List of The Powerpuff Girls Characters

== Voice Cast ==
=== The Powerpuff Girls ===
* Blossom (voiced by Cathy Cavadini in seasons 1-17, later by Amanda Leighton in seasons 18 and ongoing) is the self-proclaimed leader of the Powerpuff Girls. Her personality ingredient is "everything nice", her signature color is pink, and she has long red-orange hair with a red bow. She was named for having spoken freely and honestly to the Professor shortly after her creation as shown in The Powerpuff Girls Movie. She is often seen as the most level-headed, and composed member of the group and also strong and determined. Her unique abilities include Ice breath, microscopic vision, lightning bolts, and advanced intelligence; she is also an exceptional leader, master strategist, and apt planner.
* Bubbles (voiced by Tara Strong in the series seasons 1-17, and by Kath Soucie in the What a Cartoon! episodes, later by Kristen Li in seasons 18 and ongoing) is the "softest and sweetest" of the three. Her signature color is blue, her personality ingredient is "sugar", and she has blonde hair in pigtails. Bubbles is seen as kind and very sweet but she is also capable of extreme rage and can fight monsters just as well as her sisters can. Her best friend is a stuffed octopus doll she calls "Octi", and she also loves animals. She exhibits the ability to both understand multiple languages and communicate with various animals; her unique powers are projecting powerful sonic screams, and creating a shockwave of thunder with a single clap from her hands.
* Buttercup (voiced by E. G. Daily in seasons 1-17, later by Natalie Palamides in seasons 18 and ongoing) is described as a "tough hotheaded tomboy". Her personality ingredient is "spice", her signature color is green, and she has short black hair in a flip. She loves to get dirty, fights hard and plays rough; she does not plan and is all action. Her unique powers are too curl her tongue, spinning into a tornado, and creating fireballs by rubbing her hands together 'till smoke comes out which forms a flaming ball that she throws at an opponent. McCracken originally wanted to name the character "Bud" until a friend suggested the name Buttercup.
* Blisstina (voiced by Olivia Olson in seasons 19-21 and by Grace Rolek in seasons 22 and ongoing) - Bliss is the original Powerpuff Girl in the reboot, and is referred to as the fourth one because she is the fourth Powerpuff Girl to be shown in the reboot. She first appears in the five-part special, "The Power of Four". Her hair color is electric blue, and she often wears a pastel blue hairband with a pink heart adorned upon it. In two flashbacks depicting events that occur ten years prior to the main events of the series, Professor Utonium attempts to create the perfect little girl in response to his rival Newtronium creating the perfect little boy; Utonium uses sugar, spice, and everything nice. However, he accidentally adds Chemical W to the concoction, thus creating Bliss. Mojo Jojo also considered himself Bliss' childhood friend when he was the Professor's lab assistant. Bliss possesses superhuman strength, supersonic flight, superhuman hearing, laser vision, and limited invulnerability. However, unlike Blossom, Bubbles and Buttercup, Bliss also possesses psionic abilities, but her powers are uncontrollable due to her emotions. As such, in a flashback scene, Bliss' powers caused the destruction of the Professor's residence after she throws a temper tantrum, and the Professor ends up unconscious. Bliss then flies off to Bird Poop Island to keep everyone safe from her superpowers (Mojo himself was saddened to see Bliss run away), and meets her pet elephant, Mee. After spending several years on Bird Poop Island, Bliss moves back to Townsville; after saving the girls from being beaten by the Gnat, she is considered a hero and gets some training to control her powers. But the Professor dismisses her from ever using her powers again, as he insists that she is not ready yet. However, Mee convinces Bliss to prove him wrong as the girls invite her to defeat Chipmunk Commando. Her anger gets the best of her, as she almost hurts the girls, and Mee reveals that he is in fact Him in disguise. He manipulates her into fusing with him, and causes mayhem in Townsville within Bliss's body. But she then breaks free from Him's body thanks to Mojo Jojo, who tries to get her to notice him but fails. Bliss, Blossom, Bubbles and Buttercup then combine together by the power of sisterhood to defeat Him. Because of the planet Saturn being moved closer to Earth, Bliss leaves to bring it back to its rightful place in the Solar System. She also pledges to come back to see Utonium and the Powerpuffs again when she is done.
