DNAseq: Ion Torrent

==Overview==
The Ion Torrent technology works by measuring the change in pH after each base is translated. The product is made from three layers: a layer of micro-machined wells, an ion-sensitive layer, and a proprietary ion sensor layer. Each well holds a DNA template. When a nucleotide is added to a template, a hydron is released into the solution which is charged to a specific pH. The change in pH is measured by the ion sensor and translated into digital information. The order of the bases and base letters can be viewed on a screen.
==Uses==
*Microbial Sequencing
*Mitochondrial Sequencing
**"16 samples per run with barcoding"
*Amplicon Sequencing
**This uses PCR-based amplification method. The PCR-based method amplifies regions of the genome by using specific enzymes.
**Good for low-input DNA samples
*Targeted Resequencing
**Uses In-Solution Hybridization-Based Pullout Method
**Good for viewing exons or large sets of genes
**Can be used for "structural variation detection"
*RNA Sequencing
*Validation and Library Assessment
*Exome Sequencing
*Human-Scale Genome Sequencing
*Chromatin Immunoprecipitation Sequencing
*Whole-Transcriptome Sequencing
==Videos==
http://www.youtube.com/watch?v=yVf2295JqUg&feature=related
http://www.youtube.com/watch?v=jv-JHafk4UA&feature=relmfu
==References==
All textual information was acquired from the Life Technologies Website in the Ion Torrent Section: Life Technologies