niryuu/sni-each-converted huggingface dataset:
Sickle cell disease : continuous arterial spin - labeling perfusion MR imaging in children .
Output: Sickle cell
The actin - regulatory protein gelsolin is found both intracellularly and in plasma [ 147 , 148 ] .
Output: intracellularly
Bone histology showed changes consistent with osteoblast inhibition by methotrexate .
Output: osteoblast
In an earlier study , we have shown that Pi induced apoptosis of terminally differentiated hypertrophic chondrocytes .
Output: chondrocytes
Based on these observations , we suggest that , at the cartilage mineralization front , an elevation in local environmental Ca2 + and Pi concentrations modulates oxidative metabolism , and triggers apoptosis of terminally differentiated chondrocytes .
Output: chondrocytes
The reaction mix was transformed in E . coli chemically competent cells as described above .
Output: competent cells
Prior studies have shown that striatal VMAT2 density provides an objective estimate of dopaminergic neuronal integrity , without confounding effects of compensatory regulation .
Output: neuronal
These neutrophils demonstrated a consistent deficiency ( 44 + / - 8 % of control values ) in superoxide anion ( O2 - ) production in response to the phorbol ester , phorbol myristate acetate ( PMA ) .
Output: neutrophils
These results suggest that a deficient O2 - release in response to PMA is a hallmark of neutrophils in CNL and may provide a diagnostic indicator of this condition .
Output: neutrophils
To assess the changes in the oxidative capacity of neutrophils after the burn , blood samples for the Phagoburst analysis were taken on admission and at least once every second week for the duration of stay in hospital and thereafter monthly up to 12 months after the burn .
Output: neutrophils
Oxidative capacity of the neutrophils decreased similarly for all three stimulants : there was a pathological decrease shortly after admission , with the lowest value occurring between days 7 and 10 , followed by a gradual recovery during the ensuing months .
Output: neutrophils
This study provides evidence that immunosuppression in those injured by burns , as assessed by the in vitro oxidative burst of neutrophils , remains long after the event of the burn ( up to 3 . 5 months after burn ) .
Output: neutrophils
1 , 10 ng of TORC1 , 5 ng of TORC2 , and 15 ng of PKAalpha expression plasmids were transfected into HEK293 cells .
Output: HEK293 cells
( C ) Confocal images of C - terminally MYC - tagged MOR1 or FLAG - tagged MOR1K overexpressed in HEK293 cells and stained with either Anti - MYC - Tag Antibody ( Alexa Fluor 647 Conjugate ) or Anti - DYKDDDDK Tag Antibody ( Alexa Fluor 555 conjugate ) .
Output: HEK293 cells
Human Langerin - DTA mice constitutively lack LCs and develop exaggerated contact hypersensitivity ( CHS ) responses .
Output: LCs
Thus , LCs appear to be suppressive in human Langerin - DTA mice and redundant in murine Langerin - DTR mice .
Output: LCs
The inducible ablation of LCs in human Langerin - DTR mice resulted in increased CHS .
Output: LCs
Gene expression by in situ hybridization demonstrated a significant increase in IL - 6 and fibrinogen mRNAs in cells surrounding the central vein in sterile and septic animals , being greatest in animals with sepsis , associated with an increased deposition of collagen in the central vein area , most prominent in the septic liver .
Output: cells
The pericentral vein cells with IL - 6 and fibrinogen mRNA increases paralleled the increases in cells containing IL - 6 and fibrinogen mRNAs in the abscess walls of sterile and septic animals , respectively .
Output: cells
We also explored the mechanism of apoptosis by evaluating mitochondrial activity and reactive oxygen species ( ROS ) generation in cells treated with the ion pair .
Output: cells
We reported previously that various radiocontrast media cause apoptosis in porcine proximal tubular ( LLC - PK ( 1 ) ) cells , in which reduction in B - cell lymphoma ( Bcl ) - 2 expression and caspase - 3 activation are implicated .
Output: cells
In the present study , we investigated a role for ceramide in radiocontrast media - induced apoptosis in renal tubular cells .
Output: cells
LLC - PK ( 1 ) cells were exposed to radiocontrast media for 30 min , followed by incubation for 24 h in normal medium .
Output: cells
The CSF - 1 receptor exerts its biological effects on cells via a range of signaling proteins including Erk1 / 2 and Akt .
Output: cells
The second preparation consisted of human IL 1 partially purified from supernatants of human peripheral blood mononuclear cells stimulated with S . albus .
