niryuu/sni-each-converted huggingface dataset:
Escherichia coli strains carrying recA730 ( or other recA * alleles ) exhibit dramatic increases in SOS - dependent spontaneous mutator activity .
Output: recA * alleles
Serum IgM levels showed a highly significant increase in all types of brain tumour when compared to controls .
Output: Serum IgM
TNF - alpha effect was eliminated by a 2 - bp substitution mutation in the NF - kappa B1 binding half site of the NF - kappa B cis element .
Output: TNF - alpha
However , the molecular mechanisms by which specific cis - and trans - acting factors control activity of the prodynorphin promoter are not as clearly defined .
Output: prodynorphin promoter
The nramp2 gene is comprised of 17 exons and spans more than 36 kb .
Output: nramp2 gene
Molecular analysis indicated that inactivation of H - 2L expression in nearly every null clone resulted from an apparent deletion or rearrangement of 5 ' - flanking and 5 ' - coding H - 2L sequences , with breakpoints consistently mapping to within a 550 bp GC - rich region between exon 1 and the middle of intron 2 .
Output: H - 2L
Similarly , TAM - 67 reverted the morphology of the AdoMetDC - antisense expressors .
Output: TAM - 67
In contrast , overexpression of the DREB2A cDNA induced weak expression of the target genes under unstressed conditions and caused growth retardation of the transgenic plants .
Output: DREB2A cDNA
The Brassica BTH1 gene may correspond to the Arabidopsis TH - 1 gene .
Output: Brassica BTH1 gene
The sequences of two previously known tail genes , R and S , of the temperate bacteriophage P2 and the sequence of an additional open reading frame ( orf - 30 ) located between S and V , were determined .
Output: tail genes
Inhibition of DNA topoisomerase II alpha gene expression by the p53 tumor suppressor .
Output: p53 tumor suppressor
Strains lacking a functional RPL16A gene grow as rapidly as wild type , whereas those containing a null allele of RPL16B grow more slowly than wild type .
Output: functional RPL16A gene
Treatments were two adrenocorticotrophin ( ACTH ) preparations given intramuscularly at 2 . 2 U / kg , one of the ACTH preparations given intramuscularly at 1 U / kg and a synthetic polypeptide with ACTH - like activity ( tetracosactrin , cosyntropin ) given intravenously at 5 micrograms / kg .
Output: ACTH - like
UV cross - linking and Southwestern analysis suggested that KTP1 is a DNA - binding protein clearly distinct from AP2 , and this protein may be responsible for the basal keratinocyte - specific expression of the BPAG1 gene .
Output: BPAG1 gene
A role for phosphatidylinositol 3 - kinase in the regulation of beta 1 integrin activity by the CD2 antigen .
Output: beta 1 integrin
In this respect , the promoter structure of COX genes resemble those of many house - keeping genes .
Output: COX genes
Developmentally - regulated interaction of a transcription factor complex containing CDP / cut with the early histone H3 gene promoter of the sea urchin Tetrapygus niger is associated with changes in chromatin structure and gene expression .
Output: histone H3
Using yeast two - hybrid screening , we isolated histone H3 as an interacting factor of CIA .
Output: histone H3
Human alcohol dehydrogenase ( ADH ) exists as a heterogeneous group of isozymes capable of oxidizing a wide variety of aliphatic and aromatic alcohols .
Output: Human alcohol dehydrogenase
GBF1 and GBF2 mRNA is present in light and dark grown leaves as well as in roots .
Output: GBF2 mRNA
Diagnostic importance of determining leucine aminotransferase activity in acute pancreatitis
Output: leucine aminotransferase
The activity of platelet factor 4 in plasma of healthy and high risk newborns .
Output: platelet factor 4
Interestingly , these " activation - defective " mutants segregated into two classes : 1 ) those that were unable to form dimers but that could still form higher order oligomers and transform cells , and 2 ) those that were defective for PDGF - R binding and were transformation - incompetent .
Output: PDGF - R
This mutant also functions in vivo as a trans - acting dominant negative regulator : the transcriptional inducibility of the HIV long terminal repeat ( which contains two potential NF - kappa B binding sites ) by phorbol ester ( PMA ) is inhibited when it is co - transfected into CD4 + T cells with the delta SP mutant .
Output: CD4 +
Expression of FNCAT increased on serum treatment indicating that the region of the FN gene between positions + 69 and - 510 bp mediated serum responsiveness .
Output: FN gene
For Thermus strain ZO5 dihydroorotase ( DHOase ; encoded by pyrC ) , the highest similarity scores ( about 40 % identity ) were obtained with DHOases from B . caldolyticus and Bacillus subtilis .
Output: ZO5 dihydroorotase
Rat cholesterol side - chain cleavage cytochrome P - 450 ( P - 450scc ) gene .
Output: P - 450scc
A phase II clinical trial on MDS was conducted in a cooperative study with orally administrable ara - C analogue , PLAC , which is resistant to cytidine deaminase and had shown an anti - tumor activity on various experimental tumors by oral route .
Output: cytidine deaminase
No effect of 4 beta - phorbol 12 - myristate 13 - acetate was observed on the expression of hnRNP H .
Output: hnRNP H
These findings suggest that NMDA receptor blockade may ameliorate the dyskinetic complications of long - term levodopa therapy , without diminishing the beneficial effects on parkinsonian signs .
Output: NMDA receptor
It is concluded that the new class of competitive NMDA receptor antagonists , exemplified by CGP 37849 , is the most promising candidate for clinical trials in anxiety disorders .
Output: NMDA receptor
Deletion analysis of the 7 . 3 - kb GATA - 2 promoter region revealed that a 1 . 1 - kb DNA sequence is critical for expression of GATA - 2 in neurons .
Output: GATA - 2
However , at the time of salvage treatment , the mean serum PSA levels were 9 . 1 and 1 . 1 ng / mL for the salvage RP and salvage RT groups , respectively ( P = 0 . 0001 ) .
