niryuu/sni-each-converted huggingface dataset:
The ineffectiveness of tobramycin combination therapy in Streptococcus faecium endocarditis .
Output: Streptococcus faecium
A patient required mitral valve replacement following ineffective antibiotic treatment of enterococcal endocarditis caused by Streptococcus faecium .
Output: Streptococcus faecium
Against Streptococcus faecium Isolates from Blood Cultures
Output: Streptococcus faecium
' Wennersten C , Moellering RC Jr : Mechanism of resistance to penicillin - aminoglycoside synergism in Streptococcus faecium .
Output: Streptococcus faecium
defective Streptococcus faecium strains .
Output: Streptococcus faecium
Fifteen microliter binding reactions were prepared , each consisting of 20 000 c . p . m . radiolabeled DNA probe , 5 micro g of crude protein extract , non - specific competitor DNAs ( 0 . 3 micro g of yeast tRNA , 0 . 3 micro g of salmon sperm DNA and 0 . 5 micro g of E . coli DNA ) , 10 micro g of BSA , 10 mM Tris - HCl ( pH 8 . 0 ) , 10 mM MgCl2 and 8 % glycerol .
Output: E . coli
The E . coli RdgC protein is a potential negative regulator of the function of RecA ( 1 ) .
Output: E . coli
Sedimentation equilibrium data indicated that E . coli RdgC exists in solution as a mixture of oligomeric states in equilibrium , most likely monomers , dimers and tetramers ( 1 ) .
Output: E . coli
Deletion of the E . coli rdgC gene alone causes no obvious phenotype but is highly deleterious in strains lacking certain enzymes involved in recombination and replication restart ( 2 , 3 ) .
Output: E . coli
RdgC is essential for the growth of an E . coli strain lacking PriA , indicating that it might affect replication fork progression or fork rescue ( 2 ) .
Output: E . coli
However , no detectable domains or nuclease activity could be identified for the E . coli RdgC protein ( 2 , 6 ) .
Output: E . coli
The complexes of E . coli RdgC with both linear and supercoiled circular plasmid DNA were imaged using atomic force microscopy ( 7 ) .
Output: E . coli
The protein was expressed in E . coli C41 ( DE3 ) cells ( 9 ) .
Output: E . coli
This observation is consistent with the results of previous gel filtration and sedimentation equilibrium experiments , which suggested the presence of dimeric species of E . coli RdgC in solution ( 1 , 2 ) .
Output: E . coli
Electron microscopy showed that the E . coli RuvB protein , in the presence of ATP , forms a dodecamer on double - stranded DNA in which two stacked hexameric rings encircle the DNA and are oriented in opposite directions with D6 symmetry ( 33 ) .
Output: E . coli
RdgC was at its highest level during exponential phase , reaching its maximum of ~ 1000 dimers per E . coli cell .
Output: E . coli
RdgC protein was shown to have a potent effect on RecA protein - promoted DNA strand exchange in E . coli ( 1 ) .
Output: E . coli
Tetrameric species of E . coli RdgC , which started to form at around 2 micro M concentrations , were speculated to be the species with the most potent inhibitory effect , possibly by having a higher affinity for DNA ( 1 ) .
Output: E . coli
The HML and HMR loci in Saccharomyces cerevisiae are silent chromatin domains ( 1 ) .
Output: Saccharomyces cerevisiae
We performed initial cell , cytokine and complement depletion studies to investigate the possible role of these effectors in response to vaccination with heat - killed Burkholderia mallei in a susceptible BALB / c mouse model of infection .
Output: Burkholderia mallei
Burkholderia mallei , the etiologic agent of glanders , is a gram - negative , capsulated , non - motile , facultative intracelluar bacterium .
Output: Burkholderia mallei
For example , entering the gene ID 403437 results in downloading the Brca1 gene sequence for Canis familiaris .
