nils-herrmann/clean_scirepeval huggingface dataset:
Evaluation of the kinetic energy of the torso by magneto-inertial measurement unit during the sit-to-stand movement.
Venue: Journal of biomechanics

Sit-to-stand tests are used in geriatrics as a qualitative issue in order to evaluate motor control and stability. In terms of measured indicators, it is traditionally the duration of the task that is reported, however it appears that the use of the kinetic energy as a new quantitative criterion allows getting a better understanding of musculoskeletal deficits of elderly subjects. The aim of this study was to determine the feasibility to obtain the measure of kinetic energy using magneto-inertial measurement units (MIMU) during sit-to-stand movements at various paces. 26 healthy subjects contributed to this investigation. Measured results were compared to a marker-based motion capture using the correlation coefficient and the normalized root mean square error (nRMSE). nRMSE were below 10% and correlation coefficients were over 0.97. In addition, errors on the mean kinetic energy were also investigated using Bland-Altman 95% limits of agreement (0.63 J-0.77 J), RMSE (0.29 J-0.38 J) and correlation coefficient (0.96-0.98). The results obtained highlighted that the method based on MIMU data could be an alternative to optoelectronic data acquisition to assess the kinetic energy of the torso during the sit-to-stand test, suggesting this method as being a promising alternative to determine kinetic energy during the sit-to-stand movement.
Intraoperative and biomechanical studies of human vastus lateralis and vastus medialis sarcomere length operating range.
Venue: Journal of biomechanics

The vast majority of musculoskeletal models are not validated against primary experimental data. Conversely, most human experimental measurements are not explained theoretically using models to provide a mechanistic understanding of experimental results. Here we present a study with both primary human data and primary modeling data. Intraoperative sarcomere length was measured on the human vastus lateralis (VL) and vastus medialis (VM) muscles (n = 8) by laser diffraction. These data were compared to a biomechanical model based on muscle architecture and moment arms obtained independently from cadaveric specimens (n = 9). Measured VL sarcomere length ranged from about 3.2 µm with the knee flexed to 45° to 3.8 µm with the knee flexed to 90°. These values were remarkably close to theoretical predictions. Measured VM sarcomere length ranged from 3.6 µm with the knee flexed to 45° to 4.1 µm with the knee flexed to 90°. These values were dramatically longer than theoretical predictions. Our measured sarcomere length values suggest that human vasti may have differing functions with regard to knee extension and patellar stabilization. This report underscores the importance of validating experimental data to theoretical models and vice versa.
Effect on the mechanical properties of type I collagen of intra-molecular lysine-arginine derived advanced glycation end-product cross-linking
Venue: Journal of biomechanics

Non-enzymatic advanced glycation end product (AGE) cross-linking of collagen molecules has been hypothesised to result in significant changes to the mechanical properties of the connective tissues within the body, potentially resulting in a number of age related diseases. We have investigated the effect of two of these cross-links, glucosepane and DOGDIC, on the tensile and lateral moduli of the collagen molecule through the use of a steered molecular dynamics approach, using previously identified preferential formation sites for intra-molecular cross-links. Our results show that the presence of intra-molecular AGE cross-links increases the tensile and lateral Young’s moduli in the low strain domain by between 3.0–8.5% and 2.9–60.3% respectively, with little effect exhibited at higher strains.
Biomechanical measurement and analysis of colchicine-induced effects on cells by nanoindentation using an atomic force microscope.
Venue: Journal of biomechanics

