nils-herrmann/clean_scirepeval huggingface dataset:
Cellular and synaptic adaptations of neural circuits processing skylight polarization in the fly
Venue: Journal of Comparative Physiology A

Specialized ommatidia harboring polarization-sensitive photoreceptors exist in the ‘dorsal rim area’ (DRA) of virtually all insects. Although downstream elements have been described both anatomically and physiologically throughout the optic lobes and the central brain of different species, little is known about their cellular and synaptic adaptations and how these shape their functional role in polarization vision. We have previously shown that in the DRA of Drosophila melanogaster , two distinct types of modality-specific ‘distal medulla’ cell types (Dm-DRA1 and Dm-DRA2) are post-synaptic to long visual fiber photoreceptors R7 and R8, respectively. Here we describe additional neuronal elements in the medulla neuropil that manifest modality-specific differences in the DRA region, including DRA-specific neuronal morphology, as well as differences in the structure of pre- or post-synaptic membranes. Furthermore, we show that certain cell types (medulla tangential cells and octopaminergic neuromodulatory cells) specifically avoid contacts with polarization-sensitive photoreceptors. Finally, while certain transmedullary cells are specifically absent from DRA medulla columns, other subtypes show specific wiring differences while still connecting the DRA to the lobula complex, as has previously been described in larger insects. This hints towards a complex circuit architecture with more than one pathway connecting polarization-sensitive DRA photoreceptors with the central brain.
Neuromodulation of insect motion vision
Venue: Journal of Comparative Physiology A

Insects use vision to choose from a repertoire of flexible behaviors which they perform for survival. Decisions for behavioral plasticity are achieved through the neuromodulation of sensory processes, including motion vision. Here, we briefly review the anatomy of the insect motion vision system. Next, we review the neuromodulatory influences on motion vision. Serotonin modulates peripheral visual processing, whereas octopamine modulates all stages of visual processing tested to date. The physiological and behavioral states that elicit neuromodulation of motion vision include locomotion, changes in internal physiological state such as hunger, and changes in the external environment such as the presence of additional sensory cues. The direction of influence between these states and neuromodulators remains unknown. The influence of neuromodulators on motion vision circuitry has been revealed mostly through pharmacological application, which broadcasts widely with unnatural spatiotemporal dynamics. Thus, insight from this method is limited. Aminergic neurons likely act in local hierarchical fashion rather than globally as a group. As genetic tools advance in Drosophila , future work restricting the experimental focus to subpopulations of modulatory neurons will provide insight into the local functional modifications of visual circuits by interacting neuromodulators.
Optic lobe organization in stomatopod crustacean species possessing different degrees of retinal complexity
Venue: Journal of Comparative Physiology A

Stomatopod crustaceans possess tripartite compound eyes; upper and lower hemispheres are separated by an equatorial midband of several ommatidial rows. The organization of stomatopod retinas is well established, but their optic lobes have been studied less. We used histological staining, immunolabeling, and fluorescent tracer injections to compare optic lobes in two 6-row midband species, Neogonodactylus oerstedii and Pseudosquilla ciliata , to those in two 2-row midband species, Squilla empusa and Alima pacifica . Compared to the 6-row species, we found structural differences in all optic neuropils in both 2-row species. Photoreceptor axons from 2-row midband ommatidia supply two sets of lamina cartridges; however, conspicuous spaces lacking lamina cartridges are observed in locations corresponding to where the cartridges of the upper four ommatidial rows of 6-row species would exist. The tripartite arrangement and enlarged projections containing fibers associated with the two rows of midband ommatidia can be traced throughout the entire optic lobe. However, 2-row species lack some features of medullar and lobular neuropils in 6-row species. Our results support the hypothesis that 2-row midband species are derived from a 6-row ancestor, and suggest specializations in the medulla and lobula found solely in 6-row species are important for color and polarization analysis.
Intracranial pressure in the American Alligator (Alligator mississippiensis): reptilian meninges and orthostatic gradients
Venue: Journal of Comparative Physiology A

The cranial meninges of reptiles differ from the more widely studied mammalian pattern in that the intraventricular and subarachnoid spaces are, at least partially, isolated. This study was undertaken to investigate the bulk flow of cerebrospinal fluid, and the resulting changes in intracranial pressure, in a common reptilian species. Intracranial pressure was measured using ocular ultrasonography and by surgically implanting pressure cannulae into the cranial subarachnoid space. The system was then challenged by: rotating the animal to create orthostatic gradients, perturbation of the vascular system, administration of epinephrine, and cephalic cutaneous heating. Pressure changes determined from the implanted catheters and through quantification of the optic nerve sheath were highly correlated and showed a significant linear relationship with orthostatic gradients. The catheter pressure responses were phasic, with an initial rapid response followed by a much slower response; each phase accounted for roughly half of the total pressure change. No significant relationship was found between intracranial pressure and either heart rate or blood flow. The focal application of heat and the administration of epinephrine both increased intracranial pressure, the latter influence being particularly pronounced.
Differential antipredatory responses in the tuco-tuco (Ctenomys talarum) in relation to endogenous and exogenous changes in glucocorticoids
Venue: Journal of Comparative Physiology A

