niryuu/sni-each-converted huggingface dataset:
Facioscapulohumeral dystrophy (FSHD) is characterized by chromatin relaxation of the D4Z4 macrosatellite array on chromosome 4 and expression of the D4Z4-encoded DUX4 gene in skeletal muscle. The more common form, autosomal dominant FSHD1, is caused by contraction of the D4Z4 array, whereas the genetic determinants and inheritance of D4Z4 array contraction-independent FSHD2 are unclear. Here, we show that mutations in SMCHD1 (encoding structural maintenance of chromosomes flexible hinge domain containing 1) on chromosome 18 reduce SMCHD1 protein levels and segregate with genome-wide D4Z4 CpG hypomethylation in human kindreds. FSHD2 occurs in individuals who inherited both the SMCHD1 mutation and a normal-sized D4Z4 array on a chromosome 4 haplotype permissive for DUX4 expression. Reducing SMCHD1 levels in skeletal muscle results in D4Z4 contraction-independent DUX4 expression. Our study identifies SMCHD1 as an epigenetic modifier of the D4Z4 metastable epiallele and as a causal genetic determinant of FSHD2 and possibly other human diseases subject to epigenetic regulation.
Output: What is the mode of inheritance of Facioscapulohumeral muscular dystrophy (FSHD)?
Yeliz Fndk (born in 1976) is a Turkish female martial artist competing in the Taekwondo, boxing, Muay Thai, Kickboxing and wushu discipilines.
Output: What is the gender Yeliz Fındık is associated with?
Parkinson's disease is the most common neurodegenerative movement disorder. -Synuclein is a small synaptic protein that has been linked to familial Parkinson's disease (PD) and is also the primary component of Lewy bodies, the hallmark neuropathology found in the brain of sporadic and familial PD patients. The function of -synuclein is currently unknown, although it has been implicated in the regulation of synaptic vesicle localization or fusion. Recently, overexpression of -synuclein was shown to cause cytoplasmic vesicle accumulation in a yeast model of -synuclein toxicity, but the exact role -synuclein played in mediating this vesicle aggregation is unclear. Here, we show that -synuclein induces aggregation of many yeast Rab GTPase proteins, that -synuclein aggregation is enhanced in yeast mutants that produce high levels of acidic phospholipids, and that -synuclein colocalizes with yeast membranes that are enriched for phosphatidic acid. Significantly, we demonstrate that -synuclein expression induces vulnerability to perturbations of Ypt6 and other proteins involved in retrograde endosome-Golgi transport, linking a specific trafficking defect to -synuclein phospholipid binding. These data suggest new pathogenic mechanisms for -synuclein neurotoxicity.
Output: Which is the primary protein component of Lewy bodies?
Sinlap is a circular depression on Titan, a natural satellite of Saturn.
Output: Where is Sinlap found?
Ossy Renardy was killed in a road accident on 3 December 1953, aged only 33.
Output: What cause of death was listed for Ossy Renardy?
Ehlers-Danlos syndromes (EDS) and Marfan syndrome (MFS) are multisystemic disorders that primarily affect the soft connective tissues. Both disorders have benefited from recent advances in clinical and molecular characterization, allowing improvements in clinical diagnosis and management. EDS are a heterogeneous group of conditions characterized by skin hyperextensibility, atrophic scarring, joint hypermobility and generalized tissue fragility. The current classification proposes six subtypes based on clinical, biochemical and molecular characteristics. However, examples of unclassified variants and 'overlap phenotypes' are becoming more common. Mutations in genes encoding fibrillar collagens or collagen-modifying enzymes have been identified in most forms of EDS, including the classic and vascular subtypes (collagen type V and III, respectively), and the rare arthrochalasis, kyphoscoliosis and dermatosparaxis variants (type I collagen defects). To date, the genetic background of the hypermobility type of EDS remains unclear, although some new insights have been gained recently. MFS is an autosomal-dominant disorder that affects the cardiovascular, ocular and skeletal system with aortic root dilation/dissection, ectopia lentis and bone overgrowth, respectively. Advances in therapeutic, mainly surgical, techniques have improved median survival significantly, yet severe morbidity and a substantial risk for premature mortality remain associated. The disorder is caused by mutations in the FBN1 gene, encoding the microfibrillar protein fibrillin-1. Recently, new insights in the pathogenesis changed the prevailing concept of this type 1 fibrillinopathy as a structural disorder of the connective tissue into a developmental abnormality manifesting perturbed cytokine signalling. These findings have opened new and unexpected targets for aetiologically directed drug treatments.
Output: What tissue is most affected in Ehlers-Danlos syndromes?
Magnetic resonance imaging (MRI) has a higher sensitivity and specificity (90% and 79%) than plain radiography (54% and 68%) for diagnosing diabetic foot osteomyelitis. MRI performs somewhat better than any of several common tests--probe to bone (PTB), erythrocyte sedimentation rate (ESR) >70 mm/hr, C-reactive protein (CRP) >14 mg/L, procalcitonin >0.3 ng/mL, and ulcer size >2 cm--although PTB has the highest specificity of any test and is commonly used together with MRI. No studies have directly compared MRI with a combination of these tests, which may assist in diagnosis.
Output: Which disease can be diagnosed with the "probe to bone" test?
