niryuu/sni-each-converted huggingface dataset:
The CRISPR-Cas (clustered regularly interspaced short palindromic repeats, CRISPR-associated genes) is an adaptive immunity system in bacteria and archaea that functions via a distinct self-non-self recognition mechanism that is partially analogous to the mechanism of eukaryotic RNA interference (RNAi). The CRISPR-Cas system incorporates fragments of virus or plasmid DNA into the CRISPR repeat cassettes and employs the processed transcripts of these spacers as guide RNAs to cleave the cognate foreign DNA or RNA. The Cas proteins, however, are not homologous to the proteins involved in RNAi and comprise numerous, highly diverged families. The majority of the Cas proteins contain diverse variants of the RNA recognition motif (RRM), a widespread RNA-binding domain. Despite the fast evolution that is typical of the cas genes, the presence of diverse versions of the RRM in most Cas proteins provides for a simple scenario for the evolution of the three distinct types of CRISPR-cas systems. In addition to several proteins that are directly implicated in the immune response, the cas genes encode a variety of proteins that are homologous to prokaryotic toxins that typically possess nuclease activity. The predicted toxins associated with CRISPR-Cas systems include the essential Cas2 protein, proteins of COG1517 that, in addition to a ligand-binding domain and a helix-turn-helix domain, typically contain different nuclease domains and several other predicted nucleases. The tight association of the CRISPR-Cas immunity systems with predicted toxins that, upon activation, would induce dormancy or cell death suggests that adaptive immunity and dormancy/suicide response are functionally coupled. Such coupling could manifest in the persistence state being induced and potentially providing conditions for more effective action of the immune system or in cell death being triggered when immunity fails.
Output: Gene silencing can be achieved by RNA interference (RNAi) in eukaryotic organisms. What is the name of the analogous process in prokaryotic organisms?
Munich sincerely invites you to join the 2016 0ktoberfest--the biggest celebration of its kind in Europe. Drink beer by the liter, eat traditional Bavarian food, listen to traditional Bavarian music as well as more up-to-date music, and enjoy yourself with hundreds of other people from all over the world, dancing and singing the hours away. Oktoberfest 2016 begins on Saturday 17th September and runs through to Monday 3rd October 2016. Don't miss the events during Oktoberfest! Grand entry of the Oktoberfest landlords and breweries Date: Saturday, 17th September 2016 Duration: about 45 minutes This is the official prelude to the opening of the Oktoberfest and involves about 1,000 participants, including the landlords' families in decorated carriages, the magnificent horse-drawn drays, waitresses on decorated floats and all the beer tent bands. Oktoberfest Costume and Riflemen-s Parade Date: Sunday, 18th September 2016 Duration:2-2 1/2 hours, about 8000 performers,7 km distance A varied succession of regional costume groups, "troops" in historical uniforms, marching bands, riflemen, thoroughbred horses, oxen, cows, goats, floats displaying typical local traditions and historic carriages all pass by in a 7-kilometer-long procession through the streets of the city center. Open-Air Oktoberfest Music Date: Sunday, 25th September 2016 Place: on the steps of the Bavaria statue At the traditional open-air concert on the second Sunday of the Oktoberfest, all the instruments that make up typical Bavarian wind band music can be heard. The bands from all of the tents come together at the steps under the Bavaria statue to play the most well-known songs and marches of Oktoberfest together while being conducted by distinguished Munich personalities.
Output: When will visitors see costume soldiers marching on the street?
Chikondi Banda (28 December 1979 -- 8 August 2013) was a Malawian international footballer who played as a midfielder.
Output: In which position does Chikondi Banda play?
The organization of chromatin into higher-order structures influences chromosome function and epigenetic gene regulation. Higher-order chromatin has been proposed to be nucleated by the covalent modification of histone tails and the subsequent establishment of chromosomal subdomains by non-histone modifier factors. Here we show that human SUV39H1 and murine Suv39h1--mammalian homologues of Drosophila Su(var)3-9 and of Schizosaccharomyces pombe clr4--encode histone H3-specific methyltransferases that selectively methylate lysine 9 of the amino terminus of histone H3 in vitro. We mapped the catalytic motif to the evolutionarily conserved SET domain, which requires adjacent cysteine-rich regions to confer histone methyltransferase activity. Methylation of lysine 9 interferes with phosphorylation of serine 10, but is also influenced by pre-existing modifications in the amino terminus of H3. In vivo, deregulated SUV39H1 or disrupted Suv39h activity modulate H3 serine 10 phosphorylation in native chromatin and induce aberrant mitotic divisions. Our data reveal a functional interdependence of site-specific H3 tail modifications and suggest a dynamic mechanism for the regulation of higher-order chromatin.
Output: What is the characteristic domain of histone methyltransferases?
Shurooq Amin was born in Kuwait in 1967 to a Kuwaiti father and a Syrian mother.
Output: What is the place of birth of Shurooq Amin?
Bokermannohyla is a genus of frogs in the Hylidae family.
Output: Is Bokermannohyla a genus or species?
The James Bay mine is one of the largest lithium mines in Canada.
Output: What product does James Bay mine make?
