niryuu/sni-each-converted huggingface dataset:
The dynamin-related protein Opa1 is localized to the mitochondrial intermembrane space, where it facilitates fusion between mitochondria. Apoptosis causes Opa1 release into the cytosol and causes mitochondria to fragment. Loss of mitochondrial membrane potential also causes mitochondrial fragmentation but not Opa1 release into the cytosol. Both conditions induce the proteolytic cleavage of Opa1, suggesting that mitochondrial fragmentation is triggered by Opa1 inactivation. The opposite effect was observed with knockdown of the mitochondrial intermembrane space protease Yme1. Knockdown of Yme1 prevents the constitutive cleavage of a subset of Opa1 splice variants but does not affect carbonyl cyanide m-chlorophenyl hydrazone or apoptosis-induced cleavage. Knockdown of Yme1 also increases mitochondrial connectivity, but this effect is independent of Opa1 because it also occurs in Opa1 knockdown cells. We conclude that Yme1 constitutively regulates a subset of Opa1 isoforms and an unknown mitochondrial morphology protein, whereas the loss of membrane potential induces the further proteolysis of Opa1.
Output: Which is the cellular localization of the protein Opa1?
The genetic information of the cell, or DNA, is stored in the nucleus. During mitosis, two nuclei (plural for nucleus) must form, so that one nucleus can be in each of the new cells after the cell divides. In order to create two genetically identical nuclei, DNA inside of the nucleus must be copied or replicated. This occurs during the S phase of the cell cycle. During mitosis, the copied DNA is divided into two complete sets, so that after cytokinesis, each cell has a complete set of genetic instructions. To begin mitosis, the DNA in the nucleus wraps around proteins to form chromosomes. Each organism has a unique number of chromosomes. In human cells, our DNA is divided up into 23 pairs of chromosomes. Replicated DNA forms a chromosome made from two identical sister chromatids, forming an "X" shaped molecule ( Figure 1.1). The two chromatids are held together on the chromosome by the centromere. The centromere is also where spindle fiber microtubules attach during mitosis. The spindles separate sister chromatids from each other. During mitosis, the two sister chromatids must be divided. This is a precise process that has four individual phases to it. After the sister chromatids separate, each separate chromatid is now known as a chromosome. Each resulting chromosome is made of DNA from just one chromatid. So, each chromosome after this separation is made of "1/2 of the X." Through this process, each daughter cell receives one copy of each chromosome. The four phases of mitosis are prophase, metaphase, anaphase and telophase ( Figure 1.2). 1. Prophase: The chromatin, which is unwound DNA, condenses forming chromosomes. The DNA becomes so tightly wound that you can see them under a microscope. The membrane around the nucleus, called the nuclear envelope, disappears. Spindles also form and attach to chromosomes to help them move. 2. Metaphase: The chromosomes line up in the center, or the equator, of the cell. The chromosomes line up in a row, one on top of the next. 3. Anaphase: The two sister chromatids of each chromosome separate as the spindles pull the chromatids apart, resulting in two sets of identical chromosomes. 4. Telophase: The spindle dissolves and nuclear envelopes form around the chromosomes in both cells. An overview of the cell cycle and mito- sis: during prophase the chromosomes condense, during metaphase the chromo- somes line up, during anaphase the sister chromatids are pulled to opposite sides of the cell, and during telophase the nuclear envelope forms. This is a representation of dividing plant cells. Cell division in plant cells differs slightly from animal cells as a cell wall must form. Note that most of the cells are in interphase. Can you find examples of the different stages of mitosis?
Output: what holds the two chromatids together?
The Premier Mine is an underground diamond mine owned by Petra Diamonds.
Output: What type of product does Premier Mine produce?
In 2001, MicroProse ceased to exist as an entity and Hasbro Interactive sold the MicroProse intellectual properties to Infogrames Entertainment, SA. The MicroProse UK's former main office in Chipping Sodbury was closed in 2002, followed by the company's former headquarters in Hunt Valley, Maryland in 2003.
Output: What year did MicroProse dissolve?
Although it is now seen as belonging to the Italian Nouvelle Vague, Before the Revolution did not attract large audiences in Italy where it only received lukewarm approval from most of the critics.
Output: Which place does Before the Revolution exist in?
A solar eclipse occurs when the new Moon passes directly between the Earth and the Sun (Figure 1.1). This casts a shadow on the Earth and blocks Earths view of the Sun. A total solar eclipse occurs when the Moons shadow completely blocks the Sun (Figure 1.2). When only a portion of the Sun is out of view, it is called a partial solar eclipse. Solar eclipses are rare and usually only last a few minutes because the Moon casts only a small shadow (Figure 1.3). As the Sun is covered by the Moons shadow, it will actually get cooler outside. Birds may begin to sing, and stars will become visible in the sky. During a solar eclipse, the corona and solar prominences can be seen. A solar eclipse occurs when the Moon passes between Earth and the Sun in such a way that the Sun is either partially or totally hidden from view. Some people, including some scientists, chase eclipses all over the world to learn or just observe this amazing phenomenon. A solar eclipse shown as a series of pho- tos. Click image to the left or use the URL below. URL: A lunar eclipse occurs when the full moon moves through Earths shadow, which only happens when Earth is between the Moon and the Sun and all three are lined up in the same plane, called the ecliptic (Figure 1.4). In an eclipse, Earths shadow has two distinct parts: the umbra and the penumbra. The umbra is the inner, cone-shaped part of the shadow, in which all of the light has been blocked. The penumbra is the outer part of Earths shadow where only part of the light is blocked. In the penumbra, the light is dimmed but not totally absent. A total lunar eclipse occurs when the Moon travels completely in Earths umbra. During a partial lunar eclipse, only a portion of the Moon enters Earths umbra. Earths shadow is large enough that a lunar eclipse lasts for hours and can be seen by any part of Earth with a view of the Moon at the time of the eclipse (Figure 1.5). A lunar eclipse does not occur every month because Moons orbit is inclined 5-degrees to Earths orbit, so the two bodies are not in the same plane every month.
