Query: magnetic field  catalase

title: EFFECT OF WEAK MAGNETIC FIELD AND LOW LEVEL LASER RADIATION ON ENDOCELULLAR CATALASE AND PEROXIDASE ACTIVITY OF FUNGI ACTIVE DESTRUCTORS OF POLYMERIC MATERIALS

title: Modification of catalase and MAPK in Vicia faba cultivated in soil with high natural radioactivity and treated with a static magnetic field.
abstract: The effects of a static magnetic field (SMF) and high natural radioactivity (HR) on catalase and MAPK genes in Vicia faba were investigated. Soil samples with high natural radioactivity were collected from Ramsar in north Iran where the annual radiation absorbed dose from background radiation is higher than 20mSv/year. The specific activity of the radionuclides of (232)Th, (236)Ra, and (40)K was measured using gamma spectrometry. The seeds were planted either in the soil with high natural radioactivity or in the control soils and were then exposed to a SMF of 30mT for 8 days; 8h/day. Levels of expression of catalase and MAPK genes, catalase activity and H2O2 content were evaluated. The results demonstrated significant differences in the expression of catalase and MAPK genes in SMF and HR treated plants compared to the controls. An increase in catalase activity was accompanied by increased expression of its gene and accumulation of H2O2. Relative expression of the MAPK gene in treated plants, however, was lower than those of the controls. The results suggest that the response of V. faba plants to SMF and HR may be mediated by modification of catalase and MAPK.

title: Effect of Combined Pulsed Magnetic Field and Cold Water Shock Treatment on the Preservation of Cucumbers During Postharvest Storage
abstract: The aim of this study was to evaluate the combined effect of pulsed magnetic field (PMF) and cold water shock pretreatment on the quality of postharvest cucumbers. Cucumbers were immersed into cold water at 0.5 degC with PMF for 40 min and then stored at 6 degC for 13 days. Different levels of the PMF (0.0, 2.0, 4.0, and 6.0 mT) were applied with a frequency of 25 Hz. The results showed that combined pretreatments of PMF and cold water possessed better preservation quality and prolonged their shelf life. When the magnetic flux density of PMF was 4.0 mT, the color change and decay rate of samples were the lowest and decreased by 52.8% and 73% at 13 days. The preservation mechanism of combined PMF and cold water may be ascribed to triggering higher catalase activity, which induced higher membrane integrity that displayed lower malondialdehyde accumulation.

title: Alternation of Growth, Phenolic Content, Antioxidant Enzymes and Capacity by Magnetic Field in Hyssopus officinalis under Water Deficit
abstract: In the present study, the effect of seed priming with magnetic field (MF; 45, 90, 200 and 250mT for 5, 10, 20 and 30 min) was evaluated in 60 day old Hyssopus officinalis plants grownunder 8 days irrigation intervals. The assessments were consisted of biomass, membranestability, photosynthetic pigments concentrations, polyphenols content, antioxidant enzymesactivities and antioxidant capacity. In comparison with the exclusively water stressed plants,MF priming significantly altered these parameters, particularly at 200 mT/5 min. At thisintensity, the level of biomass, total chlorophyll and polyphenols content increased by 2.2, 2.5and 7.7 folds, respectively. Furthermore, electrolyte leakage and MDA content decreased by35 and 33% Reducing power, DPPH and superoxide anion scavenging activities highlyaugmented by MF. MF priming at 200 mT increased catalase +92% and ascorbateperoxidase +2.3 folds) activities. But, the highest activity of guaiacol peroxidase wasrecorded for MF primed H. officinalis at 90 mT. In conclusion, seed priming with MFincreases drought tolerance in H. officinalis through protection of cellular membrane integrity,maintenance of photosynthetic pigments content and also alternation of antioxidant enzymeactivities. It also improves medicinal properties of the shoots via increasing polyphenolsconcentration and antioxidant capacity.

title: Establishment and assessment of cell suspension cultures of Matricaria chamomilla as a possible source of apigenin under static magnetic field
abstract: This study represents an optimized protocol for callus establishment and cell suspension culture of Matricaria chamomilla, and the impact of the static magnetic field (SMF) on flavonoid metabolism and antioxidant activity were examined for the first time. The effect of growth regulators was investigated to enhance biomass growth and apigenin production. Murashige and Skoog medium supplemented with 2,4 D (1.5 mg l 1) and Kinetin (0.5 mg l 1) showed the highest callus induction rate (100% fresh weight, apigenin (0.82% and apigenin 7 glucoside (1.57% contents. Cell suspension culture was established, and the optimum subculture time was found to 13 15 days. SMF induced cell leaching and oxidative stress in all treated cells by an increase in H2O2 content and more stimulation of superoxide dismutase (SOD) catalase (CAT) and peroxidase (POX) enzymes activities. Total phenolic, flavonoid and DPPH activity increased in cells treated to SMF, and the maximum content of apigenin (1.3% and apigenin 7 glucoside (2.1% were identified in cell treated to 4 mT. These results provided an effective method for the regulation of flavonoid biosynthesis in M. chamomilla cell suspension culture, and the use of SMF as a tool for the induction of apigenin production. Cell suspension cultures of Matricaria chamomilla contain valuable medicinal flavonoids. Static magnetic field promoted apigenin production and antioxidative enzyme activities in M. chamomilla cell suspension.