Output: serum PSA
Regulation of the human stress response gene GADD153 expression : role of ETS1 and FLI - 1 gene products .
Output: FLI - 1
Tumor necrosis factor levels were measured by enzyme - linked immunoabsorbent assay in plasma samples obtained from the hyperthermic isolated limb perfusion circuit and systemic circulation .
Output: Tumor necrosis factor
The STP1 locus is located on chromosome IV close to at least two other genes involved in RNA splicing : PRP3 and SPP41 .
Output: STP1 locus
Also , anti - HIV gp120 and anti - CD4 crosslinking induced a 10 - 15 - fold increase in levels of both PI 3 - and PI 4 - kinase activity in anti - CD4 precipitates .
Output: anti - CD4
After dialysis online , lactate was converted by means of lactate oxidase immobilized to porous glass , and the depletion of oxygen was registered by means of a Clark electrode .
Output: lactate oxidase
We previously described the upregulation of the MT2 antigen during urodele limb regeneration and characterized the MT2 antigen as a 310 - to 325 - kDa chondroitin - sulfated glycoprotein with a core protein of 285 - 300 kDa .
Output: MT2 antigen
The nucleotide sequence of a PCR - amplified SMT3A genomic DNA fragment was found to be identical to that of SMT3A cDNA , indicating the absence of intron ( s ) in its protein coding region .
Output: SMT3A cDNA
Uncertainty exists about the function of these potential isoforms of the Cbfa1 gene .
Output: Cbfa1 gene
Liver disease and HCV infection after transplantation of organs from hepatitis C antibody positive donors .
Output: hepatitis C antibody
Human epidermal growth factor ( EGF ) , a naturally occurring protein , has been implicated in the protection of gastrointestinal mucosal integrity .
Output: epidermal growth factor
Furthermore , it was found that the Shb - overexpressing cells extended neurites in response to epidermal growth factor .
Output: epidermal growth factor
The elevated levels of inositol phosphates resulting from N ( epi ) alpha qQ209L expression were similar to those obtained with carbachol activation of the M1 muscarinic acetylcholine receptor .
Output: acetylcholine receptor
Penicillamine - induced myasthenia gravis associated with antibodies to acetylcholine receptor .
Output: acetylcholine receptor
These data suggest that weight gain or increased caloric intake , in contrast to its large effect on peripheral thyroid function , has relatively little effect on CNS TRH activity .
Output: CNS TRH
The cDNA contains a reading frame for a 145 - amino - acid protein and it lacks the UGA codons , which have been found in the reading frame of the mouse MCS cDNA and have been presumed to encode the selenocysteine in the amino terminal of the deduced mouse amino acid sequence .
Output: mouse MCS cDNA
In addition , there was an increase in the amount of p120 Ras - specific GTPase - activating protein ( GAP ) and GAP - associated p190 .
Output: p120 Ras
This distinct biochemical difference between STAT5A and STAT5B was confirmed with purified activated STAT5 recombinant proteins .
Output: STAT5 recombinant proteins
Nuclear transcription assays confirmed that cys - 3 + was under sulfur - regulated transcriptional control and that cys - 3 + transcription was constitutive in sulfur controller ( scon ) - negative regulator mutants .
Output: sulfur controller
Many human malignant cells lack methylthioadenosine phosphorylase ( MTAP ) enzyme activity .
Output: methylthioadenosine phosphorylase
Before and 6 and 12 months after H . pylori eradication the patients were evaluated for fasting gastrinemia and pepsinogen I , basal and peak acid output , and detailed histological assessment including the ECL cell proliferative patterns .
Output: pepsinogen I
Cotransfection of pINV - 2473 , a construct containing 2473 base pairs of hINV upstream sequence linked to luciferase , with POU homeodomain transcription factors Oct1 , Oct2 , Brn4 , SCIP , Skn1a or Skn1i , results in a strong suppression of basal promoter activity .
Output: hINV upstream sequence
Having demonstrated proper localization of GFP - calmodulin in budding yeast , we examined the localization of a fusion between GFP and calmodulin ( GFP - Camlp ) in fission yeast , where calmodulin had not been localized by any method .
Output: GFP - Camlp
Thus the Hi - RARE represents a new type of RA response element with a role in the modulation of the expression of MHC class 1 family genes .
Output: MHC class 1
Serum estriol levels during beta - receptor agonist infusion in the third trimester of pregnancy .
Output: beta - receptor
Sequence analysis and electrophoretic mobility shift experiments suggest that GnSE response elements interact , in these two regions , with GATA - and LIM - related factors , respectively .
Output: GnSE response elements
A close homologue of the APK2a gene , named APK2b , was also isolated from the Arabidopsis cDNA library .
Output: APK2a gene
We prospectively studied serum prolactin ( PRL ) elevation after different types of documented seizures in 17 patients .
Output: serum prolactin
Effect of bromocriptine and metoclopramide on serum prolactin levels in patients with amyotrophic lateral sclerosis .
Output: serum prolactin
The clr1 locus regulates the expression of the cryptic mating - type loci of fission yeast .
Output: clr1 locus
Ptx1 belongs to an expanding family of bicoid - related vertebrate homeobox genes .
Output: bicoid - related
In F9 , which is a prototype of embryonal carcinoma cells expressing hst , the expression of hst gene is positively regulated by a downstream octamer motif that functions as an enhancer .
Output: hst gene
The feasibility of creating CR1 / Ig chimeras makes possible a new strategy of targeting complement inhibition by the use of Ig fusion partners having particular antigenic specificities .
Output: Ig fusion partners
3 - 5 - fold increases in EGF receptor but not p185 ( neu ) tyrosine phosphorylation occur following Gi - coupled receptor stimulation .
Output: EGF receptor
We have shown previously that EGF receptor activation stimulates gastrin gene expression through a GC - rich element called gERE .
