liters/m2 hydration per day
*Recommended, but not required: Filgrastim (Neupogen) 5 mcg/kg SC once per day on days 4 to 13, starting 24 hours after completion of chemotherapy
21-day cycle for 6 cycles
====Subsequent treatment====
*EURO-E.W.I.N.G. 99: Further therapy is dictated by patient characteristics & response; details can be found in the primary reference
*Ewing 2008R3: Surgery when feasible followed by adjuvant VAC x 8 or VAI x 8, then Treosulfan & Melphalan, then auto HSCT consolidation versus No further treatment
===Regimen variant #2===
Study 	Dates of enrollment	Evidence
Strauss et al. 2003	1998-01 to 1999-06 	Phase 2
====Chemotherapy====
*Vincristine (Oncovin) 1.4 mg/m2 (maximum dose of 2 mg) IV once on day 1
*Ifosfamide (Ifex) 3000 mg/m2 IV once per day on days 1 to 3
*Doxorubicin (Adriamycin) 20 mg/m2 IV once per day on days 1 to 3
*Etoposide (Vepesid) 150 mg/m2 IV once per day on days 1 to 3
====Supportive therapy====
*Mesna (Mesnex) 3000 mg/m2/day IV continuous infusion over 72 hours, started on day 1 (total dose per cycle: 9000 mg/m2)
21-day cycle for up to 6 cycles
====Subsequent treatment====
*Strauss et al. 2003, patients with resectable localized disease: complete surgical removal of tumors when possible, then adjuvant VAI
*Strauss et al. 2003, patients with unresectable localized disease: VAI & RT consolidation
===References===
# Strauss SJ, McTiernan A, Driver D, Hall-Craggs M, Sandison A, Cassoni AM, Kilby A, Michelagnoli M, Pringle J, Cobb J, Briggs T, Cannon S, Witt J, Whelan JS. Single center experience of a new intensive induction therapy for Ewing's family of tumors: feasibility, toxicity, and stem cell mobilization properties. J Clin Oncol. 2003 Aug 1;21(15):2974-81. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC
# EURO-E.W.I.N.G. 99: Juergens C, Weston C, Lewis I, Whelan J, Paulussen M, Oberlin O, Michon J, Zoubek A, Juergens H, Craft A. Safety assessment of intensive induction with vincristine, ifosfamide, doxorubicin, and etoposide (VIDE) in the treatment of Ewing tumors in the EURO-EWING 99 clinical trial. Pediatr Blood Cancer. 2006 Jul;47(1):22-9. link to original article dosing details in abstract have been reviewed by our editors EuroPMC
# Euro-EWING99-R1: Le Deley MC, Paulussen M, Lewis I, Brennan B, Ranft A, Whelan J, Le Teuff G, Michon J, Ladenstein R, Marec-Bérard P, van den Berg H, Hjorth L, Wheatley K, Judson I, Juergens H, Craft A, Oberlin O, Dirksen U. Cyclophosphamide compared with ifosfamide in consolidation treatment of standard-risk Ewing sarcoma: results of the randomized noninferiority Euro-EWING99-R1 trial. J Clin Oncol. 2014 Aug 10;32(23):2440-8. Epub 2014 Jun 30. link to original article does not contain dosing details EuroPMC NCT00020566
#Ewing 2008R3: Koch R, Gelderblom H, Haveman L, Brichard B, Jürgens H, Cyprova S, van den Berg H, Hassenpflug W, Raciborska A, Ek T, Baumhoer D, Egerer G, Eich HT, Renard M, Hauser P, Burdach S, Bovee J, Bonar F, Reichardt P, Kruseova J, Hardes J, Kühne T, Kessler T, Collaud S, Bernkopf M, Butterfaß-Bahloul T, Dhooge C, Bauer S, Kiss J, Paulussen M, Hong A, Ranft A, Timmermann B, Rascon J, Vieth V, Kanerva J, Faldum A, Metzler M, Hartmann W, Hjorth L, Bhadri V, Dirksen U. High-Dose Treosulfan and Melphalan as Consolidation Therapy Versus Standard Therapy for High-Risk (Metastatic) Ewing Sarcoma. J Clin Oncol. 2022 Jul 20;40(21):2307-2320. Epub 2022 Apr 15. link to original article dosing details in supplement have been reviewed by our editors EuroPMC NCT00987636
#EURO EWING 2012: Brennan B, Kirton L, Marec-Bérard P, Gaspar N, Laurence V, Martín-Broto J, Sastre A, Gelderblom H, Owens C, Fenwick N, Strauss S, Moroz V, Whelan J, Wheatley K. Comparison of two chemotherapy regimens in patients with newly diagnosed Ewing sarcoma (EE2012): an open-label, randomised, phase 3 trial. Lancet. 2022 Oct 29;400(10362):1513-1521. link to original article EuroPMC
#Ewing 2008R1: Koch R, Haveman L, Ladenstein R, Brichard B, Jürgens H, Cyprova S, van den Berg H, Hassenpflug W, Raciborska A, Ek T, Baumhoer D, Egerer G, Kager L, Renard M, Hauser P, Burdach S, Bovee JVMG, Hong AM, Reichardt P, Kruseova J, Streitbürger A, Kühne T, Kessler T, Bernkopf M, Butterfaß-Bahloul T, Dhooge C, Bauer S, Kiss J, Paulussen M, Bonar F, Ranft A, Timmermann B, Rascon J, Vieth V, Kanerva J, Faldum A, Hartmann W, Hjorth L, Bhadri VA, Metzler M, Gelderblom H, Dirksen U. Zoledronic Acid Add-on Therapy for Standard-Risk Ewing Sarcoma Patients in the Ewing 2008R1 Trial. Clin Cancer Res. 2023 Dec 15;29(24):5057-5068. link to original article EuroPMC EudraCT 2008-003658-13
=Adjuvant therapy=
==Busulfan & Melphalan, then auto HSCT==
BuMel: Busulfan & Melphalan
===Regimen===
Study 	Dates of enrollment	Evidence 	Comparator	Comparative Efficacy
Atra et al. 1997 	Not reported 	Phase 2, fewer than 20 pts	 	
Whelan et al. 2018 (R2Loc)	2000-2015 	Phase 3 (E-esc) 	VAI 	Seems to have longer OS (secondary endpoint)
====Preceding treatment====
*Neoadjuvant VIDE x 6, then surgery, then adjuvant VAI x 1
===References===
# Atra A, Whelan JS, Calvagna V, Shankar AG, Ashley S, Shepherd V, Souhami RL, Pinkerton CR. High-dose busulphan/melphalan with autologous stem cell rescue in Ewing's sarcoma. Bone Marrow Transplant. 1997 Nov;20(10):843-6. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC
# R2Loc: Whelan J, Le Deley MC, Dirksen U, Le Teuff G, Brennan B, Gaspar N, Hawkins DS, Amler S, Bauer S, Bielack S, Blay JY, Burdach S, Castex MP, Dilloo D, Eggert A, Gelderblom H, Gentet JC, Hartmann W, Hassenpflug WA, Hjorth L, Jimenez M, Klingebiel T, Kontny U, Kruseova J, Ladenstein R, Laurence V, Lervat C, Marec-Berard P, Marreaud S, Michon J, Morland B, Paulussen M, Ranft A, Reichardt P, van den Berg H, Wheatley K, Judson I, Lewis I, Craft A, Juergens H, Oberlin O; Euro-EWING-99 and EWING-2008 Investigators. High-dose chemotherapy and blood autologous stem-cell rescue compared with standard chemotherapy in localized high-risk Ewing sarcoma: results of Euro-EWING99 and Ewing-2008. J Clin Oncol. 2018 Nov 1;36(31):3110-9. Epub 2018 Sep 6. link to original article dosing details in supplement have been reviewed by our editors link to PMC article EuroPMC NCT00020566
==EVAIA==
EVAIA: Etoposide, Vincristine, Adriamycin (Doxorubicin), Ifosfamide, DActinomycin
===Regimen===
Study 	Dates of enrollment	Evidence
Paulussen et al. 2008 (EICESS-92)	1992-1997 	Non-randomized part of phase 3 RCT
Note: This regimen is intended for high-risk patients.
