Query: Codtus igneus

title: Primary Screening of Phytochemicals Present in Crude Extracts of Leaf, Stem and Rhizome of Costus igneus (Insulin Plant)
abstract: Phytochemicals are secondary metabolites produced by all plants, some of which are of great medicinal value. Primary screening of phytochemicals is a crucial step in the detection of the bioactive compounds present in medicinal plants, and consequently may lead to drug discovery and development. Qualitative analysis was done to investigate the active phytoconstituents present in Costus igneus. In the present study, leaf, stem and rhizome extracts were prepared with different solvents, viz. ethanol, methanol, acetone, ethyl acetate and chloroform. A total number of 19 phytochemicals, viz. alkaloids, cardiac glycosides, flavonoids, saponins, tannins, terpenoids, anthraquinones, reducing sugars, glycosides, phlobatannins, organic acids, proteins, amino acids, steroids, carbohydrates, oils resins, phenols, carboxylic acid and quinones were tested for their presence or absence in the different parts of the plant. Ethanol and methanol proved to be better solvents than others, as the crude extracts showed the presence of more number of phytochemicals like, flavonoids, alkaloids, anthraquinones, taninns, phenolic compounds, terpenoids, saponins, carbohydrates and proteins etc. Other solvents showed the presence of fewer phytochemicals. Leaves and rhizome were found to contain more phytochemicals when compared to stem. These phytochemicals will be of great value in the herbal drug industry.

title: Effect of Costus igneus Leaf Juice on Antidiabetic Activity of Glimepiride in Rats A Suggestive Study
abstract: March 2019  Due to therapeutic efficacy, Costus igneus Nak. (CI) (popularly known as insulin plant) is one medicinal plant in the limelight of having cure for diabetes. An oral diabetic agent Glimepiride was selected to evaluate the activity in per se and in combination with Costus igneus. The study drugs (glimepiride or Costus igneus leaf juice) were administered orally and blood glucose levels were determined using Glucometer at stipulated time intervals. It perhaps works in pathophysiologic/metabolic deranged conditions as observed by its per se effect in streptozotocin induced diabetic rats' contrast to its effect in normal rats. The results of our study confirm that, the combined action of glimepiride and Costus igneus leaf juice has greater antidiabetic effects than their individual effects (per se)

title: In Vitro Cytotoxicity Activity of Ethanolic Leaf Extract of Costus Igneus Against Hepatocellular Carcinoma (HepG2) Cells
abstract: Costus igneus popularly known as 'Insulin plant' belongs to Costaceae family. The anti diabetic potential of the plant has been evaluated and widely used as Ayurvedic medicinal herb to treat diabetes mellitus and associated metabolic syndrome. Recently Non alcoholic fatty liver disease (NAFLD) associated metabolic syndrome has been identified as an important risk factor for hepatocellular carcinoma (HCC) So agents with insulin sensitizing action could play dual role in the control of metabolic syndrome and cancer. This study is designed to evaluate the in vitro anticancer activity of the ethanolic extract of Costus igneus leaves against hepatocellular carcinoma( HepG2) cells. The viable cells were assessed by cytotoxicity activity (MTT assay) with the eight different concentration (1000 to7.8mg/ml) of the extracts. The percentage of viability was calculated. From the graph the concentration required for a 50% of viability (IC50) was calculated as 62.5mg/ml. However 74.57% and 22.65% of cell viability were produced by the concentration of 7.8mg/ml and 1000mg/ml respectively. The results showed the cytotoxic activity of ethanolic extract of Costus igneus leaves and proved the anticancer activity against liver cancer cells.

