niryuu/sni-each-converted huggingface dataset:
Today's drivers may feel shocked by the high price of petrol when they drive to the gas station. However, the car industry has the technology to solve _ . It's the hybrid car . What is a hybrid car? Any car that uses two or more sources of power is a hybrid car. Most hybrid cars on the road right now are petrol-electric hybrids. The petrol-electric hybrid car is just what it sounds like -- a cross between a petrol-powered car and an electric car. A gas-powered car has a fuel tank , which supplies petrol to the engine. An electric car, on the other hand, has a set of batteries that provides electricity for the car. To be useful to you or me, a car should be able to run at least 300 miles (483 km) before refueling, be capable of being refueled quickly and easily and fast enough to keep up with the other traffic on the road. A petrol car meets these requirements but produces a large amount of pollution. An electric car, however, produces almost no pollution, but can only go 50 to 100 miles (80 to 161 km) between recharging . And the problem has been that the electric car is very slow and inconvenient to recharge. A petrol-electric car combines the advantages of the two power sources into one system that uses both gas power and electric power. Some experts believe that the hybrid car is "the next generation of smart cars". A hybrid car can go up to 50% further than a traditional car can on the same amount of gas! It saves driver's money on gas and cuts air pollution!
Output: How many sources of power do most hybrid cars use?
Progress M-10M (Russian: -10), identified by NASA as Progress 42 or 42P, is a Progress spacecraft which was launched on 27 April 2011 to resupply the International Space Station.
Output: On what date was Progress M-10M launched?
Lake Champlain Chocolates (LCC) is a privately held chocolate manufacturer located in Burlington, Vermont, USA.
Output: What is the city where Lake Champlain Chocolates is from?
Mowat-Wilson syndrome (MWS) is a recently delineated mental retardation (MR)-multiple congenital anomaly syndrome, characterized by typical facies, severe MR, epilepsy, and variable congenital malformations, including Hirschsprung disease (HSCR), genital anomalies, congenital heart disease (CHD), and agenesis of the corpus callosum (ACC). It is caused by de novo heterozygous mutations or deletions of the ZFHX1B gene located at 2q22. ZFHX1B encodes Smad-interacting protein-1 (SMADIP1 or SIP1), a transcriptional corepressor involved in the transforming growth factor-beta signaling pathway. It is a highly evolutionarily conserved gene, widely expressed in embryological development. Over 100 mutations have been described in patients with clinically typical MWS, who almost always have whole gene deletions or truncating mutations (nonsense or frameshift) of ZFHX1B, suggesting that haploinsufficiency is the basis of MWS pathology. No obvious genotype-phenotype correlation could be identified so far, but atypical phenotypes have been reported with missense or splice mutations in the ZFHX1B gene. In this work we describe 40 novel mutations and we summarize the various mutational reports published since the identification of the causative gene.
Output: Which gene is responsible for the development of the Mowat-Wilson syndrome?
Reperfusion injury to tissue following an ischemic event occurs as a consequence of an acute inflammatory response that can cause significant morbidity and mortality. Components of both the innate (complement, immunoglobulin, monocytes, and neutrophils) and adaptive (B and T lymphocytes) immune systems have been demonstrated to mediate tissue injury. Spleen tyrosine kinase (Syk) is responsible for membrane-mediated signaling in various cell types including B lymphocytes, macrophages, and T cells. We investigated the ability of a small drug Syk inhibitor, R788, to protect mice against mesenteric ischemia-reperfusion (I/R)-induced local (intestine) and remote lung injury. Mice were fed with chow containing a Syk inhibitor for 6 days before the performance of intestinal I/R, which resulted in silencing of the expression of the active phosphorylated Syk. Syk inhibition significantly suppressed both local and remote lung injury. The beneficial effect was associated with reduced IgM and complement 3 deposition in the tissues and significant reduction of polymorphonuclear cell infiltration. Our data place Syk upstream of events leading to the binding of natural antibodies to the ischemia-conditioned tissues and urge the consideration of the use of Syk inhibitors in the prevention or improvement of tissue injury of organs exposed to ischemia or hypoperfusion.
Output: Which enzyme is inhibited by a drug fostamatinib?