Colchicine is a drug commonly used for the treatment of gout, however, patients may sometimes encounter side-effects induced by taking colchicine, such as nausea, vomiting, diarrhea and kidney failure. In this regard, it is imperative to investigate the mechanism effects of colchicine on biological cells. In this paper, we present a method for the detection of mechanical properties of nephrocytes (VERO cells), hepatocytes (HL-7702 cells) and hepatoma cells (SMCC-7721 cells) in culture by atomic force microscope (AFM) to analyze the 0.1 μg/mL colchicine-induced effects on the nanoscale for two, four and six hours. Compared to the corresponding control cells, the biomechanical properties of the VERO and SMCC-7721 cells changed significantly and the HL-7702 cells did not considerably change after the treatment when considering the same time period. Based on biomechanical property analyses, the colchicine solution made the VERO and SMCC-7721 cells harder. We conclude that it is possible to reduce the division rate of the VERO cells and inhibit the metastasis of the SMCC-7721 cells. The method described here can be applied to study biomechanics of many other types of cells with different drugs. Therefore, this work provides an accurate and rapid method for drug screening and mechanical analysis of cells in medical research.
Biomechanical consequences of running with deep core muscle weakness.
Venue: Journal of biomechanics

The deep core muscles are often neglected or improperly trained in athletes. Improper function of this musculature may lead to abnormal spinal loading, muscle strain, or injury to spinal structures, all of which have been associated with increased low back pain (LBP) risk. The purpose of this study was to identify potential strategies used to compensate for weakness of the deep core musculature during running and to identify accompanying changes in compressive and shear spinal loads. Kinematically-driven simulations of overground running were created for eight healthy young adults in OpenSim at increasing levels of deep core muscle weakness. The deep core muscles (multifidus, quadratus lumborum, psoas, and deep fascicles of the erector spinae) were weakened individually and together. The superficial longissimus thoracis was a significant compensator for 4 out of 5 weakness conditions (p < 0.05). The deep erector spinae required the largest compensations when weakened individually (up to a 45 ± 10% increase in compensating muscle force production, p = 0.004), revealing it may contribute most to controlling running kinematics. With complete deep core muscle weakness, peak anterior shear loading increased on all lumbar vertebrae (up to 19%, p = 0.001). Additionally, compressive spinal loading increased on the upper lumbar vertebrae (up to 15%, p = 0.007) and decreased on the lower lumbar vertebrae (up to 8%, p = 0.008). Muscular compensations may increase risk of muscular fatigue or injury and increased spinal loading over numerous gait cycles may result in damage to spinal structures. Therefore, insufficient strength of the deep core musculature may increase a runner's risk of developing LBP.
Novel image analysis methods for quantification of in situ 3-D tendon cell and matrix strain.
Venue: Journal of biomechanics

Macroscopic tendon loads modulate the cellular microenvironment leading to biological outcomes such as degeneration or repair. Previous studies have shown that damage accumulation and the phases of tendon healing are marked by significant changes in the extracellular matrix, but it remains unknown how mechanical forces of the extracellular matrix are translated to mechanotransduction pathways that ultimately drive the biological response. Our overarching hypothesis is that the unique relationship between extracellular matrix strain and cell deformation will dictate biological outcomes, prompting the need for quantitative methods to characterize the local strain environment. While 2-D methods have successfully calculated matrix strain and cell deformation, 3-D methods are necessary to capture the increased complexity that can arise due to high levels of anisotropy and out-of-plane motion, particularly in the disorganized, highly cellular, injured state. In this study, we validated the use of digital volume correlation methods to quantify 3-D matrix strain using images of naïve tendon cells, the collagen fiber matrix, and injured tendon cells. Additionally, naïve tendon cell images were used to develop novel methods for 3-D cell deformation and 3-D cell-matrix strain, which is defined as a quantitative measure of the relationship between matrix strain and cell deformation. The results support that these methods can be used to detect strains with high accuracy and can be further extended to an in vivo setting for observing temporal changes in cell and matrix mechanics during degeneration and healing.
Effects of mechanical stretch on the functions of BK and L-type Ca2+ channels in vascular smooth muscle cells.
Venue: Journal of biomechanics

