Significance of EMSA Eritin Administration on Erythropoiesis and Complement Regulators in Irradiated Mice

OZ Amac: Bu calismada Balb/c farelerde 500 rad doz ile total vucut irradyasyonu (TVI) sonrasi poliherbal eritropoez modulator ve stimulator ajan (EMSA) Eritin uygulamasinin TER 119+VLA 4+ hucrelerine gore eritropoez aktivitesi ve TER 119+CD55+ ile TER 119+CD59+ hucrelerine gore kompleman regulatorleri modulasyonu uzerindeki etkisi incelendi. Gerec ve yontemler: Dusuk doz (1.04 mg/g BW) normal doz (3.125 mg/g BW) ve yuksek doz (9.375 mg/g BW) icerecek sekilde iki hafta boyunca gunde 500 rad dozunda TVI'ye maruz kalan farelere oral yoldan EMSA Eritin uygulandi. Fareler pozitif kontrol olarak haftada iki kere 0.21 mg/g BW dozunda HEMAPO Epoetin alfa ile tedavi edildi. On besinci gunde kemik iligi izole edildi ve akis sitometrisi kullanilarak TER 119+VLA 4+ TER 119+CD55+ ve TER 119+CD59+ hucrelerin sayisi analiz edildi. Inceleme, 500 rad dozunda TVI sonrasi farelerin kemik iligindeki TER 119+VLA 4+ hucrelerin sayisinda saglikli kontrollere gore anlamli azalma oldugunu gosterdi. Radyasyona maruz kalan farelerde EMSA Eritin ile TER 119+VLA 4+ hucrelerin sayisi TVI olan fareler ve Epoetin alfa ile tedavi edilen farelere gore dusuk dozdan yuksek doza anlamli sekilde artti.  En yuksek dozda EMSA Eritin eritropoezi desteklemede HEMAPO Epoetin alfa ve diger dozdaki EMSA Eritin'e gore daha etkili idi.  Calisma bulgularimiza gore, EMSA Eritin 500 rad doz ile irradyasyon sonrasi homeostaz ve eritropoezi normallestiren koruyucu bir ajan islevi gosterebilecek guclu bir tedavici edici herbal destekleyicidir. Anahtar sozcukler: Antioksidan; kemik iligi; kompleman regulatorler; eritropoez; total vucut irradyasyonu.  This study aims to examine the effect of polyherbal erythropoiesis modulatory and stimulatory agent (EMSA) Eritin administration in modulating the activity of erythropoiesis on TER119+VLA 4+ cells and complement regulators TER 119+CD55+ and TER 119+CD59+ cells in Balb/c mice after total body irradiation (TBI) with a dose of 500 rad.  Eritin was administered orally in mice after exposure to TBI with a dose of 500 rad daily for two weeks with three different doses including a low dose (1.04 mg/g BW) normal dose (3.125 mg/g BW) and high dose (9.375 mg/g BW) Mice were treated with HEMAPO Epoetin alfa twice a week as positive control with a dose of 0.21 mg/g BW. On day 15, the bone marrow was isolated and the number of TER 119+VLA 4+ TER 119+CD55+ and TER 119+CD59+ cells were analyzed using flow cytometry. Examination showed marked reduction in the number of TER 119+VLA 4+ cells in the bone marrow of mice after TBI with dose of 500 rad compared to those of healthy controls. The number of TER 119+VLA 4+ cells in the irradiated mice was markedly increased with EMSA Eritin from low dose to high dose compared to mice with TBI and mice treated with Epoetin alfa.  The highest dose of EMSA Eritin was more effective to promote erythropoiesis than HEMAPO Epoetin alfa and the other dose of EMSA Eritin.  Our study findings suggest that EMSA Eritin is a powerful medicinal herbal supplement which may serve as a protective agent to normalize homeostasis and erythropoiesis after irradiation with a dose of 500 rad.