In a double-blind placebo-controlled prospective clinical trial we studied the efficacy and safety of the benzodiazepine antagonist, flumazenil. In 23 patients admitted to the Intensive Care Unit with coma due to overdose with benzodiazepines or other sedatives, flumazenil i.v. (up to 2 mg or placebo) was given. In 13 patients given flumazenil the Glasgow Coma Scale (GCS) increased significantly from 4.9 to 7.8 (p less than 0.05). Six of these 13 patients, including mainly benzodiazepine mono-intoxications, needed only one series of injections (up to 1.0 mg flumazenil); the GCS increased thereby from 4.5 to 10.7 within a maximum of 5 min (p less than 0.01). In the remaining 7 patients, needing two series of injections of flumazenil (up to 2.0 mg), GCS did not rise significantly and coma was related to intoxications with nonbenzodiazepine sedatives, flunitrazepam and in one patient, encephalitis. In the 10 patients receiving placebo, the GCS did not change. A significant increase in the GCS from 5.5 to 10.8 (p less than 0.001) was, however, observed when flumazenil (up to 1.0 mg) was given after placebo. In patients with EEG monitoring the changes in waveform pattern paralleled the clinical response. Effects could be detected within 1-2 min after flumazenil injection and lasted up to 45 min. There were no adverse reactions or benzodiazepine withdrawal symptoms. We conclude that flumazenil is an effective and safe drug in the treatment of benzodiazepine overdose. The use of flumazenil is of diagnostic value in mixed-drug intoxications or coma of unknown origin and is of therapeutic importance for reversal of benzodiazepine intoxications.
Output: Which drug should be used as an antidote in benzodiazepine overdose?
Only a Mother (Swedish: Bara en mor) is a 1949 Swedish drama film directed by Alf Sjoberg.
Output: Who was responsible for the direction of Only a Mother?
9134 Encke (4822 P-L) is an asteroid-belt asteroid discovered on 24 September 1960 by Cornelis Johannes van Houten and Ingrid van Houten-Groeneveld at Leiden University, on photographic plates from the Palomar Observatory.
Output: What is the year 9134 Encke discovered?
SketchFighter 4000 Alpha is a scrolling shooter with a focus on exploration for Mac OS X. The game was created by Lost Minds together with Ambrosia Software.
Output: Who was the publisher of SketchFighter 4000 Alpha?
Protective protein/cathepsin A (PPCA) has a serine carboxypeptidase activity of unknown physiological function. We now demonstrate that this protease activity triggers the degradation of the lysosome-associated membrane protein type 2a (lamp2a), a receptor for chaperone-mediated autophagy (CMA). Degradation of lamp2a is important because its level in the lysosomal membrane is a rate-limiting step of CMA. Cells defective in PPCA show reduced rates of lamp2a degradation, higher levels of lamp2a and higher rates of CMA. Restoration of PPCA protease activity increases rates of lamp2a degradation, reduces levels of lysosomal lamp2a and reduces rates of CMA. PPCA associates with lamp2a on the lysosomal membrane and cleaves lamp2a near the boundary between the luminal and transmembrane domains. In addition to the well-studied role of PPCA in targeting and protecting two lysosomal glycosidases, we have defined a role for the proteolytic activity of this multifunctional protein.
Output: Which is the receptor for substrates of Chaperone Mediated Autophagy?
It was in 1865 that two Frenchmen were looking into the future. Edouard de Laboulaye, who studied history, admired the United States greatly. Frederic Auguste Bartholdi was a famous artist. De Laboulaye was thinking about 1876, when the country he respected so much would celebrate its 100th birthday. What gift would be good enough for the people of France to give? Right away, Bartholdi thought of a giant statue. Bartholdi kept thinking about a statue for several years. Then, in 1871, he visited the United States and saw the perfect place for a huge statue. It was Bedloe's Island in New York. The project -- the Statue of Liberty -- got started almost immediately, and Bartholdi went home to France to begin work. However, the statue was not finished in time for the 100th birthday. The United States Congress approved the use of Bedloe's Island on February 22, 1877. During the 100th celebration in 1876, the arm of the statue that would hold the torch was shown in Philadelphia. In 1879 the huge head was put on display in France. For the face of the statue, Bartholdi used his mother as a model. The statue was finally completed in 1884. The work had been difficult enough, but then some really hard work began. The statue had to be taken apart and packed into boxes. It was not until May 1885 that the statue -- in pieces -- left France for its new home. The statue arrived in June. However, the Americans fell behind on their part of the project. They hadn't raised enough money for building the base of the statue. People wondered if the country really needed or wanted such a gift. Finally, though, the money was raised and the base was completed. The last piece of the statue was put in place on October 28, 1886.