Mary Ball was born in 1812, the second daughter of Bob Stawell Ball and his wife Mary nee Green.
Output: Who was the male parent of Mary Ball?
AtxA, a unique regulatory protein of unknown molecular function, positively controls expression of the major virulence genes of Bacillus anthracis. The 475 amino acid sequence of AtxA reveals DNA binding motifs and regions similar to proteins associated with the phosphoenolpyruvate: carbohydrate phosphotransferase system (PTS). We used strains producing native and functional epitope-tagged AtxA proteins to examine protein-protein interactions in cell lysates and in solutions of purified protein. Co-affinity purification, non-denaturing polyacrylamide gel electrophoresis and bis(maleimido)hexane (BMH) cross-linking experiments revealed AtxA homo-multimers. Dimers were the most abundant species. BMH cross-links available cysteines within 13 A. To localize interaction sites, six AtxA mutants containing distinct CysSer substitutions were tested for multimerization and cross-linking. All mutants multimerized, but one mutation, C402S, prevented cross-linking. Thus, BMH uses C402 to make the inter-molecular bond between AtxA proteins, but C402 is not required for protein-protein interaction. C402 is in a region bearing amino acid similarity to Enzyme IIB proteins of the PTS. The AtxA EIIB motif may function in protein oligomerization. Finally, cultures grown with elevated CO(2) /bicarbonate exhibited increased AtxA dimer/monomer ratios and increased AtxA activity, relative to cultures grown without added CO(2) /bicarbonate, suggesting that this host-associated signal enhances AtxA function by shifting the dimer/monomer equilibrium towards the dimeric state.
Output: Which metabolite activates AtxA?
The plasma membrane Na(+)/Ca(2+) exchanger (NCX) is a bidirectional ion transporter that couples the translocation of Na(+) in one direction with that of Ca(2+) in the opposite direction. This system contributes to the regulation of intracellular Ca(2+) concentration via the forward mode (Ca(2+) efflux) or the reverse mode (Ca(2+) influx). We have previously demonstrated that the Ca(2+) paradox, an in vitro reperfusion model, causes the sustained activation of the reverse mode of the NCX, the disruption of Ca(2+) homeostasis, and subsequent delayed apoptotic-like death in astrocytes. In addition, we found that the nitric oxide (NO)-cyclic GMP signaling pathway inhibits Ca(2+) paradox-mediated astrocyte apoptosis, while a high concentration of NO induces cytotoxicity. In this way, Ca(2+) and NO may work together in the pathogenesis of several cells in the central nervous system. Concerning the role of NCX in NO cytotoxicity, we have found, using the specific inhibitor of NCX 2-[4-[(2,5-difluorophenyl)methoxy]phenoxy]-5-ethoxyaniline (SEA0400), that NCX is involved in NO-induced cytotoxicity in cultured microglia, astrocytes, and neuronal cells. This review summarizes the pathological roles of the NCX as a new target for NO-mediated cellular toxicity, based on our studies on NO-NCX-mediated glial toxicity.
Output: The small molecule SEA0400 is an inhibitor of which ion antiporter/exchanger?
Viktor Zubkov (Chairman, Russian Special Presidential Representative for Cooperation with Gas Exporting Countries Forum, First Deputy Prime Minister of Russia, former Prime Minister of Russia) Alexey Miller (Deputy Chairman, Chairman of the Management Committee, CEO, Chairman of Gazprombank, former Deputy Minister of Energy of Russia) Andrey Akimov (Chairman of Gazprombank) Farit Gazizullin (former Minister of State Property of Russia, former Minister of Property Relations of Russia) Timur Kulibaev (Chairman of Legal Entities Department) Vitaly Markelov (Deputy Chairman of the Management Committee) Viktor Martynov (Rector of Gubkin Russian State University of Oil and Gas, Professor) Vladimir Mau (Rector of the Russian Presidential Academy of National Economy and Public Administration) Valery Musin (Head of the Civil Procedure Department, Faculty of Law, Saint Petersburg State University) Alexander Novak (Minister of Energy of the Russian Federation) Mikhail Sereda (Deputy Chairman of the Management Committee, Head of the Administration of the Management Committee of Gazprom) Former members of the board:
Output: Who led Gazprom?
Sir Arthur Gore, 2nd Baronet (c. 1685 -- 10 February 1742) was an Irish politician and baronet.
Output: What was the title that Sir Arthur Gore, 2nd Baronet held?
British author JK Rowling was at the release of her latest Harry Potter book called "Harry Potter and the Deathly Hallows" at the Natural History Museum in London, Friday July 20, 2007. J.K. Rowling has been spotted at cafes in Scotland working on a detective novel, a British newspaper reported Saturday. The Sunday Times newspaper quoted Ian Rankin, a fellow author and neighbor of Rowling's, as saying the creator of the "Harry Potter" books is turning to crime fiction. "My wife spotted her writing her Edinburgh criminal detective novel," the newspaper quoted Rankin as telling a reporter at an Edinburgh literary festival. "It is great that she has not abandoned writing or Edinburgh cafes," said Rankin, who is known for his own police novels set in the historic Scottish city. Rowling famously wrote initial drafts of the Potter story in the Scottish city's cafes. Back then, she was a struggling single mother who wrote in cafes to save on the heating bill at home. Now she's Britain's richest woman - worth $1 billion, according to Forbes magazine - and her seven Potter books have sold more than 335 million copies worldwide. In an interview with The Associated Press last month, Rowling said she believed she was unlikely to repeat the success of the Potter series, but confirmed she had plans to work on new books. "I'll do exactly what I did with Harry - I'll write what I really want to write," Rowling said.