Output: EGF receptor
We examined the mechanisms by which two different types of photonic radiation , short wavelength UV ( UV - C ) and gamma radiation , activate transcription factor NF - kappaB .
Output: NF - kappaB
Conversion of an NF - kappaB into a GABP binding site is likely to have occurred also during the worldwide spread of HIV - 1 , as we noticed the same LTR modification in subtype E isolates from Thailand .
Output: NF - kappaB
Papillomavirus type 16 oncogenes downregulate expression of interferon - responsive genes and upregulate proliferation - associated and NF - kappaB - responsive genes in cervical keratinocytes .
Output: NF - kappaB
NF - kappaB only partially mediates Epstein - Barr virus latent membrane protein 1 activation of B cells .
Output: NF - kappaB
The activation of NF - kappaB - dependent reporter gene transcription by E1A was potently suppressed upon coexpression of the E1B 19 - kDa protein ( 19K ) .
Output: NF - kappaB
These results provide strong evidence that conserved interhelical packing interactions in the gp41 core are important determinants of HIV - 1 entry and its inhibition .
Output: gp41 core
Deletion mutants of the human CHOP promoter identify a region comprising nucleotides - 75 to - 104 required for both constitutive and ER - stress - inducible expression .
Output: human CHOP promoter
Both viruses express the mil / raf oncogene product as a gag - fusion polyprotein , while the myc oncogene of MH2 is expressed via a subgenomic mRNA .
Output: gag - fusion
Extracellular protein kinase A as a cancer biomarker : its expression by tumor cells and reversal by a myristate - lacking Calpha and RIIbeta subunit overexpression .
Output: protein kinase A
In isolated olfactory cilia certain odorants elicit a rapid and transient cAMP response , terminated by a concerted process which requires the action of two protein kinases , protein kinase A ( PKA ) and a receptor - specific kinase ( GRK3 ) ( Schleicher , S . , Boekhoff , I .
Output: protein kinase A
These proteins were detected by an antibody which recognizes the N - terminus of SRK3 and , in an F2 progeny segregating for the S3 haplotype , were only expressed in plants possessing the S3 haplotype .
Output: S3 haplotype
We present here the complete primary structure of human gp330 , the human variant of the principal kidney autoantigen causing Heymann membranous glomerulonephritis in rats .
Output: human gp330
By contrast , CIS failed to affect the IL - 9 response .
Output: IL - 9
The human IFI16 gene is a member of an interferon - inducible family of mouse and human genes closely linked on syntenic regions of chromosome 1 .
Output: human IFI16 gene
HNF4alpha7 mRNA is also found in totipotent embryonic stem cells .
Output: HNF4alpha7 mRNA
We suggest that the responses to types I and II collagen were caused by a cross - reaction with type III collagen peptides .
Output: type III collagen
TAF - 1 activates transcription constitutively and was mapped to the first 32 amino acids of the A - region .
Output: TAF - 1
Genomic organization of the human multidrug resistance ( MDR1 ) gene and origin of P - glycoproteins .
Output: P - glycoproteins
The purified RAG1 protein overexpressed in E . coli exhibited the expected cleavage activity when combined with RAG2 purified from transfected 293T cells .
Output: RAG1 protein
Analysis of exon 2 of the factor IX gene revealed a C - - > T mutation in codon 10 of the propeptide region , resulting in the substitution of alanine by valine .
Output: factor IX gene
These results support an in vivo interaction between Nck and CKI - gamma2 and suggest that CKI - gamma2 could be involved in signaling pathways downstream of RTKs .
Output: CKI - gamma2
The Rep78 protein of adeno - associated virus ( AAV ) contains amino acid sequence motifs common to rolling - circle replication ( RCR ) initiator proteins .
Output: Rep78 protein
The gamma - GCS gene is expressed ubiquitously and induced coordinately with NAD ( P ) H : quinone oxidoreductase ( 1 ) ( NQO1 ) and glutathione S - transferase Ya ( GST Ya ) in response to xenobiotics and antioxidants .
Output: GST Ya
Hence the GAP1 gene encodes a protein with characteristics typical of integral membrane proteins translocating ligants across cellular membranes .
Output: GAP1 gene
Its activity was twice that of a construct where the CAT gene was driven by the H - 2Kb 5 ' enhancer region ( H2TF1 / KBF1 site ) and comparable to that of pRSVCAT construct carrying the strong Rous sarcoma virus LTR enhancer .
Output: CAT gene
To probe the inter - relationship between the virion - anchoring function and the oligomerization function , we constructed two serotype 3 ( T3 ) sigma 1 deletion mutants in SV40 expression vectors , one lacking the hydrophobic tail and the hinge , and the other lacking an adjacent region which constituted part of the coiled - coil .
Output: sigma 1
The fru gene has seven exons spanning 15 - kb encoding two transcripts with ORFs of 841 and 695 amino acids .
Output: fru gene
A survey of children with HBsAg markers related to their parents HBV markers .
Output: HBsAg markers
Gene expression is in part controlled by chromatin remodeling factors and the acetylation state of nucleosomal histones .
Output: nucleosomal histones
We suggest that this effect of Myc is mediated by its action upstream of cyclin E - CDK2 , and occurs via the neutralization of p27 ( Kip1 ) family proteins , rather than induction of Cdc25A .
Output: cyclin E
Adenovirus - mediated gene transfer of MMAC1 / PTEN to glioblastoma cells inhibits S phase entry by the recruitment of p27Kip1 into cyclin E / CDK2 complexes .
Output: cyclin E
These three phases of cyclin E association with chromatin may facilitate the diverse activities of cyclin E - - Cdk2 in initiating replication , blocking rereplication , and allowing resetting of origins after mitosis .
Output: cyclin E
Most significantly , the cyclin E - Cdk2 complex is maximally active at the G1 / S transition , and overexpression of cyclin E decreases the time it takes the cell to complete G1 and enter S phase .
