natgillin/translations-raw huggingface dataset:
Note: Dry casein isolates should be kept at –20 °C.
Poliakrilamidni gel se lahko razreže v manjše kose, kar je priporočljivo zlasti, kadar je vzorcev malo ali če uporabljate avtomatsko elektroforezno napravo (dva gela, velikost 4,5 × 5 cm).
Plasmin cleavage of β-caseins to intensify γ-caseins
Izoelektrično fokusiranje
Disperse 25 mg of isolated caseins (6.1.1) in 0,5 ml ammonium carbonate buffer (4.7.1) and homogenize for 20 minutes by e.g. using ultrasonic treatment.
Nastavite termostat za hlajenje na 12 °C.
Heat to 40 °C and add 10 μl plasmin (4.7.2), mix and incubate for one hour at 40 °C with continuous shaking.
S kerozinom zbrišite hrbtno stran nosilne folije in kapnite nekaj kapljic kerozina (4.2) na sredo hladilnega bloka.
To inhibit the enzyme add 20 μl ε-aminoproic acid solution (4.7.3), then add 200 mg of solid urea and 2 mg of dithiothreitol.
Nato po njem povaljajte gelov sendvič z nosilno stranjo navzdol in pazite, da ne nastanejo mehurčki.
Note: To obtain more symmetry in the focused casein bands it is advisable to freeze-dry the solution after adding the ε-aminocaproic acid and then dissolving the residues in 0,5 ml protein dissolving buffer (4.6).
Obrišite ves odvečni kerozin in odstranite pokrivno folijo.
Preparation of the urea containing polyacrylamide gels
Prepojite elektrodna trakova z elektrodnima raztopinama (4.3, 4.4), odrežite ju v dolžini gela in postavite v pripravljene položaje (razdalja elektrod 9,5 cm).
With the aid of a few drops of water roll the gel carrier sheet (5.2) onto a glass plate (5.1), removing any extraneous water with paper towel or tissue.
Pogoji za izoelektrično fokusiranje:
Roll the cover sheet (5.3) with spacers (0,25 mm) onto another glass plate in the same way.
Velikost gela 265 × 125 × 0,25 mm
Lay the plate horizontally on a levelling table.
Korak
Add 10 μl Temed (4.1.3.1) to the prepared and de-aerated gel solution (4.1.2), stir and add 10 μl PER-solution (4.1.3.2), mix thoroughly and immediately pour out evenly onto the centre of the cover sheet.
Napetost
Place one edge of the gel carrier plate (sheet side down) on the cover sheet plate and lower it slowly so that a gel film forms between the sheets and spreads out regularly and free of bubbles (Figure 3).
Tok
Carefully lower the gel carrier plate completely using a thin spatula and place three more glass plates on top of it to act as weights.
Moč
After polymerization is complete (about 60 minutes) remove the gel polymerized onto the gel carrier sheet along with the cover sheet by tipping the glass plates.
Volt ure
Clean the reverse of the carrier sheet carefully to remove gel residues and urea.
Predhodno fokusiranje
Weld the gel sandwich into a film tube and store in a refrigerator (maximum six weeks).
največ
Note: The cover sheet with the spacers can be re-used.
Vzorčno fokusiranje
The polyacrylamide gel can be cut to smaller sizes, recommended when there are few samples or if an automatic electrophoresis device is used (two gels, size 4,5 × 5 cm).
Končno fokusiranje
lsoelectric focusing
Opomba: Če sta debelina ali širina gela spremenjeni, se morata ustrezno prilagoditi vrednosti za tok in moč (npr. podvojite vrednosti za električni tok in moč, če se uporabi gel 265 × 125 × 0,5 mm).
Set the cooling thermostat to 12 °C.
Primer programa napetosti za avtomatsko elektroforezno napravo (2 gela 5,0 × 4,5 cm), elektrodi brez trakov, ki se neposredno naneseta na gel
Then roll the gel sandwich, carrier side down, onto it, taking care to avoid bubbles.
Fokusiranje
Wipe off any excess kerosene and remove the cover sheet.
V koraku 2 postavite trak za nanos vzorca na 0 Vh.
Soak the electrode strips with the electrode solutions (4.3, 4.4), cut to gel length and place in the positions provided (distance of electrodes 9,5 cm).
V koraku 2 odstranite trak za nanos vzorca pri 30 Vh.
