Query: Saccharomyces cerevisiae sheep

title: Saccharomyces cerevisiae (YT001) supplementation for the control of Haemonchus contortus and modulation of the immune response of sheep.
abstract: Studies aiming at the development and evaluation of alternative methods to minimise losses caused by the gastrointestinal nematode Haemonchus contortus are extremely important. Such research is essential, given the high morbidity rates among sheep and the significant mortality rates of lambs, allied to the low efficacy of commercial products for the control of this parasite. The purpose of this study was to evaluate the effect of the Saccharomyces cerevisiae (YT001 YEASTECH) on the control of H. contortus and its modulation of the immune response in experimentally infected sheep. Eighteen sheep were divided into two groups. Group 1, the control group, comprised animals infected with H. contortus and supplemented with distilled water, while Group 2, the treated group, consisted of animals infected and supplemented with S. cerevisiae (400 million cfu/day of suspension for 49 days) The following parasitological parameters were evaluated: number of eggs per gram of faeces, number of infective larvae (L3) recovered per faecal culture, and parasitic load of the abomasum. The following immunological parameters were quantified: immunoglobulin (Ig)A in the mucous secretions and serum IgG; cytokines interleukin (IL) 4, IL 5 and IL 10; number of eosinophils in the abomasal mucosa and groups of cells positive for the markers: MHCII, CD4+CD25+ CD5+CD8+ WC4, CD5+CD4+ CD8+CD11b+ and CD5+WC1 by whole blood flow cytometry. The results revealed a significant decrease (P<0.05) in the number of larvae and significantly higher serum IgG levels (P<0.05) in the group supplemented with S. cerevisiae. The supplemented animals showed significantly larger numbers of eosinophils (P<0.05) as well as more cells positive for MHCII, CD4+CD25+ CD5+CD8+ than the control animals. This study confirmed the beneficial action of S. cerevisiae on the host immune response to H. contortus, as evidenced mainly by the smaller number of L3 recovered from the faeces of sheep supplemented with S. cerevisiae.

title: Growth Performance and Nutrient Digestibility by Sheep Fed Diets Containing Yeast (Saccharomyces cerevisiae)
abstract: The aim of this study was to determine the effects of varying dietary levels of yeast (Saccharomyces cerevisiae) on growth performance and nutrient digestibility by West African dwarf (WAD) sheep. Forty eight lambs (24 males and 24 females) were used in a complete randomized 2x3 factorial design. The sheep were assigned to high roughage and high concentrate diets supplemented yeast (0, 0.75 and 1.5 g of Saccharomyces cerevisiae per kg of the basal diets) Sheep fed HC diets supplemented with Saccharomyces cerevisiae had significantly (p 0.05) higher average daily feed intake, average daily weight gain, and final body weight than those fed other treatment diets. Dietary inclusion of S. cerevisiae improved feed conversion ratio significantly (p 0.05) Supplementation of S. cerevisiae to HC diet increased (p 0.05) significantly the dry matter, crude protein, and crude fibre digestibility coefficients. It was concluded that S. cerevisiae can be added into HC diet at 0.75 g per kg of the diet for optimum performance of WAD sheep.

title: Evaluation of biochemical profile and rumen fluid parameters of sheep supplemented with Saccharomyces cerevisiae and subjected to an abrupt diet change
abstract: This study examines rumen fluid parameters and the biochemical profile of feedlot sheep supplemented with a commercial product composed of yeast culture and enzymatically hydrolyzed yeast during an abrupt change of diet. Eight sheep at 30 months of age, with an average weight of 40.2 3.4 kg, were housed in individual stalls where they received the same diet twice daily, which was formulated according to the National Research Council [NRC] (2007) The animals were divided into two groups, namely, supplemented with Saccharomyces cerevisiae (SG, n 4) and control (CG, n 4) The experiment consisted of three periods: the first (seven days) consisted of an acclimation to the roughage only diet; in the second period (five days) the animals of SG started to receive supplementation; lastly, in the third period (five days) all animals underwent a sudden change of diet, which was characterized by an alteration from 100% roughage to a 50:50 ratio (roughage:concentrate) without prior adaptation. Blood samples were collected to evaluate albumin, aspartate amino transferase, calcium, gamma glutamyl transferase, globulins, glucose, magnesium, potassium, sodium, total plasma proteins and urea. Rumen fluid was harvested to evaluate physical aspects. Supplementation increased the protozoa count; the rate of sedimentation and fluctuation remained within the physiological parameters; and total plasma proteins and globulin decreased. Therefore, the yeast appears to be a promising alternative for reducing the deleterious effects of an abrupt change of diet.

