Southern Railway

== Asia ==
*Southern Railway Zone (India)
*Railway to Beersheba, Israel
== Europe ==
*Austrian Southern Railway, a railway company operating in Austria-Hungary between 1841 and 1923
*South railway (Austria)
*Southern (Great Britain Current), operator of the South Central rail franchise in the United Kingdom since 2000
*Southern Railway (Great Britain), a railway company in southern England between 1923 and 1947
*Southern Railways (Ukraine)
*Southern Railway (Württemberg)
==North America==
*Norfolk Southern Railway, a Class 1 railroad in the eastern United States
**Southern Railway (U.S.) between 1894 and 1982, now part of Norfolk Southern
***Georgia Southern Railroad
***Southern Railway (Indiana)
***Southern Railway (Kentucky)
***Southern Railway in Mississippi
***Tennessee and Carolina Southern Railway
*Canada Southern Railway, part of the New York Central Railroad
*Southern Railway and Navigation Company, a line of steamboats on the Ohio and Tennessee Rivers
*Arizona Southern Railroad
*Arkansas Southern Railroad, part of the Chicago, Rock Island and Pacific Railway
*British Columbia Southern Railway, part of the Canadian Pacific Railway
*Southern Railway of British Columbia
*California Southern Railroad (possibly several companies of that name)
*Carolina Southern Railway
*Dakota Southern Railroad, part of the Chicago, Milwaukee, St. Paul and Pacific Railroad
*Dakota Southern Railway
*Florida Southern Railroad and Florida Southern Railway, part of the Atlantic Coast Line Railroad
*Illinois Southern Railway, part of the Missouri Pacific Railroad
*Indiana Southern Railroad (never built)
*Indiana and Illinois Southern Railway, part of the Illinois Central Railroad
*Iowa Southern Railroad, part of the Wabash Railway
*Iowa Southern Railway, part of the Chicago and North Western Railway
*Kansas Southern Railway, part of the Atchison, Topeka and Santa Fe Railway
*Southern Railroad of Long Island, part of the Long Island Rail Road
*Louisiana Southern Railway, part of the Missouri Pacific Railroad
*Southern Railroad (Michigan), part of the New York Central Railroad
*Minnesota Southern Railway, part of the Chicago, Milwaukee, St. Paul and Pacific Railroad
*Southern Railway and Light Company and Southern Railway and Traction Company, a street railway in Natchez, Mississippi
*Missouri Southern Railroad
*Southern Railway (St. Louis), a street railway in St. Louis, Missouri
*Montana Southern Railway
*Nebraska Southern Railway, part of the Missouri Pacific Railroad
*Nevada Southern Railroad, part of the Atchison, Topeka and Santa Fe Railway
*New Brunswick Southern Railway, part of the Canadian Pacific Railway
*New Jersey Southern Railroad and New Jersey Southern Railway, part of the Central Railroad of New Jersey
*Ohio Southern Railroad (disambiguation)
*Pennsylvania Southern Railroad
*Quebec Southern Railway (disambiguation)
*Southern Railroad of New Jersey, a short line
*Tennessee Southern Railroad, part of the Illinois Central Railroad
*Texas Southern Railway
*Southern Railway (Texas), part of the Atchison, Topeka and Santa Fe Railway
*Utah Southern Railroad (disambiguation)
*Vermont Southern Railroad, part of the Boston and Maine Railroad
*Virginia and Carolina Southern Railroad, part of the Atlantic Coast Line Railroad
*Virginia Southern Railroad
*Washington Southern Railway (Virginia) in Virginia, south from Washington, D.C.
*West Virginia Southern Railway, part of the Norfolk and Western Railway
==South America==
*PeruRail, formerly known as Ferrocarril del Sur (Peru Southern Railway)