Output: Canis familiaris
We describe a 64 - year - old man with S . faecium endocarditis in whom a six - week course of ampicillin and tobramycin , followed by additional courses of penicillin and other aminoglycosides , failed to eradicate the organism from the patient ' s mitral valve .
Output: S . faecium
This case is of interest because therapeutic failure of ampicillin and tobramycin in S . faecium endocarditis has not been reported previously , but might have been predicted on the basis of previous in vitro and in vivo studies [ 6 ] .
Output: S . faecium
Although the need for both a penicillin derivative and an aminoglycoside in the therapy of enterococcal endocarditis is widely known , it is important to distinguish between the differing efficacies of penicillin - aminoglycoside combinations for treating various species of enterococci such as S . faecium .
Output: S . faecium
Three separate morphologic colony variants were isolated from blood , each identified as S . faecium by Dr . R . R .
Output: S . faecium
S . faecium ( large colony morphotype , and poorly growing small colony morphotype ) grew from six sets of blood cultures obtained on admission .
Output: S . faecium
Bacterial and fungal stains were negative but S . faecium grew from fragments of the resected valve .
Output: S . faecium
The minimum inhibitory and bactericidal concentrations of penicillin , ampicillin , and tobramycin against the S . faecium isolated from the patient ' s blood cultures
Output: S . faecium
Organism Isolated MIC MIC MBC MIC MBC S . faecium , prior to ampicillin /
Output: S . faecium
tobramycin therapy 3 / 17 / 81 2 1 2 > 32 > 32 S . faecium , after ampicillin /
Output: S . faecium
Twenty - four hour time - kill curves for penicillin in combination with various aminoglycosides were performed by Dr . Robert Moellering on the S . faecium isolated from the patient ' s blood immediately prior to mitral valve excision and replacement ( Fig . 1 ) .
Output: S . faecium
Combinations of penicillin with kanamycin , tobramycin , sisomicin , and netilmicin have consistently failed to demonstrate synergistic killing of S . faecium in vitro [ 6 ] .
Output: S . faecium
Moellering et al . demonstrated in vivo , utilizing the rabbit model of endocarditis , that penicillin and netilmicin were not efficacious in the treatment of endocarditis caused by a low - level aminoglycoside - resistant strain of S . faecium [ 6 ] .
Output: S . faecium
In the present case , the recurrence of S . faecium endocarditis after six weeks of therapy with ampicillin and tobramycin confirms the therapeutic and clinical significance of their data , and emphasizes that tobramycin is not an aminoglycoside to be used for treatment of serious S . faecium infections .
Output: S . faecium
MBCs were only slightly higher than MICs for the three S . faecium variants obtained from our patient , and they did not appear to be tolerant strains of S . faecium .
Output: S . faecium
Lorian has also described the formation of numerous aberrant cross - walls by S . faecalis grown in the presence of subinhibitory concentrations of penicillin [ 15 ] .
Output: S . faecalis
gentamicin synergism in S . faecalis .
Output: S . faecalis
However , the lack of crossed recognition of cox2 promoters in wheat and potato is not a general situation since the A . thaliana cox2 gene is expressed and spliced when introduced into maize mitochondria , indicating that the Arabidopsis gene shares some signals with maize cox2 promoter that are sufficient for transcription ( 18 ) .
Output: A . thaliana
The sites required for transcript initiation have been recently described in A . thaliana mitochondrial genes ( 28 ) .
Output: A . thaliana
Turgor pressure measurements on leaf cells of T . voinierianum were difficult to perform because of the presence of mucilage .
Output: T . voinierianum
Experience collected with patch clamp pressure measurements on T . voinierianum and on preliminary results of other plants ( e . g . grapevines , bananas , Eucalyptus , and Arabidopsis ) showed that a smooth intercostal leaf area for clamping can readily be found .
Output: T . voinierianum
4A and B , restoration of full turgescence in T . voinierianum was faster than the preceding turgor pressure loss if the lianas were well - watered .