Output: cells
Treatment of the cells with verapamil before and during ouabain exposure failed to inhibit the ouabain - induced increase in cell - associated 45Ca .
Output: cells
Our panel of specific PTPase primers should be very useful for semiquantitatively assessing the repertoire of PTPases expressed by cells .
Output: cells
Neutrophils were stimulated in vitro by Escherichia coli , phorbol 12 - phorbol myristate 13 - acetate ( PMA ) , and peptide N - formyl - Met - Leu - Phe ( fMLP ) .
Output: Neutrophils
It contains approximately 60 hair cells ( measured in Rana catesbeiana ) , from which the stereovilli protrude into the lumen and connect to the tectorial membrane ( Frishkopf and Flock 1974 ) .
Output: hair cells
Typically the orientation of the hair cells , as defined by the direction to which the v - shape of the stereovilli bundle points ( Lewis et al . 1985 ) , is away from the sacculus in Ranidae .
Output: hair cells
The total lymph node cell numbers ( A ) and IL - 4 ( B ) , IFN - gamma ( C ) and IL - 10 ( D ) secreted from the PLN cells after ex vivo stimulation with Con A were determined .
Output: PLN cells
Prolonged control of patterned biofilm formation by bio - inert surface chemistry .
Output: biofilm
A bio - inert surface chemistry was developed that can confine biofilm formation in designed patterns for at least 26 days .
Output: biofilm
Relative phage haplotype frequencies in PAO1 samples , ( A ) planktonic , ( B ) biofilm 4 days , ( C ) biofilm 11 days .
Output: biofilm
Cerebral blood flow ( CBF ) was measured with continuous arterial spin - labeling perfusion magnetic resonance ( MR ) imaging in 14 children with sickle cell disease and seven control subjects .
Output: sickle cell
Baseline CBF was significantly decreased in territories seen as unaffected on conventional MR images and MR angiograms in four children with sickle cell disease .
Output: sickle cell
Determination of eprosartan in human plasma and urine by LC / MS / MS .
Output: LC
A protein precipitation , liquid chromatography / tandem mass spectrometry ( LC / MS / MS ) method has been developed and validated for the determination of eprosartan in human plasma and urine .
Output: LC
Isolated X . laevis oocytes incubated in medium containing 25 microgram DNA / ml labeled with either 125I , 32P or 14C and from three different sources ( bovine , E . coli and X . laevis ) , incorporated the label at an average rate of 0 . 11 ng . mm - 2 . hr - 1 .
Output: oocytes
Our data suggest that yolk - DNA arises by the adventitious uptake of DNA present in the maternal serum by vitellogenic oocytes .
Output: oocytes
Induction of apoptosis in skeletal tissues : phosphate - mediated chick chondrocyte apoptosis is calcium dependent .
Output: chondrocyte
While high levels of Ca2 + alone had little effect on chondrocyte viability , the cation enhanced Pi - dependent cell death and greatly increased Pi uptake .
Output: chondrocyte
( D ) Confocal images of C - terminally FLAG - tagged MOR1K overexpressed in Be2C cells and stained with either Anti - FLAG M2 Antibody Alexa Fluor Conjugate or fluorescent - labeled naloxone ( FNAL ) .
Output: Be2C cells
Be2C cells transfected with either MOR1 or MOR1K isoforms showed increased retention of FNAL at concentrations of 0 . 1 and 1 muM .
Output: Be2C cells
Important parameters are tumor site , depth of invasion , histological type and grade , pattern of invasion ( diffusely infiltrating versus expanding margin ) , degree of peritumoral lymphocytic infiltration , and tumor involvement of surgical margins and lymph nodes .
Output: peritumoral lymphocytic
The defect in antigen presentation induced by UV irradiation of Mo . TT was reversed in a dose - dependent manner by the addition of two different preparations containing human interleukin 1 ( IL 1 ) .
Output: Mo
The first preparation consisted of supernatants of Mo . stimulated with Con A for 18 hr and in which Con A activity was blocked by alpha - D - methyl - mannoside ( Mo . - Con A - Sup ) .
Output: Mo
Mo Kalpha radiation
Output: Mo
Monoclinic , P21 / n Mo Kalpha radiation , lambda = 0 . 71073 A
Output: Mo
Neurons were treated with ephrin - A5 - Fc for 30 min followed by visualization of GFP ( A , C , E ) or CA - MEK - 1 ( green in B , D , F ) , F - actin and betaIII tubulin .
