Towards Automated Cellular Image Segmentation for RNAi Genome-Wide Screening
The Rho family of small GTPases is essential for morphological changes during normal cell development and migration, as well as during disease states such as cancer. Our goal is to identify novel effectors of Rho proteins using a cell-based assay for Rho activity to perform genome-wide functional screens using double stranded RNA (dsRNAs) interference. We aim to discover genes could cause the cell phenotype changed dramatically. Biologists currently attempt to perform the genome-wide RNAi screening to identify various image phenotypes. RNAi genome-wide screening, however, could easily generate more than a million of images per study, manual analysis is thus prohibitive. Image analysis becomes a bottleneck in realizing high content imaging screens. We propose a two-step segmentation approach to solve this problem. First, we determine the center of a cell using the information in the DNA-channel by segmenting the DNA nuclei and the dissimilarity function is employed to attenuate the over-segmentation problem, then we estimate a rough boundary for each cell using a polygon. Second, we apply fuzzy c-means based multi-threshold segmentation and sharpening technology; for isolation of touching spots, marker-controlled watershed is employed to remove touching cells. Furthermore, Voronoi diagrams are employed to correct the segmentation errors caused by overlapping cells. Image features are extracted for each cell. K-nearest neighbor classifier (KNN) is employed to perform cell phenotype classification. Experimental results indicate that the proposed approach can be used to identify cell phenotypes of RNAi genome-wide screens.

title: Workflow-enabled distributed component-based information architecture for digital medical imaging enterprises
abstract: Few information systems today offer a flexible means to define and manage the automated part of radiology processes, which provide clinical imaging services for the entire healthcare organization. Even fewer of them provide a coherent architecture that can easily cope with heterogeneity and inevitable local adaptation of applications and can integrate clinical and administrative information to aid better clinical, operational, and business decisions. We describe an innovative enterprise architecture of image information management systems to fill the needs. Such a system is based on the interplay of production workflow management, distributed object computing, Java and Web techniques, and in-depth domain knowledge in radiology operations. Our design adapts the approach of "4+1" architectural view. In this new architecture, PACS and RIS become one while the user interaction can be automated by customized workflow process. Clinical service applications are implemented as active components. They can be reasonably substituted by applications of local adaptations and can be multiplied for fault tolerance and load balancing. Furthermore, the workflow-enabled digital radiology system would provide powerful query and statistical functions for managing resources and improving productivity. This paper will potentially lead to a new direction of image information management. We illustrate the innovative design with examples taken from an implemented system.

title: 76-Space analysis of grey matter diffusivity: Methods and applications
abstract: Diffusion Weighted Imaging (DWI) and Diffusion Tensor Imaging (DTI) are widely used in the study and diagnosis of neurological diseases involving the White Matter (WM). However, many neurological and neurodegenerative diseases (e.g., Alzheimer's disease and Creutzfeldt-Jakob disease) are generally considered to involve the Grey Matter (GM). Investigation of GM diffusivity of normal aging and pathological brains has both scientific significance and clinical applications. Most of previous research reports on quantification of GM diffusivity were based on the manually labeled Region of Interests (ROI) analysis of specific neuroanatomic regions. The well-known drawbacks of ROI analysis include inter-rater variations, irreproducible results, tediousness, and requirement of a priori definition of interested regions. In this paper, we present a new framework of automated 76-space analysis of GM diffusivity using DWI/DTI. The framework will be evaluated using clinical data, and applied for study of normal brain, Creutzfeldt-Jakob disease and Schizophrenia.

title: Stability Analysis of RED Gateway with Multiple TCP Reno Connections
abstract: It has been observed that a bottleneck random early detection (RED) gateway becomes oscillatory when regulating a flow in multiple TCP connections. The stability boundary of the TCP-RED system depends on various network parameters, making the adjustment of the RED gateway a difficult task. Based on a fluid-flow model, analytical conditions were formulated that describe the stable boundary of the RED gateway depending on the number of TCP Reno connections. The proposed model accurately generates a stability boundary surface in a four dimensional space, which facilitates the adjustment of parameters for stable operation of the RED gateway. The accuracy of the analytical results has been verified using the ns-2 network simulations.

title: Continuous Gesture Recognition using a Sparse Bayesian Classifier
abstract: An approach to recognise and segment 9 elementary gestures from a video input is proposed and it can be applied to continuous sign recognition. An isolated gesture is recognised by first converting a portion of video into a motion gradient orientation image and then classifying it into one of the 9 gestures by a sparse Bayesian classifier. The portion of video used is decided by using a sampling technique based on condensation framework. By doing so, gestures can be segmented from the video in a probabilistic manner. Experiments show that the proposed method can achieve accuracy around 90% in both isolated and continuous gesture recognition without using special equipment such as glove devices and the system can run in real-time

title: Optimum design of very low distortion class E power amplifiers
abstract: In this paper, the optimum design of a class E power amplifier with resonant tank being symmetrically driven by two class E circuits is studied. The optimally designed symmetrical class E circuit has extremely low harmonic distortion, and the load matching network (if required) is non-critical and can be designed with ease. Practical steady-state design equations are derived and graphically presented. Experimental circuits are constructed for distortion evaluation.

title: Spurious modulation on current-mode controlled DC/DC converters: an explanation for intermittent chaotic operation
abstract: We explain the mechanism that causes "intermittent" instability and chaos in a current-mode controlled switching converter. The circuit model used to study the phenomenon incorporates a coupling process through which a spurious signal is coupled to the current sensing and ramp compensation circuitry, resulting in a modulation of the compensation slope which causes the system to become unstable intermittently. We describe a way to find the parameter boundaries where intermittent chaotic operation emerges.

title: Design patterns for lazy evaluation
abstract: We propose an object-oriented (OO) formulation and implementation of lazy/delayed evaluation by reusing and extending an existing linear recursive structure (LRS) framework with the help of the strategy, decorator and factory design patters. The result is a robust, flexible framework that can handle both infinite and finite lists and to which existing algorithms for finite lists can be applied without modification. The OO techniques used to develop this model are effective tools for teaching abstraction and design of data structures.