Output: during a solar eclipse
China will soon put its first domestically developed electric aircraft into mass production, and designers expect a huge market at home and abroad. "The RX1E is scheduled to receive its airworthiness(,,)certificate from the Civil Aviation Administration of China before the end of this year, with production expected to start next year," Yang Fengtian, an academic at the Chinese Academy of Engineering told China Daily on Thursday. "This will be the first electric aircraft to be certified by our civil aviation authority. The success of the plane means China has become a technology leader in this filed," he added. An electric aircraft runs on electric motors, with electricity coming from fuel cells, solar cells, ultracapacitors, power beaming or batteries. Most of today's electric aircraft are experimental demonstration models. The first manned, electrically powered flight was made in 1973. "We plan to reach an annual production of 100 such aircraft within three years, and the construction of a manufacturing plant that can make 100 RX1Es each year has begun in Shenyang," Yang said, adding that the plane, together with a set of batteries, will sell for nearly 1 million yuan ($163,000). The convenience of use, easy maintenance, low operating costs, high safety levels and eco-friendliness will bring the RX1E many buyers to serve a wide range of purposes, such as police patrols, flight training, entertainment and mapping surveys, he added. Yang said he expects that the Chinese market will need at least 1,000 RX1Es to meet the demand from general aviation businesses. With a maximum takeoff weight of 480 kg and a cruise speed of up to 160 kilometers per hour, the RX1E can fly 90 minutes based on a full charging of its batteries. It is able to carry loads for 230 km and fly at an altitude of nearly 3,000 meters. Yang said, "In addition to the domestic market, _ ."
Output: In which section is this article most likely to be found in a newspaper?
Phil White has just returned from an 18,000-mile, around-the-world bicycle trip.White had two reasons for making this _ journey.First of all, he wanted to use the trip to raise money for charity, which he did.He raised PS70,000 for the British charity, Oxfam.White's second reason for making the trip was to break the world record and become the fastest person to cycle around the world.He is still waiting to find out if he has broken the record or not. White set off from Trafalgar Square, in London, on 19th June 2004 and was back 299 days later.He spent more than l,300 hours in the saddle and destroyed four sets of tyres and three bike chains.He had the adventure of his life crossing Europe, the Middle East, India, Asia, Australia, New Zealand and the Americas.Amazingly, he did all of this with absolutely no support team.No jeep carrying food, water and medicine.No doctor.Nothing! Just a bike and a very, very long road. The journey was lonely and desperate at times.He also had to fight his way across deserts, through jungles and over mountains.He cycled through heavy rains and temperatures of up to 45 degrees, all to help people in need.There were other dangers along the road.In Iran, he was chased by armed robbers and was lucky to escape with his life and the little money he had.The worst thing that happened to him was having to cycle into a headwind on a road that crosses the south of Australia.For l,000 kilometres he battled against the wind that was constantly pushing him.This part of the trip was slow, hard work and depressing, but he made it in the end.Now Mr.White is back and intends to write a book about his adventures.
Output: What actually inspired Phil White to overcome all the difficulties?
Min and Bill is a 1930 American Pre-Code comedy-drama film starring Marie Dressler and Wallace Beery and based on Lorna Moon's novel Dark Star, adapted by Frances Marion and Marion Jackson.
Output: The Min and Bill is based upon what?
The blockage of transcription elongation by RNA polymerase II (pol II) at a DNA damage site on the transcribed strand triggers a transcription-coupled DNA repair (TCR), which rapidly removes DNA damage on the transcribed strand of the expressed gene and allows the resumption of transcription. To analyze the effect of UV-induced DNA damage on transcription elongation, an in vitro transcription elongation system using pol II and oligo(dC)-tailed templates containing a cyclobutane pyrimidine dimer (CPD) or 6-4 photoproduct (6-4PP) at a specific site was employed. The results showed that pol II incorporated nucleotides opposite the CPD and 6-4PP and then stalled. Pol II formed a stable ternary complex consisting of pol II, the DNA damage template, and the nascent transcript. Furthermore, atomic force microscopy imaging revealed that pol II stalled at the damaged region. These findings may provide the basis for analysis of the initiation step of TCR.
Output: Which gene strand is targeted by transcription-coupled repair (TCR)?