Output: Neurons
This IL 1 copurified with human leukocyte pyrogen ( LP ) and was termed IL 1 / LP .
Output: leukocyte
No evidence for an influence of the human platelet antigen - 1 polymorphism on the antiplatelet effects of glycoprotein IIb / IIIa inhibitors .
Output: platelet
This study investigated the hypothesis that the human platelet antigen - 1 ( HPA - 1 ) polymorphism may influence the antiplatelet effects of glycoprotein ( GP ) IIb / IIIa inhibitors .
Output: platelet
Thus , the present study does not provide evidence for an effect of HPA - 1 polymorphism on the inter - individual variability in the platelet inhibitory effects of the three GPIIb / IIIa inhibitors approved for clinical use .
Output: platelet
Lactotransferrin binding to its platelet receptor inhibits platelet aggregation .
Output: platelet
Inhibition of platelet aggregation was also observed with the N - terminal fragment of lactotransferrin ( residues 3 - 281 ; 50 % inhibition = 2 microM ) and with CFQWQRNMRKVRGPPVSC synthetic octodecapeptide ( residues 20 - 37 ; 50 % inhibition = 20 microM ) corresponding to one of the two external loops ( residues 28 - 34 and 39 - 42 ) where we recently located the receptor - binding site .
Output: platelet
Finally , the inhibition was demonstrated to be mediated by a mechanism which requires the binding of lactotransferrin to its putative receptor and not to platelet glycoprotein IIb - IIIa .
Output: platelet
TxA2 , generated from arachidonic acid by cyclooxygenase 1 ( COX - 1 ) and Tx synthase , further amplifies platelet activation .
Output: platelet
COX - 1 converts arachidonic acid into prostaglandin endoperoxides PGG2 and PGH2 , the latter being , in turn , transformed by Tx synthase into TxA2 , a potent amplifier of platelet aggregation with vasoconstrictive properties .
Output: platelet
At the site of the atheroma rupture , platelet - released TxA2 leads to downstream micro - vessel contraction and thrombus propagation ( 7 ) .
Output: platelet
In addition to its role in coagulation , thrombin at extremely low concentrations is one of the major platelet activators ( 8 ) .
Output: platelet
PAR - 1 and P2Y12 cross - react in platelet activation , and Galphaq and Galphai - coupled receptors are involved in this process .
Output: platelet
Thrombin - dependent platelet aggregation is mediated in part by secreted ADP , acting on the Galphai - linked ADP receptor .
Output: platelet
By blocking Galphaq via PAR - 1 and Gbetai via P2Y12 , combined inhibition of thrombin and P2Y12 receptors leads to a synergistic inhibitory effect on thrombin - induced platelet aggregation ( 10 ) .
Output: platelet
5 - HT is a vasoconstrictor agent that binds to 5HT - 2A receptors and amplifies the platelet response by stimulating shape change and enhancing platelet recruitment at sites of injury ( 3 ) .
Output: platelet
It may also play a pro - coagulant role by promoting the retention of fibrinogen and thrombospondin on the platelet surface .
Output: platelet
Dextransucrase ( EC 2 . 4 . 1 . 5 ) , which catalyses soluble glucan synthesis , was most active in the supernatant fluid from cultures grown with excess glucose , fructose or sucrose , but full activity was detected only when the enzyme was incubated with both sucrose and dextran .
Output: EC
These cultured fibroblasts were characterized using immunocytochemistry .
Output: fibroblasts
These signals were evident in both the pigmented and non - pigmented epithelial cell layers of the pars plicata region of the ciliary processes , but not in the pars plana or in the ciliary musculature .
Output: epithelial cell
The mitochondrial dysfunction was not limited to just the beta cell , as mitochondria from both the liver and skeletal muscle exhibit decreased oxidation of pyruvate , subsequently leading to the development of features commonly found in T2D [ 100 , 121 ] .
Output: beta cell
Chronic rubella viremia has been detected in peripheral blood mononuclear cell ( MNC ) populations in five of the six women up to six years after vaccination .
Output: MNC
The fluorescent probe allowed us to characterize , by flow cytometry , the binding of lactotransferrin to non - activated human platelets .
Output: platelets
Human platelets express two cell surface G - protein - coupled protease - activated receptors ( PARs ) for thrombin : PAR - 1 and PAR - 4 .
Output: platelets
Cell viability was assessed by 2 - ( 2 - methoxy - 4 - nitrophenyl ) - 3 - ( 4 - nitrophenyl ) - 5 - ( 2 , 4 - disulfophenyl ) - 2H - tetrazolium monosodium salt assay , while apoptosis was determined by terminal deoxynucleotidyl transferase - mediated dUTP nick end labeling stain .