Conditions for isoelectric focusing:
Proteinsko obarvanje
Gel size 265 × 125 × 0,25 mm
Proteinsko fiksiranje
Step
Po izključitvi elektrike takoj odstranite elektrodna trakova in dajte gel v posodo za obarvanje/razbarvanje, napolnjeno z 200 ml fiksirja (4.9); pustite 15 minut in neprestano stresajte.
Voltage
Umivanje in obarvanje gelske plošče
Current
Temeljito odtočite fiksir in dvakrat vsakič po 30 sekund spirajte gelsko ploščo s 100 ml raztopine za razbarvanje (4.10).
Power
Odlijte raztopino za razbarvanje in dopolnite posodo z 250 ml raztopine za obarvanje (4.11.3); počakajte 45 minut in z rahlim tresenjem omogočite, da se obarva.
Volt-hours
Razbarvanje gelske plošče
Pre-focusing
Odlijte raztopino za obarvanje, dvakrat sperite gelsko ploščo, pri čemer vsakič uporabite 100 ml raztopine za razbarvanje (4.10), nato 15 minut tresite z 200 ml raztopine za razbarvanje in najmanj dvakrat ali trikrat ponovite korak razbarvanja, dokler ozadje ne postane čisto in brezbarvno.
maximum
Nato splaknite gelsko ploščo z destilirano vodo (2 × 2 minuti) in sušite na zraku (2 do 3 ure) ali s sušilnikom za lase (10–15 minut).
constant 4
Opomba 1: Fiksiranje, pomivanje, obarvanje in razbarvanje opravite pri temperaturi 20 °C.
Sample focusing
Ne uporabljajte visokih temperatur.
Final focusing
Opomba 2: Če raje uporabljate bolj občutljivo obarvanje s srebrom (npr. oprema za obarvanje s srebrom, protein, Pharmacia Biotech, št. kode 17–1150–01), morate plazminsko obdelane kazeinske vzorce razredčiti do 5 mg/ml.
Note: If thickness or width of the gels are changed, the values for current and power have to be suitably adjusted (e.g. double the values for electric current and power if a 265 × 125 × 0,5 mm gel is used).
OCENJEVANJE
Example of a voltage programme for an automatic electrophoresis device (2 gels of 5,0 × 4,5 cm), electrodes without strips applied directly to the gel
Ocenjevanje se izvaja s primerjavo proteinskih profilov neznanega vzorca z referenčnimi standardi na istem gelu.
Temp.
Zaznavanje kravjega mleka v sirih iz ovčjega, kozjega in bivoličjega mleka in mešanic ovčjega, kozjega in bivoličjega mleka se izvaja prek y3- in y2-kazeinov, katerih izoelektrične točke so v razponu med pH 6,5 in pH 7,5 (slike 4a, 4b in 5).
Focusing
Meja zaznavnosti je manj kot 0,5 %.
Place sample applicator in step 2 at 0 Vh.
Vizualna ocena
Remove sample applicator in step 2 at 30 Vh.
Za vizualno ocenitev količine mleka goveda je priporočljivo, da se koncentracije vzorcev in standardov prilagodijo za pridobitev iste stopnje intenzitete ovčjih, kozjih in/ali bivoličjih γ2- in γ3-kazeinov (glej „γ2 E,G,B“ in „γ3 E,G,B“ na slikah 4a, 4b in 5).
Protein staining
Po tem se lahko neposredno presodi količina mleka goveda (manj kot, enako ali več kot 1 %) v neznanem vzorcu s primerjavo intenzitete kravjih γ3- in γ2-kazeinov (glej „γ3 C“ in „γ2 C“ na slikah 4a, 4b in 5) s tistimi od 0 % in 1 % referenčnih standardov (ovca, koza) ali laboratorijskimi vmesnimi standardi (bivolica).
Protein fixation
Denzitometrijska ocena
Remove the electrode strips immediately after turning off the power and put the gel immediately into a staining/destaining dish filled with 200 ml fixative (4.9); leave for 15 minutes, shaking continuously.
Za določitev razmerja površin pod vrhovi za goveja, ovčja, kozja in/ali bivoličja kazeina γ2 in γ3 (glej sliko 5) uporabite denzitometrijo (5.19), če je na voljo.
Washing and staining the gel plate
Primerjajte to vrednost z razmerjem površin pod vrhovi γ2- in γ3-kazeinov 1-odstotnega referenčnega standarda (ovca, koza) ali laboratorijskega vmesnega standarda (bivolica), analiziranega na istem gelu.
Thoroughly drain off the fixative and wash the gel plate twice for 30 seconds each time with 100 ml destaining solution (4.10).