* Bunny (voiced by Candi Milo in seasons 1-17 and by JoJo Siwa in seasons 18 and ongoing) - The fifth member of the Powerpuff Girls.
* Bell (voiced by Janice Kawaye in seasons 1-17 and by Ava Acres in seasons 18 and ongoing) - The sixth member of the Powerpuff Girls.
* Bullet (voiced by Nika Futterman in seasons 1-17 and by Francesca Capaldi in seasons 18 and ongoing) -The seventh member of the Powerpuff Girls.
* Banana (voiced by Sandy Fox in seasons 1-17 and by Isabella Acres in seasons and 18 and ongoing) - The eighth member of the Powerpuff Girls.
* Berry (voiced by Ariana Greenblatt) - The ninth member of the Powerpuff Girls.
=== Secondary Characters ===
* Professor Utonium (voiced by Tom Kane in seasons 1-21, later by Beck Bennett in seasons 22 and ongoing) is a scientist who works in his home in the suburbs of the City of Townsville, and the Powerpuff Girls' creator and father. A stereotypical scientist, his general attire is a white lab coat and black pants. The Professor once housed a chimpanzee named Jojo (later to become Mojo Jojo) as a lab assistant who proved to be reckless and destructive. While the Professor was stirring his perfect girl concoction, Jojo shoved him, causing him to accidentally break a flask containing the mysterious "Chemical X" which spilled into the formula, thus creating the Powerpuff Girls. The Professor is a genius in many fields of science, having shown knowledge in fields such as physics, chemistry, and biology as well as being a skilled inventor. Aside from his role as a scientist, he acts as a parent to the Powerpuff Girls. Although he apparently did not legally "adopt" the girls, everyone who addresses him calls him "their father" and they as "his daughters". The Professor can be strict, easily manipulated and overprotective at times, but still retains his caring fatherly image. In the episode "Keen for Keane", the girls set up Professor Utonium and their kindergarten teacher Ms. Keane on a date for Valentine's Day, resulting in the two temporarily becoming an item.
* The Mayor of Townsville (voiced by Tom Kenny in the series seasons 1 and ongoing and by Jim Cummings in the What a Cartoon! episodes) is generally referred throughout only as "Mayor", although he is referred to as "Barney" by his wife in the episode "Boogie Frights". He is short and elderly with a fringe of white hair around a bald scalp and a thick white mustache. He wears a monocle over his left eye and a small top hat that “floats” just above his head. The Mayor is rather dimwitted, but cares deeply about his city. He has a love for pickles, and is very childish at times. He sometimes calls the girls for very silly reasons, as seen in the episode "Bubblevicious" when he calls because of a traffic jam. The What a Cartoon! episode "Meat Fuzzy Lumpkins" featured a different Mayor who was younger and taller than the Mayor of the series.
* Ms. Sara Bellum (voiced by Jennifer Martin in the series seasons 1-7, Jill Talley in seasons 8-17, Jennifer Hale in seasons 18 and ongoing) is the Mayor's secretary, who often handles issues that the Mayor cannot. She has long, curly red hair, wears a red business suit and red high heel shoes for a secretary look. Her face is never fully shown onscreen, although The Powerpuff Girls Rule!!! revealed part of it. Running gags include other onscreen characters often finding her attractive, the camera focusing on her chest, and her face always being obscured. Her name is a pun on the word cerebellum presumably due to her intelligent and wise nature. She also proved to be a capable fighter, battling the villain Sedusa by herself when the girls were disabled. She appeared in one episode of the reboot.
