Query: ESRE

title: The evolutionarily conserved ESRE stress response network is activated by ROS and mitochondrial damage
abstract: Mitochondrial dysfunction causes or contributes to a wide variety of pathologies, including neurodegenerative diseases, cancer, metabolic diseases, and aging. Cells actively surveil a number of mitochondrial readouts to ensure that cellular homeostasis is maintained.  In this article, we characterize the role of the ethanol and stress response element (ESRE) pathway in mitochondrial surveillance and show that it is robustly activated when the concentration of reactive oxygen species (ROS) in the cell increases. While experiments were mostly performed in Caenorhabditis elegans we observed similar gene activation profile in human cell lines. The linear relationship between ROS and ESRE activation differentiates ESRE from known mitochondrial surveillance pathways, such as the mitochondrial unfolded protein response (UPR mt which monitor mitochondrial protein import. The ability of the ESRE network to be activated by increased ROS allows the cell to respond to oxidative and reductive stresses. The ESRE network works in tandem with other mitochondrial surveillance mechanisms as well, in a fashion that suggests a partially redundant hierarchy. For example, mutation of the UPR mt pathway results in earlier and more robust activation of the ESRE pathway. Interestingly, full expression of ATFS 1, a key transcription factor for the UPR mt requires the presence of an ESRE motif in its promoter region.  The ESRE pathway responds to mitochondrial damage by monitoring ROS levels. This response is conserved in humans. The ESRE pathway is activated earlier when other mitochondrial surveillance pathways are unavailable during mitochondrial crises, potentially to mitigate stress and restore health. However, the exact mechanisms of pathway activation and crosstalk remain to be elucidated. Ultimately, a better understanding of this network, and its role in the constellation of mitochondrial and cellular stress networks, will improve healthspan.

title: ESRE Input to the European Commission for Horizon Europe " Space " Work Programme 2021 2022

title: ESRE reflections on space related R&TD and its governance in Horizon Europe

title: ESRE Network Activation and Regulation Provide Insight into Mitochondrial Surveillance

title: Differential quantitative proteomics reveals the functional difference of two yigP locus products, UbiJ and EsrE
abstract: The yigP (ubiJ) locus has been shown to be associated with many phenotypic changes in Escherichia coli, while the individual function of its two products, EsrE small RNA and UbiJ protein, is still elusive. In this study, we constructed two single element mutants, EsrE mutant strain Mut and UbiJ mutant strain Ter, on the basis of the base substitution programs. The variable antibiotics resistance and ubiquinone (UQ, coenzyme Q) yield and the similar cell growth between mutants revealed the division of labor and collaboration of EsrE and UbiJ in JM83. Furthermore, we detected the concentration of intracellular proteins of Mut and Ter by stable isotope labeled quantitative proteomics. The results demonstrate that both EsrE and UbiJ are involved in the aerobic growth of E. coli, while EsrE preferentially contributes to the amino acid related pathway, and UbiJ is an indispensable factor in the biosynthesis of UQ. Moreover, we uncovered a potential regulatory circuit of d cycloserine (DCS) that composed of EsrE, GcvA, and GcvB by proteomic analysis.

title: sRNA EsrE is transcriptionally regulated by the ferric uptake regulator Fur in Escherichia coli.
abstract: Small RNAs (sRNAs) are widespread and play major roles in regulation circuits in bacteria. Previously, we have demonstrated that transcription of esrE is under the control of its own promoter. However, the regulatory elements involved in EsrE sRNA expression are still unknown. In this study, we found that different cis regulatory elements exist in the promoter region of esrE. Then, seven potential corresponding trans regulatory elements are screened and analyzed by using pull down assays based on DNA affinity chromatography. Among these candidate regulators, we investigate the relationship between the ferric uptake regulator (Fur) and the EsrE sRNA. Electrophoresis mobility shift assays (EMSAs) and b galactosidase activity assays demonstrated that Fur can bind to the promoter region of esrE, and positively regulate EsrE sRNA expression in the presence of Fe2+