Output: cyclin E
When a longer - 714 to + 78 fragment of the PBGD promoter is used , the - 70 GATA mutant still displays erythroid - specific activity and is cis - activated by the 5 ' HS - 2 enhancer , while the - 100 CCACC mutant is completely inactive in the absence or in the presence of the 5 ' HS - 2 enhancer .
Output: PBGD promoter
In one trial , 116 subjects with transfusion - related chronic hepatitis C were treated with lymphoblastoid interferon ( 5 MU / m2 three times a week for 2 mo , then 3 MU / m2 three times a week for 4 or 10 mo ) .
Output: lymphoblastoid interferon
However , electron microscopy revealed that while sec4 mutant cells accumulate secretory vesicles , ypt31 / 32 mutant cells accumulate aberrant Golgi structures .
Output: sec4 mutant
We analyzed the effects of light on tubulin mRNA abundance in Arabidopsis seedlings using RNA gel blot hybridizations and gene - specific probes .
Output: tubulin mRNA
In turn , production of sufficient amounts of TraR apparently is sensitive to a cellular function requiring RNase D .
Output: RNase D
13 : 961 - 969 , 1993 ) suggested that T antigen could mediate transcriptional activation through interaction with the TATA - binding protein , as well as upstream bound transcription factors .
Output: T antigen
The gp41 peptide ( Glu - Leu - Asp - Lys - Trp - Ala ) fused to the C - terminus of Sj GST forms a loop stabilized by symmetry - related GSTs .
Output: Sj GST
As regards the short stature , they have proved that the syndrome is related to low levels of somatomedin C ( SmC ) .
Output: somatomedin C
Here we demonstrate that CD40 or IgM receptor stimulation of primary B cells results in transactivation of this enhancer .
Output: IgM receptor
We have previously demonstrated that pyrene in diesel - exhaust particles ( DEP ) has an adjuvant activity on immunoglobulin E ( IgE ) antibody production in mice immunized with Japanese cedar pollen allergen ( JCPA ) or ovalbumin ( OA ) intraperitoneally .
Output: immunoglobulin E
Both RNA14 and RNA15 wild - type genes , when cloned on a multicopy plasmid , are able to suppress the temperature - sensitive phenotype of strains bearing either the rna14 or the rna15 mutation , suggesting that the encoded proteins could interact with each other .
Output: rna15 mutation
Sorting information to the chloroplastic inner envelope is contained in an NH2 - proximal part of mature IEP110 ( 110N ) .
Output: mature IEP110
The requirement for an essential interaction between NPH I and H4L provides an explanation for the observed restriction of transcription termination to early viral genes .
Output: NPH I
Using reporter gene constructs driven by the CD4 promoter , we report that HHV - 6 can efficiently transactivate such genetic elements .
Output: CD4 promoter
Free protein S deficiency in acute ischemic stroke .
Output: protein S
Our data define Ile ( 16 ) , Val ( 18 ) , Val ( 31 ) , and Ile ( 33 ) as crucial for protein S binding , with secondary effects from Leu ( 38 ) and Val ( 39 ) .
Output: protein S
This activity creates a 4 - bp staggered cut with 3 ' OH overhangs within a specific sequence of the 21S rRNA gene of omega - strains .
Output: 21S rRNA gene
Although analysis of the similarity between the 44 - kDa protein and the E . coli RecA protein did not show any significant homology between them , it revealed their identity by five amino - acid residues involved in the formation of the epitope that recognized the paratope of the RecA protein antibody for subsequent epitope - paratope binding of these proteins .
Output: RecA protein antibody
Following EGF or NGF stimulation of the v - CrkPC12 cells , the v - Crk protein itself became tyrosine phosphorylated within 1 min .
Output: v - Crk
Most interestingly , although the effector plasmid containing the ICP27 gene had little effect on its own , two different and marked effects depending on the target were observed when ICP27 was combined with ICP4 or ICP0 or both .
Output: ICP27 gene
The hyperpigmentation is linked to the presence of numerous single melanosomes and polymelanosomes in keratinocytes at all levels of the epidermis , and in dermal Factor XIIIa - positive dendrocytes .
Output: Factor XIIIa
A p53 cDNA deletion mutant ( delta pro AE ) , which lacks this entire proline - rich domain ( deleted for amino acids 62 - 91 ) , was created and characterized for a variety of p53 functions .
Output: p53 cDNA
The goals of these experiments were to determine whether lactational anestrus would be prolonged by a 48 - h fast at days 13 and 14 postpartum ( pp ) and , if so , to determine whether this effect could be reversed by treatment with the Ob protein leptin .
Output: Ob protein
These results demonstrate that the entire E3L gene is required for pathogenesis in the mouse model .
Output: E3L gene
Second , overexpression of RIM11 can suppress an ime1 missense mutation ( ime1 - L321F ) but not an ime1 deletion .
Output: ime1 - L321F
These larger sizes , however , are consistent with predictions from the DNA sequence of the pol gene .
Output: pol gene
We have isolated a unique murine FLT3 cDNA that codes for a variant isoform of FLT3 , devoid of the fifth Ig - like domain , by comparison with the prototypic form .
Output: murine FLT3 cDNA
Identification of a putative chromosomal replication origin from Helicobacter pylori and its interaction with the initiator protein DnaA .
Output: initiator protein DnaA
Dual signal peptides mediate the signal recognition particle / Sec - independent insertion of a thylakoid membrane polyprotein , PsbY .
Output: signal recognition particle
In vitro DNA binding assays indicate that the elements identified can specifically interact with c - Ets - 1 protein .
Output: Ets - 1
Surprisingly , although Mist1 binds to E - boxes in vivo , the Mist1 protein lacks a functional transcription activation domain .
Output: Mist1 protein
Rat p8 mRNA was discovered because of its strong activation in pancreas during the acute phase of pancreatitis .