* Ms. Keane (voiced by Jennifer Hale in seasons 1-21 and by Kath Soucie in the What-a-Cartoon! episodes, later by Rachel Bloom in seasons 22 and ongoing) is the kindergarten teacher of Pokey Oaks Kindergarten and later teaches Midway Elementary School. She wears an orange shirt, a red vest, brown pants, and has short black hair and light blue eyes. She is patient, wise, understanding, and protective of her students, making her a well-liked, motherly figure. She is also an animal lover, owning a ginger cat named Valentino, and the class pet hamster, Twiggy. Ms. Keane forbids any fighting in school, even if there is a crisis taking place within it; she convinces the girls that there are other ways to solve problems other than fighting as demonstrated in the episodes "Schoolhouse Rocked" and "Imaginary Fiend".
* Narrator (voiced by Tom Kenny in the series seasons 1 and ongoing and Gary Owens in Whoopass Stew and by Ernie Anderson in the What a Cartoon! episodes) is the enthusiastic but unseen narrator of the show. He often comments on the proceedings of the episode, says things which happen to be hints about the plots of certain episodes, and frequently breaks the fourth wall. He is also shown to be the only connection between the audience and the girls, as he can "talk" to both and even be involved, such as Mojo Jojo transforming him into a dog. In the episode "Simian Says", he is kidnapped by Mojo Jojo, who promptly takes his place as the episode begins.
* Talking Dog (voiced by Tom Kane in the series seasons 1-21 and by Brian Cummings in the What a Cartoon! episodes, later by Patton Oswalt in seasons 22 and ongoing) is a small white dog with black ears and nose and a black spot on his back, wearing a red collar with a yellow dog tag. When he stays with the girls he is shown to be blunt, abrasive and insulting, though his demeanor remains straightforward and earnest. He is frequently abused in almost every appearance he makes; as a running gag, no one ever seems to regard his pain and simply ignores him.
* Mitch Mitchelson (voiced by Tom Kenny in seasons 1 and ongoing): The bully at Pokey Oaks Kindergarten. Mitch has brownish hair, and wears a black T-shirt with the words "MITCH ROCKS" on the front. Usually talks in a gruff voice; he torments the kids in the class in a few episodes, though he is not a major threat. He is Buttercup's close friend, according to Buttercup in her interview in the DVD features of The Powerpuff Girls Movie. For a brief time he hosted a short segment on Cartoon Network called "Mitch Rocks", where he looked at various things and said whether they "rock" or not.
* Robin Snyder (voiced by Julie Nathanson in seasons 5-21 and by Mckenna Grace in seasons 22 and ongoing): Robin is a young little girl who is the next-door neighbor and best friend for life of the Powerpuff Girls.
* The Derbytantes (voiced by Kate Higgins and Jessica DiCicco, respectively) - The Derbytantes are a group of bad girl roller skaters who Buttercup usually likes hanging around with. They never take their skates off and enjoy playing "Deathball" (a sport resembling a cross between dodgeball and Rollerball). Notable members include: Maylyn (the leader, with pink hair and lightning bolts on her uniform), Jaylyn (blue hearts), Haylyn (yellow hearts) and Bobby Susan Ray-Lyn (who looks more like a grown man).
* Donny (voiced by Josh Fadem) - Donny is a young unicorn who is best friends with Bubbles
=== Villains ===
* Mojo Jojo (voiced by Roger L. Jackson) is a mad scientist anthropomorphic chimpanzee with great intelligence, who speaks with a Japanese accent in an overly redundant manner. As the main antagonist of the series and the Powerpuff Girls' archenemy, Mojo Jojo was Professor Utonium's reckless laboratory assistant, Jojo, before Professor Utonium created the Powerpuff Girls, the same accident that created the Girls gave Jojo his super-intelligence. He has green skin, pink scleras, and wears a white glass dome-shaped helmet that covers his oversized, protruding brain. He wears white gloves and boots, a blue suit with a white belt, and a flowing purple cape. Despite his high intelligence, his plans often contain flaws that he overlooks, and he rarely has much success against the girls, as their superpowers are too much for his inventions. However, he is a master at manipulation and trickery, often fooling the girls into thinking he has changed despite their constant encounters. Mojo lives in an observatory atop a dormant volcano located in the middle of Townsville's municipal park.