Did you know that many people today are getting extra work by taking translating jobs online? It's true! And you can do it too. Many companies in the USA need translators now to translate simple documents online from Chinese to English, such as emails, website content, movie subtitles and more. Companies like Real Translator Jobs are actively seeking translators from China to fill demand. There are more translation jobs than people to complete them. This leaves a big opportunity for anyone to start doing this and earn extra money. Do you have to speak perfect English? No! Even basic knowledge of English is OK. This is really a job for anyone from China who wants to do it. We spoke to one student from Beijing, Harris, who has been doing this for 2 years now and is earning between $150 and $225 every day. He completes about 3 to 4 jobs per day which takes him about 3 hours. It's an incredible amount of money to earn on your spare time! It is great that we now live in the "Information Age" and opportunities like this are available to people in China to earn extra money working from home over the Internet. It's something that our parent's generation could have never imagined. This type of work is surely to do great things for young people in China. If you would also like to do this, it's easy to get started. Just follow these steps: 1. Sign up at Real Translator Jobs (where Chinese translators are needed now), or a similar service. 2. Review the job posting and complete the ones you like. 3. Get paid once per month by cheque, PayPal or bank wire transfer. I hope you enjoyed this information and good luck earning extra money online translating documents.
Output: What's the best title of the passage?
Online clothes shoppers will be told exactly what size is best. for them using new software which combines with their web camera or smart phone to form a"3D tape measure". Scientists from Surrey University and design experts from the London College of Fashion are developing a program which can take precise waist, hip, chest and other measurements from camera images. Using the person's height as a starting point,the software will be able to build up a 3D image and estimate their size at various different points on the body, based on their overall proportions. The result will be a more accurate sizing guide than previous systems based on waist size or a "small/medium/large"scale, which rely on limited measurements and the buyer's perception of their own body size. Shoppers and retailers who choose to sign up for the project could save millions of pounds a year in postage costs by removing the need to order multiple sizes of the same garment and send back ill-fitting clothes, researchers claimed. The project, sponsored by the Engineering and Physical Sciences Research Council(EPSRC), is expected to be brought to market within two years. After choosing an item, the shopper will activate the software, enter their height, stand in front of their webcam or smart phone in their underwear and take a photograph. The software will not store or transmit the image to the internet, but will use it to judge the person's dimensions at various points on their body. Philip Delamore of the London College of Fashion said:"The potential benefits for the fashion industry and for shoppers are huge. Currently, it's common for online shoppers to order two or three different sizes of the same item of clothing at the same time, as they're unsure which one will fit best."
Output: What is the essential part of a 3D tape measure?
Chediak-Higashi syndrome (CHS) is a rare autosomal recessive disorder characterized by immunodeficiency, neurological dysfunction, and oculocutaneous albinism. Recently, several clinical CHS phenotypes have been reported. Here, we report results of a nationwide survey performed to clarify clinical characteristics and outcomes of CHS patients in Japan. Questionnaires were sent to 287 institutions to collect data regarding CHS patients diagnosed between 2000 and 2010, including results of lysosomal trafficking regulator (LYST) gene analysis. Cytotoxicity and degranulation activity of cytotoxic T lymphocytes were analyzed in available patient samples. A total of 15 patients diagnosed with CHS were eligible for enrollment in this study. Of these, 10 (67%) had recurrent bacterial infections, five (33%) developed life-threatening hemophagocytic lymphohistiocytosis (HLH), and one patient had complicated malignant lymphoma. Hematopoietic stem cell transplantation (HSCT) was performed for six patients including three with HLH, and 10 of the enrolled patients have survived at the time of this writing. LYST analysis was performed for 10 patients; seven different mutations were detected in seven patients, whereas no mutation was identified in three patients. Cytotoxicity and degranulation activity were impaired in patients with and without LYST mutation. Results of this survey indicate that one or two patients with CHS were newly diagnosed each year in Japan. The incidence of HLH was not as high as expected. Mutations of genes other than LYST were suspected in some cases. We conclude that determining indication for HSCT for CHS patients should be based on genetic and cytotoxic analysis.
Output: Which mutated gene causes the Chédiak–Higashi Syndrome?
After their season-opening loss to the Chargers, the Bears rebounded by winning their home opener against the Kansas City Chiefs. Bernard Berrian fumbled on the team's opening drive, but a staunch defensive effort held the Chiefs at bay, and eventually allowed the Bears to engineer a successful drive. John St. Clair, a reserve offensive tackle, scored the Bears' first offensive touchdown of the season from a one-yard pass from Rex Grossman. The team forced the Chiefs to punt on their next drive, which was returned for a touchdown by Devin Hester.The Chiefs scored their first points of the game after Damon Huard threw a sixteen-yard touchdown pass to Dwayne Bowe. During halftime, the Bears received the Pro Team Community Award for their charity efforts. The Bears' defense held the Chiefs to only three more points, after forcing pivotal turnovers in two red zone situations and also a blocked field goal attempt. Pro Bowlers Brian Urlacher, Lance Briggs, and Tommie Harris each recorded sacks, and forced Larry Johnson and Huard to leave the game with injuries. Cedric Benson recorded his first one hundred-yard rushing game. With 20-10 win, the Bears advanced to 1-1 record. Q2 - CHI - 10:29 - 2-yard TD pass from Rex Grossman to John St. Clair (Robbie Gould kick) (CHI 7-0) Q2 - CHI - 9:33 - Devin Hester 73-yard punt return TD (Gould kick) (CHI 14-0) Q2 - CHI - 2:30 - Robbie Gould 47-yard FG (CHI 17-0) Q2 - KC - 1:24 - 16-yard TD pass from Damon Huard to Dwayne Bowe (Dave Rayner kick) (CHI 17-7) Q3 - CHI - 8:36 - Robbie Gould 38-yard FG (CHI 20-7) Q3 - KC - 3:12 - Dave Rayner 45-yard FG (CHI 20-10)
Output: Who kicked the longest field goal?
