niryuu/sni-each-converted huggingface dataset:
The RHO1 gene encodes a homolog of the mammalian RhoA small GTP - binding protein in the yeast Saccharomyces cerevisiae .
Output: RHO1 gene
This latter complex reacts with an antibody to serum response factor ( SRF ) and exhibits the same binding characteristics as purified SRF .
Output: serum response factor
Moreover , our recent findings on the Tc52 encoding gene underline the interest of genetic manipulation of T . cruzi , not only making it possible to use more closely an in vitro approach to find out how genes function , but also to obtain ' attenuated ' strains that could be used in the development of vaccinal strategies .
Output: Tc52 encoding gene
Differential transcription of plastome - encoded genes in the mesophyll and bundle - sheath chloroplasts of the monocotyledonous NADP - malic enzyme - type C4 plants maize and Sorghum .
Output: malic enzyme
The delta Mo + SV tumor DNAs from B - lineage tumors were typically rearranged at the immunoglobulin gene loci and contained germ line configurations of the T - cell receptor beta gene .
Output: immunoglobulin gene loci
But if one equalizes the upper limits of normal for both markers to a common 95 % specificity , the tumour - indicating sensitivity of CA 19 - 9 clearly surpasses that of CA 50 .
Output: CA 50
Decreased plasma level of antithrombin III was assumed to be one of the major factors underlying hypercoagulable state in nephrotic syndrome .
Output: antithrombin III
It is pathogenetically proved to use antithrombin III concentrate preparations with anti - and dysaggregatory properties , fibronectin preparations , trasylol or its analogs during complex preoperative preparation of patients .
Output: antithrombin III
The immunoreactive antithrombin III increased significantly while the increase in antithrombin III activity was insignificant in 20 male Caucasian Danes upon 3 weeks supplementation of the diet with 10 ml daily of a cod liver oil concentrate .
Output: antithrombin III
Fulminant hepatic failure in these cases could be characterized by : ( 1 ) rapid decrease in serum alanine transaminase ( ALT ) level after discontinuation of ecarazine , ( 2 ) prolonged jaundice despite discontinuation of ecarazine , ( 3 ) high incidence of anti - nuclear antibody ( ANA ) ( 57 % ) , and ( 4 ) histological findings of extensive hepatocellular necrosis ranging from bridging necrosis to massive necrosis .
Output: serum alanine transaminase
The detergent - solubilized complex oxidizes caldariella quinol at high rates and is completely inhibited by cyanide and by quinolone analogs , potent inhibitors of quinol oxidases .
Output: quinol oxidases
Serum creatinine kinase activity was 7 , 800 - 17 , 500 U / l , making acute muscle damage likely , probably rhabdomyolysis .
Output: creatinine kinase
Radioimmunoassay of plasma gonadotropins ; problems of specificity
Output: plasma gonadotropins
This difference between SP - 1 and SP - 2 peptides was not due to their differential uptake by cell , since approximately 100 times more SP - 2 peptide could be found in cytoplasmic extracts than SP - 1 peptide in experiments using radiolabeled peptides .
Output: SP - 1
The immediate 5 ' - flanking region was GC - rich with 3 SP - 1 - like and 2 AP - 2 sites identified in close proximity to the transcription start site .
Output: SP - 1
The shift from complex I to complex II seen only in SCM - 1 - producer T cell lines upon activation was completely suppressed by cyclosporin A .
Output: SCM - 1
In contrast , postmitotic neurons possessed extremely low levels of E2F1 protein as assessed by the electrophoretic mobility shift assay and Western blotting .
Output: E2F1 protein
In contrast to the p16 - mediated G1 block , G1 arrest mediated by the cdk inhibitors p21Cip1 or p27Kip1 cannot be bypassed either by inactivation of pRb or overexpression of E2F family members .
Output: E2F family members
Site - directed mutagenesis shows that sites throughout the IRE alter negative control and IRE - BP binding reflecting the fact that the footprint of the IRE - BP is over the entire IRE .
Output: IRE - BP
Our results showed that the 5 - hydroxytryptamine ( 5 - HT ) content in platelets was : ( 1 ) increased in the subgroup of anti - social alcoholics ; ( 2 ) transiently and differently altered in alcoholics compared to opiate addicts ; and ( 3 ) lowered in drinking alcoholics and normal in alcoholics who were drinking as well as smoking ( that may occur via MAO - B inhibition by smoke ) .
Output: MAO - B
The sequence of LZ321 matched that of RREB1 , a transcription factor that bound to a Ras responsive element ( RRE ) very different from the sequence with which we isolated LZ321 .
Output: Ras responsive element
Protease digestion and alkaline extraction of microsomes containing labeled mutant proteins further showed that segment 3 was sufficient for stable membrane anchoring of the glycoproteins , indicating that this segment may specify the transmembrane domain of the gB glycoprotein .
Output: gB glycoprotein
The WEB2 gene encodes a 1 , 522 - amino - acid protein with homology to nucleic acid - dependent ATPases .
Output: WEB2 gene
Genomic Southern blot analysis suggests that the human haploid genome contains a single Gs alpha gene .
Output: Gs alpha gene
The encoded 260 - amino - acid ( aa ) ( H1 - I ) and 240 - aa ( H1 - II ) polypeptides possess the typical tripartite organization of animal H1 histones , with variable N - and C - terminal domains flanking a conserved ' globular ' DNA - binding domain .
Output: animal H1 histones
Immunoprecipitation of cell lysates with anti - phosphotyrosine and immunoblotting showed phosphorylated forms of the mitogenic pathway proteins Shc and MAPK in addition to p185 ( neu ) , suggesting that the Ras to MAPK mitogenic pathway is activated .
Output: anti - phosphotyrosine
The Polycomb group proteins are involved in maintenance of the silenced state of several developmentally regulated genes .