Output: What is JK Rowling famous for?
Doctors have a new weapon in the battle against obesity -- a talking plate that tells people not to eat too quickly. The Mandometer monitors the amount of food leaving the plate, and tells users, "Please eat more slowly." The PS1,500 Swedish device is to be used in a National Health Service plan to help hundreds of obese families lose weight. It comes in two parts -- a scale placed under the plate and a small computer screen showing a graphic of the food gradually disappearing as the user eats. A red line on the screen shows the user's eating speed, while a blue line shows a healthy rate. If the user eats too fast, the red line angles away from the blue one, warning him or her to _ . If the lines deviate too much, the computer voice comes on, and the screen flashes the message "Are you feeling full yet?" to remind users to think about whether they have had enough. Britain has an increasingly serious obesity problem, with one in four adults and one in seven children classed as obese. After a recent trial using the device, experts believe teaching obese people to eat more slowly will help them know when they are full. Around 600 families with at least one obese parent and child (aged five or older) were targeted in the project by Bristol University, along with GPs and nurses. Professor Julian Hamilton-Shield, who is leading the plan, says obese children and adolescents using the Mandometer ate from 12 to 15 percent less per meal at the end of the 12-month trial. Six months after they stopped using the device they still ate less, and continued to lose weight. "It will be a powerful tool to help families retrain their eating habits," he says.
Output: What does the red line on the screen show?
Captain Nazi was introduced into the modern DC Universe in Jerry Ordway's The Power of Shazam! series in 1995.
Output: Which fictional universe is Captain Nazi from?
Quarrels between Denmark and Sweden led to the Northern Seven Years' War in 1563, which ended in 1570 with the Treaty of Stettin. Primarily fought in western and southern Scandinavia, the war involved important naval battles fought in the Baltic. When Danish-held Varberg surrendered to Swedish forces in 1565, 150 Danish mercenaries escaped the subsequent massacre of the garrison by defecting to Sweden. Among them was Pontus de la Gardie, who thereafter became an important Swedish commander in the Livonian War. Livonia was also affected by the naval campaign of Danish admiral Peter or Per Munck, who bombarded Swedish Reval from sea in July 1569. The Treaty of Stettin made Denmark the supreme and dominating power in Northern Europe, yet failed to restore the Kalmar Union. Unfavourable conditions for Sweden led to a series of conflicts that only ended with the Great Northern War in 1720. Sweden agreed to turn over her possessions in Livonia in return for a payment by Holy Roman Emperor Maximilian II. Maximilian failed to pay the promised compensation, however, and thereby lost his influence on Baltic affairs. The terms of the treaty regarding Livonia were ignored, and thus the Livonian War continued. From Ivan's point of view, the treaty enabled the powers involved to form an alliance against him, now that they were no longer fighting each other.
Output: Which was first, the Northern Seven Years' War or the Great Northern War?
The focus of this article is acrokeratosis paraneoplastica, one of two disorders that have acquired the eponym Bazex syndrome. To date, all of the patients reported in the literature have had an underlying neoplasm, most commonly squamous cell carcinoma of the upper aerodigestive tract. In this review of 113 cases of acrokeratosis paraneoplastica (mean age, 61 years; 105 males, 8 females), the psoriasiform lesions preceded the diagnosis of the associated malignancy in 73 (67%) of 109 patients, whereas the cutaneous manifestations followed the diagnosis of the neoplasm in only 16 (15%) of 109; in the remainder, the onset of the skin lesions and the diagnosis of the tumor occurred simultaneously. Therefore, awareness of the cutaneous signs of Bazex syndrome is of obvious importance to dermatologists. Evidence in favor of the paraneoplastic nature of this disease is as follows: in 81 (93%) of 87 patients with adequate clinical descriptions, the skin lesions either improved significantly (or resolved) when the underlying neoplasm was treated or they remained unchanged in the setting of persistent disease. Occasionally, the reappearance of skin lesions has signaled a recurrence of the tumor.
Output: Name synonym of Acrokeratosis paraneoplastica.
An Alexanderson alternator is a rotating machine invented by Ernst Alexanderson in 1904 for the generation of high-frequency alternating current for use as a radio transmitter.
Output: Who found Alexanderson alternator?
Ruby Hunter died of a heart attack on 17 February 2010, aged 54.
Output: Due to which disease did Ruby Hunter die?
Birgul Sadkoglu (born March 23, 2000) is a Turkish women's football midfielder currently playing in the Turkish Women's First Football League for Eskisehirspor with jersey number 20.
Output: What  is Birgül Sadıkoğlu's position on the field while playing football?
Sunday Reed (born Lelda Sunday Baillieu) (15 October 1905 -- 15 December 1981) was notable for supporting and collecting Australian art with her husband John Reed.
Output: What is Sunday Reed's spouse's name?