Glucocorticoids participate in the behavioral and physiological responses generated under stressful circumstances coming from different sources—physical and/or psychological. In mammals, the increases of these hormones are mediated by the activation of the hypothalamic–pituitary–adrenal axis. This response occurs after exposure to novel and unpredictable situations that lead to the loss of homeostasis, for example, a direct encounter with predators or their cues. However, the relationship between the physiological and behavioral responses is still a complex issue in vertebrates. We evaluate the effects of an experimental manipulation of glucocorticoid levels on the generation of the behavioral and physiological response to stress by predation in the subterranean rodent C. talarum. We found that when tuco-tucos encountered predator cues—fur odor, and largely, immobilization—they responded physiologically by secreting cortisol. This response was accompanied by an associated behavioral response. However, when the increase in plasma cortisol originated exogenously by the injection of cortisol, a behavioral response was not observed. Finally, inhibition of glucocorticoids’ synthesis was effective in weakening the behavioral effects produced by immobilization. In conclusion, in tuco-tucos, predator cues act as stress factors that trigger differential increases in plasma cortisol and a behavioral response associated with the appearance of anxiety states.
Acute neuroestrogen blockade attenuates song-induced immediate early gene expression in auditory regions of male and female zebra finches
Venue: Journal of Comparative Physiology A

Neuron-derived estrogens are synthesized by aromatase and act through membrane receptors to modulate neuronal physiology. In many systems, long-lasting hormone treatments can alter sensory-evoked neuronal activation. However, the significance of acute neuroestrogen production is less understood. Both sexes of zebra finches can synthesize estrogens rapidly in the auditory cortex, yet it is unclear how this modulates neuronal cell signaling. We examined whether acute estrogen synthesis blockade attenuates auditory-induced expression of early growth response 1 (Egr-1) in the auditory cortex of both sexes. cAMP response element-binding protein phosphorylation (pCREB) induction by song stimuli and acute estrogen synthesis was also examined. We administered the aromatase inhibitor fadrozole prior to song exposure and measured Egr-1 across several auditory regions. Fadrozole attenuated Egr-1 in the auditory cortex greater in males than females. Females had greater expression and clustering of aromatase cells than males in high vocal center (HVC) shelf. Auditory-induced Egr-1 expression exhibited a large sex difference following fadrozole treatment. We did not observe changes in pCREB expression with song presentation or aromatase blockade. These findings are consistent with the hypothesis that acute neuroestrogen synthesis can drive downstream transcriptional responses in several cortical auditory regions, and that this mechanism is more prominent in males.
Transcriptome analyses provide the first insight into the molecular basis of cold tolerance in Larimichthys polyactis
Venue: Journal of Comparative Physiology B

Larimichthys polyactis is one of the most economically important marine fish species that have become newly cultured in China in recent years. The gene expression changes that L. polyactis experiences in cold toleranceis still unknown, limiting the expansion of its cultivation, fast growth, and high yield. To investigate the molecular mechanism behind L. polyactis’s cold tolerance and to provide a resource for conducting genetic research on L. polyactis, transcriptome sequencing using RNA-seq was performed on individuals that survived cold stress at 4 °C (cold tolerant, CT), and individuals that barely survived 4 °C (cold sensitive, CS), which was considered as the control. A number of 387,607,550 clean reads were obtained from the transcriptomes, and comparative transcriptomic analysis identified 141 differently expressed genes (DEGs), of which 67 were up-regulated and 74 were down-regulated in CT compared to CS under cold stress. Furthermore, ten differently expressed genes were selected from the RNA-Seq analysis to be further validated by real-time PCR. Functional network analysis indicated that L. polyactis adapted to cold stress by employing a series of mechanisms to minimize damages caused by exposure to cold temperatures. The molecular mechanisms identified through RNA-Seq included Extracellular matrix (ECM) receptor interaction, glycerolipid metabolism, regulation of autophagy and focal adhesion pathway as playing vital roles in cold tolerance in L. polyactis. This study may help elucidate how L. polyactis tolerates cold, which is of value for breeding cold-tolerant L. polyactis stocks for cultivation.
Season and sex have different effects on hematology and cytokines in striped hamsters (Cricetulus barabensis)
Venue: Journal of Comparative Physiology B