Output: Rat p8 mRNA
Nuclear localization of this SNF2 - like putative helicase is dependent on a nuclear localization sequence located in the NH2 - terminal region .
Output: SNF2 - like
These results extend our knowledge of this SNF2 - like family member and suggest a role for PASG in leukemogenesis .
Output: SNF2 - like
Steady - state levels of murine MMR mRNA were measured in the macrophage cell line J774E , which is known to express the protein at the cell surface .
Output: murine MMR mRNA
Mutation of K14 in H3 , which serves as the major target of recombinant Gcn5p acetylation in vitro , confers a strong , synthetic growth defect in gcn5 cells .
Output: recombinant Gcn5p
Mutational analysis of the IME2 UAS reveals two critical sequence elements : a G + C - rich sequence ( called URS1 ) , previously identified at many meiotic genes , and a newly described element , the T4C site , that we found at a subset of meiotic genes .
Output: IME2 UAS
To determine the role of the transmembrane region of HN on fusion - promoting activity , mutant HN proteins were expressed and their biological activities examined .
Output: mutant HN proteins
Soluble recombinant RI and soluble recombinant C alpha can associate in vitro and be activated by cyclic AMP .
Output: C alpha
[ ( OP ) 2Cu ] + also detected protections in the C alpha - helix , the interdomain hinge , and beta - strands 2 - 7 .
Output: C alpha
Critical review of tests for the HBs antigen in the detection of infectious blood
Output: HBs antigen
Overexpression of wild type and SeCys / Cys mutant of human thioredoxin reductase in E . coli : the role of selenocysteine in the catalytic activity .
Output: human thioredoxin reductase
Legionella antibodies in domestic animals
Output: Legionella antibodies
Despite a lowering of her plasma ACTH concentration during therapy with valproic acid , the patient ' s tumour showed no evidence of regression while she was taking the drug .
Output: plasma ACTH
Experimental studies and clinical application of plasma ACTH radioimmunoassay kit without extraction process
Output: plasma ACTH
Peak plasma ACTH responses were not significantly different between control and patient groups .
Output: plasma ACTH
Biochemical studies have identified a cellular non - P element - encoded DNA binding protein , termed the inverted repeat binding protein ( IRBP ) , that specifically interacts with the outer half of the 31 - bp terminal inverted repeats .
Output: P element
Lastly , several restriction length polymorphisms were identified and mapped within a 1 kb region located immediately upstream from the JH cluster .
Output: JH cluster
The uap100 cis - acting , up - promoter , constitutive mutation is a duplication that comprises two GATA sites and suppresses weakly the AREA zinc finger mutation but does not alleviate the need for functional UAY and AREA proteins .
Output: AREA proteins
After 5 - h thermal induction of cells carrying the runaway recombinant pBS1 , protein B2 constituted 40 % of the soluble protein fraction of the cells .
Output: protein B2
The Valpha and Vbeta domains have the canonical features of known teleost and mammalian TCR V domains , including conserved residues in the beginning of FR2 and at the end of FR3 .
Output: Vbeta domains
The corresponding tetrapeptide sequences SSPD and SATD for human and mouse PKC - epsilon , respectively , are unusual for caspase - 3 .
Output: caspase - 3
These results induce that the putative TATA box and initiator are not involved in the promoter activity , and that the vitronectin promoter lacks the TATA box , initiator and GC box .
Output: vitronectin promoter
GH signals by interacting with GH receptor ( GHR ) .
Output: GH receptor
Therefore the effect of GAL11 on PGK transcription must be mediated at the PGK UAS , presumably as part of the activation complex .
Output: PGK UAS
The P - ITIM - compelled multi - phosphoprotein complex binds to and activates SHP - 2 , which in turn dephosphorylates SHIP and Shc and probably other substrates .
Output: SHP - 2
No significant difference was seen found between the two groups in the degree of postoperative deterioration in cardiopulmonary function or in interleukin - 6 levels .
Output: interleukin - 6
Co - expression of PLD1 in COS - 7 cells with the two recombinant CK2 subunits , alpha or beta , suggests that the association of PLD1 with the kinase is through the beta subunit .
Output: recombinant CK2 subunits
Low serum C3 values were observed in all 11 children at some stage of their illness .
Output: serum C3
Symptoms , skin - prick tests ( SPT ) with environmental allergens and Pt salt , total serum IgE , lung function , and bronchial hyperresponsiveness were assessed by standard procedures .
Output: serum IgE
Patients with the chronic photosensitivity dermatitis with actinic reticuloid syndrome have high total serum IgE concentrations .
Output: serum IgE
To further study the role of tTG in liver disease , we initiated investigations into the effect of a proinflammatory mediator , tumor necrosis factor ( TNF ) - alpha , on tTG activity in cultured liver cells .
Output: tumor necrosis factor
The polypeptide encoded by the cDNA contained 450 amino acids with a calculated M ( r ) of 52 , 057 , and showed significant homology with human and mouse CAD ( 22 % identity ) .
Output: mouse CAD
The SEC27 gene was cloned , and the sequence predicts a 99 . 4 - kDa protein with 45 % sequence identity to mammalian beta ' - COP .
Output: SEC27 gene
In contrast , dominant negative Rac ( N17Rac1 ) inhibited JNK activation by Galpha12 in HEK293 cells as well as three other cell lines .
Output: dominant negative Rac
This demonstrates the presence of a PKC - dependent pathway which functions independently from Lck in MAP kinase activation .
Output: MAP kinase
These observations demonstrate that KSR is capable of uncoupling the MAP kinase activation from its target phosphorylation , and thus provide a novel mechanism for modulating the Ras - MAP kinase signaling pathway .
Output: MAP kinase
Furthermore , CL100 suppresses the [ val12 ] ras - induced activation of MAP kinase in a cell - free system from Xenopus oocytes .