Does everyone want a challenging job? In spite of all the attention focused by the media, academicians, and social scientists on human potential and the needs of individuals, there is no evidence to support that the vast majority of workers want challenging jobs. Some individuals prefer highly complex and challenging jobs; others develop in simple, routine work. The individual-difference variable that seems to gain the greatest support for explaining who prefers a challenging job and who doesn't is the strength of an individual's needs for personal growth and self-direction at work. Individuals with these higher-order growth needs are more responsive for challenging work. What percentage of ordinary workers actually desire higher-order need satisfactions and will respond positively to challenging jobs? No current data is available, but a study from the 1970s estimated the figure at about 15%. Even after adjusting for changing work attitudes and the growth in white-collar jobs, it seems unlikely that the number today exceeds 40%. The strongest voice advocating challenging jobs has not been workers--it's been professors, social science researchers, and media people. Professors, researchers, and journalists undoubtedly made their career choices, to some degree, because they wanted jobs that gave them autonomy, recognition and challenge. That, of course, is their choice. But for them, to force their needs onto the workforce in general is presumptuous . Not every employee is looking for a challenging job. Many workers meet their higher-order need off the job. There are 168 hours in every individual's week. Work rarely consumes more than 30% of this time. That leaves considerable opportunities, even for individuals with strong growth needs, to find higher-order need satisfaction outside the workplace. So don't feel you have a responsibility to create challenging jobs for all your employees. For many people, work is something that will never excite or challenge them. And they don't expect to find their growth opportunities at work. Work is merely something they have to do to pay their bills. They can find challenges outside of work on the golf course, fishing, at their local pub, with their friends in social clubs, with their family, and the like.
Output: What makes people choose challenging jobs?
Joy Tivy was born in Carlow, Ireland in 1924 and she commenced studies at the University College Dublin in 1942 where she studied geography as her primary subject with botany and geology as her secondary areas.
Output: What is the name of university that educated Joy Tivy?
Epigenetic modifications of histones regulate gene expression and chromatin structure. Here we show that Meisetz (meiosis-induced factor containing a PR/SET domain and zinc-finger motif) is a histone methyltransferase that is important for the progression of early meiotic prophase. Meisetz transcripts are detected only in germ cells entering meiotic prophase in female fetal gonads and in postnatal testis. Notably, Meisetz has catalytic activity for trimethylation, but not mono- or dimethylation, of lysine 4 of histone H3, and a transactivation activity that depends on its methylation activity. Mice in which the Meisetz gene is disrupted show sterility in both sexes due to severe impairment of the double-stranded break repair pathway, deficient pairing of homologous chromosomes and impaired sex body formation. In Meisetz-deficient testis, trimethylation of lysine 4 of histone H3 is attenuated and meiotic gene transcription is altered. These findings indicate that meiosis-specific epigenetic events in mammals are crucial for proper meiotic progression.
Output: What is the characteristic domain of histone methyltransferases?
Selenocysteine (Sec, U) insertion into proteins is directed by translational recoding of specific UGA codons located upstream of a stem-loop structure known as Sec insertion sequence (SECIS) element. Selenoproteins with known functions are oxidoreductases containing a single redox-active Sec in their active sites. In this work, we identified a family of selenoproteins, designated SelL, containing two Sec separated by two other residues to form a UxxU motif. SelL proteins show an unusual occurrence, being present in diverse aquatic organisms, including fish, invertebrates, and marine bacteria. Both eukaryotic and bacterial SelL genes use single SECIS elements for insertion of two Sec. In eukaryotes, the SECIS is located in the 3' UTR, whereas the bacterial SelL SECIS is within a coding region and positioned at a distance that supports the insertion of either of the two Sec or both of these residues. SelL proteins possess a thioredoxin-like fold wherein the UxxU motif corresponds to the catalytic CxxC motif in thioredoxins, suggesting a redox function of SelL proteins. Distantly related SelL-like proteins were also identified in a variety of organisms that had either one or both Sec replaced with Cys. Danio rerio SelL, transiently expressed in mammalian cells, incorporated two Sec and localized to the cytosol. In these cells, it occurred in an oxidized form and was not reducible by DTT. In a bacterial expression system, we directly demonstrated the formation of a diselenide bond between the two Sec, establishing it as the first diselenide bond found in a natural protein.
Output: What is the name of the stem loop present in the 3' end of genes encoding for selenoproteins?
The APP/PS1ki mouse model for Alzheimer's disease (AD) exhibits robust brain and spinal cord axonal degeneration and hippocampal CA1 neuron loss starting at 6 months of age. It expresses human mutant APP751 with the Swedish and London mutations together with two FAD-linked knocked-in mutations (PS1 M233T and PS1 L235P) in the murine PS1 gene. The present report covers a phenotypical analysis of this model using either behavioral tests for working memory and motor performance, as well as an analysis of weight development and body shape. At the age of 6 months, a dramatic, age-dependent change in all of these properties and characteristics was observed, accompanied by a significantly reduced ability to perform working memory and motor tasks. The APP/PS1ki mice were smaller and showed development of a thoracolumbar kyphosis, together with an incremental loss of body weight. While 2-month-old APP/PS1ki mice were inconspicuous in all of these tasks and properties, there is a massive age-related impairment in all tested behavioral paradigms. We have previously reported robust axonal degeneration in brain and spinal cord, as well as abundant hippocampal CA1 neuron loss starting at 6 months of age in the APP/PS1ki mouse model, which coincides with the onset of motor and memory deficits described in the present report.