You must have been troubled by when to say "I love you" because it is one of the greatest puzzles in our life. What if you say it first and your partner doesn't love you back? Or if they do say it but you don't feel they mean it? Being the first to declare your love can be never racking and risky and can leave you feeling as vulnerable as a turtle with no shell. But is the person who says it first really in a position of weakness? Doesn't it pay to hold back, play it cool and wait until the other half has shown their hand faster? "A really good relationship should be about being fair and being equal," says psychologist Sidney Crown. "But love is seldom equal." "All relationships go through power struggles but," he says, "if a love imbalance continues for years, the rot will set in." That feeling of "I've always loved you more" may be subverted (,) for a time, but it never goes away completely and it often emerges in squabbling . In love, at least, the silent, withholding type is not always the most powerful. "The strongest one in a relationship is often the person who feels confident enough to talk about their feelings," says educational psychologist Ingrid Collins. Psychosexual therapist Paula Hall agrees. "The one with the upper hand is often the person who takes the initiative." In fact, the person who says "I love you" first may also be the one who says "I'm bored with you' first." Hall believes that much depends on how "I love you" is said and the motivation of the person saying it. Is it said when they're drunk? Is it said before their partner flies off on holiday, and what it really means is "Please don' t be unfaithful to me" ? By saying "I love you", they are really saying "Do you love me?" If so, wouldn't it just be more honest to say that. Collins agrees that intention is everything. "It's not what is said, but how it's said. What it comes down to is the sincerity of the speaker."
Output: What is the most important for you to consider when somebody say "I love you" to you?
Transportes Aereos Guatemaltecos (TAG) is a private passenger and cargo airline based at Guatemala La Aurora International Airport.
Output: What airport is Transportes Aéreos Guatemaltecos part of?
The Stadio Olimpico is the home of San Marino Calcio in Serravalle and was constructed in 1969.
Output: What stadium was San Marino Calcio?
Athole Shearer (November 20, 1900 -- March 17, 1985) was an actress most noted as the sister of motion picture star Norma Shearer and film sound engineer Douglas Shearer.
Output: Who is Athole Shearer's sister?
A dash of style with loads of attitude and a hunger to reach the stars, Lafangey Parindey is about a group of youngsters living in the backstreets of Mumbai. It is an unusual love story of Nandu (Neil Nitin Mukesh) and Pinky (Deepika Padukone) whose path to love is paved by friendship, grit and hope.He is wild. He is born to fight. He is One-Shot Nandu. For the raw, champion street fighter, one-shot is literally what it takes. Absolutely wild, fierce, and hungry to win, this fighter takes boxing to a new level by knocking down his opponents BLINDFOLDED. Needless to say, One-Shot lives life on his own terms and is a local hero amongst his friends. But all that soon changes, when he meets Pinky Palkar.She is blind. She is born to fly. She is Pinky Palkar. A unique girl, a powerhouse of talent! On one hand she works at a regular, boring 9-to-5 job at a mall but on the other hand, she is a kickass dancer on skates! Strong headed, talented and fiercely ambitious these traits make up Pinky Palkar's deadly personality. Her life's ambition is only one to rise above all the 'losers' living in her locality and carve a niche for herself. She will use her talent to show the others that she can win in life. There is only one, tiny, obstacle She is blind.Lafangey Parindey. Two different personalities. Two different lives. Destined to meet.Set in the grungy streets of Mumbai, Lafangey Parindey is an unusual, edgy love story about the never say die spirit even when your last chip is down. It is an intriguing journey of a blindfolded street fighter and a blind dancer who, along with four friends, set out to achieve the impossible.Born wild, will one-shot Nandu ever learn to love? Born to fly, will Pinky Palkar ever learn to see?
Output: Who knocks Pinky down in a freak car accident?
Aleksandr Andreyevich Samokhvalov (1894--1974) was a Russian painter and graphic artist.
Output: What was the date of birth of Aleksandr Andreyevich Samokhvalov?
James Aloysius O'Gorman, Sr. (May 5, 1860 -- May 17, 1943) was a one-term United States Senator from New York.
Output: Which position was held by James Aloysius O'Gorman?