Opomba: Metoda deluje zadovoljivo, če se pokaže jasen pozitiven znak za goveja kazeina γ2 in γ3 v 1-odstotnem referenčnem standardu, vendar ne v 0-odstotnem referenčnem standardu.
Pour off the destaining solution and fill the dish with 250 ml staining solution (4.11.3); allow to stain for 45 minutes with gentle shaking.
V nasprotnem primeru optimizirajte postopek tako, da natančno sledite podrobnostim metode.
Destaining the gel plate
Vzorec se šteje za pozitivnega, če sta oba goveja kazeina γ2 in γ3 ali ustrezajoče razmerje površin pod vrhovi enako ali večje, kot je stopnja 1-odstotnega referenčnega standarda.
Pour off the staining solution, wash the gel plate twice using a 100 ml destaining solution (4.10) each time, then shake with 200 ml destaining solution for 15 minutes and repeat the destaining step at least two or three times until the background is clear and uncoloured.
Addeo F., Nicolai M.A., Chianese L., Moio L., Spagna Musso S., Bocca A. in Del Giovine L., „A control method to detect bovine milk in ewe and water buffalo cheese using immunoblotting“, Milchwissenschaft 50, 1995, str.
Then rinse the gel plate with distilled water (2 × 2 minutes) and dry in the air (2 to 3 hours) or with a hairdryer (10 to 15 minutes).
83–85.
Note 1: Carry out fixing, washing, staining and destaining at 20 °C.
Krause I., Berner I. in Klostermeyer H., „Sensitive detection of cow milk in ewe and goat milk and cheese by carrier ampholyte – and carrier ampholyte/immobilized pH gradient – isoelectric focusing of γ-caseins using plasmin as signal amplifier“, v: Electrophoresis-Forum 89 (B. J. Radola, ur.), Bode-Verlag, München, 1989, str.
Do not use elevated temperatures.
389–393.
Note 2: If more sensitive silver staining (e.g. Silver Staining Kit, Protein, Pharmacia Biotech, Code No 17-1150-01) is preferred, plasmin-treated casein samples have to be diluted to 5 mg/ml.
Shematska risba pokrivne folije
The detection limit is less than 0,5 %.
Kazeinska plast, ki po centrifugiranju plava med vodno in organsko fazo
Visual estimation
Faza H2O
If available, apply densitometry (5.19) for the determination of the peak area ratio of bovine to ovine, caprine and/or buffalo γ2- and γ3-caseins (see Figure 5).
Poklopna tehnika za ulivanje zelo tankih poliakrilamidnih gelov
Compare this value to γ2- and γ3-casein peak area ratio of the 1 % reference standard (ewe, goat) or laboratory interim-standard (buffalo) analysed on the same gel.
a = lepilni trak na distančniku (0,25 mm); b = pokrivna folija (5.3); c, e = stekleni plošči (5.1); d = gelska raztopina (4.1.2); f = nosilna folija gela (5.2)
Note: The method is operating satisfactorily, if there is a clear positive signal for both bovine γ2- and γ3-caseins in the 1 % reference standard but not in the 0 % reference standard.
Slika 4a
If not, optimize the procedure following the details of the method precisely.
Izoelektrično fokusiranje plazminsko obdelanih kazeinov iz ovčjega in kozjega sira, ki vsebuje različne količine kravjega mleka
A sample is judged as being positive, if both bovine γ2- and γ3-caseins or the corresponding peak area ratios are equal to or greater than the level of the 1 % reference standard.
% CM = odstotek kravjega mleka, C = krava, E = ovca, G = koza
Addeo F., Moio L., Chianese L., Stingo C., Resmini P., Berner I, Krause I., Di Luccia A., Bocca A.: Use of plasmin to increase the sensitivity of the detection of bovine milk in ovine and/or caprine cheese by gel isoelectric focusing of γ2-caseins.
Prikazan je zgornji del gela izoelektričnega fokusiranja (IEF)
Milchwissenschaft 45, 708-711 (1990).
Slika 4b
Addeo F., Nicolai M.A., Chianese L., Moio L., Spagna Musso S., Bocca A., Del Giovine L.: A control method to detect bovine milk in ewe and water buffalo cheese using immunoblotting.
Izoelektrično fokusiranje plazminsko obdelanih kazeinov iz sira, narejenega iz mešanic ovčjega, kozjega in bivoličjega mleka z vsebnostjo različnih količin kravjega mleka
Milchwissenschaft 50, 83-85 (1995).