Schimke immuno-osseous dysplasia (OMIM 242900) is an uncommon autosomal-recessive multisystem disease caused by mutations in SMARCAL1 (swi/snf-related, matrix-associated, actin-dependent regulator of chromatin, subfamily a-like 1), a gene encoding a putative chromatin remodeling protein. Neurologic manifestations identified to date relate to enhanced atherosclerosis and cerebrovascular disease. Based on a clinical survey, we determined that half of Schimke immuno-osseous dysplasia patients have a small head circumference, and 15% have social, language, motor, or cognitive abnormalities. Postmortem examination of 2 Schimke immuno-osseous dysplasia patients showed low brain weights and subtle brain histologic abnormalities suggestive of perturbed neuron-glial migration such as heterotopia, irregular cortical thickness, incomplete gyral formation, and poor definition of cortical layers. We found that SMARCAL1 is highly expressed in the developing and adult mouse and human brain, including neural precursors and neuronal lineage cells. These observations suggest that SMARCAL1 deficiency may influence brain development and function in addition to its previously recognized effect on cerebral circulation.
Output: Mutations in which gene cause Schimke immune-osseous dysplasia?
Recombinant tissue plasminogen activator (rT-PA) is an effective treatment for acute ischaemic stroke when given within 3h of symptom onset but can be delayed as patients wait for a diagnosis. The ROSIER assessment tool (Fig. 1) has been found to be effective in diagnosing stroke but to date has only been tested when used by doctors. To compare registered nurses' ability to diagnose stroke using the ROSIER assessment tool with doctors' ability to diagnose stroke using traditional neurological assessment. A prospective audit of all suspected stroke patients (n=106) admitted to the stroke unit of a district general hospital over an eight month period, assessed by registered nurses trained to use the ROSIER assessment tool to identify stroke. Time from admission to the stroke unit until initial assessment by doctors and registered nurses. Comparison of initial diagnosis by doctors and registered nurses with final diagnosis by a consultant for stroke. Of 106 suspected stroke patients, 78 (73.5%) had a final diagnosis of stroke or transient ischaemic attack (TIA) and 28 (26.4%) had an alternative diagnosis. Six patients with TIA were subsequently excluded as they were asymptomatic at the time of assessment, leaving 100 participants in the validation phase of the study. Using the ROSIER tool registered nurses achieved a diagnostic sensitivity for stroke of 98% (95% confidence interval 88-99), positive predictive value (PPV) 83% (95% confidence interval 73-90). Doctors using standard neurological assessment had a similar diagnostic sensitivity of 94% (95% confidence interval 86-98), PPV 80% (95% confidence interval 70-88). The mean time from initial assessment by registered nurses using the ROSIER tool, until assessment by doctor on the stroke unit was 75 min (SD=65.8 min). Registered nurses working on a stroke unit using the ROSIER assessment tool are able to diagnose stroke with a degree of accuracy comparable to doctors using clinical neurological assessment. Prompt assessment of suspected stroke patients by registered nurses using the ROSIER tool could reduce delays in eligible stroke patients being assessed for rT-PA treatment.
Output: ROSIER scale is used for which disorder?
Alexey Suetin was married to Woman Grandmaster Kira Zvorykina and together they had a son Aleksandr, born 1951.
Output: What is Alexey Suetin's spouse's name?
Potone (Greek: Potone or Potone; born before 427 BC) daughter of Ariston and Perictione, was Plato's older sister.
Output: Who is the mother of Potone?
Exosomes are a subset of extracellular vesicles (EVs) that have important roles in intercellular communication. They contain and carry bioactive molecules within their membranes which are delivered to target cells. Reproducible isolation and enrichment of these exosomes will aid in evaluation of cellular communication. We present an approach that involved the pre-processing of plasma, combined with ultracentrifugation (UC) and size exclusion chromatography (SEC) to isolate EVs and subsequently enrich exosomes. Four variations of this approach (denoted methods I to IV) were compared. Coupling an ultracentrifugation method with size exclusion chromatography (Method II) provided the best yield by nanoparticle tracking analyses (NTA), the presence of the exosomal markers CD63, Flotillin-1 and TSG-101 (immunoblotting) and showed exosome morphology using transmission electron microscopy (TEM). This method provides an efficient way to enrich the exosomes from blood (plasma), which could be potentially employed for clinical diagnostic assessment and therapeutic intervention.
Output: What is an exosome?
Bernard de Castanet (c. 1240 -- 14 August 1317) was the bishop and cardinal of the Roman Catholic Church.
Output: Which was the position that Bernard de Castanet held?
5805 Glasgow is a main-belt asteroid which was discovered by Edward L. G. Bowell at the Anderson Mesa Station of the Lowell Observatory in Flagstaff, Arizona, on 18 December 1985.
Output: The 5805 Glasgow was discovered by whom?
A mother of a cheerful five-year-old just returned from a meeting of the National Organization for Women. Inspired by exciting dreams for the possibilities of womanhood, she asked her daughter what she wanted to be when she grew up. Little Lisa quickly answered, "A nurse." There was a time when nursing was thought of as a woman's job and the answer somehow seemed not to be satisfied. She had, after all, just returned from a new meeting. "You can be anything you want to be," she reminded her daughter. "You can be a lawyer, a doctor, a banker, president of the country -- you can be anything." "Anything?" Lisa asked. "Anything!" her mother smiled. "I know," Lisa said. "I want to be a horse!" Lisa's dream may need some improvement, but there is plenty of time for that. When do we give up dreaming about the future? When do we resign ourselves to simply replaying dreams from the past? Maybe her dream needs to mature a bit, but would you rather have the optimism of a five-year-old girl who wants to be a horse, or the pessimism of an adult who says in despair, "I can't be anything at all"? Teddy Roosevelt said, _ believe that is the way to make those dreams come true. It begins with looking up and dreaming of something beautiful.