Output: How many years did it take for the Statue of Liberty to be put in place on its base after it was completed?
Ehlers-Danlos syndrome is a complex hereditary connective tissue disorder that is characterized by abnormalities of the skin and joints and visceral and neurological manifestations. At present, at least 11 forms are recognized on the basis of their clinical characteristics, methods of transmission, and biochemical defect. The neurologic manifestations include cerebrovascular disease, peripheral neuropathy, plexopathy, periventricular subependymal heterotopias, and epilepsy. Previously, 2 females were reported to be affected with subependimal periventricular heterotopias and Ehlers-Danlos syndrome type 1. The authors report a new case of a 12-year-old girl with similar clinical and neuroradiological features.
Output: What tissue is most affected in Ehlers-Danlos syndromes?
This study examined the accuracy of the 17-item Dutch version of the Davidson Trauma Scale (DTS) and the four-item SPAN (Startle, Physiological Arousal, Anger and Numbness) to detect survivors at risk for posttraumatic stress disorder (PTSD) within the first 2 weeks after the trauma. 203 civilian survivors of recent trauma with relatively mild symptoms completed the DTS a mean of 8.7 days after experiencing trauma. SPAN scores were computed from the DTS. At a mean of 64.6 days posttrauma, 160 respondents were assessed for diagnosis of PTSD with the Structured Interview for PTSD. Receiver operating characteristic curves showed that the DTS showed good overall screening accuracy (84%). At a cut-off value of 64, the DTS demonstrated a sensitivity of 0.86, a specificity of 0.70, a positive predictive value (PPV) of 0.12, and a negative predictive value (NPV) of 0.98. Overall accuracy of the SPAN was good (89%). At a cut-off of 10 the SPAN showed a sensitivity of 0.86, a specificity of 0.86, a PPV of 0.22, and a NPV of 0.98. The low PPVs were possibly due to the low of prevalence of PTSD in our sample (4.4%). This study shows that both the DTS and the SPAN are comparably accurate in screening early trauma survivors at risk for developing PTSD. The very brief four-item SPAN may be preferred over the longer 17-item DTS especially in settings in which time and resources are limited. Future studies should aim to cross-validate these results in random samples.
Output: Symptoms of which disorder are evaluated with the Davidson Trauma Scale?
Don Quijote cabalga de nuevo (English: Don Quijote Rides Again) is a 1973 Spanish-Mexican comedy film directed by Roberto Gavaldon based on Miguel de Cervantes's novel Don Quixote, starring Cantinflas as Sancho Panza, Fernando Fernan Gomez as Don Quixote, and Maria Fernanda D'Ocon as Dulcinea.
Output: Which is the basis of Don Quijote cabalga de nuevo?
Miklos Ajtai (born 2 July 1946) is a computer scientist at the IBM Almaden Research Center, USA.
Output: What is Miklós Ajtai's place of employment?
Week 10 featured the top-ranked defenses (by yardage against) in each conference, as the Lions hosted the Dolphins. Detroit got off to a quick start. Matt Prater kicked a 26-yard field goal on the opening drive of the game. Later in the first quarter, Calvin Johnson, playing in his first game since Week 5, caught a 49-yard TD pass from Matthew Stafford for a 10-0 lead. Miami managed a 23-yard Caleb Sturgis field goal before halftime, making the score 10-3. The Dolphins took the lead in the third, starting with a 50-yard Stugis field goal. Next, Earl Mitchell blocked Prater's 42-yard field goal attempt, and Dion Jordan returned the ball to the Lions 3-yard line. On the next play, Ryan Tannehill connected with Mike Wallace for the touchdown, putting Miami ahead, 13-10. Prater made good on a 50-yard field goal early in the fourth quarter to knot the score at 13-13, but Sturgis' 20-yarder put Miami back on top by 3. As in the previous two games, the Lions had to rally on their final drive to win the game. Starting at their own 26 with 3:13 left in the game, the drive culminated with Stafford's 11-yard touchdown pass to Theo Riddick, putting the Lions ahead for good, 20-16. This was the third straight game in which the Lions trailed at the two-minute warning, then went ahead on their final drive. It was the second time in a month that Miami had lost a game in the final seconds, following a Week 7 loss to Green Bay in which the final touchdown was scored with 3 seconds remaining. According to STATS Inc., this is the first time Detroit has started a season 7-2 since 1993.