====Preceding treatment====
*Neoadjuvant EVAIA, then local therapy
====Chemotherapy====
*Etoposide (Vepesid) 150 mg/m2 IV once per day on days 1 to 3
*Vincristine (Oncovin) 1.5 mg/m2 IV once on day 1
*Doxorubicin (Adriamycin) 30 mg/m2 IV once per day on days 2 & 4
*Ifosfamide (Ifex) 2000 mg/m2 IV once per day on days 1 to 3
**Note: primary reference does not comment about the use of Mesna (Mesnex)
*Dactinomycin (Cosmegen) 0.5 mg/m2 IV once per day on days 1, 3, 5
21-day cycle for 10 cycles
===References===
# EICESS-92: Paulussen M, Craft AW, Lewis I, Hackshaw A, Douglas C, Dunst J, Schuck A, Winkelmann W, Köhler G, Poremba C, Zoubek A, Ladenstein R, van den Berg H, Hunold A, Cassoni A, Spooner D, Grimer R, Whelan J, McTiernan A, Jürgens H; European Intergroup Cooperative Ewing's Sarcoma Study-92. Results of the EICESS-92 Study: two randomized trials of Ewing's sarcoma treatment--cyclophosphamide compared with ifosfamide in standard-risk patients and assessment of benefit of etoposide added to standard treatment in high-risk patients. J Clin Oncol. 2008 Sep 20;26(27):4385-93. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC NCT00002516
==VACA==
VACA: Vincristine, Adriamycin (Doxorubicin), Cyclophosphamide, DActinomycin
===Regimen variant #1===
Study 	Dates of enrollment	Evidence 	Comparator	Comparative Efficacy
Paulussen et al. 2008 (EICESS-92)	1992-1997 	Phase 3 (E-switch-ic)	VAIA 	Did not meet primary endpoint of EFS36
Note: this regimen was intended for standard-risk patients.
====Preceding treatment====
*Neoadjuvant VAIA, then local therapy
====Chemotherapy====
*Vincristine (Oncovin) 1.5 mg/m2 IV once on day 1
*Doxorubicin (Adriamycin) 30 mg/m2 IV once per day on days 2 & 4
*Cyclophosphamide (Cytoxan) 1200 mg/m2 IV once on day 1
*Dactinomycin (Cosmegen) 0.5 mg/m2 IV once per day on days 1, 3, 5
21-day cycle for 10 cycles
===Regimen variant #2===
Study 	Dates of enrollment	Evidence
Paulussen et al. 2001 (CESS 86)	1986-01 to 1991-07 	Non-randomized
Note: This regimen is intended for standard risk patients.
====Preceding treatment====
*Neoadjuvant VACA, then local therapy
====Chemotherapy====
*Vincristine (Oncovin) 1.5 mg/m2 IV once per day on days 1, 8, 15, 22
*Doxorubicin (Adriamycin) 30 mg/m2 IV once per day on days 1, 2, 43, 44
*Cyclophosphamide (Cytoxan) 1200 mg/m2 IV once on day 1, then 400 mg/m2 IV once per day on days 22, 23, 24, then 1200 mg/m2 IV once on day 43
*Dactinomycin (Cosmegen) 0.5 mg/m2 IV once per day on days 22, 23, 24
====Supportive therapy====
*Mesna (Mesnex) "as appropriate"
9-week "block" for 3 blocks
===Regimen variant #3===
Study 	Dates of enrollment	Evidence
Craft et al. 1997 (ET-1)	1978-1986 	Non-randomized (RT)
====Preceding treatment====
*Induction VAC, then Cyclophosphamide, Vincristine, RT with consideration for surgery
====Chemotherapy====
*Vincristine (Oncovin) 2 mg/m2 IV once on day 1
*Doxorubicin (Adriamycin) as follows:
**Cycles 1, 3, 5, 7: 50 mg/m2 IV once on day 1
*Cyclophosphamide (Cytoxan) 600 mg/m2 (maximum dose of 1000 mg) IV once on day 1
*Dactinomycin (Cosmegen) as follows:
**Cycles 2, 4, 6, 8, 12, 14, 16, 18: 1.4 mg/m2 (maximum dose of 2 mg) IV once on day 1
21-day cycle for 18 cycles (1 year)
===References===
# ET-1: Craft AW, Cotterill SJ, Bullimore JA, Pearson D; United Kingdom Children's Cancer Study Group; Medical Research Council Bone Sarcoma Working Party. Long-term results from the first UKCCSG Ewing's Tumour Study (ET-1). Eur J Cancer. 1997 Jun;33(7):1061-9. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC
# CESS 86: Paulussen M, Ahrens S, Dunst J, Winkelmann W, Exner GU, Kotz R, Amann G, Dockhorn-Dworniczak B, Harms D, Müller-Weihrich S, Welte K, Kornhuber B, Janka-Schaub G, Göbel U, Treuner J, Voûte PA, Zoubek A, Gadner H, Jürgens H. Localized Ewing tumor of bone: final results of the cooperative Ewing's Sarcoma Study CESS 86. J Clin Oncol. 2001 Mar 15;19(6):1818-29. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC
# EICESS-92: Paulussen M, Craft AW, Lewis I, Hackshaw A, Douglas C, Dunst J, Schuck A, Winkelmann W, Köhler G, Poremba C, Zoubek A, Ladenstein R, van den Berg H, Hunold A, Cassoni A, Spooner D, Grimer R, Whelan J, McTiernan A, Jürgens H; European Intergroup Cooperative Ewing's Sarcoma Study-92. Results of the EICESS-92 Study: two randomized trials of Ewing's sarcoma treatment--cyclophosphamide compared with ifosfamide in standard-risk patients and assessment of benefit of etoposide added to standard treatment in high-risk patients. J Clin Oncol. 2008 Sep 20;26(27):4385-93. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC NCT00002516
==VAI==
VAI: Vincristine, DActinomycin, Ifosfamide
IVA: Ifosfamide, Vincristine, DActinomycin
===Regimen variant #1, capped dactinomycin===
Study 	Dates of enrollment	Evidence 	Comparator	Comparative Efficacy
Le Deley et al. 2014 (Euro-EWING99-R1)	2000-2010 	Phase 3 (C)	VAC 	Inconclusive whether non-inferior EFS
Note: to our knowledge, this regimen was not tested as an experimental arm in an RCT in this context, prior to becoming a standard comparator arm.