Output: MAP kinase
However , microinjection of reconstituted mRNPs into Xenopus oocytes demonstrates that although translational repression occurs in the absence of consensus RNA binding sequences for FRGY2 , the presence of FRGY2 recognition elements within mRNA potentiates translational repression .
Output: FRGY2 recognition elements
Effects of timepidium bromide ( TB ; anticholinergic agent ) , acetylcholine ( ACh ) and neostigmine ( Neost ) on gastric and duodenal blood flow distribution were studied by the use of 131I - labeled macroaggregated human serum albumin ( MAA ) in rabbits .
Output: serum albumin
Recombinant caspases are typically produced in Escherichia coli expression systems with the attendant problems of solubilization , re - folding and activation of the protease .
Output: Recombinant caspases
PC12 cultures that had been " primed " in this way showed an accelerated and augmented differentiation response to nerve growth factor .
Output: nerve growth factor
The best - studied neurotrophin , nerve growth factor ( NGF ) , is a major survival factor in sympathetic and sensory neurons and promotes differentiation in a well - studied model system , PC12 cells .
Output: nerve growth factor
The yeast genome contains a single ORD1 gene that resides on chromosome XI .
Output: ORD1 gene
When targeted to the GAL1 promoter by fusing with the Gal4p DNA - binding domain , Sko1p acts as an Ssn6 / Tup1p - dependent repressor regulated by osmotic stress .
Output: GAL1 promoter
An X - linked zinc finger gene mapping to Xq21 . 1 - q21 . 3 closely related to ZFX and ZFY : possible origins from a common ancestral gene .
Output: zinc finger gene
The myeloid zinc finger gene 1 ( MZF1 ) encodes a C ( 2 ) H ( 2 ) zinc finger transcription factor that regulates granulopoiesis and may have a regulatory role in cellular proliferation and oncogenesis .
Output: zinc finger gene
In addition , wild - type and mutated DNA templates were used as probes in DNase I protection experiments to determine sites of protein - DNA interaction .
Output: DNase I
DNase I protection analysis as well as oligonucleotide competition experiments indicate that this binding is sequence specific .
Output: DNase I
Moreover , such mutations lead to a dramatic transition in chromatin structure : the DNase I hypersensitive region is replaced by two stable , sequence - positioned nucleosomes .
Output: DNase I
The human SHBG proximal promoter was analyzed by DNase I footprinting , and the functional significance of 6 footprinted regions ( FP1 - FP6 ) within the proximal promoter was studied in human HepG2 hepatoblastoma cells .
Output: DNase I
In this work , we determined the sequences of eight VirF - binding sites from four different genes , by DNase I or hydroxyl radical footprinting .
Output: DNase I
A 360 - bp DNA fragment located over 500 bp upstream from the cfl transcriptional start site was used in DNase I protection assays to define the specific bases bound by CorR .
Output: DNase I
Nonlethal sec71 - 1 and sec72 - 1 mutations eliminate proteins associated with the Sec63p - BiP complex from S . cerevisiae .
Output: sec71 - 1
On the other hand , NE transport and antagonist ( [ 125I ] RTI - 55 ) binding assays on whole LLC - NET cells treated with tunicamycin reveal a pronounced reduction in NE transport activity and hNET membrane density paralleled by an inability of NET proteins to replenish the higher M ( r ) hNET pool .
Output: NET proteins
The structure of the murine Dtk gene has been determined .
Output: murine Dtk gene
The distinct spatial pattern of expression , and unusual amino acid sequence in its DNA binding domain , may indicate a particular role for DTEF - 1 in cell - specific gene regulation .
Output: DTEF - 1
Long - range mapping by pulse - field gel electrophoresis indicated that the three mdr genes are closely linked on a genomic DNA segment of approximately 625 kilobases .
Output: mdr genes
Multiple aortic thrombi associated with protein C and S deficiency .
Output: protein C
The adoption of a twisted structure of importin - beta is essential for the protein - protein interaction required for nuclear transport .
Output: importin - beta
Antibodies specific to the different members of the Jun and Fos family of transcription factors show that , in gel retardation assays , a Jun - like factor is a component of the H3 . 2 specific complex .
Output: Fos family
The D443N substitution is an activating mutation that increases the activity of SCAP and renders it resistant to inhibition by 25 - hydroxycholesterol .
Output: D443N substitution
We have utilized transient transfections , mutation analysis , electromobility gel - shifts , and immunoblot analysis to test the hypothesis that expression of the CTalpha gene is controlled in part by the binding of three trans - acting nuclear factors , Sp1 , Sp2 , and Sp3 .
Output: CTalpha gene
PtdIns ( 4 , 5 ) P2 synthesis , on the other hand , is only moderately affected even in fab1Delta mutants .
Output: fab1Delta mutants
A 4 . 0 - kb Nlk message is also present during embryogenesis , detectable at day E10 .
Output: Nlk message
The atopic disposition , indicated by positive skin reactions and IgE antibody titers etc . , and the bronchial reactivity to inhaled acetylcholine were examined on the following three groups : ( 1 ) 20 young adults with a history of childhood asthma who have been symptom - free for more than 4 yr ; ( 2 ) 20 current asthmatics , and ( 3 ) 20 healthy young adults .
Output: IgE antibody
IgE antibody to twelve common food and inhalant allergens was measured by enzyme - linked immunosorbent assay ( ELISA ) in the sera of thirteen atopic patients with one or more allergic disorders ( asthma occurring in eleven ; rhinitis in ten ; eczema in six ; urticaria in four ; mouth and gastro - intestinal symptoms in six ) , of twelve non - atopic patients with various clinical symptoms ( asthma in four ; rhinitis in four ; eczema in one ; urticaria in two ; mouth and gastro - intestinal symptoms in four ) and sixteen cord blood sera .