Output: What did the girl really want to be when she grew up?
Since the early 2000s, the incidence of methicillin-resistant Staphylococcus aureus (MRSA) infections among the community of people lacking known healthcare risk factors has increased. This MRSA infection is referred to as community-associated MRSA (CA-MRSA) infection and is distinct from hospital-associated MRSA (HA-MRSA) infection, which occurs among people with known healthcare risk factors. Understanding the epidemiology of CA-MRSA infections is critical; however, this has not been investigated in detail in Japan. Our objective was to investigate the incidence of CA-MRSA infections in a regional hospital. We investigated CA-MRSA isolates and infections in a rural regional hospital by reviewing medical records of one year. Infections were classified as CA-MRSA if no established risk factors were identified. During 2008, 31 Staphylococcus aureus (S. aureus) isolates were detected in 29 unique patients, with 1 methicillin-sensitive S. aureus (MSSA) isolates obtained from 19 patients (66%) and MRSA obtained from 10 patients (34%). In the 10 patients with MRSA, the number of HA-MRSA and CA-MRSA cases were nine (32% of patients with S. aureus isolates) and one (3%), respectively. The patient with CA-MRSA was diagnosed with cellulitis due to CA-MRSA. All nine patients with HA-MRSA exhibited colonization. We observed a CA-MRSA case in a regional hospital in Japan, suggesting that incidence trends of CA-MRSA should be considered in future research and treatment.
Output: What is MRSA?
The human fibulin-1 gene, FBLN1, encodes four splice variants designated fibulin-1A, B, C and D, which differ in their carboxy terminal regions.
Output: In what living being can FBLN1 be found?
Fredrikke Marie Qvam (31 May 1843 -- 10 September 1938) was a Norwegian humanitarian leader, feminist and the wife of Prime Minister Ole Anton Qvam.
Output: What is Fredrikke Marie Qvam's spouse's name?
Rigel Pharmaceuticals Inc is developing fostamatinib, a prodrug of the spleen tyrosine kinase (Syk) inhibitor R-406, for the potential treatment of autoimmune diseases such as rheumatoid arthritis (RA), idiopathic thrombocytopenic purpura (ITP) and B-cell lymphomas. Syk is a key mediator of Fc and B-cell receptor signaling in inflammatory cells, such as B-cells, mast cells, macrophages and neutrophils. Preclinical studies of R-406 or fostamatinib demonstrated a significant reduction in major inflammatory mediators such as TNFalpha, IL-1, IL-6 and IL-18, leading to reduced inflammation and bone degradation in models of RA. In a phase II clinical trial, fostamatinib treatment effectively improved American College of Rheumatology response rates in patients with RA. Preclinical studies and phase II trials also suggested the potential of using fostamatinib for the treatment of ITP and B-cell lymphomas, by increasing platelet counts and inducing response rates, respectively. Fostamatinib is orally bioavailable and was well tolerated in phase I and II trials, with the most common side effect being gastrointestinal symptoms. At the time of publication, phase II trials for fostamatinib were ongoing in patients with RA, ITP and B-cell lymphomas. The Syk inhibitor appears to be a promising therapeutic for immunological diseases, but further data are required to establish the efficacy and long-term safety of the drug in humans.
Output: Which enzyme is inhibited by a drug fostamatinib?
Berts dagbok (Swedish: Bert's diary), translated as In Ned's Head, is a diary novel, written by Anders Jacobsson and Soren Olsson and originally published in 1987, it tells the story of Bert Ljung from 14 January to 4 June during the calendar year he turns 12 during the spring term in the 5th grade at school in Sweden.
Output: Who is the illustrator of Berts dagbok?
You have been badly injured in a car accident. It is necessary to give you a blood transfusion because you lost a great deal of blood in the accident. However, special care must be taken in selecting new blood for you. If the blood is too different from your own, the transfusion could kill you. There are four basic types of blood; A, B, AB, and O. A simple test can make sure of a person's blood type. Everybody is born with one of these four types of blood. Blood type, like hair color1 and height, is received from parents. The four groups must be transfused carefully. A and B cannot be mixed. A and B cannot receive AB, but AB may receive A or B. O can give to any other group; therefore, it is often called the universal donor . For the opposite reason, AB is sometimes called the universal recipient . However, because so many reactions can happen in transfusions , patients usually receive only salt of plasma (liquid part of blood) until their blood can be matched as exactly as possible in the blood bank of a hospital. In this way, it is possible to prevent the transfusion from any bad reactions.
Output: People with type A blood can receive type     .
Aurum Hotels was an Italian professional cycling team founded in 1996 and disbanded in 2007.
Output: What year did Aurum Hotels dissolve?
Kimball Cho is a fictional character portrayed by Tim Kang on the American TV crime drama The Mentalist.
Output: To which fictional work does Kimball Cho appear in?
Opioid overdose is rarely the primary cause of altered mental status in cancer patients receiving opioid therapy. The inappropriate administration of naloxone to reverse an abnormal mental status can cause severe withdrawal symptoms and pain. To illustrate this problem, we report the case of a patient inappropriately treated with naloxone and the results of a retrospective review of the medical records of 15 consecutive patients with cancer treated with naloxone in the emergency department over a 5-month period. We offer guidelines for a more thoughtful approach to the management of patients with cancer who present with encephalopathy.