* HIM (voiced by Tom Kane in seasons 1-21 and by Keith Ferguson in seasons 22 and ongoing) is a mysterious, sinister, androgynous, and supernatural demon. His physical appearance is an amalgamation of sorts with lobster-like claws, red/orange skin, pointed ears, a hooked nose, yellow/green eyes, a long curled beard, wearing makeup, a woman's red jacket and skirt, and black stiletto heel boots. His voice ranges from a high-pitched falsetto to a deep, booming basso profondo. HIM's plans are usually psychological in nature, and often consist of manipulating events to either drive the Powerpuff Girls insane or drive wedges between them. HIM possesses various supernatural and demonic abilities such as shapeshifting, mind manipulation, reality warping, various kinds of energy manipulation, and more. Craig McCracken has stated that the character "HIM" was inspired by the mannerisms of the Chief Blue Meanie in the 1968 film Yellow Submarine. McCracken also stated HIM is his favorite villain.
* Fuzzy Lumpkins (voiced by Jim Cummings) is a pink Bigfoot-like hillbilly monster with a wide jaw, a green nose, two antennas on his head, big blue overalls, and brown boots, who commonly plays a banjo which he calls "Jo." He was the first villain introduced in the series, appearing in the pilot "Meat Fuzzy Lumpkins," in which he turned the people of Townsville into meat after he lost in a jam contest. He speaks in a southern accent, and lives in the woods next to Townsville. Fuzzy has a limited intellectual capacity, and is not nearly so active and ambitious as most of the villains in the series. He usually limits himself to shooting anyone whom he finds trespassing on his property, though also prone to destructive fits of rage; when angry, Fuzzy turns dark red, grows claws, and his teeth become sharp fangs. Fuzzy (according to Professor Utonium's research) belongs to a race of "Lumpkins"; he has three siblings (Furry, Fluffy, and Hairy), three nephews (Buzzy, Wuzzy, and Scuzzy), and several dozen cousins who closely resemble him.
* Princess Morbucks (voiced by Jennifer Hale in seasons 1-17, Haley Mancini in seasons 18-21 and by Dove Cameron in Don't Cry, Blossom only) is a spoiled, rich, and bratty little girl, whose partially unseen and mostly mute father allows her to finance various evil plots to destroy the Powerpuff Girls. Her supervillain outfit consists of a yellow, Powerpuff-style dress, black gloves and boots, and a tiara. Her hatred of the girls stems from her rebuffed attempt to become one of them. Her father is "Daddy" Morbucks, a large, rotund man whose face and head are always hidden from the viewer. He dotes on his daughter and usually indulges in her whims, but it is hinted that his patience is limited and Princess fears truly angering him. Thanks to him, Morbucks has access to virtually unlimited financial resources.
* Ace (voiced by Jeff Bennett): A mean-spirited and opportunistic rogue with a slick-back hairdo, a colored vest-jacket, a peach-fuzz mustache, a pair of shades, and also has fangs. As the Gang's leader and their smartest member, he is the instigator of most of their antisocial activities, and he does possess a certain charismatic charm that allows him to sweet-talk people who ought to know better, such as Ms. Keane in the episode "School House Rocked" and even Buttercup in the episode "Buttercrush". He speaks in a nasally Italian/New Yorker accent. His full name is Ace D. Copular. In 2018, Ace temporarily became the replacement bassist of the virtual band Gorillaz while Murdoc Niccals was in prison.
* Snake (voiced by Tom Kenny in the series): A slippery character with a forked tongue, skinny body, and a hissing voice, Snake is the Gang's second-in-command, but this mostly makes him a yes-man to Ace. He does have a personality of his own, but it is usually silenced due to Ace punching him when he speaks out of line (or asks a foolish question). It is revealed in "School House Rocked" that his real name is Sanford D. Ingleberry.
* Lil' Arturo (voiced by Tom Kenny and by Carlos Alazraqui in his first appearance): A malevolent Mexican midget boy, Lil' Arturo seems to take the most amusement from the Gang's activities. In the episode "School House Rocked" he carries around a switchblade-styled comb he calls "Maria", a treasured gift from his incarcerated father. He has a rather prominent underbite. His full name is Arturo de la Guerra, which is Spanish for "Arthur from the War". His small size allows him to sneak through cramped spaces and passages that the rest of the gang cannot reach.