% CM = odstotek kravjega mleka; 1+ = vzorec vsebuje 1 % kravjega mleka, v sredini sledi pa ima dodatek čistega govejega kazeina.
Krause I., Berner I, Klostermeyer H.: Sensitive detection of cow milk in ewe and goat milk and cheese by carrier ampholyte — and carrier ampholyte/immobilized pH gradient — isoelectric focusing of γ-caseins using plasmin as signal amplifier. in: Electrophoresis-Forum 89 (B. J. Radola, ed.) pp 389-393, Bode-Verlag, München (1989).
C = krava, E = ovca, G = koza, B = bivol
-käse durch isoelektrische Fokussierung in harnstoffhaltigen Polyacrylamidgelen.
Prikazana je skupna razdalja separacije za gel IEF.
Radola B.J.: Ultrathin-layer isoelectric focusing in 50-100 μm polyacrylamide gels on silanised glass plates or polyester films.
Slika 5
Electrophoresis 1, 43-56 (1980).
Superpozicija denzitogramov standardnih vzorcev (SDT) in vzorcev sira, narejenega iz mešanice ovčjega, kozjega in bivoljega mleka po izometričnem fokusiranju
Schematic drawing of the covering sheet
a, b = standardi, ki vsebujejo 0 in 1 % kravjega mleka; c–g = vzorci sirov, ki vsebujejo 0, 1, 2, 3 in 7 % kravjega mleka.
Polyester sheet
C = krava, E = ovca, G = koza.
Spacer tape
Zgornja polovica gela IEF je bila obdelana pri λ = 634 nm.
Figure 2
Nanašanje vzorca: po predhodnem fokusiranju (korak 1) s pipeto nakapajte 18 μl vzorca in standardne raztopine na trakove za nanos vzorca (10 × 5 mm), jih položite na gel v razmiku 1 mm drug od drugega in 5 mm podolžno od anode ter rahlo pritisnite.
Casein layer floating between aqueous and organic phases after centrifugation
Fokusiranje opravite pod navedenimi pogoji, po 60-minutnem fokusiranju vzorca previdno odstranite trakove za nanos vzorca.
H2O-Phase
PRILOGA IX
Casein
Ocenjevanje analiz
CH2Cl2-Phase
Zagotavljanje kakovosti
Figure 3
Analize se opravijo v laboratorijih, ki so določeni v skladu s členom 12 Uredbe (ES) št. 882/2004 (**), ali jih določijo pristojni organi države članice.
Flapping technique for casting of ultrathin polyacrylamide gels
Vzorčenje in nesoglasja glede rezultatov analiz
a = spacer tape (0,25 mm); b = covering sheet (5.3); c, e = glass plates (5.1); d = gel solution (4.1.2); f = gel carrier sheet (5.2)
Vzorčenje se izvaja v skladu z ustrezno uredbo za zadevne proizvode.
Figure 4a
Če ni določb o vzorčenju, se uporabljajo določbe iz standarda ISO 707, mleko in mlečni proizvodi – smernice o vzorčenju.
Isoelectric focusing of plasmin-treated caseins from ewe's and goat's milk cheese containing different amounts of cow's milk.
Laboratorijska poročila o rezultatih analiz vsebujejo zadostne podatke za oceno rezultatov, ki se opravi v skladu z dodatkom.
% CM = percentage of cow's milk, C = cow, E = ewe, G = goat
Za analize, ki jih določajo pravila Unije, se vzamejo dvojni vzorci.
Upper half of the IEF gel is shown.
Če pride do spora glede rezultatov, plačilna agencija poskrbi za ponovno izvedbo potrebne analize na zadevnem proizvodu, stroške pa krije stranka, ki spor izgubi.
Figure 4b
Zgoraj navedena analiza se opravi, če so na voljo zapečatene kopije vzorcev proizvoda, ki jih ustrezno hranijo pristojni organi.
Isoelectric focusing of plasmin treated caseins from cheese made from mixtures of ewe's, goat's and buffalo milk containing different amounts of cow's milk.
Proizvajalec plačilni agenciji pošlje zahtevek za izvedbo analize v 7 delovnih dneh po objavi rezultatov prve analize.
% CM = percentage of cow's milk; 1 + = sample containing 1 % of cow's milk and spiked with pure bovine casein at the middle of the track.
Plačila agencija analizo izvede v 21 delovnih dneh po prejemu zahtevka.
C = cow, E = ewe, G = goat, B = buffalo.
