Pamela Isley (Earth 1)

Pamela Isley is a Gotham City botanist at Gotham Labs until her professor, Dr. Jason Woodrue injects her with poison tranforming her in a villain known as Poison Ivy and an adversary of the superhero known as the Batman.
== Biography ==
Dr. Pamela Lillian Isley, PhD, a Gotham City botanist. She grows up wealthy with emotionally distant parents and later studies advanced botanical biochemistry at a university with Alec Holland under Dr. Jason Woodrue. Isley, a shy girl, is easily seduced by her professor. Woodrue injects Isley with poisons and toxins as an experiment, causing her transformation. She nearly dies twice as a result of these poisonings, driving her insane. Later, Woodrue flees from the authorities leaving Isley in the hospital for six months. Enraged at the betrayal, she suffers from violent mood swings, being sweet one moment and evil the next. When her boyfriend has a car accident after mysteriously suffering from a massive fungal overgrowth, Isley drops out of school and leaves Seattle, eventually settling in Gotham City.
She begins her criminal career by threatening to release her suffocating spores into the air unless the city meets her demands. Batman, who appears in Gotham that very same year, thwarts her scheme, and she is incarcerated in Arkham Asylum.
=== The Long Halloween ===
Ivy joins Carmine Falcone at his son Alberto's funeral. Using her powers, she takes control of Bruce Wayne when he shakes her hand. Ivy uses her control over Bruce to force him to sign over assets over to the Falcones, unintentionally keeping him from investigating the Holiday murders as Batman.
Three months later, Catwoman puts and end to Ivy's manipulations and defeats her, sending her to Arkham Asylum.
On October 31st, The former district attorney, turned supervillain Two-Face and his accomplice Solomon Grundy attacks Arkham and free several inmates; Ivy, Scarecrow, Joker, Penguin and Mad Hatter attack Falcone's building and took his hostage. As they then captured Falcone, Ivy and the others waited for Two-Face to end his life so they could have the city for themselves but Batman and Catwoman entered. During the final battle, Ivy captures both Batman and Catwoman and tries to use her mind-control again, but Batman resists, knocking Ivy out. After the fight had ended, they were all taken back to Arkham Asylum again.
=== The Hangman Murders ===
Once a place controlled by organized crime, Gotham City suddenly finds itself being run by lawless freaks, such as Poison Ivy, Mr. Freeze, and the Joker. It is still early in Batman's crimefighting career, but the Dark Knight is on the verge of completing his transformation into the city's greatest defender.
=== Meeting Harley Quinn ===
During in prison, Isley meets Harley Quinn, The Joker's girlfriend and the two become best friends.
=== Hiding ===
After Harley was dumped by Joker, Ivy escaped from Arkham and hides in the abandoned Wayne Botanical Garden, who is noticeably weaker. She kills anyone who steps foot on sight but only sparing Harley due to how kind she was during her time as her therapist. 
=== HarIvy Team Up ===
Harley starts getting attacked by every criminal that had previously held a grudge against her or hired by Joker. Learning about Joker's bounty, Harley decided that she needs help, she goes to the abandoned Wayne Botanical Garden where her best friend, Poison Ivy was hiding during her escape from Arkham after Harley was dumped. Ivy is noticeably weaker. She kills anyone who steps foot on sight but only sparing Harley due to how kind she was during her time as her therapist. Harley ask Ivy to team up after Joker put a bounty on her, Ivy agreed the team up and take down criminals that want Harley dead.
=== CatHarIvy Team Up ===
Selina Kyle AKa Catwoman has been in a war with Black Mask, she destroys his warehouses and killing his men after he killed Holly Robinson years ago. Ivy and Harley meet her on the roof, who ask her to join them. Selina agreed to join them as The Trio defeated criminals like Victor Zsasz, Penguin, Boneblaster, and Red Tool
=== The First Encounter With Punchline ===
Heard about Harley teaming up with Ivy and Catwoman, Joker hired Punchline, a replacement sidekick to replace Harley and sends her to kill them. Punchline attack and ambush The Trio at a warehouse, where they defeated a group of gangsters. The Trio defeated Punchline and learned about Joker hiring a gang to kill Harley. The Trio leave Punchline to the police and keep fighting Joker's gang.
=== Bar Ambush ===
The Trio went to a bar and have a drink and talk about their lives and origins, but a gang of bar goons attack them for the bounty. The Trio defeated them and left but got captured by The Joker.
=== The Final Fight Against The Joker ===
Joker took The Trio to Amusement Mile and tie them up. Joker wants Harley to live him again but Harley refuses, Joker put a gun on her and order her to love him or he will kill Ivy and Selina. Selina breaks free and freed Harley and Ivy and fight Joker and his gang. In a final showdown on the pier between Harley and Joker. Joker tells Harley that she needs him to survive but Harley refuses and always need friends to back her up and defeated Joker.
=== The Gotham City Sirens ===
In the aftermath, Joker was in by The GCPD and Catwoman continues of taking down Black Mask's operation. Ivy admits Harley is the reason she loves humanity, and when the two kiss Ivy blooms and regains her full strength and the two started dating. If some criminal attacks The Trio, they will be called The Gotham City Sirens while Joker was interviewed by Dr. Jeremiah Arkham about the events.
== Relationships ==
=== Allies ===
* Julian Day / Calendar Man - friend and inmate
* Jonaathan Crane / Scarecrow - friend and inmate
* Oswald Cobblepot / Penguin - friend and inmate
* Jervis Tetch / Mad Hatter - friend and inmate
* Harvey Dent / Two-Face - friend and inmate
* Victor Fries / Mr. Freeze - friend and inmate
* Waylon Jones / Killer Croc - friend and inmate
* Cyrus Gold / Solomon Grundy - friend and inmate
* Edward Nashton / Riddler - friend and inmate
* Kirt Langstrom / Man-Bat - friend and inmate
* Garfield Lynns / Firefly - friend and inmate
* Drury Walker / Killer Moth - friend and inmate
* Gotham City Sirens
** Harleen Quinzel / Harley Quinn - best friend and inmate and girlfriend
** Selina Kyle/Catwoman - enemy now friend
=== Enemies ===
* Bruce Wayne / Batman - enemy
* Dick Grayson / Robin - enemy
* Barbara Gordon / Batgirl - enemy
* Jason Todd / Robin ll † - enemy
* Tim Drake / Robin lll - enemy
* Gotham City Police Department
** James Gordon
** Harvey Bullock
** Renee Montoya
** Janice Porter † - victim
* Carmine Falcone † - Formal Boss and Enemy
* Alberto Falcone/Holiday † - Enemy
* Sofia Falcone/Hangman † - Enemy
* Sal Maroni † - Enemy
* Jason Woodrue - enemy and formal boss and creator
* Jack Oswald White / The Joker - formal friend and inmate now enemy
* Alexis Kaye / Punchline