Dendritic cells (DCs), professional antigen-presenting cells with the unique ability to initiate primary T-cell responses, are present in atherosclerotic lesions where they are exposed to oxidative stress that generates cytotoxic reactive oxygen species (ROS). A large body of evidence indicates that cell death is a major modulating factor of atherogenesis. We examined antioxidant defence systems of human monocyte-derived (mo)DCs and monocytes in response to oxidative stress. Oxidative stress was induced by addition of tertiary-butylhydroperoxide (tert-BHP, 30 min). Cellular responses were evaluated using flow cytometry and confocal live cell imaging (both using 5-(and-6)-chloromethyl-2,7-dichlorodihydrofluorescein diacetate, CM-H(2)DCFDA). Viability was assessed by the neutral red assay. Total RNA was extracted for a PCR profiler array. Five genes were selected for confirmation by Taqman gene expression assays, and by immunoblotting or immunohistochemistry for protein levels. Tert-BHP increased CM-H(2)DCFDA fluorescence and caused cell death. Interestingly, all processes occurred more slowly in moDCs than in monocytes. The mRNA profiler array showed more than 2-fold differential expression of 32 oxidative stress-related genes in unstimulated moDCs, including peroxiredoxin-2 (PRDX2), an enzyme reducing hydrogen peroxide and lipid peroxides. PRDX2 upregulation was confirmed by Taqman assays, immunoblotting and immunohistochemistry. Silencing PRDX2 in moDCs by means of siRNA significantly increased CM-DCF fluorescence and cell death upon tert-BHP-stimulation. Our results indicate that moDCs exhibit higher intracellular antioxidant capacities, making them better equipped to resist oxidative stress than monocytes. Upregulation of PRDX2 is involved in the neutralization of ROS in moDCs. Taken together, this points to better survival skills of DCs in oxidative stress environments, such as atherosclerotic plaques.
Output: What type of enzyme is peroxiredoxin 2 (PRDX2)?
Hillside Cannibals is a 2006 American horror film directed by Leigh Scott and produced by The Asylum.
Output: The production company responsible for Hillside Cannibals is what?
In 2000, the label moved from Brussels to London and was renamed PIAS Recordings.
Output: What city is the band PIAS Recordings from?
The Battle of Weihaiwei (Japanese: Ikaiei-no-tatakai () was a battle of the First Sino-Japanese War. It took place between 20 January and 12 February 1895 in Weihai, Shandong Province, China between the forces of the Empire of Japan and Empire of China.
Output: On what date did Battle of Weihaiwei end?
St. Canute's Cathedral (Danish: Odense Domkirke or Sct. Knuds Kirke), also known as Odense Cathedral, is named after the Danish king Canute the Saint (Danish: Knud den Hellige), otherwise Canute IV. It is a fine example of Brick Gothic architecture.
Output: The art style of St. Canute's Cathedral is what?
In America, when the eighth graders leave the middle school, they are often worried about moving to the high school. It is a hard time for them, but it can be an exciting one as well. To make it easier, students need to get familiar with their school. Even though they've done this already in the middle school, it's still important to find where their classrooms are. Most schools take students to the high school for a visit at the end of their eighth grade year. And, most schools also have a freshman speech for parents and students before the first year begins. Sometimes the main office will give away a map of the school. This can help students and parents to find different places in the school. One way to know your high school more and make new friends is to join a club or play a sport. Schools often offer chances to join different clubs and sports. Fall activities begin in August before the first day of school. If you are not interested in sports, there are clubs for any interest, such as drama, dance, chess, photography, community service, etc. These clubs allow students from different grades. By joining an activity, students can find new friendships, not to mention improving their chances in future college applications . The school office will have a list of activities offered at the school and information on how to join them. What's more, to have a great start to a high school year, students can write down some of their worries, and ask for help from teachers and school workers.
Output: When do many schools take students to visit their new high school?
This past Christmas season, I went to visit my parents. During the visit, I found the letters written by my parents to each other during the war in the attic . The letters were piles high,dirty and had not been touched for decades. I asked Mother and Father if I could take the lettersback to my home. They agreed.As I opened each letter, all of them delicate with age, I discovered a new page in this private part of my parents' lives. My father served in the army. His letters were full of frontline descriptions, and they continued all the way through the battle. Each of my mother's letters was sealed with a lipstick kiss. Father wrote that he sealed his return letters by rekissing her lipstick kiss. How they had been missing each other! I finished reading six months of the letters and discovered there were at least eleven months missing. Maybe they were lost forever. Not long after our Christmas visit, Father became very ill and was in hospital. I went to the hospital to see him. As I sat by his bedside, he told me how much receiving those lipstick-kissed letters had meant to him when he had been so far from home. Later that evening, Mother and I revisited the attic in search of the lost letters. Finally we dug them out of Mother's old college trunk. The next day was Valentine's Day, and we went to the hospital. At my father's bedside, I showed him an old envelope. His curiosity was aroused. When he carefully opened the letter, he recognized it and his eyes were filled with tears. He read the love messages that had been delivered years before to my mother in a quavery voice. This Valentine's Day, we were lucky that we had everything.
Output: How did the author's father feel when he saw the letter in hospital?