title: The investigation of the effect of NAD, H2O2 and weak magnetic field on the antibacterial mechanism of isoniazid (INH) that first line antibiotic against M. tuberculosis agent
abstract: Tuberculosis is an infectious disease, which is caused by the Mycobacterium tuberculosis complex. This disease leads to up to 1.3 million deaths out of more than eight million cases every year. A prodrug called isoniazid has been proven to be effective and widely used in the treatment of infections caused by tuberculosis. Despite its use for more than six decades clinically, the action mechanism of this prodrug is yet to be elucidated. INH action agains mycobacteria requires catalase peroxidase (KatG) function, and IN NAD adduct formation is catalyzed in vitro by M. tuberculosis KatG under a variety of conditions. Low intensity EMF (Electromagnetic Field) has been used in the2rapeutic practices in addition to its use in telecommunication systems and food protection. EMF is used in medicine and food industries especially for its bactericidal effects. In this study, we aimed to investigate the effects of weak magnetic field application and the addition of NAD and H2O2 on the action mechanism of isoniazid. We added H2O2 and NAD individually and together, to the different groups at varying concentrations. Also, one experimental group was exposed to a 5mT, 50Hz magnetic field for 4 to 5 hours per day (total of 45 hours in 10 days) The agar proportion method was used to evaluate the results. It was determined that the addition of 100 mM NAD and H2O2 together increased the effectiveness of isoniazid to some extent. However, the application of a weak magnetic field did not change the effectiveness of the drug.

title: Biochemical and biomolecular effects induced by a static magnetic field in Saccharomyces cerevisiae: Evidence for oxidative stress
abstract: Exposure to static magnetic fields (SMF) can cause changes in microorganism metabolism altering key subcellular functions. The purpose of this study was to investigate whether an applied SMF could induce biological effects on growth of Saccharomyces cerevisiae, and then to probe biochemical and bio molecular responses. We found a decrease in growth and viability under SMF (250mT) after 6h with a significant decrease in colony forming units followed by an increase between 6 h and 9 h. Moreover, measurements of antioxidant enzyme activities (catalase, superoxide dismutase, glutathione peroxidase) demonstrated a particular profile suggesting oxidative stress. For instance, SOD and catalase activities increased in magnetized cultures after 9 h compared with unexposed samples. However, SMF exposure caused a decrease in glutathione peroxidase activity. Finally, SMF caused an increase in MDA levels as well as the content of protein carbonyl groups after 6 and 9 h of exposure.

title: Cold Shock Resistance of Activated Sludge Microorganisms Strengthened by a Static Magnetic Field
abstract: We studied the anti cold shock performance of activated sludge microbes (ASM) with magnetic field (MF) strengthening under slow and rapid cooling and rewarming. From stage S3 to S4, the TTC DHA (triphenyltetrazolium chloride dehydrogenase) of reactor A2 and B2 were 98.5% and 72.5% higher than that of reactor A1 and B1, which showed that MF had a better strengthening effect on TTC DHA under slow temperature variation than that under rapid temperature variation. MF had a definite strengthening effect on relief and inhibition of cell membrane lipid peroxidation and low temperature injury, which was indicated by SOD (superoxide dismutase) CAT (catalase) and MDA (malonaldehyde) while the MF strengthening effect on SOD and CAT activity was not stable under rapid cooling and rewarming. The MF had little effect on phospholipid fatty acid (PLFA) diversity of ASM under slow cooling, but had higher PLFA diversity on that under rapid cooling. Optimal MF strengthening application under different temperature variation mode is supposed to be a potential pathway to strengthen the activity and cold resistance of ASM and improve the efficiency of wastewater treatment in low temperatures.

title: Minimize Magnetic Field disorders by using Antioxidants
abstract: The study aimed to investigate the treatment effect of marjoram leaves on brain tissue and some biochemical properties that exposed to magnetic field (MF) Magnetic fields may cause heating of the skin and the tissue, which could lead to several disorders as increase the production of reactive oxygen species. Animals have the effective enzymatic antioxidant defense system including catalase (CAT) superoxide dismutase (SOD) and Glutathione (GSH) and Glutathione reductase (GSH Px) enzymes. Lipid peroxidation in blood (erythrocytes) was measured as the amount of malon dialdehyde (MDA) This system allow for scavenging of reactive oxygen species ROS and leading to protection of cells from oxidative damage. Fruits, plants and vegetables contain high amounts of antioxidants, like polyphenols, vitamin C, vitamin E, b carotene, and lycopene. Plants as Marjoram contains several acids as urosolic acid, carnostic acid, and carnosol which used as free radical scavengers. So it protect organs as liver brain and kidney and enhance the antioxidant activities

title: Responses of alfalfa influenced by magnetic field and rhizobial inoculant
abstract: Plants are generally subjected to a combination of different conditions such as magnetic field and soil bacteria in their life. The present investigation tried to compare the effects and interactions of magnetic field and rhizobial inoculant in alfalfa. A pot experiment was performed under a natural condition by a factorial design to investigate the influences of magnetic field with 0.75 and 1.5 mT intensities on treated and untreated alfalfa seed with Sinorhizobium meliloti. Results showed that 1.5 mT magnetic intensity reduces growth parameters, protein content, catalase, ascorbate peroxidase, and peroxidase activity as a stress factor while superoxide dismutase activity, malondialdehyde, and reducing sugars content increased. Interaction of rhizobial inoculant and 1.5 mT intensity can reduce the damage caused by magnetic field generated in the plant. Application of 0.75 mT intensity and rhizobial inoculant (individual and together) led to a significant increase in growth parameters, protein content, catalase, ascorbate peroxidase, peroxidase, and superoxide dismutase enzyme activity while malondialdehyde and reducing sugars content reduced. Therefore, as an eco friendly technique in agriculture, the application of 0.75 mT and rhizobial inoculant might improve the plant quality. This method could be used as a biofertilizer for vegetable production which reduces the environmental pollution caused by the application of biochemical fertilizers.