Output: Which player threw the last touchdown pass of the game?
To develop clinical rules for the safe and effective use of flumazenil in suspected benzodiazepine overdose. We assembled a retrospective series of 35 consecutive comatose patients admitted between October 1992 and July 1993 to a toxicologic ICU with the presumptive diagnosis of drug overdose. These patients were divided into two groups. Group A (low-risk) patients had a clinical picture compatible with uncomplicated benzodiazepine intoxication (calm, without abnormalities in pulse or blood pressure, lateralizing signs, hypertonia, hyperreflexia, or myoclonus) in the absence of predefined electrocardiographic or clinical signs of tricyclic antidepressant or other proconvulsant overdose, and absence of an available history of long-term benzodiazepine treatment or an underlying seizure disorder. Group B ("non-low risk") comprised all other patients. Efficacy of flumazenil was categorized as complete awakening (with normal level of alertness), partial awakening, or no change in alertness level. The safety of flumazenil was defined on the basis of the absence of seizures or death. In group A (n=4), flumazenil was associated with complete awakening in three patients and partial awakening in one. No seizures were observed. In group B (n=31), flumazenil was associated with complete awakening in 4 patients, partial awakening in 5, and no response in 22. In group B, five seizures occurred. Comatose patients with clinical or ECG criteria thought to contraindicate the use of flumazenil have a reasonably high risk of seizures after administration of this drug. Low-risk patients may be able to receive flumazenil safely, but they may be only a small portion of comatose patients with suspected overdose.
Output: Which drug should be used as an antidote in benzodiazepine overdose?
Spotlight at Salem Academy and College Academic Camp: Creative Writing, Environmental Education/ Science, Language Camp Address 500 Salem Avenue Winston-Salem, North Carolina 27101 USA (800) 833-1753 Salem spotlight is designed to arouse intellectual curiosity with emphasis on the experience and development of the individual... (more) Get more information about Spotlight at Salem Academy and College Caribbean Summer Sea Camp Academic Camp: Environmental Education/ Science Camp Address Off-Season Address Box 37 North Coast Road PO Box 1461 Little Cayman, BWI Princeton, New Jersey Cayman Islands 08542 USA (609) 933-4559 The Caribbean Summer Sea Camp is a one-week adventure on Little Cayman Island. Campers dive or snorkel to explore the wonders of coral reefs, and view the diverse life in the sea around the island. Coastal Camp at Headlands Institute Academic Camp: Environmental Education/ Science Camp Address Bldg. 1033 Golden Gate National Recreation Area Sausalito, California 94965 USA (415) 332-6961 The Coastal Camp at Headlands Institute combines marine and coastal ecology themes with hands-on educational games and exciting opportunities for discovery in the great outdoors. By promoting environmental work, Coastal Camp allows kids to become responsible earth citizens. Swift Nature Camp Academic Camp: Environmental Education/ Science Camp Address Off-Season Address W7471 Ernie Swift Rd 25 Baybrook Minong, Wisconsin 54859 Oak Brook, Illinois USA 60523 (715) 466-5666 (630) 654-8036 Located in the Northwoods of Wisconsin on a 1,500-acre lake, this small camp offers hands-on nature studies. Self accomplishment is promoted while competition takes a back seat: Horses, Swimming, Scuba, Skiing, Sailing, Canoeing, Arts & Crafts and much more.
Output: Alice, a girl of 10, hopes to learn more about ecology by playing games. Which telephone number should she dial?