Output: Which disease the London mutation involved in?
GODI is a package management system for the OCaml programming language.
Output: What is the programming language for GODI?
Bloodstream infections with Staphylococcus aureus are clinically significant and are often treated with empirical methicillin resistance (MRSA, methicillin-resistant S. aureus) coverage. However, vancomycin has associated harms. We hypothesized that MRSA screening correlated with resistance in S. aureus bacteremia and could help determine the requirement for empirical vancomycin therapy. We reviewed consecutive S. aureus bacteremias over a 5-year period at two tertiary care hospitals. MRSA colonization was evaluated in three ways: as tested within 30 days of bacteremia (30-day criterion), as tested within 30 days but accounting for any prior positive results (ever-positive criterion), or as tested in known-positive patients, with patients with unknown MRSA status being labeled negative (known-positive criterion). There were 409 S. aureus bacteremias: 302 (73.8%) methicillin-susceptible S. aureus (MSSA) and 107 (26.2%) MRSA bacteremias. In the 167 patients with MSSA bacteremias, 7.2% had a positive MRSA test within 30 days. Of 107 patients with MRSA bacteremia, 68 were tested within 30 days (54 positive; 79.8%), and another 21 (19.6%) were previously positive. The 30-day criterion provided negative predictive values (NPV) exceeding 90% and 95% if the prevalence of MRSA in S. aureus bacteremia was less than 33.4% and 19.2%, respectively. The same NPVs were predicted at MRSA proportions below 39.7% and 23.8%, respectively, for the ever-positive criterion and 34.4% and 19.9%, respectively, for the known-positive criterion. In MRSA-colonized patients, positive predictive values exceeded 50% at low prevalence. MRSA screening could help avoid empirical vancomycin therapy and its complications in stable patients and settings with low-to-moderate proportions of MRSA bacteremia.
Output: What is MRSA?
Delamanid (OPC-67683), a nitro-dihydro-imidazooxazole derivative, is a new antituberculosis medication that inhibits mycolic acid synthesis and has shown potent in vitro and in vivo activity against drug-resistant strains of Mycobacterium tuberculosis. In this randomized, placebo-controlled, multinational clinical trial, we assigned 481 patients (nearly all of whom were negative for the human immunodeficiency virus) with pulmonary multidrug-resistant tuberculosis to receive delamanid, at a dose of 100 mg twice daily (161 patients) or 200 mg twice daily (160 patients), or placebo (160 patients) for 2 months in combination with a background drug regimen developed according to World Health Organization guidelines. Sputum cultures were assessed weekly with the use of both liquid broth and solid medium; sputum-culture conversion was defined as a series of five or more consecutive cultures that were negative for growth of M. tuberculosis. The primary efficacy end point was the proportion of patients with sputum-culture conversion in liquid broth medium at 2 months. Among patients who received a background drug regimen plus 100 mg of delamanid twice daily, 45.4% had sputum-culture conversion in liquid broth at 2 months, as compared with 29.6% of patients who received a background drug regimen plus placebo (P=0.008). Likewise, as compared with the placebo group, the group that received the background drug regimen plus 200 mg of delamanid twice daily had a higher proportion of patients with sputum-culture conversion (41.9%, P=0.04). The findings were similar with assessment of sputum-culture conversion in solid medium. Most adverse events were mild to moderate in severity and were evenly distributed across groups. Although no clinical events due to QT prolongation on electrocardiography were observed, QT prolongation was reported significantly more frequently in the groups that received delamanid. Delamanid was associated with an increase in sputum-culture conversion at 2 months among patients with multidrug-resistant tuberculosis. This finding suggests that delamanid could enhance treatment options for multidrug-resistant tuberculosis. (Funded by Otsuka Pharmaceutical Development and Commercialization; ClinicalTrials.gov number, NCT00685360.).
Output: Which disease can be treated with Delamanid?
Something strange was unbelievable. Take Robert for example. After the terrible car accident, his whole world had been completely dark and quiet for almost ten years. The loss of sight and hearing made him try many times to put end to his life. His family especially his wife did their best to tend and comfort him. By and by he finally regained the courage to live on. On a hot summer afternoon he was taking a walk with a stick near his house when a thunderstorm started suddenly. He stood under a large tree in order not to get himself wet. Unfortunately he was struck down to the ground by a lightning. The witnesses thought him dead but he woke up some 20 minutes later lying face down in muddy water below the tree. He felt that he was trembling badly, but when he opened his eyes ,he didn't dare to believe that he saw a plough( )lying near the wall. When Mrs Edwards came running up to him, she shouted their neighbours for help. And he saw her and heard her voice for the first time in nearly ten years. The news of Robert's regaining his sight and hearing quickly spread in his area. And many doctors came to prove the truth of the news. Most of them said that he gained sight and hearing again obviously from the knock of lightning; none of them could give convincing reasons, however. The only reasonable explanation given by one doctor was that, since Edwards lost his sight and hearing as a result of sudden shock in a terrible accident, perhaps the only way for them to regain was by another sudden shock.