Output: IgE antibody
Uncoupling gene activity from chromatin structure : promoter mutations can inactivate transcription of the yeast HSP82 gene without eliminating nucleosome - free regions .
Output: yeast HSP82 gene
The PK domain showed pairwise in vitro binding interactions with the pseudokinase , HisRS , and C - term domains ; additionally , the HisRS domain interacted with the C - term region .
Output: HisRS domain
A conventional N - terminal signal sequence was not detected in the NodO protein .
Output: NodO protein
This study was undertaken to evaluate the physical and biological characteristics of nebulized interleukin 2 liposomes .
Output: interleukin 2
Promoter activity of the proliferating - cell nuclear antigen gene is associated with inducible CRE - binding proteins in interleukin 2 - stimulated T lymphocytes .
Output: interleukin 2
We were unable to construct chvI or chvG insertion mutants of R . meliloti , whereas mutants carrying insertions outside of these genes were readily obtained .
Output: chvG insertion mutants
Plasma LH and progesterone levels after single or multiple injections of synthetic LH - RH in anoestrous ewes and comparison with levels during the oestrous cycle .
Output: Plasma LH
First , a GATA - 1 motif was found to bind nuclear factor ( s ) , presumably the GATA - 1 factor , present in K - 562 cell extracts and in living K - 562 cells .
Output: GATA - 1
ES cells in which the gene for the erythroid transcription factor GATA - 1 has been disrupted fail to produce mature erythroid cells either in vivo or in vitro .
Output: GATA - 1
We have now isolated cDNA for an invertebrate Pax - 6 protein from sea urchin embryos .
Output: Pax - 6
Pax - 6 constructs lacking the C - terminal activation domain repressed betaB1 - crystallin promoter activity as effectively as the full - length protein , but the PD alone or Pax - 6 ( 5a ) , a splice variant with an altered PD affecting its DNA binding specificity , did not .
Output: Pax - 6
Several reports assert that prolactin affects the delta 5 and delta 4 pathways through its effect on the activity of 3beta - hydroxysteroid dehydrogenase ( 3beta - OHSD ) .
Output: 3beta - OHSD
Located between the 3 ' trans - spliced leader acceptor site and the translational initiation codon of the GP63 gene is an area of conserved hexanucleotide direct repeats ( CTCGCC ) which vary in number according to species .
Output: GP63 gene
Platelet factor 4 levels in patients with coronary artery disease .
Output: Platelet factor 4
Electron microscopic visualization of the general population of active genes in flies overexpressing hnRNP proteins also indicated that the great majority of genes seemed normal in terms of cotranscriptional RNA processing events , although there were a few abnormalities consistent with rare exon - skipping events .
Output: hnRNP proteins
These data show that tyrosine phosphorylation by FES affects the interaction of BCR with multiple signaling partners and suggest a general role for BCR in non - receptor protein - tyrosine kinase regulation and signal transduction .
Output: tyrosine kinase
The model of the complex provides a rationale for conserved ITAM residues , substrate specificity , and suggests that substrate binds only the active conformation of the Src family tyrosine kinase , unlike the ATP cofactor , which can bind the inactive form .
Output: tyrosine kinase
For the P transcript from phage with the G ( - ) orientation , full termination activity required both the region containing the stem - loop structure and upstream sequences .
Output: P transcript
The STS sequence WI - 14920 is in fact derived from the 3 ' - untranslated region of the human PUNC gene .
Output: human PUNC gene
There are two regulatory regions between the US3 and the US6 transcription units .
Output: US6 transcription units
In the virus - infected cells , translation of the M1 protein was reduced to 10 to 20 % of that of the wild - type virus ; however , the translation of neither the nucleoprotein nor NS1 was significantly interfered with , indicating the important role of NS1 in translational stimulation of the M1 protein .
Output: M1 protein
The association of I - 92 with p92 , p84 , p75 , p73 , p69 , and p57 was completely reversible after treatment with the detergent deoxycholate ( DOC ) .
Output: I - 92
Further , we find that the BMI1 protein can also interact with itself .
Output: BMI1 protein
To obtain information on the functional role of the Rac1 / p38 / MAPKAPK - 2 pathway in RA signaling , the effects of pharmacological inhibition of p38 on RA - induced gene transcription and cell differentiation were determined .
Output: MAPKAPK - 2
Both PMP20 gene products contain the carboxyl - terminal sequence AKL , similar to the putative SKL peroxisomal sorting sequence ( Gould , S .
Output: PMP20 gene products
The effect of the NMDA - receptor antagonist ( + / - ) - CPP on the conditioned - reflex activation of an operant reaction in the brain electrical self - stimulation test in rats
Output: NMDA - receptor
Evidence that the AT1A receptor activates transcription factor - Stat91 and / or a related protein .
Output: AT1A receptor
These data suggest that reduction in PRA may have contributed to the hemodynamic effects of this peak III PDE inhibitor .
Output: peak III PDE
Profilins IIa and IIb are also present in humans , suggesting that all mammals have three profilin isoforms .
Output: profilin isoforms
We have produced the Ure2 protein to high yield in Escherichia coli from a synthetic gene .
Output: Ure2 protein
Injection of horseradish peroxidase ( HRP ) into the cerebellar flocculus of the rat was employed to identify neurons in the abducens nucleus that project to the flocculus .
Output: horseradish peroxidase
The electron spin resonance study also demonstrated that the formation of superoxide - DMPO spin adduct was strongly inhibited by a selective cyclooxygenase - 2 inhibitor , etodolac , in a concentration - dependent manner .
Output: cyclooxygenase - 2
BACKGROUND : Murine Nramp is a candidate for the macrophage resistance gene Ity / Lsh / Bcg .
Output: Murine Nramp
Plasma prolactin concentrations in pinealectomized ewes receiving melatonin treatment and in pineal intact ewes maintained under a non - 24 - hour photoperiod .
Output: Plasma prolactin
Plasma prolactin in patients with colorectal cancer .