Animals in the temperate zones face seasonal variations in environments and hence their immune responses change seasonally. In the current study, seasonal changes in hematological parameters and cytokines in striped hamsters (Cricetulus barabensis) were examined to test the winter immunoenhancement hypothesis, which states that immune function tends to increase in fall and winter compared with other seasons. Male and female hamsters were captured from the wild in the fall and winter of 2014 and in the spring and summer of 2015. Maximum body mass in both sexes and relative fatness in female hamsters occurred in the summer, indicating that body condition was the best during this season. All hematological parameters were not different between male and female hamsters, and were also not affected by the interaction of season and sex except neutrophil granulocytes (GRAN). Red blood cells (RBC) and haematocrit (PCV) were higher in the fall and winter, and hemoglobin concentration (HGB) was the highest in winter in hamsters compared with the spring and summer, implying that their oxygen-carrying capacity and oxygen affinity of the blood increased during these seasons. Compared with other seasons, the number of white blood cells (WBC) was higher in winter than in summer, intermediate granulocytes (MID), the percent of MID (MID%), GRAN and the percent of GRAN (GRAN%) were the highest in winter, which all supported the winter immunoenhancement hypothesis. However, the count of lymphocytes (LYMF) was the highest in spring, being inconsistent with this hypothesis. IL-2 levels, but not TNF-α, were influenced by seasons, sex and their interaction in hamsters. Regardless of sex, IL-4 titres were higher in spring and summer than in fall and winter in hamsters. INF-γ titres in male hamsters did not differ between the spring and summer, while its titres in female hamsters was lower in spring in contrast with winter and summer. Higher IL-2 and IL-4 levels during the breeding seasons might be crucial in controlling the increased possibilities of infections in these seasons. In summary, season and sex had disparate effects on different hematological profiles and the levels of cytokines in hamsters.
Autophagy and Akt-mTOR signaling display periodic oscillations during torpor-arousal cycles in oxidative skeletal muscle of Daurian ground squirrels (Spermophilus dauricus)
Venue: Journal of Comparative Physiology B

Whether hibernation accelerates or suppresses autophagy is still unknown. In the current study, we examined changes in autophagy in oxidative soleus (SOL) muscle in summer active (SA), pre-hibernation (PRE), torpor (TOR), interbout arousal (IBA), and post-hibernation groups of Daurian ground squirrels (Spermophilus dauricus). Here, the SOL muscle showed no significant atrophy during hibernation in regard to muscle wet weight, fiber cross-sectional area, or MuRF1 protein level. Autophagy-related proteins beclin1 and Atg7 increased significantly, whereas LC3-II decreased significantly in the PRE group compared with the SA group. However, neither the expression nor activity of cathepsin L showed any differences between the SA and PRE groups. In addition, beclin1, LC3-II, and the LC3-II/LC3-I ratio increased, p62 decreased, LC3 puncta increased, p62 puncta decreased, and cathepsin L activity increased in the TOR group compared with the PRE group. In contrast, beclin1, LC3-II, and the LC3-II/LC3-I ratio decreased, p62 increased, LC3 puncta decreased, p62 puncta increased, and cathepsin L activity declined in the IBA group compared with the TOR group. Moreover, the phosphorylation of Akt (Ser473) and mTOR (Ser2448) changed significantly during hibernation and showed an inverse relationship with autophagy changes. In conclusion, autophagy proteins displayed periodic oscillation in the torpor-arousal cycle, which may be advantageous in maintaining SOL muscle mass during the entire hibernation period. Furthermore, the Akt-mTOR signaling was decreased in TOR and increased in IBA group in the SOL muscle of Daurian ground squirrels during hibernation.
From phenoloxidase to fecundity: food availability does not influence the costs of oxidative challenge in a wing-dimorphic cricket
Venue: Journal of Comparative Physiology B

Stressed animals often struggle to maintain optimal investment into a number of fitness-related traits, which can result in some traits being more adversely affected than others. Variation in stress-related costs may also depend on the environment—costs can be facultative and only occur when resources are limited, or they may be obligate and occur regardless of resource availability. Dynamics of oxidative stress may be important in life-history evolution given their role in a range of biological processes—from reproduction to immunity to locomotion. Thus, we examined how resource (food) availability influences the costs of oxidative challenge to fitness-related traits spanning several levels of biological organization. We manipulated food availability and oxidative status in females of the wing-dimorphic sand field cricket ( Gryllus firmus ) during early adulthood. We then determined investment into several traits: reproduction (ovary mass), soma (body mass and flight musculature), and immune function (total phenoloxidase activity). Oxidative challenge (paraquat exposure) obligated costs to somatic tissue and a parameter of immune function regardless of food availability, but it did not affect reproduction. We show that the costs of oxidative challenge are trait-specific, but we did not detect a facultative (food-dependent) cost of oxidative challenge to any trait measured. Although the dynamics of oxidative stress are complex, our study is an important step toward a more complete understanding of the roles that resource availability and redox systems play in mediating life histories.
The diversity of lobula plate tangential cells (LPTCs) in the Drosophila motion vision system
Venue: Journal of Comparative Physiology A