Output: What did Edwards see first after being struck down by a lightning?
Air Antilles Express is a French airline based at Pointe-a-Pitre International Airport in Guadeloupe.
Output: At what airport can you find Air Antilles Express?
A dolly is a machine because it changes a force to make work easier. What is work? In physics, work is defined as the use of force to move an object over a distance. It is represented by the equation: Work = Force x Distance All machines make work easier, but they dont increase the amount of work that is done. You can never get more work out of a machine than you put into it. In fact, a machine always does less work on an object than the user does on the machine. Thats because a machine must use some of the work put into it to overcome friction. Friction is the force that opposes motion between any surfaces that are touching. All machines involve motion, so they all have friction. How much work is needed to overcome friction in a machine depends on the machines efficiency. Efficiency is the percent of work put into a machine by the user (input work) that becomes work done by the machine (output work). The output work is always less than the input work because some of the input work is used to overcome friction. Therefore, efficiency is always less than 100 percent. The closer to 100 percent a machines efficiency is, the better it is at reducing friction. Look at the ramp in the Figure 1.1. A ramp is a type of simple machine called an inclined plane. It is easier to push the heavy piece of furniture up the ramp to the truck than to lift it straight up off the ground, but pushing the furniture over the surface of the ramp creates a lot of friction. Some of the force applied to moving the furniture must be used to overcome the friction with the ramp. Q: Why would it be more efficient to use a dolly to roll the furniture up the ramp? A: There would be less friction to overcome if you used a dolly because of the wheels. So the efficiency of the ramp would be greater with the dolly. Efficiency can be calculated with the equation: Output work Efficiency = Input work 100% Consider a machine that puts out 6000 joules of work. To produce that much work from the machine requires the user to put in 8000 joules of work. To find the efficiency of the machine, substitute these values into the equation for efficiency: 6000 J 100% = 75% 8000 J Q: Rani puts 7500 joules of work into pushing a box up a ramp, but only 6700 joules of work actually go into moving the box. The rest of the work overcomes friction between the box and the ramp. What is the efficiency of the ramp? A: The efficiency of the ramp is: 6700 J 100% = 90% 7500 J
Output: the percent of the work put into a machine (input work) that is actually used to do work (output work) is a measure of the machines
To study the mechanism of centrosome duplication in cycling cells, we established a novel system of multiple centrosome formation in two types of cells: CHO cells treated with RO3306, a Cyclin-dependent kinase 1 (Cdk1) inhibitor and DT40 cells, in which Cdks were knocked out by chemical genetics. Cdk1-inactivated cells initiated DNA replication and centrosome duplication at the onset of S phase. They became arrested at the end of G2, but the centrosome cycle continued to produce supernumerary centrioles/centrosomes without DNA endoreplication in those cells. Centrosomes were amplified in a highly synchronous and reproducible manner: all of them were located next to the nucleus and spread widely apart from each other with several m in distance. Double knockout of Cdk1 and Cdk2 caused cell cycle arrest at G1/S and centrosomes were no longer duplicated. However, cells continued to grow and increased their volume over 10-fold during 48 hr of culture. Centrosome components, including -tubulin and Cep135, were synthesized and accumulated during the arrest, allowing rapid centrosome multiplication upon recovery from the cell cycle arrest or expression of exogenous Plk4 in G1/S cells. Thus centrosome amplification results from the discoordination of the centrosome cycle from the progression of other cell cycle events, which is controlled by different levels of Cdk activities.
Output: Where in the cell do we find the protein Cep135?
Uhtred the Bold and Eadwulf Cudel were sons of Waltheof, earl of Bamburgh, who died in 1006.
Output: Who was the brother of Eadwulf Cudel?
Empirical data have challenged chronic posttraumatic stress disorder (PTSD) consisting of three dimensions. In the present study we aimed to determine the factor structure of acute posttraumatic symptoms in two recently traumatized samples. In sample 1, 203 civilian trauma survivors were administered the Davidson Trauma Scale (DTS) approximately 1 week posttrauma. In sample 2, 182 civilian treatment seeking trauma survivors completed the DTS at an average of 41.4 days posttrauma. Our confirmatory factor analyses indicated that a 4-factor intercorrelated model provided the best representation of the data in both samples. The four factors are best described as reexperiencing, active avoidance, dysphoria, and hyperarousal. For acute posttraumatic symptoms, the empirical data suggest to split the avoidance cluster into 'Active avoidance' and 'Dysphoria'-confirming findings in studies on chronic PTSD. In future revisions of the DSM, the diagnostic criteria for PTSD may need to be adapted to fit the research findings.
Output: Symptoms of which disorder are evaluated with the Davidson Trauma Scale?
Saltburn, Marske and New Marske is a civil parish in Redcar and Cleveland in north east England.
Output: What was replaced Saltburn, Marske and New Marske?
Monument with Standing Beast is a sculpture by Jean Dubuffet in front of the Helmut Jahn designed James R. Thompson Center in the Loop community area of Chicago, Illinois.
Output: What is the name of the place where Monument with Standing Beast can be found?