title: Effect of Levels of Feeding and Saccharomyces Cerevisiae on Some Haematological and Biochemical Indices in West African Dwarf Sheep

title: EFFECT OF LEVELS OF ANIMAL DIETARY PROTEIN AND PROBIOTIC ADDITIVES (SACCHAROMYCES CEREVISIAE AND BACILLUS SUBTILIS) ON INSULIN, TSH AND GROWTH HORMON OF AWASSI SHEEP
abstract: To maintain the body on its metabolic activities, including catabolic and anabolic processes, it needs tools. Insulin, thyroxine, growth hormone and other hormones are considered the tools that contribute to regulate metabolism in living organism. The studies that interest in that scope and applied on ruminants are too scarce, therefore the current research has studied the effect of levels of dietary protein and probiotic additives (Live Saccharomyces cerevisiae more than 3.0x1013 CFU and bacillus subtilis more than 4.0 x109CFU) on insulin, TSH and growth hormone of Awassi sheep. In this study was used sixteen sheep, animals have divided to four groups (four animals in each group) The first group fed with crude protein(cp) (12% with no probiotics, the second group fed with cp (12% plus 1 kg/ton probiotic, the third group fed with cp (14% with no probiotics, and finally the fourth one fed with cp (14% plus 1 kg/ton probiotic. The main results have shown that there is significant increase (P<0.01) in the insulin concentration between class cp (12% comparing with class cp (14% Regarding the interaction treatments, there is a significant increase (P<0.05) between cp (12% plus no probiotic comparing with cp (14% plus no probiotic and with cp (14% plus probiotic, and between cp (12% plus probiotic comparing with cp (14% plus probiotic. Concerning TSH, the results shows that there is a significant increase (P<0.01) between cp (12% plus no probiotic comparing with cp (12% plus probiotic and with cp (14% plus no probiotic, and between cp (14% plus probiotic comparing with cp (12% plus probiotic and with cp (14% plus no probiotic. Regarding growth hormone, results haven't shown any significant differences among any classes whether in main effects or in interaction treatments. We could conclude that, there are significant effects of dietary protein levels plus probiotic additives on insulin and TSH concentrations but not on growth hormone concentration.

title: Growth performance and nutrient digestibility of West African Dwarf Sheep fed high roughage diet containing Saccharomyces cerevisiae
abstract: A twelve week study was conducted to determine the effects of dietary inclusion of yeast Saccharomyces cerevisiae on growth performance and nutrient digestibility of West African dwarf (WAD) sheep. A total of 12 lambs (6 rams and 6 ewes) were randomly allotted to three treatment diets as follows: treatment I was a high roughage diet with no inclusion of S. cerevisiae treatment II was a high roughage diet with 0.75 g of S. cerevisiae per kg of diet; treatment III was a high roughage diet with 1.5 g of S. cerevisiae per kg of diet. The chemical composition of the high roughage diet is as follows: dry matter (89.20% organic matter (85.87% crude protein (13.21% crude fibre (16.40% ether extract (3.30% ash (4.33% nitrogen free extract (51.96% neutral detergent fibre (45.25% and acid detergent fibre (26.62% Each group was made up of four replicates with one sheep serving as a replicate. There were significant p 0.05) affected. There were significant p 0.05) differences among treatments in dry matter (DM) organic matter (OM) crude protein (CP) crude fibre (CF) neutral detergent fibre (NDF) and acid detergent fibre (ADF) digestibility coefficients values. Based on these results addition of 1.5 g of S. cerevisiae per kg of diet is recommended.  yeast, ruminant, protein, faeces, fibre