Output: T . voinierianum
Even though more work is required , the present study shows that the leaf patch clamp pressure probe is a promising tool to elucidate short - and long - distance water transport in T . voinierianum and other plants .
Output: T . voinierianum
Here , we have determined the crystal structure of RdgC from Pseudomonas aeruginosa .
Output: Pseudomonas aeruginosa
Pseudomonas aeruginosa is a ubiquitous environmental Gram - negative bacterium that belongs to the gamma subdivision of the Proteobacteria .
Output: Pseudomonas aeruginosa
In S . cerevisiae , there is evidence that Sir3p ( hence the SIR complex ) has much higher affinity to unacetylated histone H4 than acetylated H4 ( 5 ) .
Output: S . cerevisiae
Recently , sequences that could block the spread of silent chromatin have been discovered in S . cerevisiae ( 7 , 8 ) .
Output: S . cerevisiae
in Streptococcus Faecium Endocarditis
Output: Streptococcus Faecium
Non - commercial antibodies used were : BC2 , a mouse monoclonal to the MUC1 - EX ( gift of Dr McGuckin , Queensland University , Queensland , Australia ) , and CT2 , an Armenian hamster monoclonal to the MUC1 - CT developed in our lab [ 12 ] .
Output: Armenian hamster
Rosevalt tubers and T . aestivum var Fortal seeds were used .
Output: T . aestivum
The potato rps10 gene controlled by a T . aestivum promoter ( Figure 1A ) was transcribed as a 1204 nt precursor in wheat mitochondria , but no traces of mature RNA were observed .
Output: T . aestivum
In Arabidopsis thaliana 456 C - to - U editing events have been described ( 6 ) .
Output: Arabidopsis thaliana
To challenge the ability of mitochondrial gene expression machinery to recognize genetic information which has been lost during evolution , we decided to introduce the S . tuberosum non - cognate rps10 gene into wheat mitochondria .
Output: S . tuberosum
To test this hypothesis , it was necessary to set up the conditions for electroporation of foreign DNA into S . tuberosum mitochondria .
Output: S . tuberosum
S . tuberosum cox2 gene , including a 727 bp non - coding upstream region , was isolated by PCR from total DNA using the primer A designed from partial sequences reported by L o essl et al .
Output: S . tuberosum
The complete sequence of the S . tuberosum cox2 was determined ( accession no . DQ18064 ) .
Output: S . tuberosum
To generate the plasmid pCOXIISt containing the S . tuberosum cox2 gene , a KpnI / SpeI fragment containing the 727 bp upstream region and the complete coding region was used to replace the wheat gene from pCOXII .
Output: S . tuberosum
The coding region was isolated from total S . tuberosum DNA by PCR using primers D1 and D2 containing the restriction sites NsiI and SpeI , respectively .
Output: S . tuberosum
S . tuberosum cv .
Output: S . tuberosum
S . tuberosum rps10 transcripts are not processed in wheat mitochondria
Output: S . tuberosum
The S . tuberosum rps10 construct under control of a wheat cox2 promoter ( Figure 1A ) was incorporated into purified wheat mitochondria by electroporation as indicated in Materials and Methods .
Output: S . tuberosum
Electroporation of S . tuberosum mitochondria
Output: S . tuberosum
Since two important post - transcriptional processes , RNA editing and splicing , were inoperative when S . tuberosum rps10 was expressed in wheat mitochondria , we decided to verify whether the negative results were inherent to the rps10 chimeric constructs or due to the lack of trans - recognition elements in wheat mitochondria .
Output: S . tuberosum
For this purpose , it was necessary to set up an electroporation protocol adapted to S . tuberosum mitochondria .
Output: S . tuberosum
S . tuberosum mitochondria does not recognize the wheat cox2 promoter
Output: S . tuberosum
The construct expressing potato rps10 under the control of potato cox2 promoter was introduced into S . tuberosum isolated mitochondria .