Imperial Fields is a football stadium in Morden, south London that is home to Tooting & Mitcham United F.C. and formerly Chelsea L.F.C..
Output: What team uses Imperial Fields?
On 19 April 1918, Detachment Brandenstein took over the town of Lahti. The German troops advanced from the east-southeast via Nastola, through the Mustankallio graveyard in Salpausselka and the Russian garrisons at Hennala. The battle was minor but strategically important as it cut the connection between the western and eastern Red Guards. Local engagements broke out in the town and the surrounding area between 22 April and 1 May 1918 as several thousand western Red Guards and Red civilian refugees tried to push through on their way to Russia. The German troops were able to hold major parts of the town and halt the Red advance. In total, 600 Reds and 80 German soldiers perished, and 30,000 Reds were captured in and around Lahti.
Output: Where did the German troops advance through first, Hennala or Nastola?
The efficacy of the BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) assay was assessed by analyzing nasal swabs and swabs from other body sites for the presence of MRSA in a low-prevalence area. From 681 patients with a high risk for MRSA carriage, 1,601 specimens were collected and transported in Amies agar. After discordant analysis, the sensitivity, specificity, positive predictive value, and negative predictive value of the BD GeneOhm MRSA assay were 84.3%, 99.2%, 88.4%, and 98.9%, respectively, compared to culture.
Output: What is MRSA?
Neurofibromatosis type 1 (NF1) is an autosomal dominant genetic disorder affecting one in 3,500 individuals. The mutation rate in the NF1 gene is one of the highest known for human genes. Compared to other methods, the protein truncation test (PTT) provides improved efficiency in detecting NF1 mutations which are dispersed throughout the gene which spans 350 kilobases of genomic DNA. We have applied the PTT and subsequent sequence analysis of cloned cDNA to identify mutations in NF1 patients. We report here the identification of two novel (W336X and Q315X), and one recurrent (R304X) mutation located in exon 7 and show that all three premature termination codons lead to skipping of exon 7 in a proportion of the transcripts derived from the mutated allele. Possible mutation-induced alterations of the RNA secondary structure and their impact on skipping of exon 7 of the NF1 gene are explored and discussed.
Output: Which is the gene mutated in type 1 neurofibromatosis?
Do you know of anyone who uses the truth to deceive ? When someone tells you something that is true, but leaves out important information that should be included, he can give you a false picture. For example, some might say, "I just won a hundred dollars on the lottery . It was great. I took that dollar ticket back to the store and turned it in for one hundred dollars!" This guy's a winner, right? Maybe, maybe not. We then discover that he bought $200 worth of tickets, and only one was a winner. He's really a big loser! www. .com He didn't say anything that was false, but he left out important information on purpose. That's called a half-truth. Half-truths are not technically lies, but they are just as dishonest. Some politicians often use this trick. Let's say that during Governor Smith's last term, her state lost one million jobs and gained three million jobs. Then she seeked another term. One of her opponents said, "During Governor Smith's term, the state lost one million jobs!" That's true. However, an honest statement would have been, "During Governor Smith's term, the state had a net gain of two million jobs." Advertisers will sometimes use half-truths. It's against the law to make false statements so they try to mislead you with the truth. An advertisement might say, "Nine out of ten doctors advised their patients to take Yucky Pills to cure toothache." It fails to mention that they only asked ten doctors and nine of them work for the Yucky Company. This kind of deception happens too often. Lies are lies, and sometimes the truth can lie as well, which is a sad fact of life.
Output: How much did the lottery winner lose?