Output: Plasma prolactin
Plasma prolactin concentrations during incubation , 25 . 8 + / - 2 . 3 ng / ml , decreased to baseline levels , 10 . 8 + / - 1 . 9 ng / ml , within 24 hr after nestbox removal .
Output: Plasma prolactin
HBO had marked effects on these enzymes : lung SOD increased ( guinea pigs 47 % , rats 88 % ) and CAT and GSHPx activities decreased ( 33 % ) in brain and lung .
Output: lung SOD
Serum C3 was measured in 1 , 282 school children with abnormal urinary findings between 1980 and 1997 .
Output: Serum C3
Finally , the conservation of the two residues most sensitive to mutations ( Y949 and Y953 ) in TM11 , and in the homologous TM5 , of all mammalian P - gps and also in other ABC transporters , suggests that these residues and domains may play an important role in structural as well as mechanistic aspects common to this family of proteins .
Output: ABC transporters
This cell line expresses productively rearranged immunoglobulin genes as well as the Oct - 2 gene at high levels which are comparable to those observed in activated murine splenic B cells .
Output: immunoglobulin genes
The glucose effect on the pyruvate kinase gene is reversibly antagonized by agents increasing intracellular cAMP .
Output: pyruvate kinase gene
The cause for the increase in plasma ANP levels in the active phase remains to be determined .
Output: plasma ANP
Dominant - negative upf1 mutations were isolated following in vitro mutagenesis of a plasmid containing the UPF1 gene .
Output: UPF1 gene
The strongly DNA binding p50 subunit showed only very weak , if any , induction of gene expression .
Output: p50 subunit
The majority of putative TSC2 mutations were found in sporadic rather than TSC2 - linked families .
Output: TSC2 mutations
In contrast , a similar fusion protein ( hGH - LDLR - DAF17 , abbreviated HLD ) containing a fragment of the serine / threonine - rich domain of the LDL receptor ( LDLR ) in place of the DAF - derived serine / threonine - rich sequences , does not become GPI anchored .
Output: LDL receptor
The classic sterol regulatory cis element ( sre - 1 ) in the LDL receptor promoter mediates sterol regulatory element binding protein ( SREBP ) - binding and the effects of insulin and platelet derived growth factor ( PDGF ) .
Output: LDL receptor
Interleukin - 1 increased the noradrenaline release .
Output: Interleukin - 1
It was found that on the 14th and 21st day after cimetidine administration serum gastrin levels were significantly elevated .
Output: serum gastrin
Selective upper abdominal sympathectomy increased basal acid output in rats but was without effect on stimulated acid output , serum gastrin concentration , and gastric mucosal histidine decarboxylase activity .
Output: serum gastrin
Developmental regulation of expression of the malate synthase gene in transgenic plants .
Output: malate synthase gene
CAT assays demonstrated that overexpression of RXRalpha conferred the best RA response , consistent with our previous observation that 9 - cis - RA is more potent than all - trans - RA for inducing the expression of the AFP gene .
Output: AFP gene
The more severe the CRF , the more likely that total HDL and HDL2 cholesterol will be low .
Output: HDL2 cholesterol
Consistent with this , rh5 expression in R8 disappears when R7 cells are absent ( in sevenless mutant ) .
Output: sevenless mutant
Footprints on the HPV 18 enhancer show five protected regions with homologies to NF1 , AP1 and EFII transcription factor binding motifs .
Output: HPV 18 enhancer
These Tlr family members , unlike others reported to date , were identified within a genomic database .
Output: Tlr family members
Although polyubiquitin chains linked through Lys ( 29 ) of ubiquitin have been implicated in the targeting of certain substrates to proteasomes , the signaling properties of these chains are poorly understood .
Output: polyubiquitin chains
These results suggest that repetitive GAA sequences enhance splicing by binding a protein complex containing a sequence - specific RNA binding protein and a general splicing activator that , in turn , recruit additional SR proteins .
Output: SR proteins
To understand this dramatic effect , we examined the localization of SR proteins and found that SC35 was shifted to the cytoplasm in sky1Delta yeast , although this phenomenon was not obvious with ASF / SF2 , indicating that nuclear import of SR proteins may be differentially regulated by phosphorylation .
Output: SR proteins
Glutathione reductase activities in liver , kidney , lung , and brain were not affected by diet .
Output: Glutathione reductase
Glutathione reductase ( GR ) was purified from the cyanobacterium Anabaena PCC 7120 .
Output: Glutathione reductase
Avian EMILIN was extracted from 19 - day - old embryonic chick aortas and associated blood vessels and purified by ion - exchange chromatography and gel filtration .
Output: Avian EMILIN
Recently , we demonstrated that platelet - derived growth factor ( PDGF ) - beta receptor stimulation , but not various other growth factors , inhibits transcription of alpha1D - , but not alpha1A - or alpha1B - ARs , resulting in reduced norepinephrine - mediated SMC growth .
Output: alpha1B - ARs
Increased TRE DNA binding failed to lead to increased transactivation correlating with the inability of SB 203580 to increase phosphorylation of these AP - 1 proteins .
Output: AP - 1
Using a GTP - dependent , brefeldin A - sensitive in vitro AP - 1 binding assay , we have determined here the parameters of the AP - 1 binding reaction .
Output: AP - 1
Electromobility shift analysis using oligonucleotides encompassing the proximal , distal , and BED / AP - 1 - binding regions failed to demonstrate selective transactivation after CD2 signaling of LPMC .
Output: AP - 1
DESIGN : Activating protein - 1 ( AP - 1 ) and Tat - induced transcription were assessed using Jun and hybrid Tat / Jun - expression plasmids and reporter gene constructs which contained AP - 1 binding sites upstream of the rat prolactin TATAA element or an HIV - 1 LTR construct in which AP - 1 binding sites replaced the TAR element .
Output: AP - 1