Output: S . tuberosum
The analogous MAb and ME6b constructs , containing the S . tuberosum cox2 promoter and the C259 region inserted into rps10 exon 1 and intron were used to electroporate potato mitochondria .
Output: S . tuberosum
To best evaluate the expression of rps10 in the non - cognate mitochondria , it was necessary to set up electroporation conditions for introducing foreign DNA into S . tuberosum mitochondria .
Output: S . tuberosum
Based on the canonical structure of group II mitochondrial introns ( 21 ) , two editing sites , C2 located at the Intron Binding Site 2 ( IBS2 ) and C3 located in the intron , nine residues downstream from the end of exon 1 in S . tuberosum rps10 are of particular interest .
Output: S . tuberosum
The power function Tf = f ( Pc ) theoretically derived was experimentally confirmed by concomitant Pp and Pc measurements on intact leaflets of the liana Tetrastigma voinierianum under greenhouse conditions .
Output: Tetrastigma voinierianum
This could be verified by combined turgor pressure probe and leaf patch clamp pressure probe measurements on leaflets of the liana Tetrastigma voinierianum .
Output: Tetrastigma voinierianum
Experiments were performed on two specimens of the liana Tetrastigma voinierianum , growing in the c .
Output: Tetrastigma voinierianum
Most known members of the Burkholderiaceae are resident in the soil ; however , B . mallei is thought to be an obligate mammalian pathogen .
Output: B . mallei
Naturally acquired human infection with B . mallei , although not seen in the United States since 1945 , has occurred rarely and sporadically among laboratory workers and those in direct contact with infected animals [ 2 ] .
Output: B . mallei
Inhalation of aerosol or dust containing B . mallei can lead to septicemic , pulmonary , or chronic infections of the muscle , liver and spleen .
Output: B . mallei
Previous studies with B . mallei and the host response have shown that a mixed immune response consisting of both Th1 and Th2 - associated cytokines with a predominant IgG1 subclass does not correlate with protection [ 4 ] .
Output: B . mallei
Additional studies with passive transfer of monoclonal antibodies specific for B . mallei have correlated with early protection from infection [ 5 ] .
Output: B . mallei
Recent studies have also shown the Th1 cytokine IL - 12 to mediate partial protection to non - viable B . mallei - vaccinated mice [ 6 ] .
Output: B . mallei
Thus , full correlates of protection mediated by the adaptive immune system against B . mallei remain to be fully elucidated .
Output: B . mallei
Heat killed bacteria were used as a model of vaccination to allow evaluation of B . mallei specific immune responses .
Output: B . mallei
Heat - killed B . mallei vaccination mediates partial protection from lethal challenge
Output: B . mallei
The administration of vaccines for B . mallei during an outbreak would mandate relatively rapid onset of protection for human or veterinary use .
Output: B . mallei
Our results indicate that HK vaccination can afford partial protection to an otherwise lethal challenge of B . mallei by the i . p . route .
Output: B . mallei
To further evaluate the host TNF - alpha response during an established B . mallei chronic infection , we infected 12 BALB / c mice by the i . p . route with 1 x 106 CFU B . mallei .
Output: B . mallei
Results indicated enhanced bacterial uptake in J774A . 1 phagocytes inoculated with serum treated B . mallei ( p = . 0082 ) , compared to B . mallei alone , while heat - inactivated serum produced uptake percentages similar to those prior to serum addition ( Fig . 6 ) .
Output: B . mallei
Thus , loss or reduction of TNF - alpha and IFN - gamma levels result in significantly reduced survival rates , substantiating previous reports of the role of these factors in protection against B . mallei [ 7 ] .
Output: B . mallei
Moreover , we demonstrate a role for sustained TNF - alpha production in the maintenance of host survival throughout the course of B . mallei infection .