* Grubber (voiced by Jeff Bennett): A barefoot hunchback with untidy hair, grubby clothes, and protruding eyes. He also appears to be mostly mute, communicating instead through blowing a raspberry. He does, however, occasionally demonstrate unexpected talents, such as playing the violin, speaking eloquently or performing impersonations. When attending the girls' school for a short time, Grubber showed that he was able to horribly contort his body, making him briefly resemble a well-spoken and handsome-looking young man before snapping back to his normal self. His full name is Grubber J. Gribberish.
* Big Billy (voiced by Jeff Bennett): A hulking, dimwitted colossus who acts as the Gang's muscle. Though he does at times display a childlike innocence, he mostly just does the bidding of his more savvy and vindictive friends. In the episode "School House Rocked", it is revealed that his real name is William W. Williams and that he is a cyclops, with his single eye hidden beneath his shaggy red hair. Although he rarely fares any better in combat against the girls than the rest of the gang, he has the muscular power to stop a speeding train from running down citizens from the track and tackle monsters to the ground with his bare hands. He is fiercely loyal to anyone he considers to be a friend, and briefly tried to help the Powerpuff Girls after they saved him from a train. However, his clumsiness and lack of intelligence made him more destructive as a hero than he was as a villain.
* Bossman: (voiced by Chuck McCann in seasons 1-18 and by Rob Paulsen in seasons 19 and ongoing) - The leader and general spokesman of the Amoeba Boys. He wears a gray fedora.
* Junior: (voiced by Chuck McCann in seasons 1-18 and by Maurice LaMarche in seasons 19 and ongoing) - The smallest one of the Amoeba Boys. He wears a black cap and usually repeats whatever Bossman says. In the episode Crime 101, he is named Tiny.
* Slim: (voiced by Chuck McCann in seasons 1-18 and by Cam Clarke in seasons 19 and ongoing) - The tallest one of the Amoeba Boys. He wears a brown fedora and is even slower and more incompetent than the other amoebas. In the episode Crime 101, he is called Skinny Slim.
* Sedusa (voiced by Jennifer Hale in the series in seasons 1-17 and by Catherine Tate in seasons 18 and ongoing)) is a young, beautiful Gorgon-like mistress of disguise and seductress who uses her feminine wiles to influence men to do her bidding, such as Professor Utonium, the Mayor, and the Gangreen Gang. When her identity is revealed, she typically doffs down to her leotard battle suit and fights with her prehensile hair. Her eyes also change color depending on her mood. When she is acting benevolent her eye color is green, but when she is angry, revealing her true villainous nature, her eyes turn red.
* Packrat (voiced by Jason Spisak) - Packrat is a scheming, oversized rat from the sewers. He loves stealing "shiny things" from stores and the people of Townsville, which he sees as gifts for various dolls he views as girlfriends. He broke Bubbles' arm in "Strong Armed". In "Little Octi Lost", he stole Octi from Buttercup.
* Allegro (voiced by Eric Bauza) - Allegro is a happy party-obsessed, panda-like being who is capable of causing people to go into a stupor of pure happiness. He is very similar to Panda from We Bare Bears, another Cartoon Network TV show. His true form is a smaller blue bear. He is actually one half of an entity called the Cosmic Bear.
* Manboy (voiced by Maurice LaMarche) - Manboy is a short man with super strength and a magical beard (similar to Sedusa's hair), and has a tall wood figure that he uses in battle. He is an enemy to the Powerpuff Girls, mainly to Buttercup, because he is incredibly sexist and constantly declares men are superior to women. He is eventually revealed to be a child who gained an adult body when he got his powers.
* Barbarus and Bianca Bikini (voiced by Lily Vonnegut and Natalie Palamides, respectively) - Bianca and Barbarus Bikini are a stylish human and gorilla, respectively. Bianca was initially chosen to be the new assistant of the Mayor, before being found out. They have an offshore hideout.