Output: Which medication should be administered when managing patients with suspected acute opioid overdose?
The Page Ministry was the twenty-fifth Australian Commonwealth ministry, and ran from 7 April 1939 to 26 April 1939.
Output: What year did Page Ministry start?
The Banquet of the Officers of the St George Militia Company in 1627 refers to a schutterstuk painted by Frans Hals for the St. George (or St. Joris) civic guard of Haarlem, and today is considered one of the main attractions of the Frans Hals Museum there.
Output: What is the name of the place where The Banquet of the Officers of the St George Militia Company in 1627 can be found?
The Ehlers-Danlos Syndrome (EDS) is a rare connective tissue disorder characterised by fragility of the soft connective tissues and widespread manifestations in skin, ligaments, joints, blood vessels and internal organs. We report a case of a 12-year-old boy, previously diagnosed with kyphoscoliosis-type EDS (type VI), presenting with a left brachial artery pseudo-aneursym with history of multiple spontaneous and post-traumatic arterial ruptures. Surgical management of this patient was performed successfully by primary repair of brachial artery lesion.
Output: What tissue is most affected in Ehlers-Danlos syndromes?
Centrosomes are composed of a centriole pair surrounded by an intricate proteinaceous matrix referred to as pericentriolar material. Although the mechanisms underpinning the control of centriole duplication are now well understood, we know relatively little about the control of centrosome size and shape. Here we used interaction proteomics to identify the E3 ligase HERC2 and the neuralized homologue NEURL4 as novel interaction partners of the centrosomal protein CP110. Using high resolution imaging, we find that HERC2 and NEURL4 localize to the centrosome and that interfering with their function alters centrosome morphology through the appearance of aberrant filamentous structures that stain for a subset of pericentriolar material proteins including pericentrin and CEP135. Using an RNA interference-resistant transgene approach in combination with structure-function analyses, we show that the association between CP110 and HERC2 depends on nonoverlapping regions of NEURL4. Whereas CP110 binding to NEURL4 is dispensable for the regulation of pericentriolar material architecture, its association with HERC2 is required to maintain normal centrosome integrity. NEURL4 is a substrate of HERC2, and together these results indicate that the NEURL4-HERC2 complex participates in the ubiquitin-dependent regulation of centrosome architecture.
Output: Where in the cell do we find the protein Cep135?
Boy with Thorn, also called Fedele (Fedelino) or Spinario, is a Greco-Roman Hellenistic bronze sculpture of a boy withdrawing a thorn from the sole of his foot, now in the Palazzo dei Conservatori, Rome.
Output: What material was used for Boy with Thorn?
After independence, on 15 August 1947, Jawaharlal Nehru assumed office as the first Prime Minister of India and chose fifteen ministers to form the First Nehru ministry.
Output: Which person was the head of government of First Nehru ministry?
Although peak finding in next-generation sequencing (NGS) datasets has been addressed extensively, there is no consensus on how to analyze and process biological replicates. Furthermore, most peak finders do not focus on accurate determination of enrichment site widths and are not widely applicable to different types of datasets. We developed JAMM (Joint Analysis of NGS replicates via Mixture Model clustering): a peak finder that can integrate information from biological replicates, determine enrichment site widths accurately and resolve neighboring narrow peaks. JAMM is a universal peak finder that is applicable to different types of datasets. We show that JAMM is among the best performing peak finders in terms of site detection accuracy and in terms of accurate determination of enrichment sites widths. In addition, JAMM's replicate integration improves peak spatial resolution, sorting and peak finding accuracy. JAMM is available for free and can run on Linux machines through the command line: [Link]
Output: Which peak calling algorithm employs mixture model clustering under the hood?
In ''The Devil in Iron,'' an ancient demon, Khosatral Khel, is awakened on the remote island of Xapur due to the meddling of a greedy fisherman.
Output: Which place does The Devil in Iron exist in?
From 1948 to 1954, Ed Mikan played in the National Basketball Association as a member of the Chicago Stags, Rochester Royals, Washington Capitols, Philadelphia Warriors, Indianapolis Olympians, and Boston Celtics.
Output: Player Ed Mikan played for which team?
A total of about 790,000 tickets were put on sale from February 2015 online, at the Singapore Indoor Stadium and at SingPost outlets. To encourage public participation in the games, it was announced on 28 January 2015 that 18 of the sports will be free for spectators, while the other 18 are kept at relatively affordable levels of between S$5 to S$20. By 15 April 2015 some sports such as swimming and fencing were selling fast, while silat and sepak takraw were much less popular. Organisers reported that ticket sales were pass 70% for most sports by 27 April, with fencing and swimming being the most popular at 85 and 70% tickets sold respectively, while rhythmic gymnastics, silat and wushu have also sold over 70%. Billiards and snooker, equestrian, sepak takraw, taekwondo, and artistic gymnastics have sold about half their tickets. Rugby and judo have sold 30%, while table tennis has sold only 20%. Priced between S$5 to S$20, priority sales for the opening and closing ceremonies tickets for those who registered theirs interests in 2014 began on 15 January 2015, while sales for the general public began on 22 January 2015. In February 2015, tickets to the opening ceremony were going at over seven times their original value on unauthorised websites, despite 40% of the tickets still available at that time. By March 2015, tickets to the opening ceremony were sold out.
Output: What sport had sold the least by April 27?