In eukaryotes, co-translational insertion of selenocysteine into selenoproteins necessitates the participation of the selenocysteine insertion sequence (SECIS), an element lying in the 3'-untranslated region of selenoprotein mRNAs. We report a detailed experimental study of the secondary structures of the SECIS elements of three selenoprotein mRNAs, the rat and human type I iodothyronine deiodinase (5'DI) and rat glutathione peroxidase (GPx). Based on RNase and chemical probing, a new secondary structure model is established. It is characterized by a stem-loop structure, comprising two helices (I and II) separated by an internal loop, with an apical loop surmounting helix II. Sequence comparisons of 20 SECIS elements, arising from 2 5'DI, 13 GPx, 2 selenoprotein P, and 1 selenoprotein W mRNAs, confirm the secondary structure model. The most striking finding of the experimental study concerns a set of conserved sequences in helix II that interact to form a novel RNA structural motif consisting of a quartet composed of non-Watson-Crick base pairs 5'UGAY3': 5'UGAU3'. The potential for forming the quartet is preserved in 15 SECIS elements, but three consecutive non-Watson-Crick base pairs can nevertheless form in the other five SECIS, the central G.A tandem being invariant in all cases. A 3D model, derived by computer modeling with the use of the solution data, suggests that the base pairing interactions in the G.A tandem are of the type found in GNRA loops. The 3D model displays the quartet lying in an accessible position at the foot of helix II, which is bent at the internal loop, suggesting that the non-Watson-Crick base pair arrangement provides an unusual pattern of chemical groups for putative ligand interaction.
Output: What is the name of the stem loop present in the 3' end of genes encoding for selenoproteins?
What determines if a baby is a male or female? Recall that you have 23 pairs of chromosomesand one of those pairs is the sex chromosomes. Everyone has two sex chromosomes. Your sex chromosomes can be X or Y. Females have two X chromosomes (XX), while males have one X chromosome and one Y chromosome (XY). If a baby inherits an X chromosome from the father and an X chromosome from the mother, what will be the childs sex? The baby will have two X chromosomes, so it will be female. If the fathers sperm carries the Y chromosome, the child will be male. Notice that a mother can only pass on an X chromosome, so the sex of the baby is determined by the father. The father has a 50 percent chance of passing on the Y or X chromosome, so there is a 50 percent chance that a child will be male, and there is a 50 percent chance a child will be female. This 50:50 chance occurs for each baby. A couples first five children could all be boys. The sixth child still has a 50:50 chance of being a girl. One special pattern of inheritance that doesnt fit Mendels rules is sex-linked inheritance, referring to the inher- itance of traits that are located on genes on the sex chromosomes. Since males and females do not have the same sex chromosomes, there will be differences between the sexes in how these sex-linked traitstraits linked to genes located on the sex chromosomesare expressed. Sex-linked traits usually refer to traits due to genes on the X chromosome. One example of a sex-linked trait is red-green colorblindness. People with this type of colorblindness cannot tell the difference between red and green. They often see these colors as shades of brown ( Figure 1.1). Boys are much more likely to be colorblind than girls ( Table 1.1). This is because colorblindness is a sex-linked, recessive trait. Boys only have one X chromosome, so if that chromosome carries the gene for colorblindness, they will be colorblind. As girls have two X chromosomes, a girl can have one X chromosome with the colorblind gene and one X chromosome with a normal gene for color vision. Since colorblindness is recessive, the dominant normal gene will mask the recessive colorblind gene. Females with one colorblindness allele and one normal allele are referred to as carriers. They carry the allele but do not express it. How would a female become colorblind? She would have to inherit two genes for colorblindness, which is very unlikely. Many sex-linked traits are inherited in a recessive manner. Xc Xc X (carrier female) Xc Y (colorblind male) X Y X XX (normal female) XY (normal male) According to this Punnett square ( Table 1.1), the son of a woman who carries the colorblindness trait and a male with normal vision has a 50% chance of being colorblind.
Output: most sex-linked genes are ___________.
Acute graft-versus-host disease (GvHD) limits the applicability of allogeneic hematopoietic cell transplantation for the treatment of leukemia. GvHD occurs as a consequence of multiple activating events in antigen-presenting cells (APCs) and T cells (Tcs). Spleen tyrosine kinase (Syk) is an intracellular non-receptor tyrosine kinase involved in multiple signaling events of immune cells. Therefore, we hypothesized that Syk may be a promising target to inhibit GvHD, which involves activation of different immune cell populations. In vivo expansion of luciferase(+) donor Tcs in mice developing GvHD was reduced by treatment with the Syk inhibitor Fostamatinib, which led to increased survival and reduced histologically confirmed GvHD severity. Importantly, in vivo and in vitro cytotoxicity against leukemia target cells and anti-murine cytomegalovirus immune responses were not impacted by Fostamatinib. In APCs Syk inhibition reduced the expression of costimulatory molecules and disrupted cytoskeletal organization with consecutive APC migratory defects in vitro and in vivo while phagocytic activity remained intact. On the basis of these immunomodulatory effects on different cell populations, we conclude that Syk targeting in alloantigen-activated Tcs and APCs with pharmacologic inhibitors, already applied successfully in anti-lymphoma therapy, has clinical potential to reduce GvHD, especially as anti-leukemia and anti-viral immunity were preserved.
Output: Which enzyme is inhibited by a drug fostamatinib?
The Province of Brabant was a province in Belgium from 1830 to 1995.
Output: What year did Province of Brabant end?