Output: Polycomb group proteins
These results suggest that both the distinct DNA binding properties of PMLRAR homodimers and the sequestration of RXR by PMLRARs may contribute to the molecular mechanisms which underlie the pathogenesis of APL .
Output: PMLRAR homodimers
Serum amyloid A ( SAA ) is a plasma protein which has been associated with several diseases , including amyloidosis , arthritis , and atherosclerosis , and its abnormal expression , particularly in nonhepatic cells , is implicated in the pathogenesis of these diseases .
Output: Serum amyloid A
Together , these results implicate Fal1p in the 18S rRNA maturation pathway rather than in translation initiation .
Output: 18S rRNA
Thus , in addition to the previously characterized FAS - binding factor 1 interacting with the inositol / choline - responsive - element motif , a second motif common to the promoter regions of both FAS genes could be identified .
Output: FAS genes
Administration of SnCl2 and ZnSO4 ( group V ) resulted in a decrease of ALA - D activity and in an increase in CP - U .
Output: ALA - D
Purified phosphorylated PhoP ( PhoPP ) had a half - life of approximately 2 . 5 h , which was reduced to about 15 min by addition of the same molar amount of * PhoR ( the cytoplasmic region of PhoR ) .
Output: * PhoR
In the case of the pT181 plasmid , inactivation of the initiator RepC protein occurs by the attachment of an oligonucleotide to its active tyrosine residue .
Output: initiator RepC protein
These results suggest that camptothecin resistance in CEM / DOX cells is due to different mechanism ( s ) than topoisomerase - or P - glycoprotein - associated multidrug resistance .
Output: P - glycoprotein
In this report , we describe a patient with renal cell carcinoma who experienced ventricular tachycardia while undergoing treatment with high - dose bolus IL - 2 .
Output: IL - 2
One cytoplasmic target which reflects the functional state of the plastids is protein kinase C .
Output: protein kinase C
The protein kinase C inhibitor GF - 109203X abolished the activation by phorbol ester and inhibited the effect of CCK by 78 % but had no effect on EGF - activated MAPK activity .
Output: protein kinase C
In addition , both inhibitors blocked phosphatidylcholine hydrolysis and protein kinase C translocation .
Output: protein kinase C
The liver - specific sequence contains unique consensus sites for phosphorylation by cyclic AMP - dependent protein kinase and by protein kinase C .
Output: protein kinase C
In contrast to the restricted tissue expression of gonadotropin and TSH receptors in gonads and thyroid , respectively , LGR4 is expressed in diverse tissues including ovary , testis , adrenal , placenta , thymus , spinal cord , and thyroid , whereas LGR5 is found in muscle , placenta , spinal cord , and brain .
Output: TSH receptors
Extracellular regulated kinases ( ERK ) 1 and ERK2 are authentic substrates for the dual - specificity protein - tyrosine phosphatase VHR .
Output: tyrosine phosphatase
Blood pressure was controlled long term ( with / without diuretics / beta - adrenoreceptor blocking drugs ) in sixteen out of nineteen patients with mild - moderate hypertension .
Output: beta - adrenoreceptor
Mutations of the human PTEN gene .
Output: human PTEN gene
This enhancer element functioned in a position - and orientation - independent manner both on the Pax - QNR P0 promoter and the heterologous thymidine kinase promoter .
Output: thymidine kinase
In further support of the biological significance of these DNA - protein interactions , the IL2RA oligonucleotide from - 291 to - 245 proved to be sufficient in either orientation to confer PMA inducibility to the mitogen - insensitive thymidine kinase gene promoter in Jurkat cells .
Output: thymidine kinase
When linked to the herpes simplex thymidine kinase minimal promoter , this fragment acts as an enhancing element in Rcho but not GC cells .
Output: thymidine kinase
We have determined the molecular organization and transcription start points ( tsp ) for the murine gene ( TK ) encoding thymidine kinase .
Output: thymidine kinase
The mouse alpha A - CRYBP1 gene specifies a 2 , 688 - amino acid protein with 72 % amino acid identity to its human homologue , PRDII - BF1 .
Output: PRDII - BF1
Paradoxically there is a decrease in the level of soluble A beta secreted from the cells .
Output: soluble A beta
The fz - 1 and fz - 2 genes are widely expressed in rat tissues with the highest steady - state levels of mRNA in kidney , liver , heart , uterus , and ovary .
Output: fz - 1
fz - 1 and - 2 mRNA levels were greater in neonatal than in corresponding adult tissues .
Output: fz - 1
The contribution of residues outside the Ras binding domain of Raf ( RafRBD ) to Ras - Raf interaction and Ras - dependent Raf activation has remained unresolved .
Output: Ras binding domain
The D . discoideum proteins were entirely conserved over the four regions known to be important for GTP binding and all contained the C - terminal CAAX aa motifs shared by other Rho proteins .
Output: Rho proteins
Thus , transgene expression directed by both the human and mouse Rb promoters is restricted to a subset of tissues in which Rb is normally expressed during embryogenesis .
Output: mouse Rb promoters
We propose that optimal transcription of the gD gene depends on the interaction of ICP4 with multiple binding sites across the gene and cellular factors that recognize specific sequence elements in the promoter .
Output: gD gene
The occurrence of the different types of TCRB rearrangement patterns has implications for PCR - based clonality assessment and for PCR - based detection of minimal residual disease via TCRB gene analysis .
Output: TCRB gene
However the precise function of DNA - PK in DNA double - strand break repair is not known .
Output: DNA - PK
Coimmunoprecipitation analysis suggests that these residues likely contribute to the multimerization function required for homomeric complex formation or heteromeric complex formation with p50 in that no association of p65 delta with itself or with p50 was evident .
Output: p65 delta
The activated glucocorticoid receptor forms a complex with Stat5 and enhances Stat5 - mediated transcriptional induction .