Output: B . mallei
Mice with an established B . mallei chronic infection rapidly lost the ability to control the growth of the bacillus upon neutralization of TNF - alpha .
Output: B . mallei
Additional studies are underway to determine more precisely the role of TNF - alpha in host protection to B . mallei .
Output: B . mallei
Multiple innate and adaptive cell types may contribute to the production of IFN - gamma in response to infection with B . mallei following vaccination .
Output: B . mallei
The effector role for IFN - gamma in mediating protection against B . mallei may include both immunoregulatory and non - regulatory functions .
Output: B . mallei
Regardless , the requirement of IFN - gamma , as demonstrated by administration of neutralizing antibody prior to infection , indicates that stimulation of IFN - gamma response is a desirable goal for a B . mallei vaccine .
Output: B . mallei
Similarly , B220 - positive cells appear to play a role in protection following vaccination with heat - killed B . mallei .
Output: B . mallei
Although CD8 - / - C57BL / 6 demonstrated no decreased survival in our HK - vaccinated model , a lack of potential endogenous protein production by HK B . mallei may have contributed to limited MHC - I presentation .
Output: B . mallei
Complement associated studies revealed increased J774A . 1 uptake of serum - treated B . mallei .
Output: B . mallei
Previous studies have demonstrated that a polysaccharide capsule is present in B . mallei , [ 14 , 15 ] although in the present study enhanced uptake with serum - treated B . mallei was observed .
Output: B . mallei
Previous reports have demonstrated the ability of B . mallei to survive within macrophage without the aid of serum coating organisms [ 16 ] .
Output: B . mallei
The current results suggest that cobra venom factor treatment may affect the modulation of the immune response to B . mallei infection through B cell activation and / or memory B cell generation .
Output: B . mallei
Understanding and defining the role of B cells in adaptive B . mallei immunity will likely be fundamental to the design of an efficacious vaccine and important goals of future research .
Output: B . mallei
B . mallei strain ATCC 23344 ( China 7 ) was cultured on Luria - Bertani agar supplemented with 4 % glycerol ( LB + 4 % G ) agar plates for 48 h at 37 degrees C .
Output: B . mallei
The absence of live B . mallei organisms in the HK preparations was confirmed after plating 10 % of the total inoculums ( v / v ) and incubating these at 37 degrees C for 48 h .
Output: B . mallei
BALB / c and C57BL / 6 mice were grouped and vaccinated with 0 . 5 mu g of HK B . mallei ( without adjuvant ) by i . p . injection using a 25 - gauge syringe .
Output: B . mallei
Two weeks post HK vaccination mice were injected i . p . with 2 x 107 CFU / 100 mu l of live B . mallei ( ~ 20 LD50 ) [ 18 ] .
Output: B . mallei
Mice , six to seven per group , were vaccinated i . p . with 1 x 105 CFU of nonviable B . mallei cell preparation in a total volume of 0 . 1 ml .
Output: B . mallei
B . mallei J774A . 1 uptake assays
Output: B . mallei
B . mallei antigen was diluted in 0 . 1 M carbonate buffer ( pH 9 . 5 ) and 50 mu l of diluted cells placed into wells .
Output: B . mallei
Gene expression studies in isolated mitochondria : Solanum tuberosum rps10 is recognized by cognate potato but not by the transcription , splicing and editing machinery of wheat mitochondria
Output: Solanum tuberosum
Of particular interest is the situation of the small ribosomal protein 10 gene ( rps10 ) , a group II intron - bearing gene which is encoded in Solanum tuberosum mitochondrial DNA but is absent from the wheat mitochondrial genome ( 20 ) .
Output: Solanum tuberosum
Here , we report the crystal structure of P . aeruginosa RdgC as determined by the multiwavelength anomalous diffraction ( MAD ) method of X - ray crystallography .