It is well recognized that pathologically increased mechanical stretch plays a critical role in vascular remodeling during hypertension. However, how the stretch modulates the functions of ion channels of vascular smooth muscle cells (VSMCs) remains to be elucidated. Here, we demonstrated the effects of mechanical stretch on the activity of large conductance calcium, voltage-activated potassium (BK) and L-type Ca2+ channels. In comparison with 5% stretch (physiological), 15% stretch (pathological) upregulated the current density of L-type Ca2+ and BK channels as well as the frequency and amplitude of calcium oscillation in VSMCs. 15% stretch also increased the open probability and mean open time of the BK channel compared with 5% stretch. BK and L-type Ca2+ channels participated in the mechanical stretch-modulated calcium oscillation. Our results suggested that during hypertension, pathological stretch altered the activity of BK and L-type Ca2+ channels and manipulated the calcium oscillation of VSMCs.
Thoracolumbar spine loading associated with kinematics of the young and the elderly during activities of daily living.
Venue: Journal of biomechanics

Excessive mechanical loading of the spine is a critical factor in vertebral fracture initiation. Most vertebral fractures develop spontaneously or due to mild trauma, as physiological loads during activities of daily living might exceed the failure load of osteoporotic vertebra. Spinal loading patterns are affected by vertebral kinematics, which differ between elderly and young individuals. In this study, the effects of age-related changes in spine kinematics on thoracolumbar spinal segmental loading during dynamic activities of daily living were investigated using combined experimental and modeling approach. Forty-four healthy volunteers were recruited into two age groups: young (N = 23, age = 27.1 ± 3.8) and elderly (N = 21, age = 70.1 ± 3.9). The spinal curvature was assessed with a skin-surface device and the kinematics of the spine and lower extremities were recorded during daily living tasks (flexion-extension and stand-sit-stand) with a motion capture system. The obtained data were used as input for a musculoskeletal model with a detailed thoracolumbar spine representation. To isolate the effect of kinematics on predicted loads, other model properties were kept constant. Inverse dynamics simulations were performed in the AnyBody Modeling System to estimate corresponding spinal loads. The maximum compressive loads predicted for the elderly motion patterns were lower than those of the young for L2/L3 and L3/L4 lumbar levels during flexion and for upper thoracic levels during stand-to-sit (T1/T2-T8/T9) and sit-to-stand (T3/T4-T6/T7). However, the maximum loads predicted for the lower thoracic levels (T9/T10-L1/L2), a common site of vertebral fractures, were similar compared to the young. Nevertheless, these loads acting on the vertebrae of reduced bone quality might contribute to a higher fracture risk for the elderly.
Manipulating post-stroke gait: Exploiting aberrant kinematics.
Venue: Journal of biomechanics

Post-stroke individuals often exhibit abnormal kinematics, including increased pelvic obliquity and hip abduction coupled with reduced knee flexion. Prior examinations suggest these behaviors are expressions of abnormal cross-planar coupling of muscle activity. However, few studies have detailed the impact of gait-retraining paradigms on three-dimensional joint kinematics. In this study, a cross-tilt walking surface was examined as a novel gait-retraining construct. We hypothesized that relative to baseline walking kinematics, exposure to cross-tilt would generate significant changes in subsequent flat-walking joint kinematics during affected limb swing. Twelve post-stroke participants walked on a motorized treadmill platform during a flat-walking condition and during a 10-degree cross-tilt with affected limb up-slope, increasing toe clearance demand. Individuals completed 15 min of cross-tilt walking with intermittent flat-walking catch trials and a final washout period (5 min). For flat-walking conditions, we examined changes in pelvic obliquity, hip abduction/adduction and knee flexion kinematics at the spatiotemporal events of swing initiation and toe-off, and the kinematic event of maximum angle during swing. Pelvic obliquity significantly reduced at swing initiation and maximum obliquity in the final catch trial and late washout. Knee flexion significantly increased at swing initiation, toe-off, and maximum flexion across catch trials and late washout. Hip abduction/adduction was not significantly influenced following cross-tilt walking. Significant decrease in the rectus femoris and medial hamstrings muscle activity across catch trials and late washout was observed. Exploiting the abnormal features of post-stroke gait during retraining yielded desirable changes in muscular and kinematic patterns post-training.
Thigh-calf contact parameters for six high knee flexion postures: Onset, maximum angle, total force, contact area, and center of force.
Venue: Journal of biomechanics