Output: glucocorticoid receptor
Twelve patients of leprosy with arthritis and 161 patients without arthritis were studied for immunological parameters like immunoglobulins ( IgG , IgM , IgA ) , C - reactive proteins and rheumatoid factor .
Output: rheumatoid factor
A new simple identification of rheumatoid factor on nitrocellulose was developed that allows quantitative detection .
Output: rheumatoid factor
Antinuclear antibodies , rheumatoid factor and C - reactive protein in serum of normal women using oral contraceptives .
Output: rheumatoid factor
The effective use of superoxide dismutase from human erythrocytes in the late stages of experimental influenza infection
Output: superoxide dismutase
A surge in the rate of ascorbyl radical production , directly correlated with oxyradical stress and an abrupt fall in superoxide dismutase activity in the granuloma , indicates ' switching on ' of a free radical - dependent machinery for the formation of granuloma after vasectomy .
Output: superoxide dismutase
We report five of 13 evaluable patients undergoing allogeneic sibling BM or PBSC transplantation for MM between 1990 and 1997 who met the criteria for adjuvant alpha - IFN therapy .
Output: alpha - IFN
The use of specific somatic cell hybrids have allowed us to locate the YAC contig telomeric to the D3F15S2 locus , in a region which is frequently deleted in lung carcinomas .
Output: D3F15S2 locus
Elevated systolic blood pressure and high postglucose serum insulin levels showed an independent , significant association with left ventricular mass in female diabetic subjects .
Output: serum insulin
The p58 ( PITSLRE beta 1 ) protein kinase ( PK ) is a member of a large supergene family related to the master mitotic protein kinase , p34cdc2 .
Output: PITSLRE beta 1
Similarly , beta adrenoceptors blockade has been shown to be of value in achieving continence in a small group of patients .
Output: beta adrenoceptors
Prolongation of prothrombin time was not due to depletion of vitamin K - dependent coagulation factors or manifest fibrinolysis , but due to the presence of circulating fibrinogen fibrinmonomer - FDP complexes .
Output: fibrinogen fibrinmonomer
Whereas mutant 6C4 specified a wild - type - size Pol protein , we detected no full - length Pol protein in 7E4 - infected cell extracts .
Output: mutant 6C4
7 - Nitroindazole ( 7NI ) , a relatively selective neuronal nitric oxide synthase ( nNOS ) inhibitor . was intraperitoneally administered to the guinea pigs 30 minutes before the onset of local anoxia .
Output: nitric oxide synthase
These include not only antioxidants , N - methyl - D - aspartate receptor antagonists , inhibitors of glutamate release , calcium channel blockers , polyamine antagonists , and nitric oxide synthase inhibitors , but cannabinoids , aspirin , melatonin , and vitamin B - 12 .
Output: nitric oxide synthase
Human plastin genes .
Output: Human plastin genes
These data suggest that the secreted , truncated receptor encoded by the 2 . 6 - kb c - erbB transcript can bind to TGF alpha and may play an important growth - regulatory function in vitro .
Output: TGF alpha
Human parvovirus B19 gene expression from the viral p6 promoter ( B19p6 ) is restricted to primary human hematopoietic cells undergoing erythroid differentiation .
Output: viral p6 promoter
The consensus mammalian ER stress response element ( ERSE ) conserved among grp promoters consists of a tripartite structure CCAAT ( N9 ) CCACG , with N being a strikingly GC - rich region of 9 bp .
Output: grp promoters
( We observed a ferritin value as high as 47 mg / L in one patient . ) Results with all these kits did not inter - compare well for ferritin concentrations greater than 300 micrograms / L , a finding that casts further doubt on the controversial use of serum ferritin measurement in cases of iron overload .
Output: serum ferritin
Relation between circulating immune complexes and serum ferritin in hemosiderosis of different etiologies
Output: serum ferritin
A higher percentage of children with cerebral malaria ( 40 per cent ) than with non - cerebral malaria ( 29 per cent ) or controls ( 20 per cent ) also had either serum ferritin < 100 micrograms / l and inflammation or sTfR > 7 . 3 mg / l or both .
Output: serum ferritin
Pancreatic expression of the glucagon gene depends on multiple transcription factors interacting with at least three DNA control elements : G1 , the upstream promoter element , and G2 and G3 , two enhancer - like sequences .
Output: glucagon gene
Nucleotide sequence of the gag gene and gag - pol junction of feline leukemia virus .
Output: gag gene
The yjr041c delta haploid cells gave rise to microcolonies comprising about 20 to 50 cells .
Output: yjr041c delta
Consistent with this observation , introduction of Smad sites into a TGFbeta - insensitive LEF1 / TCF target gene confers cooperative TGFbeta and Wnt responsiveness to the promoter .
Output: Smad sites
An additional stimulation of D1 receptors by giving SKF38393 30 min later produced an almost continuous pattern of jaw openings but less closure movements from the rest position , and the openings were accompanied by frequent tongue protrusions .
Output: D1 receptors
Effects of long - term use of raloxifene , a selective estrogen receptor modulator , on thyroid function test profiles .
Output: estrogen receptor
The nucleotide sequence of the estrogen receptor gene of Oreochromis aureus ( OaER ) indicates that the hormone - binding E domain is composed of 4 exons interspersed by short introns of only 0 . 18 - 1 . 3 kb each .
Output: estrogen receptor
A selective transcriptional induction system for mammalian cells based on Gal4 - estrogen receptor fusion proteins .
Output: estrogen receptor
Heterodimerization mutant RXR failed to alter GFP - VDR and nlsGFP - VDR distribution or activity .
Output: Heterodimerization mutant RXR
The pattern of expression of this and other Chi26 / Chi33 chimeric promoters suggest that the E - region contains cis - acting sequences which activate transcription in aleurone and silence transcription in leaves .