Output: P . aeruginosa
The rdgC gene ( PA3263 ) was amplified by the polymerase chain reaction ( PCR ) using the genomic DNA of P . aeruginosa strain PAO1 as a template .
Output: P . aeruginosa
The structure of P . aeruginosa RdgC was solved using two sets of MAD data collected from a crystal of the selenomethionine ( SeMet ) - substituted protein and from a mercury derivative crystal of the native protein ( Supplementary Table 1 ) .
Output: P . aeruginosa
The P . aeruginosa RdgC monomer is J - shaped and has approximate dimensions of 72 A x 60 A x 40 A ( Figure 1 ) .
Output: P . aeruginosa
When we searched for structural similarity against the Protein Data Bank database using the program DALI ( 18 ) , the P . aeruginosa RdgC monomer showed no significant similarity with a Z score above 5 .
Output: P . aeruginosa
When we elaborated the structural comparisons with individual domains of P . aeruginosa RdgC , only the center domain gave a Z score above 5 .
Output: P . aeruginosa
Using the center domain ( residues 1 - 73 and 121 - 167 ) alone , the highest structural similarity is found with the human beta 2 - adaptin appendage domain ( PDB code 1E42 , Z score = 7 . 1 , r . m . s . deviation = 2 . 5 A for 85 structurally aligned residues , residues 1 - 12 , 14 - 30 , 44 - 47 , 58 - 72 , 122 - 133 and 135 - 160 of P . aeruginosa RdgC chain A and residues 838 - 841 , 848 - 880 , 885 - 906 and 912 - 967 of the human beta 2 - adaptin appendage domain chain A ) ( 19 ) .
Output: P . aeruginosa
The next highest similarity is found with the yeast TATA - box - binding protein ( PDB code 1YTB , Z score = 5 . 7 , r . m . s . deviation = 2 . 6 A for 79 structurally aligned residues , residues 4 - 12 , 14 - 19 , 21 - 27 , 56 - 70 , 124 - 132 and 134 - 166 of P . aeruginosa RdgC chain A and residues 69 - 96 , 100 - 126 and 132 - 155 of TATA - box - binding protein , N - terminal half of chain A ) ( 20 ) .
Output: P . aeruginosa
Using the tip domain ( residues 74 - 120 ) alone , the ATPase domain of bovine Hsc70 chaperone shows highest structural similarity ( PDB code 1BA1 , Z score = 4 . 8 , r . m . s . deviation = 1 . 7 A for 45 structurally aligned residues , residues 76 - 120 of P . aeruginosa RdgC chain A and residues 230 - 253 and 256 - 276 of the Hsc70 ATPase domain ) ( 21 ) .
Output: P . aeruginosa
However , the tip domain of P . aeruginosa RdgC protein does not have such a sequence motif , and the loop in the RdgC tip domain is much more extended than that of the HhH motifs .
Output: P . aeruginosa
With the base domain ( residues 168 - 306 ) alone , the highest similarity is found with P . aeruginosa isochorismate - pyruvate lyase ( unpublished deposition ; PDB code 2H9C , Z score = 4 . 0 , r . m . s . deviation = 4 . 8 A for 51 structurally aligned residues , residues 186 - 191 , 237 - 240 , 257 - 272 and 274 - 298 of P . aeruginosa RdgC chain A and residues 36 - 41 , 50 - 53 , 55 - 66 and 68 - 96 of P . aeruginosa isochorismate - pyruvate lyase chain A ) .
Output: P . aeruginosa
The 101 - residue chain of P . aeruginosa isochorismate - pyruvate lyase is folded into three alpha - helices , two of which overlap with two alpha - helices ( alpha 2 and alpha 3 ) of the base domain of RdgC .
Output: P . aeruginosa
Our structure reveals that P . aeruginosa RdgC forms a ring - shaped dimer of approximate 2 - fold symmetry in the crystal ( Figure 1 ) .