Heterosexual transmission of human immunodeficiency virus (HIV) remains the major route of infection worldwide; thus, there is an urgent need for additional prevention strategies, particularly strategies that could be controlled by women, such as topical microbicides. Potential microbicide candidates must be both safe and effective. Using cellular and tissue explant models, we have evaluated the activity of the nonnucleoside reverse transcriptase inhibitor (NNRTI) dapivirine as a vaginal microbicide. In tissue compatibility studies, dapivirine was well tolerated by epithelial cells, T cells, macrophages, and cervical tissue explants. Dapivirine demonstrated potent dose-dependent inhibitory effects against a broad panel of HIV type 1 isolates from different clades. Furthermore, dapivirine demonstrated potent activity against a wide range of NNRTI-resistant isolates. In human cervical explant cultures, dapivirine was able not only to inhibit direct infection of mucosal tissue but also to prevent the dissemination of the virus by migratory cells. Activity was retained in the presence of semen or a cervical mucus simulant. Furthermore, dapivirine demonstrated prolonged inhibitory effects: it was able to prevent both localized and disseminated infection for as long as 6 days posttreatment. The prolonged protection observed following pretreatment of genital tissue and the lack of observable toxicity suggest that dapivirine has considerable promise as a potential microbicide candidate.
Output: Which infection can be prevented with Dapivirine?
Acanthomintha ilicifolia, known by the common name San Diego thornmint, is a rare species of flowering plant in the mint family.
Output: Is the taxon rank of Acanthomintha ilicifolia species or genus?
Jennifer Hamson (born January 23, 1992) is a professional basketball player who plays for the Los Angeles Sparks in the WNBA.
Output: What team does Jennifer Hamson belong to?
The product of the multiple endocrine neoplasia type 1 (MEN1) tumor suppressor gene, menin, is an integral component of MLL1/MLL2 histone methyltransferase complexes specific for Lys4 of histone H3 (H3K4). We show that menin is a transcriptional coactivator of the nuclear receptors for estrogen and vitamin D. Activation of the endogenous estrogen-responsive TFF1 (pS2) gene results in promoter recruitment of menin and in elevated trimethylation of H3K4. Knockdown of menin reduces both activated TFF1 (pS2) transcription and H3K4 trimethylation. In addition, menin can directly interact with the estrogen receptor-alpha (ERalpha) in a hormone-dependent manner. The majority of disease-related MEN1 mutations prevent menin-ERalpha interaction. Importantly, ERalpha-interacting mutants are also defective in coactivator function. Our results indicate that menin is a critical link between recruitment of histone methyltransferase complexes and nuclear receptor-mediated transcription.
Output: Which is the histone residue methylated by MLL1?
Nutrients in the foods you eat are needed by the cells of your body. How do the nutrients in foods get to your body cells? What organs and processes break down the foods and make the nutrients available to cells? The organs are those of the digestive system. The processes are digestion and absorption. The digestive system is the body system that breaks down food and absorbs nutrients. It also gets rid of solid food waste. The digestive system is mainly one long tube from the mouth to the anus, known as the gastrointestinal tract (GI tract). The main organs of the digestive system include the esophagus, stomach and the intestine, and are pictured below ( Figure 1.1). The intestine is divided into the small and large intestine. The small intestine has three segments. The ileum is the longest segment of the small intestine, which is well over 10 feet long. The large intestine is about 5 feet long. This drawing shows the major organs of the digestive system. The liver, pancreas and gallbladder are also organs of the digestive system. Digestion is the process of breaking down food into nutrients. There are two types of digestion, mechanical and chemical. In mechanical digestion, large chunks of food are broken down into small pieces. Mechanical digestion begins in the mouth and involves physical processes, such as chewing. This process continues in the stomach as the food is mixed with digestive juices. In chemical digestion, large food molecules are broken down into small nutrient molecules. This is a chemical process which also begins in the mouth as saliva begins to break down food and continues in the stomach as stomach enzymes further digest the food. Absorption is the process that allows substances you eat to be taken up by the blood. After food is broken down into small nutrient molecules, the molecules are absorbed by the blood. After absorption, the nutrient molecules travel in the bloodstream to cells throughout the body. This happens mostly in the small intestine. Some substances in food cannot be broken down into nutrients. They remain behind in the digestive system after the nutrients are absorbed. Any substances in food that cannot be digested and absorbed pass out of the body as solid waste. The process of passing solid food waste out of the body is called elimination.
Output: the mixing of food with saliva is the beginning of what process?
Carbide.c++ is a software development tool for C++ development on Symbian OS. It is used to develop phones that use the OS, as well as applications that run on those phones.
Output: What is the programming language for Carbide.c++?
The Third Bruce Ministry was the nineteenth Australian Commonwealth ministry, and ran from 29 November 1928 to 22 October 1929.
Output: What year did Third Bruce Ministry start?