* Jemmica (voiced by Anais Fairweather) - Jemmica is a thief who presents herself as a treasure-hunting adventurer but had actually stolen artifacts from archeological digs. It is revealed in "Total Eclipse of the Kart" that she is actually ancient evil Jemoire, Queen of the Storms.
* Silico (voiced by Jason Spisak) - The mysterious villain Silico first appears in the episode "Viral Spiral", where he offers the Amoeba Boys a way to destroy the internet and finally become big-name villains. In "Halt and Catch Silico", he uses a social media news web site Muck Bucket to post false news stories about the Powerpuff Girls. The Powerpuff Girls confront him in his lair and he reveals that he was originally a lonely kid who built toy robot friends to keep him company, however they were inadvertently destroyed when a monster the Powerpuff Girls defeated fell on his house, causing him to swear revenge against the Powerpuff Girls who he held responsible due to their carelessness. The Powerpuff Girls manage to defeat him when he unwisely calls Blossom "unorganized", but it turns out to be a hologram he created. In "Take Your Kids to Dooms Day", he steals a power suit to fight the Powerpuff Girls before being defeated by the Professor. In "The Trouble with Bubbles", he hacks a robotic clone of Bubbles to destroy the Powerpuff Girls, but Robo-Bubbles sacrifices herself to defeat him before she explodes. He survives an explosion and disguises as the Professor to replace him. In the Episode "Lights Out!", he disguises as Bliss and gave everyone in Townsville, the Butley. He shuts down the power, and mind controlled everyone in Townsville to antagonize and destroy the Powerpuff Girls, even Bliss too. But was stopped by Bubbles by giving him a modified Butley, he appears to drop a bomb on the girls and leaves the library to explode. Later this, he was escorted to jail for his crimes.
* Janitaur (Voiced by: Jeff Bennett) - Wendell Finestein is the janitor of Midway Elementary School. He calls himself Janitaur after an accident during a science fair when he was a kid turned him to a Centaur.
* Gnat (Voiced by Jason Kaye) - A mutated gnat who considers himself the girls' oldest enemy. His mutation came from being bitten by an old man who was irradiated from being stupid enough to stare into an open microwave.
* Largo (Voiced by: Eric Bauza) - Another bear-like entity who is the exact opposite of Allegro and the other half of the Cosmic Bear.
* DupliKate (Voiced by: Jill Talley) - A villainess with the ability to make copies of whatever her powers are used on.
* Sporde (Voiced by: Ben Diskin)- An alien outlaw who Bliss was hunting down. Briefly disguised himself as a human agent named Logan Logan. He is eventually put in a jar after Bliss defeats him.
* Lester Van Luster (Voiced by: Dwight Schultz) - A evil magician who shrunk Townsville.
* Stanicorn (Voiced by: Maurice LaMarche) - An evil unicorn who was the main antagonist of the second season premiere "The Last Donnycorn".
=== The Rowdyruff Boys ===
* Brick (voiced by Rob Paulsen in the series seasons 1 and ongoing): Blossom's equivalent, he is the short-tempered, self-proclaimed leader of the Rowdyruff Boys. He has an abrasive and rash attitude, which often causes conflict with his brothers. He wears a backwards red baseball cap compared to Blossom's bow. He also originally had bangs, which were replaced with long spiky hair when he was revived.
* Boomer (also voiced by Paulsen in the series seasons 1 and ongoing): Bubbles' equivalent, he is a loud-mouthed dunce, as opposed to Bubbles' naïve sweetness, and has been known to like disgusting things. After his resurrection, Boomer is shown to be the least intelligent of the boys and he is often bullied and bossed around by his brothers. Boomer's hair parts in a curved fashion like Bubbles', but is longer and spiky on each end, in a wings haircut.
* Butch (voiced by Roger L. Jackson in the series seasons 1 and ongoing): Buttercup's equivalent. After his resurrection, Butch becomes more hyperactive and overly excited. Butch's hair is spiked upwards, abandoning his previous cowlick from his first appearance. He also shares Buttercup's small triangular hair parting.