Restless legs syndrome (RLS), also known as Willis-Ekbom disease, is characterised by abnormal sensations in the legs as well as dysaesthesia. Although the aetiology of RLS has not yet been determined, it may be associated with systemic inflammation. The neutrophil-to-lymphocyte ratio (NLR) is a new and simple marker indicating systemic inflammation. The present study aimed to investigate the relationship between systemic inflammation and RLS through the use of the NLR. A total of 75 newly diagnosed patients with RLS and 56 healthy control subjects were included in the study. Baseline NLR was calculated by dividing the absolute neutrophil count by the absolute lymphocyte count. The NLRs of the two groups were compared. There were no significant differences in gender and age between the two groups. The NLR was 1.96 0.66 in the patient group and 1.67 0.68 in the control group (p = 0.005). Receiver operating characteristic analysis was performed to determine the cut-off value of NLR to predict RLS. The NLR was predictive at 1.58 with a 64% sensitivity and 50% specificity (95% confidence interval 0.55-0.74, area under curve 0.648 0.05). The NLR was found to be statistically higher in patients with RLS and may be used to predict RLS. The aetiology of RLS remains undetermined. The present study showed that systemic inflammation may play a role in RLS. However, RLS could also be associated with systemic inflammatory diseases. This relationship is supported by high NLR values, which are related to chronic systemic inflammation.
Output: Willis-Ekbom disease is also known as?
The Ruling Voice is a 1931 American Pre Code gangster drama directed by Rowland V. Lee, starring Walter Huston, Loretta Young and Doris Kenyon.
Output: In which The Ruling Voice a 1909 american silent drama film directed by?
Mowat-Wilson syndrome (MWS) is a severe intellectual disability (ID)-distinctive facial gestalt-multiple congenital anomaly syndrome, commonly associating microcephaly, epilepsy, corpus callosum agenesis, conotruncal heart defects, urogenital malformations and Hirschsprung disease (HSCR). MWS is caused by de novo heterozygous mutations in the ZEB2 gene. The majority of mutations lead to haplo-insufficiency through premature stop codons or large gene deletions. Only three missense mutations have been reported so far; none of which resides in a known functional domain of ZEB2. In this study, we report and analyze the functional consequences of three novel missense mutations, p.Tyr1055Cys, p.Ser1071Pro and p.His1045Arg, identified in the highly conserved C-zinc-finger (C-ZF) domain of ZEB2. Patients' phenotype included the facial gestalt of MWS and moderate ID, but no microcephaly, heart defects or HSCR. In vitro studies showed that all the three mutations prevented binding and repression of the E-cadherin promoter, a characterized ZEB2 target gene. Taking advantage of the zebrafish morphant technology, we performed rescue experiments using wild-type (WT) and mutant human ZEB2 mRNAs. Variable, mutation-dependent, embryo rescue, correlating with the severity of patients' phenotype, was observed. Our data provide evidence that these missense mutations cause a partial loss of function of ZEB2, suggesting that its role is not restricted to repression of E-cadherin. Functional domains other than C-ZF may play a role in early embryonic development. Finally, these findings broaden the clinical spectrum of ZEB2 mutations, indicating that MWS ought to be considered in patients with lesser degrees of ID and a suggestive facial gestalt, even in the absence of congenital malformation.
Output: Which gene is responsible for the development of the Mowat-Wilson syndrome?
USS Benefit (1863) was a steamer acquired by the Union Navy during the American Civil War.
Output: What is the war during which USS Benefit (1863) was in the armed forces?
BugSat 1 was launched from Dombarovsky (air base) site 13, Russia, on 19 June 2014 by a Dnepr-1 rocket.
Output: What date was BugSat 1 launched?
Scientists in Mexico have just begun a new study of one of the world's biggest pyramids: the Pyramid of the Sun, north of Mexico City. They're putting lots of small, high-tech machines under the pyramid to try to unlock some of its secrets. For thousands of years, people have tried to uncover the secrets of the pyramids. The people who built the pyramids made lots of secret doors and rooms to stop robbers from finding the treasures inside. However, there always have been some smart thieves in history. Now, almost all of Egypt's pyramids have been robbed, including the Great Pyramid of Khufu, which is the largest pyramid in the world. It is the only one of the ancient wonders of the world that is still standing. In AD 820, an Arab king named Abdullah AL Manum got a group of workers to dig their way into the Great Pyramid and have a look. Inside the pyramid, they found three rooms --- the Queen's Chamber, the Grand Gallery and the King's Chamber. But to their surprise, the men didn't find the treasures they wanted. The Queen's and King's Chambers were both empty! Where were the King's mummy and his treasures? Had someone already taken them away? The huge stone doors at the pyramid's entrance were still closed when AL Manum's men went inside. How had the thieves got in and out? Since then, many people have gone inside the Great Pyramid to have a look or to try to take things. But still, no other chambers or walkways have been found. In 2002, an American team made the most recent visit to the Great Pyramid. Scientists sent a robot into the pyramid, but they only found a mysterious locked stone door.