====Preceding treatment====
*Neoadjuvant VIDE x 6, then complete surgical excision if feasible; radiotherapy if surgery incomplete or infeasible
====Chemotherapy====
*Vincristine (Oncovin) 1.5 mg/m2 (maximum dose of 2 mg) IV once on day 1
*Dactinomycin (Cosmegen) 0.75 mg/m2 (maximum dose of 1.5 mg) IV once per day on days 1 & 2
*Ifosfamide (Ifex) 3000 mg/m2 IV once per day on days 1 & 2
====Supportive therapy====
*Mesna (Mesnex) is not described
21-day cycle for 8 cycles
===Regimen variant #2, uncapped dactinomycin===
Study 	Dates of enrollment	Evidence
Strauss et al. 2003	1998-01 to 1999-06 	Phase 2
====Preceding treatment====
*Neoadjuvant VIDE, then local therapy if it was possible
====Chemotherapy====
*Vincristine (Oncovin) 1.4 mg/m2 (maximum dose of 2 mg) IV once on day 1
*Dactinomycin (Cosmegen) 0.75 mg/m2 IV once per day on days 1 & 2
*Ifosfamide (Ifex) 3000 mg/m2 IV once per day on days 1 & 2
*If appropriate, concurrent radiation therapy given sometime during the first 3 cycles
====Supportive therapy====
*Mesna (Mesnex) 3000 mg/m2/day IV continuous infusion over 48 hours, started on day 1 (total dose per cycle: 6000 mg/m2)
21-day cycle for up to 8 cycles
===References===
# Strauss SJ, McTiernan A, Driver D, Hall-Craggs M, Sandison A, Cassoni AM, Kilby A, Michelagnoli M, Pringle J, Cobb J, Briggs T, Cannon S, Witt J, Whelan JS. Single center experience of a new intensive induction therapy for Ewing's family of tumors: feasibility, toxicity, and stem cell mobilization properties. J Clin Oncol. 2003 Aug 1;21(15):2974-81. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC
# Euro-EWING99-R1: Le Deley MC, Paulussen M, Lewis I, Brennan B, Ranft A, Whelan J, Le Teuff G, Michon J, Ladenstein R, Marec-Bérard P, van den Berg H, Hjorth L, Wheatley K, Judson I, Juergens H, Craft A, Oberlin O, Dirksen U. Cyclophosphamide compared with ifosfamide in consolidation treatment of standard-risk Ewing sarcoma: results of the randomized noninferiority Euro-EWING99-R1 trial. J Clin Oncol. 2014 Aug 10;32(23):2440-8. Epub 2014 Jun 30. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC NCT00020566
==VAIA==
VAIA: Vincristine, Adriamycin (Doxorubicin), Ifosfamide, Actinomycin-D (Dactinomycin)
===Regimen variant #1===
Study 	Dates of enrollment	Evidence 	Comparator 	Comparative Efficacy
Paulussen et al. 2008 (EICESS-92)	1992-1997 	Phase 3 (C)	VACA (standard-risk)	Did not meet primary endpoint of EFS36
Note: standard-risk patients were randomized to this regimen versus VACA. High-risk patients were not randomized at this point of the protocol. 
====Preceding treatment====
*Neoadjuvant VAIA, then local therapy
====Chemotherapy====
*Vincristine (Oncovin) 1.5 mg/m2 IV once on day 1
*Doxorubicin (Adriamycin) 30 mg/m2 IV once per day on days 2 & 4
*Ifosfamide (Ifex) 2000 mg/m2 IV once per day on days 1 to 3
**Note: primary reference does not comment about the use of mesna
*Dactinomycin (Cosmegen) 0.5 mg/m2 IV once per day on days 1, 3, 5
21-day cycle for 10 cycles
===Regimen variant #2===
Study 	Dates of enrollment	Evidence
Paulussen et al. 2001 (CESS 86)	1986-01 to 1991-07 	Non-randomized
Note: This regimen was intended for high risk patients.
====Preceding treatment====
*Neoadjuvant VAIA, then local therapy
====Chemotherapy====
*Vincristine (Oncovin) 1.5 mg/m2 IV once per day on days 1, 8, 15, 22
*Doxorubicin (Adriamycin) 30 mg/m2 IV once per day on days 1, 2, 43, 44
*Ifosfamide (Ifex) 3000 mg/m2 IV once per day on days 1, 2, 22, 23, 43, 44
*Dactinomycin (Cosmegen) 0.5 mg/m2 IV once per day on days 22, 23, 24
====Supportive therapy====
*Mesna (Mesnex) "as appropriate"
9-week "block" for 3 blocks
===References===
# CESS 86: Paulussen M, Ahrens S, Dunst J, Winkelmann W, Exner GU, Kotz R, Amann G, Dockhorn-Dworniczak B, Harms D, Müller-Weihrich S, Welte K, Kornhuber B, Janka-Schaub G, Göbel U, Treuner J, Voûte PA, Zoubek A, Gadner H, Jürgens H. Localized Ewing tumor of bone: final results of the cooperative Ewing's Sarcoma Study CESS 86. J Clin Oncol. 2001 Mar 15;19(6):1818-29. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC
# EICESS-92: Paulussen M, Craft AW, Lewis I, Hackshaw A, Douglas C, Dunst J, Schuck A, Winkelmann W, Köhler G, Poremba C, Zoubek A, Ladenstein R, van den Berg H, Hunold A, Cassoni A, Spooner D, Grimer R, Whelan J, McTiernan A, Jürgens H; European Intergroup Cooperative Ewing's Sarcoma Study-92. Results of the EICESS-92 Study: two randomized trials of Ewing's sarcoma treatment--cyclophosphamide compared with ifosfamide in standard-risk patients and assessment of benefit of etoposide added to standard treatment in high-risk patients. J Clin Oncol. 2008 Sep 20;26(27):4385-93. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC NCT00002516
=Relapsed or refractory or metastatic=
==Busulfan & Melphalan, then auto HSCT==
===Regimen variant #1, PO busulfan, mel 140 mg/m2===
Study 	Dates of enrollment	Evidence
Atra et al. 1997	Not reported 	Phase 2, fewer than 20 pts
===Regimen variant #2, PO busulfan, mel 160 mg/m2===
Study 	Dates of enrollment	Evidence
Atra et al. 1997	Not reported 	Phase 2, fewer than 20 pts
===Regimen variant #3, IV busulfan===
Study 	Dates of enrollment	Evidence
Strauss et al. 2003	1998-01 to 1999-06 	Phase 2
Note that melphalan is reported as given on day 2 (not day -2) in the original reference but this is surely an error.