Output: Cell
Hall symbol : - P 2yn Cell parameters from 25 reflections
Output: Cell
Molecular Mechanisms Mediating the Perinatal Beta - Cell Adaptive Response to Early - Life Stressors
Output: Cell
Multiple phosphotyrosine phosphatase mRNAs are expressed in the human lung fibroblast cell line WI - 38 .
Output: fibroblast
The authors have used reverse transcription / polymerase chain reactions to accomplish a comprehensive examination of the RNA expression for 58 distinct mammalian protein tyrosine and dual specificity phosphatase ( PTPase ) and PTPase - like genes in the normal human diploid fibroblast cell line WI - 38 .
Output: fibroblast
It was found that 98 % of the cells were vimentin positive and also positive for a monoclonal antibody against specific fibroblast antigen ( clone ASO2 , Dianova , Hamburg , Germany ) .
Output: fibroblast
Long - term immunosuppression in burned patients assessed by in vitro neutrophil oxidative burst ( Phagoburst ) .
Output: neutrophil
To assess the duration and magnitude of immunosuppression induced by burns as measured by the neutrophil oxidative burst in vitro .
Output: neutrophil
Oesophageal candidiasis and croup in a child with defective neutrophil motility .
Output: neutrophil
There had been delayed separation of the umbilical cord and repeated infections associated with a defect of neutrophil motility .
Output: neutrophil
We would emphasis that candidiasis should be anticipated and treated vigorously in children with such a defect of neutrophil motility .
Output: neutrophil
Given the unique NCC origin of PF suture complex ( analogous to metopic suture in humans ) , we performed quantitative real - time PCR and immunohistochemistry to study the expression pattern of the NCC determinant gene Sox9 and select markers of extracellular matrix .
Output: NCC
Moreover , we studied the effect of haploinsufficiency of the NCC determinant gene Sox9 in the NCC derived PF suture complex .
Output: NCC
A decrease in dosage of Sox9 by haploinsufficiency in NCC - derived tissues resulted in delayed PF suture closure .
Output: NCC
Further , the almost identical distribution of the 5 - HT1A and 5 - HT7 messengers ribonucleic acid indicate that the receptors may be co - localized in epithelial cells .
Output: epithelial cells
Here we have investigated the potential involvement of the Src - like adapter protein ( SLAP - 2 ) in signaling by the CSF - 1 receptor in mouse bone marrow - derived macrophages .
Output: macrophages
RT - PCR analysis revealed constitutive expression of the SLAP - 2 gene in bone marrow macrophages .
Output: macrophages
Overexpression of SLAP - 2 in bone marrow macrophages partially suppressed the CSF - 1 - induced tyrosine phosphorylation and / or expression level of a approximately 80 kDa protein without affecting CSF - 1 - induced global tyrosine phosphorylation , or activation of Akt or Erk1 / 2 .
Output: macrophages
In cellular models , we show that the TBX21 variant increases T helper 1 and decreases T helper 2 cytokine expression comparably with wild type .
Output: cellular
Young mice can increase the cellular availability of GSH , whereas mature mice can increase GSH synthetase activity during oxidative stress .
Output: cellular
The primary cellular response in the draining lymph node The primary cellular response in the draining PLN was determined five days after a single injection of HBSS ( open columns ) , 10 mug OVA ( gray ) , 40 mug PSP ( hatched ) or 10 mug OVA + 40 mug PSP ( black ) into both hind footpads of BALB / cA , NIH and C3H / HeN mice .
Output: cellular
They are known to shorten with each cellular division and , hence , can act as a mitotic clock , registering the number of replicative divisions to have taken place within the cell .
Output: cellular
Role of interleukin 1 in antigen - specific T cell proliferation .
Output: T cell
The role of interleukin 1 ( IL 1 ) in human antigen - specific T cell proliferation was examined .
Output: T cell
The reaction mix was then added to 100 microl chemically competent DH10b cells , incubated for 15 - 30 min on ice and transformed by heat shock .
Output: DH10b cells
In the desired in vivo application for this work , as a sensor for stent healing , it can be expected that in the course of normal healing the stent will be fully populated with a significantly thicker layer of endothelial cells .
Output: endothelial cells
Since then , a role of histone methyl transferases , particularly set7 , has also been demonstrated in the sustained deleterious effects of chronic hyperglycemia on human microvascular endothelial cells [ 114 ] .