Sodium-glucose cotransporter 2 (SGLT2) inhibitors lower glycemia by enhancing urinary glucose excretion. The physiologic response to pharmacologically induced acute or chronic glycosuria has not been investigated in human diabetes. We evaluated 66 patients with type 2 diabetes (62 7 years, BMI = 31.6 4.6 kg/m(2), HbA1c = 55 8 mmol/mol, mean SD) at baseline, after a single dose, and following 4-week treatment with empagliflozin (25 mg). At each time point, patients received a mixed meal coupled with dual-tracer glucose administration and indirect calorimetry. Both single-dose and chronic empagliflozin treatment caused glycosuria during fasting (median, 7.8 [interquartile range {IQR}, 4.4] g/3 hours and 9.2 [IQR, 5.2] g/3 hours) and after meal ingestion (median, 29.0 [IQR, 12.5] g/5 hours and 28.2 [IQR, 15.4] g/5 hours). After 3 hours of fasting, endogenous glucose production (EGP) was increased 25%, while glycemia was 0.9 0.7 mmol/l lower (P < 0.0001 vs. baseline). After meal ingestion, glucose and insulin AUC decreased, whereas the glucagon response increased (all P < 0.001). While oral glucose appearance was unchanged, EGP was increased (median, 40 [IQR, 14] g and 37 [IQR, 11] g vs. 34 [IQR, 11] g, both P < 0.01). Tissue glucose disposal was reduced (median, 75 [IQR, 16] g and 70 [IQR, 21] g vs. 93 [IQR, 18] g, P < 0.0001), due to a decrease in both glucose oxidation and nonoxidative glucose disposal, with a concomitant rise in lipid oxidation after chronic administration (all P < 0.01). Cell glucose sensitivity increased (median, 55 [IQR, 35] pmol min(-1) m(-2) mM(-1) and 55 [IQR, 39] pmol min(-1) m(-2) mM(-1) vs. 44 [IQR, 32] pmol min(-1) m(-2) mM(-1), P < 0.0001), and insulin sensitivity was improved. Resting energy expenditure rates and those after meal ingestion were unchanged. In patients with type 2 diabetes, empagliflozin-induced glycosuria improved cell function and insulin sensitivity, despite the fall in insulin secretion and tissue glucose disposal and the rise in EGP after one dose, thereby lowering fasting and postprandial glycemia. Chronic dosing shifted substrate utilization from carbohydrate to lipid. Trial registration. ClinicalTrials.Gov NCT01248364 (EudraCT no. 2010-018708-99). Funding. This study was funded by Boehringer Ingelheim.
Output: Which protein does empagliflozin inhibit?
Throughout 1476, supporters of Joanna from the nobility continued to submit to Isabella and Ferdinand, particularly those from the Pacheco-Giron lineage: Juan Tellez-Giron and his brother Rodrigo; Luis de Portocarrero; and, in September, the Marquis of Villena. In November 1476, Isabella's troops captured the castle of Toro. In the following months, they took control of the last bordering localities controlled by the Portuguese and dealt with their adversaries in Extremadura. In July 1477, Isabella arrived in Seville, the most populous city of Castile, with the objective of asserting her power over the nobility of Andalusia. In April 1476, Isabella and Ferdinand gave their first exculpation to the Marquis of Cadiz. He had been regaining power while his rival, the Duke of Medina Sidonia, initially the main Isabella supporter in Andalusia, had been falling into dishonour. Through skilful negotiations, the Queen managed to take control of the main strongholds of Seville occupied by the Marquis and the Duke and, instead of returning them to their legitimate owners, named others as their heads. She prohibited both nobles from entering the city of Seville, under the pretext that their simultaneous presence there would risk violent conflicts. In this way the Duke's political dominance over Seville disappeared, and the city passed into the control of the Crown. One of the few nobles that refused to submit to the monarchs was Marshall Fernan Arias de Saavedra. Isabella's troops laid siege to his fortress at Utrera, and conquered it by assault in March 1478. The defeated suffered harsh repression. The first son of the monarchs, John of Aragon and Castile, was born in Seville on June 30, 1478, which opened new possibilities for dynastic stability of the Isabellian side.
Output: What event happened first, Isabella arriving in Seville or Isabella and Ferdinand giving their first exculpation to the Marquis of Cadiz?
Genevieve Blatt (1913--1996) was an American politician and attorney from Pennsylvania, and a member of the Democratic Party.
Output: Which political party was Genevieve Blatt a member of?
The Eyes of Horror is an EP by American death metal band Possessed.
Output: Which artist or group performed The Eyes of Horror?
The skeletal and immune systems have a complex relationship. Both systems are intimately coupled, with osteoclastogenesis and hematopoiesis occurring in the bone marrow. Bone and immune cells also share common hematopoietic precursors. Furthermore, the skeletal and immune systems share various cytokines, receptors, and transcription factors that regulate signal transduction pathways involved in osteoclastogenesis and immune system activation, including the receptor activator of nuclear factor- ligand/receptor activator of nuclear factor-/osteoprotegerin (RANKL-RANK-OPG) pathway. Cancer cells can disrupt both the skeletal and immune systems. Interaction between cancer and bone cells results in a vicious cycle of bone destruction and cancer growth. Bone remodeling generates a growth-factor-rich environment that attracts cancer cells and promotes their proliferation. In turn, cancer cells stimulate osteoclast formation and activity, resulting in additional bone resorption that further stimulates cancer cell growth. Currently available bone-targeted therapies may also modulate the immune system. Bisphosphonates such as zoledronic acid exert stimulating effects on the immune system, resulting in possible anticancer activity against malignant cells. Denosumab, an anti-RANKL monoclonal antibody with proven antiosteoclast activity, may suppress immune responses. This may result in the reported association with an increased risk of neoplasms, as well as serious skin and other infections as reported in some studies, mainly in the postmenopausal setting. When assessing bone-targeted therapies, it is important to consider the shared signaling pathways between bone and the immune system, as well as the clinical risk:benefit ratio.
Output: To the ligand of which receptors does Denosumab (Prolia) bind?