Output: E - region
Characterization and targeted disruption of murine Nup50 , a p27 ( Kip1 ) - interacting component of the nuclear pore complex .
Output: murine Nup50
The effects of dopamine and of dopamine D2 receptor blocker haloperidol on the activity of carotid chemoreceptors were studied in 24 anesthetized , paralyzed and artificially ventilated newborn kittens aged 0 - 17 days .
Output: dopamine D2 receptor
Protein sequencing , electrophoretic mobility shift assay , and immunoblot analyses identify p54 and p47 / 48 as members of the hepatocyte nuclear factor 3 ( HNF3 [ forkhead ] ) family of transcription factors .
Output: p47 / 48
Reversal of ' refractory septic shock ' by infusion of amrinone and angiotensin II in an anthracycline - treated patient .
Output: angiotensin II
Titres of antistreptolysin O in mothers of children affected with Down ' s syndrome .
Output: antistreptolysin O
Blood coagulation Factor X and its activated form Factor Xa play an essential role in the midphase of the clotting cascade .
Output: Factor Xa
In four calves given Haemonchus contortus larvae , the serum pepsinogen concentration rose quickly to reach a mean of 3 . 5 iu tyrosine on day 14 after infection .
Output: serum pepsinogen
As to the side effects of SISO , cylindruria with aggravation of microscopic hematuria and elevations of GOT , GPT and A1 - P were observed each one of them , respectively .
Output: A1 - P
No transactivation of the ovalbumin promoter ( pLovTATA ) template control was observed .
Output: ovalbumin promoter
The three OAS genes are flanked by markers WI - 10614 ( cen ) and D12S2293 ( tel ) and are contained within three sets of overlapping cosmid clones .
Output: OAS genes
Amniotic fluid ionic concentration in response to chronic fetal vasopressin infusion .
Output: fetal vasopressin
Acanthamoeba myosin I heavy chain kinase ( MIHCK ) phosphorylates the heavy chains of amoeba myosins I , increasing their actin - activated ATPase activities .
Output: amoeba myosins I
Parathyroid hormone responses of cyclic AMP - , serum - and phorbol ester - responsive reporter genes in osteoblast - like UMR - 106 cells .
Output: Parathyroid hormone
LCCH1 was identical to the Rdl gene , a known GABA receptor subunit gene from D . melanogaster , whereas LCCH2 and LCCH3 were novel D . melanogaster sequences that exhibited structural similarity to other members of the ligand - gated chloride channel gene family .
Output: Rdl gene
Consistent with this hypothesis , stimulation of A ( 2A ) - R or PKA enhanced nuclear aPKC activity .
Output: nuclear aPKC
To gain additional insights into this novel apoptotic checkpoint , we have now characterized the mouse survivin locus .
Output: mouse survivin locus
It was previously shown that Vi antigen expression was regulated by a system similar to the rcs regulatory system involved in colanic acid synthesis in Escherichia coli .
Output: Vi antigen
Various stimuli inactivate IkappaB alpha by triggering phosphorylation of the N - terminal residues Ser32 and Ser36 .
Output: IkappaB alpha
The highly conserved region of U6 snRNA has a structural similarity with the catalytic domain of the negative strand of the satellite RNA of tobacco ring spot virus [ ( - ) sTRSV ] , suggesting that the highly conserved region of U6 snRNA forms the catalytic center .
Output: U6 snRNA
The prgX gene is adjacent to prgQ which provides the promoter for prgB expression .
Output: prgX gene
We have previously shown that UNC - 4 expression in the VA motor neurons specifies the wild - type pattern of presynaptic input .
Output: UNC - 4
psrA mutants of both Pseudomonas species lost the ability to induce rpoS expression at stationary phase , but they retained the ability to produce quorum - sensing autoinducer molecules .
Output: psrA mutants
Unconventional mRNA processing in the expression of two calcineurin B isoforms in Dictyostelium .
Output: calcineurin B isoforms
Searching the human DNA data base of expressed sequence tags ( EST ) revealed novel partial sequences similar to , but distinct from , the sequences of the previously known PEF proteins .
Output: PEF proteins
Using beta 1 - AR - luciferase reporter recombinants , we have previously determined that important beta 1 - AR genetic elements controlling expression within the C6 glioma cell line are contained within the region - 396 to - 299 , relative to the translational start site .
Output: luciferase reporter
Concerning the practical approach in a clinical setting , it has to be pointed out that with these diseases a hepatitis C infection has to be considered and testing for hepatitis C antibodies and , if positive , hepatitis C - RNA is indicated .
Output: hepatitis C antibodies
Promoter elements and transcriptional control of the mouse acetylcholinesterase gene .
Output: mouse acetylcholinesterase gene
Activation of the cytotactin promoter by the homeobox - containing gene Evx - 1 .
Output: cytotactin promoter
Interestingly , basal MAPK , but not Raf - 1 , activity was constitutively enhanced in Jak1 - deficient HeLa cells .
Output: Raf - 1
An in vitro Raf - 1 kinase assay , however , failed to detect LPS - induced Raf - 1 kinase activity in RAW 264 . 7 cells , suggesting that in RAW 264 . 7 cells , Raf - 1 kinase is not an activating component of the LPS signaling pathway regulating MAPK activity or sIL - 1Ra promoter activity .
Output: Raf - 1
Inhibition of Raf - 1 signaling by a monoclonal antibody , which interferes with Raf - 1 activation and with Mek substrate binding .
Output: Raf - 1
Co - operativity of functional domains in the muscle - specific transcription factor Myf - 5 .
Output: Myf - 5
We identified two potential phosphorylation sites at serine49 and serine133 , both of which seem to be necessary for Myf - 5 activity .