====Preceding treatment====
*Adjuvant VAI x 1 or more cycles
===References===
# Atra A, Whelan JS, Calvagna V, Shankar AG, Ashley S, Shepherd V, Souhami RL, Pinkerton CR. High-dose busulphan/melphalan with autologous stem cell rescue in Ewing's sarcoma. Bone Marrow Transplant. 1997 Nov;20(10):843-6. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC
# Strauss SJ, McTiernan A, Driver D, Hall-Craggs M, Sandison A, Cassoni AM, Kilby A, Michelagnoli M, Pringle J, Cobb J, Briggs T, Cannon S, Witt J, Whelan JS. Single center experience of a new intensive induction therapy for Ewing's family of tumors: feasibility, toxicity, and stem cell mobilization properties. J Clin Oncol. 2003 Aug 1;21(15):2974-81. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC
==Cyclophosphamide & Topotecan==
===Regimen variant #1, standard-dose===
Study 	Dates of enrollment	Evidence
Saylors et al. 2001	Not reported 	Phase 2
Note: Some guidelines state that Vincristine (Oncovin) can be added to this regimen. No primary reference for this is available.
====Chemotherapy====
*Cyclophosphamide (Cytoxan) 250 mg/m2 IV over 30 minutes once per day on days 1 to 5, given first
*Topotecan (Hycamtin) 0.75 mg/m2 IV over 30 minutes once per day on days 1 to 5, given second
====Supportive therapy====
*500 mL/m/2 fluids IV or PO once per day on days 1 to 5; 2 to 4 hours prior to chemotherapy
*Antiemetics once per day on days 1 to 5, prior to chemotherapy
*3 liters/m2 IV or PO over 24 hours after chemotherapy
*Filgrastim (Neupogen) 5 mcg/kg SC once per day, starting on day 6, to continue until ANC is at least 1500/μL above nadir
21-day cycle for 12 to 14 cycles
===Regimen variant #2, standard-dose with local therapy===
Study 	Dates of enrollment	Evidence
Hunold et al. 2006	1998-09 to 2004-04 	Phase 2
Note: Some guidelines state that vincristine can be added to this regimen. No primary reference for this is available.
====Chemotherapy====
*Cyclophosphamide (Cytoxan) 250 mg/m2 IV over 30 minutes once per day on days 1 to 5
*Topotecan (Hycamtin) 0.75 mg/m2 IV over 30 minutes once per day on days 1 to 5
====Supportive therapy====
*Mesna (Mesnex), antiemetics, fluids, and Filgrastim (Neupogen) "according to institutional standards"
21-day cycle for 12 to 14 cycles
====Subsequent treatment====
*Hunold et al. 2006, surgical candidate lesions: Surgical removal of tumors is done when possible.
*Hunold et al. 2006, all other lesions: Definitive External beam radiotherapy
===Regimen variant #3, high-dose===
Study 	Dates of enrollment	Evidence
Kushner et al. 2000	Not reported 	Non-randomized
Note: Some guidelines state that Vincristine (Oncovin) can be added to this regimen. No primary reference for this is available.
====Chemotherapy====
*Cyclophosphamide (Cytoxan) 2100 mg/m2/day IV continuous infusion over 48 hours, started on day 1, given second (total dose per cycle: 4200 mg/m2)
**Children 10 years or younger received 70 mg/kg/day IV continuous infusion over 48 hours, started on day 1 (total dose per cycle: 140 mg/kg)
*Topotecan (Hycamtin) 2 mg/m2/day IV continuous infusion over 72 hours, started on day 1, given third (total dose per cycle: 6 mg/m2)
====Supportive therapy====
*Mesna (Mesnex) 2100 mg/m2/day IV continuous infusion over 72 hours, started on day 1, given first (total dose per cycle: 6300 mg/m2)
**Children 10 years or younger received 70 mg/kg/day IV continuous infusion over 72 hours, started on day 1 (total dose per cycle: 210 mg/kg)
***If body surface area less than 1 m2, mesna is given in 500 mL NS over 24 hours
***If body surface area is at least 1 m2, mesna is given in 1000 mL NS over 24 hours
*On day 1, prior to chemotherapy, 20 mL/kg NS IV over 30 minutes, then D5 1/2 NS with 15 mEq KCl per 500 mL at 200 mL/m2/H until urine specific gravity less than 1.010, then start mesna & cyclophosphamide
*Additional hydration fluid on days 1 & 2 so that, when added to volumes of cyclophosphamide, mesna, and topotecan, total volume of fluids is 3000 mL/m2/24 hours
*Additional hydration fluid on day 3 at 150 mL/m2/hour for 6 to 12 hours after completion of cyclophosphamide infusion
*Cyclophosphamide is given in D5NS with 10 mEq potassium chloride (KCl) and 5 mg Furosemide (Lasix) per 500 mL fluid. 500 mL total volume is used for patients with body surface area less than 1 m2; 1000 mL total volume is used for patients with BSA of at least 1 m2
*Filgrastim (Neupogen) 5 mcg/kg SC once per day, starting on day 5, to continue until ANC is at least 1000/μL
Subsequent cycles to start when ANC greater than 1000/μL and platelets greater than 75 x 109/L
===References===
# Kushner BH, Kramer K, Meyers PA, Wollner N, Cheung NK. Pilot study of topotecan and high-dose cyclophosphamide for resistant pediatric solid tumors. Med Pediatr Oncol. 2000 Nov;35(5):468-74. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC
# Saylors RL 3rd, Stine KC, Sullivan J, Kepner JL, Wall DA, Bernstein ML, Harris MB, Hayashi R, Vietti TJ; Pediatric Oncology Group. Cyclophosphamide plus topotecan in children with recurrent or refractory solid tumors: a Pediatric Oncology Group phase II study. J Clin Oncol. 2001 Aug 1;19(15):3463-9. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC
# Hunold A, Weddeling N, Paulussen M, Ranft A, Liebscher C, Jürgens H. Topotecan and cyclophosphamide in patients with refractory or relapsed Ewing tumors. Pediatr Blood Cancer. 2006 Nov;47(6):795-800. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC
</div>
==Docetaxel & Gemcitabine==
===Regimen===
Study 	Dates of enrollment	Evidence
Navid et al. 2008	Not reported 	Retrospective
Note: Some guidelines state that Vincristine (Oncovin) can be added to this regimen. No primary reference for this is available. Only 2 of the 22 patients in this retrospective review had Ewing sarcoma.