Shprintzen-Goldberg syndrome (SGS) is characterized by craniosynostosis and marfanoid habitus. The clinical findings of SGS include neurological, cardiovascular, connective tissue, and skeletal abnormalities. Among these skeletal findings, developmental scoliosis is recognized in half of all patients with SGS. However, no earlier reports have described the surgical treatment of scoliosis associated with SGS. Four patients (2 boys and 2 girls; mean age at the time of surgery, 7.34.4 y) with SGS who underwent surgical treatment for progressive scoliosis were reviewed. The radiologic findings, operative findings, and perioperative complications were evaluated. The mean preoperative Cobb angle was 102.816.9 degrees. The curve patterns were a double curve in 2 cases and a triple curve in 2 cases. Local kyphosis at the thoracolumbar area was recognized in all the cases with a mean kyphosis angle of 4916 degrees. Growing rod procedures were performed in 2 patients, and posterior correction and fusion were performed in 2 patients. The mean correction rate was 45% in the patients who underwent the growing rod procedures at the time of growing rod placement and 51% in the patients who underwent posterior correction and fusion. Dislodgement of the proximal anchors occurred in 3 of the 4 patients. One patient developed pseudoarthrosis. Two patients developed deep wound infections, and implant removal was necessary in 1 patient. Surgical treatment for scoliosis in patients with SGS was associated with a high incidence of perioperative and postoperative complications including implant dislodgements and deep wound infections attributable to poor bone quality and a thin body habitus, which are characteristic clinical features of this syndrome. Careful preoperative surgical planning and postoperative care are critical for the surgical treatment of scoliosis associated with SGS, especially in infants requiring multiple surgeries. Level IV.
Output: Which disease is included as an additional feature in the Goldberg-Shprintzen syndrome?
Miles Monroe (Woody Allen), a jazz musician and owner of the "Happy Carrot" health-food store in 1973, is subjected to cryopreservation without his consent, and not revived for 200 years.[2] The scientists who revive him are members of a rebellion: 22nd-century America seems to be a police state, ruled by a dictator about to implement a secret plan known as the "Aries Project". The rebels hope to use Miles as a spy to infiltrate the Aries Project, because he is the only member of this society without a known biometric identity. The authorities discover the scientists' project, and arrest them. Miles escapes by disguising himself as a robot, and goes to work as a butler in the house of socialite Luna Schlosser (Diane Keaton). When Luna decides to have his head replaced with something more "aesthetically pleasing," Miles reveals his true identity to her, whereupon Luna threatens to give Miles to the authorities. In response, he kidnaps her and goes on the run, searching for the Aries Project. Miles and Luna fall in love, but Miles is captured and brainwashed into becoming a complacent member of the society, while Luna joins the rebellion. The rebels kidnap Miles and perform reverse-brainwashing, whereupon he remembers his past and joins their efforts. Miles becomes jealous when he catches Luna kissing the rebel leader, Erno Windt (John Beck), and she tells him that she believes in free love. Miles and Luna infiltrate the Aries Project, wherein they quickly learn that the national leader had been killed by a rebel bomb ten months previously. All that survives is his nose. Other members of the Aries Project, mistaking Miles and Luna for doctors, expect them to clone the leader from this single remaining part. Miles steals the nose and "assassinates" it by dropping it in the path of a road roller. After escaping, Miles and Luna debate their future together. He tells her that Erno will inevitably become as corrupt as the Leader. Miles and Luna confess their love for one another, but she claims that science has proven men and women cannot have meaningful relationships due to chemical incompatibilities. Miles dismisses this, saying that he does not believe in science, and Luna points out that he does not believe in God or political systems either. Luna asks Miles if there is anything he does believe in, and he responds with the line, "Sex and deathtwo things that come once in a lifetimebut at least after death you're not nauseous."[3] The film ends as the two embrace.
Output: What kind of music does Miles play?
Arcadi Balaguer i Costa was president of FC Barcelona between 1925 and 1929.
Output: Who led Arcadi Balaguer?
McLeod syndrome is caused by mutations of XK, an X-chromosomal gene of unknown function. Originally defined as a peculiar Kell blood group variant, the disease affects multiple organs, including the nervous system, but is certainly underdiagnosed. We analyzed the mutations and clinical findings of 22 affected men, aged 27 to 72 years. Fifteen different XK mutations were found, nine of which were novel, including the one of the eponymous case McLeod. Their common result is predicted absence or truncation of the XK protein. All patients showed elevated levels of muscle creatine phosphokinase, but clinical myopathy was less common. A peripheral neuropathy with areflexia was found in all but 2 patients. The central nervous system was affected in 15 patients, as obvious from the occurrence of seizures, cognitive impairment, psychopathology, and choreatic movements. Neuroimaging emphasized the particular involvement of the basal ganglia, which was also detected in 1 asymptomatic young patient. Most features develop with age, mainly after the fourth decade. The resemblance of McLeod syndrome with Huntington's disease and with autosomal recessive chorea-acanthocytosis suggests that the corresponding proteins--XK, huntingtin, and chorein--might belong to a common pathway, the dysfunction of which causes degeneration of the basal ganglia.
Output: Mutation of which gene is associated with McLeod syndrome?
Wang Jungzhi (Wang Ch'un Ch'ih) was one of the last people to be executed by the British Army in World War I. He was killed by firing squad on 8 May 1919, six months after the Armistice after being convicted of murder.
Output: Which crime Wang Jungzhi is considered to have commited?
Later he would travel to Basra in Iraq to study under Hasan al-Basri (one of the Tabi''in). In Basra he began to develop the ideologies that would lead to the Mutazilite school. These stemmed from conflicts that many scholars had in resolving theology and politics. His main contribution to the Mutazilite school was in planting the seeds for the formation of its doctrine. Wasil ibn Ata died in 748 in the Arabian Peninsula. He married the sister of Amr ibn Ubayd.