Output: Myf - 5
To elucidate the molecular mechanism by which the Ras signaling pathway activates a cell - type - specific gene , we have used the pituitary - specific rat prolactin ( rPRL ) promoter as a target of oncogenic Ras and Raf in GH4 rat pituitary cells .
Output: oncogenic Ras
Furthermore , we have identified the major open reading frame ( RF4 ; 2 . 3 kb ) as being essential for activation , and we have shown that the NF kappa B , SP1 , and TATA box motifs in the human immunodeficiency virus LTR are all required for full induction of the promoter by the HHV - 6 - encoded transactivator .
Output: NF kappa B
We therefore investigated whether the promoter activity of the mouse TRH gene is directly regulated by RA using a transient transfection assay into CV - 1 cells .
Output: mouse TRH gene
Taken together , these results indicate that ICSBP and PU . 1 are critical elements for IL - 18 gene expression .
Output: PU . 1
The findings in the human multiple myeloma cell lines represent the first examples of B cells with downregulated PU . 1 expression and apparently contradict observations in the murine system in which PU . 1 is expressed and active in plasmacytoma cell lines .
Output: PU . 1
YDL003w ( also termed MCD1 ) is a homologue of Schizosaccharomyces pombe rad21 , an essential gene implicated in DNA double - strand break repair and nuclear organization in fission yeast .
Output: Schizosaccharomyces pombe rad21
Using gel retardation analysis , four binding sites for Rap1p have been identified within the promoter of the RAP1 gene .
Output: RAP1 gene
The present report shows that a cis - acting element between - 189 and - 175 bp , which binds thyroid transcription factor - 1 ( TTF - 1 ) , is involved in both activities .
Output: TTF - 1
Negative modulation of alpha1 ( I ) procollagen gene expression in human skin fibroblasts : transcriptional inhibition by interferon - gamma .
Output: interferon - gamma
Soggy , a spermatocyte - specific gene , lies 3 . 8 kb upstream of and antipodal to TEAD - 2 , a transcription factor expressed at the beginning of mouse development .
Output: TEAD - 2
Spen is the only known homeotic protein with RNP binding motifs , which indicates that splicing , transport , or other RNA regulatory steps are involved in the diversification of segmental morphology .
Output: homeotic protein
These results suggest that the Reg1 - Glc7 phosphatase is a cytoplasmic component of the machinery responsible for returning Snf1 kinase activity to its basal level and reestablishing glucose repression .
Output: Snf1 kinase
Recently we have performed a detailed analysis of specific neuronal populations affected by the mutation which shed new light on the role of Krox - 20 in the segmentation and on the physiological consequences of its inactivation .
Output: Krox - 20
We have thus cloned two yeast homologs of mammalian p220 .
Output: mammalian p220
From a chromosomal cosmid library of Streptomyces argillaceus , a Mtm producer , a clone ( cosAR7 ) was isolated by homology to the actI / III region of S . coelicolor and the strDEM genes of S . griseus .
Output: strDEM genes
Fluorescence in situ hybridization was used to investigate the physical distribution and revealed that both retrotransposon families are present on all sugar beet chromosomes and largely excluded from chromosomal regions harbouring the 18S - 5 . 8S - 25S rRNA genes .
Output: 5 . 8S
Amplification of 4q21 - q22 and the MXR gene in independently derived mitoxantrone - resistant cell lines .
Output: MXR gene
Interestingly , VDR was able to bind to VDREs with high affinity and to fully activate transcription in intact yeast cells in the presence of the retinoid X receptor ( RXR ) .
Output: retinoid X receptor
Transient transfection assays indicated that the ( - 4551 ) UCP1 - CAT construct , containing the 5 ' - regulatory region of the rat ucp - 1 gene , was activated by PPARalpha co - transfection in a dose - dependent manner and this activation was potentiated by Wy 14 , 643 and retinoid X receptor alpha .
Output: retinoid X receptor
Sequence and deletion analysis of the recombination enhancement gene ( ref ) of bacteriophage P1 : evidence for promoter - operator and attenuator - antiterminator control .
Output: recombination enhancement gene
The hematopoietic form of PTPN6 transcript is initiated at a downstream promoter separated by 7 kb from the first .
Output: PTPN6 transcript
To examine the effect of the cAMP signal transduction pathway on transcription of the gene encoding the catalytic subunit of glucose - 6 - phosphatase ( G6Pase ) , G6Pase - chloramphenicol acetyltransferase ( CAT ) fusion genes were transiently transfected into either the liver - derived HepG2 or kidney - derived LLC - PK cell line .
Output: chloramphenicol acetyltransferase
This transformation suppression by Rb was further shown to be due to transcriptional repression of neu using Rb expressing effector plasmid and neu promoter - chloramphenicol acetyltransferase reporter gene .
Output: chloramphenicol acetyltransferase
We have used the chloramphenicol acetyltransferase ( CAT ) reporter gene system to study the effect of T on the long terminal repeats ( LTRs ) of a large family of human endogenous retrovirus - like sequences , RTVL - H .
Output: chloramphenicol acetyltransferase
Two plasmids , one containing the amino terminus of P fused to the DNA - binding domain of the yeast transactivator , GAL4 , and the other containing the amino terminus of NP fused to the herpesvirus transactivator , VP16 , were transfected in COS - 1 cells along with a chloramphenicol acetyltransferase ( CAT ) reporter plasmid containing GAL4 DNA - binding sites .
Output: chloramphenicol acetyltransferase
This novel TRPC7 gene could be a candidate gene for genetic disorders such as bipolar affective disorder , nonsyndromic hereditary deafness , Knobloch syndrome , and holoprosencephaly , which were mapped to this region .
Output: TRPC7 gene
The major promoter element of the Xenopus laevis 5S RNA gene is located within the transcribed region of the gene and forms the binding site for the transcription initiation factor TFIIIA .