Rezultat pritožbe je dokončen.
Total separation distance of the IEF gel is shown.
Če lahko proizvajalec v petih dneh po vzorčenju dokaže, da postopek vzorčenja ni potekal pravilno, se vzorčenje ponovi, kadar je mogoče.
Figure 5
Če vzorčenja ni mogoče ponoviti, se analizirana pošiljka sprejme.
Superposition of densitograms of standards (STD) and cheese samples made from a mixture of ewe's and goat's milk after isoelectric focusing.
Ocena skladnosti pošiljke s pravno omejitvijo
a,b = standards containing 0 and 1 % of cow's milk; c-g = cheese samples containing 0, 1, 2, 3 and 7 % of cow's milk.
Princip
C = cow, E = ewe, G = goat.
Če zakonodaja o javni intervenciji in zasebnem skladiščenju podrobno določa postopke vzorčenja, se upoštevajo navedeni postopki.
Upper half of the IEF gel was scanned at λ = 634 nm.
V vseh drugih primerih se uporabi vzorec vsaj treh vzorčnih enot, naključno izbranih iz pošiljke, ki se predloži v pregled.
Sample application: After pre-focusing (step 1), pipette 18 μl of the sample and standard solutions onto the sample applicators (10 × 5 mm), place them on the gel at 1 mm intervals from each other and 5 mm longitudinally from the anode and press lightly.
Pripravi se lahko sestavljen vzorec.
Carry out focusing using the above conditions, carefully removing the sample applicators after the 60 minutes of sample focusing.
Pridobljeni rezultat se primerja s pravnimi omejitvami z izračunom 95-odstotne stopnje natančnosti kot dvakratnega standardnega odmika, pri čemer je ustrezni standardni odmik odvisen od tega, (1) ali se metoda potrdi z mednarodnim sodelovanjem z vrednostmi za σr in σR ali (2) se v primeru znotrajlaboratorijske validacije izračuna interna obnovljivost.
ANNEX IX
Ta stopnja natančnosti bo nato enaka merilni negotovosti rezultata.
Evaluation of the analyses
Metoda je validirana z mednarodnim sodelovanjem
Quality assurance
V tem primeru se določita standardni odmik ponovljivosti σr in standardni odmik obnovljivosti σR, medtem ko lahko laboratorij dokaže skladnost z učinkovitostjo validirane metode.
Analyses shall be performed by laboratories designated in accordance with Article 12 of Regulation (EC) No 882/2004 (**) or designated by the competent authorities of the Member State.
Izračunajte aritmetično sredino za n ponovljenih meritev.
Sampling and disputes over the results of analysis
Izračunajte razširjeno negotovost (k = 2) kot
Sampling shall be carried out in accordance with the relevant regulation for the product under consideration.
Če se končni rezultat x merjenja izračuna s formulo v obliki x = y1 + y2, x = y1 – y2, x = y1 · y2 ali x = y1/y2, je treba uporabiti običajne postopke za združevanje standardnih odmikov v takšnih primerih.
If no sampling provisions are expressly provided for, then the provisions laid down in ISO 707, Milk and milk products – Guidance on sampling, shall be used.
Šteje se, da pošiljka ni v skladu z zgornjo pravno omejitvijo UL, če je
Laboratory reports of the results of the analysis shall contain sufficient information to allow an evaluation of the results to be carried out in accordance with the Appendix.
sicer se šteje, da je v skladu z UL.
Duplicate samples shall be taken for analyses required under Union rules.
Šteje se, da pošiljka ni v skladu s spodnjo pravno omejitvijo LL, če je
If a dispute arises over the results, the paying agency shall have the necessary analysis on the product in question carried out again, and the cost shall be met by the losing party.
sicer se šteje, da je v skladu z LL.
The above mentioned analysis shall be carried out provided that sealed duplicate samples of the product are available and have been stored appropriately with the competent authority.
Znotrajlaboratorijska validacija z izračunom standardnega odmika interne obnovljivosti
The manufacturer shall send a request to the paying agency to conduct the analysis within 7 working days following the notification of the results of the first analysis.
Če se uporabljajo metode, ki niso določene v tej uredbi, in niso določene meritve za natančnost, se izvede znotrajlaboratorijska validacija.
The analysis shall be carried out by the paying agency within 21 working days following receipt of the request.
V formulah za izračun razširjene negotovosti U je treba ustrezno uporabiti standardni odmik interne ponovljivosti sir in standardni odmik interne obnovljivosti siR namesto σr in σR.