* Blaze (voiced by Ogie Banks): The fourth member of the Rowdyruff Boys.
* Blitz (voiced by Sean Giambrone): The fifth member of the Rowdyruff Boys.
* Blaine (voiced by Atticus Shaffer): The sixth member of the Rowdyruff Boys.
* Boost (voiced by Joshua Rush): The seventh member of the Rowdyruff Boys.
* Bolt (voiced by Antonio Raul Corbo): The eighth member of the Rowdyruff Boys.
=== Live-action segment characters ===
=== Scientists ===
* Scientist Sally (portrayed by Blossom's voice actress, Cathy Cavadini) - The host of the series, a live-action scientist and the president of the Powerpuff Girls Fan Club.
* Robot Ralph (voiced by Sandy Fox) - Sally sidekick robot.
* Scientist Carly (portrayed by Bubbles' voice actress, Tara Strong)
* Scientist Jane (portrayed by Buttercup's voice actress, E.G. Daily)
* Scientist Phyllis (portrayed by Bunny's voice actress, Candi Milo)
* Scientist Dot (portrayed by Bell's voice actress, Janice Kawaye)
* Scientist Nicole (portrayed by Bullet's voice actress, Nika Futterman)
* Scientist Tracy (portrayed by Banana's voice actress, Sandy Fox)
* Scientist Laura (portrayed by Blossom's current voice actress, Amanda Leighton)
* Scientist Jessica (portrayed by Bubbles' current voice actress, Kristen Li)
* Scientist Mina (portrayed by Buttercup's current voice actress, Natalie Palamides)
* Scientist Evelyn (portrayed by Blisstina's original voice actress, Olivia Olson)
* Scientist Becca (portrayed by Blisstina's current voice actress, Grace Rolek)
* Scientist Fallon (portrayed by Bunny's current voice actress, JoJo Siwa)
* Scientist Diamond (portrayed by Bell's current voice actress, Ava Acres)
* Scientist Chelsea (portrayed by Banana's current voice actress, Isabella Acres)
* Scientist Jackie (portrayed by Bullet's current voice actress, Francesca Capaldi)
* Scientist Samantha (portrayed by Berry's current voice actress, Ariana Greenblatt)
=== Other Humans ===
* Mr. Labbenson (portrayed by Professor Utonium's voice actor, Tom Kane)
* Dr. Kenny (portrayed by The Mayor of Townsville's voice actor, Tom Kenny)
* Dr. Rebecca (portrayed by Ms. Bellum's second voice actress, Jill Talley)
* Linda (portrayed by Ms. Bellum's voice actress, Jennifer Martin)
* Wendy Workalot (portrayed by Ms. Keane's voice actress, Jennifer Hale)
* Emily Enderson (portrayed by Robin Snyder's voice actress, Julie Nathanson)
* Gregory (portrayed by Mojo Jojo's voice actor, Roger L. Jackson)
* Scientist Sally's Dad (voiced by The Gangreen Gang's voice actor, Jeff Bennett)
* Ben (portrayed by Fuzzy Lumpkins voice actor, Jim Cummings)
* Professor Parkingpants (portrayed by The Amoeba Boys' voice actor, Chuck McCann)
* Roger (portrayed by Butch and Boomer's voice actor, Rob Paulsen)
* Painter Pierre (portrayed by Professor Utonium's current voice actor, Beck Bennett)
* Traveling Tanya (portrayed by Ms. Keane's current voice actress, Rachel Bloom)
* Minnie and Stephanie (portrayed by the Derbynates' voice actresses, Kate Higgins and Jessica DiCicco)
* Detective Dawson (portrayed by Donny's voice actor, Josh Fadem)
* Shelton (portrayed by HIM's current voice actor, Keith Ferguson)
* Lindsay (portrayed by Princess Morbucks's current voice actress, Haley Mancini)
* Officer Oliver (portrayed by Lil' Arturo's voice actor, Carlos Alazraqui)
* Rachel the Royal Guard (portrayed by Sedusa's current voice actress, Catherine Tate)