====Chemotherapy====
*Docetaxel (Taxotere) 75 to 100 mg/m2 IV over 60 minutes once on day 8, given second
*Gemcitabine (Gemzar) 675 mg/m2 IV over 90 minutes once per day on days 1 & 8, given first on day 8
====Supportive therapy====
*Ondansetron (Zofran) once per day on days 1 & 8, prior to chemotherapy
*Dexamethasone (Decadron) starting either the day before or the day of docetaxel, and continued for 2 days after docetaxel
*H1 or H2 blockers such as Diphenhydramine (Benadryl) and Ranitidine (Zantac) prior to chemotherapy on days 1 & 8 per physician discretion
*Some patients received Filgrastim (Neupogen) starting on day 9
21-day cycles
===References===
# Retrospective: Navid F, Willert JR, McCarville MB, Furman W, Watkins A, Roberts W, Daw NC. Combination of gemcitabine and docetaxel in the treatment of children and young adults with refractory bone sarcoma. Cancer. 2008 Jul 15;113(2):419-25. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC
==ICE==
ICE: Ifosfamide, Carboplatin, Etoposide
===Regimen===
Study 	Dates of enrollment	Evidence
Van Winkle et al. 2005 (CCG-0894)	1992-06 to 1994-11 	Phase 2
Van Winkle et al. 2005 (CCG-0924)	1993-07 to 1995-04 	Phase 1, >20 pts
Van Winkle et al. 2005 (CCG-0931)	1994-05 to 1996-12 	Non-randomized, <20 pts
Note: Some guidelines state that Vincristine (Oncovin) can be added to this regimen. No primary reference for this is available. The reference did not mention Mesna (Mesnex) being used.
====Chemotherapy====
*Ifosfamide (Ifex) 1800 mg/m2 IV once per day on days 1 to 5
*Carboplatin (Paraplatin) 400 mg/m2 IV "for 2 days"
**Note: the reference did not explicitly say which 2 days carboplatin should be given on
*Etoposide (Vepesid) 100 mg/m2 IV once per day on days 1 to 5
====Supportive therapy====
*Depending on the study the patients were enrolled on, they received one of the following:
**CCG-0894: Filgrastim (Neupogen) 5 or 10 mcg/kg SC once per day, starting 24 hours after completing ICE, and to continue until day 18 if ANC is at least 1000/μL, or until ANC is at least 1000/μL above nadir, whichever comes later
**CCG-0924: PIXY 321 at doses of 500/750/1000 mcg/m2 SC once per day or 500 mcg/m2 SC twice per day, starting on day 5 and to continue until day 18 unless ANC reached 20,000/μL or platelet count is at least 900 x 109/L for 2 days between days 13 to 18, or until ANC is at least 1000/μL and platelet count is at least 100 x 109/L, whichever comes later
**CCG-0931: Filgrastim (Neupogen) 5 mcg/kg SC once per day and IL-6 at 2.5, 3.75, or 5 mcg/kg SC twice per day, starting 24 hours after completing ICE. Filgrastim is continued until ANC is at least 1000/μL, and IL-6 is continued until platelets are at least 100 x 109/L for 2 consecutive days or until day 35, whichever comes sooner.
21-day cycles, with next cycle starting as soon as ANC is at least 1000/μL and platelet count is at least 100 x 109/L
====Subsequent treatment====
*Resection of disease was allowed after 4 cycles based on patient's response to ICE
===References===
# CCG-0894: Van Winkle P, Angiolillo A, Krailo M, Cheung YK, Anderson B, Davenport V, Reaman G, Cairo MS; Children's Cancer Group. Ifosfamide, carboplatin, and etoposide (ICE) reinduction chemotherapy in a large cohort of children and adolescents with recurrent/refractory sarcoma: the Children's Cancer Group (CCG) experience. Pediatr Blood Cancer. 2005 Apr;44(4):338-47. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC
# CCG-0924: Van Winkle P, Angiolillo A, Krailo M, Cheung YK, Anderson B, Davenport V, Reaman G, Cairo MS; Children's Cancer Group. Ifosfamide, carboplatin, and etoposide (ICE) reinduction chemotherapy in a large cohort of children and adolescents with recurrent/refractory sarcoma: the Children's Cancer Group (CCG) experience. Pediatr Blood Cancer. 2005 Apr;44(4):338-47. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC
# CCG-0931: Van Winkle P, Angiolillo A, Krailo M, Cheung YK, Anderson B, Davenport V, Reaman G, Cairo MS; Children's Cancer Group. Ifosfamide, carboplatin, and etoposide (ICE) reinduction chemotherapy in a large cohort of children and adolescents with recurrent/refractory sarcoma: the Children's Cancer Group (CCG) experience. Pediatr Blood Cancer. 2005 Apr;44(4):338-47. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC
==IE==
IE: Ifosfamide, Etoposide
===Regimen===
Study 	Dates of enrollment	Evidence
Miser et al. 1987	1985-06 to 1986-06 	Phase 2
Note: Some guidelines state that Vincristine (Oncovin) can be added to this regimen. No primary reference for this is available.
====Chemotherapy====
*Ifosfamide (Ifex) 1800 mg/m2 IV once per day on days 1 to 5, given second, with loading dose of mesna
*Etoposide (Vepesid) 100 mg/m2 IV over 60 minutes once per day on days 1 to 5, given first
====Supportive therapy====
*Mesna (Mesnex) 360 mg/m2 IV loading dose over 1 hour, given with ifosfamide, then 120 mg/m2/hour IV over 3 hours, then 360 mg/m2 IV or PO over 15 minutes every 3 hours for 6 doses, given at hours 5, 8, 11, 14, 17, 20
21-day cycle for 12 cycles
====Subsequent treatment====
*Miser et al. 1987, patients responding to therapy after 4 cycles: local therapy with surgery or radiation is used to try to achieve a complete remission. 
**Radiation therapy consisted of 180 cGy fractions given for a total dose of 50 to 5500 cGy.
===References===
# Miser JS, Kinsella TJ, Triche TJ, Tsokos M, Jarosinski P, Forquer R, Wesley R, Magrath I. Ifosfamide with mesna uroprotection and etoposide: an effective regimen in the treatment of recurrent sarcomas and other tumors of children and young adults. J Clin Oncol. 1987 Aug;5(8):1191-8. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC
==Irinotecan & Temozolomide==
===Regimen variant #1===
Study 	Dates of enrollment	Evidence
Wagner et al. 2004	2002-01 to 2003-02 	Phase 1, fewer than 20 pts
Note: Some guidelines state that Vincristine (Oncovin) can be added to this regimen. No primary reference for this is available. Note that irinotecan 15 mg/m2 was also studied, but this dose was not recommended due to dose-limiting toxicities of diarrhea and infection.
====Chemotherapy====
*Irinotecan (Camptosar) 10 mg/m2 IV over 60 minutes once per day on days 1 to 5, 8 to 12, given second on days 1 to 5, 1 hour after temozolomide
*Temozolomide (Temodar) 100 mg/m2 PO once per day on days 1 to 5, given first
====Supportive therapy====
*Loperamide (Imodium) prn diarrhea
28-day cycles
===Regimen variant #2===
Study 	Dates of enrollment	Evidence
Casey et al. 2009	2006-01 to 2009-04 	Retrospective
Note: Some guidelines state that Vincristine (Oncovin) can be added to this regimen. No primary reference for this is available.
