tokens: Design and patients DNA from 84 subjects , including 26 subjects with sporadic PHP1b , 27 affected subjects and 17 unaffected and/or obligate gene carriers from 12 PHP1b families , 11 healthy individuals , and 3 subjects with PHP1a was subjected to quantitative pyrosequencing of GNAS differentially methylated regions ( DMRs ) , microarray analysis , and microsatellite haplotype analysis .