Output: 5S RNA
Antibodies against REAP - 1 inhibit in vitro RNA editing reactions confirming its role in RNA editing .
Output: REAP - 1
Plasma beta - thromboglobulin ( beta - TG ) , a platelet - specific protein , is a marker of intravascular platelet degranulation .
Output: beta - TG
The Sty1 kinase is stimulated by a variety of different stress conditions including osmotic and oxidative stress and heat shock .
Output: Sty1 kinase
Analysis of WT1 target gene expression in stably transfected cell lines .
Output: WT1 target gene
The exon sequences determined from genomic DNA sequencing showed some differences when compared to the published rat GLUT2 cDNA .
Output: rat GLUT2 cDNA
Two adjacent , highly homologous endoglucanase genes , celD and celE from Fibrobacter succinogenes S85 , which were separated by an AT - rich 223 - nucleotide intergenic region were characterized .
Output: homologous endoglucanase genes
This raises the possibility that recombination occurred between corresponding LTR and vif loci of the quasi - species present in the isolates described here .
Output: vif loci
Several 35 - mm slides of dystrophin - , laminin - , and concanavalin A ( ConA ) - stained muscle sections were used to calculate myofiber cross - sectional areas and to compare different techniques and settings of an image capture system .
Output: concanavalin A
Defective concanavalin A - induced suppression in bancroftian filariasis .
Output: concanavalin A
The expected products of the cloned bph genes , except bphA3 , were observed in E . coli in an in vitro transcription - translation system .
Output: bph genes
Base substitutions within this NFIL - 6 site resulted in virtual elimination of LPS - induced IL - 1 beta gene transcription .
Output: IL - 1
These findings suggest that IL - 1 - stimulated , Rho - dependent cytoskeletal reorganization may cluster signaling molecules in specific architectures that are necessary for persistent cell activation in chronic inflammatory disease .
Output: IL - 1
Both genes ARO3 and ARO4 are strongly regulated under the general control regulatory system .
Output: genes ARO3
VAV and SOCS1 form a protein complex through interactions between the VAV NH ( 2 ) - terminal regulatory region and the SH2 domain of SOCS1 in a phosphotyrosine - independent manner .
Output: SH2 domain
We were able to demonstrate a significant statistical interaction effect between gender and globulin protein concentration on Vu ( p = 0 . 022 ) .
Output: globulin protein
Adhesion was inhibited by mAbs against the COOH - terminus and central cell binding domains of fibronectin , as well as by the corresponding CS1 and RGD peptides .
Output: RGD peptides
Some CHOP - C / EBP heterodimers apparently bind to alternative DNA sequence and thereby regulate the transcription of other genes .
Output: C / EBP
However , C / EBP beta stimulated transcription primarily through the cAMP - responsive element ( CRE ) , which maps between positions - 77 to - 94 , but not at the more 5 ' - binding sites .
Output: C / EBP
Western blot analyses reveals that cJun and the C / EBP family member C / EBP - beta are physiologically relevant transcription factors whose expression corresponds with mda - 7 mRNA expression .
Output: C / EBP
Coexpression of C / EBP alpha altered the cell specificity .
Output: C / EBP
Cross - talk between transcription factors NF - kappa B and C / EBP in the transcriptional regulation of genes .
Output: C / EBP
Previous data suggested a subtle increase in serum P at the time of hCG injection without LH surge reduces the PR of women having oocyte retrievals for IVF ; this study compared PRs of recipients in a shared oocyte program according to the donors ' pre - hCG P level .
Output: pre - hCG
Finally , we tested whether activation by either of these factors is dependent on components of the SAGA complex .
Output: SAGA complex
The other end of this cluster contained the human type I cytokeratin K20 and K12 gene loci .
Output: K12 gene
When cotransfected with the HIV LTR CAT into CV - 1 cells , both the pCD41 and pGD41 clones trans - activated the HIV LTR .
Output: HIV LTR
In the HIV LTR , this region has been demonstrated to have both positive and negative regulatory effects on HIV gene expression .
Output: HIV LTR
The predicted amino acid sequence of the FlaB2 polypeptide was 92 % identical to that of T . pallidum FlaB2 , with a 76 % identity at the nucleotide level .
Output: FlaB2 polypeptide
Secretogranin II ( SgII ) is a secretory polypeptide stored in large dense core vesicles of neuroendocrine and neuronal cells .
Output: Secretogranin II
Further , cells cotransfected with a UF promoter - luciferase ( - 1935UF - Luc ) reporter gene and the BTEB expression vector had 2 - fold higher Luc activity than those cotransfected with reporter gene and pcDNA - 3 .
Output: UF promoter
There is little similarity among the sequences upstream from the CAP site of the Spec2 genes except the TATA consensus sequence and a repeating trinucleotide , AAC .
Output: Spec2 genes
An 81 - nt tandem duplication of the C - terminal coding region is located adjacent to the termination codon of the CyIIIb gene , a potential relic of a slipped mispairing and replication event .
Output: CyIIIb gene
By sequencing of exonuclease III deletion clones an open reading frame of 405 nucleotides was found coding for a protein of 135 amino acids with a molecular mass of 15 kDa .
Output: exonuclease III
Human CR2 has a restricted cellular distribution , being expressed on B lymphocytes , dendritic cells of the spleen , pharyngeal epithelial cells , and at low levels on some T lymphocytes .
Output: Human CR2
In vitro , c - Src phosphorylated FAK Tyr - 925 in a glutathione S - transferase - FAK C - terminal domain fusion protein , whereas FAK did not .
Output: c - Src
In cotransfection assays , a small fragment of Sin retaining the Src - SH3 - binding site and one tyrosine - containing motif induced c - Src activation as measured by a transcriptional reporter .
Output: c - Src
Transforming growth factor - beta ( TGF - beta ) inhibits cell cycle progression , in part through up - regulation of gene expression of the p21 ( WAF1 / Cip1 ) ( p21 ) cell cycle inhibitor .