The appeal result shall be the definitive one.
Pravila, ki jih je treba upoštevati za določitev skladnosti s pravno omejitvijo, so navedena v točki 1.
If the manufacturer can prove, within five working days of sampling, that the sampling procedure was not carried out correctly, sampling shall be repeated where possible.
Če se pošiljka šteje kot neskladna s pravno omejitvijo, je treba meritve ponoviti na podlagi metode iz te uredbe ter rezultat oceniti v skladu s točko 1.
If sampling cannot be repeated, the consignment shall be accepted.
Izdelka Ampholine® pH 3,5–9,5 (Pharmacia) in Resolyte® pH 5–7 in pH 6–8 (BDH, Merck) sta se pokazala kot posebno primerna za pridobitev potrebnih ločitev γ–kazeinov.
Evaluation of compliance of a consignment with the legal limit
Uredba (ES) št. 882/2004 Evropskega parlamenta in Sveta z dne 29. aprila 2004 o izvajanju uradnega nadzora, da se zagotovi preverjanje skladnosti z zakonodajo o krmi in živilih ter s pravili o zdravstvenem varstvu živali in zaščiti živali (UL L 165, 30.4.2004).
Principle
Analize sežganih delcev se lahko opravljajo sistematično.
Where public intervention and private storage legislation lay down detailed sampling procedures then those procedures shall be followed.
Vendar pa je take analize treba opraviti vedno, če se ne izvajajo senzorični pregledi.
In all other cases a sample of at least 3 sample units taken randomly from the consignment submitted to control shall be used.
Metodo, ki jo je treba uporabiti, odobri plačilna agencija (eno ali obe metodi).
A composite sample may be prepared.
Senzorični pregledi se opravijo po potrebi na podlagi analize tveganja, ki jo odobri plačilna agencija.
The result obtained shall be compared with the legal limits by calculation of a 95 % confidence interval as 2 x standard deviation, where the relevant standard deviation depends on whether (1) the method is validated through international collaboration with values for σr and σR or (2) in the case of in-house validation, an internal reproducibility has been calculated.
Sežgani delci
This confidence interval will then equate to the measurement uncertainty of the result.
Tech.
The method is validated through international collaboration
Latt.-Cas., 39, 395(1988).
In this case, the repeatability standard deviation σr and the reproducibility standard deviation σR have been established and the laboratory can demonstrate compliance with the performance characteristics of the validated method.
Minute
Calculate the arithmetic mean of the n repeated measurements.
Opomba 2: Med vsako prekinitvijo sperite kolone z vodo.
Calculate the expanded uncertainty (k = 2) of as
Mednarodni standard IDF 135B/1991.
If the final result x of measurement is calculated using a formula of the form x = y1 + y2, x = y1 – y2, x = y1 · y2 or x = y1/y2 the usual procedures for combining standard deviations in such cases shall be followed.
Mleko in mlečni proizvodi.
The consignment is judged to be not in compliance with the upper legal limit UL if
(min)
otherwise it is judged to be in compliance with UL.
pribl.
The consignment is judged to be not in compliance with the lower legal limit LL if
300
In-house validation with calculation of internal reproducibility standard deviation
1000
In cases where methods not specified in this Regulation are used and precision measures have not been established, an in-house validation shall be carried out.
pribl.
Internal repeatability standard deviation σir and the internal reproducibility standard deviation σiR shall be used instead of σr and σR, resp., in the formulae for the computation of the expanded uncertainty U.
3000
However, if the consignment is judged to be non-compliant with the legal limit, the measurements shall be repeated with the method specified in this Regulation and the result evaluated in accordance to point 1.
195–199.
1).
(minute)
Scorched particles' analyses may be conducted systematically.
Opomba 1: Retenzijski čas CMPA je 26 ± 2 minuti.
However, such analyses shall always be conducted if no sensory checks are performed.
Opomba 2: Eluente je treba primerno razpliniti in morajo ostati razplinjeni.
The method to be applied shall be approved by the paying agency (one or both methods).
Opomba 3: Vsakih pet vzorcev je treba vbrizgati referenčni vzorec [5] in ga uporabiti za izračun novega odzivnega faktorja R (9.1.1).
Sensory checks shall be performed where deemed necessary after risk based analysis approved by the paying agency.
Eluent B: V 1000-mililitrsko merilno bučko dodajte 550 ml acetonitrila, 20 ml izopropanola in 1,00 ml TFA.
Scorched particles
9.4.3.2