Output: What is the purpose of making some secret doors to the pyramids?
Ghrelin exhibits its biological effect through binding to the growth hormone secretagogue 1a receptor (GHS-R1a). Recently, it has been reported that ghrelin has an anti-apoptotic effect in several cell types. However, the molecule mechanisms underlying the anti-apoptotic effect of ghrelin remain poorly understood. In this study, we investigated the intracellular mechanisms responsible for anti-apoptotic effect of ghrelin on human umbilical vein endothelial cells (HUVEC). Treatment of HUVEC with ghrelin inhibited high glucose-induced cell apoptosis. Ghrelin stimulated the rapid phosphorylation of mammalian target of rapamycin (mTOR), P70S6K and S6. The GHS-R1a-specific antagonist [D-Lys3]-GHRP-6 abolished the anti-apoptotic effect and inhibited the activation of mTOR, P70S6K, S6 induced by ghrelin. Pretreatment of cells with specific inhibitor of mTOR blocked the anti-apoptotic effect of ghrelin. In addition, ghrelin protected HUVECs against high glucose induced apoptosis by increasing Bcl-2/Bax ratio. Taken together, our results demonstrate that ghrelin produces a protective effect on HUVECs through activating GHS-R1a and mTOR/P70S6K signaling pathway mediates the effect of ghrelin. These observations suggest that ghrelin may act as a survival factor in preventing HUVECs apoptosis caused by high glucose.
Output: What does mTOR stands for?
What could make Michael Jordon, one of the greatest basketball players in history, shed tears? It was when he became a member of the Basketball Hall of Fame on September 11. The hall is in Springfield, Massachusetts and honors the best basketball players, greatest coaches and other contributors to the game. "The game of basketball has been everything to me ," said Jordan, 46. "It is the place I have always gone when I needed to find comfort and peace." Jordan retired twice in his 15-year career, finishing with 32292 points, the third highest total points in NBA history behind Kareem Abdul-Jabbar and Karl Malone. His average of 30.12 points a game is the best in the League. "One day you might look up and see me playing the game at 50," Jordan said. "Because limits, like fears, are often just an illusion." In other words, it was motivation that made Jordan such a success.. A great career did not mean a smooth one. His high school coach cut him from the team in favor of a taller player when Jordan was in 10thgrade. His college coach wouldn't allow him on the cover of Sports Illustration in 1981 with North Carolina's four other players because Jordan was a freshman. In his first NBA All-Star game, older NBA stars wouldn't throw him the ball because of jealousy. "That burned me up," Jordan said. "But _ don't have to stop you. If you run into a wall, don't turn around and give up. Figure out how to climb it, go through it, or work around it."
Output: Who scored the highest total points in NBA history?
There is considerable interest in developing new multipurpose prevention technologies to address women's reproductive health needs. This study describes an innovative barrier contraceptive device--based on the SILCS diaphragm--that also provides long-term controlled release of the lead candidate anti-HIV microbicide dapivirine. Diaphragm devices comprising various dapivirine-loaded polymer spring cores overmolded with a nonmedicated silicone elastomer sheath were fabricated by injection molding processes. In vitro release testing, thermal analysis and mechanical characterization were performed on the devices. A diaphragm device containing a polyoxymethylene spring core loaded with 10% w/w dapivirine provided continuous and controlled release of dapivirine over a 6-month period, with a mean in vitro daily release rate of 174 mcg/day. The mechanical properties of the new diaphragm were closely matched to the SILCS diaphragm. The study demonstrates proof of concept for a dapivirine-releasing diaphragm with daily release quantities potentially capable of preventing HIV transmission. In discontinuous clinical use, release of dapivirine may be readily extended over 1 or more years.
Output: Which infection can be prevented with Dapivirine?