Output: TGF - beta
Electrophoretic mobility - shift assays with nuclear extracts from COS - 1 cells transfected with expression vectors for SMADs 1 - 5 indicate that SMAD3 forms a complex with a migration similar to that of the endogenous TGF - beta - specific complex observed in fibroblast extracts .
Output: TGF - beta
Furthermore , CBP / p300 stimulated both TGF - beta - and Smad - induced transcription in a Smad4 / DPC4 - dependent fashion .
Output: TGF - beta
In addition to TAK1 , TGF - beta also stimulated JNK activity .
Output: TGF - beta
Similarly , antisense TGF - beta type II receptor oligodeoxynucleotides ( 40 microM ) resulted in a 58 % increase in branching , compared to scrambled and mismatched sequence controls , while TGF - beta , type II receptor mRNA and its protein expression levels were suppressed by 95 and 84 % , respectively .
Output: TGF - beta
Transforming growth factor beta activates the promoter of cyclin - dependent kinase inhibitor p15INK4B through an Sp1 consensus site .
Output: Sp1 consensus site
These mutant rps7 leaders were ligated into an aadA expression cassette and transformed into the chloroplast of C . reinhardtii and into E . coli .
Output: mutant rps7
YACs selected from our contig will be the starting point for the cloning of the LGMD2B gene and thereby establish the biological basis for this form of muscular dystrophy and its relationship with the other limb - girdle muscular dystrophies .
Output: LGMD2B gene
p107 induction of E2F DNA binding was observed primarily in S phase cells coincident with the increase in p107 protein levels .
Output: p107 protein
Although indomethacin is useful for examining the role of cyclooxygenase products in asthmatic responses , it should not be considered in the treatment of asthma .
Output: cyclooxygenase products
We have isolated and determined the complete nucleotide sequence of another operon , cpeCD , that encodes the linker proteins associated with phycoerythrin hexamers in the phycobilisome .
Output: phycoerythrin hexamers
Oral basal body temperature ( BBT ) recordings of 46 women that conceived by donor insemination and who had midcycle monitoring of luteinising hormone ( LH ) were analysed to establish features associated with an optimal cycle .
Output: luteinising hormone
Unlike these contaminant - responsive T cells , those that are truly specific for natural AChR epitopes appear less heterogeneous and therefore more suitable targets for selective immunotherapy .
Output: AChR epitopes
Optical rotation of the second harmonic radiation from retinal in bacteriorhodopsin monomers in Langmuir - Blodgett film : evidence for nonplanar retinal structure .
Output: bacteriorhodopsin monomers
Mutations abolishing MalK function not only result in inability to transport maltose but also cause constitutive expression of the maltose regulon .
Output: maltose regulon
Serum levels of angiotensin converting enzyme were well maintained .
Output: angiotensin converting enzyme
To assess the safety of the angiotensin converting enzyme inhibitor enalapril a multicenter , open , randomized , prazosin - controlled trial was designed comparing the incidence and severity of symptomatic hypotension on the first day of treatment .
Output: angiotensin converting enzyme
The N - terminal sequence of the extracellular MEP20 , TKVAS , was found at aa 194 - 198 within the ORF .
Output: extracellular MEP20
RESULTS : BMK - 1 and other 19 contents significantly decreased depolarization parameters Vmax , APA , OS , and MDP , which was similar to that of TTX and different from that of Nim and BaCl2 .
Output: BMK - 1
By computed homology search , we noticed significant similarities between US3 PK and p21 - activated kinase ( PAK ) , which is activated by the Cdc42 or Rac .
Output: US3 PK
Cell rounding was abrogated by expression of either kinase - dead forms of US3 PK or a mutant protein lacking the acidic cluster in the kinase regulatory domain .
Output: US3 PK
Sequences within and flanking hypersensitive sites 3 and 2 of the beta - globin locus control region required for synergistic versus additive interaction with the epsilon - globin gene promoter .
Output: beta - globin
Activation of the mitogen - activated protein kinase ( MAPK ) pathway enhances long - range transactivation by the beta - globin locus control region ( LCR ) ( W .
Output: beta - globin
These results point towards post - translational steric and / or allosteric control of EKLF function that may be important not just for its DNA binding ability , but also for its potential to interact with other proteins that fully establish the correct stereospecific array leading to efficient switching of beta - globin transcription during development .
Output: beta - globin
Herein , we report that CRE - decoy oligonucleotide treatment results in an increase in the p53 protein level in MCF - 7 human breast cancer cells that express wild - type p53 .
Output: p53 protein
Reduction of p53 protein was detected after 1 day of OM treatment and reached maximal suppression of 10 - 20 % of control after 3 days in H3922 and 40 % of control after 4 days in MCF - 7 cells .
Output: p53 protein
However , only plasma fibrinogen concentrations showed statistically significant positive associations with IMT in both groups .
Output: plasma fibrinogen
ET - 1 limited the electrocardiographic evidence of subendocardial ischemia and attenuated contractile dysfunction compared with mechanical stenosis at the same coronary flows , even though lactate flux was similar .
Output: ET - 1
Uptake of colicins required different domains in TonB , for colicin B and M around residue 160 and for colicin Ia , a domain closer to the C - terminal end .
Output: colicin Ia
To study the mechanisms and the signaling pathways involved , IGF - 1R promoter reporter constructs were transiently transfected in CHO - AT1 cells that overexpress angiotensin AT1 receptors .
Output: angiotensin AT1 receptors
These clones contain most of the exon of cyclin D2 except exon 5 .
Output: cyclin D2
Although no detectable phenotypes are associated with a disruption allele of ABP1 , mutations that create a requirement for this protein have now been isolated in the previously identified gene SAC6 and in two new genes , SLA1 and SLA2 .