====Chemotherapy====
*Irinotecan (Camptosar) 20 mg/m2 IV over 60 minutes once per day on days 1 to 5, 8 to 12, given second on days 1 to 5, 1 hour after temozolomide
*Temozolomide (Temodar) 100 mg/m2 PO once per day on days 1 to 5, given first
====Supportive therapy====
*Cefixime (Suprax) prophylaxis starting 1 to 2 days prior to irinotecan, continuing until the completion of each cycle
*Activated charcoal, with 5x the dose in mg of the irinotecan dose, maximum of 260 mg PO three times per day during irinotecan therapy
*Loperamide (Imodium) prn diarrhea
*Patient "advised to maintain hydration"
21-day cycles
===References===
# Phase I: Wagner LM, Crews KR, Iacono LC, Houghton PJ, Fuller CE, McCarville MB, Goldsby RE, Albritton K, Stewart CF, Santana VM. Phase I trial of temozolomide and protracted irinotecan in pediatric patients with refractory solid tumors. Clin Cancer Res. 2004 Feb 1;10(3):840-8. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC
# Retrospective: Wagner LM, McAllister N, Goldsby RE, Rausen AR, McNall-Knapp RY, McCarville MB, Albritton K. Temozolomide and intravenous irinotecan for treatment of advanced Ewing sarcoma. Pediatr Blood Cancer. 2007 Feb;48(2):132-9. link to original article EuroPMC
# Retrospective: Casey DA, Wexler LH, Merchant MS, Chou AJ, Merola PR, Price AP, Meyers PA. Irinotecan and temozolomide for Ewing sarcoma: the Memorial Sloan-Kettering experience. Pediatr Blood Cancer. 2009 Dec;53(6):1029-34. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC
==TC, then IE, VDoxoC, VEC==
TC, then IE, VDoxoC, VEC: Topotecan, Cyclophosphamide followed by Ifosfamide, Etoposide, then Vincristine, Doxorubicin, Cyclophosphamide, then Vincristine, Etoposide, Cyclophosphamide
Study 	Dates of enrollment	Evidence
Bernstein et al. 2006 (POG 9457)	Not reported 	Phase 2
Note: This was a complex regimen, and it is suggested to refer to the primary reference and figure 1 for the protocol schema. One arm of patients in this trial received Amifostine (Ethyol), but its usage is not described below since it did not result in improved outcomes. Treatment starts with an optional topotecan window for stable patients without significantly impaired function or life-threatening disease:
===Topotecan window===
====Chemotherapy====
*Topotecan (Hycamtin) 2.4 mg/m2 IV once per day on days 1 to 5
====Supportive therapy====
*Filgrastim (Neupogen) 5 mcg/kg SC once per day, starting on day 6, to continue until ANC is at least 5000/μL above nadir
5-day course, followed by upfront window, starting at week 0:
===Upfront window===
====Chemotherapy====
*Topotecan (Hycamtin) 0.75 mg/m2 IV over 30 minutes once per day on days 1 to 5
*Cyclophosphamide (Cytoxan) 250 mg/m2 IV over 30 minutes once per day on days 1 to 5, given first
====Supportive therapy====
*Prehydration with 500 mL/m2 D5 1/4 NS
*1500 mL/m2 IV or PO hydration continuous for 24 hours after chemotherapy
*Filgrastim (Neupogen) 5 mcg/kg SC once per day, starting on day 6, to continue until ANC is at least 5000/μL above nadir
21-day cycle for up to 2 cycles
Patients with progression after the first cycle moved immediately to induction therapy; others proceeded to induction after the second cycle, starting at week 6 with IE:
===Induction===
====Chemotherapy, IE portion====
*Ifosfamide (Ifex) as follows:
**Cycle 1: 3600 mg/m2 IV over 2 hours once per day on days 1 to 5, given second, after etoposide
***Administered in 200 mL/m2 D5 1/2 NS
**Cycles 2 & 3: 2800 mg/m2 IV over 2 hours once per day on days 1 to 5, given second, after etoposide
***Administered in 200 mL/m2 D5 1/2 NS
*Etoposide (Vepesid) 100 mg/m2 IV over 45 minutes once per day on days 1 to 5, given first, before ifosfamide
**Administered in 250 mL/m2 of D5 1/2 NS
====Supportive therapy, IE portion====
*Mesna (Mesnex) 4000 mg/m2 IV once per day on days 1 to 5
*"Vigorous hydration" 
*Antiemetics
*Filgrastim (Neupogen) 5 mcg/kg SC once per day, starting 24 to 48 hours after completion of chemotherapy
21-day cycle for a total of 3 cycles, alternating with VDoxoC
====Chemotherapy, VDoxoC portion====
*Vincristine (Oncovin) 2 mg/m2 (maximum dose of 2 mg) IV bolus once per day on days 1, 8, 15, given first
*Doxorubicin (Adriamycin) 37.5 mg/m2/day IV continuous infusion over 48 hours, started on day 1, given third (total dose per cycle: 75 mg/m2)
**Administered in 2400 mL/m2/day (4800 mL/m2 total volume) of D5 1/2 NS
*Cyclophosphamide (Cytoxan) 2100 mg/m2 IV over 30 minutes once per day on days 1 & 2, given second
**Administered in 200 mL/m2 D5 1/2 NS
====Supportive therapy, VDoxoC portion====
*Mesna (Mesnex) 2400 mg/m2 total dose IV; exact schedule not specified by reference
*Filgrastim (Neupogen) 5 mcg/kg SC once per day, starting on day 4, 24 hours after chemotherapy is complete
21-day cycle for a total of 2 cycles, alternating with IE
Local therapy for primary disease along with ongoing chemotherapy starts at week 21:
===Definitive therapy, primary===
====Chemotherapy, VDoxoC portion====
*Vincristine (Oncovin) 2 mg/m2 (maximum dose of 2 mg) IV once per day on days 1 & 8
*Doxorubicin (Adriamycin) 37.5 mg/m2/day IV continuous infusion over 48 hours, started on day 1 (total dose per cycle: 75 mg/m2)
*Cyclophosphamide (Cytoxan) 1500 mg/m2 IV once on day 1
====Supportive therapy, VDoxoC portion====
*Mesna (Mesnex), dosage & schedule not specified by reference
*Filgrastim (Neupogen) 5 mcg/kg SC once per day, starting 24 to 48 hours after completion of chemotherapy
21-day course, followed by local control:
====Local therapy, after week 21====
*Choice of modality between surgical and radiation therapy options is at the discretion of the provider
*POG 9457, patients treated with radiation alone: External beam radiotherapy 4500 cGy in 180 cGy fractions to the initial tumor volume; additional treatment up to a total of 5580 cGy was administered to original bony tumors and the postinduction chemotherapy soft tissue volumes plus a 2 cm margin
*See primary reference for details about radiation therapy in a variety of clinical scenarios
====Chemotherapy, VEC portion====
*Vincristine (Oncovin) 2 mg/m2 (maximum dose of 2 mg) IV once per day on days 1 & 8