Output: P . aeruginosa
The net charge of P . aeruginosa RdgC is highly negative , since each monomer contains 12 aspartate , 13 glutamate , 10 lysine , 9 arginine and 2 histidine residues .
Output: P . aeruginosa
ATP was not required for dsDNA binding by P . aeruginosa RdgC , either ( Supplementary Figure 2 ) .
Output: P . aeruginosa
In P . aeruginosa RdgC , the inner surface of the ring - shaped dimer is largely formed by beta - strands , while the outer surface of the ring is mostly formed by alpha - helices ( Figure 5 ) .
Output: P . aeruginosa
The predicted DNA - binding site of P . aeruginosa RdgC dimer is primarily formed by beta - strands and loops .
Output: P . aeruginosa
A similar protective role in the innate response to infection has been demonstrated for TNF - alpha in B . pseudomallei infection [ 8 ] .
Output: B . pseudomallei
The studies presented here are consistent with the essential role of these factors in the relative levels of protection conferred by vaccination with heat - killed B . pseudomallei and would appear to be viable early markers for protection from lethal acute infection [ 9 ] .
Output: B . pseudomallei
Previous studies have also demonstrated that both the humoral and cell - mediated arms are essential for protection from B . pseudomallei infection [ 10 ] .
Output: B . pseudomallei
Protection against B . pseudomallei infection by anti - LPS , capsular polysaccharide and proteins has been short - lived , suggesting that antibody production offers limited protection in the initial stages of infection by an as - yet - undefined mechanism [ 12 ] .
Output: B . pseudomallei
Complement - mediated uptake studies of B . pseudomallei by polymorphonuclear leukocytes ( PMNs ) suggest that capsule production contributes to resistance of phagocytosis by reducing C3b bacterial deposition [ 13 ] .
Output: B . pseudomallei
In organello studies have shown that S . bicolor atp6 - 1 gene was transcribed but not edited when introduced into maize mitochondria ( 18 ) .
Output: S . bicolor
AF096321 , containing the KpnI restriction sequence , and primer B derived from Triticum timopheevi cox2 ( AF336134 ) .
Output: Triticum timopheevi
In Neisseria gonorrhoeae ( the gonococcus ) , RdgC is required for efficient pilin antigenic variation and plays some role in cell growth ( 4 , 5 ) .
Output: Neisseria gonorrhoeae
The process seemed to be very similar to the refilling of the cells of the resurrection plant Myrothamnus flabellifolia with water after desiccation ( Wagner et al . , 2000 ) .
Output: Myrothamnus flabellifolia
Both lines have epithelial morphology , form tumors slowly in nude mice [ 37 ] , have mutant p53 [ 39 , 40 ] , express EGFR [ 37 ] , and lack estrogen receptor alpha [ 41 ] .
Output: nude mice
Various sequences ( designated X ) from UASs of TEF2 , AgTEF ( TEF gene from Ashbya gossypii ) and ADE2 were inserted between HML - E and HML alpha at HML Delta I ( HML locus deleted for the I silencer ) flanked by the FRT sites for the Flp1p site - specific recombinase ( Fig . 4A and B ) .
Output: Ashbya gossypii
Rap1p was expressed in Escherichia coli BL21 ( DE3 ) from pL3S5 , a pET vector carrying the RAP1 gene downstream of a T7 promoter ( 26 ) .
Output: Escherichia coli
The Escherichia coli rdgC gene encodes a DNA - binding protein of 34 kDa , whose expression level was at its highest during exponential phase , reaching its maximum at ~ 1000 dimers per cell ( 2 ) .
Output: Escherichia coli
Escherichia coli RdgC binds non - specifically to dsDNA and with higher affinity than ssDNA ( 2 ) .
Output: Escherichia coli
Bacterial tolerance has been suggested as a possible basis for therapeutic failures , particularly in the treatment of infections caused by Staphylococcus aureus with defects in the production of autolysins .
Output: Staphylococcus aureus