In the Caucasus region of Russia, nearly 50 out of every 100,000 people live to celebrate their 100th birthday, and many don't stop at 100! By comparison, in America only 3 people in 100,000 reach 100. But these Russian old people aren't alone. The Pakistanis, who live high in the Himalaya Mountains, and the Ecuadorans of the Andes Mountains seem to share the secret of long life, too. These people remain healthy in body and spirit despite the passage of time. While many older persons in industrial societies become weak and ill in their 60s and 70s, some Caucasians aged 100 to 140, work in the fields beside their great-great-grandchildren. Even the idea of aging is foreign to them. When asked "at what age does youth end?"most of these old people had no answer. Several replied, "Well, perhaps at age 80." What accounts for this ability to survive to such old age, and to survive so well?First of all, hard physical work is a way of life for all of these long-lived people. They begin their long days of physical labor as children and never seem to stop. For example, Mr . Rustam Mamedov is 142 years of age. His wife is 116 years old. They have been married for 90 years. Mr. Mamedov has no intention of retiring from his life as a farmer. "Why?What else would I do?"he asks. All these people get healthful rewards from the environment in which they work. They all come from mountainous regions. They live and work at elevations of 1,660 to 1,000 meters above sea level. The air has less oxygen and is pollution-free. This reduced-oxygen environment makes the heart and blood vessel system stronger. Another factor that may contribute to the good health of these people is their isolation. To a great extent, they are separated from the pressures and worries of industrial society. Inherited factors also play some role. Most of the longest-lived people had parents and grandparents who also reached very old ages. Good family genes may, therefore, be one factor in living longer.
Output: What is the main way of life for all of these long-lived people?
In cancer research, background models for mutation rates have been extensively calibrated in coding regions, leading to the identification of many driver genes, recurrently mutated more than expected. Noncoding regions are also associated with disease; however, background models for them have not been investigated in as much detail. This is partially due to limited noncoding functional annotation. Also, great mutation heterogeneity and potential correlations between neighboring sites give rise to substantial overdispersion in mutation count, resulting in problematic background rate estimation. Here, we address these issues with a new computational framework called LARVA. It integrates variants with a comprehensive set of noncoding functional elements, modeling the mutation counts of the elements with a -binomial distribution to handle overdispersion. LARVA, moreover, uses regional genomic features such as replication timing to better estimate local mutation rates and mutational hotspots. We demonstrate LARVA's effectiveness on 760 whole-genome tumor sequences, showing that it identifies well-known noncoding drivers, such as mutations in the TERT promoter. Furthermore, LARVA highlights several novel highly mutated regulatory sites that could potentially be noncoding drivers. We make LARVA available as a software tool and release our highly mutated annotations as an online resource (larva.gersteinlab.org).
Output: Which tool is used for the identification of recurrent variants in noncoding regions?
A central feature of Alzheimer's disease is the cleavage of the amyloid precursor protein (APP) to form beta-amyloid peptide (Abeta) by the beta-secretase and gamma-secretase enzymes. Although this has been shown to occur after endocytosis of APP from the cell surface, the exact compartments of APP processing are not well defined. We have previously demonstrated that APP and gamma-secretase proteins and activity are highly enriched in purified rat liver lysosomes. In order to examine the lysosomal distribution and trafficking of APP in cultured cells, we generated constructs containing APP fused to a C-terminal fluorescent protein tag and N-terminal HA-epitope tag. These were co-transfected with a panel of fluorescent-protein tagged compartment markers. Here we demonstrate using laser-scanning confocal microscopy that although APP is present throughout the endosomal/lysosomal system in transfected Cos7 and neuronal SN56 cell lines as well as in immunostained cultured mouse neurons, it is enriched in the lysosome. We also show that the Swedish and London mutations reduce the amount of APP in the lysosome. Surprisingly, in addition to its expected trafficking from the cell surface to the early and then late endosomes, we find that cell-surface labelled APP is transported rapidly and directly from the cell surface to lysosomes in both Cos7 and SN56 cells. This rapid transit to the lysosome is blocked by the presence of either the London or Swedish mutations. These results demonstrate the presence of a novel, rapid and specific transport pathway from the cell surface to the lysosomes. This suggests that regulation of lysosomal traffic could regulate APP processing and that the lysosome could play a central role in the pathophysiology of Alzheimer's disease.
Output: Which disease the London mutation involved in?