title: EsrE A yigP Locus Encoded Transcript Is a 3' UTR sRNA Involved in the Respiratory Chain of E. coli
abstract: The yigP locus is widely conserved among g proteobacteria. Mutation of the yigP locus impacts aerobic growth of Gram negative bacteria. However, the underlying mechanism of how the yigP locus influences aerobic growth remains largely unknown. Here, we demonstrated that the yigP locus in Escherichia coli encodes two transcripts; the mRNA of ubiquinone biosynthesis protein, UbiJ, and the 3' untranslated region small regulatory RNA (sRNA) EsrE. EsrE is an independent transcript that is transcribed using an internal promoter of the yigP locus. Surprisingly, we found that both the EsrE sRNA and UbiJ protein were required for Q8 biosynthesis, and were sufficient to rescue the growth defect ascribed to deletion of the yigP locus. Moreover, our data showed that EsrE targeted multiple mRNAs involved in several cellular processes including murein biosynthesis and the tricarboxylic acid cycle. Among these targets, sdhD mRNA that encodes one subunit of succinate dehydrogenase (SDH) was significantly activated. Our findings provided an insight into the important function of EsrE in bacterial adaptation to various environments, as well as coordinating different aspects of bacterial physiology.

title: Association of European Space Research Establishments ESRE Recommendations related to Framework Programme 9 I Budget Structure of FP 9 European Innovation Council
abstract: Also for FP9 there is a strong political willingness to prioritize the research via a "mission" oriented approach, where the "missions" will be mostly of a political societal or global nature (e.g. "decarbonization" "circular economy" "electrification of transport".in pillar III) but sometimes also of a scientific or technological nature (e.g. "understanding and enhancing the human brain until 2030" "first quantum computer in Europe" etc. in pillars I/II) The mission oriented approach would particularly foster multidisciplinary projects to be funded.

title: Esre: A novel essential non coding RNA in Escherichia coli
abstract: YigP gene (GeneID: 948915) locates between ubiquinone biosynthetic genes ubiE and ubiB in Escherichia coli. GeneBank annotates yigP as a putative protein coding gene. In this study, we found a new essential sRNA gene, esre, locates within the region of yigP. The E. coli strain with inactive esre must rely on a complementary plasmid to survive. Moreover, RACE experiments showed esre encodes an RNA molecule of 252 nt. Further experiments revealed esre gene is immune to frame shift mutations and the function of esre depends mostly on the RNA secondary structure, which are typical traits of sRNA. Since it is difficult to predict the target of an essential sRNA, more research is needed to reveal the function and mechanism of esre.

title: Coupling and Coordination Measurement and Interactive Analysis of ESRE System in CPER
abstract: Based on the coupling and coordinative characteristics of economic,society,resource and environment(ESRE)system in Central Plains Economic Region(CPER),taking economic,society,resource and environment as three composite systems respectively,through the quantitative integration measure,the paper aims at probing into the coupling interaction and coordinating relationship of ESRA system of Central China.The results show that the coupling degree of ESRE system is low,and the time sequence of coupling degree in CPER is at resisting stage from 1995 to 2012.The coupling coordination degree of ESRE system rose from 0.351 3 to 0.434 9in 1995 2012,and coordination intensity was in the middle level.The order degree of economic and social system had a more substantial increase with the improvement of new urbanization level,while the order degree of resource and environment system was in a fluctuated status.This shows that the sped up process of economic and social system in CPER in several years has brought more and more pressure to its environment system,so it is necessary to correctly handle and coordinate the relationship between the rapid developing urbanization and the increasingly severe environmental condition in CPER,and the measures should be taken as soon as possible to urge the development direction of the coupling ESRE system coordinately.From the spatial distribution status,the coupling coordination degree is similar to comprehensive coordination index,which presents Northwest Southeast distribution pattern.The spatial distribution pattern of coupling degree is different from coupling coordination degree and comprehensive coordination index,and some individual level was in inverted state.The main influencial factors of coupling coordination degree are still economic and social factors,although the resource and environment factors has some effect on coupling degree but little effect on the overall coupling coordination degree.