In high knee flexion, contact between the posterior thigh and calf is expected to decrease forces on tibiofemoral contact surfaces, therefore, thigh-calf contact needs to be thoroughly characterized to model its effect. This study measured knee angles and intersegmental contact parameters in fifty-eight young healthy participants for six common high flexion postures using motion tracking and a pressure sensor attached to the right thigh. Additionally, we introduced and assessed the reliability of a method for reducing noise in pressure sensor output. Five repetitions of two squatting, two kneeling, and two unilateral kneeling movements were completed. Interactions of posture by sex occurred for thigh-calf and heel-gluteal center of force, and thigh-calf contact area. Center of force in thigh-calf regions was farther from the knee joint center in females, compared to males, during unilateral kneeling (82 and 67 mm respectively) with an inverted relationship in the heel-gluteal region (331 and 345 mm respectively), although caution is advised when generalizing these findings from a young, relatively fit sample to a population level. Contact area was larger in females when compared to males (mean of 155.61 and 137.33 cm2 across postures). A posture main effect was observed in contact force and sex main effects were present in onset and max angle. Males had earlier onset (121.0°) and lower max angle (147.4°) with onset and max angles having a range between movements of 8° and 3° respectively. There was a substantial total force difference of 139 N between the largest and smallest activity means. Force parameters measured in this study suggest that knee joint contact models need to incorporate activity-specific parameters when estimating loading.
Coupled motions in human and porcine thoracic and lumbar spines.
Venue: Journal of biomechanics

Coupled motions, i.e., motions along axes other than the loaded axis, have been reported to occur in the human spine, and are likely to be influenced by inclined local axes due to the sagittal plane spine curvature. Furthermore, the role of facet joints in such motions is as yet unclear. Therefore, this study aimed at assessing coupled motions in multiple spine sections in vitro, before and after removal of posterior elements. Six elderly human and 6 young porcine spines were sectioned in four segments (high thoracic, mid thoracic, low thoracic and lumbar), each consisting of four vertebrae and three intervertebral discs. Segments were loaded along each of the three axes, and three-dimensional rotations of the middle segment were quantified. Subsequently, posterior elements were removed and the protocol was repeated. To avoid mixed loading between Axial Rotation (AR) and Lateral Bending (LB), in contrast to other studies, local axes at the vertebrae were defined as aligned with the loading device prior to each load application. Expressed as a percentage of motion in the loaded direction, coupled motions were on average larger in human (22.7%, SD = 2.2%) than in porcine (11.9%, SD = 1.2%) spines (p < .001). Largest coupled motions were obtained in AR loading of the lumbar spine segments, with mean magnitudes averaged over coupling axes for human L2-L3 joints of 48.9% (SD = 13.2%), including somewhat more LB (56.4%, SD = 18.6) than FE (41.4%, SD = 14.1%) coupling. For porcine L3-L4 joints average coupling in AR loading was 29.3% (SD = 8.2%). In human segments removal of posterior elements only had substantial effects in the lumbar spine segments, where posterior element removal decreased coupled motion during AR loading, averaged over LB and FE coupling, from 48.9% (SD = 13.2%) to 27.7% (SD = 6.1%), mainly through increased motion in the loaded direction. The present results indicate that coupled motions were largest in the lumbar spine. In human spines, posterior elements only contributed to coupled motions in lumbar axial rotation loading.
Muscle tension increases impact force but decreases energy absorption and pain during visco-elastic impacts to human thighs.
Venue: Journal of biomechanics

Despite uncertainty of its exact role, muscle tension has shown an ability to alter human biomechanical response and may have the ability to reduce impact injury severity. The aim of this study was to examine the effects of muscle tension on human impact response in terms of force and energy absorbed and the subjects' perceptions of pain. Seven male martial artists had a 3.9 kg medicine ball dropped vertically from seven different heights, 1.0-1.6 m in equal increments, onto their right thigh. Subjects were instructed to either relax or tense the quadriceps via knee extension (≥60% MVC) prior to each impact. F-scan pressure insoles sampling at 500 Hz recorded impact force and video was recorded at 1000 Hz to determine energy loss from the medicine ball during impact. Across all impacts force was 11% higher, energy absorption was 15% lower and time to peak force was 11% lower whilst perceived impact intensity was significantly lower when tensed. Whether muscle is tensed or not had a significant and meaningful effect on perceived discomfort. However, it did not relate to impact force between conditions and so tensing may alter localised injury risk during human on human type impacts.
Tendon displacements during voluntary and involuntary finger movements.
Venue: Journal of biomechanics