title: Antioxidant and cytotoxic potential of leaf extracts of Costus igneus
abstract: Costus igneus leaves are traditionally used to treat diabetes and this plant is commonly known as insulin plant.  The purpose of this study was to evaluate the antioxidant and cytotoxic potential of different leaf extracts of C. igneus.  The antioxidant potential of various leaf extracts of C. igneus was established by ferric reducing antioxidant power (FRAP) assay, 2,2' azino bis (3 ethylbenzothiazoline 6 sulfonic acid) (ABTS) assay, metal chelating activity, phosphomolybdenum assay, superoxide radical scavenging activity, 2,2 diphenylpicrylhydrazyl (DPPH) assay, reducing power assay, and cytotoxic activity by 3 (4,5 dimethylthiazol 2 yl) 2,5 diphenyltetrazolium bromide (MTT) assay on breast cancer cell line Michigan Cancer Foundation (MCF 7)  In FRAP assay, acetone extract showed higher activity of 276.31 mmol Fe (II) E/mg extract. In ABTS assay, hexane extract showed the highest scavenging activity of 12878.893 mM Trolox equivalent antioxidant capacity (TEAC)/g extract. Hot water extract registered higher metal ion chelating ability with 2.94 mg ethylenediaminetetraacetic acid equivalent/g extract. Hexane extract has recorded the highest total antioxidant capacity of 65.00 mg AAE/mg in phosphomolybdenum assay. Superoxide radical scavenging activity was found maximum in acetone extract with 41.11% In DPPH assay, acetone extract recorded total free radical scavenging capacity with IC50 value of 40.70 mg/ml. Hexane extract has recorded significant highest reducing power at concentration 100 mg/ml with maximum optical density of 0.177. Acetone extract of C. igneus leaf extract at concentration of 150 mg/ml showed the highest cytotoxic activity on cancer cell line with cell viability of 65.51%  The present study explored that C. igneus shows efficient antioxidant activity as well as cytotoxic activity and could act as safe and cost effective with potential biological applications.

title: ANTIDIABETIC POTENTIAL OF Costus igneus LEAF IN STREPTOZOTOCIN INDUCED DIABETIC WISTAR ALBINO RATS

title: POTENTIAL THERAPEUTIC EFFICACY OF COSTUS IGNEUS FOR DIABETES MELLITUS OTHER DISEASES WITH FRAMEWORK FOR ITS EVALUATION BASED ON AYURVEDA
abstract: 1. Asst. Professor, Dept. of Kriya Sharir, IPGT RA, Jamnagar. 2. Professor, Dept. of Kriya Sharir, Y.A.C. Kodoli, Maharashtra. Manuscript Info Abstract Manuscript History Received: 10 October 2019  December 2019

title: Phytochemical Analysis and Antibacterial Determination of Costus Igneus Leaves

title: In Silico Analysis of Micro Propagated Endangered Species Costus Igneus

title: on Medicinal Exploration of Costus igneus The Insulin plant C Review
abstract: Unauthorised republication, reproduction, distribution, dissemination and copying of this document in whole or in part is strictly prohibited. Available online at www.globalresearchonline.net 51 Flowerlet Mathew, Bimi Varghese 1. Assistant Professor, Department of Pharmaceutics, Nirmala College of Pharmacy, Kerala, India. 2. Department of Pharmaceutics, Nirmala College of Pharmacy, Muvattupuzha P.O, Kerala, India. Corresponding author's E mail: flowerletmathew@gmail.com

title: Influence of solvents on the changes in structure, purity, and in vitro characteristics of green synthesized ZnO nanoparticles from Costus igneus
abstract: The present study is intended to produce high purity zinc oxide nanoparticles from the leaves of Costus igneus and zinc acetate precursor via sustainable methods by the tribulation with three different solvents (hot water, methanol, and acetone) for the extraction of plant compounds. While examining the physico chemical characteristics of ZnO nanoparticles incurred by the catalysis of plant bioactive compounds extracted from different solvents, the hot water extract based green synthesis process yields higher purity (99.89% and smaller particle size (94 nm) than other solvents. The optimization of the solvents used for the green synthesis of nanoparticles renders key identification in appropriate extraction of bioactive compounds suitable for the nucleation/production of nanoparticles in addition to annealing temperature. The impregnable usage of ZnO nanoparticles in clinical applications is further confirmed based on the treatment of particles (1 10 mg ml 1) against Gram positive (S. aureus and S. epidermis) and Gram negative bacteria (E. coli and K. pneumoniae) with respect to their growth inhibition. An in force growth inhibition against particular S. aureus and S. epidermis imparted by the low concentration of ZnO nanoparticles signifies the utilization and consumption of green synthesized high purity nanoparticles for therapeutic and cosmetic applications.