Output: What is Wasil ibn Ata's place of employment?
General Sir Alfred Dudley Ward GCB, KBE, DSO (27 January 1905--28 December 1991), was a senior officer of the British Army who saw service during World War II and later became Governor of Gibraltar.
Output: What branch was Alfred Dudley Ward part of?
Obazine Abbey, also known as Aubazine Abbey, was a Cistercian monastery in the present town of Aubazine in the departement of Correze in the Limousin in France.
Output: To which religious order did Obazine Abbey belong?
Tsarevich Dmitry or Dmitri Ivanovich (Russian: , tr. Dmitrii Ivanovich; 19 October 1582 -- 15 May 1591), also known as Dmitry of Uglich ( , Uglichskii) or Dmitry of Moscow ( , Moskovskii), was a Russian tsarevich famously impersonated by a series of pretenders after the death of his father Ivan the Terrible.
Output: Who is listed as Dmitry of Uglich father?
The Reichswehr (English: Empire Defence) formed the military organization of Germany from 1919 until 1935, when it was united with the newly founded Wehrmacht (''Defence Force'').
Output: What year did Reichswehr dissolve?
Publius Minucius Augurinus (Latin, Publius Minucius Augurinus ) was a Roman Republican politician of the patrician gens Minucia during the beginning of the 5th century BC. He served as Consul of Rome in 492 BC
Output: What city was Publius Minucius Augurinus born in?
Shirley Temple Black, who lifted America's spirit as a bright-eyed child movie star during the Great Depression, peacefully passed away at her California home on Monday evening at 85, surrounded by her family and caregivers. Temple, born on April 23, 1928, started her entertainment career in the early 1930s and was famous by age 6. Temple was 3 when her mother put her in dance school, where a talent scout spotted her and got her in Baby Burlesks, a series of short movies with child actors playing in adult movies. Movie studio directors took notice of her and in 1934 she appeared in the film Stand Up and Cheer! and her song and dance caught people's attention. Movies such as Little Miss Marker and Bright Eyes featured her signature song. In 1935, she received a special Oscar for her "outstanding contribution to screen entertainment" in the movie Stand Up and Cheer! She made some 40 feature movies, including The Little Colonel, Poor Little Rich Girl, Heidi and Rebecca of Sunnybrook Farm, in 10 years, starring with big-name actors like Randolph Scott, Lionel Barrymore and Jimmy Durante. Temple was a superstar before the term was invented. She said she was about 8 when crowds shouting their love for her made her realize she was famous. "I wondered why," she recalled. "I asked my mother and she said, 'Because your films make them happy.' " Her child career came to an end at 12. She tried a few roles as a teenager--including opposite future president Ronald Reagan in That Hagen Girl - but retired from the screen in 1949 at 21. Temple was only 17 when she married for the first time to John Agar, who would eventually appear with her in two movies. Their five-year marriage produced a daughter. In 1950 she wed Charles Black in a marriage that lasted until his death in 2005. She and Black had two children. Temple's interest in politics was sparked in the early 1950s when her husband was called back into the Navy to work in Washington.
Output: For which movie did Shirley Temple win the Oscar?
CC's (pronounced cee-cees) is a brand of flavoured tortilla chips produced since the early 1980s, originally by The Smith's Snackfood Company, and currently by Snack Brands Australia.
Output: The country for CC's was what?
Empagliflozin is an orally available, potent and highly selective inhibitor of the sodium glucose cotransporter 2 (SGLT2). This study was undertaken to investigate the effect of food on the pharmacokinetics of 25 mg empagliflozin and to assess dose proportionality between 10 mg and 25 mg empagliflozin under fasted conditions. In this open-label, 3-way, cross-over study, 18 healthy volunteers received 3 single doses of empagliflozin in a randomized sequence (25 mg empagliflozin under fasted conditions, 25 mg empagliflozin after a high-fat, high-calorie breakfast and 10 mg empagliflozin under fasted conditions), each separated by a washout period of at least 7 days. Serial plasma samples were collected at selected time points over a period of 72 hours. Administration with food had no clinically relevant effect on the area under the plasma concentration-time curve (AUC0-) of empagliflozin (geometric mean ratio (GMR): 84.04, 90% confidence interval (CI): 80.86 - 87.34). The decrease observed in the maximum plasma concentrations (Cmax) of empagliflozin (GMR: 63.22, 90% CI: 56.74 - 70.44) when administered with food was not considered clinically meaningful. The increases in AUC0- and Cmax for 10 mg vs. 25 mg empagliflozin administered under fasting conditions were roughly dose-proportional, as demonstrated by the slope of the regression lines being slightly less than 1 (slope for AUC0-: 0.94, 95% CI: 0.90 - 0.97; slope for Cmax: 0.91, 95% CI: 0.80 - 1.01). Empagliflozin was well tolerated under fed and fasting conditions. The results support administration of empagliflozin tablets independently of food. Increases in empagliflozin exposure under fasting conditions were roughly dose-proportional between 10 mg and 25 mg empagliflozin.
Output: Which protein does empagliflozin inhibit?