Originating in Germany, the Danube flows southeast for 2,860 km (1,780 mi), passing through or touching the border of Austria, Slovakia, Hungary, Croatia, Serbia, Romania, Bulgaria, Moldova and Ukraine before emptying into the Black Sea.
Output: What body of water does Danube join?
In the computer age, most of us take a broadband Internet connection for granted. Whether cable or mobile broadband, today's connections are many times faster than those in the early days. Videos play smoothly, complex websites load quickly, and files download much faster than ten years ago. And the files are much bigger too. There's _ , of course: You have to live near enough to a major city to get broadband Internet. If you don't, it's slow dial-up access for you. And for those living really far out, there may be no Internet access at all. Technology experts often talk about the "last mile" problem, which refers to the difficulty of bringing Internet access to remote locations. In cities and suburbs, it's relatively easy to provide access for everyone. It is much more difficult to deliver access to those living far from cities, especially in developing countries. Internet service providers, for their part, have been reluctant to provide access to sparsely populated areas. The handful of Internet users they would reach wouldn't cover the expense. But every year, technological advancements allow of more and more Internet users. Most broadband connections today run over existing cable TV and telephone lines although these technologies aren't available everywhere. Some companies have delivered the Internet over standard power lines. Advancements in cheaper, more efficient fiber optics cables promise to bring extremely fast Internet connections to more users. Still, the "last mile" problem remains hard to deal with. There will always be somewhere that doesn't have an affordable broadband connection. But someday that might not matter. If the rapid progress in cell phone technology is any indication , it may not be too long before an Internet connection simply follows you wherever you go.
Output: What may eventually settle the "last mile" problem?
MicroRNAs (miRNAs) are 21 nt RNAs that regulate many biological processes in plants by mediating translational inhibition or cleavage of target transcripts. Arabidopsis mutants defective in miRNA biogenesis have overlapping and highly pleiotropic phenotypes including serrated leaves and ABA hypersensitivity. Recent evidence indicates that miRNA genes are transcribed by RNA polymerase II (Pol II). Since Pol II transcripts are capped, we hypothesized that CBP (cap-binding protein) 20 and 80 may bind to capped primary miRNA (pri-miRNA) transcripts and play a role in their processing. Here, we show that cbp20 and cbp80 mutants have reduced miRNA levels and increased pri-miRNA levels. Co-immunoprecipitation experiments revealed that pri-miRNAs 159, 166, 168 and 172 could be associated with CBP20 and CBP80. We found that CBP20 and CBP80 are stabilized by ABA by a post-translational mechanism, and these proteins are needed for ABA induction of miR159 during seed germination. The lack of miR159 accumulation in ABA-treated seeds of cbp20/80 mutants leads to increased MYB33 and MYB101 transcript levels, and presumably higher levels of these positive regulators result in ABA hypersensitivity. Genetic and molecular analyses show that CBP20 and 80 have overlapping function in the same developmental pathway as SE and HYL1. Our results identify new components in miRNA biogenesis.
Output: Which polymerase transcribes pri-miRNAs?
HMS R11 was laid down on 1 December 1917 by Cammell Laird at Birkenhead, launched on 16 March 1918 and commissioned on 8 August 1919.
Output: Which corporation was HMS R11 created by?
With an ageing population and improving cancer therapies, the two most common benign and malignant bone diseases, osteoporosis and bone metastases, will continue to affect an increasing number of patients. Our expanding knowledge of the molecular processes underlying these conditions has resulted in novel bone targets that are currently being explored in clinical trials. Clearly, the approval of denosumab, a monoclonal antibody directed against RANKL, has just marked the beginning of a new era for bone therapy with several additional new therapies lining up for clinical approval in the coming years. Potential agents targeting the osteoclast include cathepsin K, currently in phase 3 trials, and src inhibitors. Amongst anabolic agents, inhibitors of the Wnt-inhibitor sclerostin and dickkopf-1 are promising in clinical trials. Here, we will provide a comprehensive overview of the most promising agents currently explored for the treatment of bone diseases.
Output: To the ligand of which receptors does Denosumab (Prolia) bind?
The calcium-sensing receptor (CaSR) is a G-protein-coupled receptor that plays an essential role in maintaining calcium homeostasis. In the present study, we analyzed the CaSR gene in a Korean family with familial hypocalciuric hypercalcemia (FHH). Genetic studies were performed by direct sequence analysis of the CaSR gene in genomic DNA obtained from peripheral leukocytes. A novel heterozygous G to T substitution at nucleotide position 1711 in exon 6, resulting in the G571W mutation, was identified in the CaSR gene in a 26-year-old female with asymptomatic hypercalcemia, a low calcium/creatinine clearance ratio, and normal intact parathyroid hormone. To study CaSR expression, the mutation was introduced by site-directed mutagenesis into a wild-type (WT) CaSR-expressing pCR3.1 vector, and COS-7 cells were transfected with either the WT or mutant CaSR-containing vector. Transfected cells loaded with Fura-2/AM, a fluorescent indicator of Ca
Output: What is the function of calcium-sensing receptor (CaSR)?