Output: endothelial cells
In the last several years Listerine has emerged as a bona fide therapeutic agent for reduction of plaque induced oral diseases .
Output: plaque
Moreover , reports by both American and British professional societies have led to a re - evaluation of the relative risks associated with plaque induced bacteremia when twice - yearly visits to dental professionals are compared to daily activities .
Output: plaque
These new recommendations and revelations open the door for local antimicrobial approaches to reduce the challenge of plaque - induced bacteremias .
Output: plaque
Furthermore , when ACT is overexpressed in transgenic mice , an increased plaque load in the brains of these mice and impaired spatial learning is observed [ 134 , 135 ] .
Output: plaque
Both IL 1 - containing preparations enhanced the response of C57BL / 6 mouse thymocytes to phytohemagglutinin .
Output: thymocytes
Defective expression of the granulocyte - macrophage colony - stimulating factor receptor was ruled out .
Output: macrophage
In the fetal GK / Par rat exposed to mild hyperglycemia during gestation ( a model of IUED ) , data from our group suggest that the beta - cell deficit ( reduced by more than 50 % ) starts as early as fetal age E16 and reflects decreased beta - cell proliferation , a limitation of beta - cell neogenesis from precursors , and increased apoptosis of both beta cells and their precursors [ 86 ] .
Output: beta cells
There is also increased hepatocellular lipid deposition and apoptosis .
Output: hepatocellular
Experiments using various types of deafferentation and fetal brain tissue transplantation confirmed that the LHRH pulse generator is located in the MBH and suggested that LHRH pulse generator consists of nonLHRH neurons .
Output: neurons
Subsequently , neurons were subjected to either an ephrin - A5 mediated growth cone collapse assay ( Fig . 5 ) or stimulated with guidance cues for 20 minutes and IEG mRNA levels were quantified via qRT - PCR ( Fig . 6 ) .
Output: neurons
Wild - type neurons were electroporated with vectors expressing GFP ( control ; A , C , E ) or expressing a FLAG - tagged constitutively - active MEK1 ( CA - MEK1 ; B , D , F ) .
Output: neurons
Ephrin - A5 induces a growth cone collapse , resulting in reduced growth cone area and filopodia number in GFP but not CA - MEK1 expressing neurons .
Output: neurons
In mock - electroporated neurons , BDNF induced an IEG response of c - fos ( A ) , Egr1 ( B ) , Egr2 ( C ) and Arc ( D ) .
Output: neurons
Co - application of ephrin - A5 and BDNF reduced mRNA levels of all four IEGs in mock - electroporated neurons ( A - D ) .
Output: neurons
The average neurite length of neurons overexpressing CA - MEK1 ( Fig . 5B ) was clearly reduced compared to control GFP expressing neurons ( Fig . 5A ; see quantification in ( G ) ) .
Output: neurons
Growth cones of GFP ( C ) and CA - MEK1 expressing neurons were similar and protruded many filopodia ( arrows in Fig . 5C - F ) .
Output: neurons
In contrast , in neurons overexpressing CA - MEK1 ( Fig . 5F ) , ephrin - A5 induced only a partial growth cone collapse .
Output: neurons
In agreement with previous results ( Fig . 4 ) , in mock - electroporated neurons , ephrin - A5 incubation reduced the BDNF - mediated up - regulation of c - fos ( Fig . 6A ) , Egr1 ( Fig . 6B ) , Egr2 ( Fig . 6C ) and Arc ( Fig . 6D ) mRNA .
Output: neurons
In contrast , in neurons over - expressing CA - MEK1 , ephrin - A5 could not suppress BDNF - induced IEG responses to the same degree .
Output: neurons
Particularly Egr1 ( Fig . 6B ) and Egr2 ( Fig . 6C ) mRNA levels were almost identical when comparing neurons treated with BDNF alone and neurons with BDNF and ephrin - A5 together .
Output: neurons
In vitro cytotoxic potential and mechanism of action of selected coumarins , using human renal cell lines .
Output: cell lines
In the same way of thinking , studies have demonstrated that the maintenance of methylated histone H3 Lys4 by Set7 / 9 , a member of the SET methyltransferase family , is crucial to Pdx1 activity in beta - cell lines [ 111 - 113 ] .
Output: cell lines
( A , B ) Overexpression of CA - MEK1 ( B ) reduces mean neurite length compared to a control GFP - expressing neuron ( A ) .
Output: neuron