In the human hand, independent movement control of individual fingers is limited. One potential cause for this is mechanical connections between the tendons and muscle bellies corresponding to the different fingers. The aim of this study was to determine the tendon displacement of the flexor digitorum superficialis (FDS) of both the instructed and the neighboring, non-instructed fingers during single finger flexion movements. In nine healthy subjects (age 22-29 years), instructed and non-instructed FDS finger tendon displacement of the index, middle and ring finger was measured using 2D ultrasound analyzed with speckle tracking software in two conditions: active flexion of all finger joints with all fingers free to move and active flexion while the non-instructed fingers were restricted. Our results of the free movement protocol showed an average tendon displacement of 27 mm for index finger flexion, 21 mm for middle finger flexion and 17 mm for ring finger flexion. Displacements of the non-instructed finger tendons (≈12 mm) were higher than expected based of the amount of non-instructed finger movement. In the restricted protocol, we found that, despite minimal joint movements, substantial non-instructed finger tendon displacement (≈9 mm) was still observed, which was interpreted as a result of tendon strain. When this strain component was subtracted from the tendon displacement of the non-instructed fingers during the free movement condition, the relationship between finger movement and tendon displacement of the instructed and non-instructed finger became comparable. Thus, when studying non-instructed finger tendon displacement it is important to take tendon strain into consideration.
Estimation of spinal joint centers from external back profile and anatomical landmarks.
Venue: Journal of biomechanics

Defining a subject-specific model of the human body is required for motion analysis in many fields, such as in ergonomics and clinical applications. However, locating internal joint centers from external characteristics of the body still remains a challenging issue, in particular for the spine. Current methods mostly require a set of rarely accessible (3D back or trunk surface) or operator dependent inputs (large number of palpated landmarks and landmarks-based anthropometrics). Therefore, there is a need to provide an alternative way to estimate joint centers only using a limited number of easily palpable landmarks and the external back profile. Two methods were proposed to predict the spinal joint centers: one using only 6 anatomical landmarks (ALs) (2 PSIS, T8, C7, IJ and PX) and one using both 6 ALs and the external back profile. Regressions were established using the X-ray based 3D reconstructions of 80 subjects and evaluated on 13 additional subjects of variable anthropometry. The predicted location of joint centers showed an average error 9.7 mm (±5.0) in the sagittal plane for all joints when using the external back profile. Similar results were obtained without using the external back profile, 9.5 mm (±5.0). Compared to other existing methods, the proposed methods offered a more accurate prediction with a smaller number of palpated points. Additional methods have to be developed for considering postures other than standing, such as a sitting position.
Dental functional morphology predicts the scaling of chewing rate in mammals.
Venue: Journal of biomechanics

How food intake and mastication scale to satisfy the metabolic needs of mammals has been the subject of considerable scientific debate. Existing theory suggests that the negative allometric scaling of metabolic rate with body mass is compensated by a matching allometric scaling of the chewing rate. Why empirical studies have found that the scaling coefficients of the chewing rate seem to be systematically smaller than expected from theory remains unknown. Here we explain this imparity by decoupling the functional surface area of teeth from overall surface area. The functional surface area is relatively reduced in forms emphasizing linear edges (e.g., lophodont) compared with forms lacking linear structures (e.g., bunodont). In forms with reduced relative functional surface, the deficit in food processed per chew appears to be compensated for by increased chewing rate, such that the metabolic requirements are met. This compensation accounts for the apparent difference between theoretically predicted and observed scaling of chewing rates. We suggest that this reflects adaptive functional evolution to plant foods with different fracture properties and extend the theory to incorporate differences in functional morphology.