There are many Spanish gossip magazines being published around the world. If your Spanish is good enough, these magazines will offer a lively look into the private moments of the beautiful, rich and famous, and provide entertainment and at the same time, help you practice your Spanish. DiezMinutos: The magazine is a classic Spanish gossip feast with daily news based on many stories of love, separation, divorce and death. The two main parts are headlined "love"and "partners".They also have an online version of the magazine for serious gossip addicts. Hola: It is Spain's top weekly magazine and the leader of the gossip world. It contains many pictures and a round-up of well-known and less well-known nobles and people in show business. Apart from edited highlights from the present and past issues, there is a report of the week and photo of the week. There is also a French version calledOhLa! RevistaCUORE: As the third best-selling gossip magazine in Spain, it is mainly aimed at younger teenage readers who look not only for current celebrity gossip, but also for fashion and TV news. It uses a lot of oralterms. RevistaSEMANA: It is a Spanish magazine covering the latest news on the famous in Spain and Hollywood. It also offers its readers information on fashion, beauty, cooking and travel. RevistaLECTURAS: Practical, friendly and informal, this magazine is one of the most widely-read gossip magazines on the Spanish market today. Marujeo.com: It is a blog serving up a daily diet of national gossip news on Spanish and international celebrities and the celebrity world from a particular point of view. RevistaCARAS: It is a magazine published in various countries of Latin America. It is also exported to certain parts of the United States, bringing together strange and wonderful news from around the world and the famous Latin community.
Output: How many of the magazines mentioned in the text can be read on the Internet?
The methylation of lysine residues of histones plays a pivotal role in the regulation of chromatin structure and gene expression. Here, we report two crystal structures of SET7/9, a histone methyltransferase (HMTase) that transfers methyl groups to Lys4 of histone H3, in complex with S-adenosyl-L-methionine (AdoMet) determined at 1.7 and 2.3 A resolution. The structures reveal an active site consisting of: (i) a binding pocket between the SET domain and a c-SET helix where an AdoMet molecule in an unusual conformation binds; (ii) a narrow substrate-specific channel that only unmethylated lysine residues can access; and (iii) a catalytic tyrosine residue. The methyl group of AdoMet is directed to the narrow channel where a substrate lysine enters from the opposite side. We demonstrate that SET7/9 can transfer two but not three methyl groups to unmodified Lys4 of H3 without substrate dissociation. The unusual features of the SET domain-containing HMTase discriminate between the un- and methylated lysine substrate, and the methylation sites for the histone H3 tail.
Output: What is the characteristic domain of histone methyltransferases?
Nitric oxide (NO) is involved in many pathological conditions including neurodegenerative disorders. We have previously found that sodium nitroprusside (SNP), an NO donor, stimulates mitogen-activated protein kinases (MAPKs) such as extracellular signal-regulating kinase (ERK), c-jun N-terminal protein kinase (JNK) and p38 MAPK, leading to caspase-independent apoptosis in cultured astrocytes. In view of the previous observation that NO stimulates the activity of the Na(+)/Ca(2+) exchanger (NCX), this study examines the involvement of NCX in cytotoxicity. The specific NCX inhibitor SEA0400 blocked SNP-induced phosphorylation of ERK, JNK and p38 MAPK, and decrease in cell viability. SNP-induced phosphorylation of ERK, JNK and p38 MAPK was blocked by removal of external Ca(2+), and SNP treatment caused an increase in (45)Ca(2+) influx. This increase in (45)Ca(2+) influx was blocked by SEA0400, but not the Ca(2+) channel blocker nifedipine. In addition, SNP-induced (45)Ca(2+) influx and cytotoxicity were reduced in NCX1-deficient cells which were transfected with NCX1 siRNA. Inhibitors of intracellular Ca(2+)-dependent proteins such as calpain and calmodulin blocked SNP-induced ERK phosphorylation and decrease in cell viability. Furthermore, the guanylate cyclase inhibitor LY83583 and the cGMP-dependent protein kinase inhibitor KT5823 blocked SNP-induced cytotoxicity. These findings suggest that NCX-mediated Ca(2+) influx triggers SNP-induced apoptosis in astrocytes, which may be mediated by a cGMP-dependent pathway.
Output: The small molecule SEA0400 is an inhibitor of which ion antiporter/exchanger?
We have investigated the contribution of Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) and mitogen-activated protein kinase (MAP kinase) in norepinephrine (NE)-induced arachidonic acid (AA) release in rabbit aortic vascular smooth muscle cells (VSMC). NE enhanced release of AA via activation of cytosolic phospholipase A2 (cPLA2) but not secretory PLA2 in VSMC prelabeled with [3H]AA. NE (10 microM) enhanced CaM kinase II and MAP kinase activity. In cells transiently transfected with antisense oligonucleotides complementary to the translation initiation sites of CaM kinase II and MAP kinase, NE-induced AA release was inhibited by 100 and 35% respectively. Treatment of cells with PD-098059, a MAP kinase kinase inhibitor, or with MAP kinase antisense oligonucleotide reduced NE-induced activation of MAP kinase and cPLA2. NE-induced MAP kinase and cPLA2 activation was also inhibited in cells treated with a CaM kinase II inhibitor, KN-93, or with CaM kinase II antisense oligonucleotide. On the other hand, inhibition of MAP kinase kinase with PD-098059 or of MAP kinase with antisense oligonucleotides did not alter the NE-induced increase in CaM kinase II activity. Phosphorylation of MAP kinase and CaM kinase II by NE, studied by 32P incorporation and immune complex kinase assays, was inhibited by KN-93. Collectively, these data suggest that CaM kinase II can activate MAP kinase, which in turn activates cPLA2 to release AA for prostacyclin synthesis in the rabbit VSMC. This novel pathway for activation of MAP kinase by CaM kinase II appears to be mediated through stimulation of MAP kinase kinase. Activation of adrenergic receptors with NE in VSMC caused translocation of CaM kinase II, MAP kinase, and cPLA2 to the nuclear envelope only in the presence of extracellular Ca2+. Okadaic acid, which increased phosphorylation and activity, did not translocate these enzymes. Therefore, it appears that in rabbit VSMC, NE, by promoting extracellular Ca2+ influx, increases CaM kinase II activity, leading to activation of MAP kinase and cPLA2 and translocation to the nuclear envelope, resulting in release of AA from the nuclear envelope for prostacyclin synthesis.
