Ayreon Universe incluirá a Floor Jansen y Marco Hietala (Nightwish), Russell Allen (Symphony X), Damian Wilson (Threshold), Hansi Kürsch (Blind Guardian), Tommy Karevik (Kamelot), Anneke van Giersbergen (The Gentle Storm), Jonas Renkse (Katatonia), Mike Mills (Toehider), Marcela Bovio (Stream of Passion), Irene Jansen (Ayreon), Robert Soeterboek (Star One), Edward Reekers (Kayak), Jan van Feggelen (Ayreon), Magali Luyten (Nightmare) y Lisette van den Berg (Scarlet Stories).