The CRISPR/Cas system in prokaryotes provides resistance against invading viruses and plasmids. Three distinct stages in the mechanism can be recognized. Initially, fragments of invader DNA are integrated as new spacers into the repetitive CRISPR locus. Subsequently, the CRISPR is transcribed and the transcript is cleaved by a Cas protein within the repeats, generating short RNAs (crRNAs) that contain the spacer sequence. Finally, crRNAs guide the Cas protein machinery to a complementary invader target, either DNA or RNA, resulting in inhibition of virus or plasmid proliferation. In this article, we discuss our current understanding of this fascinating adaptive and heritable defense system, and describe functional similarities and differences with RNAi in eukaryotes.
Output: Gene silencing can be achieved by RNA interference (RNAi) in eukaryotic organisms. What is the name of the analogous process in prokaryotic organisms?
An Australian inventor has designed and built an environmentally friendly ferry that uses solar and wind power to transport people around Sydney Harbor. Robert Dane and his Solar Sailor were given the top prize at the Australian Design Awards recently. The Solar Sailor has been in use for six months. It uses four sources of energy for its power -- solar, wind, battery power with stored solar energy and a fuel generator in store all controlled by computers. The ferry can travel up to 7.5 knots on just wind and solar power. It's solar wings and fiberglass solar panels which not only take in sunlight and store it in batteries, but also act as sails. "We angle our solar panels to the sun in two planes , which increases the amount of energy we get from the sun by 40 percent," Dane says. "And also we can use that same structure to do another job, which is also the sail, and so these wing sails actually push the ferry forward just like a soft sail does." Computers check the sun and wind and angle the solar wings to take in the most sunlight. If there isn't much wind or it is a cloudy day, energy stored in the batteries runs the electric motor for up to five hours. "If the batteries are too low, then the computer turns the generator on so the boat is always able to meet a commercial schedule, or get from point A to point B no matter what," Dan explains. Dane got his idea for the Solar Sailor from a book on insects. He was amazed how insects use their wings to collect solar energy to warm themselves. "When I read that, I realized there was a good example in nature for what we were going to do, which was to use a solar wing to collect solar energy and also to sail," he says.
Output: What is the Solar Sailor?
H2A.Z is a highly conserved variant of histone H2A with well-characterized roles in transcriptional regulation. We previously reported that H2A.Z and Mcd1, a subunit of the cohesin complex, regulate the establishment of transcriptional silencing at telomeres in Saccharomyces cerevisiae and that H2A.Z broadly dissociated from chromatin during the anaphase-to-telophase transition, coincident with the dissociation of Mcd1 from chromosomes and dissolution of cohesion. In this study, we show that depletion of H2A.Z causes precocious loss of sister chromatid cohesion in yeast without loss of Mcd1 from chromosomes. H2A.Z is deposited into chromatin by the SWR1 complex and is subject to acetylation of its four N-terminal tail lysine residues by the NuA4 and SAGA histone acetyltransferase complexes. We found that cells compromised for function of the SWR1 complex were defective in cohesion, as were cells expressing a form of H2A.Z not subject to acetylation. Finally, inactivation of H2A.Z in metaphase-blocked cells led immediately to cohesion defects, suggesting a direct role for H2A.Z in the maintenance of sister chromatid cohesion.
Output: Which protein mediates the replacement of H2A by H2A.Z in the yeast Saccharomyces cerevisiae?
Mowat-Wilson syndrome (MWS) is a rare mental retardation-multiple congenital anomalies syndrome associated with typical facial dysmorphism. Patients can show a variety of other anomalies like short stature, microcephaly, Hirschsprung disease, malformations of the brain, seizures, congenital heart defects and urogenital anomalies. Mutations leading to haploinsufficiency of the ZFHX1B gene have been described as the underlying cause of this condition. We report on the clinical findings in a 2(1/2)-year-old boy with some aspects out of the MWS-spectrum in addition to unusual anomalies and a novel missense mutation in the ZFHX1B gene.
Output: Which gene is responsible for the development of the Mowat-Wilson syndrome?
Myotonic dystrophy type 2 (DM2) is an autosomal dominant, multisystem disorder caused by a CCTG tetranucleotide repeat expansion located in intron 1 of the zinc finger protein 9 gene (ZNF9 gene) on chromosome 3q 21.3. To describe the clinical, electrophysiologic and pathologic findings in patients with myotonic dystrophy 2. We evaluated 10 patients genetically, clinically and electrophysiologically during the years 2007 to 2008. All patients were of Jewish European ancestry. Among affected individuals, eight patients had symptoms of proximal muscle weakness, two had muscle pain, and two exhibited myotonia. On physical examination six patients had severe weakness of hip flexor muscles. Seven individuals underwent cataract surgery, and cardiac involvement was seen in one case. On the initial electromyographic (EMG) examination five patients demonstrated myotonic discharges; repeated studies showed these discharges in nine cases. Six muscle biopsies showed non-specific pathological changes. Seven patients had an affected first-degree relative with either a diagnosed or an undiagnosed muscular disorder consistent with an autosomal dominant trait. DM2 may often present with proximal muscle weakness without myotonia. EMG may initially fail to show myotonic discharges, but these discharges may eventually show in most cases on repeated EMG. Thus, DM2 may be underdiagnosed and should be included in the differential diagnosis of adult patients of Jewish European ancestry presenting with proximal lower limb weakness.
Output: How is myotonic dystrophy inherited?