To navigate through the environment, animals rely on visual feedback to control their movements relative to their surroundings. In dipteran flies, visual feedback is provided by the wide-field motion-sensitive neurons in the visual system called lobula plate tangential cells (LPTCs). Understanding the role of LPTCs in fly behaviors can address many fundamental questions on how sensory circuits guide behaviors. The blowfly was estimated to have ~ 60 LPTCs, but only a few have been identified in Drosophila. We conducted a Gal4 driver screen and identified five LPTC subtypes in Drosophila, based on their morphological characteristics: LPTCs have large arborizations in the lobula plate and project to the central brain. We compared their morphologies to the blowfly LPTCs and named them after the most similar blowfly cells: CH, H1, H2, FD1 and FD3, and V1. We further characterized their pre- and post-synaptic organizations, as well as their neurotransmitter profiles. These anatomical features largely agree with the anatomy and function of their likely blowfly counterparts. Nevertheless, several anatomical details indicate the Drosophila LPTCs may have more complex functions. Our characterization of these five LPTCs in Drosophila will facilitate further functional studies to understand their roles in the visual circuits that instruct fly behaviors.
Can´t beat the heat? Importance of cardiac control and coronary perfusion for heat tolerance in rainbow trout
Venue: Journal of Comparative Physiology B

Coronary perfusion and cardiac autonomic regulation may benefit myocardial oxygen delivery and thermal performance of the teleost heart, and thus influence whole animal heat tolerance. Yet, no study has examined how coronary perfusion affects cardiac output during warming in vivo. Moreover, while β-adrenergic stimulation could protect cardiac contractility, and cholinergic decrease in heart rate may enhance myocardial oxygen diffusion at critically high temperatures, previous studies in rainbow trout (Oncorhynchus mykiss) using pharmacological antagonists to block cholinergic and β-adrenergic regulation showed contradictory results with regard to cardiac performance and heat tolerance. This could reflect intra-specific differences in the extent to which altered coronary perfusion buffered potential negative effects of the pharmacological blockade. Here, we first tested how cardiac performance and the critical thermal maximum (CTmax) were affected following a coronary ligation. We then assessed how these performances were influenced by pharmacological cholinergic or β-adrenergic blockade, hypothesising that the effects of the pharmacological treatment would be more pronounced in coronary ligated trout compared to trout with intact coronaries. Coronary blockade reduced CTmax by 1.5 °C, constrained stroke volume and cardiac output across temperatures, led to earlier cardiac failure and was associated with reduced blood oxygen-carrying capacity. Nonetheless, CTmax and the temperatures for cardiac failure were not affected by autonomic blockade. Collectively, our data show that coronary perfusion improves heat tolerance and cardiac performance in trout, while evidence for beneficial effects of altered cardiac autonomic tone during warming remains inconclusive.
A new view of sexual differentiation of mammalian brain
Venue: Journal of Comparative Physiology A

Establishment of enduring sex differences in brain and behavior occurs during pre- or perinatal development, depending on species. For over 50 years the focus has been on gonadal steroid production by male fetuses and the impact on developing brain. An increasing awareness of the importance of sex chromosome complement has broadened the focus but identifying specific roles in development has yet to be achieved. Recent emphasis on transcriptomics has revealed myriad and unexpected differences in gene expression in specific regions of male and female brains which may produce sex differences, serve a compensatory role or provide latent sex differences revealed only in response to challenge. More surprising, however, has been the consistent observation of a central role for inflammatory signaling molecules and immune cells in masculinization of brain and behavior. The signal transduction pathways and specific immune cells vary by brain region, as does the neuroanatomical substrate subject to differentiation, reflecting substantial complexity emerging from what may be a common origin, the maternal immune system. A working hypothesis integrating these various ideas is proposed.
Brain structure differences between solitary and social wasp species are independent of body size allometry
Venue: Journal of Comparative Physiology A

Evolutionary transitions in social behavior are often associated with changes in species’ brain architecture. A recent comparative analysis showed that the structure of brains of wasps in the family Vespidae differed between solitary and social species: the mushroom bodies, a major integrative brain region, were larger relative to brain size in the solitary species. However, the earlier study did not account for body size effects, and species’ relative mushroom body size increases with body size in social Vespidae. Here we extend the previous analysis by measuring the effects of body size variation on brain structure differences between social and solitary vespid wasps. We asked whether total brain volume was greater relative to body size in the solitary species, and whether relative mushroom body size was greater in solitary species, after accounting for body size effects. Both total brain volume and relative mushroom body volume were significantly greater in the solitary species after accounting for body size differences. Therefore, body size allometry did not explain the solitary versus social species differences in brain structure. The evolutionary transition from solitary to social behavior in Vespidae was accompanied by decreases in total brain size and in relative mushroom body size.