Output: gene SAC6
Second , CGGA - containing sequences placed at - 88 in the delta MTV - CAT reporter plasmid conferred insulin responsiveness to the mammary tumor virus promoter .
Output: CAT reporter
This ligand - independent pathway can function through another androgen - regulated promoter as shown by the use of the mouse mammary tumor virus MMTV - CAT reporter .
Output: CAT reporter
In addition , because SB203580 had no effect of TNF - or ceramide - induced apoptosis , our results strongly argue against a role for p38 MAPK in the induction of TNF - - or ceramide - induced apoptosis .
Output: p38 MAPK
Although paclitaxel alone failed to induce p38 MAPK activation , subsequent ( but not prior ) exposure to PD98059 induced a dramatic increase in p38 MAPK phosphorylation .
Output: p38 MAPK
These data indicate that SB 203580 sensitive p38 MAP kinases are not involved in okadaic acid mediated increases in TRE DNA binding and transactivation .
Output: p38 MAP kinases
It competes with the calcium ion which brings about inhibition of myosine kinase , and therefore a drop in phosphorylated myosine .
Output: myosine kinase
Previously identified mutations in this variant have been within or close to the coding region of exon 1 of the HMBS gene , the only exon that is expressed solely in the ubiquitous isoform .
Output: HMBS gene
In contrast , corticotropin releasing factor ( CRF ) , injected centrally produces a suppression of punished and non - punished responding in the conflict test consistent with its hypothesized role in mediating behavioral responses to stress .
Output: corticotropin releasing factor
Sequence analysis revealed 100 % homology of all RA - derived PTEN fragments to those from normal SF as well as to the published GenBank sequence ( accession number U93051 ) .
Output: PTEN fragments
Previous work has shown that glucose repression of PRB1 transcription is not mediated by HXK2 or by the SNF1 , SNF4 , and SNF6 genes ( C .
Output: SNF6 genes
Like troponin Cs and calmodulins , PfCPK also contains four EF hand calcium - binding motifs .
Output: troponin Cs
Hpr1 forms , together with Tho2 , Mft1 , and Thp2 , the THO complex , which controls transcription elongation and genome stability in Saccharomyces cerevisiae .
Output: THO complex
Like other IAPs , ch - IAP1 contains the N - terminal baculovirus IAP repeats and C - terminal RING finger motifs .
Output: ch - IAP1
Visualization of the cells by phase contrast microscopy indicated that murine PKCepsilon expression in the presence of glycerol resulted in a significant increase in the number of yeast cells exhibiting very small buds .
Output: murine PKCepsilon
Comparison of the predicted amino acid sequence with that of the Drosophila virilis gene shows that several blocks of amino acid sequence have been very highly conserved .
Output: Drosophila virilis gene
A 5789 - nucleotide - long EcoRI fragment from the genome of Thermotoga maritima , identified by cross - hybridization to L11 , L1 , L10 , and L12 ribosomal protein gene sequences from Escherichia coli , was cloned and sequenced .
Output: EcoRI fragment
The 7 . 2 kb EcoRI fragment of AfMNPV was cloned and the nucleotide sequences of the polyhedrin coding region and its flanking regions were determined .
Output: EcoRI fragment
Restriction mapping showed that the two recombinant plasmids shared an EcoRI fragment of 8 . 9 kb .
Output: EcoRI fragment
Such tetranucleotides are common in promoter regions , particularly in activating transcription factor / cyclic AMP response element - binding protein ( ATF / CREB ) and E - box motifs , suggesting that PIF may modulate the transcription of many genes .
Output: activating transcription factor
Characterization of human activating transcription factor 4 , a transcriptional activator that interacts with multiple domains of cAMP - responsive element - binding protein ( CREB ) - binding protein .
Output: activating transcription factor
Regulation of activating transcription factor - 1 and the cAMP response element - binding protein by Ca2 + / calmodulin - dependent protein kinases type I , II , and IV .
Output: activating transcription factor
Increased gliadin antibody levels were found more frequently in patients with subtotal villous atrophy ( 9 out of 17 patients , or 53 % ; p less than 0 . 05 ) than in patients with partial villous atrophy ( 2 out of 13 patients , or 15 % ) or normal villous appearance ( 2 out of 10 patients , or 20 % ) .
Output: gliadin antibody
Action against Vibrio cholerae O1 Tox + of chemical products used in the lemon production .
Output: Tox +
Our results are consistent with TCOF1 mutations leading to the Treacher Collins syndrome phenotype .
Output: TCOF1 mutations
Although mutant pex5delta cells expressing a yeast / tobacco Pex5p chimaera failed to import a GFP - Eci1p reporter protein , they were able to grow on oleic acid .
Output: mutant pex5delta
Two genes ( ptsI and ptsA ) that encode homologues of the energy coupling Enzyme I of the phosphoenolpyruvate - dependent sugar - transporting phosphotransferase system ( PTS ) have previously been identified on the Escherichia coli chromosome .
Output: Enzyme I
It contains 31 exons and a high proportion of class 0 introns , alternative splicing of which results in significant levels of variant transcripts that maintain the original open reading frame of MRP mRNA .
Output: MRP mRNA
Hepatocyte growth factor ( HGF ) is an inducible cytokine that is essential for the normal growth and development of various tissues , such as the liver .
Output: Hepatocyte growth factor
The protein encoded by the NF2 gene has a similarity to ezrin , radixin and moesin ( ERM ) proteins that link membrane proteins to the cytoskeleton .
Output: NF2 gene
The dopamine D1 dopamine receptor antagonist SCH 23390 dose dependently ( 7 . 5 - 30 micrograms / kg s . c . ) antagonized the stimulant locomotor effect on both drugs but did not prevent their antiimmobility effect on the behavioral despair test .
Output: dopamine receptor