title: Saccharomyces cerevisiae mannan induces sheep beta defensin 1 expression via Dectin 2 Syk p38 pathways in ovine ruminal epithelial cells
abstract: The rumen epithelium of sheep serves as an immune interface with the environment and secretes antimicrobial peptides with bactericidal function against various pathogens. Sheep beta defensin 1 (SBD 1) an antimicrobial peptide, is secreted from ovine ruminal epithelial cells (OREC) in response to microbial infections. Mannan, the main component of the Saccharomyces cerevisiae cell wall can stimulate innate and regulatory immune responses that could improve the gastrointestinal environment. We aimed at investigating the effects of mannan on SBD 1 expression and the downstream signaling pathways stimulated in OREC. We cultured OREC; assessed the effects of mannan on SBD 1 expression by qPCR and ELISA; and then investigated the underlying signaling pathways using qPCR, ELISA, Western blotting, immunohistochemistry, and immunohistofluorescence. Interestingly, mannan markedly upregulated SBD 1 expression in a concentration and time dependent manner. Dectin 2 Mouse mAb, Syk specific inhibitor R406, and specific inhibitors of the p38, ERK1/2, JNK, and NF kB pathways attenuated mannan induced SBD 1 expression to varying degrees. These results demonstrate that SBD 1 is upregulated by mannan via the Dectin 2 Syk axis, and this is regulated to a large extent through the mitogen activated protein kinase (MAPK) p38 and less so through the ERK1/2 and JNK or the NF kB pathway. Our findings highlight the immunomodulatory effects of mannan on OREC in terms of mannan induced SBD 1 expression.

title: Growth, lipid profile and methane production by sheep fed diets containing yeast (Saccharomyces Cerevisiae)
abstract: A twelve week study was conducted to determine the effects of dietary inclusion of yeast (Saccharomyces cerevisiae) on growth performance, lipid profile and methane production by West African dwarf sheep. Twenty four (24) lambs(12 males and 12 females) were randomly allotted to six treatment diets in a 3x2 factorial arrangement involving grass (Panicum maximum) hay, grass/ legume mixture (50:50) hay and legume (Centrosema pubescens) hay either without or with S. cerevisiae supplementation (1.5g/ kg diet) Result showed that the average daily feed intake, average daily weight gain and final body weight were significantly higher for sheep fed legume diet with S. cerevisieae supplementation than those fed other diets. Sheep fed grass diet without supplementary yeast had significantly higher methane emission than those fed other diets. Based on the results, the addition of S. cerevisiae to legume diet was recommended to improve feed intake, daily weight gain, feed efficiency and methane emission.

title: Effect of Yeast (Saccharomyces cerevisiae) Supported by Selenium and Zinc on Lipid Profile of Local Sheep Males
abstract: This study was conducted to investigate the effect of (Saccharomyces cerevisiae) supported with zinc and selenium on the lipid profile. of Twenty one Iraqi males sheep were used aged (1 1.5) years and the weight mean approximately (38+ 4) kg. experiment animals used divided randomly into three treated groups each group have 7 males. All males were fed on the same ration. The first group was considered as a control group without treatment (T1) the second group (T2) were given yeast enriched with zinc (Saccharomyces cerevisiae zinc) with dosage (0.2 g/kg/bw) the third group (3) was given yeast enriched with selenium (Saccharomyces cerevisiae selenium) with dosage (0.03 g/kg/bw) The results showed significant (P<=0.05) differences control group compare with the other two treated groups (T3 and T2) in triglyceride, LDL VLDL and HDL The results indicate to significant differences (P<=0.05) of (T2 and T3) compare with the control group by HDL From the results concluded that there synergistic effect of Saccharomyces cerevisiae with zinc and selenium as organic form (Saccharomyces cerevisiae zinc/selenium) could be attributed to improved animal health.

title: Hematological and Biochemical Indices of West African Dwarf Sheep Fed Diets Containing Yeast (Saccharomyces cerevisiae) Grass, Grass/Legume (50:50) and Legume