Output: Which kinase is inhibited by the small molecule KN-93?
Villa Saraceno has been dated to the 1540s, which makes it one of Andrea Palladio's earlier works.
Output: Who created Villa Saraceno?
Walter Lynn (born Valdimir (sic) Royal Lynn; 1 October 1928 -- 6 June 2011) was a distinguished professor at Cornell University for most of his academic career.
Output: What is Walter Lynn's place of employment?
Three isoforms of human plasma apolipoprotein E (apoE) are ligands to lipoprotein receptors and influence in different manner the synthesis and catabolism of pro-atherogenic triglyceride-rich lipoproteins. Among three isoforms, the apoE4 isoform is associated with increased frequency of atherosclerosis and Alzheimer's disease (AD). The conformational transitions of beta-amyloid (Abeta) influenced by apoE and serum amyloid P (SAP) component are key events in AD development, the accumulation of intermediate diffusible and soluble oligomers of Abeta being of particular significance. SAP and apoE, in a different manner for the three isoforms, serve as "pathological" chaperones during the aggregation of Abeta considered as a conformation-prone process. In turn, apoE consisting of two domains self-associates in solution and intermediate structures differently populated for the three isoforms exist. The different structures of the three isoforms determine their different distribution among various plasma lipoproteins. The structural and metabolic consideration of the common apoE pathway(s) in two pathologies assumes four molecular targets for AD correction: (i) inhibition of the accumulation of diffusible soluble Abeta oligomers; (ii) inhibition of apoE synthesis and secretion by astrocytes, in particular, under lipid-lowering therapy; (iii) inhibition of the binding of apoE and/or SAP to Abeta; (iv) stimulation of the expression of cholesterol transporter ABCA1.
Output: Which ApoE isoform is associated with atherosclerosis and Alzheimer's disease?
Restless legs syndrome is a neurological disorder characterized by an urgency to move the legs during periods of rest. Data from a variety of sources provide a compelling argument that the amount of iron in the brain is lower in individuals with restless legs syndrome compared with neurologically normal individuals. Moreover, a significant percentage of patients with restless legs syndrome are responsive to intravenous iron therapy. The mechanism underlying the decreased iron concentrations in restless legs syndrome brains is unknown. We hypothesize that the source of the brain iron deficit is at the blood-brain interface. Thus we analysed the expression of iron management proteins in the epithelial cells of the choroid plexus and the brain microvasculature in post-mortem tissues. The choroid plexus, obtained at autopsy, from 18 neurologically normal controls and 14 individuals who had primary restless legs syndrome was subjected to histochemical staining for iron and immunostaining for iron management proteins. Iron and heavy chain ferritin staining was reduced in the epithelial cells of choroid plexus in restless legs syndrome. Divalent metal transporter, ferroportin, transferrin and its receptor were upregulated in the choroid plexus in restless legs syndrome. Microvessels were isolated from the motor cortex of 11 restless legs syndrome and 14 control brains obtained at autopsy and quantitative immunoblot analyses was performed. Expression of heavy chain ferritin, transferrin and its receptor in the microvessels from restless legs syndrome was significantly decreased compared with the controls but divalent metal protein 1, ferroportin, prohepcidin, mitochondrial ferritin and light-chain ferritin remained unchanged. The presence of an iron regulatory protein was demonstrated in the brain microvasculature and the activity of this protein is decreased in restless legs syndrome; a finding similar to our earlier report in neuromelanin cells from the substantia nigra of restless legs syndrome brains. This study reveals that there are alterations in the iron management protein profile in restless legs syndrome compared with controls at the site of blood-brain interface suggesting fundamental differences in brain iron acquisition in individuals with restless legs syndrome. Furthermore, the decrease in transferrin receptor expression in the microvasculature in the presence of relative brain iron deficiency reported in restless legs syndrome brains may underlie the problems associated with brain iron acquisition in restless legs syndrome. The consistent finding of loss of iron regulatory protein activity in restless legs syndrome brain tissue further implicates this protein as a factor in the underlying cause of the iron deficiency in the restless legs syndrome brain. The data herein provide evidence for regulation of iron uptake and storage within brain microvessels that challenge the existing paradigm that the blood-brain barrier is merely a transport system.
Output: Which deficiency is the cause of restless leg syndrome?
The Witte Huis (Dutch pronunciation: (t ys)) or White House is a building and National Heritage Site in Rotterdam, Netherlands, built in 1898 in the Art Nouveau style.
Output: The art style of Witte Huis is what?