*Etoposide (Vepesid) 150 mg/m2 IV once per day on days 1 to 3
*Cyclophosphamide (Cytoxan) 1500 mg/m2 IV once on day 1
====Supportive therapy, VEC portion====
*Use of Mesna (Mesnex) not specified by reference
*Filgrastim (Neupogen) 5 mcg/kg SC once per day, starting 24 to 48 hours after completion of chemotherapy
21-day cycle for 2 cycles, followed by:
===Continuation===
====Chemotherapy, IE portion====
*Ifosfamide (Ifex) 2100 mg/m2 IV once per day on days 1 to 5
*Etoposide (Vepesid) 100 mg/m2 IV once per day on days 1 to 5
====Supportive therapy, IE portion====
*Mesna (Mesnex), dosage & schedule not specified by reference
*Filgrastim (Neupogen) 5 mcg/kg SC once per day, starting 24 to 48 hours after completion of chemotherapy
21-day cycle for a total of 2 cycles, alternating with VDoxoC
====Chemotherapy, VDoxoC portion====
*Vincristine (Oncovin) 2 mg/m2 (maximum dose of 2 mg) IV once per day on days 1, 8, 15
*Doxorubicin (Adriamycin) 37.5 mg/m2/day IV continuous infusion over 48 hours, started on day 1 (total dose per cycle: 75 mg/m2)
*Cyclophosphamide (Cytoxan) 1500 mg/m2 IV once on day 1
====Supportive therapy, VDoxoC portion====
*Mesna (Mesnex) dosage & schedule not specified by reference
*Filgrastim (Neupogen) 5 mcg/kg SC once per day, starting 24 to 48 hours after completion of chemotherapy
21-day course, in between IE
Local therapy for metastatic disease along with ongoing chemotherapy starts at week 39:
===Definitive therapy, metastases===
====Chemotherapy, VDoxoC potion====
*Vincristine (Oncovin) 2 mg/m2 (maximum dose of 2 mg) IV once per day on days 1 & 8
**Note: the day 8 dose is not described in the text but is described in figure 1 
*Doxorubicin (Adriamycin) 37.5 mg/m2/day IV continuous infusion over 48 hours, started on day 1 (total dose per cycle: 75 mg/m2)
*Cyclophosphamide (Cytoxan) 1500 mg/m2 IV once on day 1
====Supportive therapy, VDoxoC portion====
*Mesna (Mesnex) dosage & schedule not specified by reference
*Filgrastim (Neupogen) 5 mcg/kg SC once per day, starting 24 to 48 hours after completion of chemotherapy
21-day course, followed by local control of metastatic disease:
====Local therapy, metastatic disease (after week 39)====
*Choice of modality between surgical and radiation therapy options is at the discretion of the provider
*External beam radiotherapy could be used to treat up to three sites of metastatic disease
*See primary reference for details about radiation therapy in a variety of clinical scenarios
====Chemotherapy, VEC portion====
*Vincristine (Oncovin) 2 mg/m2 (maximum dose of 2 mg) IV once per day on days 1 & 8
*Etoposide (Vepesid) 150 mg/m2 IV once per day on days 1 to 3
*Cyclophosphamide (Cytoxan) 1500 mg/m2 IV once on day 1
====Supportive therapy, VEC portion====
*Use of Mesna (Mesnex) not specified by reference
*Filgrastim (Neupogen) 5 mcg/kg SC once per day, starting 24 to 48 hours after completion of chemotherapy
21-day cycle for 2 cycles
===References===
# POG 9457: Bernstein ML, Devidas M, Lafreniere D, Souid AK, Meyers PA, Gebhardt M, Stine K, Nicholas R, Perlman EJ, Dubowy R, Wainer IW, Dickman PS, Link MP, Goorin A, Grier HE; Pediatric Oncology Group; Children's Cancer Group; Children's Oncology Group. Intensive therapy with growth factor support for patients with Ewing tumor metastatic at diagnosis: Pediatric Oncology Group/Children's Cancer Group Phase II Study 9457--a report from the Children's Oncology Group. J Clin Oncol. 2006 Jan 1;24(1):152-9. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC NCT00002643
==VAdCA==
VAdCA: Vincristine, Adriamycin (Doxorubicin), Cyclophosphamide, DActinomycin
===Regimen===
Study 	Dates of enrollment	Evidence 	Comparator	Comparative Efficacy
Miser et al. 2004	1988-1992 	Phase 3 (C)	VAdCA/IE 	Did not meet co-primary endpoints of EFS/OS
Note: this is essentially a sub-group analysis of the protocol published in Grier et al. 2003, but some key details differ, so we report it separately.
====Chemotherapy====
*Vincristine (Oncovin) 2 mg/m2 IV once on day 1
**Note: Miser et al. 2004 does not say the dose is capped at a maximum dose of 2 mg, but Grier et al. 2003 uses a capped dose and is from the same trial
*Doxorubicin (Adriamycin) 75 mg/m2 IV once on day 1
**Stop once cumulative dose received by the patient exceeds 375 mg/m2 (after 5 courses)
*Cyclophosphamide (Cytoxan) 1200 mg/m2 IV once on day 1
*Dactinomycin (Cosmegen) 1.25 mg/m2 IV once on day 1, once cumulative doxorubicin dose received by the patient exceeds 375 mg/m2
21-day cycle for 17 cycles
====Local therapy====
Local therapy is planned to take place on week 9, as follows:
*Surgical removal of tumors is done when possible.
*External beam radiotherapy to all metastatic sites of disease in addition to any radiation planned for primary tumor.
*If only radiation therapy is used, External beam radiotherapy 4500 cGy is administered to the tumor volume plus a 3 cm margin, followed by 1080 cGy to only the preradiation tumor volume, for a total dose of 5580 cGy
*Residual tumor after surgery and lung metastases are treated with "dose-volume guidelines for gross residual disease"
===References===
# Miser JS, Krailo MD, Tarbell NJ, Link MP, Fryer CJ, Pritchard DJ, Gebhardt MC, Dickman PS, Perlman EJ, Meyers PA, Donaldson SS, Moore S, Rausen AR, Vietti TJ, Grier HE; Children's Cancer Group; Pediatric Oncology Group. Treatment of metastatic Ewing's sarcoma or primitive neuroectodermal tumor of bone: evaluation of combination ifosfamide and etoposide--a Children's Cancer Group and Pediatric Oncology Group study. J Clin Oncol. 2004 Jul 15;22(14):2873-6. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC
==VAdCA/IE==
VAdCA/IE: Vincristine, Adriamycin (Doxorubicin), Cyclophosphamide, DActinomycin alternating with Ifosfamide, Etoposide
===Regimen===
Study 	Dates of enrollment	Evidence 	Comparator	Comparative Efficacy
Miser et al. 2004	1988-1992 	Phase 3 (E-esc)	VAdCA 	Did not meet co-primary endpoints of EFS/OS
Note: this is essentially a sub-group analysis of the protocol published in Grier et al. 2003, but some key details differ, so we report it separately.
====Chemotherapy, VAdCA portion (cycles 1, 3, 5, 7, 9, 11, 13, 15, 17)====
*Vincristine (Oncovin) 2 mg/m2 IV once on day 1
**Note: Miser et al. 2004 does not say the dose is capped at a maximum dose of 2 mg, but Grier et al. 2003 uses a capped dose and is from the same trial
*Doxorubicin (Adriamycin) 75 mg/m2 IV once on day 1
**Stop once cumulative dose received by the patient exceeds 375 mg/m2 (after 5 courses)
*Cyclophosphamide (Cytoxan) 1200 mg/m2 IV once on day 1
*Dactinomycin (Cosmegen) 1.25 mg/m2 IV once on day 1, once cumulative doxorubicin dose received by the patient exceeds 375 mg/m2
====Chemotherapy, IE portion (cycles 2, 4, 6, 8, 10, 12, 14, 16)====
*Ifosfamide (Ifex) 1800 mg/m2 IV once per day on days 1 to 5, with mesna
*Etoposide (Vepesid) 100 mg/m2 IV once per day on days 1 to 5
====Supportive therapy, IE portion (cycles 2, 4, 6, 8, 10, 12, 14, 16)====
*Mesna (Mesnex) with ifosfamide; primary reference did not list dosage/schedule
21-day cycle for 17 cycles
====Local therapy====
Local therapy is planned to take place on week 9, as follows:
*Surgical removal of tumors is done when possible.
*External beam radiotherapy to all metastatic sites of disease in addition to any radiation planned for primary tumor.
*If only radiation therapy is used, External beam radiotherapy 4500 cGy is administered to the tumor volume plus a 3 cm margin, followed by 1080 cGy to only the preradiation tumor volume, for a total dose of 5580 cGy
*Residual tumor after surgery and lung metastases are treated with "dose-volume guidelines for gross residual disease"
===References===
# Miser JS, Krailo MD, Tarbell NJ, Link MP, Fryer CJ, Pritchard DJ, Gebhardt MC, Dickman PS, Perlman EJ, Meyers PA, Donaldson SS, Moore S, Rausen AR, Vietti TJ, Grier HE; Children's Cancer Group; Pediatric Oncology Group. Treatment of metastatic Ewing's sarcoma or primitive neuroectodermal tumor of bone: evaluation of combination ifosfamide and etoposide--a Children's Cancer Group and Pediatric Oncology Group study. J Clin Oncol. 2004 Jul 15;22(14):2873-6. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC
==VAI==
VAI: Vincristine, DActinomycin, Ifosfamide
===Regimen===
Study 	Dates of enrollment	Evidence
Strauss et al. 2003	1998-01 to 1999-06 	Phase 2
Note: This protocol was intended for patients with metastatic disease. The reference does not clearly describe how many cycles of VAI were used.
====Preceding treatment====
*Induction VIDE for up to 6 cycles
====Chemotherapy====
*Vincristine (Oncovin) 1.4 mg/m2 (maximum dose of 2 mg) IV once on day 1
*Dactinomycin (Cosmegen) 0.75 mg/m2 IV once per day on days 1 & 2
*Ifosfamide (Ifex) 3000 mg/m2 IV once per day on days 1 & 2
====Supportive therapy====
*Mesna (Mesnex) 3000 mg/m2/day IV continuous infusion over 48 hours, started on day 1 (total dose per cycle: 6000 mg/m2)
21-day cycle for one or more cycles
====Subsequent treatment====
*Busulfan & Melphalan, then auto HSCT consolidation
===References===
# Strauss SJ, McTiernan A, Driver D, Hall-Craggs M, Sandison A, Cassoni AM, Kilby A, Michelagnoli M, Pringle J, Cobb J, Briggs T, Cannon S, Witt J, Whelan JS. Single center experience of a new intensive induction therapy for Ewing's family of tumors: feasibility, toxicity, and stem cell mobilization properties. J Clin Oncol. 2003 Aug 1;21(15):2974-81. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC
# R2Pulm: Dirksen U, Brennan B, Le Deley MC, Cozic N, van den Berg H, Bhadri V, Brichard B, Claude L, Craft A, Amler S, Gaspar N, Gelderblom H, Goldsby R, Gorlick R, Grier HE, Guinbretiere JM, Hauser P, Hjorth L, Janeway K, Juergens H, Judson I, Krailo M, Kruseova J, Kuehne T, Ladenstein R, Lervat C, Lessnick SL, Lewis I, Linassier C, Marec-Berard P, Marina N, Morland B, Pacquement H, Paulussen M, Randall RL, Ranft A, Le Teuff G, Wheatley K, Whelan J, Womer R, Oberlin O, Hawkins DS; Euro-E.W.I.N.G. 99 and Ewing 2008 Investigators. High-Dose Chemotherapy Compared With Standard Chemotherapy and Lung Radiation in Ewing Sarcoma With Pulmonary Metastases: Results of the European Ewing Tumour Working Initiative of National Groups, 99 Trial and EWING 2008. J Clin Oncol. 2019 Dec 1;37(34):3192-3202. Epub 2019 Sep 25. link to original article link to PMC article EuroPMC NCT00987636
==VIDE==
VIDE: Vincristine, Ifosfamide, Doxorubicin, Etoposide
===Regimen===
Study 	Dates of enrollment	Evidence	Efficacy
Strauss et al. 2003	1998-01 to 1999-06 	Phase 2 	ORR: 88%
Note: This protocol was intended for patients with metastatic disease.
====Chemotherapy====
*Vincristine (Oncovin) 1.4 mg/m2 (maximum dose of 2 mg) IV once on day 1
*Ifosfamide (Ifex) 3000 mg/m2 IV once per day on days 1 to 3
*Doxorubicin (Adriamycin) 20 mg/m2 IV once per day on days 1 to 3
*Etoposide (Vepesid) 150 mg/m2 IV once per day on days 1 to 3
====Supportive therapy====
*Mesna (Mesnex) 3000 mg/m2/day IV continuous infusion over 72 hours, started on day 1 (total dose per cycle: 9000 mg/m2)
21-day cycle for up to 6 cycles
====Subsequent treatment====
*VAI consolidation, then HD with auto HSCT consolidation
===References===
# Strauss SJ, McTiernan A, Driver D, Hall-Craggs M, Sandison A, Cassoni AM, Kilby A, Michelagnoli M, Pringle J, Cobb J, Briggs T, Cannon S, Witt J, Whelan JS. Single center experience of a new intensive induction therapy for Ewing's family of tumors: feasibility, toxicity, and stem cell mobilization properties. J Clin Oncol. 2003 Aug 1;21(15):2974-81. link to original article dosing details in manuscript have been reviewed by our editors EuroPMC
Category:Ewing sarcoma regimens
Category:Disease-specific pages
Category:Bone sarcomas